Rh5DG512600

Molybdate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014622
Physical Location & Seq
Forward (+)
79923356 .. 79924310
955 bp
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UTR
Exon/CDS
Intron
Rh5DG512600.1

Sequence Viewer

Length: 549 bp
ATGAACTTTGTTGGGTGCTGGATCGGGGCAATGCCGGTGTGCCATGGAGCTAGTGGACTAGTCGGGCAATACCGACTCGGGGGCCGGAGTGGCGCTTCTGTTGTGTTTTTGGGGATCGAGAAACTAGTTCTTGCTTGGGTGTTTTGGAATTCAGTTGTGAGTATTTTGAATCAGTTTCCAATTGGAATTCTTGGGGTGCTTTTGCTGTTTGCTGGGATTGAATTTGCTATGGCATCTAAGGACATGAATTCGAAAGAGGAGTCGTTTGTGATGTTGGTTTGTGTTGCGCTTTCGTTGACTGGGTCTAGCTCTGCGTTGGGGTTTGGGTGTGGGATTGTGCTGTTCTTGCTCCGAACCTGCTTCAACTCTGACGCCAACGACACCACGTTCAGGCGAAAGCTCTGCTTCTTCTCATTCAAATCTTTCAGCAATGGATCAAACTCATTTACTGAACTTGACGATGAAGAAGAAGCAGAGGAGCTTGGCCGAGATTTCTCCGAAATTGACATTATAAATGAAGCCGAGTTGGGCCAAACTTCAGTGGTTTAA

Protein Analysis

182

Amino Acids

19.7

Weight (kDa)

4.5

Isoelectric Point (pI)

33.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_MOT1 PF16983 1 - 75 9.6e-19 Molybdate transporter of MFS superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014622)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80310
prunus_persica Prupe.3G077200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0137081
rosa_laevigata RLG00000019606 RLG00000023044 RLG00000027400 RLG00000030801
rosa_multiflora Rmu_sc0001374.1_g000007
rosa_roxburghii Rroxscaffold_6G00407600
rosa_rugosa Rorug02G0337900
rosa_samantha Rh2AG387000 Rh2BG393800 Rh2CG374300 Rh2DG409900 Rh5DG512600 Rh6BG303900
rosa_wichuraiana Rw2G031670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 512
Acc36I ACCTGC 1 cut(s) 365
AclWI GGATC 3 cut(s) 29, 122, 442
AcoI YGGCCR 1 cut(s) 484
AcsI RAATTY 4 cut(s) 148, 186, 221, 247
AcuI CTGAAG 1 cut(s) 522
AcyI GRCGYC 1 cut(s) 372
AfiI CCNNNNNNNGG 2 cut(s) 79, 390
AgsI TTSAA 4 cut(s) 169, 221, 364, 418
AhlI ACTAGT 2 cut(s) 58, 124
AluBI AGCT 4 cut(s) 50, 309, 400, 481
AluI AGCT 4 cut(s) 50, 309, 400, 481
AlwI GGATC 3 cut(s) 29, 122, 442
Ama87I CYCGRG 1 cut(s) 77
AoxI GGCC 3 cut(s) 82, 484, 529
ApoI RAATTY 4 cut(s) 148, 186, 221, 247
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
AspLEI GCGC 2 cut(s) 95, 289
AspS9I GGNCC 2 cut(s) 82, 529
AsuII TTCGAA 1 cut(s) 251
AvaI CYCGRG 1 cut(s) 77
BcgI CGANNNNNNTGC 2 cut(s) 384, 418
BcuI ACTAGT 2 cut(s) 58, 124
BfaI CTAG 4 cut(s) 51, 59, 125, 306
BfoI RGCGCY 1 cut(s) 96
BfuAI ACCTGC 1 cut(s) 365
BglI GCCNNNNNGGC 1 cut(s) 90
BmeT110I CYCGRG 1 cut(s) 77
BmgT120I GGNCC 2 cut(s) 82, 529
BmiI GGNNCC 1 cut(s) 83
BmrI ACTGGG 1 cut(s) 309
BmsI GCATC 1 cut(s) 242
BmuI ACTGGG 1 cut(s) 309
Bpu14I TTCGAA 1 cut(s) 251
BsaHI GRCGYC 1 cut(s) 372
BsaJI CCNNGG 1 cut(s) 43
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 390
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 304
Bse3DI GCAATG 2 cut(s) 36, 436
BseDI CCNNGG 1 cut(s) 43
BseLI CCNNNNNNNGG 2 cut(s) 79, 390
BseMI GCAATG 2 cut(s) 36, 436
BseNI ACTGG 1 cut(s) 304
BseRI GAGGAG 2 cut(s) 272, 491
BseYI CCCAGC 1 cut(s) 212
BshFI GGCC 3 cut(s) 84, 486, 531
BsiHKCI CYCGRG 1 cut(s) 77
BsiSI CCGG 2 cut(s) 35, 85
BslI CCNNNNNNNGG 2 cut(s) 79, 390
BsnI GGCC 3 cut(s) 84, 486, 531
BsoBI CYCGRG 1 cut(s) 77
Bsp119I TTCGAA 1 cut(s) 251
Bsp143I GATC 3 cut(s) 21, 114, 434
Bsp19I CCATGG 1 cut(s) 43
BspANI GGCC 3 cut(s) 84, 486, 531
BspLI GGNNCC 1 cut(s) 83
BspMI ACCTGC 1 cut(s) 365
BspPI GGATC 3 cut(s) 29, 122, 442
BspT104I TTCGAA 1 cut(s) 251
BsrDI GCAATG 2 cut(s) 36, 436
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 3 cut(s) 21, 114, 434
BssNI GRCGYC 1 cut(s) 372
BssT1I CCWWGG 1 cut(s) 43
BstACI GRCGYC 1 cut(s) 372
BstBI TTCGAA 1 cut(s) 251
BstDEI CTNAG 1 cut(s) 237
BstDSI CCRYGG 1 cut(s) 43
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 95, 289
BstKTI GATC 3 cut(s) 24, 117, 437
BstMBI GATC 3 cut(s) 21, 114, 434
BstMWI GCNNNNNNNGC 2 cut(s) 90, 346
BsuRI GGCC 3 cut(s) 84, 486, 531
BtgI CCRYGG 1 cut(s) 43
BtsIMutI CAGTG 1 cut(s) 546
BveI ACCTGC 1 cut(s) 365
CfoI GCGC 2 cut(s) 95, 289
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 82, 529
CseI GACGC 1 cut(s) 380
CviAII CATG 2 cut(s) 44, 244
CviJI RGCY 8 cut(s) 50, 84, 309, 400, 481, 486, 521, 531
CviKI_1 RGCY 8 cut(s) 50, 84, 309, 400, 481, 486, 521, 531
DdeI CTNAG 1 cut(s) 237
DpnI GATC 3 cut(s) 23, 116, 436
DpnII GATC 3 cut(s) 21, 114, 434
EaeI YGGCCR 1 cut(s) 484
Eco130I CCWWGG 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 77
EcoRI GAATTC 3 cut(s) 148, 186, 247
EcoT14I CCWWGG 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 43
FaeI CATG 2 cut(s) 47, 247
FaiI YATR 4 cut(s) 45, 230, 245, 512
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FatI CATG 2 cut(s) 43, 243
FspBI CTAG 4 cut(s) 51, 59, 125, 306
GlaI GCGC 2 cut(s) 94, 288
GsaI CCCAGC 1 cut(s) 216
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 3 cut(s) 84, 486, 531
HapII CCGG 2 cut(s) 35, 85
HgaI GACGC 1 cut(s) 380
HhaI GCGC 2 cut(s) 95, 289
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 2 cut(s) 47, 247
Hin6I GCGC 2 cut(s) 93, 287
HinP1I GCGC 2 cut(s) 93, 287
HincII GTYRAC 1 cut(s) 297
HindII GTYRAC 1 cut(s) 297
HinfI GANTC 3 cut(s) 75, 169, 260
HpaII CCGG 2 cut(s) 35, 85
Hpy166II GTNNAC 2 cut(s) 56, 297
Hpy188I TCNGA 3 cut(s) 353, 370, 499
Hpy188III TCNNGA 1 cut(s) 118
Hpy8I GTNNAC 2 cut(s) 56, 297
HpyCH4IV ACGT 1 cut(s) 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 90, 346
HpyF3I CTNAG 1 cut(s) 237
HpySE526I ACGT 1 cut(s) 386
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 2 cut(s) 47, 247
HspAI GCGC 2 cut(s) 93, 287
Kzo9I GATC 3 cut(s) 21, 114, 434
LmnI GCTCC 3 cut(s) 47, 354, 478
LpnPI CCDG 7 cut(s) 4, 48, 98, 198, 285, 370, 376
LweI GCATC 1 cut(s) 242
MaeI CTAG 4 cut(s) 51, 59, 125, 306
MaeII ACGT 1 cut(s) 386
MalI GATC 3 cut(s) 23, 116, 436
MboI GATC 3 cut(s) 21, 114, 434
MboII GAAGA 3 cut(s) 400, 476, 479
MfeI CAATTG 1 cut(s) 180
MluCI AATT 6 cut(s) 148, 180, 186, 221, 247, 501
MlyI GAGTC 2 cut(s) 69, 269
MnlI CCTC 2 cut(s) 250, 469
MseI TTAA 1 cut(s) 547
MspI CCGG 2 cut(s) 35, 85
MunI CAATTG 1 cut(s) 180
MwoI GCNNNNNNNGC 2 cut(s) 90, 346
NcoI CCATGG 1 cut(s) 43
NdeII GATC 3 cut(s) 21, 114, 434
NlaIII CATG 2 cut(s) 47, 247
NlaIV GGNNCC 1 cut(s) 83
NmeAIII GCCGAG 2 cut(s) 512, 547
NspV TTCGAA 1 cut(s) 251
PfeI GAWTC 1 cut(s) 169
PflFI GACNNNGTC 1 cut(s) 301
PleI GAGTC 2 cut(s) 69, 268
PpsI GAGTC 2 cut(s) 69, 268
PsiI TTATAA 1 cut(s) 512
PspFI CCCAGC 1 cut(s) 212
PspN4I GGNNCC 1 cut(s) 83
PspPI GGNCC 2 cut(s) 82, 529
PsyI GACNNNGTC 1 cut(s) 301
SaqAI TTAA 1 cut(s) 547
Sau3AI GATC 3 cut(s) 21, 114, 434
Sau96I GGNCC 2 cut(s) 82, 529
SchI GAGTC 2 cut(s) 69, 269
SetI ASST 6 cut(s) 52, 311, 359, 389, 402, 483
SfaNI GCATC 1 cut(s) 242
SfuI TTCGAA 1 cut(s) 251
SpeI ACTAGT 2 cut(s) 58, 124
Sse9I AATT 6 cut(s) 148, 180, 186, 221, 247, 501
SspMI CTAG 4 cut(s) 51, 59, 125, 306
StyI CCWWGG 1 cut(s) 43
TaiI ACGT 1 cut(s) 389
TaqI TCGA 2 cut(s) 117, 251
TasI AATT 6 cut(s) 148, 180, 186, 221, 247, 501
TfiI GAWTC 1 cut(s) 169
Tru1I TTAA 1 cut(s) 547
Tru9I TTAA 1 cut(s) 547
TscAI CASTG 1 cut(s) 546
TspDTI ATGAA 4 cut(s) 17, 260, 477, 531
TspRI CASTG 1 cut(s) 546
Tth111I GACNNNGTC 1 cut(s) 301
XapI RAATTY 4 cut(s) 148, 186, 221, 247
XcmI CCANNNNNNNNNTGG 1 cut(s) 50
XspI CTAG 4 cut(s) 51, 59, 125, 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.