Rh6CG395400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0015827
Physical Location & Seq
Forward (+)
58280640 .. 58284762
4123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG395400.1

Sequence Viewer

Length: 1647 bp
ATGATGTTATACTACACATCTCTACCCTCCTCTCTACTTCTGGCCACTTTTCTCTTTCTCTTCTTCTCGCACGGGGATTCTCACTTTTCAACCCTCCAACTCCAAACACCACCCCCCTCTACTTCACCAGGATGGGATTTTGAAGTGGGATTTTCAGGGAAGTCTCTTGTGGAGGGACCCTTTAGTGCTCCGATTGAGACCTCGTCGGTTGTGTTAGCTGCGCAGCGAACACGCCGGAAAGATCCTCTTGATGGGTTCAAGAGGTACACAGGAGGTTGGAATATCAGTGAGCGCCATTACTGGGCTTCTGTGGGTTTTACTGCAGTTCCCCTGTTTTCCATTGCTGCAATCTGGTTCCTGGGCTTTGGTTTGTGCTTGCTGCTCATCTGTATCTGTCACTTTTGCTGTCAAAGACAGGCTTATGGCTACTCTCGAACAGCCTATGTTCTTTCTCTTATATTCCTTGTACTATTCAGCATTACCGCAATGATTGGCTGTGTTGTGTTATACACCGGTCAAAAGAAGTTTCATGATAGCTCAACAAGTACATTGGAGTATGTCGTAAATCAGGCAGATATTACAGTTGGCCAATTAAGGAATGCATCAGATTATCTTGCTGCAGCTAAGCAGCTTGGAGTGGATCAAGTGTATTTACCTGCTAACATTCAAACTGATATAGATCAGATTGGTGGAAAGCTCAATTCCTCTGCTAACACCCTTGCTCGGCAAACAACAGAGAACTCAGATGACATTAGAGATCTCTTGGATTCTGTGAGACTGGCACTTATAATAATTGCTGCCATTATGCTTGTCTTGACATTTTTGGGATTCTTGTTTTCAATGTTCGGCATGCAATTGCTTGTGTACATACTAGTGATCACGGGATGGATACTTGTTGCTGGAACACTTATTCTGTGTGGCACATTCCTTCTTCTTCATAATGTGGCTGCTGATACCTGTGTTGCAATGGATGAATGGGTTCAAAACCCAACTGCTCATACAGCATTAGATGAAATACTGCCCTGTGTGGACAGTGCAACCGCCCAAGAAACCATGTTGCGAAGCAAGGAAGTCACTGCTCAACTAGTCAATTTGATCAATGAGGTCATCACCAATGTCTCAAACATAAATTTTGCTGCCAACTTCGTGCCAATGTACTTCAATCAATCTGGTCCATTGGTGCCACTCCTCTGCAACCCATTTTCTCCCGACTTGACTGATCGGGCTTGCACTGCTGGCGAAGTGAACTTAAACAATGCAACAGAGGTATGGGGAAGCTATGTCTGCCAGGTTACACCTAGTGGGATTTGCACCACAACAGGGCGTTTGACCCCGACATTCTACAGCCAGATGATTACTGGAGTAAGTCTGAGTAATGGCTTGTATAACTATGCTCCTGTACTCGTTCAACTACAAGACTGCACGTTTGTGAGGGAAACTTTTAGTGACATATACAGGGATCATTGTCCTGGCCTAAGAAGATACAGTAGATGGATCTACATTGGCTTGGTGATGGTTTCTACTTCAGTTATGCTCTCACTCGTGTTCTGGGTGATATATGGGAGAGAACGACGCCACCGTGTTTATACTAAAGAGTCAATGGCCGAATCAGCTCAAGGTACTGAAGGGGAAAAGGACATTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

548

Amino Acids

60.66

Weight (kDa)

5.41

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015827)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25270
fragaria_vesca FvH4_2g28370
malus_domestica MD15G1129700.v1.1
prunus_persica Prupe.1G485200_v2.0.a1
pyrus_communis pycom15g11690
rosa_chinensis RchiOBHm_Chr6g0297331
rosa_laevigata RLG00000011610
rosa_multiflora Rmu_sc0004535.1_g000010
rosa_roxburghii Rroxscaffold_7G00170640
rosa_rugosa Rorug06G0270800
rosa_samantha Rh6AG382200 Rh6BG390200 Rh6CG395400 Rh6DG382200
rosa_wichuraiana Rw6G033290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 788
Acc16I TGCGCA 1 cut(s) 222
Acc36I ACCTGC 1 cut(s) 664
AccB1I GGYRCC 1 cut(s) 1180
AciI CCGC 2 cut(s) 483, 1041
AclWI GGATC 4 cut(s) 236, 648, 1467, 1502
AcoI YGGCCR 3 cut(s) 42, 586, 1602
AcsI RAATTY 1 cut(s) 1129
AcuI CTGAAG 2 cut(s) 1509, 1644
AcyI GRCGYC 1 cut(s) 1573
AdeI CACNNNGTG 1 cut(s) 1301
AfaI GTAC 7 cut(s) 266, 468, 547, 866, 1157, 1401, 1621
AfiI CCNNNNNNNGG 4 cut(s) 251, 301, 723, 1320
AgeI ACCGGT 1 cut(s) 512
AgsI TTSAA 8 cut(s) 90, 143, 259, 668, 840, 983, 1162, 1409
AhlI ACTAGT 2 cut(s) 871, 1084
AjnI CCWGG 4 cut(s) 127, 357, 1287, 1468
AleI CACNNNNGTG 1 cut(s) 1427
AluBI AGCT 7 cut(s) 218, 537, 623, 631, 697, 1278, 1613
AluI AGCT 7 cut(s) 218, 537, 623, 631, 697, 1278, 1613
Alw21I GWGCWC 1 cut(s) 190
Alw26I GTCTC 4 cut(s) 168, 191, 769, 1123
AlwI GGATC 4 cut(s) 236, 648, 1467, 1502
AoxI GGCC 4 cut(s) 42, 586, 1471, 1602
ApoI RAATTY 1 cut(s) 1129
ArsI GACNNNNNNTTYG 2 cut(s) 1409, 1441
AsiGI ACCGGT 1 cut(s) 512
Asp700I GAANNNNTTC 1 cut(s) 978
AspLEI GCGC 2 cut(s) 223, 294
AspS9I GGNCC 2 cut(s) 176, 1172
AsuHPI GGTGA 4 cut(s) 117, 1102, 1522, 1564
AvaII GGWCC 2 cut(s) 176, 1172
BaeI ACNNNNGTAYC 2 cut(s) 945, 978
BalI TGGCCA 2 cut(s) 44, 588
BanI GGYRCC 1 cut(s) 1180
BauI CACGAG 1 cut(s) 1541
Bbv12I GWGCWC 1 cut(s) 190
BccI CCATC 5 cut(s) 126, 245, 879, 1485, 1507
BciT130I CCWGG 4 cut(s) 129, 359, 1289, 1470
BciVI GTATCC 1 cut(s) 882
BclI TGATCA 2 cut(s) 876, 1095
BcoDI GTCTC 4 cut(s) 168, 191, 769, 1123
BcuI ACTAGT 2 cut(s) 871, 1084
BfaI CTAG 3 cut(s) 872, 1085, 1299
BfmI CTRYAG 3 cut(s) 321, 618, 1342
BfoI RGCGCY 1 cut(s) 295
BfuAI ACCTGC 1 cut(s) 664
BfuI GTATCC 1 cut(s) 882
BglII AGATCT 1 cut(s) 757
BlpI GCTNAGC 1 cut(s) 624
Bme1390I CCNGG 4 cut(s) 129, 359, 1289, 1470
Bme18I GGWCC 2 cut(s) 176, 1172
BmgT120I GGNCC 2 cut(s) 176, 1172
BmiI GGNNCC 4 cut(s) 177, 178, 356, 1182
BmrFI CCNGG 4 cut(s) 129, 359, 1289, 1470
BmrI ACTGGG 1 cut(s) 310
BmsI GCATC 1 cut(s) 611
BmuI ACTGGG 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 1380
Bpu1102I GCTNAGC 1 cut(s) 624
BpuEI CTTGAG 1 cut(s) 1599
BsaBI GATNNNNATC 1 cut(s) 678
BsaHI GRCGYC 1 cut(s) 1573
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 358
BsaWI WCCGGW 1 cut(s) 512
Bsc4I CCNNNNNNNGG 4 cut(s) 251, 301, 723, 1320
Bse118I RCCGGY 1 cut(s) 512
Bse1I ACTGG 3 cut(s) 305, 783, 1363
Bse3DI GCAATG 3 cut(s) 339, 492, 972
Bse8I GATNNNNATC 1 cut(s) 678
BseBI CCWGG 4 cut(s) 129, 359, 1289, 1470
BseDI CCNNGG 1 cut(s) 358
BseGI GGATG 3 cut(s) 137, 890, 976
BseJI GATNNNNATC 1 cut(s) 678
BseLI CCNNNNNNNGG 4 cut(s) 251, 301, 723, 1320
BseMI GCAATG 3 cut(s) 339, 492, 972
BseMII CTCAG 2 cut(s) 756, 1361
BseNI ACTGG 3 cut(s) 305, 783, 1363
BseRI GAGGAG 2 cut(s) 19, 1178
BsgI GTGCAG 1 cut(s) 1405
BshFI GGCC 4 cut(s) 44, 588, 1473, 1604
BshNI GGYRCC 1 cut(s) 1180
BshTI ACCGGT 1 cut(s) 512
BsiHKAI GWGCWC 1 cut(s) 190
BsiSI CCGG 2 cut(s) 235, 513
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 4 cut(s) 251, 301, 723, 1320
BsmAI GTCTC 4 cut(s) 168, 191, 769, 1123
BsmFI GGGAC 1 cut(s) 189
BsmI GAATGC 1 cut(s) 604
BsnI GGCC 4 cut(s) 44, 588, 1473, 1604
Bso31I GGTCTC 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 190
Bsp1407I TGTACA 1 cut(s) 864
Bsp143I GATC 9 cut(s) 241, 640, 679, 757, 876, 1095, 1219, 1459, 1494
Bsp1720I GCTNAGC 1 cut(s) 624
BspACI CCGC 2 cut(s) 483, 1041
BspANI GGCC 4 cut(s) 44, 588, 1473, 1604
BspCNI CTCAG 2 cut(s) 755, 1362
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 4 cut(s) 177, 178, 356, 1182
BspMAI CTGCAG 2 cut(s) 325, 622
BspMI ACCTGC 1 cut(s) 664
BspPI GGATC 4 cut(s) 236, 648, 1467, 1502
BspT107I GGYRCC 1 cut(s) 1180
BspTNI GGTCTC 1 cut(s) 191
BsrDI GCAATG 3 cut(s) 339, 492, 972
BsrFI RCCGGY 1 cut(s) 512
BsrGI TGTACA 1 cut(s) 864
BsrI ACTGG 3 cut(s) 305, 783, 1363
BssAI RCCGGY 1 cut(s) 512
BssECI CCNNGG 1 cut(s) 358
BssMI GATC 9 cut(s) 241, 640, 679, 757, 876, 1095, 1219, 1459, 1494
BssNI GRCGYC 1 cut(s) 1573
BssSI CACGAG 1 cut(s) 1541
Bst2BI CACGAG 1 cut(s) 1541
Bst2UI CCWGG 4 cut(s) 129, 359, 1289, 1470
Bst4CI ACNGT 4 cut(s) 583, 1034, 1487, 1580
Bst6I CTCTTC 1 cut(s) 65
BstACI GRCGYC 1 cut(s) 1573
BstAUI TGTACA 1 cut(s) 864
BstC8I GCNNGC 4 cut(s) 377, 851, 1228, 1237
BstDEI CTNAG 4 cut(s) 624, 742, 1370, 1475
BstF5I GGATG 3 cut(s) 137, 890, 976
BstH2I RGCGCY 1 cut(s) 295
BstHHI GCGC 2 cut(s) 223, 294
BstKTI GATC 9 cut(s) 244, 643, 682, 760, 879, 1098, 1222, 1462, 1497
BstMAI GTCTC 4 cut(s) 168, 191, 769, 1123
BstMBI GATC 9 cut(s) 241, 640, 679, 757, 876, 1095, 1219, 1459, 1494
BstMWI GCNNNNNNNGC 5 cut(s) 1001, 1232, 1236, 1284, 1610
BstNI CCWGG 4 cut(s) 129, 359, 1289, 1470
BstNSI RCATGY 1 cut(s) 853
BstSCI CCNGG 4 cut(s) 127, 357, 1287, 1468
BstSFI CTRYAG 3 cut(s) 321, 618, 1342
BstX2I RGATCY 3 cut(s) 241, 757, 1494
BstYI RGATCY 3 cut(s) 241, 757, 1494
BsuI GTATCC 1 cut(s) 882
BsuRI GGCC 4 cut(s) 44, 588, 1473, 1604
BtsCI GGATG 3 cut(s) 137, 890, 976
BtsI GCAGTG 2 cut(s) 1074, 1230
BtsIMutI CAGTG 4 cut(s) 292, 1039, 1074, 1230
BveI ACCTGC 1 cut(s) 664
Cac8I GCNNGC 4 cut(s) 377, 851, 1228, 1237
CciI TCATGA 1 cut(s) 529
CfoI GCGC 2 cut(s) 223, 294
Cfr10I RCCGGY 1 cut(s) 512
Cfr13I GGNCC 2 cut(s) 176, 1172
CseI GACGC 1 cut(s) 1581
Csp6I GTAC 7 cut(s) 265, 467, 546, 865, 1156, 1400, 1620
CspAI ACCGGT 1 cut(s) 512
CviAII CATG 3 cut(s) 530, 850, 1054
CviQI GTAC 7 cut(s) 265, 467, 546, 865, 1156, 1400, 1620
DdeI CTNAG 4 cut(s) 624, 742, 1370, 1475
DpnI GATC 9 cut(s) 243, 642, 681, 759, 878, 1097, 1221, 1461, 1496
DpnII GATC 9 cut(s) 241, 640, 679, 757, 876, 1095, 1219, 1459, 1494
DraIII CACNNNGTG 1 cut(s) 1301
EaeI YGGCCR 3 cut(s) 42, 586, 1602
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Eco31I GGTCTC 1 cut(s) 191
Eco47I GGWCC 2 cut(s) 176, 1172
Eco57I CTGAAG 2 cut(s) 1509, 1644
EcoO109I RGGNCCY 1 cut(s) 176
EcoRII CCWGG 4 cut(s) 127, 357, 1287, 1468
EcoT22I ATGCAT 1 cut(s) 604
FaeI CATG 3 cut(s) 533, 853, 1057
FalI AAGNNNNNCTT 4 cut(s) 231, 263, 403, 435
FaqI GGGAC 1 cut(s) 189
FatI CATG 3 cut(s) 529, 849, 1053
FbaI TGATCA 2 cut(s) 876, 1095
FokI GGATG 3 cut(s) 144, 897, 983
FspBI CTAG 3 cut(s) 872, 1085, 1299
FspI TGCGCA 1 cut(s) 222
GlaI GCGC 2 cut(s) 222, 293
GsuI CTGGAG 1 cut(s) 1380
HaeII RGCGCY 1 cut(s) 295
HaeIII GGCC 4 cut(s) 44, 588, 1473, 1604
HapII CCGG 2 cut(s) 235, 513
HgaI GACGC 1 cut(s) 1581
HhaI GCGC 2 cut(s) 223, 294
Hin1I GRCGYC 1 cut(s) 1573
Hin1II CATG 3 cut(s) 533, 853, 1057
Hin6I GCGC 2 cut(s) 221, 292
HinP1I GCGC 2 cut(s) 221, 292
HinfI GANTC 5 cut(s) 77, 767, 828, 1595, 1607
HpaII CCGG 2 cut(s) 235, 513
HphI GGTGA 4 cut(s) 117, 1102, 1522, 1564
Hpy166II GTNNAC 4 cut(s) 267, 865, 1030, 1246
Hpy188I TCNGA 5 cut(s) 192, 607, 684, 745, 1371
Hpy188III TCNNGA 7 cut(s) 248, 259, 432, 530, 814, 1208, 1644
Hpy8I GTNNAC 4 cut(s) 267, 865, 1030, 1246
Hpy99I CGWCG 2 cut(s) 208, 1575
HpyAV CCTTC 2 cut(s) 938, 1619
HpyCH4III ACNGT 4 cut(s) 583, 1034, 1487, 1580
HpyCH4IV ACGT 1 cut(s) 1424
HpyF10VI GCNNNNNNNGC 5 cut(s) 1001, 1232, 1236, 1284, 1610
HpyF3I CTNAG 4 cut(s) 624, 742, 1370, 1475
HpySE526I ACGT 1 cut(s) 1424
Hsp92I GRCGYC 1 cut(s) 1573
Hsp92II CATG 3 cut(s) 533, 853, 1057
HspAI GCGC 2 cut(s) 221, 292
KflI GGGWCCC 1 cut(s) 176
Ksp22I TGATCA 2 cut(s) 876, 1095
Kzo9I GATC 9 cut(s) 241, 640, 679, 757, 876, 1095, 1219, 1459, 1494
LmnI GCTCC 2 cut(s) 193, 1399
LweI GCATC 1 cut(s) 611
MaeI CTAG 3 cut(s) 872, 1085, 1299
MaeII ACGT 1 cut(s) 1424
MaeIII GTNAC 4 cut(s) 395, 1072, 1291, 1445
MalI GATC 9 cut(s) 243, 642, 681, 759, 878, 1097, 1221, 1461, 1496
MboI GATC 9 cut(s) 241, 640, 679, 757, 876, 1095, 1219, 1459, 1494
MboII GAAGA 5 cut(s) 52, 55, 923, 926, 1491
MfeI CAATTG 1 cut(s) 854
MflI RGATCY 3 cut(s) 241, 757, 1494
MhlI GDGCHC 1 cut(s) 190
MlsI TGGCCA 2 cut(s) 44, 588
MluCI AATT 6 cut(s) 590, 700, 792, 854, 1090, 1129
MluNI TGGCCA 2 cut(s) 44, 588
MlyI GAGTC 1 cut(s) 1604
MmeI TCCRAC 2 cut(s) 121, 257
Mox20I TGGCCA 2 cut(s) 44, 588
Mph1103I ATGCAT 1 cut(s) 604
MroXI GAANNNNTTC 1 cut(s) 978
MscI TGGCCA 2 cut(s) 44, 588
MseI TTAA 2 cut(s) 593, 1250
MslI CAYNNNNRTG 3 cut(s) 130, 872, 1427
Msp20I TGGCCA 2 cut(s) 44, 588
MspI CCGG 2 cut(s) 235, 513
MspR9I CCNGG 4 cut(s) 129, 359, 1289, 1470
MunI CAATTG 1 cut(s) 854
Mva1269I GAATGC 1 cut(s) 604
MvaI CCWGG 4 cut(s) 129, 359, 1289, 1470
MwoI GCNNNNNNNGC 5 cut(s) 1001, 1232, 1236, 1284, 1610
NdeII GATC 9 cut(s) 241, 640, 679, 757, 876, 1095, 1219, 1459, 1494
NlaIII CATG 3 cut(s) 533, 853, 1057
NlaIV GGNNCC 4 cut(s) 177, 178, 356, 1182
NmeAIII GCCGAG 1 cut(s) 703
NmuCI GTSAC 3 cut(s) 395, 1072, 1445
NsbI TGCGCA 1 cut(s) 222
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 1 cut(s) 853
OliI CACNNNNGTG 1 cut(s) 1427
PaeI GCATGC 1 cut(s) 853
PagI TCATGA 1 cut(s) 529
PcsI WCGNNNNNNNCGW 1 cut(s) 1576
PctI GAATGC 1 cut(s) 604
PdmI GAANNNNTTC 1 cut(s) 978
PfeI GAWTC 4 cut(s) 77, 767, 828, 1607
PflFI GACNNNGTC 1 cut(s) 202
PinAI ACCGGT 1 cut(s) 512
PleI GAGTC 1 cut(s) 1603
PpsI GAGTC 1 cut(s) 1603
PpuMI RGGWCCY 1 cut(s) 176
PsiI TTATAA 1 cut(s) 788
Psp5II RGGWCCY 1 cut(s) 176
Psp6I CCWGG 4 cut(s) 127, 357, 1287, 1468
PspGI CCWGG 4 cut(s) 127, 357, 1287, 1468
PspN4I GGNNCC 4 cut(s) 177, 178, 356, 1182
PspPI GGNCC 2 cut(s) 176, 1172
PspPPI RGGWCCY 1 cut(s) 176
PstI CTGCAG 2 cut(s) 325, 622
PsuI RGATCY 3 cut(s) 241, 757, 1494
PsyI GACNNNGTC 1 cut(s) 202
RsaI GTAC 7 cut(s) 266, 468, 547, 866, 1157, 1401, 1621
RsaNI GTAC 7 cut(s) 265, 467, 546, 865, 1156, 1400, 1620
RseI CAYNNNNRTG 3 cut(s) 130, 872, 1427
SaqAI TTAA 2 cut(s) 593, 1250
Sau3AI GATC 9 cut(s) 241, 640, 679, 757, 876, 1095, 1219, 1459, 1494
Sau96I GGNCC 2 cut(s) 176, 1172
SchI GAGTC 1 cut(s) 1604
ScrFI CCNGG 4 cut(s) 129, 359, 1289, 1470
SduI GDGCHC 1 cut(s) 190
SfaNI GCATC 1 cut(s) 611
SfcI CTRYAG 3 cut(s) 321, 618, 1342
SinI GGWCC 2 cut(s) 176, 1172
SmiMI CAYNNNNRTG 3 cut(s) 130, 872, 1427
SmlI CTYRAG 1 cut(s) 1614
SmoI CTYRAG 1 cut(s) 1614
SpeI ACTAGT 2 cut(s) 871, 1084
SphI GCATGC 1 cut(s) 853
Sse9I AATT 6 cut(s) 590, 700, 792, 854, 1090, 1129
SsiI CCGC 2 cut(s) 483, 1041
SspMI CTAG 3 cut(s) 872, 1085, 1299
StyD4I CCNGG 4 cut(s) 127, 357, 1287, 1468
TaaI ACNGT 4 cut(s) 583, 1034, 1487, 1580
TaiI ACGT 1 cut(s) 1427
TaqI TCGA 1 cut(s) 433
TasI AATT 6 cut(s) 590, 700, 792, 854, 1090, 1129
TatI WGTACW 5 cut(s) 466, 545, 864, 1155, 1399
TfiI GAWTC 4 cut(s) 77, 767, 828, 1607
Tru1I TTAA 2 cut(s) 593, 1250
Tru9I TTAA 2 cut(s) 593, 1250
TscAI CASTG 4 cut(s) 292, 1039, 1081, 1237
TseFI GTSAC 3 cut(s) 395, 1072, 1445
Tsp45I GTSAC 3 cut(s) 395, 1072, 1445
TspDTI ATGAA 4 cut(s) 518, 926, 987, 1026
TspRI CASTG 4 cut(s) 292, 1039, 1081, 1237
Tth111I GACNNNGTC 1 cut(s) 202
VpaK11BI GGWCC 2 cut(s) 176, 1172
XapI RAATTY 1 cut(s) 1129
XceI RCATGY 1 cut(s) 853
XcmI CCANNNNNNNNNTGG 1 cut(s) 1355
XmnI GAANNNNTTC 1 cut(s) 978
XspI CTAG 3 cut(s) 872, 1085, 1299
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.