Rh7BG308100

Ferric reduction oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0017311
Physical Location & Seq
Reverse (-)
29573760 .. 29578106
4347 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG308100.1

Sequence Viewer

Length: 1707 bp
ATGGCCGTGGACGATTTACACTCTGCTCTTATTTCCAATGGCGCCGGAGGTTATGCCAAGCAGAGCCAGCAGAGGAGCTTTCTTTTTTCACTGGCCAAATGGGTTCTCAAATCTGCAATGTGGGTCATATTCGTTGCCTGGATTGTTCTCTTCTTCATTATACCTTCCGACTTCGGCTCCACTTTCTATGCTGATTGGGTTGCTGCCACCGATGGGACTCTGTTTGGAGAGACTGGGAGTGTGTTGATGTTATACAGTGCTCCGATTATCGCTATTGCTTTTCTTGCTGTTCCCTATATCATCTTTTTCGAGGAAGATGAAGAAGAGTATCAAGAGAAGAAGAAGGCGAAGTTCAGCCTATGGACCTTTCCTGTTCTGGTGGATGGACCACTTGGAGTTGTTTCTGCCGCTGAAATGATTGGCATTATTCTCTTTATTGTGTTTGTTCTCTCGGCGCTGTATTTTTACACTGTAGCCAACTTTGAGATGTTACCTGACTATGGTGATGATTTGACCGCAGGAGAGAAAAGGGTTTTTATGTTGCAAATGTCGGCTTATTGCTTTGGTTTGATAGTCTTATCTTGCTTGGCATTCCTGTTTCTCCCAATTGCAAGGGGATCAATTCTTCTCCGCCTTATTGATATACCATTCGAGCATGCGACCAGATATCATGTATGGTTGGGGAATCTCATATTCTTCCTTGTTACTATGCATGGACTCTGCTATATGATTGTGTGGGCGATACGAGGCATCATTCCATCTGAAGTAGTAGAGTGGAAAAGTGATGGTGGTGCAAACTTGGCGGGAATTATTTGCTATGGAATCTTTCTGTTGATTTGGGTGACAACACTTCCTCCTGTGAGAAAGCGATATTTTGAGCTTTTTTACTATACCCATCAACTCTATGTTTTGTTTGTCATCTTCTTGGCCTTGCATATTGGTGGCGTCAATTTCAGCCAAGCTGCTGGAGGAATATTTCTTTTTATGCTGGATCGCTTTCTAAGGTTCTGCCAATCACGAAAGACTGTCAACATAGTTTCAGCAACATGCTTTCCATGTGGAACTGTCAAGTTGGTGCTTCCAAAACCTGCAAACTTGCACTACAATGCGCTTGGTTTCATCTTTCTTCAAGTGAAGAGTATATCTTCACTGCAATGGCATCCTTTCAGCGTCTCATCCAGTCCTCTGAATGGTAAATATCATCTTGAAGTCCTCATAAAGACCGCTGGGGAATGGACCACAAAGCTAAGGGAAACAGTCTCCAAGACTTCTGCAGAGGACTCAGATATACAGCTTCCACACACTCCTGCTCATCAGATTATGGCTTCTGTTGAGGGGCCTTATGGCCATGAATCACCGTACTACTTGAGGTATGAAAAACTTGTCTTGGTAGCAGGAGGCAGTGGGATCTCACCATTCTTAGCTATCTTGAGTGATATTCTCCATCGAATTAAGATGAACAAACCTTGTCTACCGCAACATGTGTTGCTTGTTTGGGCTATCAAAACGTCAAATGAGCTCTCTCTTCTTTCCACAGTTGACATGGGGTCTATGGATTTTTCTGATAGATTGAATATTGAAATCCAGGCCTATGTCACACGTGAATCCGAATGTCCAGTGGTAGGTCTAATTGTGCATGAATTCCCTCCCCATACGATCTGTAACTTATCTTTTAAGCATGATTGTAGCTTACACTTCATTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_8 Ferric_reduct NAD_binding_6

Protein Analysis

568

Amino Acids

63.86

Weight (kDa)

6.22

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferric_reduct PF01794 188 - 309 1.3e-22 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 342 - 451 1.1e-18 FAD-binding domain
NAD_binding_6 PF08030 458 - 539 3.8e-13 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017311)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g27140
rosa_chinensis RchiOBHm_Chr7g0218141
rosa_laevigata RLG00000002396
rosa_multiflora Rmu_co8413095.1_g000001 Rmu_sc0003627.1_g000011 Rmu_sc0031732.1_g000001
rosa_roxburghii Rroxscaffold_3G00241270
rosa_rugosa Rorug07G0174500
rosa_samantha Rh7AG317000 Rh7BG308100 Rh7CG333800 Rh7DG315800
rosa_wichuraiana Rw7G026820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1096
AccB1I GGYRCC 1 cut(s) 41
AccI GTMKAC 1 cut(s) 1471
AciI CCGC 6 cut(s) 408, 516, 631, 803, 1224, 1475
AclWI GGATC 3 cut(s) 625, 999, 1415
AcoI YGGCCR 3 cut(s) 3, 93, 1345
AcsI RAATTY 1 cut(s) 1640
AcuI CTGAAG 1 cut(s) 783
AcvI CACGTG 1 cut(s) 1601
AcyI GRCGYC 2 cut(s) 42, 945
AfaI GTAC 1 cut(s) 1361
AfiI CCNNNNNNNGG 4 cut(s) 213, 500, 1190, 1622
AflIII ACRYGT 2 cut(s) 1480, 1598
AgsI TTSAA 4 cut(s) 1130, 1208, 1573, 1580
AhdI GACNNNNNGTC 1 cut(s) 1546
AjnI CCWGG 2 cut(s) 137, 1584
AluBI AGCT 8 cut(s) 78, 880, 962, 1246, 1294, 1424, 1519, 1689
AluI AGCT 8 cut(s) 78, 880, 962, 1246, 1294, 1424, 1519, 1689
Alw21I GWGCWC 2 cut(s) 262, 1521
Alw26I GTCTC 3 cut(s) 224, 1177, 1264
AlwI GGATC 3 cut(s) 625, 999, 1415
AoxI GGCC 6 cut(s) 3, 93, 927, 1337, 1345, 1587
ApeKI GCWGC 2 cut(s) 203, 962
ApoI RAATTY 1 cut(s) 1640
AspLEI GCGC 3 cut(s) 44, 457, 1111
AspS9I GGNCC 4 cut(s) 363, 386, 1236, 1337
AsuHPI GGTGA 4 cut(s) 515, 853, 1347, 1404
AvaII GGWCC 3 cut(s) 363, 386, 1236
BalI TGGCCA 2 cut(s) 95, 1347
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 1 cut(s) 1521
BbrPI CACGTG 1 cut(s) 1601
Bbv12I GWGCWC 2 cut(s) 262, 1521
BbvI GCAGC 2 cut(s) 190, 949
BccI CCATC 6 cut(s) 206, 377, 766, 779, 903, 1452
BciT130I CCWGG 2 cut(s) 139, 1586
BcoDI GTCTC 3 cut(s) 224, 1177, 1264
BfmI CTRYAG 2 cut(s) 471, 1272
BfoI RGCGCY 2 cut(s) 45, 458
BfuAI ACCTGC 1 cut(s) 1096
BisI GCNGC 3 cut(s) 204, 408, 963
BlsI GCNGC 3 cut(s) 205, 409, 964
Bme1390I CCNGG 2 cut(s) 139, 1586
Bme18I GGWCC 3 cut(s) 363, 386, 1236
BmeRI GACNNNNNGTC 1 cut(s) 1546
BmgT120I GGNCC 4 cut(s) 363, 386, 1236, 1337
BmiI GGNNCC 3 cut(s) 43, 178, 1338
BmrFI CCNGG 2 cut(s) 139, 1586
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 2 cut(s) 759, 1168
BmuI ACTGGG 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 987
Bpu10I CCTNAGC 1 cut(s) 1247
BpuEI CTTGAG 2 cut(s) 1387, 1450
BsaAI YACGTR 1 cut(s) 1601
BsaHI GRCGYC 2 cut(s) 42, 945
BsaJI CCNNGG 1 cut(s) 6
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 838, 868
Bsc4I CCNNNNNNNGG 4 cut(s) 213, 500, 1190, 1622
Bse1I ACTGG 4 cut(s) 96, 238, 1179, 1616
Bse3DI GCAATG 2 cut(s) 123, 1160
BseBI CCWGG 2 cut(s) 139, 1586
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 3 cut(s) 388, 1159, 1175
BseLI CCNNNNNNNGG 4 cut(s) 213, 500, 1190, 1622
BseMI GCAATG 2 cut(s) 123, 1160
BseMII CTCAG 1 cut(s) 1296
BseNI ACTGG 4 cut(s) 96, 238, 1179, 1616
BseRI GAGGAG 1 cut(s) 88
BseXI GCAGC 2 cut(s) 190, 949
BseYI CCCAGC 1 cut(s) 1226
BshFI GGCC 6 cut(s) 5, 95, 929, 1339, 1347, 1589
BshNI GGYRCC 1 cut(s) 41
BsiHKAI GWGCWC 2 cut(s) 262, 1521
BsiSI CCGG 1 cut(s) 45
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 4 cut(s) 213, 500, 1190, 1622
BsmAI GTCTC 3 cut(s) 224, 1177, 1264
BsmBI CGTCTC 1 cut(s) 1177
BsmFI GGGAC 1 cut(s) 229
BsmI GAATGC 1 cut(s) 590
BsnI GGCC 6 cut(s) 5, 95, 929, 1339, 1347, 1589
Bsp1286I GDGCHC 2 cut(s) 262, 1521
Bsp143I GATC 4 cut(s) 617, 991, 1407, 1656
BspACI CCGC 6 cut(s) 408, 516, 631, 803, 1224, 1475
BspANI GGCC 6 cut(s) 5, 95, 929, 1339, 1347, 1589
BspCNI CTCAG 1 cut(s) 1295
BspLI GGNNCC 3 cut(s) 43, 178, 1338
BspMAI CTGCAG 1 cut(s) 1276
BspMI ACCTGC 1 cut(s) 1096
BspPI GGATC 3 cut(s) 625, 999, 1415
BspT107I GGYRCC 1 cut(s) 41
BsrDI GCAATG 2 cut(s) 123, 1160
BsrI ACTGG 4 cut(s) 96, 238, 1179, 1616
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 4 cut(s) 617, 991, 1407, 1656
BssNI GRCGYC 2 cut(s) 42, 945
Bst2UI CCWGG 2 cut(s) 139, 1586
Bst4CI ACNGT 7 cut(s) 257, 472, 1027, 1066, 1258, 1359, 1537
Bst6I CTCTTC 4 cut(s) 155, 318, 1130, 1530
BstACI GRCGYC 2 cut(s) 42, 945
BstBAI YACGTR 1 cut(s) 1601
BstC8I GCNNGC 2 cut(s) 68, 657
BstDEI CTNAG 4 cut(s) 1001, 1247, 1282, 1420
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 3 cut(s) 388, 1159, 1175
BstH2I RGCGCY 2 cut(s) 45, 458
BstHHI GCGC 3 cut(s) 44, 457, 1111
BstKTI GATC 4 cut(s) 620, 994, 1410, 1659
BstMAI GTCTC 3 cut(s) 224, 1177, 1264
BstMBI GATC 4 cut(s) 617, 991, 1407, 1656
BstMWI GCNNNNNNNGC 3 cut(s) 67, 284, 800
BstNI CCWGG 2 cut(s) 139, 1586
BstNSI RCATGY 3 cut(s) 659, 1050, 1484
BstSCI CCNGG 2 cut(s) 137, 1584
BstSFI CTRYAG 2 cut(s) 471, 1272
BstV1I GCAGC 2 cut(s) 190, 949
BstX2I RGATCY 1 cut(s) 1407
BstXI CCANNNNNNTGG 1 cut(s) 965
BstYI RGATCY 1 cut(s) 1407
BsuRI GGCC 6 cut(s) 5, 95, 929, 1339, 1347, 1589
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 3 cut(s) 388, 1159, 1175
BtsI GCAGTG 2 cut(s) 1148, 1408
BtsIMutI CAGTG 6 cut(s) 89, 262, 468, 1148, 1408, 1623
BveI ACCTGC 1 cut(s) 1096
Cac8I GCNNGC 2 cut(s) 68, 657
CfoI GCGC 3 cut(s) 44, 457, 1111
Cfr13I GGNCC 4 cut(s) 363, 386, 1236, 1337
CseI GACGC 2 cut(s) 934, 1159
Csp6I GTAC 1 cut(s) 1360
CviQI GTAC 1 cut(s) 1360
DdeI CTNAG 4 cut(s) 1001, 1247, 1282, 1420
DinI GGCGCC 1 cut(s) 43
DpnI GATC 4 cut(s) 619, 993, 1409, 1658
DpnII GATC 4 cut(s) 617, 991, 1407, 1656
DriI GACNNNNNGTC 1 cut(s) 1546
EaeI YGGCCR 3 cut(s) 3, 93, 1345
Eam1104I CTCTTC 4 cut(s) 155, 318, 1130, 1530
Eam1105I GACNNNNNGTC 1 cut(s) 1546
EarI CTCTTC 4 cut(s) 155, 318, 1130, 1530
EciI GGCGGA 1 cut(s) 620
Ecl136II GAGCTC 1 cut(s) 1519
Eco147I AGGCCT 1 cut(s) 1589
Eco24I GRGCYC 1 cut(s) 1521
Eco32I GATATC 1 cut(s) 668
Eco47I GGWCC 3 cut(s) 363, 386, 1236
Eco53kI GAGCTC 1 cut(s) 1519
Eco57I CTGAAG 1 cut(s) 783
Eco72I CACGTG 1 cut(s) 1601
EcoICRI GAGCTC 1 cut(s) 1519
EcoO109I RGGNCCY 1 cut(s) 1337
EcoRI GAATTC 1 cut(s) 1640
EcoRII CCWGG 2 cut(s) 137, 1584
EcoRV GATATC 1 cut(s) 668
EcoT22I ATGCAT 1 cut(s) 714
EcoT38I GRGCYC 1 cut(s) 1521
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
Esp3I CGTCTC 1 cut(s) 1177
FaqI GGGAC 1 cut(s) 229
FauI CCCGC 1 cut(s) 796
FblI GTMKAC 1 cut(s) 1471
Fnu4HI GCNGC 3 cut(s) 204, 408, 963
FokI GGATG 3 cut(s) 395, 1146, 1162
FriOI GRGCYC 1 cut(s) 1521
Fsp4HI GCNGC 3 cut(s) 204, 408, 963
GlaI GCGC 3 cut(s) 43, 456, 1110
GluI GCNGC 3 cut(s) 204, 408, 963
GsaI CCCAGC 1 cut(s) 1230
GsuI CTGGAG 1 cut(s) 987
HaeII RGCGCY 2 cut(s) 45, 458
HaeIII GGCC 6 cut(s) 5, 95, 929, 1339, 1347, 1589
HapII CCGG 1 cut(s) 45
HgaI GACGC 2 cut(s) 934, 1159
HhaI GCGC 3 cut(s) 44, 457, 1111
Hin1I GRCGYC 2 cut(s) 42, 945
Hin6I GCGC 3 cut(s) 42, 455, 1109
HinP1I GCGC 3 cut(s) 42, 455, 1109
HincII GTYRAC 2 cut(s) 1030, 1540
HindII GTYRAC 2 cut(s) 1030, 1540
HinfI GANTC 7 cut(s) 217, 685, 717, 822, 1280, 1352, 1604
HpaII CCGG 1 cut(s) 45
HphI GGTGA 4 cut(s) 515, 853, 1347, 1404
Hpy166II GTNNAC 4 cut(s) 10, 1030, 1472, 1540
Hpy188I TCNGA 8 cut(s) 169, 264, 763, 1188, 1285, 1317, 1564, 1609
Hpy188III TCNNGA 4 cut(s) 332, 1017, 1205, 1429
Hpy8I GTNNAC 4 cut(s) 10, 1030, 1472, 1540
HpyAV CCTTC 2 cut(s) 174, 337
HpyCH4III ACNGT 7 cut(s) 257, 472, 1027, 1066, 1258, 1359, 1537
HpyCH4IV ACGT 2 cut(s) 1508, 1600
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 284, 800
HpyF3I CTNAG 4 cut(s) 1001, 1247, 1282, 1420
HpySE526I ACGT 2 cut(s) 1508, 1600
Hsp92I GRCGYC 2 cut(s) 42, 945
HspAI GCGC 3 cut(s) 42, 455, 1109
KasI GGCGCC 1 cut(s) 41
Kzo9I GATC 4 cut(s) 617, 991, 1407, 1656
LmnI GCTCC 3 cut(s) 75, 182, 265
Lsp1109I GCAGC 2 cut(s) 190, 949
LweI GCATC 2 cut(s) 759, 1168
MaeII ACGT 2 cut(s) 1508, 1600
MaeIII GTNAC 5 cut(s) 489, 703, 841, 1594, 1661
MalI GATC 4 cut(s) 619, 993, 1409, 1658
MboI GATC 4 cut(s) 617, 991, 1407, 1656
MfeI CAATTG 1 cut(s) 606
MflI RGATCY 1 cut(s) 1407
MhlI GDGCHC 2 cut(s) 262, 1521
MlsI TGGCCA 2 cut(s) 95, 1347
MluCI AATT 7 cut(s) 606, 621, 807, 949, 1449, 1629, 1640
MluNI TGGCCA 2 cut(s) 95, 1347
Mly113I GGCGCC 1 cut(s) 42
MlyI GAGTC 3 cut(s) 211, 711, 1274
MmeI TCCRAC 1 cut(s) 192
Mox20I TGGCCA 2 cut(s) 95, 1347
Mph1103I ATGCAT 1 cut(s) 714
MscI TGGCCA 2 cut(s) 95, 1347
MseI TTAA 2 cut(s) 1452, 1674
MslI CAYNNNNRTG 3 cut(s) 939, 1104, 1153
Msp20I TGGCCA 2 cut(s) 95, 1347
MspA1I CMGCKG 2 cut(s) 410, 1226
MspI CCGG 1 cut(s) 45
MspR9I CCNGG 2 cut(s) 139, 1586
MunI CAATTG 1 cut(s) 606
Mva1269I GAATGC 1 cut(s) 590
MvaI CCWGG 2 cut(s) 139, 1586
MwoI GCNNNNNNNGC 3 cut(s) 67, 284, 800
NarI GGCGCC 1 cut(s) 42
NdeII GATC 4 cut(s) 617, 991, 1407, 1656
NlaIV GGNNCC 3 cut(s) 43, 178, 1338
NmeAIII GCCGAG 1 cut(s) 431
NmuCI GTSAC 2 cut(s) 841, 1594
NsiI ATGCAT 1 cut(s) 714
NspI RCATGY 3 cut(s) 659, 1050, 1484
PaeI GCATGC 1 cut(s) 659
PceI AGGCCT 1 cut(s) 1589
PciI ACATGT 1 cut(s) 1480
PctI GAATGC 1 cut(s) 590
PfeI GAWTC 4 cut(s) 685, 822, 1352, 1604
PkrI GCNGC 3 cut(s) 205, 409, 964
PleI GAGTC 3 cut(s) 211, 711, 1274
PluTI GGCGCC 1 cut(s) 45
PmaCI CACGTG 1 cut(s) 1601
PmlI CACGTG 1 cut(s) 1601
PpsI GAGTC 3 cut(s) 211, 711, 1274
Ppu21I YACGTR 1 cut(s) 1601
PscI ACATGT 1 cut(s) 1480
Psp124BI GAGCTC 1 cut(s) 1521
Psp6I CCWGG 2 cut(s) 137, 1584
PspCI CACGTG 1 cut(s) 1601
PspFI CCCAGC 1 cut(s) 1226
PspGI CCWGG 2 cut(s) 137, 1584
PspN4I GGNNCC 3 cut(s) 43, 178, 1338
PspPI GGNCC 4 cut(s) 363, 386, 1236, 1337
PstI CTGCAG 1 cut(s) 1276
PsuI RGATCY 1 cut(s) 1407
RsaI GTAC 1 cut(s) 1361
RsaNI GTAC 1 cut(s) 1360
RseI CAYNNNNRTG 3 cut(s) 939, 1104, 1153
SacI GAGCTC 1 cut(s) 1521
SaqAI TTAA 2 cut(s) 1452, 1674
SatI GCNGC 3 cut(s) 204, 408, 963
Sau3AI GATC 4 cut(s) 617, 991, 1407, 1656
Sau96I GGNCC 4 cut(s) 363, 386, 1236, 1337
SchI GAGTC 3 cut(s) 211, 711, 1274
ScrFI CCNGG 2 cut(s) 139, 1586
SduI GDGCHC 2 cut(s) 262, 1521
SfaNI GCATC 2 cut(s) 759, 1168
SfcI CTRYAG 2 cut(s) 471, 1272
SfoI GGCGCC 1 cut(s) 43
SinI GGWCC 3 cut(s) 363, 386, 1236
SmiMI CAYNNNNRTG 3 cut(s) 939, 1104, 1153
SmlI CTYRAG 2 cut(s) 1366, 1429
SmoI CTYRAG 2 cut(s) 1366, 1429
SphI GCATGC 1 cut(s) 659
Sse9I AATT 7 cut(s) 606, 621, 807, 949, 1449, 1629, 1640
SseBI AGGCCT 1 cut(s) 1589
SsiI CCGC 6 cut(s) 408, 516, 631, 803, 1224, 1475
SspDI GGCGCC 1 cut(s) 41
SspI AATATT 2 cut(s) 975, 1576
SstI GAGCTC 1 cut(s) 1521
StuI AGGCCT 1 cut(s) 1589
StyD4I CCNGG 2 cut(s) 137, 1584
TaaI ACNGT 7 cut(s) 257, 472, 1027, 1066, 1258, 1359, 1537
TaiI ACGT 2 cut(s) 1511, 1603
TaqI TCGA 3 cut(s) 309, 651, 1447
TasI AATT 7 cut(s) 606, 621, 807, 949, 1449, 1629, 1640
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 4 cut(s) 685, 822, 1352, 1604
Tru1I TTAA 2 cut(s) 1452, 1674
Tru9I TTAA 2 cut(s) 1452, 1674
TscAI CASTG 6 cut(s) 96, 262, 475, 1155, 1408, 1623
TseFI GTSAC 2 cut(s) 841, 1594
TseI GCWGC 2 cut(s) 203, 962
Tsp45I GTSAC 2 cut(s) 841, 1594
TspDTI ATGAA 8 cut(s) 145, 333, 1108, 1365, 1389, 1472, 1653, 1687
TspRI CASTG 6 cut(s) 96, 262, 475, 1155, 1408, 1623
VpaK11BI GGWCC 3 cut(s) 363, 386, 1236
XapI RAATTY 1 cut(s) 1640
XceI RCATGY 3 cut(s) 659, 1050, 1484
XcmI CCANNNNNNNNNTGG 1 cut(s) 1540
XmiI GTMKAC 1 cut(s) 1471
Zsp2I ATGCAT 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.