Rh5DG419900

Cytochrome B561, N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0016195
Physical Location & Seq
Forward (+)
66867187 .. 66871870
4684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG419900.1

Sequence Viewer

Length: 1518 bp
ATGGAGGGCAGAGACAACAAGGGCTCCTCGCCCCCACCAAAGCCCTCCAAGTTCTCGGTCTACCAGAACCCAACCCTCTCCGCCGCCCTCACCGCCAGCAGCCTCCGACCTCCCAAGCACGCCCTCCTCGTCATCTTCTCTCTCTCGTCCCTCTCCGCCGTCGCCTTCCTCGTCATTCTCTCCAGGGAGAATGAGTTTGTTAACAATTTGAAGCTCAATATTCTTTCCCAAGAGGCAGCCTATTTGTTTGTTAAAGTGGTACATACTGTTGTGGGATTAGCATTTTTGGCTACTCTTGTTGCATTCTTCAGAGTCATCTCGTTGAGAAATGCCGCTGTTGTGCCAACTGTATCTCCCTCCAAAGGAACCAAGGATAAGATGGGCTTGACAAGTCGTCAGCTTGGACTTCTAGGAATTAAGCCAAAGGTTGAACAAGTTGTATCCGAGTCTGCAAAGAAACCTCCCAAGTCCAAGCCCTACTTGTCTTCCCCTTCAGATGTCCTTGTTCCACTTCATCAGCCAATCAGCAGTTCAAATCGTCTGTCTCGAATTGGCTCAGATAAATTTAACACCGGTGGTGGAAATAAGATGGGATCCATCACCAGTCCTTCTAAATCACCCAGTTCCGCGTCTTCTTTGTATCTTGTCCCAGGAGCTGTTTCACCAATGCCATCTGTTCATAATTCACCTGGAGTGGATTCAGTGGTCTCTTCTCCTTGGTCAAGTAAGCGAGCCCCTGGAAGAGAGATAACGTCGGAAGAAAAGTTTGAACAGTTCTTGGCAGATGTTGATGAGAAAATCACACAATCAGCAGGAAAACTGGCAACTCCACCACCCACTATCAGAGGCTTTGGTGTAGCCAGTCCCAGTTCAGCTAATACATCTGGAACTACAAGGAGTACACCACTGAGGCCTGTGAGAATGTCTCCAGGTTCCCAGAAGTTCAGCACTCCCCCAAAGAAAGGGGAAGGTGAGCTTCCTCCACCTATGTCCATGGAAGAATCTATTAATGCATTTGATCGTTTGGGCATCTATCCTCAAATCGAGCAGTGGCGTGACAACCTCAGGCAATGGTTTTCTTCAGTTTTGCTTAATCCCCTACTCCATAAGATTGAGAACAGTCATATTCAGGTAATGGAAGCAGCTTCCAAACTTGGGATATCACTCACAATTAGTCAAATTGGATGTGATTTACCAACTACTGGAACTACTTCAGTGTCTTCAACTGACAGGACTAAGGAATGGCAACAAACATTAACTCTTGATGAAGATGGAGTTATGCATCAAGTACGAGCTACTCTCCTCCAGTATATTAATGCTTCCACCTCACAGCTGCCTCAAGCAAATCTCCAACAAACCCCACAGCAGAATATGGTGCCTATTATGCAGGAGTGTGTCGATGCGATCACTGAACACCAGAGGCTACATGCATTGATGAAGGGAGAGTTAATTAAAGGTTTGCTACCGCAAAGCAGCATTCGAGCAGAATACACAGTACAAAGGATTCAAGGTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CytochromB561_N

Protein Analysis

505

Amino Acids

54.58

Weight (kDa)

9.64

Isoelectric Point (pI)

56.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CytochromB561_N PF09786 17 - 503 3.8e-130 Cytochrome B561, N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016195)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07970
fragaria_vesca FvH4_3g32920
prunus_persica Prupe.6G097500_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0060051
rosa_laevigata RLG00000035342
rosa_multiflora Rmu_sc0000493.1_g000021 Rmu_sc0000493.1_g000024 Rmu_sc0003366.1_g000027
rosa_roxburghii Rroxscaffold_1G00020590
rosa_rugosa Rorug05G0327900
rosa_samantha Rh5AG391700 Rh5BG404400 Rh5CG428000 Rh5DG419900
rosa_wichuraiana Rw5G036910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1375
AccB7I CCANNNNNTGG 1 cut(s) 1202
AccI GTMKAC 1 cut(s) 60
AccII CGCG 1 cut(s) 629
AciI CCGC 7 cut(s) 81, 84, 93, 156, 333, 627, 1466
AclWI GGATC 2 cut(s) 588, 601
AcsI RAATTY 1 cut(s) 563
AcuI CTGAAG 4 cut(s) 292, 477, 1065, 1197
AfaI GTAC 5 cut(s) 261, 901, 1290, 1497, 1513
AfiI CCNNNNNNNGG 4 cut(s) 362, 962, 1155, 1202
AgeI ACCGGT 1 cut(s) 572
AgsI TTSAA 6 cut(s) 211, 431, 534, 770, 1224, 1508
AhdI GACNNNNNGTC 1 cut(s) 393
AjnI CCWGG 5 cut(s) 182, 649, 688, 736, 928
AjuI GAANNNNNNNTTGG 2 cut(s) 514, 546
AluBI AGCT 8 cut(s) 214, 400, 656, 875, 976, 1145, 1295, 1333
AluI AGCT 8 cut(s) 214, 400, 656, 875, 976, 1145, 1295, 1333
Alw26I GTCTC 4 cut(s) 6, 549, 712, 930
AlwI GGATC 2 cut(s) 588, 601
AlwNI CAGNNNCTG 1 cut(s) 656
AoxI GGCC 1 cut(s) 911
ApeKI GCWGC 5 cut(s) 99, 236, 1142, 1333, 1473
ApoI RAATTY 1 cut(s) 563
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
AseI ATTAAT 2 cut(s) 1008, 1314
AsiGI ACCGGT 1 cut(s) 572
Asp700I GAANNNNTTC 1 cut(s) 1210
AsuHPI GGTGA 6 cut(s) 82, 592, 609, 654, 678, 983
AxyI CCTNAGG 1 cut(s) 1064
BamHI GGATCC 1 cut(s) 593
BanI GGYRCC 1 cut(s) 1375
BanII GRGCYC 2 cut(s) 26, 736
BbsI GAAGAC 3 cut(s) 477, 624, 1212
BbvI GCAGC 5 cut(s) 111, 248, 1154, 1320, 1485
BccI CCATC 5 cut(s) 373, 583, 605, 679, 1265
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 5 cut(s) 184, 651, 690, 738, 930
BciVI GTATCC 1 cut(s) 451
BcoDI GTCTC 4 cut(s) 6, 549, 712, 930
BfaI CTAG 1 cut(s) 410
BfuI GTATCC 1 cut(s) 451
BisI GCNGC 7 cut(s) 84, 100, 237, 333, 1143, 1334, 1474
BlsI GCNGC 7 cut(s) 85, 101, 238, 334, 1144, 1335, 1475
Bme1390I CCNGG 5 cut(s) 184, 651, 690, 738, 930
BmeRI GACNNNNNGTC 1 cut(s) 393
BmiI GGNNCC 5 cut(s) 25, 367, 595, 934, 1377
BmrFI CCNGG 5 cut(s) 184, 651, 690, 738, 930
BmrI ACTGGG 2 cut(s) 615, 861
BmsI GCATC 3 cut(s) 1038, 1291, 1390
BmuI ACTGGG 2 cut(s) 615, 861
BpiI GAAGAC 3 cut(s) 477, 624, 1212
BplI GAGNNNNNCTC 4 cut(s) 910, 942, 1284, 1316
BpmI CTGGAG 4 cut(s) 166, 711, 912, 1289
BpuEI CTTGAG 1 cut(s) 1323
BsaI GGTCTC 1 cut(s) 712
BsaJI CCNNGG 6 cut(s) 183, 369, 649, 716, 736, 993
BsaWI WCCGGW 1 cut(s) 572
BsaXI ACNNNNNCTCC 6 cut(s) 8, 38, 179, 209, 337, 367
Bsc4I CCNNNNNNNGG 4 cut(s) 362, 962, 1155, 1202
Bse118I RCCGGY 1 cut(s) 572
Bse1I ACTGG 7 cut(s) 603, 621, 825, 861, 867, 1207, 1306
Bse21I CCTNAGG 1 cut(s) 1064
Bse3DI GCAATG 1 cut(s) 1076
BseBI CCWGG 5 cut(s) 184, 651, 690, 738, 930
BseDI CCNNGG 6 cut(s) 183, 369, 649, 716, 736, 993
BseGI GGATG 1 cut(s) 1190
BseLI CCNNNNNNNGG 4 cut(s) 362, 962, 1155, 1202
BseMI GCAATG 1 cut(s) 1076
BseMII CTCAG 3 cut(s) 570, 899, 1078
BseNI ACTGG 7 cut(s) 603, 621, 825, 861, 867, 1207, 1306
BseRI GAGGAG 3 cut(s) 16, 116, 1292
BseXI GCAGC 5 cut(s) 111, 248, 1154, 1320, 1485
Bsh1236I CGCG 1 cut(s) 629
BshFI GGCC 1 cut(s) 913
BshNI GGYRCC 1 cut(s) 1375
BshTI ACCGGT 1 cut(s) 572
BsiSI CCGG 1 cut(s) 573
BslFI GGGAC 3 cut(s) 133, 632, 849
BslI CCNNNNNNNGG 4 cut(s) 362, 962, 1155, 1202
BsmAI GTCTC 4 cut(s) 6, 549, 712, 930
BsmFI GGGAC 3 cut(s) 133, 632, 849
BsmI GAATGC 2 cut(s) 302, 1476
BsnI GGCC 1 cut(s) 913
Bso31I GGTCTC 1 cut(s) 712
Bsp1286I GDGCHC 2 cut(s) 26, 736
Bsp143I GATC 3 cut(s) 593, 1018, 1404
Bsp19I CCATGG 1 cut(s) 993
BspACI CCGC 7 cut(s) 81, 84, 93, 156, 333, 627, 1466
BspANI GGCC 1 cut(s) 913
BspCNI CTCAG 3 cut(s) 569, 900, 1077
BspFNI CGCG 1 cut(s) 629
BspLI GGNNCC 5 cut(s) 25, 367, 595, 934, 1377
BspPI GGATC 2 cut(s) 588, 601
BspT107I GGYRCC 1 cut(s) 1375
BspTNI GGTCTC 1 cut(s) 712
BsrDI GCAATG 1 cut(s) 1076
BsrFI RCCGGY 1 cut(s) 572
BsrI ACTGG 7 cut(s) 603, 621, 825, 861, 867, 1207, 1306
BssAI RCCGGY 1 cut(s) 572
BssECI CCNNGG 6 cut(s) 183, 369, 649, 716, 736, 993
BssMI GATC 3 cut(s) 593, 1018, 1404
BssT1I CCWWGG 3 cut(s) 369, 716, 993
Bst2UI CCWGG 5 cut(s) 184, 651, 690, 738, 930
Bst4CI ACNGT 5 cut(s) 268, 349, 774, 1121, 1495
Bst6I CTCTTC 2 cut(s) 715, 736
BstC8I GCNNGC 3 cut(s) 97, 120, 732
BstDEI CTNAG 4 cut(s) 556, 908, 1064, 1236
BstDSI CCRYGG 1 cut(s) 993
BstF5I GGATG 1 cut(s) 1190
BstFNI CGCG 1 cut(s) 629
BstKTI GATC 3 cut(s) 596, 1021, 1407
BstMAI GTCTC 4 cut(s) 6, 549, 712, 930
BstMBI GATC 3 cut(s) 593, 1018, 1404
BstMWI GCNNNNNNNGC 3 cut(s) 92, 287, 1384
BstNI CCWGG 5 cut(s) 184, 651, 690, 738, 930
BstNSI RCATGY 1 cut(s) 1430
BstSCI CCNGG 5 cut(s) 182, 649, 688, 736, 928
BstUI CGCG 1 cut(s) 629
BstV1I GCAGC 5 cut(s) 111, 248, 1154, 1320, 1485
BstV2I GAAGAC 3 cut(s) 477, 624, 1212
BstX2I RGATCY 1 cut(s) 593
BstYI RGATCY 1 cut(s) 593
Bsu36I CCTNAGG 1 cut(s) 1064
BsuI GTATCC 1 cut(s) 451
BsuRI GGCC 1 cut(s) 913
BtgI CCRYGG 1 cut(s) 993
BtsCI GGATG 1 cut(s) 1190
BtsI GCAGTG 1 cut(s) 1055
BtsIMutI CAGTG 5 cut(s) 708, 905, 1055, 1221, 1407
Cac8I GCNNGC 3 cut(s) 97, 120, 732
CaiI CAGNNNCTG 1 cut(s) 656
Cfr10I RCCGGY 1 cut(s) 572
CseI GACGC 1 cut(s) 618
Csp6I GTAC 5 cut(s) 260, 900, 1289, 1496, 1512
CspAI ACCGGT 1 cut(s) 572
CviAII CATG 2 cut(s) 994, 1427
CviQI GTAC 5 cut(s) 260, 900, 1289, 1496, 1512
DdeI CTNAG 4 cut(s) 556, 908, 1064, 1236
DpnI GATC 3 cut(s) 595, 1020, 1406
DpnII GATC 3 cut(s) 593, 1018, 1404
DriI GACNNNNNGTC 1 cut(s) 393
Eam1104I CTCTTC 2 cut(s) 715, 736
Eam1105I GACNNNNNGTC 1 cut(s) 393
EarI CTCTTC 2 cut(s) 715, 736
EciI GGCGGA 2 cut(s) 70, 145
Eco130I CCWWGG 3 cut(s) 369, 716, 993
Eco147I AGGCCT 1 cut(s) 913
Eco24I GRGCYC 2 cut(s) 26, 736
Eco31I GGTCTC 1 cut(s) 712
Eco32I GATATC 1 cut(s) 1161
Eco57I CTGAAG 4 cut(s) 292, 477, 1065, 1197
Eco81I CCTNAGG 1 cut(s) 1064
EcoRII CCWGG 5 cut(s) 182, 649, 688, 736, 928
EcoRV GATATC 1 cut(s) 1161
EcoT14I CCWWGG 3 cut(s) 369, 716, 993
EcoT22I ATGCAT 3 cut(s) 1015, 1284, 1432
EcoT38I GRGCYC 2 cut(s) 26, 736
ErhI CCWWGG 3 cut(s) 369, 716, 993
FaeI CATG 2 cut(s) 997, 1430
FalI AAGNNNNNCTT 6 cut(s) 368, 400, 464, 496, 960, 992
FaqI GGGAC 3 cut(s) 133, 632, 849
FatI CATG 2 cut(s) 993, 1426
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 7 cut(s) 84, 100, 237, 333, 1143, 1334, 1474
FokI GGATG 1 cut(s) 1197
FriOI GRGCYC 2 cut(s) 26, 736
Fsp4HI GCNGC 7 cut(s) 84, 100, 237, 333, 1143, 1334, 1474
FspBI CTAG 1 cut(s) 410
GluI GCNGC 7 cut(s) 84, 100, 237, 333, 1143, 1334, 1474
GsuI CTGGAG 4 cut(s) 166, 711, 912, 1289
HaeIII GGCC 1 cut(s) 913
HapII CCGG 1 cut(s) 573
HgaI GACGC 1 cut(s) 618
Hin1II CATG 2 cut(s) 997, 1430
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 5 cut(s) 312, 446, 698, 1001, 1504
HpaI GTTAAC 1 cut(s) 202
HpaII CCGG 1 cut(s) 573
HphI GGTGA 6 cut(s) 82, 592, 609, 654, 678, 983
Hpy166II GTNNAC 3 cut(s) 61, 202, 902
Hpy188I TCNGA 7 cut(s) 107, 311, 445, 496, 559, 757, 845
Hpy188III TCNNGA 3 cut(s) 546, 885, 1262
Hpy8I GTNNAC 3 cut(s) 61, 202, 902
Hpy99I CGWCG 2 cut(s) 164, 757
HpyAV CCTTC 5 cut(s) 175, 501, 618, 962, 1432
HpyCH4III ACNGT 5 cut(s) 268, 349, 774, 1121, 1495
HpyCH4IV ACGT 1 cut(s) 752
HpyCH4V TGCA 6 cut(s) 302, 452, 1013, 1282, 1387, 1430
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 287, 1384
HpyF3I CTNAG 4 cut(s) 556, 908, 1064, 1236
HpySE526I ACGT 1 cut(s) 752
Hsp92II CATG 2 cut(s) 997, 1430
KspAI GTTAAC 1 cut(s) 202
Kzo9I GATC 3 cut(s) 593, 1018, 1404
LmnI GCTCC 2 cut(s) 29, 653
Lsp1109I GCAGC 5 cut(s) 111, 248, 1154, 1320, 1485
LweI GCATC 3 cut(s) 1038, 1291, 1390
MaeI CTAG 1 cut(s) 410
MaeII ACGT 1 cut(s) 752
MaeIII GTNAC 1 cut(s) 1055
MalI GATC 3 cut(s) 595, 1020, 1406
MboI GATC 3 cut(s) 593, 1018, 1404
MflI RGATCY 1 cut(s) 593
MhlI GDGCHC 2 cut(s) 26, 736
MluCI AATT 8 cut(s) 205, 414, 549, 563, 682, 1170, 1179, 1449
MlyI GAGTC 2 cut(s) 321, 455
MmeI TCCRAC 3 cut(s) 130, 735, 1375
Mph1103I ATGCAT 3 cut(s) 1015, 1284, 1432
MroXI GAANNNNTTC 1 cut(s) 1210
MspA1I CMGCKG 2 cut(s) 335, 1333
MspI CCGG 1 cut(s) 573
MspR9I CCNGG 5 cut(s) 184, 651, 690, 738, 930
Mva1269I GAATGC 2 cut(s) 302, 1476
MvaI CCWGG 5 cut(s) 184, 651, 690, 738, 930
MvnI CGCG 1 cut(s) 629
MwoI GCNNNNNNNGC 3 cut(s) 92, 287, 1384
NcoI CCATGG 1 cut(s) 993
NdeII GATC 3 cut(s) 593, 1018, 1404
NlaIII CATG 2 cut(s) 997, 1430
NlaIV GGNNCC 5 cut(s) 25, 367, 595, 934, 1377
NmuCI GTSAC 1 cut(s) 1055
NsiI ATGCAT 3 cut(s) 1015, 1284, 1432
NspI RCATGY 1 cut(s) 1430
PacI TTAATTAA 1 cut(s) 1452
PceI AGGCCT 1 cut(s) 913
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 168, 544
PctI GAATGC 2 cut(s) 302, 1476
PdmI GAANNNNTTC 1 cut(s) 1210
PfeI GAWTC 3 cut(s) 698, 1001, 1504
PflMI CCANNNNNTGG 1 cut(s) 1202
PinAI ACCGGT 1 cut(s) 572
PkrI GCNGC 7 cut(s) 85, 101, 238, 334, 1144, 1335, 1475
PleI GAGTC 2 cut(s) 320, 454
PpsI GAGTC 2 cut(s) 320, 454
PshBI ATTAAT 2 cut(s) 1008, 1314
Psp6I CCWGG 5 cut(s) 182, 649, 688, 736, 928
PspGI CCWGG 5 cut(s) 182, 649, 688, 736, 928
PspN4I GGNNCC 5 cut(s) 25, 367, 595, 934, 1377
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 593
PvuII CAGCTG 1 cut(s) 1333
RsaI GTAC 5 cut(s) 261, 901, 1290, 1497, 1513
RsaNI GTAC 5 cut(s) 260, 900, 1289, 1496, 1512
SatI GCNGC 7 cut(s) 84, 100, 237, 333, 1143, 1334, 1474
Sau3AI GATC 3 cut(s) 593, 1018, 1404
SchI GAGTC 2 cut(s) 321, 455
ScrFI CCNGG 5 cut(s) 184, 651, 690, 738, 930
SduI GDGCHC 2 cut(s) 26, 736
SfaNI GCATC 3 cut(s) 1038, 1291, 1390
SgrAI CRCCGGYG 1 cut(s) 572
SmlI CTYRAG 1 cut(s) 1338
SmoI CTYRAG 1 cut(s) 1338
Sse9I AATT 8 cut(s) 205, 414, 549, 563, 682, 1170, 1179, 1449
SseBI AGGCCT 1 cut(s) 913
SsiI CCGC 7 cut(s) 81, 84, 93, 156, 333, 627, 1466
SspI AATATT 1 cut(s) 220
SspMI CTAG 1 cut(s) 410
StuI AGGCCT 1 cut(s) 913
StyD4I CCNGG 5 cut(s) 182, 649, 688, 736, 928
StyI CCWWGG 3 cut(s) 369, 716, 993
TaaI ACNGT 5 cut(s) 268, 349, 774, 1121, 1495
TaiI ACGT 1 cut(s) 755
TaqI TCGA 4 cut(s) 547, 1044, 1398, 1480
TaqII GACCGA 1 cut(s) 46
TasI AATT 8 cut(s) 205, 414, 549, 563, 682, 1170, 1179, 1449
TatI WGTACW 2 cut(s) 899, 1495
TauI GCSGC 2 cut(s) 86, 335
TfiI GAWTC 3 cut(s) 698, 1001, 1504
TscAI CASTG 5 cut(s) 708, 912, 1055, 1221, 1414
TseFI GTSAC 1 cut(s) 1055
TseI GCWGC 5 cut(s) 99, 236, 1142, 1333, 1473
Tsp45I GTSAC 1 cut(s) 1055
TspDTI ATGAA 4 cut(s) 503, 668, 1281, 1451
TspRI CASTG 5 cut(s) 708, 912, 1055, 1221, 1414
Van91I CCANNNNNTGG 1 cut(s) 1202
VspI ATTAAT 2 cut(s) 1008, 1314
XapI RAATTY 1 cut(s) 563
XceI RCATGY 1 cut(s) 1430
XcmI CCANNNNNNNNNTGG 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 60
XmnI GAANNNNTTC 1 cut(s) 1210
XspI CTAG 1 cut(s) 410
Zsp2I ATGCAT 3 cut(s) 1015, 1284, 1432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.