Rh5DG083300

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012808
Physical Location & Seq
Forward (+)
7330346 .. 7333214
2869 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG083300.1

Sequence Viewer

Length: 1182 bp
ATGAAAAAGAAGCTAGAGCTGCCTCTTGGGTTTTGCTTCTTCTACTTGTGCATCCCATTGGTCACAGCTTCTGACAGTTTGCTCTCCCCCAAAGGTGTCAACTTCGAAGTGGCTGCATTGATGTCAGTGAAGAAGGAGATGCAGGATGAGTTGCTTGTGATGGGTGGGTGGGATATCAACTCAGTTGATCCTTGCACTTGGAACATGGTGGGTTGTTCTGCTGAGGGATATGTAATCTCCGTTGCAATGGCCAGCTCGGGTTTATCTGGAATGCTTTCGCCAAGTATTGGGAATTTGAGTCATCTTCGGACATTGTTGTTACAAAACAATCAGTTGTCTGGTCCTATCCCAACTGAGATAGGGAGGCTTTCGCTGCTTGAAACTCTGGACCTTTCGGGTAACCAGTTTTCTGGAAACATTCCGAGTTCTTTGGGGTTTCTGACTCATCTAAGTTACTTGAGGCTTAATAGAAATAATTTGAGCGGTCAAATTCCTAGCCTTGTTGCTGATCTCACAGATCTTTCATTCTTGGATTTATCATTTAATAATCTTAGTGGGCCCACGCCAAAAATACTGGCGAAAGGCTACAGTGTGACAGGGAACAGCTTTCTTTGCAATCCTTTGTCAGCAGACTCAACACGAATCTGCGTGGATGTGTCAAAACCAGTAAACGACACAGGTTCACCACCGAAAGTCAGTGACCACCATCGATGGGTACTTTCTGTTGCTCTAGGCATTAGTTGTGCATTTGTTATTTCAGTGATGCTACTTGTTTGTTGGGTGCATTGGTACAGATCTCGTCTTCTATTTACATCTTATGTGCAGCAAGATTATGAATTAGATATAAGTCATCTAAAGAGGTTCTCCTATCGTGAATTACAACTTGCAACGAGCAATTTTAGTTCTAAAAACATCCTAGGACAAGGTGGATATGGAGTCGTTTATAAAGGATGTCTTCCTAATAGGACACTGGTGGCAGTTAAGAGACTTAGAGATCCCAATTTCACTGGAGAAGTTCAGTTCCAAACTGAAGTTGAAATGATAGGCTTGGCTTTGCATCGGAACCTTTTACGTTTATTTGGATTTTGTATGACAACAAACGAGAGGTTGCTTGTTTATCCTTATATGCCAAATGGGAGTGTTGCTGACCGGTTAAGAGGTCTGTTTCGTTTCTTTGCTTGA

Protein Analysis

393

Amino Acids

43.43

Weight (kDa)

8.47

Isoelectric Point (pI)

30.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 73 4.7e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 95 - 180 4.1e-13 Leucine-rich repeat region
Pkinase PF00069 301 - 386 2.4e-10 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 303 - 386 3e-11 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012808)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45780 AT5G45780
fragaria_vesca FvH4_3g07470 FvH4_3g07470 FvH4_3g07470
malus_domestica MD05G1297400.v1.1 MD10G1277100.v1.1
prunus_persica Prupe.4G064800_v2.0.a1
pyrus_communis pycom05g27550 pycom10g23140
rosa_chinensis RchiOBHm_Chr5g0011381
rosa_laevigata RLG00000031828
rosa_multiflora Rmu_sc0009975.1_g000012
rosa_roxburghii Rroxscaffold_1G00065070
rosa_rugosa Rorug04G0456700
rosa_samantha Rh5BG083400 Rh5DG083300
rosa_wichuraiana Rw5G007670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 945
AccB7I CCANNNNNTGG 1 cut(s) 287
AccBSI CCGCTC 1 cut(s) 483
AciI CCGC 1 cut(s) 483
AclWI GGATC 2 cut(s) 182, 989
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 2 cut(s) 292, 489
AcuI CTGAAG 1 cut(s) 1050
AfaI GTAC 2 cut(s) 717, 791
AfiI CCNNNNNNNGG 2 cut(s) 287, 712
AgeI ACCGGT 1 cut(s) 1149
AgsI TTSAA 2 cut(s) 380, 1037
AluBI AGCT 5 cut(s) 13, 19, 68, 255, 606
AluI AGCT 5 cut(s) 13, 19, 68, 255, 606
Alw26I GTCTC 1 cut(s) 979
AlwI GGATC 2 cut(s) 182, 989
AlwNI CAGNNNCTG 1 cut(s) 71
Ama87I CYCGRG 1 cut(s) 256
AoxI GGCC 2 cut(s) 249, 557
ApaI GGGCCC 1 cut(s) 561
ApeKI GCWGC 4 cut(s) 19, 113, 373, 823
ApoI RAATTY 2 cut(s) 292, 489
AsiGI ACCGGT 1 cut(s) 1149
Asp700I GAANNNNTTC 1 cut(s) 274
AspA2I CCTAGG 1 cut(s) 916
AspS9I GGNCC 4 cut(s) 341, 388, 557, 558
AsuHPI GGTGA 1 cut(s) 675
AsuII TTCGAA 1 cut(s) 105
AvaI CYCGRG 1 cut(s) 256
AvaII GGWCC 2 cut(s) 341, 388
AvrII CCTAGG 1 cut(s) 916
BaeGI GKGCMC 1 cut(s) 561
BalI TGGCCA 1 cut(s) 251
BanII GRGCYC 1 cut(s) 561
BbsI GAAGAC 2 cut(s) 794, 947
BbvCI CCTCAGC 1 cut(s) 222
BbvI GCAGC 4 cut(s) 6, 100, 360, 835
BccI CCATC 3 cut(s) 154, 705, 714
BcgI CGANNNNNNTGC 2 cut(s) 95, 129
BcoDI GTCTC 1 cut(s) 979
BfaI CTAG 4 cut(s) 14, 495, 731, 917
BfmI CTRYAG 1 cut(s) 586
BglII AGATCT 2 cut(s) 517, 794
BisI GCNGC 4 cut(s) 20, 114, 374, 824
BlnI CCTAGG 1 cut(s) 916
BlsI GCNGC 4 cut(s) 21, 115, 375, 825
Bme18I GGWCC 2 cut(s) 341, 388
BmeT110I CYCGRG 1 cut(s) 256
BmgT120I GGNCC 4 cut(s) 341, 388, 557, 558
BmiI GGNNCC 2 cut(s) 559, 1064
BmsI GCATC 4 cut(s) 60, 129, 753, 1066
BpiI GAAGAC 2 cut(s) 794, 947
BpmI CTGGAG 1 cut(s) 1029
Bpu10I CCTNAGC 1 cut(s) 222
Bpu14I TTCGAA 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 478
Bsa29I ATCGAT 1 cut(s) 709
BsaJI CCNNGG 1 cut(s) 916
BsaWI WCCGGW 1 cut(s) 1149
Bsc4I CCNNNNNNNGG 2 cut(s) 287, 712
Bse118I RCCGGY 1 cut(s) 1149
Bse1I ACTGG 5 cut(s) 403, 579, 665, 975, 1012
Bse3DI GCAATG 1 cut(s) 252
BseCI ATCGAT 1 cut(s) 709
BseDI CCNNGG 1 cut(s) 916
BseGI GGATG 5 cut(s) 51, 151, 658, 912, 956
BseLI CCNNNNNNNGG 2 cut(s) 287, 712
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 3 cut(s) 195, 213, 345
BseNI ACTGG 5 cut(s) 403, 579, 665, 975, 1012
BseSI GKGCMC 1 cut(s) 561
BseXI GCAGC 4 cut(s) 6, 100, 360, 835
BsgI GTGCAG 1 cut(s) 842
BshFI GGCC 2 cut(s) 251, 559
BshTI ACCGGT 1 cut(s) 1149
BshVI ATCGAT 1 cut(s) 709
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 1 cut(s) 1150
BslI CCNNNNNNNGG 2 cut(s) 287, 712
BsmAI GTCTC 1 cut(s) 979
BsmI GAATGC 1 cut(s) 276
BsnI GGCC 2 cut(s) 251, 559
BsoBI CYCGRG 1 cut(s) 256
Bsp119I TTCGAA 1 cut(s) 105
Bsp120I GGGCCC 1 cut(s) 557
Bsp1286I GDGCHC 1 cut(s) 561
Bsp143I GATC 5 cut(s) 187, 508, 517, 794, 994
BspACI CCGC 1 cut(s) 483
BspANI GGCC 2 cut(s) 251, 559
BspCNI CTCAG 3 cut(s) 194, 214, 346
BspDI ATCGAT 1 cut(s) 709
BspLI GGNNCC 2 cut(s) 559, 1064
BspPI GGATC 2 cut(s) 182, 989
BspT104I TTCGAA 1 cut(s) 105
BsrBI CCGCTC 1 cut(s) 483
BsrDI GCAATG 1 cut(s) 252
BsrFI RCCGGY 1 cut(s) 1149
BsrI ACTGG 5 cut(s) 403, 579, 665, 975, 1012
BssAI RCCGGY 1 cut(s) 1149
BssECI CCNNGG 1 cut(s) 916
BssMI GATC 5 cut(s) 187, 508, 517, 794, 994
BssT1I CCWWGG 1 cut(s) 916
Bst4CI ACNGT 2 cut(s) 77, 590
BstBI TTCGAA 1 cut(s) 105
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 6 cut(s) 181, 222, 354, 449, 551, 989
BstEII GGTNACC 1 cut(s) 398
BstF5I GGATG 5 cut(s) 51, 151, 658, 912, 956
BstKTI GATC 5 cut(s) 190, 511, 520, 797, 997
BstMAI GTCTC 1 cut(s) 979
BstMBI GATC 5 cut(s) 187, 508, 517, 794, 994
BstMWI GCNNNNNNNGC 3 cut(s) 19, 373, 612
BstPI GGTNACC 1 cut(s) 398
BstSFI CTRYAG 1 cut(s) 586
BstSLI GKGCMC 1 cut(s) 561
BstV1I GCAGC 4 cut(s) 6, 100, 360, 835
BstV2I GAAGAC 2 cut(s) 794, 947
BstX2I RGATCY 3 cut(s) 517, 794, 994
BstXI CCANNNNNNTGG 1 cut(s) 410
BstYI RGATCY 3 cut(s) 517, 794, 994
Bsu15I ATCGAT 1 cut(s) 709
BsuRI GGCC 2 cut(s) 251, 559
BsuTUI ATCGAT 1 cut(s) 709
BtsCI GGATG 5 cut(s) 51, 151, 658, 912, 956
BtsIMutI CAGTG 6 cut(s) 132, 595, 703, 765, 968, 1005
Cac8I GCNNGC 1 cut(s) 253
CaiI CAGNNNCTG 1 cut(s) 71
Cfr10I RCCGGY 1 cut(s) 1149
Cfr13I GGNCC 4 cut(s) 341, 388, 557, 558
ClaI ATCGAT 1 cut(s) 709
Csp6I GTAC 2 cut(s) 716, 790
CspAI ACCGGT 1 cut(s) 1149
CviAII CATG 1 cut(s) 205
CviQI GTAC 2 cut(s) 716, 790
DdeI CTNAG 6 cut(s) 181, 222, 354, 449, 551, 989
DpnI GATC 5 cut(s) 189, 510, 519, 796, 996
DpnII GATC 5 cut(s) 187, 508, 517, 794, 994
EaeI YGGCCR 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 916
Eco24I GRGCYC 1 cut(s) 561
Eco32I GATATC 1 cut(s) 175
Eco47I GGWCC 2 cut(s) 341, 388
Eco57I CTGAAG 1 cut(s) 1050
Eco88I CYCGRG 1 cut(s) 256
Eco91I GGTNACC 1 cut(s) 398
EcoO65I GGTNACC 1 cut(s) 398
EcoRV GATATC 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 916
EcoT38I GRGCYC 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 916
FaeI CATG 1 cut(s) 208
FalI AAGNNNNNCTT 2 cut(s) 939, 971
FatI CATG 1 cut(s) 204
Fnu4HI GCNGC 4 cut(s) 20, 114, 374, 824
FokI GGATG 5 cut(s) 38, 158, 665, 899, 963
FriOI GRGCYC 1 cut(s) 561
Fsp4HI GCNGC 4 cut(s) 20, 114, 374, 824
FspBI CTAG 4 cut(s) 14, 495, 731, 917
GluI GCNGC 4 cut(s) 20, 114, 374, 824
GsuI CTGGAG 1 cut(s) 1029
HaeIII GGCC 2 cut(s) 251, 559
HapII CCGG 1 cut(s) 1150
Hin1II CATG 1 cut(s) 208
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 5 cut(s) 298, 442, 632, 642, 936
HpaII CCGG 1 cut(s) 1150
HphI GGTGA 1 cut(s) 675
Hpy166II GTNNAC 3 cut(s) 100, 670, 683
Hpy188I TCNGA 5 cut(s) 73, 309, 423, 441, 1062
Hpy188III TCNNGA 4 cut(s) 267, 386, 411, 872
Hpy8I GTNNAC 3 cut(s) 100, 670, 683
HpyAV CCTTC 1 cut(s) 127
HpyCH4III ACNGT 2 cut(s) 77, 590
HpyCH4IV ACGT 1 cut(s) 1072
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 373, 612
HpyF3I CTNAG 6 cut(s) 181, 222, 354, 449, 551, 989
HpySE526I ACGT 1 cut(s) 1072
Hsp92II CATG 1 cut(s) 208
Kzo9I GATC 5 cut(s) 187, 508, 517, 794, 994
Lsp1109I GCAGC 4 cut(s) 6, 100, 360, 835
LweI GCATC 4 cut(s) 60, 129, 753, 1066
MaeI CTAG 4 cut(s) 14, 495, 731, 917
MaeII ACGT 1 cut(s) 1072
MaeIII GTNAC 6 cut(s) 61, 318, 398, 452, 592, 698
MalI GATC 5 cut(s) 189, 510, 519, 796, 996
MbiI CCGCTC 1 cut(s) 483
MboI GATC 5 cut(s) 187, 508, 517, 794, 994
MboII GAAGA 5 cut(s) 31, 142, 296, 794, 947
MflI RGATCY 3 cut(s) 517, 794, 994
MhlI GDGCHC 1 cut(s) 561
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 7 cut(s) 292, 475, 489, 836, 875, 895, 1000
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 4 cut(s) 307, 436, 626, 945
MnlI CCTC 7 cut(s) 33, 217, 357, 453, 852, 1098, 1151
Mox20I TGGCCA 1 cut(s) 251
MroXI GAANNNNTTC 1 cut(s) 274
MscI TGGCCA 1 cut(s) 251
MseI TTAA 4 cut(s) 465, 543, 981, 1154
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 1 cut(s) 1150
Mva1269I GAATGC 1 cut(s) 276
MwoI GCNNNNNNNGC 3 cut(s) 19, 373, 612
NdeII GATC 5 cut(s) 187, 508, 517, 794, 994
NlaIII CATG 1 cut(s) 208
NlaIV GGNNCC 2 cut(s) 559, 1064
NmuCI GTSAC 3 cut(s) 61, 592, 698
NspV TTCGAA 1 cut(s) 105
PctI GAATGC 1 cut(s) 276
PdmI GAANNNNTTC 1 cut(s) 274
PfeI GAWTC 1 cut(s) 642
PflMI CCANNNNNTGG 1 cut(s) 287
PinAI ACCGGT 1 cut(s) 1149
PkrI GCNGC 4 cut(s) 21, 115, 375, 825
PleI GAGTC 4 cut(s) 306, 436, 626, 944
PpsI GAGTC 4 cut(s) 306, 436, 626, 944
PsiI TTATAA 1 cut(s) 945
PspEI GGTNACC 1 cut(s) 398
PspN4I GGNNCC 2 cut(s) 559, 1064
PspOMI GGGCCC 1 cut(s) 557
PspPI GGNCC 4 cut(s) 341, 388, 557, 558
PstNI CAGNNNCTG 1 cut(s) 71
PsuI RGATCY 3 cut(s) 517, 794, 994
RsaI GTAC 2 cut(s) 717, 791
RsaNI GTAC 2 cut(s) 716, 790
SaqAI TTAA 4 cut(s) 465, 543, 981, 1154
SatI GCNGC 4 cut(s) 20, 114, 374, 824
Sau3AI GATC 5 cut(s) 187, 508, 517, 794, 994
Sau96I GGNCC 4 cut(s) 341, 388, 557, 558
SchI GAGTC 4 cut(s) 307, 436, 626, 945
SduI GDGCHC 1 cut(s) 561
SfaNI GCATC 4 cut(s) 60, 129, 753, 1066
SfcI CTRYAG 1 cut(s) 586
SfuI TTCGAA 1 cut(s) 105
SinI GGWCC 2 cut(s) 341, 388
SmlI CTYRAG 1 cut(s) 457
SmoI CTYRAG 1 cut(s) 457
Sse9I AATT 7 cut(s) 292, 475, 489, 836, 875, 895, 1000
SsiI CCGC 1 cut(s) 483
SspMI CTAG 4 cut(s) 14, 495, 731, 917
StyI CCWWGG 1 cut(s) 916
TaaI ACNGT 2 cut(s) 77, 590
TaiI ACGT 1 cut(s) 1075
TaqI TCGA 2 cut(s) 105, 709
TasI AATT 7 cut(s) 292, 475, 489, 836, 875, 895, 1000
TfiI GAWTC 1 cut(s) 642
Tru1I TTAA 4 cut(s) 465, 543, 981, 1154
Tru9I TTAA 4 cut(s) 465, 543, 981, 1154
TscAI CASTG 6 cut(s) 132, 595, 703, 765, 975, 1012
TseFI GTSAC 3 cut(s) 61, 592, 698
TseI GCWGC 4 cut(s) 19, 113, 373, 823
Tsp45I GTSAC 3 cut(s) 61, 592, 698
TspDTI ATGAA 3 cut(s) 17, 513, 849
TspGWI ACGGA 1 cut(s) 229
TspRI CASTG 6 cut(s) 132, 595, 703, 765, 975, 1012
Van91I CCANNNNNTGG 1 cut(s) 287
VpaK11BI GGWCC 2 cut(s) 341, 388
XapI RAATTY 2 cut(s) 292, 489
XmaJI CCTAGG 1 cut(s) 916
XmnI GAANNNNTTC 1 cut(s) 274
XspI CTAG 4 cut(s) 14, 495, 731, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.