Rh6CG436700

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0011466
Physical Location & Seq
Reverse (-)
61610552 .. 61614083
3532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG436700.1

Sequence Viewer

Length: 1134 bp
ATGGCCGAACCAGGTGGCTCTGACAAGAGGAGTTGGTGCTCGGTGCCGGAGAAGTTTCAGCTTCATGGAGCCATGTTGGCTTTGCAGTTCGGCTATGCCGGTTTCCATGTCGTCTCTAGATTTGCTCTCAATATGGGCATTTCTAAGCTTGTCTTTCCCGTTTACCGGAACATCATCGCTTTGCTTCTACTCCTGCCCTTTGCCTACTTTCTGGAAAAGAAGGACAGGCCTCCGATCAATCTCAACTTTCTCGTTCAGTTCTTTTGCCTTGCGCTTATCGGAATAACAGCAAACCAAGGATTCTACTTGCTGGGATTAGACAACACATCACCCACCTTTGCTTCCGCTATACAAAACTCCGTCCCAGCCATTACCTTTCTCATGGCAGTCATACTCAGAATCGAGCAAGTAAGACTAAACCGCAAGGACGGTATTGCAAAGGTAGCCGGAACTATTTTCTGTGTGGCTGGAGCATCAGTGATTACTCTATACAAAGGTCCAGCCATATACAGCCCAACACCACCACTGCAGACGATGATGATGAGCAGTGCCTCATCATCAGTATCAATGTCGAATTTCACTACTAGCGCCATGTCAGTACTTGGTGATGCAAAGGGTAAGAGCTGGACTCTAGGTTGCCTCTACCTGATCGGCCATTGCCTCTCGTGGTCCGCCTGGCTCGTCCTGCAAAGCCCGGTTCTCAAGAAGTACCCGGCCCGGCTCTCTGTCACCTCCTACACTTGTTTCTTTGGTCTAATTCAGTTCATCATTATCGCTGCGGTTTTCGAGAGAGACGCTCAGGCTTGGATCTTCCACAATGGTGGAGAAGCCTTCAGCATCCTCTATGCGGTCAGTACTCAGTACTATAGAATTATATCTGGAAAAGTCTATTTGGATTTAAAGCTTAGTGTAATTAATGCTATAAGATTAATTTTTTCTACTAAAATTGCTTTAATTTTTAACGTCTTCTTATCTATGACTTCAAGGAAGATTTTTCAGCCAACAGCGACCTCCCTGAATGACAAGCACCCTCAACCTAATTGTTTCCTTTGTCTTTCCTTTTCACAAAAACGAATTGGGAGTGAAGCCTTCTCAGTTTTCACTCTCTCGACGCCCTTTGTGTCAATTTCATAG

Protein Analysis

377

Amino Acids

41.58

Weight (kDa)

9.58

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 25 - 162 1.1e-13 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011466)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75500 AT1G75500
fragaria_vesca FvH4_2g38920 FvH4_2g38920
malus_domestica MD08G1022400.v1.1 MD15G1019700.v1.1
prunus_persica Prupe.1G371800_v2.0.a1 Prupe.1G371800_v2.0.a1
pyrus_communis pycom08g01730 pycom15g01640
rosa_chinensis RchiOBHm_Chr6g0302011
rosa_laevigata RLG00000011203
rosa_multiflora Rmu_sc0006892.1_g000006
rosa_roxburghii Rroxscaffold_7G00166190
rosa_rugosa Rorug06G0311000
rosa_samantha Rh6AG423700 Rh6BG430300 Rh6CG436700 Rh6DG423700
rosa_wichuraiana Rw6G036700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 43
AciI CCGC 5 cut(s) 345, 421, 672, 779, 848
AclWI GGATC 1 cut(s) 815
AcoI YGGCCR 2 cut(s) 3, 652
AcsI RAATTY 1 cut(s) 574
AcuI CTGAAG 1 cut(s) 817
AcyI GRCGYC 1 cut(s) 1112
AfaI GTAC 4 cut(s) 600, 710, 856, 863
AfiI CCNNNNNNNGG 2 cut(s) 165, 847
AgsI TTSAA 1 cut(s) 984
AjnI CCWGG 2 cut(s) 10, 674
AleI CACNNNNGTG 1 cut(s) 819
AluBI AGCT 4 cut(s) 61, 148, 624, 904
AluI AGCT 4 cut(s) 61, 148, 624, 904
Alw21I GWGCWC 1 cut(s) 41
Alw26I GTCTC 2 cut(s) 118, 786
AlwI GGATC 1 cut(s) 815
AoxI GGCC 4 cut(s) 3, 227, 652, 714
ApeKI GCWGC 1 cut(s) 776
ApoI RAATTY 1 cut(s) 574
AseI ATTAAT 2 cut(s) 915, 929
AspLEI GCGC 2 cut(s) 274, 590
AspS9I GGNCC 3 cut(s) 497, 669, 715
AsuC2I CCSGG 3 cut(s) 695, 713, 718
AsuHPI GGTGA 3 cut(s) 321, 617, 721
AvaII GGWCC 2 cut(s) 497, 669
BanI GGYRCC 1 cut(s) 43
BauI CACGAG 1 cut(s) 664
BbsI GAAGAC 1 cut(s) 958
Bbv12I GWGCWC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 763
BciT130I CCWGG 2 cut(s) 12, 676
BcnI CCSGG 3 cut(s) 695, 713, 718
BcoDI GTCTC 2 cut(s) 118, 786
BfaI CTAG 3 cut(s) 117, 585, 632
BfmI CTRYAG 2 cut(s) 527, 865
BfoI RGCGCY 1 cut(s) 591
BglI GCCNNNNNGGC 1 cut(s) 77
BisI GCNGC 1 cut(s) 777
BlsI GCNGC 1 cut(s) 778
BmcAI AGTACT 3 cut(s) 600, 856, 863
Bme1390I CCNGG 5 cut(s) 12, 676, 695, 713, 718
Bme18I GGWCC 2 cut(s) 497, 669
BmgT120I GGNCC 3 cut(s) 497, 669, 715
BmiI GGNNCC 2 cut(s) 45, 70
BmrFI CCNGG 5 cut(s) 12, 676, 695, 713, 718
BmsI GCATC 3 cut(s) 482, 598, 846
BpiI GAAGAC 1 cut(s) 958
BplI GAGNNNNNCTC 4 cut(s) 613, 645, 781, 813
BpmI CTGGAG 1 cut(s) 489
Bpu10I CCTNAGC 1 cut(s) 798
BpuEI CTTGAG 1 cut(s) 686
BpuMI CCSGG 3 cut(s) 695, 713, 718
BsaHI GRCGYC 1 cut(s) 1112
BsaJI CCNNGG 1 cut(s) 295
BsaWI WCCGGW 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 847
Bse118I RCCGGY 1 cut(s) 98
Bse3DI GCAATG 1 cut(s) 655
BseBI CCWGG 2 cut(s) 12, 676
BseDI CCNNGG 1 cut(s) 295
BseGI GGATG 1 cut(s) 837
BseLI CCNNNNNNNGG 2 cut(s) 165, 847
BseMI GCAATG 1 cut(s) 655
BseMII CTCAG 4 cut(s) 409, 812, 872, 1107
BseRI GAGGAG 1 cut(s) 43
BseXI GCAGC 1 cut(s) 763
BseYI CCCAGC 2 cut(s) 310, 364
BshFI GGCC 4 cut(s) 5, 229, 654, 716
BshNI GGYRCC 1 cut(s) 43
BsiHKAI GWGCWC 1 cut(s) 41
BsiSI CCGG 7 cut(s) 47, 99, 166, 447, 695, 713, 718
BslFI GGGAC 1 cut(s) 347
BslI CCNNNNNNNGG 2 cut(s) 165, 847
BsmAI GTCTC 2 cut(s) 118, 786
BsmBI CGTCTC 2 cut(s) 118, 786
BsmFI GGGAC 1 cut(s) 347
BsnI GGCC 4 cut(s) 5, 229, 654, 716
Bsp1286I GDGCHC 1 cut(s) 41
Bsp143I GATC 3 cut(s) 234, 648, 807
BspACI CCGC 5 cut(s) 345, 421, 672, 779, 848
BspANI GGCC 4 cut(s) 5, 229, 654, 716
BspCNI CTCAG 4 cut(s) 408, 811, 871, 1106
BspLI GGNNCC 2 cut(s) 45, 70
BspMAI CTGCAG 1 cut(s) 531
BspPI GGATC 1 cut(s) 815
BspT107I GGYRCC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 655
BsrFI RCCGGY 1 cut(s) 98
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 1 cut(s) 295
BssMI GATC 3 cut(s) 234, 648, 807
BssNI GRCGYC 1 cut(s) 1112
BssSI CACGAG 1 cut(s) 664
BssT1I CCWWGG 1 cut(s) 295
Bst2BI CACGAG 1 cut(s) 664
Bst2UI CCWGG 2 cut(s) 12, 676
Bst4CI ACNGT 1 cut(s) 431
BstACI GRCGYC 1 cut(s) 1112
BstDEI CTNAG 6 cut(s) 144, 395, 798, 858, 905, 1093
BstF5I GGATG 1 cut(s) 837
BstH2I RGCGCY 1 cut(s) 591
BstHHI GCGC 2 cut(s) 274, 590
BstKTI GATC 3 cut(s) 237, 651, 810
BstMAI GTCTC 2 cut(s) 118, 786
BstMBI GATC 3 cut(s) 234, 648, 807
BstMWI GCNNNNNNNGC 3 cut(s) 77, 443, 685
BstNI CCWGG 2 cut(s) 12, 676
BstSCI CCNGG 5 cut(s) 10, 674, 693, 711, 716
BstSFI CTRYAG 2 cut(s) 527, 865
BstV1I GCAGC 1 cut(s) 763
BstV2I GAAGAC 1 cut(s) 958
BstX2I RGATCY 1 cut(s) 807
BstXI CCANNNNNNTGG 1 cut(s) 821
BstYI RGATCY 1 cut(s) 807
BsuRI GGCC 4 cut(s) 5, 229, 654, 716
BtgZI GCGATG 1 cut(s) 160
BtsCI GGATG 1 cut(s) 837
BtsI GCAGTG 2 cut(s) 524, 553
BtsIMutI CAGTG 3 cut(s) 483, 524, 553
CfoI GCGC 2 cut(s) 274, 590
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 3 cut(s) 497, 669, 715
CseI GACGC 2 cut(s) 803, 1120
CsiI ACCWGGT 1 cut(s) 10
Csp6I GTAC 4 cut(s) 599, 709, 855, 862
CviAII CATG 5 cut(s) 65, 73, 107, 382, 592
CviQI GTAC 4 cut(s) 599, 709, 855, 862
DdeI CTNAG 6 cut(s) 144, 395, 798, 858, 905, 1093
DpnI GATC 3 cut(s) 236, 650, 809
DpnII GATC 3 cut(s) 234, 648, 807
DraI TTTAAA 1 cut(s) 900
EaeI YGGCCR 2 cut(s) 3, 652
EciI GGCGGA 1 cut(s) 661
Eco130I CCWWGG 1 cut(s) 295
Eco147I AGGCCT 1 cut(s) 229
Eco47I GGWCC 2 cut(s) 497, 669
Eco57I CTGAAG 1 cut(s) 817
EcoRII CCWGG 2 cut(s) 10, 674
EcoT14I CCWWGG 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 295
Esp3I CGTCTC 2 cut(s) 118, 786
FaeI CATG 5 cut(s) 68, 76, 110, 385, 595
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 1 cut(s) 347
FatI CATG 5 cut(s) 64, 72, 106, 381, 591
Fnu4HI GCNGC 1 cut(s) 777
FokI GGATG 1 cut(s) 824
Fsp4HI GCNGC 1 cut(s) 777
FspBI CTAG 3 cut(s) 117, 585, 632
GlaI GCGC 2 cut(s) 273, 589
GluI GCNGC 1 cut(s) 777
GsaI CCCAGC 2 cut(s) 314, 368
GsuI CTGGAG 1 cut(s) 489
HaeII RGCGCY 1 cut(s) 591
HaeIII GGCC 4 cut(s) 5, 229, 654, 716
HapII CCGG 7 cut(s) 47, 99, 166, 447, 695, 713, 718
HgaI GACGC 2 cut(s) 803, 1120
HhaI GCGC 2 cut(s) 274, 590
Hin1I GRCGYC 1 cut(s) 1112
Hin1II CATG 5 cut(s) 68, 76, 110, 385, 595
Hin6I GCGC 2 cut(s) 272, 588
HinP1I GCGC 2 cut(s) 272, 588
HindIII AAGCTT 2 cut(s) 146, 902
HinfI GANTC 3 cut(s) 300, 399, 628
HpaII CCGG 7 cut(s) 47, 99, 166, 447, 695, 713, 718
HphI GGTGA 3 cut(s) 321, 617, 721
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 4 cut(s) 22, 234, 281, 398
Hpy188III TCNNGA 6 cut(s) 117, 212, 703, 787, 879, 1108
Hpy8I GTNNAC 1 cut(s) 163
Hpy99I CGWCG 1 cut(s) 1114
HpyAV CCTTC 3 cut(s) 214, 841, 1099
HpyCH4III ACNGT 1 cut(s) 431
HpyCH4IV ACGT 1 cut(s) 963
HpyCH4V TGCA 5 cut(s) 85, 437, 529, 611, 688
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 443, 685
HpyF3I CTNAG 6 cut(s) 144, 395, 798, 858, 905, 1093
HpySE526I ACGT 1 cut(s) 963
Hsp92I GRCGYC 1 cut(s) 1112
Hsp92II CATG 5 cut(s) 68, 76, 110, 385, 595
HspAI GCGC 2 cut(s) 272, 588
Kzo9I GATC 3 cut(s) 234, 648, 807
LmnI GCTCC 2 cut(s) 68, 470
Lsp1109I GCAGC 1 cut(s) 763
LweI GCATC 3 cut(s) 482, 598, 846
MabI ACCWGGT 1 cut(s) 10
MaeI CTAG 3 cut(s) 117, 585, 632
MaeII ACGT 1 cut(s) 963
MaeIII GTNAC 1 cut(s) 727
MalI GATC 3 cut(s) 236, 650, 809
MboI GATC 3 cut(s) 234, 648, 807
MboII GAAGA 3 cut(s) 802, 958, 1000
MflI RGATCY 1 cut(s) 807
MhlI GDGCHC 1 cut(s) 41
MlyI GAGTC 1 cut(s) 622
MnlI CCTC 9 cut(s) 21, 240, 562, 650, 671, 742, 851, 1021, 1041
MseI TTAA 5 cut(s) 899, 915, 929, 953, 960
MslI CAYNNNNRTG 1 cut(s) 819
MspI CCGG 7 cut(s) 47, 99, 166, 447, 695, 713, 718
MspR9I CCNGG 5 cut(s) 12, 676, 695, 713, 718
MvaI CCWGG 2 cut(s) 12, 676
MwoI GCNNNNNNNGC 3 cut(s) 77, 443, 685
NciI CCSGG 3 cut(s) 695, 713, 718
NdeII GATC 3 cut(s) 234, 648, 807
NlaIII CATG 5 cut(s) 68, 76, 110, 385, 595
NlaIV GGNNCC 2 cut(s) 45, 70
NmuCI GTSAC 1 cut(s) 727
OliI CACNNNNGTG 1 cut(s) 819
PceI AGGCCT 1 cut(s) 229
PfeI GAWTC 2 cut(s) 300, 399
PkrI GCNGC 1 cut(s) 778
PleI GAGTC 1 cut(s) 622
PpsI GAGTC 1 cut(s) 622
PshBI ATTAAT 2 cut(s) 915, 929
Psp6I CCWGG 2 cut(s) 10, 674
PspFI CCCAGC 2 cut(s) 310, 364
PspGI CCWGG 2 cut(s) 10, 674
PspN4I GGNNCC 2 cut(s) 45, 70
PspPI GGNCC 3 cut(s) 497, 669, 715
PstI CTGCAG 1 cut(s) 531
PsuI RGATCY 1 cut(s) 807
RsaI GTAC 4 cut(s) 600, 710, 856, 863
RsaNI GTAC 4 cut(s) 599, 709, 855, 862
RseI CAYNNNNRTG 1 cut(s) 819
SaqAI TTAA 5 cut(s) 899, 915, 929, 953, 960
SatI GCNGC 1 cut(s) 777
Sau3AI GATC 3 cut(s) 234, 648, 807
Sau96I GGNCC 3 cut(s) 497, 669, 715
ScaI AGTACT 3 cut(s) 600, 856, 863
SchI GAGTC 1 cut(s) 622
ScrFI CCNGG 5 cut(s) 12, 676, 695, 713, 718
SduI GDGCHC 1 cut(s) 41
SexAI ACCWGGT 1 cut(s) 10
SfaNI GCATC 3 cut(s) 482, 598, 846
SfcI CTRYAG 2 cut(s) 527, 865
SinI GGWCC 2 cut(s) 497, 669
SmiMI CAYNNNNRTG 1 cut(s) 819
SmlI CTYRAG 1 cut(s) 701
SmoI CTYRAG 1 cut(s) 701
SseBI AGGCCT 1 cut(s) 229
SsiI CCGC 5 cut(s) 345, 421, 672, 779, 848
SspMI CTAG 3 cut(s) 117, 585, 632
StuI AGGCCT 1 cut(s) 229
StyD4I CCNGG 5 cut(s) 10, 674, 693, 711, 716
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 1 cut(s) 431
TaiI ACGT 1 cut(s) 966
TaqI TCGA 4 cut(s) 402, 572, 786, 1109
TatI WGTACW 3 cut(s) 598, 854, 861
TfiI GAWTC 2 cut(s) 300, 399
Tru1I TTAA 5 cut(s) 899, 915, 929, 953, 960
Tru9I TTAA 5 cut(s) 899, 915, 929, 953, 960
TscAI CASTG 3 cut(s) 483, 531, 553
TseFI GTSAC 1 cut(s) 727
TseI GCWGC 1 cut(s) 776
Tsp45I GTSAC 1 cut(s) 727
TspDTI ATGAA 3 cut(s) 53, 754, 1119
TspGWI ACGGA 1 cut(s) 349
TspRI CASTG 3 cut(s) 483, 531, 553
VpaK11BI GGWCC 2 cut(s) 497, 669
VspI ATTAAT 2 cut(s) 915, 929
XapI RAATTY 1 cut(s) 574
XbaI TCTAGA 1 cut(s) 116
XspI CTAG 3 cut(s) 117, 585, 632
ZrmI AGTACT 3 cut(s) 600, 856, 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.