Rh7BG085000

Protein of unknown function (DUF2439)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012619
Physical Location & Seq
Forward (+)
6177910 .. 6186384
8475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG085000.1

Sequence Viewer

Length: 2139 bp
ATGGCGGAAGTGAAGCGGTGGAGTGTGACCTACACTAAGCACATGAAGCAGAAGCGCAAGGTTTACCAGGACGGATTCTTAGAGCTCCACACCTCCACCAATAAGATGTTGCTGTTCGACGACTGCGAGAAGCTCTTGGAGTGCAGGATCTTGAAGAAAGACGAAGCCGTTAGCTCCGGCGAAACACTCACATTCAATGCCTTTCTGGTTGATGTGAACGACGCTGAATCCGGTGACGGAGATCAGAAACCTAAACCTGAACCTGGTTTCAGTTCTCGAGAAAACGACATGAAGTTCAGTGAGAAACGAGCGAAATCGTGGAATAAGCCACAGCCGTTTCAGACTCCGGCGCAAAATATAATCAGAGGAAAGAAGCCACTGCAAAATTTGAGTCCATCGCAGAATATTATCAAAGAGTTCAAGAAGAGGGAACTACACAAGTATGGTGCAGCACTTAGTAGTCCAGAGACAGTGACAACTAGCACAACAGAATGGCAGGCTTTGCACACCACACAGCTAACTCAAAAGGCGAAGAAGTATCATGATGGTTTCTTGCAACTAGCAATTACTGGGTCTCATGGAAAGCAGATAATTCTGTTGGATTCAAGCAGGAATCAGTTAGATAACAGGTTCCTTAAGAAAGATGAAGTCATAGCATCCGGTGGATCAATAGCATTTGATGCTCATTTGGTTGATATTGGAGAGCAGGAAGGAGACCATAAACCTACGGATTTGCATCGTAAAGAAAATAAGCTGAATATTGTGGGAGAGGCAAGCATAAAGCAGGAACAGAGTAATGGTGTTATCACTGGTAAAGATGATGGAAAAGAATGGAAGGTCCTATACACTACACAAGTAACTCAAAAGGCCAAGAAGTACCATGATGGCTTCTTACAGATCACATCTAGTGGCTCCTTTGGAAGACAGGCGATGCTATTTGATGCAACTAGGAAACTCTTGGAAACCAGGTTCCTAAAAAAAGATGAAGTTATAAGCTCTGGTGAATCATTTGCATTTAATGCTCATTTGGTTGATGTCGGGGAACCTGAAGGGAATGAAGCTCAGGTTGATTTGAAGTCTCAAGGAAAAGATTGCCATGTGCAGAAATCAGGGATATTGCATAGAGAAGATGATTGCATTATAATCGATGATTTTGAACTAAAAGAGTGGGAAGCTCTATACACCACTCAGATCACTCAGAAGTCCAAGAAGTACCATGTTGGCATCCTCAGACTTGCCTCTTCTGGTTCTTTCCGTAAGAAGGTTACTTTACTTGGTGAAGATAAAACCATACTGAGTAATAAGTATGTTAGTTTGTCAGAAGATATAAAAACAGGATCTACTCTTGAGTTACCAAAATACTTGGTGGAGGTTGGTATTCCACATGCAATTGCTGGAGGTGAAGGAACAACTCTCAACAAAGCTTGTATAAAGAAACCTTCAGACTCAAGGTTTAGCATCTCTAGTGTAGAGGAAACAAAATTAAGCAGAGGAGTCTCCAGAAACAACCCTTTATGTGATGGTGAAGAAAACTCTCAGAACAAAGCTTGTTCGAAGAAAGACTTAAACTCAAGTTTTAGCATCTCTAGTGTAGAGGAAACAAAACCAAGCAGGGGAGTCCCTACAAACAAGCTTCTACGTGATGCCCATCAGATTTTGTCCTTTCTCCAAAACCCTATGGCTCAGGAGAGTGTAGCTTCACGTCAAGCTAACAGCACTGAAAAACCAGTTTCCTCAACTAAGGGGTCTCAAGTTTCAGATGCTATAATGGTGGATATCTCTGAAGATGTTCGGCTAGCAGAATCACAATCATCAGAACCACATAACAGATCAGTTGAAAATAAAGAACCAGGCAATATTATGGAAATTGGAATTAAAGCAATGTCCAGCGGTTGTGGCAGTCAATTAGCTGAGGGTATAGACATCAAAAACTCCCACCAGCTTCATTCAGACCATGTTGAAGCCGACACTGCACGTCATAACAGAGAATGTGCTTCTGATGAATCAAGATCAAGTTCTTGTTTTTCTCATGATGCTGCTGACGACGAGAAGATTGTATCCCAGGAGCCTAGTTGTGCAAGGAACCAAGAAAGCCCAAGTTTTGAACTAGAATTCCCTAGTTTTGATCTTGGATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2439

Protein Analysis

712

Amino Acids

79.14

Weight (kDa)

6.23

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZGRF1-like_N PF10382 4 - 76 6.3e-15 DNA helicase ZGRF1-like, N-terminal domain
ZGRF1-like_N PF10382 163 - 239 9e-13 DNA helicase ZGRF1-like, N-terminal domain
ZGRF1-like_N PF10382 276 - 354 8.4e-17 DNA helicase ZGRF1-like, N-terminal domain
ZGRF1-like_N PF10382 388 - 459 7.3e-13 DNA helicase ZGRF1-like, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 992, 1142
AciI CCGC 3 cut(s) 5, 16, 1890
AclWI GGATC 3 cut(s) 155, 673, 1345
AcsI RAATTY 2 cut(s) 385, 2111
AcuI CTGAAG 3 cut(s) 1068, 1425, 1803
AfaI GTAC 2 cut(s) 878, 1214
AfiI CCNNNNNNNGG 3 cut(s) 263, 1261, 1613
AflII CTTAAG 1 cut(s) 635
AgsI TTSAA 9 cut(s) 154, 196, 421, 606, 1075, 1157, 1838, 1961, 2105
AjiI CACGTC 2 cut(s) 1703, 1976
AjnI CCWGG 5 cut(s) 66, 262, 965, 1849, 2061
AloI GAACNNNNNNTCC 4 cut(s) 953, 985, 2097, 2129
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 6 cut(s) 461, 579, 708, 1083, 1501, 1752
AlwI GGATC 3 cut(s) 155, 673, 1345
Ama87I CYCGRG 1 cut(s) 276
AoxI GGCC 1 cut(s) 867
ApeKI GCWGC 2 cut(s) 449, 2036
ApoI RAATTY 2 cut(s) 385, 2111
ArsI GACNNNNNNTTYG 2 cut(s) 376, 408
Asp700I GAANNNNTTC 1 cut(s) 1788
AspLEI GCGC 2 cut(s) 57, 352
AspS9I GGNCC 1 cut(s) 838
AsuHPI GGTGA 5 cut(s) 245, 1013, 1289, 1412, 1535
AsuII TTCGAA 1 cut(s) 1553
AsuNHI GCTAGC 1 cut(s) 1795
AvaI CYCGRG 1 cut(s) 276
AvaII GGWCC 1 cut(s) 838
BanII GRGCYC 1 cut(s) 87
BarI GAAGNNNNNNTAC 2 cut(s) 827, 859
BbsI GAAGAC 1 cut(s) 928
Bbv12I GWGCWC 1 cut(s) 87
BbvCI CCTCAGC 1 cut(s) 1911
BbvI GCAGC 2 cut(s) 461, 2023
BccI CCATC 6 cut(s) 403, 539, 815, 878, 1514, 1656
BceAI ACGGC 2 cut(s) 152, 319
BcgI CGANNNNNNTGC 2 cut(s) 1126, 1160
BciT130I CCWGG 5 cut(s) 68, 264, 967, 1851, 2063
BciVI GTATCC 1 cut(s) 2068
BcoDI GTCTC 6 cut(s) 461, 579, 708, 1083, 1501, 1752
BfrI CTTAAG 1 cut(s) 635
BfuI GTATCC 1 cut(s) 2068
BisI GCNGC 2 cut(s) 450, 2037
BlsI GCNGC 2 cut(s) 451, 2038
Bme1390I CCNGG 5 cut(s) 68, 264, 967, 1851, 2063
Bme18I GGWCC 1 cut(s) 838
BmeT110I CYCGRG 1 cut(s) 276
BmgBI CACGTC 2 cut(s) 1703, 1976
BmgT120I GGNCC 1 cut(s) 838
BmiI GGNNCC 6 cut(s) 632, 913, 971, 1044, 2067, 2084
BmrFI CCNGG 5 cut(s) 68, 264, 967, 1851, 2063
BmrI ACTGGG 1 cut(s) 579
BmtI GCTAGC 1 cut(s) 1799
BmuI ACTGGG 1 cut(s) 579
BpiI GAAGAC 1 cut(s) 928
BpmI CTGGAG 2 cut(s) 1416, 1483
Bpu10I CCTNAGC 3 cut(s) 1062, 1683, 1911
Bpu14I TTCGAA 1 cut(s) 1553
BpuEI CTTGAG 5 cut(s) 1065, 1367, 1432, 1555, 1734
Bsa29I ATCGAT 1 cut(s) 1146
BsaAI YACGTR 1 cut(s) 1640
BsaBI GATNNNNATC 2 cut(s) 735, 1647
BsaI GGTCTC 3 cut(s) 579, 708, 1752
BsaJI CCNNGG 1 cut(s) 2061
BsaWI WCCGGW 2 cut(s) 230, 659
Bsc4I CCNNNNNNNGG 3 cut(s) 263, 1261, 1613
Bse1I ACTGG 3 cut(s) 574, 814, 1727
Bse3DI GCAATG 1 cut(s) 1887
Bse8I GATNNNNATC 2 cut(s) 735, 1647
BseBI CCWGG 5 cut(s) 68, 264, 967, 1851, 2063
BseCI ATCGAT 1 cut(s) 1146
BseDI CCNNGG 1 cut(s) 2061
BseGI GGATG 2 cut(s) 656, 1224
BseJI GATNNNNATC 2 cut(s) 735, 1647
BseLI CCNNNNNNNGG 3 cut(s) 263, 1261, 1613
BseMI GCAATG 1 cut(s) 1887
BseMII CTCAG 8 cut(s) 1076, 1202, 1211, 1243, 1286, 1550, 1697, 1902
BseNI ACTGG 3 cut(s) 574, 814, 1727
BseRI GAGGAG 1 cut(s) 1506
BseXI GCAGC 2 cut(s) 461, 2023
BsgI GTGCAG 4 cut(s) 163, 468, 1121, 1956
BshFI GGCC 1 cut(s) 869
BshVI ATCGAT 1 cut(s) 1146
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 276
BsiSI CCGG 4 cut(s) 177, 231, 347, 660
BslFI GGGAC 1 cut(s) 1604
BslI CCNNNNNNNGG 3 cut(s) 263, 1261, 1613
BsmAI GTCTC 6 cut(s) 461, 579, 708, 1083, 1501, 1752
BsmFI GGGAC 1 cut(s) 1604
BsnI GGCC 1 cut(s) 869
Bso31I GGTCTC 3 cut(s) 579, 708, 1752
BsoBI CYCGRG 1 cut(s) 276
Bsp119I TTCGAA 1 cut(s) 1553
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 9 cut(s) 147, 241, 665, 897, 1191, 1337, 1829, 2009, 2125
BspACI CCGC 3 cut(s) 5, 16, 1890
BspANI GGCC 1 cut(s) 869
BspCNI CTCAG 8 cut(s) 1075, 1201, 1210, 1242, 1287, 1549, 1696, 1903
BspDI ATCGAT 1 cut(s) 1146
BspHI TCATGA 2 cut(s) 541, 2029
BspLI GGNNCC 6 cut(s) 632, 913, 971, 1044, 2067, 2084
BspOI GCTAGC 1 cut(s) 1799
BspPI GGATC 3 cut(s) 155, 673, 1345
BspT104I TTCGAA 1 cut(s) 1553
BspTI CTTAAG 1 cut(s) 635
BspTNI GGTCTC 3 cut(s) 579, 708, 1752
BsrDI GCAATG 1 cut(s) 1887
BsrI ACTGG 3 cut(s) 574, 814, 1727
BssECI CCNNGG 1 cut(s) 2061
BssMI GATC 9 cut(s) 147, 241, 665, 897, 1191, 1337, 1829, 2009, 2125
Bst2UI CCWGG 5 cut(s) 68, 264, 967, 1851, 2063
Bst4CI ACNGT 1 cut(s) 472
Bst6I CTCTTC 2 cut(s) 419, 1246
BstAFI CTTAAG 1 cut(s) 635
BstAPI GCANNNNNTGC 3 cut(s) 502, 680, 1019
BstBAI YACGTR 1 cut(s) 1640
BstBI TTCGAA 1 cut(s) 1553
BstC8I GCNNGC 3 cut(s) 498, 775, 1797
BstF5I GGATG 2 cut(s) 656, 1224
BstHHI GCGC 2 cut(s) 57, 352
BstKTI GATC 9 cut(s) 150, 244, 668, 900, 1194, 1340, 1832, 2012, 2128
BstMAI GTCTC 6 cut(s) 461, 579, 708, 1083, 1501, 1752
BstMBI GATC 9 cut(s) 147, 241, 665, 897, 1191, 1337, 1829, 2009, 2125
BstMWI GCNNNNNNNGC 6 cut(s) 46, 502, 680, 1019, 1896, 1970
BstNI CCWGG 5 cut(s) 68, 264, 967, 1851, 2063
BstNSI RCATGY 1 cut(s) 1388
BstSCI CCNGG 5 cut(s) 66, 262, 965, 1849, 2061
BstV1I GCAGC 2 cut(s) 461, 2023
BstV2I GAAGAC 1 cut(s) 928
BstX2I RGATCY 2 cut(s) 147, 1337
BstYI RGATCY 2 cut(s) 147, 1337
Bsu15I ATCGAT 1 cut(s) 1146
BsuI GTATCC 1 cut(s) 2068
BsuRI GGCC 1 cut(s) 869
BsuTUI ATCGAT 1 cut(s) 1146
BtgZI GCGATG 2 cut(s) 381, 944
BtrI CACGTC 2 cut(s) 1703, 1976
BtsCI GGATG 2 cut(s) 656, 1224
BtsI GCAGTG 2 cut(s) 377, 1968
BtsIMutI CAGTG 6 cut(s) 304, 377, 477, 807, 1716, 1968
Cac8I GCNNGC 3 cut(s) 498, 775, 1797
CciI TCATGA 2 cut(s) 541, 2029
CfoI GCGC 2 cut(s) 57, 352
Cfr13I GGNCC 1 cut(s) 838
ClaI ATCGAT 1 cut(s) 1146
CseI GACGC 1 cut(s) 230
CsiI ACCWGGT 2 cut(s) 262, 965
Csp6I GTAC 2 cut(s) 877, 1213
CviQI GTAC 2 cut(s) 877, 1213
DpnI GATC 9 cut(s) 149, 243, 667, 899, 1193, 1339, 1831, 2011, 2127
DpnII GATC 9 cut(s) 147, 241, 665, 897, 1191, 1337, 1829, 2009, 2125
Eam1104I CTCTTC 2 cut(s) 419, 1246
EarI CTCTTC 2 cut(s) 419, 1246
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 87
Eco31I GGTCTC 3 cut(s) 579, 708, 1752
Eco32I GATATC 1 cut(s) 1777
Eco47I GGWCC 1 cut(s) 838
Eco53kI GAGCTC 1 cut(s) 85
Eco57I CTGAAG 3 cut(s) 1068, 1425, 1803
Eco88I CYCGRG 1 cut(s) 276
EcoICRI GAGCTC 1 cut(s) 85
EcoO109I RGGNCCY 1 cut(s) 838
EcoRI GAATTC 1 cut(s) 2111
EcoRII CCWGG 5 cut(s) 66, 262, 965, 1849, 2061
EcoRV GATATC 1 cut(s) 1777
EcoT38I GRGCYC 1 cut(s) 87
FalI AAGNNNNNCTT 2 cut(s) 1547, 1579
FaqI GGGAC 1 cut(s) 1604
Fnu4HI GCNGC 2 cut(s) 450, 2037
FokI GGATG 2 cut(s) 643, 1211
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 2 cut(s) 450, 2037
GlaI GCGC 2 cut(s) 56, 351
GluI GCNGC 2 cut(s) 450, 2037
GsuI CTGGAG 2 cut(s) 1416, 1483
HaeIII GGCC 1 cut(s) 869
HapII CCGG 4 cut(s) 177, 231, 347, 660
HgaI GACGC 1 cut(s) 230
HhaI GCGC 2 cut(s) 57, 352
Hin6I GCGC 2 cut(s) 55, 350
HinP1I GCGC 2 cut(s) 55, 350
HindIII AAGCTT 3 cut(s) 1422, 1545, 1631
HpaII CCGG 4 cut(s) 177, 231, 347, 660
HphI GGTGA 5 cut(s) 245, 1013, 1289, 1412, 1535
Hpy166II GTNNAC 2 cut(s) 64, 217
Hpy8I GTNNAC 2 cut(s) 64, 217
Hpy99I CGWCG 3 cut(s) 122, 224, 2048
HpyAV CCTTC 6 cut(s) 704, 829, 1043, 1255, 1397, 1449
HpyCH4III ACNGT 1 cut(s) 472
HpyCH4IV ACGT 3 cut(s) 1639, 1702, 1975
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 502, 680, 1019, 1896, 1970
HpySE526I ACGT 3 cut(s) 1639, 1702, 1975
HspAI GCGC 2 cut(s) 55, 350
Kzo9I GATC 9 cut(s) 147, 241, 665, 897, 1191, 1337, 1829, 2009, 2125
LmnI GCTCC 4 cut(s) 90, 179, 917, 2065
Lsp1109I GCAGC 2 cut(s) 461, 2023
MabI ACCWGGT 2 cut(s) 262, 965
MaeII ACGT 3 cut(s) 1639, 1702, 1975
MaeIII GTNAC 6 cut(s) 25, 233, 472, 856, 1264, 1350
MalI GATC 9 cut(s) 149, 243, 667, 899, 1193, 1339, 1831, 2011, 2127
MboI GATC 9 cut(s) 147, 241, 665, 897, 1191, 1337, 1829, 2009, 2125
MfeI CAATTG 1 cut(s) 1389
MflI RGATCY 2 cut(s) 147, 1337
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 9 cut(s) 385, 564, 591, 1389, 1481, 1866, 1872, 1904, 2111
MlyI GAGTC 5 cut(s) 337, 400, 1439, 1503, 1626
MmeI TCCRAC 1 cut(s) 579
MroXI GAANNNNTTC 1 cut(s) 1788
MseI TTAA 6 cut(s) 636, 1017, 1484, 1565, 1875, 2137
MslI CAYNNNNRTG 1 cut(s) 441
MspA1I CMGCKG 1 cut(s) 1890
MspCI CTTAAG 1 cut(s) 635
MspI CCGG 4 cut(s) 177, 231, 347, 660
MspR9I CCNGG 5 cut(s) 68, 264, 967, 1851, 2063
MunI CAATTG 1 cut(s) 1389
MvaI CCWGG 5 cut(s) 68, 264, 967, 1851, 2063
MwoI GCNNNNNNNGC 6 cut(s) 46, 502, 680, 1019, 1896, 1970
NdeII GATC 9 cut(s) 147, 241, 665, 897, 1191, 1337, 1829, 2009, 2125
NheI GCTAGC 1 cut(s) 1795
NlaIV GGNNCC 6 cut(s) 632, 913, 971, 1044, 2067, 2084
NmuCI GTSAC 3 cut(s) 25, 233, 472
NspI RCATGY 1 cut(s) 1388
NspV TTCGAA 1 cut(s) 1553
PaeR7I CTCGAG 1 cut(s) 276
PagI TCATGA 2 cut(s) 541, 2029
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 1788
PfeI GAWTC 7 cut(s) 75, 227, 602, 613, 1004, 1802, 2003
PkrI GCNGC 2 cut(s) 451, 2038
PleI GAGTC 5 cut(s) 337, 399, 1439, 1502, 1625
PpsI GAGTC 5 cut(s) 337, 399, 1439, 1502, 1625
Ppu21I YACGTR 1 cut(s) 1640
PpuMI RGGWCCY 1 cut(s) 838
PsiI TTATAA 2 cut(s) 992, 1142
Psp124BI GAGCTC 1 cut(s) 87
Psp5II RGGWCCY 1 cut(s) 838
Psp6I CCWGG 5 cut(s) 66, 262, 965, 1849, 2061
PspGI CCWGG 5 cut(s) 66, 262, 965, 1849, 2061
PspN4I GGNNCC 6 cut(s) 632, 913, 971, 1044, 2067, 2084
PspPI GGNCC 1 cut(s) 838
PspPPI RGGWCCY 1 cut(s) 838
PsuI RGATCY 2 cut(s) 147, 1337
RsaI GTAC 2 cut(s) 878, 1214
RsaNI GTAC 2 cut(s) 877, 1213
RseI CAYNNNNRTG 1 cut(s) 441
SacI GAGCTC 1 cut(s) 87
SaqAI TTAA 6 cut(s) 636, 1017, 1484, 1565, 1875, 2137
SatI GCNGC 2 cut(s) 450, 2037
Sau3AI GATC 9 cut(s) 147, 241, 665, 897, 1191, 1337, 1829, 2009, 2125
Sau96I GGNCC 1 cut(s) 838
SchI GAGTC 5 cut(s) 337, 400, 1439, 1503, 1626
ScrFI CCNGG 5 cut(s) 68, 264, 967, 1851, 2063
SduI GDGCHC 1 cut(s) 87
SexAI ACCWGGT 2 cut(s) 262, 965
Sfr274I CTCGAG 1 cut(s) 276
SfuI TTCGAA 1 cut(s) 1553
SinI GGWCC 1 cut(s) 838
SlaI CTCGAG 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 7 cut(s) 276, 635, 1080, 1346, 1447, 1570, 1749
SmoI CTYRAG 7 cut(s) 276, 635, 1080, 1346, 1447, 1570, 1749
Sse9I AATT 9 cut(s) 385, 564, 591, 1389, 1481, 1866, 1872, 1904, 2111
SsiI CCGC 3 cut(s) 5, 16, 1890
SspI AATATT 3 cut(s) 406, 760, 1858
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 5 cut(s) 66, 262, 965, 1849, 2061
TaaI ACNGT 1 cut(s) 472
TaiI ACGT 3 cut(s) 1642, 1705, 1978
TaqI TCGA 4 cut(s) 117, 277, 1146, 1553
TasI AATT 9 cut(s) 385, 564, 591, 1389, 1481, 1866, 1872, 1904, 2111
TfiI GAWTC 7 cut(s) 75, 227, 602, 613, 1004, 1802, 2003
Tru1I TTAA 6 cut(s) 636, 1017, 1484, 1565, 1875, 2137
Tru9I TTAA 6 cut(s) 636, 1017, 1484, 1565, 1875, 2137
TscAI CASTG 6 cut(s) 304, 384, 477, 814, 1723, 1975
TseFI GTSAC 3 cut(s) 25, 233, 472
TseI GCWGC 2 cut(s) 449, 2036
Tsp45I GTSAC 3 cut(s) 25, 233, 472
TspDTI ATGAA 7 cut(s) 59, 305, 660, 999, 1071, 1934, 2016
TspGWI ACGGA 4 cut(s) 87, 252, 743, 1244
TspRI CASTG 6 cut(s) 304, 384, 477, 814, 1723, 1975
Vha464I CTTAAG 1 cut(s) 635
VpaK11BI GGWCC 1 cut(s) 838
XapI RAATTY 2 cut(s) 385, 2111
XceI RCATGY 1 cut(s) 1388
XhoI CTCGAG 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 1788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.