Rh6DG225700

Zeaxanthin epoxidase, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0018908
Physical Location & Seq
Reverse (-)
40418541 .. 40423196
4656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG225700.1

Sequence Viewer

Length: 777 bp
ATGACCTTCGACGACCAGAAGCCTGGAACCTCCGAGAAAGAGCTAGAGAAGAACCCTGCTCAGTTGAAAAAATATATCTTACGCAAGCTCGGAAAGGTACAAGATGAAGTAAGGGCTGTTATAGAAAACACTGAATTGGATGCTTTTATATCCACTCCAGTGAGATATAGGCACCCTTGGGAACTGCTTTGGGGAAATATTAGCAAAGGTGGTGTATGTGTTGCTGGAGACGCACTCCACCCCATGACCCCAGACATCGGCCAAGGTGGCTGTGCTGCATTAGAAGACGGTGTTGTATTAGCAAGATGTCTCGGTGAGGCTTTGTTGAAGAACCGGAGGCAAGAAATTAGAGATAAAGATGAAGAACGAAAAGAGGAATGTAAGAGGATTGAAATGGGGTTGAGGAATTATGCCAGCTGGGGACTCGAAGTAGTGTTGGAATCATGGGATAGTTTGAGGACTACAGGGTTTGTTTTGAATACATGGACAAATGCCTGGAAAGCCTTAGAAGCCATTGGTGTTGCTGATGCTCTTCGGCTAATAAATTACAGATTTCGTGGAGTAGACCATGAAGTTCGTTGTGTGCAAAGGAAGCTGTTGTTGGATTCCCTTGCAAATGAACTTTCTAGTGCCACAACCAGGTTCTCATCAAAAGTTGTTTCAATCGAAGAATCAGGCTACTTTAAGCTTCTACATCTTGCTGATGGAACCATCCTTAAAGTTAAGAGAGTCCAACACAAGACCAATAGGGATGTGCGTGTCCGGAAAGATCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_3

Protein Analysis

258

Amino Acids

29.33

Weight (kDa)

8.73

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 65 - 124 1.3e-06 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018908)

Species Orthologous Gene IDs
prunus_persica Prupe.8G167400_v2.0.a1 Prupe.8G167400_v2.0.a1 Prupe.I003800_v2.0.a1 Prupe.I004000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0278571
rosa_multiflora Rmu_sc0000258.1_g000078
rosa_roxburghii Rroxscaffold_7G00190180
rosa_rugosa Rorug06G0118400
rosa_samantha Rh6DG225700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 171
AccI GTMKAC 1 cut(s) 564
AccIII TCCGGA 1 cut(s) 762
AcoI YGGCCR 1 cut(s) 259
AfaI GTAC 1 cut(s) 99
AfiI CCNNNNNNNGG 2 cut(s) 257, 639
AgsI TTSAA 5 cut(s) 67, 328, 392, 478, 663
AjnI CCWGG 3 cut(s) 22, 494, 638
AjuI GAANNNNNNNTTGG 2 cut(s) 584, 616
AleI CACNNNNGTG 1 cut(s) 158
AluBI AGCT 5 cut(s) 43, 88, 417, 595, 688
AluI AGCT 5 cut(s) 43, 88, 417, 595, 688
Alw26I GTCTC 2 cut(s) 222, 314
Aor13HI TCCGGA 1 cut(s) 762
AoxI GGCC 1 cut(s) 259
ApeKI GCWGC 1 cut(s) 275
AsuHPI GGTGA 1 cut(s) 326
BanI GGYRCC 1 cut(s) 171
BbsI GAAGAC 1 cut(s) 291
BbvI GCAGC 1 cut(s) 262
BccI CCATC 2 cut(s) 698, 719
BciT130I CCWGG 3 cut(s) 24, 496, 640
BcoDI GTCTC 2 cut(s) 222, 314
BfaI CTAG 2 cut(s) 44, 627
BfmI CTRYAG 1 cut(s) 462
BglI GCCNNNNNGGC 1 cut(s) 267
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
Bme1390I CCNGG 3 cut(s) 24, 496, 640
BmiI GGNNCC 3 cut(s) 28, 173, 709
BmrFI CCNGG 3 cut(s) 24, 496, 640
BmsI GCATC 2 cut(s) 130, 517
BpiI GAAGAC 1 cut(s) 291
BplI GAGNNNNNCTC 2 cut(s) 219, 251
BpmI CTGGAG 2 cut(s) 141, 246
BsaJI CCNNGG 2 cut(s) 176, 262
BsaWI WCCGGW 2 cut(s) 333, 762
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 639
Bse1I ACTGG 1 cut(s) 158
BseAI TCCGGA 1 cut(s) 762
BseBI CCWGG 3 cut(s) 24, 496, 640
BseDI CCNNGG 2 cut(s) 176, 262
BseGI GGATG 3 cut(s) 145, 711, 757
BseLI CCNNNNNNNGG 2 cut(s) 257, 639
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 1 cut(s) 158
BseXI GCAGC 1 cut(s) 262
BseYI CCCAGC 1 cut(s) 417
BshFI GGCC 1 cut(s) 261
BshNI GGYRCC 1 cut(s) 171
BsiSI CCGG 2 cut(s) 334, 763
BslFI GGGAC 1 cut(s) 435
BslI CCNNNNNNNGG 2 cut(s) 257, 639
BsmAI GTCTC 2 cut(s) 222, 314
BsmBI CGTCTC 1 cut(s) 222
BsmFI GGGAC 1 cut(s) 435
BsnI GGCC 1 cut(s) 261
Bsp13I TCCGGA 1 cut(s) 762
Bsp143I GATC 1 cut(s) 769
BspANI GGCC 1 cut(s) 261
BspCNI CTCAG 1 cut(s) 73
BspEI TCCGGA 1 cut(s) 762
BspLI GGNNCC 3 cut(s) 28, 173, 709
BspQI GCTCTTC 1 cut(s) 537
BspT107I GGYRCC 1 cut(s) 171
BsrI ACTGG 1 cut(s) 158
BssECI CCNNGG 2 cut(s) 176, 262
BssMI GATC 1 cut(s) 769
BssT1I CCWWGG 2 cut(s) 176, 262
Bst2UI CCWGG 3 cut(s) 24, 496, 640
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 1 cut(s) 537
BstC8I GCNNGC 2 cut(s) 86, 415
BstDEI CTNAG 2 cut(s) 60, 505
BstF5I GGATG 3 cut(s) 145, 711, 757
BstKTI GATC 1 cut(s) 772
BstMAI GTCTC 2 cut(s) 222, 314
BstMBI GATC 1 cut(s) 769
BstMWI GCNNNNNNNGC 5 cut(s) 230, 267, 500, 509, 592
BstNI CCWGG 3 cut(s) 24, 496, 640
BstSCI CCNGG 3 cut(s) 22, 494, 638
BstSFI CTRYAG 1 cut(s) 462
BstV1I GCAGC 1 cut(s) 262
BstV2I GAAGAC 1 cut(s) 291
BstXI CCANNNNNNTGG 1 cut(s) 23
BsuRI GGCC 1 cut(s) 261
BtsCI GGATG 3 cut(s) 145, 711, 757
BtsIMutI CAGTG 2 cut(s) 129, 165
Cac8I GCNNGC 2 cut(s) 86, 415
CseI GACGC 1 cut(s) 239
CsiI ACCWGGT 1 cut(s) 638
Csp6I GTAC 1 cut(s) 98
CviAII CATG 4 cut(s) 244, 444, 483, 569
CviQI GTAC 1 cut(s) 98
DdeI CTNAG 2 cut(s) 60, 505
DpnI GATC 1 cut(s) 771
DpnII GATC 1 cut(s) 769
EaeI YGGCCR 1 cut(s) 259
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Eco130I CCWWGG 2 cut(s) 176, 262
EcoRII CCWGG 3 cut(s) 22, 494, 638
EcoT14I CCWWGG 2 cut(s) 176, 262
ErhI CCWWGG 2 cut(s) 176, 262
Esp3I CGTCTC 1 cut(s) 222
FaeI CATG 4 cut(s) 247, 447, 486, 572
FaqI GGGAC 1 cut(s) 435
FatI CATG 4 cut(s) 243, 443, 482, 568
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 3 cut(s) 152, 698, 764
Fsp4HI GCNGC 1 cut(s) 276
FspBI CTAG 2 cut(s) 44, 627
GluI GCNGC 1 cut(s) 276
GsaI CCCAGC 1 cut(s) 421
GsuI CTGGAG 2 cut(s) 141, 246
HaeIII GGCC 1 cut(s) 261
HapII CCGG 2 cut(s) 334, 763
HgaI GACGC 1 cut(s) 239
Hin1II CATG 4 cut(s) 247, 447, 486, 572
HindIII AAGCTT 1 cut(s) 686
HinfI GANTC 5 cut(s) 423, 440, 605, 671, 729
HpaII CCGG 2 cut(s) 334, 763
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 2 cut(s) 34, 92
Hpy188III TCNNGA 1 cut(s) 763
Hpy8I GTNNAC 1 cut(s) 565
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 1 cut(s) 16
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4V TGCA 3 cut(s) 278, 586, 614
HpyF10VI GCNNNNNNNGC 5 cut(s) 230, 267, 500, 509, 592
HpyF3I CTNAG 2 cut(s) 60, 505
Hsp92II CATG 4 cut(s) 247, 447, 486, 572
Kpn2I TCCGGA 1 cut(s) 762
Kzo9I GATC 1 cut(s) 769
LguI GCTCTTC 1 cut(s) 537
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 2 cut(s) 130, 517
MabI ACCWGGT 1 cut(s) 638
MaeI CTAG 2 cut(s) 44, 627
MalI GATC 1 cut(s) 771
MboI GATC 1 cut(s) 769
MboII GAAGA 6 cut(s) 61, 296, 340, 374, 524, 680
MluCI AATT 4 cut(s) 134, 345, 406, 544
MlyI GAGTC 2 cut(s) 417, 738
MmeI TCCRAC 3 cut(s) 417, 582, 757
MnlI CCTC 7 cut(s) 40, 310, 330, 367, 378, 396, 450
MroI TCCGGA 1 cut(s) 762
MseI TTAA 3 cut(s) 684, 717, 723
MslI CAYNNNNRTG 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 417
MspI CCGG 2 cut(s) 334, 763
MspR9I CCNGG 3 cut(s) 24, 496, 640
MvaI CCWGG 3 cut(s) 24, 496, 640
MwoI GCNNNNNNNGC 5 cut(s) 230, 267, 500, 509, 592
NdeII GATC 1 cut(s) 769
NlaIII CATG 4 cut(s) 247, 447, 486, 572
NlaIV GGNNCC 3 cut(s) 28, 173, 709
OliI CACNNNNGTG 1 cut(s) 158
PciSI GCTCTTC 1 cut(s) 537
PfeI GAWTC 3 cut(s) 440, 605, 671
PkrI GCNGC 1 cut(s) 277
PleI GAGTC 2 cut(s) 417, 737
PpsI GAGTC 2 cut(s) 417, 737
Psp6I CCWGG 3 cut(s) 22, 494, 638
PspFI CCCAGC 1 cut(s) 417
PspGI CCWGG 3 cut(s) 22, 494, 638
PspN4I GGNNCC 3 cut(s) 28, 173, 709
PvuII CAGCTG 1 cut(s) 417
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
RseI CAYNNNNRTG 1 cut(s) 158
SapI GCTCTTC 1 cut(s) 537
SaqAI TTAA 3 cut(s) 684, 717, 723
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 1 cut(s) 769
SchI GAGTC 2 cut(s) 417, 738
ScrFI CCNGG 3 cut(s) 24, 496, 640
SexAI ACCWGGT 1 cut(s) 638
SfaNI GCATC 2 cut(s) 130, 517
SfcI CTRYAG 1 cut(s) 462
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 4 cut(s) 134, 345, 406, 544
SspI AATATT 1 cut(s) 199
SspMI CTAG 2 cut(s) 44, 627
StyD4I CCNGG 3 cut(s) 22, 494, 638
StyI CCWWGG 2 cut(s) 176, 262
TaaI ACNGT 1 cut(s) 290
TaqI TCGA 3 cut(s) 9, 426, 666
TasI AATT 4 cut(s) 134, 345, 406, 544
TfiI GAWTC 3 cut(s) 440, 605, 671
Tru1I TTAA 3 cut(s) 684, 717, 723
Tru9I TTAA 3 cut(s) 684, 717, 723
TscAI CASTG 2 cut(s) 136, 165
TseI GCWGC 1 cut(s) 275
TspDTI ATGAA 4 cut(s) 120, 375, 585, 633
TspRI CASTG 2 cut(s) 136, 165
XmiI GTMKAC 1 cut(s) 564
XspI CTAG 2 cut(s) 44, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.