Rw0G020750

transcription regulatory region sequence-specific DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00950
OG0017410
Physical Location & Seq
Forward (+)
10557 .. 11728
1172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G020750.1

Sequence Viewer

Length: 873 bp
ATGCCAAAGACTGCAAACCCGGGGAGGAGACATGCCACCTTGCTTAAGAAGGCCAATGAGTTATCTATTCTATGTGATGCAGAGGTCGCCATCATCGCTTTTTCACCTGATGGAGAACTCCATGAATACTCGAGCACAAGCATGGAACACACTCTTGAAAGATACAAGTACAGGACTGGCCTCGTCCTCCCAAGCAGAGTCGAGTCTCCGGCGCCAGAAGAGCACCGCCATGAACCTGCAAAGCAGGAACTGCAGGAAAGACATAAAGCGCTACGCTTGGAGAAATTGCAGAGAATGGGAAAGGAGTTGGATGGCCTGAGTTCGGACGCGCTGGCTACGCTTTTGGAACAACAAACTAAAGGGTTATTAGCTGTTCACAGCAAGAAAGTGGAACTGCTTGAGGAGAAGTTGCAGAGATCTAGGTCGAGAGAAGAGCAGGCTAAGCATGAGAATGGGCTGCTTGAGGAGAAGTTGCTGAGATCTAGGTCGAGAGAAGAGCAGACTATGCACGAGAATAAGAGTCTGCGCAGAAGGATCGGGGGGTTCATGATGAGGCAAAACTACAAGCGACATATCCTTCCATGCCATCCAGCCAATTATTTCGATAGAAATTTTGGAAATAGGATGGGTTTCTGCACAAGCTCAAGAGCTGCTGTTTCTGTTTCCAACTATCCATCGGAGGGAACCGATGACCATTTAGACATGACCACCTTGCGTTCTACACCGCGGTACTACCAATTGAATTTGCTACCCAGTGATGTCAGACCTACGCATTCTTCCTTCGCCTTTGCTCCAAGAGGCTTCAAGCCCTGCCAGATCCCTAAATCACAAGAAAAAATTGTATTAGTTTCCCTAGGTAAAGTGACCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

290

Amino Acids

33.24

Weight (kDa)

9.49

Isoelectric Point (pI)

67.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 9 - 43 1.1e-14 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017410)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0326161
rosa_laevigata RLG00000030089
rosa_multiflora Rmu_co8253881.1_g000001 Rmu_sc0000028.1_g000010
rosa_roxburghii Rroxscaffold_4G00324030
rosa_rugosa Rorug01G0057400
rosa_samantha Rh1AG074300 Rh1BG059800 Rh1DG078300 Rh1DG079500
rosa_wichuraiana Rw0G003150 Rw0G020750 Rw1G005960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 527
Acc36I ACCTGC 1 cut(s) 244
AccB1I GGYRCC 1 cut(s) 211
AccII CGCG 2 cut(s) 329, 727
AciI CCGC 3 cut(s) 226, 725, 727
AclWI GGATC 2 cut(s) 542, 811
AcsI RAATTY 2 cut(s) 610, 742
AcyI GRCGYC 1 cut(s) 212
AfaI GTAC 2 cut(s) 170, 731
AfeI AGCGCT 1 cut(s) 270
AfiI CCNNNNNNNGG 2 cut(s) 322, 680
AflII CTTAAG 1 cut(s) 44
AgsI TTSAA 3 cut(s) 158, 742, 805
AluBI AGCT 3 cut(s) 371, 642, 650
AluI AGCT 3 cut(s) 371, 642, 650
Alw21I GWGCWC 2 cut(s) 137, 225
Alw26I GTCTC 2 cut(s) 22, 210
AlwI GGATC 2 cut(s) 542, 811
AlwNI CAGNNNCTG 1 cut(s) 250
Ama87I CYCGRG 2 cut(s) 19, 130
Aor51HI AGCGCT 1 cut(s) 270
AoxI GGCC 3 cut(s) 51, 178, 313
ApeKI GCWGC 2 cut(s) 457, 650
ApoI RAATTY 2 cut(s) 610, 742
AspA2I CCTAGG 1 cut(s) 853
AspLEI GCGC 4 cut(s) 214, 271, 331, 528
AsuC2I CCSGG 2 cut(s) 20, 21
AsuHPI GGTGA 1 cut(s) 96
AvaI CYCGRG 2 cut(s) 19, 130
AvrII CCTAGG 1 cut(s) 853
BanI GGYRCC 1 cut(s) 211
BauI CACGAG 1 cut(s) 509
Bbv12I GWGCWC 2 cut(s) 137, 225
BbvI GCAGC 2 cut(s) 444, 637
BccI CCATC 6 cut(s) 98, 104, 305, 594, 619, 682
BcgI CGANNNNNNTGC 2 cut(s) 517, 551
BcnI CCSGG 2 cut(s) 20, 21
BcoDI GTCTC 2 cut(s) 22, 210
BfaI CTAG 3 cut(s) 420, 483, 854
BfmI CTRYAG 1 cut(s) 251
BfoI RGCGCY 2 cut(s) 215, 272
BfrI CTTAAG 1 cut(s) 44
BfuAI ACCTGC 1 cut(s) 244
BglII AGATCT 2 cut(s) 416, 479
BisI GCNGC 2 cut(s) 458, 651
BlnI CCTAGG 1 cut(s) 853
BlpI GCTNAGC 1 cut(s) 441
BlsI GCNGC 2 cut(s) 459, 652
Bme1390I CCNGG 2 cut(s) 20, 21
BmeT110I CYCGRG 2 cut(s) 19, 130
BmiI GGNNCC 2 cut(s) 213, 685
BmrFI CCNGG 2 cut(s) 20, 21
BmrI ACTGGG 1 cut(s) 747
BmsI GCATC 1 cut(s) 67
BmuI ACTGGG 1 cut(s) 747
Bpu1102I GCTNAGC 1 cut(s) 441
BpuEI CTTGAG 3 cut(s) 419, 482, 628
BpuMI CCSGG 2 cut(s) 20, 21
BsaHI GRCGYC 1 cut(s) 212
BsaJI CCNNGG 4 cut(s) 19, 20, 725, 853
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 680
Bse1I ACTGG 2 cut(s) 181, 753
BseDI CCNNGG 4 cut(s) 19, 20, 725, 853
BseGI GGATG 3 cut(s) 316, 586, 630
BseLI CCNNNNNNNGG 2 cut(s) 322, 680
BseMII CTCAG 2 cut(s) 308, 467
BseNI ACTGG 2 cut(s) 181, 753
BseRI GAGGAG 3 cut(s) 40, 416, 479
BseXI GCAGC 2 cut(s) 444, 637
BsgI GTGCAG 1 cut(s) 619
Bsh1236I CGCG 2 cut(s) 329, 727
BshFI GGCC 3 cut(s) 53, 180, 315
BshNI GGYRCC 1 cut(s) 211
BsiHKAI GWGCWC 2 cut(s) 137, 225
BsiHKCI CYCGRG 2 cut(s) 19, 130
BsiSI CCGG 2 cut(s) 20, 209
BslI CCNNNNNNNGG 2 cut(s) 322, 680
BsmAI GTCTC 2 cut(s) 22, 210
BsmI GAATGC 1 cut(s) 772
BsnI GGCC 3 cut(s) 53, 180, 315
BsoBI CYCGRG 2 cut(s) 19, 130
Bsp1286I GDGCHC 2 cut(s) 137, 225
Bsp143I GATC 4 cut(s) 416, 479, 534, 816
Bsp1720I GCTNAGC 1 cut(s) 441
BspACI CCGC 3 cut(s) 226, 725, 727
BspANI GGCC 3 cut(s) 53, 180, 315
BspCNI CTCAG 2 cut(s) 309, 468
BspFNI CGCG 2 cut(s) 329, 727
BspHI TCATGA 1 cut(s) 546
BspLI GGNNCC 2 cut(s) 213, 685
BspMAI CTGCAG 1 cut(s) 255
BspMI ACCTGC 1 cut(s) 244
BspPI GGATC 2 cut(s) 542, 811
BspQI GCTCTTC 3 cut(s) 213, 426, 489
BspT107I GGYRCC 1 cut(s) 211
BspTI CTTAAG 1 cut(s) 44
BsrI ACTGG 2 cut(s) 181, 753
BssECI CCNNGG 4 cut(s) 19, 20, 725, 853
BssMI GATC 4 cut(s) 416, 479, 534, 816
BssNI GRCGYC 1 cut(s) 212
BssSI CACGAG 1 cut(s) 509
BssT1I CCWWGG 1 cut(s) 853
Bst2BI CACGAG 1 cut(s) 509
Bst6I CTCTTC 3 cut(s) 213, 426, 489
BstACI GRCGYC 1 cut(s) 212
BstAFI CTTAAG 1 cut(s) 44
BstAPI GCANNNNNTGC 2 cut(s) 250, 505
BstC8I GCNNGC 2 cut(s) 333, 438
BstDEI CTNAG 3 cut(s) 317, 441, 476
BstDSI CCRYGG 1 cut(s) 725
BstF5I GGATG 3 cut(s) 316, 586, 630
BstFNI CGCG 2 cut(s) 329, 727
BstH2I RGCGCY 2 cut(s) 215, 272
BstHHI GCGC 4 cut(s) 214, 271, 331, 528
BstKTI GATC 4 cut(s) 419, 482, 537, 819
BstMAI GTCTC 2 cut(s) 22, 210
BstMBI GATC 4 cut(s) 416, 479, 534, 816
BstMWI GCNNNNNNNGC 7 cut(s) 86, 95, 220, 250, 337, 442, 505
BstNSI RCATGY 1 cut(s) 35
BstSCI CCNGG 2 cut(s) 18, 19
BstSFI CTRYAG 1 cut(s) 251
BstUI CGCG 2 cut(s) 329, 727
BstV1I GCAGC 2 cut(s) 444, 637
BstX2I RGATCY 3 cut(s) 416, 479, 816
BstYI RGATCY 3 cut(s) 416, 479, 816
BsuRI GGCC 3 cut(s) 53, 180, 315
BtgI CCRYGG 1 cut(s) 725
BtgZI GCGATG 1 cut(s) 79
BtsCI GGATG 3 cut(s) 316, 586, 630
BtsIMutI CAGTG 1 cut(s) 760
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 2 cut(s) 333, 438
CaiI CAGNNNCTG 1 cut(s) 250
CciI TCATGA 1 cut(s) 546
CfoI GCGC 4 cut(s) 214, 271, 331, 528
Cfr42I CCGCGG 1 cut(s) 728
Cfr9I CCCGGG 1 cut(s) 19
CseI GACGC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 169, 730
CviAII CATG 8 cut(s) 32, 122, 142, 230, 446, 547, 582, 703
CviQI GTAC 2 cut(s) 169, 730
DdeI CTNAG 3 cut(s) 317, 441, 476
DinI GGCGCC 1 cut(s) 213
DpnI GATC 4 cut(s) 418, 481, 536, 818
DpnII GATC 4 cut(s) 416, 479, 534, 816
Eam1104I CTCTTC 3 cut(s) 213, 426, 489
EarI CTCTTC 3 cut(s) 213, 426, 489
Eco130I CCWWGG 1 cut(s) 853
Eco47III AGCGCT 1 cut(s) 270
Eco88I CYCGRG 2 cut(s) 19, 130
EcoT14I CCWWGG 1 cut(s) 853
EgeI GGCGCC 1 cut(s) 213
EheI GGCGCC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 853
FaeI CATG 8 cut(s) 35, 125, 145, 233, 449, 550, 585, 706
FatI CATG 8 cut(s) 31, 121, 141, 229, 445, 546, 581, 702
Fnu4HI GCNGC 2 cut(s) 458, 651
FokI GGATG 3 cut(s) 323, 573, 637
Fsp4HI GCNGC 2 cut(s) 458, 651
FspBI CTAG 3 cut(s) 420, 483, 854
FspI TGCGCA 1 cut(s) 527
GlaI GCGC 4 cut(s) 213, 270, 330, 527
GluI GCNGC 2 cut(s) 458, 651
HaeII RGCGCY 2 cut(s) 215, 272
HaeIII GGCC 3 cut(s) 53, 180, 315
HapII CCGG 2 cut(s) 20, 209
HgaI GACGC 1 cut(s) 335
HhaI GCGC 4 cut(s) 214, 271, 331, 528
Hin1I GRCGYC 1 cut(s) 212
Hin1II CATG 8 cut(s) 35, 125, 145, 233, 449, 550, 585, 706
Hin6I GCGC 4 cut(s) 212, 269, 329, 526
HinP1I GCGC 4 cut(s) 212, 269, 329, 526
HinfI GANTC 3 cut(s) 198, 203, 520
HpaII CCGG 2 cut(s) 20, 209
HphI GGTGA 1 cut(s) 96
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 3 cut(s) 325, 679, 764
Hpy188III TCNNGA 5 cut(s) 155, 426, 489, 547, 645
Hpy8I GTNNAC 1 cut(s) 376
HpyAV CCTTC 4 cut(s) 43, 525, 587, 790
HpyCH4V TGCA 8 cut(s) 14, 80, 239, 253, 289, 412, 508, 636
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 95, 220, 250, 337, 442, 505
HpyF3I CTNAG 3 cut(s) 317, 441, 476
Hsp92I GRCGYC 1 cut(s) 212
Hsp92II CATG 8 cut(s) 35, 125, 145, 233, 449, 550, 585, 706
HspAI GCGC 4 cut(s) 212, 269, 329, 526
KasI GGCGCC 1 cut(s) 211
KspI CCGCGG 1 cut(s) 728
Kzo9I GATC 4 cut(s) 416, 479, 534, 816
LguI GCTCTTC 3 cut(s) 213, 426, 489
LmnI GCTCC 1 cut(s) 796
Lsp1109I GCAGC 2 cut(s) 444, 637
LweI GCATC 1 cut(s) 67
MaeI CTAG 3 cut(s) 420, 483, 854
MaeIII GTNAC 1 cut(s) 862
MalI GATC 4 cut(s) 418, 481, 536, 818
MboI GATC 4 cut(s) 416, 479, 534, 816
MboII GAAGA 4 cut(s) 230, 443, 506, 768
MfeI CAATTG 1 cut(s) 737
MflI RGATCY 3 cut(s) 416, 479, 816
MhlI GDGCHC 2 cut(s) 137, 225
MluCI AATT 6 cut(s) 284, 595, 610, 737, 742, 837
Mly113I GGCGCC 1 cut(s) 212
MlyI GAGTC 3 cut(s) 207, 212, 529
MmeI TCCRAC 2 cut(s) 288, 690
MnlI CCTC 9 cut(s) 18, 76, 191, 197, 394, 457, 546, 673, 791
MseI TTAA 1 cut(s) 45
MslI CAYNNNNRTG 3 cut(s) 140, 228, 450
MspA1I CMGCKG 1 cut(s) 727
MspCI CTTAAG 1 cut(s) 44
MspI CCGG 2 cut(s) 20, 209
MspR9I CCNGG 2 cut(s) 20, 21
MunI CAATTG 1 cut(s) 737
Mva1269I GAATGC 1 cut(s) 772
MvnI CGCG 2 cut(s) 329, 727
MwoI GCNNNNNNNGC 7 cut(s) 86, 95, 220, 250, 337, 442, 505
NarI GGCGCC 1 cut(s) 212
NciI CCSGG 2 cut(s) 20, 21
NdeII GATC 4 cut(s) 416, 479, 534, 816
NlaIII CATG 8 cut(s) 35, 125, 145, 233, 449, 550, 585, 706
NlaIV GGNNCC 2 cut(s) 213, 685
NmuCI GTSAC 1 cut(s) 862
NsbI TGCGCA 1 cut(s) 527
NspI RCATGY 1 cut(s) 35
PaeR7I CTCGAG 1 cut(s) 130
PagI TCATGA 1 cut(s) 546
PciSI GCTCTTC 3 cut(s) 213, 426, 489
PctI GAATGC 1 cut(s) 772
PkrI GCNGC 2 cut(s) 459, 652
PleI GAGTC 3 cut(s) 206, 211, 528
PluTI GGCGCC 1 cut(s) 215
PpsI GAGTC 3 cut(s) 206, 211, 528
PspN4I GGNNCC 2 cut(s) 213, 685
PspXI VCTCGAGB 1 cut(s) 130
PstI CTGCAG 1 cut(s) 255
PstNI CAGNNNCTG 1 cut(s) 250
PsuI RGATCY 3 cut(s) 416, 479, 816
RsaI GTAC 2 cut(s) 170, 731
RsaNI GTAC 2 cut(s) 169, 730
RseI CAYNNNNRTG 3 cut(s) 140, 228, 450
SacII CCGCGG 1 cut(s) 728
SapI GCTCTTC 3 cut(s) 213, 426, 489
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 2 cut(s) 458, 651
Sau3AI GATC 4 cut(s) 416, 479, 534, 816
SchI GAGTC 3 cut(s) 207, 212, 529
ScrFI CCNGG 2 cut(s) 20, 21
SduI GDGCHC 2 cut(s) 137, 225
SfaNI GCATC 1 cut(s) 67
SfcI CTRYAG 1 cut(s) 251
SfoI GGCGCC 1 cut(s) 213
Sfr274I CTCGAG 1 cut(s) 130
Sfr303I CCGCGG 1 cut(s) 728
SgrBI CCGCGG 1 cut(s) 728
SlaI CTCGAG 1 cut(s) 130
SmaI CCCGGG 1 cut(s) 21
SmiMI CAYNNNNRTG 3 cut(s) 140, 228, 450
SmlI CTYRAG 5 cut(s) 44, 130, 398, 461, 643
SmoI CTYRAG 5 cut(s) 44, 130, 398, 461, 643
Sse9I AATT 6 cut(s) 284, 595, 610, 737, 742, 837
SsiI CCGC 3 cut(s) 226, 725, 727
SspDI GGCGCC 1 cut(s) 211
SspMI CTAG 3 cut(s) 420, 483, 854
StyD4I CCNGG 2 cut(s) 18, 19
StyI CCWWGG 1 cut(s) 853
TaqI TCGA 5 cut(s) 131, 201, 425, 488, 603
TasI AATT 6 cut(s) 284, 595, 610, 737, 742, 837
TatI WGTACW 1 cut(s) 168
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 1 cut(s) 760
TseFI GTSAC 1 cut(s) 862
TseI GCWGC 2 cut(s) 457, 650
Tsp45I GTSAC 1 cut(s) 862
TspDTI ATGAA 3 cut(s) 138, 246, 535
TspMI CCCGGG 1 cut(s) 19
TspRI CASTG 1 cut(s) 760
Vha464I CTTAAG 1 cut(s) 44
XapI RAATTY 2 cut(s) 610, 742
XceI RCATGY 1 cut(s) 35
XhoI CTCGAG 1 cut(s) 130
XmaI CCCGGG 1 cut(s) 19
XmaJI CCTAGG 1 cut(s) 853
XspI CTAG 3 cut(s) 420, 483, 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.