Rh6CG330700

Histone H2A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0019962
Physical Location & Seq
Forward (+)
51782481 .. 51789628
7148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG330700.1

Sequence Viewer

Length: 672 bp
ATGGGCCCTCAAAAGCCCACACCCTCATGTCTGCATGGTCTTCTCTTCCTCCGCCGACGGACCCAGATGGAGCCTGTGACACAATTTCAACGGCAACAAGAACGGCGCCGCAAGAAACCCAGAAACACAGAAGAGGCGGAGGAAGGAGAAAGAGGAGAAGAGACAAGGGTGAGTTGTGAAGTGGATATGATTTCGTGTTCCTCCTACGACGACTCCCCCCCTTCTCTCTCCCGACTTCTCAAGAACCCTTCAGCAGGATTAATGGCGGGGAAAGGCGGAAAGGGGCTTTTGGCTCAGAAGACCACAGCAGCGAGCAAGGACAAGGACAAGGATAAGAAGAGGCCTGTTTCGCGCTCGTCTCGTGCTGGAATTCAGTTTCCGGTTGGTAGGATTCATAGGCAGCTGAAACAAAGGATTGCTGCCCATGGGCGTGTTGGGGCTACTGCTGCTGTCTACTTGGCCTCAATCCTCGAGTACCTGACTGCTGAAGTTCTTGAGTTGGCTGGAAATGCTAGCAAAGATCTCAAGGTGAAGAGAATTACCCCAAGGCATCTTCAGCTGGCTATTAGGGGAGATGAGGAGCTTGACACCCTGATTAAAGGGACCATTGCTGGAGGTGGAGTCATTCCTCACATCCACAAGTCCCTCATCAACAAGACCTCGAAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006325 GO:0006342 GO:0006355 GO:0006996 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0040029 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071840 GO:0080090 GO:0097159 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04014 ko04015 ko04016 ko04020 ko04022 ko04024 ko04070 ko04114 ko04217 ko04218 ko04261 ko04270 ko04371 ko04626 ko04713 ko04720 ko04722 ko04728 ko04740 ko04744 ko04745 ko04750 ko04910 ko04912 ko04915 ko04916 ko04921 ko04922 ko04924 ko04925 ko04970 ko04971 ko05010 ko05031 ko05034 ko05133 ko05152 ko05167 ko05200 ko05214 ko05322 ko05418 map04014 map04015 map04016 map04020 map04022 map04024 map04070 map04114 map04217 map04218 map04261 map04270 map04371 map04626 map04713 map04720 map04722 map04728 map04740 map04744 map04745 map04750 map04910 map04912 map04915 map04916 map04921 map04922 map04924 map04925 map04970 map04971 map05010 map05031 map05034 map05133 map05152 map05167 map05200 map05214 map05322 map05418
Pfam Domains
Protein Families
EF-hand_7 Histone Histone_H2A_C

Protein Analysis

223

Amino Acids

24.58

Weight (kDa)

10.04

Isoelectric Point (pI)

55.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 106 - 191 6e-21 Core histone H2A/H2B/H3/H4 domain
Histone_H2A_C PF16211 193 - 222 3.3e-10 C-terminus of histone H2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019962)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0242481
rosa_samantha Rh1CG079500 Rh1DG085700 Rh4AG131000 Rh6AG454400 Rh6CG330700 Rh7BG440900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 453
AccII CGCG 1 cut(s) 352
AciI CCGC 5 cut(s) 52, 109, 137, 266, 276
AcsI RAATTY 1 cut(s) 369
AcuI CTGAAG 3 cut(s) 234, 507, 539
AcyI GRCGYC 1 cut(s) 106
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 1 cut(s) 254
AgsI TTSAA 1 cut(s) 89
AluBI AGCT 3 cut(s) 403, 559, 583
AluI AGCT 3 cut(s) 403, 559, 583
Alw26I GTCTC 2 cut(s) 155, 363
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 3 cut(s) 4, 341, 459
ApaI GGGCCC 1 cut(s) 8
ApeKI GCWGC 4 cut(s) 308, 400, 419, 446
ApoI RAATTY 1 cut(s) 369
AseI ATTAAT 1 cut(s) 260
AspLEI GCGC 2 cut(s) 108, 354
AspS9I GGNCC 4 cut(s) 4, 5, 60, 603
AsuHPI GGTGA 2 cut(s) 181, 541
AsuNHI GCTAGC 1 cut(s) 512
AvaI CYCGRG 1 cut(s) 470
AvaII GGWCC 2 cut(s) 60, 603
BaeGI GKGCMC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 105
BanII GRGCYC 1 cut(s) 8
BauI CACGAG 1 cut(s) 360
BbsI GAAGAC 2 cut(s) 32, 305
BbvI GCAGC 4 cut(s) 320, 406, 412, 433
BccI CCATC 1 cut(s) 61
BceAI ACGGC 2 cut(s) 107, 119
BcoDI GTCTC 2 cut(s) 155, 363
BfaI CTAG 1 cut(s) 513
BfoI RGCGCY 1 cut(s) 109
BglII AGATCT 1 cut(s) 520
BisI GCNGC 5 cut(s) 109, 309, 401, 420, 447
BlsI GCNGC 5 cut(s) 110, 310, 402, 421, 448
Bme18I GGWCC 2 cut(s) 60, 603
BmeT110I CYCGRG 1 cut(s) 470
BmgT120I GGNCC 4 cut(s) 4, 5, 60, 603
BmiI GGNNCC 5 cut(s) 6, 62, 72, 107, 604
BmsI GCATC 1 cut(s) 559
BmtI GCTAGC 1 cut(s) 516
BpiI GAAGAC 2 cut(s) 32, 305
BpmI CTGGAG 1 cut(s) 633
BpuEI CTTGAG 3 cut(s) 224, 509, 515
BsaHI GRCGYC 1 cut(s) 106
BsaJI CCNNGG 2 cut(s) 424, 545
BsaWI WCCGGW 1 cut(s) 379
BsaXI ACNNNNNCTCC 4 cut(s) 197, 227, 606, 636
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 606
BseDI CCNNGG 2 cut(s) 424, 545
BseGI GGATG 1 cut(s) 633
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMI GCAATG 1 cut(s) 606
BseMII CTCAG 1 cut(s) 308
BseRI GAGGAG 2 cut(s) 168, 593
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 4 cut(s) 320, 406, 412, 433
Bsh1236I CGCG 1 cut(s) 352
BshFI GGCC 3 cut(s) 6, 343, 461
BshNI GGYRCC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 1 cut(s) 380
BslFI GGGAC 2 cut(s) 616, 628
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 2 cut(s) 155, 363
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 2 cut(s) 616, 628
BsnI GGCC 3 cut(s) 6, 343, 461
BsoBI CYCGRG 1 cut(s) 470
Bsp120I GGGCCC 1 cut(s) 4
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 1 cut(s) 520
Bsp19I CCATGG 1 cut(s) 424
BspACI CCGC 5 cut(s) 52, 109, 137, 266, 276
BspANI GGCC 3 cut(s) 6, 343, 461
BspCNI CTCAG 1 cut(s) 307
BspFNI CGCG 1 cut(s) 352
BspLI GGNNCC 5 cut(s) 6, 62, 72, 107, 604
BspOI GCTAGC 1 cut(s) 516
BspT107I GGYRCC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 606
BssECI CCNNGG 2 cut(s) 424, 545
BssMI GATC 1 cut(s) 520
BssNI GRCGYC 1 cut(s) 106
BssSI CACGAG 1 cut(s) 360
BssT1I CCWWGG 2 cut(s) 424, 545
Bst2BI CACGAG 1 cut(s) 360
Bst6I CTCTTC 5 cut(s) 50, 126, 153, 332, 527
BstACI GRCGYC 1 cut(s) 106
BstC8I GCNNGC 3 cut(s) 313, 514, 561
BstDEI CTNAG 1 cut(s) 294
BstDSI CCRYGG 1 cut(s) 424
BstENI CCTNNNNNAGG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 633
BstFNI CGCG 1 cut(s) 352
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 2 cut(s) 108, 354
BstKTI GATC 1 cut(s) 523
BstMAI GTCTC 2 cut(s) 155, 363
BstMBI GATC 1 cut(s) 520
BstMWI GCNNNNNNNGC 4 cut(s) 349, 446, 509, 556
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 1 cut(s) 352
BstV1I GCAGC 4 cut(s) 320, 406, 412, 433
BstV2I GAAGAC 2 cut(s) 32, 305
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
BsuRI GGCC 3 cut(s) 6, 343, 461
BtgI CCRYGG 1 cut(s) 424
BtsCI GGATG 1 cut(s) 633
Cac8I GCNNGC 3 cut(s) 313, 514, 561
CfoI GCGC 2 cut(s) 108, 354
Cfr13I GGNCC 4 cut(s) 4, 5, 60, 603
Csp6I GTAC 1 cut(s) 475
CviAII CATG 3 cut(s) 27, 35, 425
CviQI GTAC 1 cut(s) 475
DdeI CTNAG 1 cut(s) 294
DinI GGCGCC 1 cut(s) 107
DpnI GATC 1 cut(s) 522
DpnII GATC 1 cut(s) 520
Eam1104I CTCTTC 5 cut(s) 50, 126, 153, 332, 527
EarI CTCTTC 5 cut(s) 50, 126, 153, 332, 527
EciI GGCGGA 3 cut(s) 41, 152, 291
Eco130I CCWWGG 2 cut(s) 424, 545
Eco147I AGGCCT 1 cut(s) 343
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 2 cut(s) 60, 603
Eco57I CTGAAG 3 cut(s) 234, 507, 539
Eco88I CYCGRG 1 cut(s) 470
EcoNI CCTNNNNNAGG 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 369
EcoT14I CCWWGG 2 cut(s) 424, 545
EcoT38I GRGCYC 1 cut(s) 8
EgeI GGCGCC 1 cut(s) 107
EheI GGCGCC 1 cut(s) 107
ErhI CCWWGG 2 cut(s) 424, 545
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 3 cut(s) 30, 38, 428
FaiI YATR 5 cut(s) 28, 36, 188, 396, 426
FaqI GGGAC 2 cut(s) 616, 628
FatI CATG 3 cut(s) 26, 34, 424
FauI CCCGC 1 cut(s) 259
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 5 cut(s) 109, 309, 401, 420, 447
FokI GGATG 1 cut(s) 620
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 5 cut(s) 109, 309, 401, 420, 447
FspBI CTAG 1 cut(s) 513
GlaI GCGC 2 cut(s) 107, 353
GluI GCNGC 5 cut(s) 109, 309, 401, 420, 447
GsuI CTGGAG 1 cut(s) 633
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 3 cut(s) 6, 343, 461
HapII CCGG 1 cut(s) 380
HhaI GCGC 2 cut(s) 108, 354
Hin1I GRCGYC 1 cut(s) 106
Hin1II CATG 3 cut(s) 30, 38, 428
Hin6I GCGC 2 cut(s) 106, 352
HinP1I GCGC 2 cut(s) 106, 352
HinfI GANTC 3 cut(s) 212, 391, 621
HpaII CCGG 1 cut(s) 380
HphI GGTGA 2 cut(s) 181, 541
Hpy166II GTNNAC 1 cut(s) 454
Hpy188I TCNGA 1 cut(s) 297
Hpy188III TCNNGA 3 cut(s) 231, 241, 494
Hpy8I GTNNAC 1 cut(s) 454
Hpy99I CGWCG 2 cut(s) 60, 212
HpyAV CCTTC 4 cut(s) 137, 231, 258, 658
HpyCH4V TGCA 1 cut(s) 34
HpyF10VI GCNNNNNNNGC 4 cut(s) 349, 446, 509, 556
HpyF3I CTNAG 1 cut(s) 294
Hsp92I GRCGYC 1 cut(s) 106
Hsp92II CATG 3 cut(s) 30, 38, 428
HspAI GCGC 2 cut(s) 106, 352
KasI GGCGCC 1 cut(s) 105
Kzo9I GATC 1 cut(s) 520
LmnI GCTCC 2 cut(s) 70, 580
Lsp1109I GCAGC 4 cut(s) 320, 406, 412, 433
LweI GCATC 1 cut(s) 559
MaeI CTAG 1 cut(s) 513
MaeIII GTNAC 1 cut(s) 76
MalI GATC 1 cut(s) 522
MboI GATC 1 cut(s) 520
MboII GAAGA 8 cut(s) 32, 37, 143, 170, 310, 349, 544, 545
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 3 cut(s) 83, 369, 537
Mly113I GGCGCC 1 cut(s) 106
MlyI GAGTC 2 cut(s) 206, 630
MseI TTAA 2 cut(s) 260, 597
MslI CAYNNNNRTG 2 cut(s) 25, 429
MspA1I CMGCKG 2 cut(s) 403, 559
MspI CCGG 1 cut(s) 380
MvnI CGCG 1 cut(s) 352
MwoI GCNNNNNNNGC 4 cut(s) 349, 446, 509, 556
NarI GGCGCC 1 cut(s) 106
NcoI CCATGG 1 cut(s) 424
NdeII GATC 1 cut(s) 520
NheI GCTAGC 1 cut(s) 512
NlaIII CATG 3 cut(s) 30, 38, 428
NlaIV GGNNCC 5 cut(s) 6, 62, 72, 107, 604
NmuCI GTSAC 1 cut(s) 76
PaeR7I CTCGAG 1 cut(s) 470
PceI AGGCCT 1 cut(s) 343
PfeI GAWTC 1 cut(s) 391
PkrI GCNGC 5 cut(s) 110, 310, 402, 421, 448
PleI GAGTC 2 cut(s) 206, 629
PluTI GGCGCC 1 cut(s) 109
PpsI GAGTC 2 cut(s) 206, 629
PshBI ATTAAT 1 cut(s) 260
PspN4I GGNNCC 5 cut(s) 6, 62, 72, 107, 604
PspOMI GGGCCC 1 cut(s) 4
PspPI GGNCC 4 cut(s) 4, 5, 60, 603
PspXI VCTCGAGB 1 cut(s) 470
PsuI RGATCY 1 cut(s) 520
PvuII CAGCTG 2 cut(s) 403, 559
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
RseI CAYNNNNRTG 2 cut(s) 25, 429
SaqAI TTAA 2 cut(s) 260, 597
SatI GCNGC 5 cut(s) 109, 309, 401, 420, 447
Sau3AI GATC 1 cut(s) 520
Sau96I GGNCC 4 cut(s) 4, 5, 60, 603
SchI GAGTC 2 cut(s) 206, 630
SduI GDGCHC 1 cut(s) 8
SetI ASST 7 cut(s) 405, 480, 531, 561, 585, 619, 662
SfaNI GCATC 1 cut(s) 559
SfoI GGCGCC 1 cut(s) 107
Sfr274I CTCGAG 1 cut(s) 470
SinI GGWCC 2 cut(s) 60, 603
SlaI CTCGAG 1 cut(s) 470
SmiMI CAYNNNNRTG 2 cut(s) 25, 429
SmlI CTYRAG 4 cut(s) 239, 470, 494, 524
SmoI CTYRAG 4 cut(s) 239, 470, 494, 524
Sse9I AATT 3 cut(s) 83, 369, 537
SseBI AGGCCT 1 cut(s) 343
SsiI CCGC 5 cut(s) 52, 109, 137, 266, 276
SspDI GGCGCC 1 cut(s) 105
SspMI CTAG 1 cut(s) 513
StuI AGGCCT 1 cut(s) 343
StyI CCWWGG 2 cut(s) 424, 545
TaqI TCGA 2 cut(s) 471, 662
TasI AATT 3 cut(s) 83, 369, 537
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 2 cut(s) 260, 597
Tru9I TTAA 2 cut(s) 260, 597
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 4 cut(s) 308, 400, 419, 446
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 1 cut(s) 383
TspGWI ACGGA 1 cut(s) 73
VpaK11BI GGWCC 2 cut(s) 60, 603
VspI ATTAAT 1 cut(s) 260
XagI CCTNNNNNAGG 1 cut(s) 252
XapI RAATTY 1 cut(s) 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 431
XhoI CTCGAG 1 cut(s) 470
XmiI GTMKAC 1 cut(s) 453
XspI CTAG 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.