Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0011521

Physical Location & Seq

Genomic Coordinates Reverse (-)

39569292 .. 39574155

Gene Structure 4864 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG219500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1311 bp

ATGGCGACCCTAGTCGCAATGCCCAGAATGAGACTCAAATTCACATCCTTCCCTCCATTTCTGGGCCAATCCTACAGGCCCAGAAGACTCTCACTCTTCAATGGCAGAAGGGCCTTTTCATTCTCTGCTTCTGCAACCCCAGACCAAGTACGTGTCCGCTTTGCTCCTTCTCCTACTGGGAATCTCCACGTTGGTGGAGCCAGAACTGCCCTCTTCAATTACCTCTTTGCCAGGTCCAATGGAGGCAAGTTTGTTTTGAGAATTGAAGACACTGATTTGGAGAGGTCTACTAAGGAATCTGAGGAGGCTGTGTTGCGGGATCTCTCTTGGCTTGGTCTTGATTGGGATGAAGGACCCGGTGTGGATGGAGATTATGGGCCATATCGGCAATCGGAAAGAAATTCTTTGTACAAGCAGTATGCTGAGAAGCTTTTAGAGTCTGGTTATGTCTATCGCTGCTTTTGCTCCAGTGAGGAACTAGAAAAAATGAAGGAGATTGCAAAGCTGAAGCAACTGCCTCCAGTATACTCAGGAAAGTGGGCCAGTGCAACTAATGAAGAAGTACAAGTAGAGCTGGAAAAAGGAACTCCTTACACATATAGGTTTCGTGTTCCAAAGGAAGGAAGTCTTAAGATCAATGATCTCATTCGAGGGCAGGTCAGTTGGAACTTGGACACTCTTGGAGATTTTGTAATTATGAGAAGCAATGGTCAACCGGTGTACAATTTTTGTGTCACAGTTGATGATGCTACCATGGCTATCTCACATGTTATTAGAGCAGAGGAGCATTTACCAAATACTCTAAGGCAAGCGTTAATATATAAGGCACTAGGATTTCCAATGCCTTCCTTTGCACATGTATCCTTAATTCTTGCACCTGATCGAAGTAAGTTATCAAAAAGGCATGGAGCAACTTCAGTGGGCCAGTTTAGGGAGATGGGCTATCTACCACAGGCAATGGTGAACTACTTGGCACTGTTAGGTTGGGGTGATGGCACTGAAAATGAATTTTTCACCTTGGAGCAGCTAGTTGAAAAATTTTCAATTGGTCGTGTCAACAAAAGTGGTGCCATTTTTGACAATACCAAGTTAAGGTGGATGAATGGTCAACACTTACGAGCACTTCCATCAGAGGAGTTGAAAAAACGTATTGGTGAGCAATGGAAAAGTAGCGGTATCCTTACAGAATCAGAGGGTTCATTTGTAGAAGTAAGACTCATCTCTTCATCACTTCCTTTTGTACGTTATTTAGTACATAACTTCCAGAAACATTGTCCATGTTCATTGCATTTAATAAGACAAATCATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006424 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006996 GO:0007005 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0035670 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0071704 GO:0071840 GO:0090304 GO:0090567 GO:0099402 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00860 ko00970 ko01100 ko01110 ko01120 map00860 map00970 map01100 map01110 map01120

Pfam Domains

Protein Families

tRNA-synt_1c

Protein Analysis

436

Amino Acids

49.53

Weight (kDa)

9.17

Isoelectric Point (pI)

42.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1c	PF00749	49 - 366	3.3e-104	tRNA synthetases class I (E and Q), catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011521)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g14910 FvH4_2g14910 FvH4_2g14910
malus_domestica	MD00G1184100.v1.1
prunus_persica	Prupe.4G159800_v2.0.a1
pyrus_communis	pycom11g23440
rosa_chinensis	RchiOBHm_Chr2g0162471 RchiOBHm_Chr5g0030101
rosa_multiflora	Rmu_sc0042269.1_g000001
rosa_roxburghii	Rroxscaffold_1G00049890 Rroxscaffold_1G00049930
rosa_rugosa	Rorug05G0117100 Rorug05G0117800
rosa_samantha	Rh5AG210100 Rh5BG209100 Rh5CG232900 Rh5DG213400 Rh6AG219500
rosa_wichuraiana	Rw5G019150 Rw5G019180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	646
AccB1I	GGYRCC	1 cut(s)	1067
AccI	GTMKAC	2 cut(s)	287, 525
AciI	CCGC	3 cut(s)	157, 316, 1173
AclWI	GGATC	1 cut(s)	327
AcsI	RAATTY	4 cut(s)	38, 400, 1007, 1037
AcuI	CTGAAG	2 cut(s)	527, 900
AfaI	GTAC	6 cut(s)	150, 410, 564, 722, 1242, 1254
AfiI	CCNNNNNNNGG	4 cut(s)	62, 620, 931, 1092
AflII	CTTAAG	1 cut(s)	629
AflIII	ACRYGT	3 cut(s)	151, 766, 856
AgeI	ACCGGT	1 cut(s)	715
AgsI	TTSAA	6 cut(s)	100, 217, 266, 1034, 1044, 1141
AjnI	CCWGG	1 cut(s)	230
AleI	CACNNNNGTG	1 cut(s)	192
AluBI	AGCT	4 cut(s)	430, 505, 574, 1027
AluI	AGCT	4 cut(s)	430, 505, 574, 1027
Alw21I	GWGCWC	1 cut(s)	1123
Alw26I	GTCTC	1 cut(s)	25
AlwI	GGATC	1 cut(s)	327
AoxI	GGCC	6 cut(s)	64, 77, 111, 377, 540, 922
ApeKI	GCWGC	2 cut(s)	456, 1024
ApoI	RAATTY	4 cut(s)	38, 400, 1007, 1037
AsiGI	ACCGGT	1 cut(s)	715
AspS9I	GGNCC	8 cut(s)	64, 78, 111, 234, 353, 377, 540, 922
AsuC2I	CCSGG	1 cut(s)	357
AsuHPI	GGTGA	4 cut(s)	973, 1001, 1006, 1166
AvaII	GGWCC	2 cut(s)	234, 353
BanI	GGYRCC	1 cut(s)	1067
BbsI	GAAGAC	2 cut(s)	91, 273
Bbv12I	GWGCWC	1 cut(s)	1123
BbvI	GCAGC	2 cut(s)	443, 1036
BccI	CCATC	4 cut(s)	359, 931, 986, 1135
BciT130I	CCWGG	1 cut(s)	232
BciVI	GTATCC	2 cut(s)	871, 1187
BcnI	CCSGG	1 cut(s)	357
BcoDI	GTCTC	1 cut(s)	25
BfaI	CTAG	4 cut(s)	11, 479, 830, 1028
BfmI	CTRYAG	1 cut(s)	73
BfrI	CTTAAG	1 cut(s)	629
BfuAI	ACCTGC	1 cut(s)	646
BfuI	GTATCC	2 cut(s)	871, 1187
BglI	GCCNNNNNGGC	1 cut(s)	385
BisI	GCNGC	2 cut(s)	457, 1025
BlsI	GCNGC	2 cut(s)	458, 1026
Bme1390I	CCNGG	2 cut(s)	232, 357
Bme18I	GGWCC	2 cut(s)	234, 353
BmgT120I	GGNCC	8 cut(s)	64, 78, 111, 234, 353, 377, 540, 922
BmiI	GGNNCC	3 cut(s)	199, 355, 1069
BmrFI	CCNGG	2 cut(s)	232, 357
BmrI	ACTGGG	1 cut(s)	186
BmsI	GCATC	1 cut(s)	736
BmuI	ACTGGG	1 cut(s)	186
BoxI	GACNNNNGTC	1 cut(s)	11
BpiI	GAAGAC	2 cut(s)	91, 273
BpmI	CTGGAG	2 cut(s)	451, 504
BpuMI	CCSGG	1 cut(s)	357
BsaAI	YACGTR	1 cut(s)	152
BsaJI	CCNNGG	2 cut(s)	753, 1017
BsaWI	WCCGGW	1 cut(s)	715
BsaXI	ACNNNNNCTCC	4 cut(s)	296, 326, 675, 705
Bsc4I	CCNNNNNNNGG	4 cut(s)	62, 620, 931, 1092
Bse118I	RCCGGY	1 cut(s)	715
Bse1I	ACTGG	5 cut(s)	181, 468, 521, 543, 925
Bse3DI	GCAATG	5 cut(s)	24, 712, 963, 1166, 1283
BseBI	CCWGG	1 cut(s)	232
BseDI	CCNNGG	2 cut(s)	753, 1017
BseGI	GGATG	4 cut(s)	44, 352, 370, 1104
BseLI	CCNNNNNNNGG	4 cut(s)	62, 620, 931, 1092
BseMI	GCAATG	5 cut(s)	24, 712, 963, 1166, 1283
BseMII	CTCAG	3 cut(s)	291, 414, 543
BseNI	ACTGG	5 cut(s)	181, 468, 521, 543, 925
BseRI	GAGGAG	3 cut(s)	317, 797, 1148
BseXI	GCAGC	2 cut(s)	443, 1036
BshFI	GGCC	6 cut(s)	66, 79, 113, 379, 542, 924
BshNI	GGYRCC	1 cut(s)	1067
BshTI	ACCGGT	1 cut(s)	715
BsiHKAI	GWGCWC	1 cut(s)	1123
BsiSI	CCGG	2 cut(s)	357, 716
BslI	CCNNNNNNNGG	4 cut(s)	62, 620, 931, 1092
BsmAI	GTCTC	1 cut(s)	25
BsnI	GGCC	6 cut(s)	66, 79, 113, 379, 542, 924
Bsp1286I	GDGCHC	1 cut(s)	1123
Bsp1407I	TGTACA	2 cut(s)	408, 720
Bsp143I	GATC	4 cut(s)	319, 633, 640, 880
Bsp19I	CCATGG	1 cut(s)	753
BspACI	CCGC	3 cut(s)	157, 316, 1173
BspANI	GGCC	6 cut(s)	66, 79, 113, 379, 542, 924
BspCNI	CTCAG	3 cut(s)	292, 415, 542
BspLI	GGNNCC	3 cut(s)	199, 355, 1069
BspMI	ACCTGC	1 cut(s)	646
BspPI	GGATC	1 cut(s)	327
BspT107I	GGYRCC	1 cut(s)	1067
BspTI	CTTAAG	1 cut(s)	629
BsrDI	GCAATG	5 cut(s)	24, 712, 963, 1166, 1283
BsrFI	RCCGGY	1 cut(s)	715
BsrGI	TGTACA	2 cut(s)	408, 720
BsrI	ACTGG	5 cut(s)	181, 468, 521, 543, 925
BssAI	RCCGGY	1 cut(s)	715
BssECI	CCNNGG	2 cut(s)	753, 1017
BssMI	GATC	4 cut(s)	319, 633, 640, 880
BssNAI	GTATAC	1 cut(s)	526
BssT1I	CCWWGG	2 cut(s)	753, 1017
Bst1107I	GTATAC	1 cut(s)	526
Bst2UI	CCWGG	1 cut(s)	232
Bst4CI	ACNGT	2 cut(s)	739, 978
Bst6I	CTCTTC	3 cut(s)	101, 218, 1228
BstAFI	CTTAAG	1 cut(s)	629
BstAUI	TGTACA	2 cut(s)	408, 720
BstBAI	YACGTR	1 cut(s)	152
BstC8I	GCNNGC	1 cut(s)	810
BstDEI	CTNAG	5 cut(s)	291, 300, 423, 529, 803
BstDSI	CCRYGG	1 cut(s)	753
BstF5I	GGATG	4 cut(s)	44, 352, 370, 1104
BstKTI	GATC	4 cut(s)	322, 636, 643, 883
BstMAI	GTCTC	1 cut(s)	25
BstMBI	GATC	4 cut(s)	319, 633, 640, 880
BstMWI	GCNNNNNNNGC	4 cut(s)	206, 385, 462, 755
BstNI	CCWGG	1 cut(s)	232
BstNSI	RCATGY	2 cut(s)	770, 860
BstPAI	GACNNNNGTC	1 cut(s)	11
BstSCI	CCNGG	2 cut(s)	230, 355
BstSFI	CTRYAG	1 cut(s)	73
BstV1I	GCAGC	2 cut(s)	443, 1036
BstV2I	GAAGAC	2 cut(s)	91, 273
BstX2I	RGATCY	1 cut(s)	319
BstXI	CCANNNNNNTGG	1 cut(s)	194
BstYI	RGATCY	1 cut(s)	319
BstZ17I	GTATAC	1 cut(s)	526
BsuI	GTATCC	2 cut(s)	871, 1187
BsuRI	GGCC	6 cut(s)	66, 79, 113, 379, 542, 924
BtgI	CCRYGG	1 cut(s)	753
BtsCI	GGATG	4 cut(s)	44, 352, 370, 1104
BtsIMutI	CAGTG	6 cut(s)	270, 475, 550, 924, 974, 996
BveI	ACCTGC	1 cut(s)	646
Cac8I	GCNNGC	1 cut(s)	810
Cfr10I	RCCGGY	1 cut(s)	715
Cfr13I	GGNCC	8 cut(s)	64, 78, 111, 234, 353, 377, 540, 922
Csp6I	GTAC	6 cut(s)	149, 409, 563, 721, 1241, 1253
CspAI	ACCGGT	1 cut(s)	715
CspCI	CAANNNNNGTGG	4 cut(s)	900, 935, 1045, 1080
CviAII	CATG	5 cut(s)	754, 767, 857, 905, 1278
CviQI	GTAC	6 cut(s)	149, 409, 563, 721, 1241, 1253
DdeI	CTNAG	5 cut(s)	291, 300, 423, 529, 803
DpnI	GATC	4 cut(s)	321, 635, 642, 882
DpnII	GATC	4 cut(s)	319, 633, 640, 880
Eam1104I	CTCTTC	3 cut(s)	101, 218, 1228
EarI	CTCTTC	3 cut(s)	101, 218, 1228
Eco130I	CCWWGG	2 cut(s)	753, 1017
Eco47I	GGWCC	2 cut(s)	234, 353
Eco57I	CTGAAG	2 cut(s)	527, 900
EcoO109I	RGGNCCY	2 cut(s)	111, 353
EcoRII	CCWGG	1 cut(s)	230
EcoT14I	CCWWGG	2 cut(s)	753, 1017
ErhI	CCWWGG	2 cut(s)	753, 1017
FaeI	CATG	5 cut(s)	757, 770, 860, 908, 1281
FalI	AAGNNNNNCTT	4 cut(s)	388, 420, 612, 644
FatI	CATG	5 cut(s)	753, 766, 856, 904, 1277
FauI	CCCGC	1 cut(s)	309
FblI	GTMKAC	2 cut(s)	287, 525
Fnu4HI	GCNGC	2 cut(s)	457, 1025
FokI	GGATG	4 cut(s)	31, 359, 377, 1111
Fsp4HI	GCNGC	2 cut(s)	457, 1025
FspBI	CTAG	4 cut(s)	11, 479, 830, 1028
GluI	GCNGC	2 cut(s)	457, 1025
GsuI	CTGGAG	2 cut(s)	451, 504
HaeIII	GGCC	6 cut(s)	66, 79, 113, 379, 542, 924
HapII	CCGG	2 cut(s)	357, 716
Hin1II	CATG	5 cut(s)	757, 770, 860, 908, 1281
HincII	GTYRAC	3 cut(s)	713, 1057, 1109
HindII	GTYRAC	3 cut(s)	713, 1057, 1109
HindIII	AAGCTT	1 cut(s)	428
HinfI	GANTC	7 cut(s)	33, 87, 181, 296, 437, 1187, 1215
HpaII	CCGG	2 cut(s)	357, 716
HphI	GGTGA	4 cut(s)	973, 1001, 1006, 1166
Hpy166II	GTNNAC	7 cut(s)	288, 526, 713, 721, 964, 1057, 1109
Hpy188I	TCNGA	4 cut(s)	301, 394, 1132, 1192
Hpy188III	TCNNGA	3 cut(s)	338, 531, 1264
Hpy8I	GTNNAC	7 cut(s)	288, 526, 713, 721, 964, 1057, 1109
HpyAV	CCTTC	7 cut(s)	58, 102, 177, 344, 484, 614, 855
HpyCH4III	ACNGT	2 cut(s)	739, 978
HpyCH4IV	ACGT	4 cut(s)	151, 189, 1147, 1243
HpyCH4V	TGCA	6 cut(s)	134, 500, 548, 854, 875, 1288
HpyF10VI	GCNNNNNNNGC	4 cut(s)	206, 385, 462, 755
HpyF3I	CTNAG	5 cut(s)	291, 300, 423, 529, 803
HpySE526I	ACGT	4 cut(s)	151, 189, 1147, 1243
Hsp92II	CATG	5 cut(s)	757, 770, 860, 908, 1281
Kzo9I	GATC	4 cut(s)	319, 633, 640, 880
LmnI	GCTCC	6 cut(s)	169, 197, 470, 784, 908, 1021
Lsp1109I	GCAGC	2 cut(s)	443, 1036
LweI	GCATC	1 cut(s)	736
MaeI	CTAG	4 cut(s)	11, 479, 830, 1028
MaeII	ACGT	4 cut(s)	151, 189, 1147, 1243
MaeIII	GTNAC	1 cut(s)	733
MalI	GATC	4 cut(s)	321, 635, 642, 882
MboI	GATC	4 cut(s)	319, 633, 640, 880
MboII	GAAGA	6 cut(s)	88, 96, 205, 278, 569, 1215
MfeI	CAATTG	1 cut(s)	1044
MflI	RGATCY	1 cut(s)	319
MhlI	GDGCHC	1 cut(s)	1123
MlyI	GAGTC	4 cut(s)	27, 81, 446, 1209
MmeI	TCCRAC	1 cut(s)	644
MseI	TTAA	5 cut(s)	630, 815, 866, 1091, 1292
MslI	CAYNNNNRTG	1 cut(s)	192
MspCI	CTTAAG	1 cut(s)	629
MspI	CCGG	2 cut(s)	357, 716
MspR9I	CCNGG	2 cut(s)	232, 357
MunI	CAATTG	1 cut(s)	1044
MvaI	CCWGG	1 cut(s)	232
MwoI	GCNNNNNNNGC	4 cut(s)	206, 385, 462, 755
NciI	CCSGG	1 cut(s)	357
NcoI	CCATGG	1 cut(s)	753
NdeII	GATC	4 cut(s)	319, 633, 640, 880
NlaIII	CATG	5 cut(s)	757, 770, 860, 908, 1281
NlaIV	GGNNCC	3 cut(s)	199, 355, 1069
NmuCI	GTSAC	1 cut(s)	733
NspI	RCATGY	2 cut(s)	770, 860
OliI	CACNNNNGTG	1 cut(s)	192
PciI	ACATGT	2 cut(s)	766, 856
PfeI	GAWTC	3 cut(s)	181, 296, 1187
PinAI	ACCGGT	1 cut(s)	715
PkrI	GCNGC	2 cut(s)	458, 1026
PleI	GAGTC	4 cut(s)	27, 81, 445, 1209
PpsI	GAGTC	4 cut(s)	27, 81, 445, 1209
Ppu21I	YACGTR	1 cut(s)	152
PpuMI	RGGWCCY	1 cut(s)	353
PscI	ACATGT	2 cut(s)	766, 856
PshAI	GACNNNNGTC	1 cut(s)	11
Psp5II	RGGWCCY	1 cut(s)	353
Psp6I	CCWGG	1 cut(s)	230
PspGI	CCWGG	1 cut(s)	230
PspN4I	GGNNCC	3 cut(s)	199, 355, 1069
PspPI	GGNCC	8 cut(s)	64, 78, 111, 234, 353, 377, 540, 922
PspPPI	RGGWCCY	1 cut(s)	353
PsuI	RGATCY	1 cut(s)	319
RsaI	GTAC	6 cut(s)	150, 410, 564, 722, 1242, 1254
RsaNI	GTAC	6 cut(s)	149, 409, 563, 721, 1241, 1253
RseI	CAYNNNNRTG	1 cut(s)	192
SaqAI	TTAA	5 cut(s)	630, 815, 866, 1091, 1292
SatI	GCNGC	2 cut(s)	457, 1025
Sau3AI	GATC	4 cut(s)	319, 633, 640, 880
Sau96I	GGNCC	8 cut(s)	64, 78, 111, 234, 353, 377, 540, 922
SchI	GAGTC	4 cut(s)	27, 81, 446, 1209
ScrFI	CCNGG	2 cut(s)	232, 357
SduI	GDGCHC	1 cut(s)	1123
SfaNI	GCATC	1 cut(s)	736
SfcI	CTRYAG	1 cut(s)	73
SinI	GGWCC	2 cut(s)	234, 353
SmiMI	CAYNNNNRTG	1 cut(s)	192
SmlI	CTYRAG	1 cut(s)	629
SmoI	CTYRAG	1 cut(s)	629
SsiI	CCGC	3 cut(s)	157, 316, 1173
SspMI	CTAG	4 cut(s)	11, 479, 830, 1028
StyD4I	CCNGG	2 cut(s)	230, 355
StyI	CCWWGG	2 cut(s)	753, 1017
TaaI	ACNGT	2 cut(s)	739, 978
TaiI	ACGT	4 cut(s)	154, 192, 1150, 1246
TaqI	TCGA	2 cut(s)	649, 883
TatI	WGTACW	4 cut(s)	408, 562, 720, 1252
TfiI	GAWTC	3 cut(s)	181, 296, 1187
Tru1I	TTAA	5 cut(s)	630, 815, 866, 1091, 1292
Tru9I	TTAA	5 cut(s)	630, 815, 866, 1091, 1292
TscAI	CASTG	6 cut(s)	277, 475, 550, 924, 981, 1003
TseFI	GTSAC	1 cut(s)	733
TseI	GCWGC	2 cut(s)	456, 1024
Tsp45I	GTSAC	1 cut(s)	733
TspDTI	ATGAA	9 cut(s)	108, 363, 503, 570, 1020, 1115, 1188, 1215, 1272
TspRI	CASTG	6 cut(s)	277, 475, 550, 924, 981, 1003
Vha464I	CTTAAG	1 cut(s)	629
VpaK11BI	GGWCC	2 cut(s)	234, 353
XapI	RAATTY	4 cut(s)	38, 400, 1007, 1037
XceI	RCATGY	2 cut(s)	770, 860
XmiI	GTMKAC	2 cut(s)	287, 525
XspI	CTAG	4 cut(s)	11, 479, 830, 1028

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.