Rw3G010430

BTB POZ domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0009361
Physical Location & Seq
Forward (+)
10069617 .. 10073683
4067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G010430.1

Sequence Viewer

Length: 1110 bp
ATGTTGCCGCCTCCGTTGTCAGGAATCGACGCCTCGATCGTCACAACTGTACAGCCCGCCTCCGACGGCCTCCCCTCGGCGATCACAGCCGGTACCGACGGCTCCGTTTGGATCGCACACGGCGGTCAAATATCCAGCTACGATTGGAATCTGTGCCACGCCGGAACGATCCGTACTCATTTGGAGGACATAAACTCGATTTGCCGAGTGTATCCTGAAATCGCCGCCGTAGGATCCGAATCCGACCCGGGACTCCACTTCTACGACTTCTCCGGCGTGCGAAACATGGGGTCGATCCACTGGACCGACCCGGCCGACCCGAGAATCTTCAAGGCCCGAGTTACCTCCATCTCTAACTCGCCGAGCTCAGTCTTCGCCGCTTTCGATTGCCCGCACAGAGAGAACTGCATCTTAGTGATCGATAAATCAACGCTCAAGATCGTCGGTGAGCTGAGCAGGCAATCCGGAAGCTCGGCGAAGAACTTGGCCGTCGGAAAGCTGACGTGGCTGCCTGAAACGGGCTTGATTGTTGGGAGCTCCGTCACTTGCGGTGCGTTCGGGTACTCGGGTTATATCCGAATGTGGGACCCGAGGTCCAAGGAGGTGGTTTGGGAGACGAACGAGCCAGGGTCGGGGCGGAGCAGTAGGTTCGGGGACTCCTTTGCGGACGTCGACGTGGACATTGAGGAGTCGACTCTGTTCAAGGTGTGTTCAAAGTCTGGAGATTTGGCAATAGCAGACCTGCGTAATTTAGGGGATGATCCGTGGGTTTATTTACAAGATAAGGACCCAAGCATGAGGAACATTAAGGGGGGTGGCAATAGCAGTAGTGTAATTCACTGTTACAAAAACCAAGCCTTTGTGGGCAGAGATGGTGCCTTGGAGGTTTGGTCTAGACTGCCGAGAGCCGAAAATAGGGTGGATAGAGAAAGTGAGGTTTGCGAGGGATTGTATAGGAGGAATTTTGTGGATAAAGAGGAGGATGCTCAGAGAGGGATTATAAAGAAAATCGAAGGCGGTGGGAATAGACTGTTTGTTGTTAGAGAAGATGTTGAAGGTATTGAGGTGTGGGAGAGCTCTCGTTCTTCTGGTGCAGTTTCAGTTTTGTGA

Protein Analysis

369

Amino Acids

40.15

Weight (kDa)

5.14

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta_prop_At2g24240 PF25279 25 - 358 6.1e-19 At2g24240-like, C-terminal beta propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1001
AatII GACGTC 1 cut(s) 672
Acc36I ACCTGC 1 cut(s) 750
Acc65I GGTACC 1 cut(s) 92
AccB1I GGYRCC 2 cut(s) 92, 875
AccI GTMKAC 2 cut(s) 672, 692
AccIII TCCGGA 1 cut(s) 464
AclWI GGATC 6 cut(s) 119, 163, 228, 241, 289, 755
AcoI YGGCCR 2 cut(s) 312, 486
AcsI RAATTY 1 cut(s) 961
AcyI GRCGYC 2 cut(s) 30, 669
AfaI GTAC 4 cut(s) 51, 94, 175, 563
AfiI CCNNNNNNNGG 6 cut(s) 20, 76, 518, 583, 632, 915
AgsI TTSAA 4 cut(s) 331, 703, 714, 1055
AhdI GACNNNNNGTC 1 cut(s) 592
AjiI CACGTC 2 cut(s) 504, 676
AjnI CCWGG 1 cut(s) 625
AluBI AGCT 7 cut(s) 138, 366, 451, 471, 499, 537, 1077
AluI AGCT 7 cut(s) 138, 366, 451, 471, 499, 537, 1077
Alw21I GWGCWC 3 cut(s) 368, 539, 1079
Alw26I GTCTC 1 cut(s) 608
AlwI GGATC 6 cut(s) 119, 163, 228, 241, 289, 755
Ama87I CYCGRG 5 cut(s) 247, 319, 336, 565, 589
Aor13HI TCCGGA 1 cut(s) 464
AoxI GGCC 4 cut(s) 67, 312, 333, 486
ApeKI GCWGC 1 cut(s) 508
ApoI RAATTY 1 cut(s) 961
Asp718I GGTACC 1 cut(s) 92
AspS9I GGNCC 5 cut(s) 303, 334, 586, 594, 787
AsuC2I CCSGG 3 cut(s) 248, 249, 311
AsuHPI GGTGA 1 cut(s) 458
AvaI CYCGRG 5 cut(s) 247, 319, 336, 565, 589
AvaII GGWCC 4 cut(s) 303, 586, 594, 787
BaeI ACNNNNGTAYC 2 cut(s) 553, 586
BamHI GGATCC 1 cut(s) 233
BanI GGYRCC 2 cut(s) 92, 875
BanII GRGCYC 3 cut(s) 368, 539, 1079
BbsI GAAGAC 1 cut(s) 364
Bbv12I GWGCWC 3 cut(s) 368, 539, 1079
BbvI GCAGC 1 cut(s) 495
BccI CCATC 2 cut(s) 356, 866
BceAI ACGGC 5 cut(s) 82, 115, 136, 212, 473
BcgI CGANNNNNNTGC 2 cut(s) 631, 665
BciT130I CCWGG 1 cut(s) 627
BciVI GTATCC 1 cut(s) 222
BcnI CCSGG 3 cut(s) 248, 249, 311
BcoDI GTCTC 1 cut(s) 608
BfaI CTAG 1 cut(s) 894
BfuAI ACCTGC 1 cut(s) 750
BfuI GTATCC 1 cut(s) 222
BisI GCNGC 4 cut(s) 8, 225, 378, 509
BlpI GCTNAGC 1 cut(s) 452
BlsI GCNGC 4 cut(s) 9, 226, 379, 510
Bme1390I CCNGG 4 cut(s) 248, 249, 311, 627
Bme18I GGWCC 4 cut(s) 303, 586, 594, 787
BmeRI GACNNNNNGTC 1 cut(s) 592
BmeT110I CYCGRG 5 cut(s) 247, 319, 336, 565, 589
BmgBI CACGTC 2 cut(s) 504, 676
BmgT120I GGNCC 5 cut(s) 303, 334, 586, 594, 787
BmiI GGNNCC 7 cut(s) 94, 103, 235, 587, 588, 789, 877
BmrFI CCNGG 4 cut(s) 248, 249, 311, 627
BmsI GCATC 2 cut(s) 417, 973
BpiI GAAGAC 1 cut(s) 364
BpmI CTGGAG 1 cut(s) 741
Bpu1102I GCTNAGC 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 419
BpuMI CCSGG 3 cut(s) 248, 249, 311
Bsa29I ATCGAT 1 cut(s) 420
BsaBI GATNNNNATC 2 cut(s) 147, 238
BsaHI GRCGYC 2 cut(s) 30, 669
BsaJI CCNNGG 7 cut(s) 75, 247, 590, 597, 626, 764, 879
BsaWI WCCGGW 1 cut(s) 464
BsaXI ACNNNNNCTCC 4 cut(s) 254, 284, 875, 905
Bsc4I CCNNNNNNNGG 6 cut(s) 20, 76, 518, 583, 632, 915
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 1 cut(s) 305
Bse8I GATNNNNATC 2 cut(s) 147, 238
BseAI TCCGGA 1 cut(s) 464
BseBI CCWGG 1 cut(s) 627
BseCI ATCGAT 1 cut(s) 420
BseDI CCNNGG 7 cut(s) 75, 247, 590, 597, 626, 764, 879
BseGI GGATG 2 cut(s) 763, 988
BseJI GATNNNNATC 2 cut(s) 147, 238
BseLI CCNNNNNNNGG 6 cut(s) 20, 76, 518, 583, 632, 915
BseMII CTCAG 3 cut(s) 381, 443, 1001
BseNI ACTGG 1 cut(s) 305
BseRI GAGGAG 2 cut(s) 701, 992
BseX3I CGGCCG 1 cut(s) 312
BseXI GCAGC 1 cut(s) 495
Bsh1285I CGRYCG 2 cut(s) 39, 315
BshFI GGCC 4 cut(s) 69, 314, 335, 488
BshNI GGYRCC 2 cut(s) 92, 875
BshVI ATCGAT 1 cut(s) 420
BsiEI CGRYCG 2 cut(s) 39, 315
BsiHKAI GWGCWC 3 cut(s) 368, 539, 1079
BsiHKCI CYCGRG 5 cut(s) 247, 319, 336, 565, 589
BsiSI CCGG 6 cut(s) 90, 162, 248, 273, 311, 465
BslFI GGGAC 3 cut(s) 264, 599, 668
BslI CCNNNNNNNGG 6 cut(s) 20, 76, 518, 583, 632, 915
BsmAI GTCTC 1 cut(s) 608
BsmBI CGTCTC 1 cut(s) 608
BsmFI GGGAC 3 cut(s) 264, 599, 668
BsnI GGCC 4 cut(s) 69, 314, 335, 488
BsoBI CYCGRG 5 cut(s) 247, 319, 336, 565, 589
Bsp1286I GDGCHC 3 cut(s) 368, 539, 1079
Bsp13I TCCGGA 1 cut(s) 464
Bsp1407I TGTACA 1 cut(s) 49
Bsp143I GATC 9 cut(s) 36, 81, 111, 168, 233, 294, 417, 438, 760
Bsp1720I GCTNAGC 1 cut(s) 452
BspANI GGCC 4 cut(s) 69, 314, 335, 488
BspCNI CTCAG 3 cut(s) 380, 444, 1000
BspDI ATCGAT 1 cut(s) 420
BspEI TCCGGA 1 cut(s) 464
BspLI GGNNCC 7 cut(s) 94, 103, 235, 587, 588, 789, 877
BspMI ACCTGC 1 cut(s) 750
BspPI GGATC 6 cut(s) 119, 163, 228, 241, 289, 755
BspT107I GGYRCC 2 cut(s) 92, 875
BsrFI RCCGGY 1 cut(s) 89
BsrGI TGTACA 1 cut(s) 49
BsrI ACTGG 1 cut(s) 305
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 7 cut(s) 75, 247, 590, 597, 626, 764, 879
BssMI GATC 9 cut(s) 36, 81, 111, 168, 233, 294, 417, 438, 760
BssNI GRCGYC 2 cut(s) 30, 669
BssT1I CCWWGG 2 cut(s) 597, 879
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 3 cut(s) 49, 842, 1032
BstACI GRCGYC 2 cut(s) 30, 669
BstAUI TGTACA 1 cut(s) 49
BstC8I GCNNGC 4 cut(s) 57, 278, 392, 458
BstDEI CTNAG 4 cut(s) 367, 412, 452, 987
BstDSI CCRYGG 1 cut(s) 764
BstF5I GGATG 2 cut(s) 763, 988
BstKTI GATC 9 cut(s) 39, 84, 114, 171, 236, 297, 420, 441, 763
BstMAI GTCTC 1 cut(s) 608
BstMBI GATC 9 cut(s) 36, 81, 111, 168, 233, 294, 417, 438, 760
BstMCI CGRYCG 2 cut(s) 39, 315
BstMWI GCNNNNNNNGC 3 cut(s) 86, 457, 505
BstNI CCWGG 1 cut(s) 627
BstSCI CCNGG 4 cut(s) 246, 247, 309, 625
BstV1I GCAGC 1 cut(s) 495
BstV2I GAAGAC 1 cut(s) 364
BstX2I RGATCY 1 cut(s) 233
BstXI CCANNNNNNTGG 1 cut(s) 604
BstYI RGATCY 1 cut(s) 233
BstZI CGGCCG 1 cut(s) 312
Bsu15I ATCGAT 1 cut(s) 420
BsuI GTATCC 1 cut(s) 222
BsuRI GGCC 4 cut(s) 69, 314, 335, 488
BsuTUI ATCGAT 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 764
BtrI CACGTC 2 cut(s) 504, 676
BtsCI GGATG 2 cut(s) 763, 988
BtsIMutI CAGTG 2 cut(s) 298, 838
BveI ACCTGC 1 cut(s) 750
Cac8I GCNNGC 4 cut(s) 57, 278, 392, 458
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 5 cut(s) 303, 334, 586, 594, 787
Cfr9I CCCGGG 1 cut(s) 247
ClaI ATCGAT 1 cut(s) 420
CseI GACGC 1 cut(s) 38
Csp6I GTAC 4 cut(s) 50, 93, 174, 562
CviAII CATG 2 cut(s) 286, 796
CviQI GTAC 4 cut(s) 50, 93, 174, 562
DdeI CTNAG 4 cut(s) 367, 412, 452, 987
DpnI GATC 9 cut(s) 38, 83, 113, 170, 235, 296, 419, 440, 762
DpnII GATC 9 cut(s) 36, 81, 111, 168, 233, 294, 417, 438, 760
DriI GACNNNNNGTC 1 cut(s) 592
EaeI YGGCCR 2 cut(s) 312, 486
EagI CGGCCG 1 cut(s) 312
Eam1105I GACNNNNNGTC 1 cut(s) 592
EciI GGCGGA 1 cut(s) 652
Ecl136II GAGCTC 3 cut(s) 366, 537, 1077
EclXI CGGCCG 1 cut(s) 312
Eco130I CCWWGG 2 cut(s) 597, 879
Eco24I GRGCYC 3 cut(s) 368, 539, 1079
Eco47I GGWCC 4 cut(s) 303, 586, 594, 787
Eco52I CGGCCG 1 cut(s) 312
Eco53kI GAGCTC 3 cut(s) 366, 537, 1077
Eco88I CYCGRG 5 cut(s) 247, 319, 336, 565, 589
EcoICRI GAGCTC 3 cut(s) 366, 537, 1077
EcoO109I RGGNCCY 2 cut(s) 586, 787
EcoRII CCWGG 1 cut(s) 625
EcoT14I CCWWGG 2 cut(s) 597, 879
EcoT38I GRGCYC 3 cut(s) 368, 539, 1079
ErhI CCWWGG 2 cut(s) 597, 879
Esp3I CGTCTC 1 cut(s) 608
FaeI CATG 2 cut(s) 289, 799
FaiI YATR 6 cut(s) 191, 287, 573, 797, 954, 1001
FaqI GGGAC 3 cut(s) 264, 599, 668
FatI CATG 2 cut(s) 285, 795
FauI CCCGC 2 cut(s) 64, 399
FblI GTMKAC 2 cut(s) 672, 692
Fnu4HI GCNGC 4 cut(s) 8, 225, 378, 509
FokI GGATG 2 cut(s) 770, 995
FriOI GRGCYC 3 cut(s) 368, 539, 1079
Fsp4HI GCNGC 4 cut(s) 8, 225, 378, 509
FspBI CTAG 1 cut(s) 894
GluI GCNGC 4 cut(s) 8, 225, 378, 509
GsuI CTGGAG 1 cut(s) 741
HaeIII GGCC 4 cut(s) 69, 314, 335, 488
HapII CCGG 6 cut(s) 90, 162, 248, 273, 311, 465
HgaI GACGC 1 cut(s) 38
Hin1I GRCGYC 2 cut(s) 30, 669
Hin1II CATG 2 cut(s) 289, 799
HincII GTYRAC 2 cut(s) 673, 693
HindII GTYRAC 2 cut(s) 673, 693
HinfI GANTC 8 cut(s) 24, 148, 239, 252, 324, 656, 689, 694
HpaII CCGG 6 cut(s) 90, 162, 248, 273, 311, 465
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 3 cut(s) 673, 679, 693
Hpy188I TCNGA 6 cut(s) 64, 238, 244, 494, 578, 990
Hpy188III TCNNGA 6 cut(s) 21, 215, 436, 465, 720, 894
Hpy8I GTNNAC 3 cut(s) 673, 679, 693
Hpy99I CGWCG 7 cut(s) 32, 68, 101, 446, 494, 674, 677
HpyAV CCTTC 2 cut(s) 1007, 1049
HpyCH4III ACNGT 3 cut(s) 49, 842, 1032
HpyCH4IV ACGT 3 cut(s) 503, 669, 675
HpyCH4V TGCA 2 cut(s) 408, 1094
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 457, 505
HpyF3I CTNAG 4 cut(s) 367, 412, 452, 987
HpySE526I ACGT 3 cut(s) 503, 669, 675
Hsp92I GRCGYC 2 cut(s) 30, 669
Hsp92II CATG 2 cut(s) 289, 799
KflI GGGWCCC 1 cut(s) 586
Kpn2I TCCGGA 1 cut(s) 464
KpnI GGTACC 1 cut(s) 96
Kzo9I GATC 9 cut(s) 36, 81, 111, 168, 233, 294, 417, 438, 760
LmnI GCTCC 4 cut(s) 107, 534, 542, 639
Lsp1109I GCAGC 1 cut(s) 495
LweI GCATC 2 cut(s) 417, 973
MaeI CTAG 1 cut(s) 894
MaeII ACGT 3 cut(s) 503, 669, 675
MaeIII GTNAC 4 cut(s) 40, 340, 541, 842
MalI GATC 9 cut(s) 38, 83, 113, 170, 235, 296, 419, 440, 762
MboI GATC 9 cut(s) 36, 81, 111, 168, 233, 294, 417, 438, 760
MboII GAAGA 5 cut(s) 319, 364, 490, 1058, 1077
MflI RGATCY 1 cut(s) 233
MhlI GDGCHC 3 cut(s) 368, 539, 1079
MluCI AATT 3 cut(s) 748, 834, 961
MlyI GAGTC 4 cut(s) 246, 650, 688, 698
MmeI TCCRAC 3 cut(s) 87, 267, 472
MroI TCCGGA 1 cut(s) 464
MseI TTAA 1 cut(s) 807
MslI CAYNNNNRTG 1 cut(s) 413
MspI CCGG 6 cut(s) 90, 162, 248, 273, 311, 465
MspR9I CCNGG 4 cut(s) 248, 249, 311, 627
MvaI CCWGG 1 cut(s) 627
MwoI GCNNNNNNNGC 3 cut(s) 86, 457, 505
NciI CCSGG 3 cut(s) 248, 249, 311
NdeII GATC 9 cut(s) 36, 81, 111, 168, 233, 294, 417, 438, 760
NlaIII CATG 2 cut(s) 289, 799
NlaIV GGNNCC 7 cut(s) 94, 103, 235, 587, 588, 789, 877
NmeAIII GCCGAG 5 cut(s) 56, 230, 387, 452, 927
NmuCI GTSAC 2 cut(s) 40, 541
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 381
PfeI GAWTC 4 cut(s) 24, 148, 239, 324
PkrI GCNGC 4 cut(s) 9, 226, 379, 510
Ple19I CGATCG 1 cut(s) 39
PleI GAGTC 4 cut(s) 246, 650, 688, 697
PpsI GAGTC 4 cut(s) 246, 650, 688, 697
PpuMI RGGWCCY 2 cut(s) 586, 787
PsiI TTATAA 1 cut(s) 1001
Psp124BI GAGCTC 3 cut(s) 368, 539, 1079
Psp5II RGGWCCY 2 cut(s) 586, 787
Psp6I CCWGG 1 cut(s) 625
PspGI CCWGG 1 cut(s) 625
PspN4I GGNNCC 7 cut(s) 94, 103, 235, 587, 588, 789, 877
PspPI GGNCC 5 cut(s) 303, 334, 586, 594, 787
PspPPI RGGWCCY 2 cut(s) 586, 787
PsrI GAACNNNNNNTAC 2 cut(s) 157, 189
PsuI RGATCY 1 cut(s) 233
PvuI CGATCG 1 cut(s) 39
RsaI GTAC 4 cut(s) 51, 94, 175, 563
RsaNI GTAC 4 cut(s) 50, 93, 174, 562
RseI CAYNNNNRTG 1 cut(s) 413
SacI GAGCTC 3 cut(s) 368, 539, 1079
SalI GTCGAC 2 cut(s) 671, 691
SaqAI TTAA 1 cut(s) 807
SatI GCNGC 4 cut(s) 8, 225, 378, 509
Sau3AI GATC 9 cut(s) 36, 81, 111, 168, 233, 294, 417, 438, 760
Sau96I GGNCC 5 cut(s) 303, 334, 586, 594, 787
SchI GAGTC 4 cut(s) 246, 650, 688, 698
ScrFI CCNGG 4 cut(s) 248, 249, 311, 627
SduI GDGCHC 3 cut(s) 368, 539, 1079
SfaNI GCATC 2 cut(s) 417, 973
SgrDI CGTCGACG 1 cut(s) 671
SinI GGWCC 4 cut(s) 303, 586, 594, 787
SmaI CCCGGG 1 cut(s) 249
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 3 cut(s) 748, 834, 961
SspMI CTAG 1 cut(s) 894
SstI GAGCTC 3 cut(s) 368, 539, 1079
StyD4I CCNGG 4 cut(s) 246, 247, 309, 625
StyI CCWWGG 2 cut(s) 597, 879
TaaI ACNGT 3 cut(s) 49, 842, 1032
TaiI ACGT 3 cut(s) 506, 672, 678
TaqI TCGA 9 cut(s) 27, 35, 197, 293, 384, 420, 672, 692, 1011
TaqII GACCGA 1 cut(s) 320
TasI AATT 3 cut(s) 748, 834, 961
TatI WGTACW 1 cut(s) 49
TauI GCSGC 3 cut(s) 10, 227, 380
TfiI GAWTC 4 cut(s) 24, 148, 239, 324
Tru1I TTAA 1 cut(s) 807
Tru9I TTAA 1 cut(s) 807
TscAI CASTG 2 cut(s) 305, 845
TseFI GTSAC 2 cut(s) 40, 541
TseI GCWGC 1 cut(s) 508
Tsp45I GTSAC 2 cut(s) 40, 541
TspGWI ACGGA 4 cut(s) 94, 161, 529, 753
TspMI CCCGGG 1 cut(s) 247
TspRI CASTG 2 cut(s) 305, 845
VpaK11BI GGWCC 4 cut(s) 303, 586, 594, 787
XapI RAATTY 1 cut(s) 961
XbaI TCTAGA 1 cut(s) 893
XmaI CCCGGG 1 cut(s) 247
XmiI GTMKAC 2 cut(s) 672, 692
XspI CTAG 1 cut(s) 894
ZraI GACGTC 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.