Rw1G033960

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0018080
Physical Location & Seq
Reverse (-)
61899665 .. 61901205
1541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G033960.1

Sequence Viewer

Length: 1512 bp
ATGGCACTTGCATTTTTGTGGAAAAAGCAACATCTTTGGCCCTTTTCGGTGCTCGAATCTGATGACCTGAGAGTCTCTTATGAGTTAGTCCAAAAGCTCCCGATACCTGAAGACACAAAGAAATTTGTTTACGCAGTTCGTGAGCCCGAAACTCAGTCAGTAATTTATATACTTTGTGTTCAAGATTTCTCAGAAAGGTCAGCATGGGATGCTGAACTTCTGATTAGGGAGATTAAACCTGATGCTGTGATTGTCAAGGAGGATGTTTCGAACCTGAAAGACTTGCAAGTGCAAGAGAGAATGTTGAGTGTTGAAAATCCTGTTCCAGTTTCGAGTTTTGAAGTTATCAAACGATGTTTTAAGGAAGAGTTGAGAGCCGAAACGTATAAGCGCCTTGCGCAAACTTCTGTTTTACAGGAAATCTTTGGGGTTGGCTCTAGTGGACAGTGGTCTGTAGCCAAGAGGGCAGCTCAACATGTTGGCTCATCACTAATGCTGCTTGGAACTCCATTGACTCTGTCCCATTGGAGTGCTACATCAGGTGATTCAGATGGTAAACGTAATGGAATGAGCAGGTTTCAAGACTTGGCTAGTAGTTTGGTTCCGCAAAAAGTGCAATGCGGATGGAGTTGTCTTTCTGATGTGGAATCAAATTTACCAGAAGTTAATCAGCCAGCACAATGCAGTTTTGAGGTCCCCCAATTTGTGCAATCGATTGATCCATTTTTTGTAGCAATTCATGATAAACTTGTGCATGGCTTTCCATCTCTGGGGACTGCTCTGGATCATGTAAGGAAGATATATGATTGTGTCAACAGAGGTGAAGCTGTAGATGCTCTAGTGGTTTCAGAAGTGTACCCTTTTCGGAGAGTGGTGGAGCTGTTGCGATGTATTCATAATGATGAAGGTCGACTCCCAATCAATAGAATGAAAACCATCCTAAGTAGGATTGAGTTTTCTGAGCTTGCATTAGAGGACAAGAAACATGCTCTCTCTGCACAAGCTCTTCGAAGCTTGACTAAAAGATTCAAGACCATAGTAGCAGTTAAATACTGGTACACTCCAATTCCTCCAGAATACAAGGATCTAGCTGCACAGATTGTTACAAGATATGAAGGTGAGGCAGCTAGGCCAGTTCTACAAGGGTCCTCATTCTTGGAACTTGTTCAAGCTTCCATTATCATGAATGTCAAAGGAAAACTGAGAATTCCTACGCCTTCTGATTTTGTGATACGGGCTGCAGAGAACACCAGCTGGTGGGCAATGAGAACAGCATTCTATGAAATGATGAGAGATGGGAAAGTGCATCCCCTTCCTTTGAGAAGATTGGTCGTTAGTATGTCGATTCGTATGAAGTTGTTTTGGCATAAAGTTGGGATCAAATGTGCTGCTGATTCCCTTCCTGCAGCTCCATCAGTTGCTCGTCTGGGTCGCGGTATCAAAAGTTTAAGCCATGCAGCTCAGGTTGTCAAGTATACCCATGAAAGTTTAGGATACCGTCCTCCTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

503

Amino Acids

56.88

Weight (kDa)

9.0

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018080)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g26521 FvH4_7g26521
rosa_chinensis RchiOBHm_Chr1g0373181
rosa_laevigata RLG00000026845
rosa_multiflora Rmu_sc0000170.1_g000014
rosa_rugosa Rorug01G0376600
rosa_samantha Rh1AG385000 Rh1BG348900 Rh1CG362400 Rh1DG379900
rosa_wichuraiana Rw1G033960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
Acc16I TGCGCA 1 cut(s) 399
Acc36I ACCTGC 1 cut(s) 564
AccB7I CCANNNNNTGG 1 cut(s) 1257
AccI GTMKAC 2 cut(s) 910, 1475
AccII CGCG 1 cut(s) 1434
AciI CCGC 3 cut(s) 605, 621, 1434
AclWI GGATC 4 cut(s) 713, 792, 1092, 1385
AcsI RAATTY 3 cut(s) 122, 652, 1206
AcuI CTGAAG 1 cut(s) 129
AfaI GTAC 2 cut(s) 857, 1058
AfiI CCNNNNNNNGG 2 cut(s) 770, 1257
AflIII ACRYGT 1 cut(s) 475
AgsI TTSAA 6 cut(s) 182, 314, 341, 581, 1030, 1169
Alw21I GWGCWC 1 cut(s) 54
Alw26I GTCTC 1 cut(s) 79
AlwI GGATC 4 cut(s) 713, 792, 1092, 1385
AoxI GGCC 2 cut(s) 38, 1130
ApeKI GCWGC 8 cut(s) 467, 496, 1091, 1124, 1238, 1388, 1406, 1457
ApoI RAATTY 3 cut(s) 122, 652, 1206
Asp700I GAANNNNTTC 1 cut(s) 1164
AspA2I CCTAGG 1 cut(s) 1505
AspLEI GCGC 2 cut(s) 393, 400
AspS9I GGNCC 3 cut(s) 39, 694, 1146
AsuHPI GGTGA 3 cut(s) 554, 833, 1130
AsuII TTCGAA 2 cut(s) 269, 1009
AvaII GGWCC 2 cut(s) 694, 1146
AvrII CCTAGG 1 cut(s) 1505
BanII GRGCYC 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 8 cut(s) 479, 483, 1078, 1136, 1225, 1375, 1418, 1469
BccI CCATC 6 cut(s) 545, 618, 772, 944, 1289, 1420
BciVI GTATCC 1 cut(s) 1487
BcoDI GTCTC 1 cut(s) 79
BfaI CTAG 6 cut(s) 438, 591, 839, 1088, 1128, 1506
BfmI CTRYAG 4 cut(s) 453, 828, 1239, 1404
BfoI RGCGCY 1 cut(s) 394
BfuAI ACCTGC 1 cut(s) 564
BfuI GTATCC 1 cut(s) 1487
BglI GCCNNNNNGGC 1 cut(s) 464
BisI GCNGC 8 cut(s) 468, 497, 1092, 1125, 1239, 1389, 1407, 1458
BlnI CCTAGG 1 cut(s) 1505
BlsI GCNGC 8 cut(s) 469, 498, 1093, 1126, 1240, 1390, 1408, 1459
Bme18I GGWCC 2 cut(s) 694, 1146
BmgT120I GGNCC 3 cut(s) 39, 694, 1146
BmiI GGNNCC 3 cut(s) 603, 696, 1147
BmsI GCATC 4 cut(s) 199, 232, 823, 1315
BoxI GACNNNNGTC 1 cut(s) 448
BpiI GAAGAC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 454, 486
BpmI CTGGAG 1 cut(s) 1056
Bpu10I CCTNAGC 1 cut(s) 1461
Bpu14I TTCGAA 2 cut(s) 269, 1009
Bsa29I ATCGAT 1 cut(s) 713
BsaJI CCNNGG 1 cut(s) 1505
Bsc4I CCNNNNNNNGG 2 cut(s) 770, 1257
Bse1I ACTGG 3 cut(s) 326, 1058, 1133
Bse3DI GCAATG 2 cut(s) 623, 1269
BseCI ATCGAT 1 cut(s) 713
BseDI CCNNGG 1 cut(s) 1505
BseGI GGATG 5 cut(s) 214, 268, 629, 936, 1306
BseLI CCNNNNNNNGG 2 cut(s) 770, 1257
BseMI GCAATG 2 cut(s) 623, 1269
BseMII CTCAG 6 cut(s) 59, 167, 204, 951, 1193, 1475
BseNI ACTGG 3 cut(s) 326, 1058, 1133
BseXI GCAGC 8 cut(s) 479, 483, 1078, 1136, 1225, 1375, 1418, 1469
BsgI GTGCAG 2 cut(s) 981, 1077
Bsh1236I CGCG 1 cut(s) 1434
BshFI GGCC 2 cut(s) 40, 1132
BshVI ATCGAT 1 cut(s) 713
BsiHKAI GWGCWC 1 cut(s) 54
BslFI GGGAC 3 cut(s) 505, 680, 787
BslI CCNNNNNNNGG 2 cut(s) 770, 1257
BsmAI GTCTC 1 cut(s) 79
BsmFI GGGAC 3 cut(s) 505, 680, 787
BsmI GAATGC 1 cut(s) 1274
BsnI GGCC 2 cut(s) 40, 1132
Bsp119I TTCGAA 2 cut(s) 269, 1009
Bsp1286I GDGCHC 2 cut(s) 54, 147
Bsp143I GATC 4 cut(s) 718, 784, 1084, 1377
BspACI CCGC 3 cut(s) 605, 621, 1434
BspANI GGCC 2 cut(s) 40, 1132
BspCNI CTCAG 6 cut(s) 60, 166, 203, 952, 1194, 1474
BspDI ATCGAT 1 cut(s) 713
BspFNI CGCG 1 cut(s) 1434
BspHI TCATGA 2 cut(s) 739, 1182
BspLI GGNNCC 3 cut(s) 603, 696, 1147
BspMAI CTGCAG 2 cut(s) 1243, 1408
BspMI ACCTGC 1 cut(s) 564
BspPI GGATC 4 cut(s) 713, 792, 1092, 1385
BspQI GCTCTTC 1 cut(s) 1011
BspT104I TTCGAA 2 cut(s) 269, 1009
BsrDI GCAATG 2 cut(s) 623, 1269
BsrI ACTGG 3 cut(s) 326, 1058, 1133
BssECI CCNNGG 1 cut(s) 1505
BssMI GATC 4 cut(s) 718, 784, 1084, 1377
BssNAI GTATAC 1 cut(s) 1476
BssT1I CCWWGG 1 cut(s) 1505
Bst1107I GTATAC 1 cut(s) 1476
Bst4CI ACNGT 2 cut(s) 447, 1499
Bst6I CTCTTC 2 cut(s) 360, 1011
BstAPI GCANNNNNTGC 2 cut(s) 209, 613
BstBI TTCGAA 2 cut(s) 269, 1009
BstC8I GCNNGC 2 cut(s) 675, 966
BstDEI CTNAG 7 cut(s) 68, 153, 190, 941, 960, 1202, 1461
BstF5I GGATG 5 cut(s) 214, 268, 629, 936, 1306
BstFNI CGCG 1 cut(s) 1434
BstH2I RGCGCY 1 cut(s) 394
BstHHI GCGC 2 cut(s) 393, 400
BstKTI GATC 4 cut(s) 721, 787, 1087, 1380
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 4 cut(s) 718, 784, 1084, 1377
BstMWI GCNNNNNNNGC 6 cut(s) 209, 397, 464, 613, 833, 995
BstNSI RCATGY 2 cut(s) 479, 989
BstPAI GACNNNNGTC 1 cut(s) 448
BstSFI CTRYAG 4 cut(s) 453, 828, 1239, 1404
BstUI CGCG 1 cut(s) 1434
BstV1I GCAGC 8 cut(s) 479, 483, 1078, 1136, 1225, 1375, 1418, 1469
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 1084
BstYI RGATCY 1 cut(s) 1084
BstZ17I GTATAC 1 cut(s) 1476
Bsu15I ATCGAT 1 cut(s) 713
BsuI GTATCC 1 cut(s) 1487
BsuRI GGCC 2 cut(s) 40, 1132
BsuTUI ATCGAT 1 cut(s) 713
BtgZI GCGATG 1 cut(s) 901
BtsCI GGATG 5 cut(s) 214, 268, 629, 936, 1306
BtsIMutI CAGTG 1 cut(s) 452
BveI ACCTGC 1 cut(s) 564
Cac8I GCNNGC 2 cut(s) 675, 966
CciI TCATGA 2 cut(s) 739, 1182
CfoI GCGC 2 cut(s) 393, 400
Cfr13I GGNCC 3 cut(s) 39, 694, 1146
ClaI ATCGAT 1 cut(s) 713
Csp6I GTAC 2 cut(s) 856, 1057
CviAII CATG 9 cut(s) 204, 476, 740, 755, 788, 986, 1183, 1454, 1481
CviQI GTAC 2 cut(s) 856, 1057
DdeI CTNAG 7 cut(s) 68, 153, 190, 941, 960, 1202, 1461
DpnI GATC 4 cut(s) 720, 786, 1086, 1379
DpnII GATC 4 cut(s) 718, 784, 1084, 1377
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
Eam1104I CTCTTC 2 cut(s) 360, 1011
EarI CTCTTC 2 cut(s) 360, 1011
Eco130I CCWWGG 1 cut(s) 1505
Eco24I GRGCYC 1 cut(s) 147
Eco47I GGWCC 2 cut(s) 694, 1146
Eco57I CTGAAG 1 cut(s) 129
EcoO109I RGGNCCY 2 cut(s) 694, 1146
EcoRI GAATTC 1 cut(s) 1206
EcoT14I CCWWGG 1 cut(s) 1505
EcoT38I GRGCYC 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 1505
FaeI CATG 9 cut(s) 207, 479, 743, 758, 791, 989, 1186, 1457, 1484
FaqI GGGAC 3 cut(s) 505, 680, 787
FatI CATG 9 cut(s) 203, 475, 739, 754, 787, 985, 1182, 1453, 1480
FblI GTMKAC 2 cut(s) 910, 1475
Fnu4HI GCNGC 8 cut(s) 468, 497, 1092, 1125, 1239, 1389, 1407, 1458
FokI GGATG 5 cut(s) 221, 275, 636, 923, 1293
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 8 cut(s) 468, 497, 1092, 1125, 1239, 1389, 1407, 1458
FspBI CTAG 6 cut(s) 438, 591, 839, 1088, 1128, 1506
FspI TGCGCA 1 cut(s) 399
GlaI GCGC 2 cut(s) 392, 399
GluI GCNGC 8 cut(s) 468, 497, 1092, 1125, 1239, 1389, 1407, 1458
GsuI CTGGAG 1 cut(s) 1056
HaeII RGCGCY 1 cut(s) 394
HaeIII GGCC 2 cut(s) 40, 1132
HhaI GCGC 2 cut(s) 393, 400
Hin1II CATG 9 cut(s) 207, 479, 743, 758, 791, 989, 1186, 1457, 1484
Hin6I GCGC 2 cut(s) 391, 398
HinP1I GCGC 2 cut(s) 391, 398
HincII GTYRAC 2 cut(s) 814, 911
HindII GTYRAC 2 cut(s) 814, 911
HindIII AAGCTT 2 cut(s) 1012, 1170
HinfI GANTC 9 cut(s) 56, 72, 514, 545, 647, 912, 1026, 1345, 1394
HphI GGTGA 3 cut(s) 554, 833, 1130
Hpy166II GTNNAC 8 cut(s) 130, 443, 557, 814, 856, 911, 1059, 1476
Hpy188I TCNGA 9 cut(s) 61, 193, 222, 550, 640, 850, 867, 961, 1222
Hpy188III TCNNGA 9 cut(s) 100, 140, 182, 581, 740, 782, 1030, 1073, 1183
Hpy8I GTNNAC 8 cut(s) 130, 443, 557, 814, 856, 911, 1059, 1476
HpyAV CCTTC 5 cut(s) 899, 1109, 1227, 1322, 1409
HpyCH4III ACNGT 2 cut(s) 447, 1499
HpyCH4IV ACGT 2 cut(s) 383, 559
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 397, 464, 613, 833, 995
HpyF3I CTNAG 7 cut(s) 68, 153, 190, 941, 960, 1202, 1461
HpySE526I ACGT 2 cut(s) 383, 559
Hsp92II CATG 9 cut(s) 207, 479, 743, 758, 791, 989, 1186, 1457, 1484
HspAI GCGC 2 cut(s) 391, 398
Kzo9I GATC 4 cut(s) 718, 784, 1084, 1377
LguI GCTCTTC 1 cut(s) 1011
LmnI GCTCC 3 cut(s) 102, 877, 1414
Lsp1109I GCAGC 8 cut(s) 479, 483, 1078, 1136, 1225, 1375, 1418, 1469
LweI GCATC 4 cut(s) 199, 232, 823, 1315
MaeI CTAG 6 cut(s) 438, 591, 839, 1088, 1128, 1506
MaeII ACGT 2 cut(s) 383, 559
MaeIII GTNAC 1 cut(s) 1102
MalI GATC 4 cut(s) 720, 786, 1086, 1379
MboI GATC 4 cut(s) 718, 784, 1084, 1377
MboII GAAGA 5 cut(s) 122, 377, 808, 998, 1335
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 2 cut(s) 54, 147
MluCI AATT 7 cut(s) 122, 162, 652, 701, 735, 1065, 1206
MlyI GAGTC 3 cut(s) 81, 508, 906
MnlI CCTC 9 cut(s) 253, 456, 685, 812, 967, 1080, 1114, 1159, 1512
MroXI GAANNNNTTC 1 cut(s) 1164
MseI TTAA 5 cut(s) 234, 360, 666, 1047, 1448
MslI CAYNNNNRTG 4 cut(s) 16, 528, 900, 1181
MspA1I CMGCKG 1 cut(s) 1254
Mva1269I GAATGC 1 cut(s) 1274
MvnI CGCG 1 cut(s) 1434
MwoI GCNNNNNNNGC 6 cut(s) 209, 397, 464, 613, 833, 995
NdeII GATC 4 cut(s) 718, 784, 1084, 1377
NlaIII CATG 9 cut(s) 207, 479, 743, 758, 791, 989, 1186, 1457, 1484
NlaIV GGNNCC 3 cut(s) 603, 696, 1147
NsbI TGCGCA 1 cut(s) 399
NspI RCATGY 2 cut(s) 479, 989
NspV TTCGAA 2 cut(s) 269, 1009
PagI TCATGA 2 cut(s) 739, 1182
PciI ACATGT 1 cut(s) 475
PciSI GCTCTTC 1 cut(s) 1011
PctI GAATGC 1 cut(s) 1274
PdmI GAANNNNTTC 1 cut(s) 1164
PfeI GAWTC 6 cut(s) 56, 545, 647, 1026, 1345, 1394
PflFI GACNNNGTC 1 cut(s) 517
PflMI CCANNNNNTGG 1 cut(s) 1257
PkrI GCNGC 8 cut(s) 469, 498, 1093, 1126, 1240, 1390, 1408, 1459
PleI GAGTC 3 cut(s) 80, 508, 906
PpsI GAGTC 3 cut(s) 80, 508, 906
PpuMI RGGWCCY 2 cut(s) 694, 1146
PscI ACATGT 1 cut(s) 475
PshAI GACNNNNGTC 1 cut(s) 448
Psp5II RGGWCCY 2 cut(s) 694, 1146
PspN4I GGNNCC 3 cut(s) 603, 696, 1147
PspPI GGNCC 3 cut(s) 39, 694, 1146
PspPPI RGGWCCY 2 cut(s) 694, 1146
PstI CTGCAG 2 cut(s) 1243, 1408
PsuI RGATCY 1 cut(s) 1084
PsyI GACNNNGTC 1 cut(s) 517
PvuII CAGCTG 1 cut(s) 1254
RsaI GTAC 2 cut(s) 857, 1058
RsaNI GTAC 2 cut(s) 856, 1057
RseI CAYNNNNRTG 4 cut(s) 16, 528, 900, 1181
SalI GTCGAC 1 cut(s) 909
SapI GCTCTTC 1 cut(s) 1011
SaqAI TTAA 5 cut(s) 234, 360, 666, 1047, 1448
SatI GCNGC 8 cut(s) 468, 497, 1092, 1125, 1239, 1389, 1407, 1458
Sau3AI GATC 4 cut(s) 718, 784, 1084, 1377
Sau96I GGNCC 3 cut(s) 39, 694, 1146
SchI GAGTC 3 cut(s) 81, 508, 906
SduI GDGCHC 2 cut(s) 54, 147
SfaNI GCATC 4 cut(s) 199, 232, 823, 1315
SfcI CTRYAG 4 cut(s) 453, 828, 1239, 1404
SfuI TTCGAA 2 cut(s) 269, 1009
SinI GGWCC 2 cut(s) 694, 1146
SmiMI CAYNNNNRTG 4 cut(s) 16, 528, 900, 1181
Sse9I AATT 7 cut(s) 122, 162, 652, 701, 735, 1065, 1206
SsiI CCGC 3 cut(s) 605, 621, 1434
SspMI CTAG 6 cut(s) 438, 591, 839, 1088, 1128, 1506
StyI CCWWGG 1 cut(s) 1505
TaaI ACNGT 2 cut(s) 447, 1499
TaiI ACGT 2 cut(s) 386, 562
TaqI TCGA 7 cut(s) 54, 269, 332, 713, 910, 1009, 1343
TasI AATT 7 cut(s) 122, 162, 652, 701, 735, 1065, 1206
TfiI GAWTC 6 cut(s) 56, 545, 647, 1026, 1345, 1394
Tru1I TTAA 5 cut(s) 234, 360, 666, 1047, 1448
Tru9I TTAA 5 cut(s) 234, 360, 666, 1047, 1448
TscAI CASTG 1 cut(s) 452
TseI GCWGC 8 cut(s) 467, 496, 1091, 1124, 1238, 1388, 1406, 1457
TspDTI ATGAA 9 cut(s) 728, 884, 918, 944, 1128, 1199, 1296, 1367, 1497
TspRI CASTG 1 cut(s) 452
Tth111I GACNNNGTC 1 cut(s) 517
Van91I CCANNNNNTGG 1 cut(s) 1257
VpaK11BI GGWCC 2 cut(s) 694, 1146
XapI RAATTY 3 cut(s) 122, 652, 1206
XceI RCATGY 2 cut(s) 479, 989
XmaJI CCTAGG 1 cut(s) 1505
XmiI GTMKAC 2 cut(s) 910, 1475
XmnI GAANNNNTTC 1 cut(s) 1164
XspI CTAG 6 cut(s) 438, 591, 839, 1088, 1128, 1506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.