Rw1G029730

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0019811
Physical Location & Seq
Forward (+)
57416542 .. 57421598
5057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G029730.1

Sequence Viewer

Length: 1641 bp
ATGGCGACCTCTAATTCCGGTGCTGTTAGCCTGAAACTCCTGATTGATTCAAAGAATCAAAAGGTTCTCTTTGCTGAAGCCGGTAAGGATTTTGTGGATTTTCTTTTCACTCTTCTCTCTTTGCCTGTTGGTACTGTCATTAGGCTTCTCTCCAAGGGTGAGATGGTTGGTAGCTTTGGTAAACCGTATGAGAGGGTTGAAAACCTTGATGATACATATATGCAACCCGAACTAAACAAGGATATCCTGCTTCAACCCAGGGCACTAGTTACTGGACCTAATATCCTCAAGTTGGCCAACAACGTTGAATCAAGTGCTCCAAAGGTGCTCTACTTGTGTCCTGCATTTTATGACGGAACCCAGGCCCACCCGTACGTGACTGAAAATCCCAACGAGGAATGTCCATCGTGTACACGCACCATGGACCTCCAAACTACTTATGTCGGTCAGCCGACTACAACGGCAGGAACTTCTGGTGCCGTCGCTACTGGATATGTGAAAGGCGTTGTGACATACATGATAATGGATGATCTGGAGGTGAAGCCCATGTCCACCATTTCAAGCATCACTATGCTCAACAGGTTGAATATCAAGGATGTTGAGGCTCTTGAAGAGAAGGTTGTTGAACTTGCCATGGATAAGGGTGTGGAGCTATTGAAGGAGTCTTTGTTGTCCAAATCGATCCTCACAAATGTCTTCCTTGGGAAGAAAACTGGGATTGGCATTGATATCTTGGAGCAACTACTACATAAAGGAAATACAACTTTCTCTTCTGGGAGCTGTGCACTCGGTTCTTCTACCCCCTCTTCTGAAGCCAACCCTAGCAGCTCCAGTAATGCTACAAGTACTATATTCATTAGCGGGTGGAAACCTGCTATATCTTCTAAATTTGCTTCCACATTAAACTCTACATCTTTCTCACATGCGTTTCAATTCGATACATCCTCAGCCTCTGCTGCAACCAACACTACATCTACTTCATTTGGAGCATCCTTGATTTCTGCTACTACTTCTAACAGTGCAGCCACAATATTTGGAATGCCCTCCGCTTCTGCTCCTACGTCCAACAGTTTGCCTACTTTGTTTGGAGCATCCACAGTTTCTTCTCCAAGTACCAACAACGCATCGGTGTTTGGATTGTCCAATGGTGCATCATCAACCAGCATGTTCCCCTTTACATCATCTACAACTACTACTACCCCATCACAGCCTGCATTTGGTAACTCCAACTCTACATTTGGCTTCCCTACAGCTCCCTCGGGTAATAATGATCAAATGAATATGGAAGACAGCATGGCTGCACCTCCGCCTACAGTTGCAGTCTTTGCTCAACAGCCTTCCTCAACCACGCAGTTTACACCAACATTCCTAGTGTTTGGCCAGCAGCCTGTGTCAACCCCACAAGCCCCCTTTGTATTTGGAGGTTCCTCCAATGGCTTCCAGTTTCAGAGCCAACCAAACCAATCCAACTCACAGAACCCTCCACCATTTCAGGGTTCCAATAGTTTAGCCAACTTGCAAAACCCTGCACCATTTCAATATAGCCTAGGTTTGACCTCCAATAGCTTCTCATTGGGTGCCGCTGATAAGTCTAATAGAAGGATTGTGAGGGTTAATCACGGCAAGAACAGGAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

546

Amino Acids

57.56

Weight (kDa)

6.15

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 8 - 237 1.2e-47 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019811)

Species Orthologous Gene IDs
pyrus_communis pycom02g21830 pycom02g21890 pycom02g21910
rosa_chinensis RchiOBHm_Chr1g0366041
rosa_multiflora Rmu_sc0003987.1_g000015
rosa_samantha Rh1AG335300
rosa_wichuraiana Rw1G029730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 880
AccB1I GGYRCC 2 cut(s) 476, 1577
AciI CCGC 4 cut(s) 861, 1047, 1307, 1581
AclI AACGTT 1 cut(s) 303
AclWI GGATC 1 cut(s) 676
AcoI YGGCCR 2 cut(s) 294, 1378
AcsI RAATTY 1 cut(s) 887
AcuI CTGAAG 2 cut(s) 96, 831
AfaI GTAC 5 cut(s) 133, 374, 412, 847, 1114
AfiI CCNNNNNNNGG 3 cut(s) 292, 1217, 1493
AhlI ACTAGT 1 cut(s) 265
AjnI CCWGG 2 cut(s) 257, 360
AluBI AGCT 6 cut(s) 174, 652, 780, 828, 1253, 1566
AluI AGCT 6 cut(s) 174, 652, 780, 828, 1253, 1566
Alw21I GWGCWC 3 cut(s) 319, 330, 787
Alw44I GTGCAC 1 cut(s) 783
AlwI GGATC 1 cut(s) 676
AlwNI CAGNNNCTG 1 cut(s) 953
Ama87I CYCGRG 1 cut(s) 1258
AoxI GGCC 3 cut(s) 294, 363, 1378
ApaLI GTGCAC 1 cut(s) 783
ApeKI GCWGC 5 cut(s) 825, 956, 1022, 1298, 1384
ApoI RAATTY 1 cut(s) 887
AspA2I CCTAGG 1 cut(s) 1546
AspS9I GGNCC 3 cut(s) 275, 364, 424
AsuHPI GGTGA 2 cut(s) 170, 550
AvaI CYCGRG 1 cut(s) 1258
AvaII GGWCC 2 cut(s) 275, 424
AvrII CCTAGG 1 cut(s) 1546
BaeGI GKGCMC 2 cut(s) 265, 787
BalI TGGCCA 2 cut(s) 296, 1380
BanI GGYRCC 2 cut(s) 476, 1577
BbsI GAAGAC 2 cut(s) 688, 1293
Bbv12I GWGCWC 3 cut(s) 319, 330, 787
BbvCI CCTCAGC 1 cut(s) 946
BbvI GCAGC 5 cut(s) 837, 943, 1034, 1285, 1396
BccI CCATC 3 cut(s) 157, 412, 1210
BceAI ACGGC 3 cut(s) 464, 477, 1636
BciT130I CCWGG 2 cut(s) 259, 362
BclI TGATCA 1 cut(s) 1270
BcuI ACTAGT 1 cut(s) 265
BfaI CTAG 4 cut(s) 266, 822, 1370, 1547
BfmI CTRYAG 2 cut(s) 1248, 1311
BfuAI ACCTGC 1 cut(s) 880
BisI GCNGC 6 cut(s) 826, 957, 1023, 1299, 1385, 1581
BlnI CCTAGG 1 cut(s) 1546
BlsI GCNGC 6 cut(s) 827, 958, 1024, 1300, 1386, 1582
BmcAI AGTACT 1 cut(s) 847
Bme1390I CCNGG 2 cut(s) 259, 362
Bme18I GGWCC 2 cut(s) 275, 424
BmeT110I CYCGRG 1 cut(s) 1258
BmgT120I GGNCC 3 cut(s) 275, 364, 424
BmiI GGNNCC 5 cut(s) 358, 478, 1426, 1498, 1579
BmrFI CCNGG 2 cut(s) 259, 362
BmrI ACTGGG 1 cut(s) 723
BmsI GCATC 5 cut(s) 573, 998, 1100, 1133, 1160
BmuI ACTGGG 1 cut(s) 723
BpiI GAAGAC 2 cut(s) 688, 1293
BpmI CTGGAG 2 cut(s) 554, 814
Bpu10I CCTNAGC 1 cut(s) 946
BpuEI CTTGAG 1 cut(s) 272
Bsa29I ATCGAT 1 cut(s) 680
BsaAI YACGTR 1 cut(s) 376
BsaJI CCNNGG 9 cut(s) 153, 257, 258, 360, 420, 633, 700, 1257, 1546
BsaWI WCCGGW 1 cut(s) 17
BsaXI ACNNNNNCTCC 2 cut(s) 653, 683
Bsc4I CCNNNNNNNGG 3 cut(s) 292, 1217, 1493
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 5 cut(s) 277, 493, 718, 831, 1441
BseBI CCWGG 2 cut(s) 259, 362
BseCI ATCGAT 1 cut(s) 680
BseDI CCNNGG 9 cut(s) 153, 257, 258, 360, 420, 633, 700, 1257, 1546
BseGI GGATG 5 cut(s) 532, 601, 941, 989, 1091
BseLI CCNNNNNNNGG 3 cut(s) 292, 1217, 1493
BseMII CTCAG 1 cut(s) 960
BseNI ACTGG 5 cut(s) 277, 493, 718, 831, 1441
BseSI GKGCMC 2 cut(s) 265, 787
BseXI GCAGC 5 cut(s) 837, 943, 1034, 1285, 1396
BsgI GTGCAG 3 cut(s) 1041, 1284, 1512
BshFI GGCC 3 cut(s) 296, 365, 1380
BshNI GGYRCC 2 cut(s) 476, 1577
BshVI ATCGAT 1 cut(s) 680
BsiHKAI GWGCWC 3 cut(s) 319, 330, 787
BsiHKCI CYCGRG 1 cut(s) 1258
BsiSI CCGG 2 cut(s) 18, 81
BsiWI CGTACG 1 cut(s) 372
BslI CCNNNNNNNGG 3 cut(s) 292, 1217, 1493
BsmI GAATGC 1 cut(s) 1044
BsnI GGCC 3 cut(s) 296, 365, 1380
BsoBI CYCGRG 1 cut(s) 1258
Bsp1286I GDGCHC 4 cut(s) 265, 319, 330, 787
Bsp1407I TGTACA 1 cut(s) 410
Bsp143I GATC 3 cut(s) 529, 681, 1270
Bsp19I CCATGG 2 cut(s) 420, 633
BspACI CCGC 4 cut(s) 861, 1047, 1307, 1581
BspANI GGCC 3 cut(s) 296, 365, 1380
BspCNI CTCAG 1 cut(s) 959
BspDI ATCGAT 1 cut(s) 680
BspLI GGNNCC 5 cut(s) 358, 478, 1426, 1498, 1579
BspMI ACCTGC 1 cut(s) 880
BspPI GGATC 1 cut(s) 676
BspT107I GGYRCC 2 cut(s) 476, 1577
BsrFI RCCGGY 1 cut(s) 80
BsrGI TGTACA 1 cut(s) 410
BsrI ACTGG 5 cut(s) 277, 493, 718, 831, 1441
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 9 cut(s) 153, 257, 258, 360, 420, 633, 700, 1257, 1546
BssMI GATC 3 cut(s) 529, 681, 1270
BssT1I CCWWGG 5 cut(s) 153, 420, 633, 700, 1546
Bst2UI CCWGG 2 cut(s) 259, 362
Bst4CI ACNGT 6 cut(s) 136, 186, 1019, 1070, 1099, 1315
Bst6I CTCTTC 4 cut(s) 117, 606, 775, 811
BstAPI GCANNNNNTGC 1 cut(s) 1325
BstAUI TGTACA 1 cut(s) 410
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 2 cut(s) 1212, 1382
BstDEI CTNAG 1 cut(s) 946
BstDSI CCRYGG 2 cut(s) 420, 633
BstF5I GGATG 5 cut(s) 532, 601, 941, 989, 1091
BstKTI GATC 3 cut(s) 532, 684, 1273
BstMBI GATC 3 cut(s) 529, 681, 1270
BstMWI GCNNNNNNNGC 2 cut(s) 956, 1325
BstNI CCWGG 2 cut(s) 259, 362
BstNSI RCATGY 2 cut(s) 926, 1168
BstSCI CCNGG 2 cut(s) 257, 360
BstSFI CTRYAG 2 cut(s) 1248, 1311
BstSLI GKGCMC 2 cut(s) 265, 787
BstV1I GCAGC 5 cut(s) 837, 943, 1034, 1285, 1396
BstV2I GAAGAC 2 cut(s) 688, 1293
Bsu15I ATCGAT 1 cut(s) 680
BsuRI GGCC 3 cut(s) 296, 365, 1380
BsuTUI ATCGAT 1 cut(s) 680
BtgI CCRYGG 2 cut(s) 420, 633
BtsCI GGATG 5 cut(s) 532, 601, 941, 989, 1091
BtsIMutI CAGTG 1 cut(s) 1024
BveI ACCTGC 1 cut(s) 880
Cac8I GCNNGC 2 cut(s) 1212, 1382
CaiI CAGNNNCTG 1 cut(s) 953
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 3 cut(s) 275, 364, 424
ClaI ATCGAT 1 cut(s) 680
Csp6I GTAC 5 cut(s) 132, 373, 411, 846, 1113
CspCI CAANNNNNGTGG 2 cut(s) 1015, 1050
CviAII CATG 7 cut(s) 421, 517, 547, 634, 923, 1165, 1294
CviQI GTAC 5 cut(s) 132, 373, 411, 846, 1113
DdeI CTNAG 1 cut(s) 946
DpnI GATC 3 cut(s) 531, 683, 1272
DpnII GATC 3 cut(s) 529, 681, 1270
EaeI YGGCCR 2 cut(s) 294, 1378
Eam1104I CTCTTC 4 cut(s) 117, 606, 775, 811
EarI CTCTTC 4 cut(s) 117, 606, 775, 811
EciI GGCGGA 1 cut(s) 1296
Eco130I CCWWGG 5 cut(s) 153, 420, 633, 700, 1546
Eco32I GATATC 2 cut(s) 244, 730
Eco47I GGWCC 2 cut(s) 275, 424
Eco57I CTGAAG 2 cut(s) 96, 831
Eco88I CYCGRG 1 cut(s) 1258
EcoRII CCWGG 2 cut(s) 257, 360
EcoRV GATATC 2 cut(s) 244, 730
EcoT14I CCWWGG 5 cut(s) 153, 420, 633, 700, 1546
ErhI CCWWGG 5 cut(s) 153, 420, 633, 700, 1546
FaeI CATG 7 cut(s) 424, 520, 550, 637, 926, 1168, 1297
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FatI CATG 7 cut(s) 420, 516, 546, 633, 922, 1164, 1293
FauI CCCGC 1 cut(s) 854
FbaI TGATCA 1 cut(s) 1270
Fnu4HI GCNGC 6 cut(s) 826, 957, 1023, 1299, 1385, 1581
FokI GGATG 5 cut(s) 539, 608, 928, 976, 1078
Fsp4HI GCNGC 6 cut(s) 826, 957, 1023, 1299, 1385, 1581
FspBI CTAG 4 cut(s) 266, 822, 1370, 1547
GluI GCNGC 6 cut(s) 826, 957, 1023, 1299, 1385, 1581
GsuI CTGGAG 2 cut(s) 554, 814
HaeIII GGCC 3 cut(s) 296, 365, 1380
HapII CCGG 2 cut(s) 18, 81
Hin1II CATG 7 cut(s) 424, 520, 550, 637, 926, 1168, 1297
HincII GTYRAC 1 cut(s) 1395
HindII GTYRAC 1 cut(s) 1395
HinfI GANTC 4 cut(s) 47, 55, 308, 662
HpaII CCGG 2 cut(s) 18, 81
HphI GGTGA 2 cut(s) 170, 550
Hpy166II GTNNAC 7 cut(s) 182, 411, 413, 552, 785, 1356, 1395
Hpy188I TCNGA 2 cut(s) 811, 1449
Hpy188III TCNNGA 3 cut(s) 40, 533, 608
Hpy8I GTNNAC 7 cut(s) 182, 411, 413, 552, 785, 1356, 1395
Hpy99I CGWCG 1 cut(s) 485
HpyAV CCTTC 4 cut(s) 610, 652, 1347, 1593
HpyCH4III ACNGT 6 cut(s) 136, 186, 1019, 1070, 1099, 1315
HpyCH4IV ACGT 3 cut(s) 303, 375, 1061
HpyF10VI GCNNNNNNNGC 2 cut(s) 956, 1325
HpyF3I CTNAG 1 cut(s) 946
HpySE526I ACGT 3 cut(s) 303, 375, 1061
Hsp92II CATG 7 cut(s) 424, 520, 550, 637, 926, 1168, 1297
Ksp22I TGATCA 1 cut(s) 1270
Kzo9I GATC 3 cut(s) 529, 681, 1270
LmnI GCTCC 9 cut(s) 322, 649, 736, 777, 833, 986, 1060, 1088, 1258
Lsp1109I GCAGC 5 cut(s) 837, 943, 1034, 1285, 1396
LweI GCATC 5 cut(s) 573, 998, 1100, 1133, 1160
MaeI CTAG 4 cut(s) 266, 822, 1370, 1547
MaeII ACGT 3 cut(s) 303, 375, 1061
MaeIII GTNAC 4 cut(s) 268, 376, 508, 1220
MalI GATC 3 cut(s) 531, 683, 1272
MboI GATC 3 cut(s) 529, 681, 1270
MhlI GDGCHC 4 cut(s) 265, 319, 330, 787
MlsI TGGCCA 2 cut(s) 296, 1380
MluCI AATT 3 cut(s) 13, 887, 932
MluNI TGGCCA 2 cut(s) 296, 1380
MlyI GAGTC 1 cut(s) 671
MmeI TCCRAC 3 cut(s) 1089, 1251, 1491
Mox20I TGGCCA 2 cut(s) 296, 1380
MscI TGGCCA 2 cut(s) 296, 1380
MseI TTAA 2 cut(s) 902, 1614
MslI CAYNNNNRTG 2 cut(s) 521, 569
Msp20I TGGCCA 2 cut(s) 296, 1380
MspA1I CMGCKG 1 cut(s) 1583
MspI CCGG 2 cut(s) 18, 81
MspR9I CCNGG 2 cut(s) 259, 362
Mva1269I GAATGC 1 cut(s) 1044
MvaI CCWGG 2 cut(s) 259, 362
MwoI GCNNNNNNNGC 2 cut(s) 956, 1325
NcoI CCATGG 2 cut(s) 420, 633
NdeII GATC 3 cut(s) 529, 681, 1270
NlaIII CATG 7 cut(s) 424, 520, 550, 637, 926, 1168, 1297
NlaIV GGNNCC 5 cut(s) 358, 478, 1426, 1498, 1579
NmuCI GTSAC 2 cut(s) 376, 508
NspI RCATGY 2 cut(s) 926, 1168
PasI CCCWGGG 1 cut(s) 258
PctI GAATGC 1 cut(s) 1044
PfeI GAWTC 3 cut(s) 47, 55, 308
Pfl23II CGTACG 1 cut(s) 372
PkrI GCNGC 6 cut(s) 827, 958, 1024, 1300, 1386, 1582
PleI GAGTC 1 cut(s) 670
PpsI GAGTC 1 cut(s) 670
Ppu21I YACGTR 1 cut(s) 376
Psp1406I AACGTT 1 cut(s) 303
Psp6I CCWGG 2 cut(s) 257, 360
PspGI CCWGG 2 cut(s) 257, 360
PspLI CGTACG 1 cut(s) 372
PspN4I GGNNCC 5 cut(s) 358, 478, 1426, 1498, 1579
PspPI GGNCC 3 cut(s) 275, 364, 424
PstNI CAGNNNCTG 1 cut(s) 953
RsaI GTAC 5 cut(s) 133, 374, 412, 847, 1114
RsaNI GTAC 5 cut(s) 132, 373, 411, 846, 1113
RseI CAYNNNNRTG 2 cut(s) 521, 569
SaqAI TTAA 2 cut(s) 902, 1614
SatI GCNGC 6 cut(s) 826, 957, 1023, 1299, 1385, 1581
Sau3AI GATC 3 cut(s) 529, 681, 1270
Sau96I GGNCC 3 cut(s) 275, 364, 424
ScaI AGTACT 1 cut(s) 847
SchI GAGTC 1 cut(s) 671
ScrFI CCNGG 2 cut(s) 259, 362
SduI GDGCHC 4 cut(s) 265, 319, 330, 787
SfaNI GCATC 5 cut(s) 573, 998, 1100, 1133, 1160
SfcI CTRYAG 2 cut(s) 1248, 1311
SinI GGWCC 2 cut(s) 275, 424
SmiMI CAYNNNNRTG 2 cut(s) 521, 569
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SpeI ACTAGT 1 cut(s) 265
Sse9I AATT 3 cut(s) 13, 887, 932
SsiI CCGC 4 cut(s) 861, 1047, 1307, 1581
SspI AATATT 1 cut(s) 1032
SspMI CTAG 4 cut(s) 266, 822, 1370, 1547
StyD4I CCNGG 2 cut(s) 257, 360
StyI CCWWGG 5 cut(s) 153, 420, 633, 700, 1546
TaaI ACNGT 6 cut(s) 136, 186, 1019, 1070, 1099, 1315
TaiI ACGT 3 cut(s) 306, 378, 1064
TaqI TCGA 2 cut(s) 680, 936
TaqII GACCGA 1 cut(s) 434
TasI AATT 3 cut(s) 13, 887, 932
TatI WGTACW 2 cut(s) 410, 845
TauI GCSGC 1 cut(s) 1583
TfiI GAWTC 3 cut(s) 47, 55, 308
Tru1I TTAA 2 cut(s) 902, 1614
Tru9I TTAA 2 cut(s) 902, 1614
TscAI CASTG 1 cut(s) 1024
TseFI GTSAC 2 cut(s) 376, 508
TseI GCWGC 5 cut(s) 825, 956, 1022, 1298, 1384
Tsp45I GTSAC 2 cut(s) 376, 508
TspDTI ATGAA 3 cut(s) 844, 969, 1292
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 1 cut(s) 1024
VneI GTGCAC 1 cut(s) 783
VpaK11BI GGWCC 2 cut(s) 275, 424
XapI RAATTY 1 cut(s) 887
XceI RCATGY 2 cut(s) 926, 1168
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XmaJI CCTAGG 1 cut(s) 1546
XspI CTAG 4 cut(s) 266, 822, 1370, 1547
ZrmI AGTACT 1 cut(s) 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.