Rh6AG146500

5-kinase 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0020743
Physical Location & Seq
Reverse (-)
22959227 .. 22971753
12527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG146500.1

Sequence Viewer

Length: 1185 bp
ATGGTGACGGTGGGGGAAGGGTACCCTAATTTGGGACAACAGAATTTACTGGATATTGAAGTTAAATGCTGTCCTGAATTACCGCAGGCACTTACTGTTAGTAATACTGAGGTGGGGAAGGACCAAGATAATTTAGGGGGATCTGAGGATGTCAATCTGCATGTCTCCCCAATTTTTAGGCTTGAAATCAGCTTCCCGCCTATGCAGATTTCGAGCTTGACTGGGCTGACGTCAACTGTGGCTATGGAAAGGGAGGATGGGTCTGGGCTTAGACCTGTTCAGCCCACATCTAGCGAGCAAAAGGCTGTAATAGTAGGAAGTGTATTGTTGGGTGGGTTGGAGAATGGGCCAGGATGTGCTGACTCTAGTGAGGAGGTGGGTCAGCAGGCTGAGCACTTTTCTGAAACTGAGGGTTCTGGGGGTCGGACTGAGGCCGGGAAGACTTTTTGGGAGCTCGTTGGGGATGATGTGTCTGCAGCTATAAGGGTATTCATGGGTTCAGAGGAGATGTTGCGAAATGATGCTCTTAGGGAGCTTTCTTCTCCAGGGAAGAGGGGCAGCTTCTTTTACTTGACTCAGGATGATAGATTCATGATAAAGACTGTCAAAAAATCGGAAGTCAAGGTTTCCTCCTATTTATTGTGGAATTCCATTTGCATTCCACCGGAATGTGTTTTATCATTTGTCAACTTGACACTTGTTGATCTTCCTGGGCTTACAAAAGTTTTTGTTGAGGGTCAGCTCGATAGTATTATGCAGGACATTGAAAATATGGTTCGCTCCTACATTGAGAAGGATAGGAGGGATTTGACAGATATTGAAATATATGGGATGCAAGGAATCCCACCTGATGTCTTGGCTGCACATTATGGAGAGGAAGAATACGACGCCCCGTCAAAAATGGCTAAATGGAAATCCCATCAACTCAGCTTTTTGGTGGTGTGGTGCCAGGTTCACTGGGGATATATCCTCCCTAACCACGTTTTGAAATGCGGCCAATATACAATTCATCGGTCCCAATGCCTCATAACGGTTGGCAAGTTGCACCCCGTCCCCAGCCTTGGTATCCACAGCCTCCGGCAGTCTCTGGTCCTCCGCCTTCCGGATTGGGTTATTCACAACAGCCATTGTTTCCTGTGCAGCCACCTCTACCATCAACCCCCTGGACTTCAATCTTCACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04011 ko04070 ko04072 ko04139 ko04144 ko04666 ko04810 ko05231 map00562 map01100 map04011 map04070 map04072 map04139 map04144 map04666 map04810 map05231
Pfam Domains
Protein Families
MORN PIP5K

Protein Analysis

394

Amino Acids

43.63

Weight (kDa)

5.13

Isoelectric Point (pI)

54.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIP5K PF01504 172 - 211 9.9e-13 Phosphatidylinositol-4-phosphate 5-Kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020743)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004178.1_g000004
rosa_rugosa Rorug01G0064100
rosa_samantha Rh2CG593500 Rh6AG146500 Rh6DG259000
rosa_wichuraiana Rw3G029240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 233
Acc65I GGTACC 1 cut(s) 21
AccB1I GGYRCC 2 cut(s) 21, 945
AccIII TCCGGA 1 cut(s) 1102
AciI CCGC 4 cut(s) 83, 197, 993, 1096
AclWI GGATC 1 cut(s) 148
AcoI YGGCCR 1 cut(s) 994
AcsI RAATTY 2 cut(s) 43, 646
AcyI GRCGYC 2 cut(s) 230, 888
AfaI GTAC 1 cut(s) 23
AfiI CCNNNNNNNGG 5 cut(s) 31, 32, 1030, 1061, 1102
AgsI TTSAA 6 cut(s) 59, 185, 767, 821, 988, 1172
AhdI GACNNNNNGTC 1 cut(s) 892
AjnI CCWGG 5 cut(s) 349, 544, 709, 948, 1162
AjuI GAANNNNNNNTTGG 2 cut(s) 990, 1022
AluBI AGCT 8 cut(s) 192, 216, 454, 479, 535, 561, 742, 930
AluI AGCT 8 cut(s) 192, 216, 454, 479, 535, 561, 742, 930
Alw21I GWGCWC 2 cut(s) 396, 456
Alw26I GTCTC 2 cut(s) 169, 1089
AlwI GGATC 1 cut(s) 148
AlwNI CAGNNNCTG 1 cut(s) 1087
Aor13HI TCCGGA 1 cut(s) 1102
AoxI GGCC 3 cut(s) 347, 432, 994
ApeKI GCWGC 4 cut(s) 476, 558, 860, 1140
ApoI RAATTY 2 cut(s) 43, 646
Asp718I GGTACC 1 cut(s) 21
AspS9I GGNCC 4 cut(s) 121, 347, 1014, 1090
AsuC2I CCSGG 1 cut(s) 436
AsuHPI GGTGA 1 cut(s) 16
AvaII GGWCC 3 cut(s) 121, 1014, 1090
BanI GGYRCC 2 cut(s) 21, 945
BanII GRGCYC 1 cut(s) 456
BbsI GAAGAC 1 cut(s) 446
Bbv12I GWGCWC 2 cut(s) 396, 456
BbvI GCAGC 4 cut(s) 488, 570, 847, 1152
BccI CCATC 3 cut(s) 251, 927, 1161
BciT130I CCWGG 5 cut(s) 351, 546, 711, 950, 1164
BciVI GTATCC 1 cut(s) 1076
BcnI CCSGG 1 cut(s) 436
BcoDI GTCTC 2 cut(s) 169, 1089
BfaI CTAG 2 cut(s) 291, 366
BfmI CTRYAG 1 cut(s) 474
BfuI GTATCC 1 cut(s) 1076
BisI GCNGC 5 cut(s) 477, 559, 861, 994, 1141
BlpI GCTNAGC 1 cut(s) 390
BlsI GCNGC 5 cut(s) 478, 560, 862, 995, 1142
Bme1390I CCNGG 6 cut(s) 351, 436, 546, 711, 950, 1164
Bme18I GGWCC 3 cut(s) 121, 1014, 1090
BmeRI GACNNNNNGTC 1 cut(s) 892
BmgT120I GGNCC 4 cut(s) 121, 347, 1014, 1090
BmiI GGNNCC 3 cut(s) 23, 947, 1016
BmrFI CCNGG 6 cut(s) 351, 436, 546, 711, 950, 1164
BmrI ACTGGG 2 cut(s) 231, 967
BmsI GCATC 2 cut(s) 511, 822
BmuI ACTGGG 2 cut(s) 231, 967
BpiI GAAGAC 1 cut(s) 446
BpmI CTGGAG 1 cut(s) 528
Bpu1102I GCTNAGC 1 cut(s) 390
BpuMI CCSGG 1 cut(s) 436
BsaBI GATNNNNATC 1 cut(s) 153
BsaHI GRCGYC 2 cut(s) 230, 888
BsaJI CCNNGG 4 cut(s) 545, 710, 1060, 1162
BsaWI WCCGGW 2 cut(s) 664, 1102
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 32, 1030, 1061, 1102
Bse1I ACTGG 3 cut(s) 54, 226, 962
Bse8I GATNNNNATC 1 cut(s) 153
BseAI TCCGGA 1 cut(s) 1102
BseBI CCWGG 5 cut(s) 351, 546, 711, 950, 1164
BseDI CCNNGG 4 cut(s) 545, 710, 1060, 1162
BseGI GGATG 6 cut(s) 154, 262, 359, 469, 586, 837
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 5 cut(s) 31, 32, 1030, 1061, 1102
BseMII CTCAG 7 cut(s) 99, 135, 381, 399, 420, 590, 940
BseNI ACTGG 3 cut(s) 54, 226, 962
BseRI GAGGAG 2 cut(s) 386, 518
BseXI GCAGC 4 cut(s) 488, 570, 847, 1152
BseYI CCCAGC 1 cut(s) 1055
BsgI GTGCAG 2 cut(s) 846, 1159
BshFI GGCC 3 cut(s) 349, 434, 996
BshNI GGYRCC 2 cut(s) 21, 945
BsiHKAI GWGCWC 2 cut(s) 396, 456
BsiSI CCGG 4 cut(s) 435, 665, 1078, 1103
BslFI GGGAC 3 cut(s) 48, 1000, 1037
BslI CCNNNNNNNGG 5 cut(s) 31, 32, 1030, 1061, 1102
BsmAI GTCTC 2 cut(s) 169, 1089
BsmFI GGGAC 3 cut(s) 48, 1000, 1037
BsmI GAATGC 1 cut(s) 657
BsnI GGCC 3 cut(s) 349, 434, 996
Bsp1286I GDGCHC 2 cut(s) 396, 456
Bsp13I TCCGGA 1 cut(s) 1102
Bsp143I GATC 2 cut(s) 140, 703
Bsp1720I GCTNAGC 1 cut(s) 390
BspACI CCGC 4 cut(s) 83, 197, 993, 1096
BspANI GGCC 3 cut(s) 349, 434, 996
BspCNI CTCAG 7 cut(s) 100, 136, 382, 400, 421, 589, 939
BspEI TCCGGA 1 cut(s) 1102
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 3 cut(s) 23, 947, 1016
BspMAI CTGCAG 1 cut(s) 478
BspPI GGATC 1 cut(s) 148
BspT107I GGYRCC 2 cut(s) 21, 945
BsrI ACTGG 3 cut(s) 54, 226, 962
BssECI CCNNGG 4 cut(s) 545, 710, 1060, 1162
BssMI GATC 2 cut(s) 140, 703
BssNI GRCGYC 2 cut(s) 230, 888
BssT1I CCWWGG 1 cut(s) 1060
Bst2UI CCWGG 5 cut(s) 351, 546, 711, 950, 1164
Bst4CI ACNGT 6 cut(s) 10, 97, 238, 604, 1033, 1182
Bst6I CTCTTC 1 cut(s) 545
BstACI GRCGYC 2 cut(s) 230, 888
BstC8I GCNNGC 3 cut(s) 87, 296, 387
BstDEI CTNAG 9 cut(s) 108, 144, 269, 390, 408, 429, 527, 576, 926
BstF5I GGATG 6 cut(s) 154, 262, 359, 469, 586, 837
BstKTI GATC 2 cut(s) 143, 706
BstMAI GTCTC 2 cut(s) 169, 1089
BstMBI GATC 2 cut(s) 140, 703
BstMWI GCNNNNNNNGC 1 cut(s) 391
BstNI CCWGG 5 cut(s) 351, 546, 711, 950, 1164
BstNSI RCATGY 1 cut(s) 164
BstSCI CCNGG 6 cut(s) 349, 434, 544, 709, 948, 1162
BstSFI CTRYAG 1 cut(s) 474
BstV1I GCAGC 4 cut(s) 488, 570, 847, 1152
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 1 cut(s) 140
BstYI RGATCY 1 cut(s) 140
BsuI GTATCC 1 cut(s) 1076
BsuRI GGCC 3 cut(s) 349, 434, 996
BtsCI GGATG 6 cut(s) 154, 262, 359, 469, 586, 837
BtsIMutI CAGTG 1 cut(s) 955
Cac8I GCNNGC 3 cut(s) 87, 296, 387
CaiI CAGNNNCTG 1 cut(s) 1087
CciI TCATGA 1 cut(s) 591
Cfr13I GGNCC 4 cut(s) 121, 347, 1014, 1090
CseI GACGC 1 cut(s) 896
Csp6I GTAC 1 cut(s) 22
CviAII CATG 3 cut(s) 161, 493, 592
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 9 cut(s) 108, 144, 269, 390, 408, 429, 527, 576, 926
DpnI GATC 2 cut(s) 142, 705
DpnII GATC 2 cut(s) 140, 703
DriI GACNNNNNGTC 1 cut(s) 892
EaeI YGGCCR 1 cut(s) 994
Eam1104I CTCTTC 1 cut(s) 545
Eam1105I GACNNNNNGTC 1 cut(s) 892
EarI CTCTTC 1 cut(s) 545
EciI GGCGGA 1 cut(s) 1085
Ecl136II GAGCTC 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 1060
Eco24I GRGCYC 1 cut(s) 456
Eco47I GGWCC 3 cut(s) 121, 1014, 1090
Eco53kI GAGCTC 1 cut(s) 454
EcoICRI GAGCTC 1 cut(s) 454
EcoRI GAATTC 1 cut(s) 646
EcoRII CCWGG 5 cut(s) 349, 544, 709, 948, 1162
EcoT14I CCWWGG 1 cut(s) 1060
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 1060
FaeI CATG 3 cut(s) 164, 496, 595
FaqI GGGAC 3 cut(s) 48, 1000, 1037
FatI CATG 3 cut(s) 160, 492, 591
FauI CCCGC 1 cut(s) 204
Fnu4HI GCNGC 5 cut(s) 477, 559, 861, 994, 1141
FokI GGATG 6 cut(s) 161, 269, 366, 476, 593, 844
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 5 cut(s) 477, 559, 861, 994, 1141
FspBI CTAG 2 cut(s) 291, 366
GluI GCNGC 5 cut(s) 477, 559, 861, 994, 1141
GsaI CCCAGC 1 cut(s) 1059
GsuI CTGGAG 1 cut(s) 528
HaeIII GGCC 3 cut(s) 349, 434, 996
HapII CCGG 4 cut(s) 435, 665, 1078, 1103
HgaI GACGC 1 cut(s) 896
Hin1I GRCGYC 2 cut(s) 230, 888
Hin1II CATG 3 cut(s) 164, 496, 595
HincII GTYRAC 2 cut(s) 234, 688
HindII GTYRAC 2 cut(s) 234, 688
HinfI GANTC 4 cut(s) 362, 574, 588, 840
HpaII CCGG 4 cut(s) 435, 665, 1078, 1103
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 3 cut(s) 234, 688, 955
Hpy188I TCNGA 5 cut(s) 145, 403, 426, 502, 616
Hpy188III TCNNGA 4 cut(s) 74, 578, 592, 1103
Hpy8I GTNNAC 3 cut(s) 234, 688, 955
Hpy99I CGWCG 1 cut(s) 890
HpyAV CCTTC 4 cut(s) 11, 112, 787, 1109
HpyCH4III ACNGT 6 cut(s) 10, 97, 238, 604, 1033, 1182
HpyCH4IV ACGT 2 cut(s) 230, 981
HpyCH4V TGCA 9 cut(s) 160, 205, 476, 657, 757, 835, 863, 1045, 1140
HpyF10VI GCNNNNNNNGC 1 cut(s) 391
HpyF3I CTNAG 9 cut(s) 108, 144, 269, 390, 408, 429, 527, 576, 926
HpySE526I ACGT 2 cut(s) 230, 981
Hsp92I GRCGYC 2 cut(s) 230, 888
Hsp92II CATG 3 cut(s) 164, 496, 595
Kpn2I TCCGGA 1 cut(s) 1102
KpnI GGTACC 1 cut(s) 25
Kzo9I GATC 2 cut(s) 140, 703
LmnI GCTCC 3 cut(s) 451, 532, 785
Lsp1109I GCAGC 4 cut(s) 488, 570, 847, 1152
LweI GCATC 2 cut(s) 511, 822
MaeI CTAG 2 cut(s) 291, 366
MaeII ACGT 2 cut(s) 230, 981
MaeIII GTNAC 1 cut(s) 4
MalI GATC 2 cut(s) 142, 705
MboI GATC 2 cut(s) 140, 703
MboII GAAGA 6 cut(s) 451, 531, 562, 698, 890, 1167
MflI RGATCY 1 cut(s) 140
MhlI GDGCHC 2 cut(s) 396, 456
MluCI AATT 7 cut(s) 28, 43, 77, 130, 171, 646, 1005
MlyI GAGTC 2 cut(s) 356, 568
MmeI TCCRAC 2 cut(s) 318, 404
MroI TCCGGA 1 cut(s) 1102
MseI TTAA 1 cut(s) 63
MslI CAYNNNNRTG 1 cut(s) 667
MspI CCGG 4 cut(s) 435, 665, 1078, 1103
MspR9I CCNGG 6 cut(s) 351, 436, 546, 711, 950, 1164
Mva1269I GAATGC 1 cut(s) 657
MvaI CCWGG 5 cut(s) 351, 546, 711, 950, 1164
MwoI GCNNNNNNNGC 1 cut(s) 391
NciI CCSGG 1 cut(s) 436
NdeII GATC 2 cut(s) 140, 703
NlaIII CATG 3 cut(s) 164, 496, 595
NlaIV GGNNCC 3 cut(s) 23, 947, 1016
NmuCI GTSAC 1 cut(s) 4
NspI RCATGY 1 cut(s) 164
PagI TCATGA 1 cut(s) 591
PctI GAATGC 1 cut(s) 657
PfeI GAWTC 2 cut(s) 588, 840
PkrI GCNGC 5 cut(s) 478, 560, 862, 995, 1142
PleI GAGTC 2 cut(s) 356, 568
PpsI GAGTC 2 cut(s) 356, 568
Psp124BI GAGCTC 1 cut(s) 456
Psp6I CCWGG 5 cut(s) 349, 544, 709, 948, 1162
PspFI CCCAGC 1 cut(s) 1055
PspGI CCWGG 5 cut(s) 349, 544, 709, 948, 1162
PspN4I GGNNCC 3 cut(s) 23, 947, 1016
PspPI GGNCC 4 cut(s) 121, 347, 1014, 1090
PstI CTGCAG 1 cut(s) 478
PstNI CAGNNNCTG 1 cut(s) 1087
PsuI RGATCY 1 cut(s) 140
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
RseI CAYNNNNRTG 1 cut(s) 667
SacI GAGCTC 1 cut(s) 456
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 5 cut(s) 477, 559, 861, 994, 1141
Sau3AI GATC 2 cut(s) 140, 703
Sau96I GGNCC 4 cut(s) 121, 347, 1014, 1090
SchI GAGTC 2 cut(s) 356, 568
ScrFI CCNGG 6 cut(s) 351, 436, 546, 711, 950, 1164
SduI GDGCHC 2 cut(s) 396, 456
SfaNI GCATC 2 cut(s) 511, 822
SfcI CTRYAG 1 cut(s) 474
SinI GGWCC 3 cut(s) 121, 1014, 1090
SmiMI CAYNNNNRTG 1 cut(s) 667
Sse9I AATT 7 cut(s) 28, 43, 77, 130, 171, 646, 1005
SsiI CCGC 4 cut(s) 83, 197, 993, 1096
SspMI CTAG 2 cut(s) 291, 366
SstI GAGCTC 1 cut(s) 456
StyD4I CCNGG 6 cut(s) 349, 434, 544, 709, 948, 1162
StyI CCWWGG 1 cut(s) 1060
TaaI ACNGT 6 cut(s) 10, 97, 238, 604, 1033, 1182
TaiI ACGT 2 cut(s) 233, 984
TaqI TCGA 2 cut(s) 212, 744
TaqII GACCGA 1 cut(s) 1002
TasI AATT 7 cut(s) 28, 43, 77, 130, 171, 646, 1005
TauI GCSGC 1 cut(s) 996
TfiI GAWTC 2 cut(s) 588, 840
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 1 cut(s) 962
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 4 cut(s) 476, 558, 860, 1140
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 3 cut(s) 481, 580, 998
TspRI CASTG 1 cut(s) 962
VpaK11BI GGWCC 3 cut(s) 121, 1014, 1090
XapI RAATTY 2 cut(s) 43, 646
XceI RCATGY 1 cut(s) 164
XcmI CCANNNNNNNNNTGG 1 cut(s) 1160
XspI CTAG 2 cut(s) 291, 366
ZraI GACGTC 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.