Rh6CG053500

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0019842
Physical Location & Seq
Forward (+)
5542000 .. 5543763
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG053500.1

Sequence Viewer

Length: 780 bp
ATGGCCACTTCTCTACTTTGTCCAAACACCTTCCTTCATGGCACCAAGATCTTCTCTGCTCCCCCAAGAAGGGTCTCGCCTCCAAGAAGCTTCAAACCCGTAGCTTCCACGGCTTCGGACCACGTTCCCAAGCAGTTCAGGGAAGAGAATCTCAAAGATGGATTGATGGATAATTACAAGAATGTGCCGCAATATCTGTATGGGCTTTCTCCTTCCCAAATGGACATGTTTATGACTGAAGATAACCCTGCAAGACGCCAGTCGGAAAGTGTTACAGAGAATAGCATTTCATCCGCCAACAACTATTTGAACAATGGAGGGATGTGGAGTATGTCAGGCATGGATGAAAAGGGCCCTGCAAAATATAGTATGAGTGTGAGCATGTACCGTGGTGGTGCAAGAGGATACGGAAGACCCCGAACTGCTCCTCCGGATTTGCCTTCTTTGCTATTGGATGCTCGGATTTGCTACTTGGGAATGCCGATTGTGCCAGCAGTAACTGAGCTTCTGGTTGCTCAGTTTATGTGGTTGGATTATGATAACCCCTCGAAGCCTATATATCTGTACATAAATTCATCTGGAACACAGAATGAGAAGATGGAGAGCGTTGGATCTGAAACTGAGGCATATGCCATTGCTGACATGATGAAATATGTCAAAGCAGACGTGTATACAGTGAATTGTGGCATGGCCTATGGTCAAGCAGCACTGCTTCTGTCACTTGGAACAAAGGGTTATCGTGCTGTGCAGCCGAATTCCTCCAGTATGATCCCCTCTTGA

Protein Analysis

259

Amino Acids

28.54

Weight (kDa)

7.63

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 147 - 257 3.7e-22 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019842)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0339141
rosa_roxburghii Rroxscaffold_5G00370800
rosa_samantha Rh6CG051500 Rh6CG052100 Rh6CG053500 Rh6DG049100 Rh6DG049400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 41
AccI GTMKAC 1 cut(s) 671
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 2 cut(s) 188, 294
AclWI GGATC 2 cut(s) 619, 763
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 571, 754
AcuI CTGAAG 1 cut(s) 258
AcyI GRCGYC 1 cut(s) 256
AfaI GTAC 2 cut(s) 386, 566
AfiI CCNNNNNNNGG 2 cut(s) 69, 70
AflIII ACRYGT 2 cut(s) 225, 666
AgsI TTSAA 2 cut(s) 94, 310
AjiI CACGTC 1 cut(s) 667
AloI GAACNNNNNNTCC 2 cut(s) 412, 444
AluBI AGCT 3 cut(s) 90, 104, 505
AluI AGCT 3 cut(s) 90, 104, 505
Alw26I GTCTC 1 cut(s) 79
AlwI GGATC 2 cut(s) 619, 763
AlwNI CAGNNNCTG 1 cut(s) 500
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 3 cut(s) 3, 352, 690
ApaI GGGCCC 1 cut(s) 356
ApeKI GCWGC 2 cut(s) 704, 748
ApoI RAATTY 2 cut(s) 571, 754
AspS9I GGNCC 3 cut(s) 118, 352, 353
AvaII GGWCC 1 cut(s) 118
BaeGI GKGCMC 1 cut(s) 356
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 1 cut(s) 356
BarI GAAGNNNNNNTAC 2 cut(s) 489, 521
BbsI GAAGAC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 716, 760
BccI CCATC 3 cut(s) 152, 160, 592
BceAI ACGGC 1 cut(s) 126
BciVI GTATCC 1 cut(s) 398
BcoDI GTCTC 1 cut(s) 79
BfuI GTATCC 1 cut(s) 398
BglII AGATCT 1 cut(s) 48
BisI GCNGC 3 cut(s) 188, 705, 749
BlsI GCNGC 3 cut(s) 189, 706, 750
Bme18I GGWCC 1 cut(s) 118
BmgBI CACGTC 1 cut(s) 667
BmgT120I GGNCC 3 cut(s) 118, 352, 353
BmiI GGNNCC 2 cut(s) 43, 354
BmsI GCATC 1 cut(s) 445
BoxI GACNNNNGTC 1 cut(s) 259
BpiI GAAGAC 1 cut(s) 418
BpmI CTGGAG 1 cut(s) 745
BsaHI GRCGYC 1 cut(s) 256
BsaI GGTCTC 1 cut(s) 79
BsaJI CCNNGG 2 cut(s) 108, 388
BsaWI WCCGGW 1 cut(s) 430
BsaXI ACNNNNNCTCC 2 cut(s) 412, 442
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 70
Bse1I ACTGG 2 cut(s) 259, 762
Bse3DI GCAATG 1 cut(s) 633
BseAI TCCGGA 1 cut(s) 430
BseDI CCNNGG 2 cut(s) 108, 388
BseGI GGATG 4 cut(s) 290, 327, 349, 460
BseLI CCNNNNNNNGG 2 cut(s) 69, 70
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 3 cut(s) 492, 530, 612
BseNI ACTGG 2 cut(s) 259, 762
BseRI GAGGAG 1 cut(s) 417
BseSI GKGCMC 1 cut(s) 356
BseXI GCAGC 2 cut(s) 716, 760
BsgI GTGCAG 1 cut(s) 767
BshFI GGCC 3 cut(s) 5, 354, 692
BshNI GGYRCC 1 cut(s) 41
BsiSI CCGG 1 cut(s) 431
BslI CCNNNNNNNGG 2 cut(s) 69, 70
BsmAI GTCTC 1 cut(s) 79
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 3 cut(s) 5, 354, 692
Bso31I GGTCTC 1 cut(s) 79
Bsp120I GGGCCC 1 cut(s) 352
Bsp1286I GDGCHC 1 cut(s) 356
Bsp13I TCCGGA 1 cut(s) 430
Bsp1407I TGTACA 1 cut(s) 564
Bsp143I GATC 3 cut(s) 48, 611, 768
BspACI CCGC 2 cut(s) 188, 294
BspANI GGCC 3 cut(s) 5, 354, 692
BspCNI CTCAG 3 cut(s) 493, 529, 613
BspEI TCCGGA 1 cut(s) 430
BspLI GGNNCC 2 cut(s) 43, 354
BspPI GGATC 2 cut(s) 619, 763
BspT107I GGYRCC 1 cut(s) 41
BspTNI GGTCTC 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 633
BsrGI TGTACA 1 cut(s) 564
BsrI ACTGG 2 cut(s) 259, 762
BssECI CCNNGG 2 cut(s) 108, 388
BssMI GATC 3 cut(s) 48, 611, 768
BssNAI GTATAC 1 cut(s) 672
BssNI GRCGYC 1 cut(s) 256
Bst1107I GTATAC 1 cut(s) 672
Bst4CI ACNGT 2 cut(s) 389, 676
Bst6I CTCTTC 1 cut(s) 138
BstACI GRCGYC 1 cut(s) 256
BstAUI TGTACA 1 cut(s) 564
BstC8I GCNNGC 1 cut(s) 492
BstDEI CTNAG 3 cut(s) 501, 516, 621
BstDSI CCRYGG 2 cut(s) 108, 388
BstF5I GGATG 4 cut(s) 290, 327, 349, 460
BstKTI GATC 3 cut(s) 51, 614, 771
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 3 cut(s) 48, 611, 768
BstMWI GCNNNNNNNGC 3 cut(s) 110, 445, 487
BstNSI RCATGY 2 cut(s) 229, 385
BstPAI GACNNNNGTC 1 cut(s) 259
BstSLI GKGCMC 1 cut(s) 356
BstV1I GCAGC 2 cut(s) 716, 760
BstV2I GAAGAC 1 cut(s) 418
BstX2I RGATCY 2 cut(s) 48, 611
BstYI RGATCY 2 cut(s) 48, 611
BstZ17I GTATAC 1 cut(s) 672
BsuI GTATCC 1 cut(s) 398
BsuRI GGCC 3 cut(s) 5, 354, 692
BtgI CCRYGG 2 cut(s) 108, 388
BtrI CACGTC 1 cut(s) 667
BtsCI GGATG 4 cut(s) 290, 327, 349, 460
BtsI GCAGTG 1 cut(s) 707
BtsIMutI CAGTG 2 cut(s) 681, 707
Cac8I GCNNGC 1 cut(s) 492
CaiI CAGNNNCTG 1 cut(s) 500
Cfr13I GGNCC 3 cut(s) 118, 352, 353
CseI GACGC 1 cut(s) 264
Csp6I GTAC 2 cut(s) 385, 565
CviAII CATG 6 cut(s) 38, 226, 340, 382, 643, 688
CviQI GTAC 2 cut(s) 385, 565
DdeI CTNAG 3 cut(s) 501, 516, 621
DpnI GATC 3 cut(s) 50, 613, 770
DpnII GATC 3 cut(s) 48, 611, 768
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
EciI GGCGGA 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 356
Eco31I GGTCTC 1 cut(s) 79
Eco47I GGWCC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 258
EcoO109I RGGNCCY 2 cut(s) 352, 353
EcoRI GAATTC 1 cut(s) 754
EcoT38I GRGCYC 1 cut(s) 356
FaeI CATG 6 cut(s) 41, 229, 343, 385, 646, 691
FatI CATG 6 cut(s) 37, 225, 339, 381, 642, 687
FauNDI CATATG 1 cut(s) 628
FblI GTMKAC 1 cut(s) 671
Fnu4HI GCNGC 3 cut(s) 188, 705, 749
FokI GGATG 4 cut(s) 277, 334, 356, 467
FriOI GRGCYC 1 cut(s) 356
Fsp4HI GCNGC 3 cut(s) 188, 705, 749
GluI GCNGC 3 cut(s) 188, 705, 749
GsuI CTGGAG 1 cut(s) 745
HaeIII GGCC 3 cut(s) 5, 354, 692
HapII CCGG 1 cut(s) 431
HgaI GACGC 1 cut(s) 264
Hin1I GRCGYC 1 cut(s) 256
Hin1II CATG 6 cut(s) 41, 229, 343, 385, 646, 691
HindIII AAGCTT 1 cut(s) 88
HinfI GANTC 1 cut(s) 148
HpaII CCGG 1 cut(s) 431
Hpy166II GTNNAC 1 cut(s) 672
Hpy188I TCNGA 4 cut(s) 118, 265, 462, 616
Hpy188III TCNNGA 3 cut(s) 431, 579, 777
Hpy8I GTNNAC 1 cut(s) 672
HpyAV CCTTC 5 cut(s) 40, 44, 63, 222, 450
HpyCH4III ACNGT 2 cut(s) 389, 676
HpyCH4IV ACGT 2 cut(s) 123, 666
HpyCH4V TGCA 4 cut(s) 251, 359, 398, 748
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 445, 487
HpyF3I CTNAG 3 cut(s) 501, 516, 621
HpySE526I ACGT 2 cut(s) 123, 666
Hsp92I GRCGYC 1 cut(s) 256
Hsp92II CATG 6 cut(s) 41, 229, 343, 385, 646, 691
Kpn2I TCCGGA 1 cut(s) 430
Kzo9I GATC 3 cut(s) 48, 611, 768
LmnI GCTCC 2 cut(s) 64, 430
Lsp1109I GCAGC 2 cut(s) 716, 760
LweI GCATC 1 cut(s) 445
MaeII ACGT 2 cut(s) 123, 666
MaeIII GTNAC 3 cut(s) 271, 496, 717
MalI GATC 3 cut(s) 50, 613, 770
MboI GATC 3 cut(s) 48, 611, 768
MboII GAAGA 5 cut(s) 43, 155, 251, 423, 607
MflI RGATCY 2 cut(s) 48, 611
MhlI GDGCHC 1 cut(s) 356
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 172, 571, 679, 754
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 243, 510, 589
MnlI CCTC 7 cut(s) 90, 311, 395, 438, 556, 616, 769
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 430
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 230
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 431
Mva1269I GAATGC 1 cut(s) 483
MwoI GCNNNNNNNGC 3 cut(s) 110, 445, 487
NdeI CATATG 1 cut(s) 628
NdeII GATC 3 cut(s) 48, 611, 768
NlaIII CATG 6 cut(s) 41, 229, 343, 385, 646, 691
NlaIV GGNNCC 2 cut(s) 43, 354
NmuCI GTSAC 1 cut(s) 717
NspI RCATGY 2 cut(s) 229, 385
PciI ACATGT 1 cut(s) 225
PctI GAATGC 1 cut(s) 483
PfeI GAWTC 1 cut(s) 148
PkrI GCNGC 3 cut(s) 189, 706, 750
PscI ACATGT 1 cut(s) 225
PshAI GACNNNNGTC 1 cut(s) 259
PspN4I GGNNCC 2 cut(s) 43, 354
PspOMI GGGCCC 1 cut(s) 352
PspPI GGNCC 3 cut(s) 118, 352, 353
PstNI CAGNNNCTG 1 cut(s) 500
PsuI RGATCY 2 cut(s) 48, 611
RsaI GTAC 2 cut(s) 386, 566
RsaNI GTAC 2 cut(s) 385, 565
RseI CAYNNNNRTG 1 cut(s) 230
SatI GCNGC 3 cut(s) 188, 705, 749
Sau3AI GATC 3 cut(s) 48, 611, 768
Sau96I GGNCC 3 cut(s) 118, 352, 353
SduI GDGCHC 1 cut(s) 356
SetI ASST 6 cut(s) 32, 92, 106, 126, 507, 669
SfaNI GCATC 1 cut(s) 445
SinI GGWCC 1 cut(s) 118
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 4 cut(s) 172, 571, 679, 754
SsiI CCGC 2 cut(s) 188, 294
TaaI ACNGT 2 cut(s) 389, 676
TaiI ACGT 2 cut(s) 126, 669
TaqI TCGA 1 cut(s) 548
TasI AATT 4 cut(s) 172, 571, 679, 754
TatI WGTACW 1 cut(s) 564
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 1 cut(s) 148
TscAI CASTG 2 cut(s) 681, 714
TseFI GTSAC 1 cut(s) 717
TseI GCWGC 2 cut(s) 704, 748
Tsp45I GTSAC 1 cut(s) 717
TspDTI ATGAA 5 cut(s) 26, 279, 360, 564, 662
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 2 cut(s) 681, 714
VpaK11BI GGWCC 1 cut(s) 118
XapI RAATTY 2 cut(s) 571, 754
XceI RCATGY 2 cut(s) 229, 385
XmiI GTMKAC 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.