Rw2G054670

Protein of unknown function (DUF3339)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0019561
Physical Location & Seq
Forward (+)
82244945 .. 82247680
2736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G054670.1

Sequence Viewer

Length: 447 bp
ATGTCGGATTGGGGGCCAGTTTTCATAGCAGTGGTGCTGTTCATACTGTTAACTCCAGGGCTGCTGATTCAGATACCTGGGAAAAGCCGGTTCGTCGAGTTTGGCAATTTTCAGACTAGTGGAGTATCAATTCTGGTTCATTCCATCATCTACTTTACTCTCATCTGTATCTTCTTGTTGGCTATTGGATTTTGCCGGACTTGGGGTTTTGATTTGAAGCTCTTAACTTTGAAGGAAATGGCGGACTGGGGCCCAGTACTGATTGGGGTTGTGCTGTTTATTCTGCTCCAACCGGGACTCCTCTTCTCGTTGCCGGGAAACGGCAAGCAGGTCGAGTTTGGGAGCATGAAGACCAACGGCAAAGCCATAGCTGTGCACACACTCATTTTTTTTGCGCTCTATGCCGTTCTCGTCTTGGCCGTACACGTCCACATCTACACCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3339

Protein Analysis

148

Amino Acids

16.27

Weight (kDa)

8.66

Isoelectric Point (pI)

18.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3339 PF11820 1 - 63 1.5e-32 Protein of unknown function (DUF3339)
DUF3339 PF11820 80 - 146 1.1e-31 Protein of unknown function (DUF3339)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019561)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27030
prunus_persica Prupe.3G307400_v2.0.a1
pyrus_communis pycom111g00770
rosa_chinensis RchiOBHm_Chr2g0175791
rosa_roxburghii Rroxscaffold_2G00077020
rosa_rugosa Rorug02G0587700
rosa_wichuraiana Rw2G054670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 319
AciI CCGC 1 cut(s) 242
AcoI YGGCCR 1 cut(s) 417
AfaI GTAC 2 cut(s) 258, 423
AfiI CCNNNNNNNGG 2 cut(s) 202, 320
AflIII ACRYGT 1 cut(s) 424
AgeI ACCGGT 1 cut(s) 440
AgsI TTSAA 2 cut(s) 217, 232
AhlI ACTAGT 1 cut(s) 116
AjiI CACGTC 1 cut(s) 427
AjnI CCWGG 2 cut(s) 55, 76
AluBI AGCT 2 cut(s) 220, 371
AluI AGCT 2 cut(s) 220, 371
Alw21I GWGCWC 1 cut(s) 378
Alw44I GTGCAC 1 cut(s) 374
AoxI GGCC 3 cut(s) 14, 250, 417
ApaI GGGCCC 1 cut(s) 254
ApaLI GTGCAC 1 cut(s) 374
ApeKI GCWGC 1 cut(s) 61
AsiGI ACCGGT 1 cut(s) 440
AspLEI GCGC 1 cut(s) 397
AspS9I GGNCC 3 cut(s) 14, 250, 251
AsuC2I CCSGG 2 cut(s) 294, 315
BaeGI GKGCMC 2 cut(s) 254, 378
BanII GRGCYC 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 356
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 1 cut(s) 48
BccI CCATC 1 cut(s) 152
BceAI ACGGC 4 cut(s) 337, 373, 389, 404
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 2 cut(s) 57, 78
BcnI CCSGG 2 cut(s) 294, 315
BcuI ACTAGT 1 cut(s) 116
BfaI CTAG 1 cut(s) 117
BfuAI ACCTGC 1 cut(s) 319
BisI GCNGC 1 cut(s) 62
BlsI GCNGC 1 cut(s) 63
BmcAI AGTACT 1 cut(s) 258
Bme1390I CCNGG 4 cut(s) 57, 78, 294, 315
BmgBI CACGTC 1 cut(s) 427
BmgT120I GGNCC 3 cut(s) 14, 250, 251
BmiI GGNNCC 3 cut(s) 15, 251, 252
BmrFI CCNGG 4 cut(s) 57, 78, 294, 315
BmrI ACTGGG 2 cut(s) 248, 256
BmuI ACTGGG 2 cut(s) 248, 256
BpiI GAAGAC 1 cut(s) 356
BpmI CTGGAG 1 cut(s) 39
BpuMI CCSGG 2 cut(s) 294, 315
BsaJI CCNNGG 2 cut(s) 56, 77
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 2 cut(s) 282, 312
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 320
Bse118I RCCGGY 2 cut(s) 87, 440
Bse1I ACTGG 3 cut(s) 17, 251, 254
BseBI CCWGG 2 cut(s) 57, 78
BseDI CCNNGG 2 cut(s) 56, 77
BseLI CCNNNNNNNGG 2 cut(s) 202, 320
BseNI ACTGG 3 cut(s) 17, 251, 254
BseRI GAGGAG 1 cut(s) 290
BseSI GKGCMC 2 cut(s) 254, 378
BseXI GCAGC 1 cut(s) 48
BshFI GGCC 3 cut(s) 16, 252, 419
BshTI ACCGGT 1 cut(s) 440
BsiHKAI GWGCWC 1 cut(s) 378
BsiSI CCGG 5 cut(s) 88, 196, 293, 314, 441
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 2 cut(s) 202, 320
BsmFI GGGAC 1 cut(s) 309
BsnI GGCC 3 cut(s) 16, 252, 419
Bsp120I GGGCCC 1 cut(s) 250
Bsp1286I GDGCHC 2 cut(s) 254, 378
BspACI CCGC 1 cut(s) 242
BspANI GGCC 3 cut(s) 16, 252, 419
BspLI GGNNCC 3 cut(s) 15, 251, 252
BspMI ACCTGC 1 cut(s) 319
BsrFI RCCGGY 2 cut(s) 87, 440
BsrI ACTGG 3 cut(s) 17, 251, 254
BssAI RCCGGY 2 cut(s) 87, 440
BssECI CCNNGG 2 cut(s) 56, 77
Bst2UI CCWGG 2 cut(s) 57, 78
Bst4CI ACNGT 1 cut(s) 48
Bst6I CTCTTC 1 cut(s) 308
BstC8I GCNNGC 1 cut(s) 326
BstHHI GCGC 1 cut(s) 397
BstMWI GCNNNNNNNGC 1 cut(s) 401
BstNI CCWGG 2 cut(s) 57, 78
BstSCI CCNGG 4 cut(s) 55, 76, 292, 313
BstSLI GKGCMC 2 cut(s) 254, 378
BstV1I GCAGC 1 cut(s) 48
BstV2I GAAGAC 1 cut(s) 356
BsuRI GGCC 3 cut(s) 16, 252, 419
BtrI CACGTC 1 cut(s) 427
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 1 cut(s) 36
BveI ACCTGC 1 cut(s) 319
Cac8I GCNNGC 1 cut(s) 326
CfoI GCGC 1 cut(s) 397
Cfr10I RCCGGY 2 cut(s) 87, 440
Cfr13I GGNCC 3 cut(s) 14, 250, 251
Csp6I GTAC 2 cut(s) 257, 422
CspAI ACCGGT 1 cut(s) 440
CviAII CATG 1 cut(s) 346
CviJI RGCY 9 cut(s) 16, 61, 87, 182, 220, 252, 365, 371, 419
CviKI_1 RGCY 9 cut(s) 16, 61, 87, 182, 220, 252, 365, 371, 419
CviQI GTAC 2 cut(s) 257, 422
EaeI YGGCCR 1 cut(s) 417
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
EciI GGCGGA 1 cut(s) 257
Eco24I GRGCYC 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 250
EcoRII CCWGG 2 cut(s) 55, 76
EcoT38I GRGCYC 1 cut(s) 254
FaeI CATG 1 cut(s) 349
FaiI YATR 5 cut(s) 26, 44, 347, 368, 402
FaqI GGGAC 1 cut(s) 309
FatI CATG 1 cut(s) 345
Fnu4HI GCNGC 1 cut(s) 62
FriOI GRGCYC 1 cut(s) 254
Fsp4HI GCNGC 1 cut(s) 62
FspBI CTAG 1 cut(s) 117
GlaI GCGC 1 cut(s) 396
GluI GCNGC 1 cut(s) 62
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 3 cut(s) 16, 252, 419
HapII CCGG 5 cut(s) 88, 196, 293, 314, 441
HhaI GCGC 1 cut(s) 397
Hin1II CATG 1 cut(s) 349
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HinfI GANTC 2 cut(s) 67, 297
HpaI GTTAAC 1 cut(s) 51
HpaII CCGG 5 cut(s) 88, 196, 293, 314, 441
Hpy166II GTNNAC 4 cut(s) 51, 376, 424, 430
Hpy188I TCNGA 3 cut(s) 7, 72, 114
Hpy8I GTNNAC 4 cut(s) 51, 376, 424, 430
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 1 cut(s) 48
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 1 cut(s) 376
HpyF10VI GCNNNNNNNGC 1 cut(s) 401
HpySE526I ACGT 1 cut(s) 426
Hsp92II CATG 1 cut(s) 349
HspAI GCGC 1 cut(s) 395
KspAI GTTAAC 1 cut(s) 51
LmnI GCTCC 2 cut(s) 291, 342
Lsp1109I GCAGC 1 cut(s) 48
MaeI CTAG 1 cut(s) 117
MaeII ACGT 1 cut(s) 426
MboII GAAGA 3 cut(s) 163, 295, 361
MhlI GDGCHC 2 cut(s) 254, 378
MluCI AATT 2 cut(s) 106, 129
MlyI GAGTC 1 cut(s) 291
MmeI TCCRAC 1 cut(s) 313
MnlI CCTC 1 cut(s) 311
MseI TTAA 2 cut(s) 50, 224
MslI CAYNNNNRTG 2 cut(s) 29, 371
MspI CCGG 5 cut(s) 88, 196, 293, 314, 441
MspR9I CCNGG 4 cut(s) 57, 78, 294, 315
MvaI CCWGG 2 cut(s) 57, 78
MwoI GCNNNNNNNGC 1 cut(s) 401
NciI CCSGG 2 cut(s) 294, 315
NlaIII CATG 1 cut(s) 349
NlaIV GGNNCC 3 cut(s) 15, 251, 252
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PfeI GAWTC 1 cut(s) 67
PinAI ACCGGT 1 cut(s) 440
PkrI GCNGC 1 cut(s) 63
PleI GAGTC 1 cut(s) 291
PpsI GAGTC 1 cut(s) 291
Psp6I CCWGG 2 cut(s) 55, 76
PspGI CCWGG 2 cut(s) 55, 76
PspN4I GGNNCC 3 cut(s) 15, 251, 252
PspOMI GGGCCC 1 cut(s) 250
PspPI GGNCC 3 cut(s) 14, 250, 251
RsaI GTAC 2 cut(s) 258, 423
RsaNI GTAC 2 cut(s) 257, 422
RseI CAYNNNNRTG 2 cut(s) 29, 371
SaqAI TTAA 2 cut(s) 50, 224
SatI GCNGC 1 cut(s) 62
Sau96I GGNCC 3 cut(s) 14, 250, 251
ScaI AGTACT 1 cut(s) 258
SchI GAGTC 1 cut(s) 291
ScrFI CCNGG 4 cut(s) 57, 78, 294, 315
SduI GDGCHC 2 cut(s) 254, 378
SetI ASST 5 cut(s) 79, 222, 333, 373, 429
SmiMI CAYNNNNRTG 2 cut(s) 29, 371
SpeI ACTAGT 1 cut(s) 116
Sse9I AATT 2 cut(s) 106, 129
SsiI CCGC 1 cut(s) 242
SspMI CTAG 1 cut(s) 117
StyD4I CCNGG 4 cut(s) 55, 76, 292, 313
TaaI ACNGT 1 cut(s) 48
TaiI ACGT 1 cut(s) 429
TaqI TCGA 2 cut(s) 96, 333
TasI AATT 2 cut(s) 106, 129
TatI WGTACW 1 cut(s) 256
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 2 cut(s) 50, 224
Tru9I TTAA 2 cut(s) 50, 224
TscAI CASTG 1 cut(s) 36
TseI GCWGC 1 cut(s) 61
TspDTI ATGAA 4 cut(s) 13, 31, 128, 362
TspRI CASTG 1 cut(s) 36
VneI GTGCAC 1 cut(s) 374
XspI CTAG 1 cut(s) 117
ZrmI AGTACT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.