Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0012589

Physical Location & Seq

Genomic Coordinates Reverse (-)

6788045 .. 6795564

Gene Structure 7520 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G006620.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3048 bp

ATGTCGATTCTACCCTCGCGCTTCCTCACCTTCCACCACCGACCAACCCTCGCATTCTCACCCTTCCTGCCATCATCACCGCCGTCGCCGTCGTCCATCTTCTACGCGCCGACGAACAATGTCTCCAGACCTCTACGCGCCGAACAATGTCTCCAACCGCTCTCCGCCGCGTCATCCTCCTCCTCCTCCTCGGTCACGGTGGAAAACTCCGAGGAATACTACTCCGAATCCGACGGAGCCGCCGCGTCCGCCACCACGCAGTTCGTGATCCGCGCCCGCAACCGCATCGGCCTCCTCGGGATCATCACGCGCGTGTTCGACGTCCTCGGCCTCCTCGTCGACAGGGCCACCGTGGAATTCGAAGGCGACTACTTCGTGAAGCGGTTCTTCGTCACCGACTCCCGAGGCGCGAGGATCGAGGACCCGGAGAGCCTGGACCGGATCCAGAAGGCGTTACTGGACGCCATCGACGACTGCGGCGGGTCCGTCACCGCGGGGCCTACCACCACCACGAGAGGCGTCGTGGTCCGCAGGCCGGGTTTGGGCCTGGGCTCCGGCCAGCGCGGCGCTAAGGCGGAGAGGATGTTCGGGTTGATGGACGGGTTCTTGAAAAACGACCCGGTTAGTCTTCAGAAGGATATTCTCTACCACGTTGAGTACACCGTGGCCAGGTCGAGGTTTAGCTTTGATGATTTTGAAGCTTATCAGGCTTTAGCGCACAGTGTAAGAGATCGCTTGATTGAGCGTTCGCATGATACTCAGCTTTACTTCAAGAGGAAAGATCCGAAGCGCGTGTACTTCCTCTCGCTTGAGTTTCTTATGGGTCGTTCATTGTCGAATAGTGTCATCAACCTTGGTATCCGAGACCAATACGCAGAGGCTTTAAGTCAGCTTGGCTTTGAGTTTGAAGTTCTGGCCGAGCAGGAAGGAGATGCTGCCCTTGGTAATGGTGGCCTAGCCCGTCTTTCAGCTTGCCAAATGGATTCTATGGCAACTTTGGATTATCCTGCATGGGGTTATGGACTACGCTACCAATATGGACTATTCCGCCAGGTTATACTGGATGGCATTCAGCATGAACAACCTGACTTCTGGTTGAACTTTGGGAATCCCTGGGAGACAGAGCGGGTTCATGTAACATATCCAGTGAAGTTCTATGGGACTGTTGAAGAGGAAATCTTGAACGGAGAAAAATGTAATGTTTGGAACCCTGGAGAATTGGTAGAAGCTGTGGCTTATGATAATCCTATACCTGGTTACGGAACCAGGAACACCATTACTCTCCGCTTATGGGCCGGAAAACCAAGCGATCAACGTGATATGGAAGCTTTTAATACTGGAGATTACATCAATGCTCTTGTTTCTAGACAGAAAGCAGAAAATATAAGTAGTGTTTTGTACCCTGATGATCGTTCTTATCAGGGGAAGGAACTGCGGTTAAAACAACAATATTTCTTTGTCTCAGCATCCATACAAGACATAATACGGAGGTTCAAAGATGCTCACAGTAATTTTGATGAATTTCCTGATAAGGTTGCTCTGCAGCTCAATGATACTCACCCATCTCTTGCAATAGTCGAGGTCATGAGAGTGCTTGTCGATGAAGAGCATTTGGATTGGAATAAAGCTTGGGATATTGTTTGCAAACTCTTTTCTTTCACAATACACGCAGTAATTGCTGAAGGGTTGGAGAAAATCCCTGTTGATTTACTGGGTAGTCTTCTCCCACGCCATTTACAAATTATATATGACATTAACTTCAATTTTGTCGAAGAGTTGAAGAAGAGGATTGGTTTAGATTATGATCGTCTCTCTCGGATGTCTATTGTTGAGGAAGCTGCTGTAAAGAGTATCCGAATGGCAAACCTGTCGGTTGTGTGTGCTCATACTGTGAATGGTGTTTCCACAGTACATTCAGAACTATTAAAAACAAAATTATTCAAGGACTTCTATGAACTGTGGCCTGAGAAATTTCAATGCATGACAAATGGCGTCACCCAGCGCCGCTGGATTGTGGTGAGTAATCCTAGCCTGTGTGCTCTTCTCTCAAAATGGCTTGGAACAGAAGCCTGGATTCGTAATGTTGACCTTCTGACTGGCTTGAGAGAATATGCAGATGATGCTGATCTTCAACAAGAATGGATGATGGTTAAGAAGGTTAACAAGATGAGGCTTGCTGAGTACATTGAAGCAATGAGTGGTGTGAAGGTTAGTTTAGATGCAATGTTTGATGTGCAGACAAAGAGAATACACGAGTACAAAAGACAATTGCTCAATATACTTGGCATCATTCATCGATATGACTGTATCAAGAACATGGATAAGAGTCAACAGAGCAAGGTTGTACCCCGTGTCTGCATTATTGGAGGAAAAGCTGCTCCTGGTTATGAAGTTGCAAAGAAGATTATCAAACTTTGTCATGCTGTAGCAGAAAAGATAAATAATGATAGCGATGTTGGGGATCTTCTAAAGCTGGTTTTTATTCCTGATTATAATGTATCGCTCGCTGAAGTGGTAATACCAGGGGCTGACCTTTCCCAGCACTTGAGCACCGCAGGACATGAGGCTTCTGGAACTGGCAGCATGAAATTTCTCATGAACGGATGTTTACTCTTGGCTACAGAAGATGGGTCTACAGTTGAAATTGTTGAAGAAATAGGGGCAGAGAACTTGTTTTTGTTTGGTGCAAAGATTCATGAAGTTCCTGATTTGCGTGAGAAAGGACCTGCTCATGACGTGCCACTGCAATGTGCACGCGTTTTAAGGCTGATCCGAGATGGTCATTTTGGCTTTCAAGACTATTTCCAATCTCTATGTGATAGTGTAGAGGGTGACGACTTCTATCTGCTCAGCTCTGACTTTGGGAGCTATTTAGAGGCACAGGCTGCTGCTGATAAAGCATTTGCGGATCCAAAGAAGTGGACTAAAATGAGCATCCTTAGCACAGCCGGTTCTGGTAGATTTAGCAGTGACAGAACAATCCGCGACTATGCTGAGAAGAGTTGGGGAATTGAACCGTGCCGATTCCCTTCCGAGTTCGACGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004645 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005980 GO:0006073 GO:0006091 GO:0006112 GO:0008144 GO:0008150 GO:0008152 GO:0008184 GO:0009056 GO:0009057 GO:0009251 GO:0009987 GO:0010033 GO:0010243 GO:0014070 GO:0014074 GO:0015980 GO:0016052 GO:0016740 GO:0016757 GO:0016758 GO:0019842 GO:0030170 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044464 GO:0046683 GO:0048037 GO:0050662 GO:0050896 GO:0051591 GO:0055114 GO:0070279 GO:0071704 GO:0097159 GO:1901363 GO:1901575 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 ko04217 ko04910 ko04922 ko04931 map00500 map01100 map01110 map02026 map04217 map04910 map04922 map04931

Pfam Domains

Protein Families

Phosphorylase

Protein Analysis

1015

Amino Acids

114.14

Weight (kDa)

5.51

Isoelectric Point (pI)

41.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phosphorylase	PF00343	295 - 1004	4.2e-298	Carbohydrate phosphorylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012589)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g08650 FvH4_3g08650 FvH4_3g08650 FvH4_3g08650 FvH4_3g08650
malus_domestica	MD10G1289300.v1.1
prunus_persica	Prupe.4G052400_v2.0.a1
pyrus_communis	pycom10g24190
rosa_chinensis	RchiOBHm_Chr5g0009141
rosa_laevigata	RLG00000031633
rosa_multiflora	Rmu_sc0005923.1_g000005
rosa_roxburghii	Rroxscaffold_1G00066860 Rroxscaffold_1G00067560
rosa_rugosa	Rorug04G0441500
rosa_samantha	Rh5AG070800 Rh5BG067700 Rh5CG079600 Rh5DG067400
rosa_wichuraiana	Rw5G006620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2493
AatII	GACGTC	1 cut(s)	324
Acc36I	ACCTGC	1 cut(s)	2734
AccBSI	CCGCTC	2 cut(s)	160, 1126
AccI	GTMKAC	2 cut(s)	339, 2633
AcoI	YGGCCR	3 cut(s)	556, 666, 915
AcsI	RAATTY	4 cut(s)	356, 1520, 1970, 2588
AcuI	CTGAAG	3 cut(s)	614, 1701, 2529
AcyI	GRCGYC	4 cut(s)	321, 462, 519, 1992
AfaI	GTAC	7 cut(s)	659, 797, 1400, 1911, 2183, 2258, 2346
AfiI	CCNNNNNNNGG	8 cut(s)	535, 542, 669, 675, 1013, 1253, 1259, 1291
AflIII	ACRYGT	1 cut(s)	2755
AjiI	CACGTC	1 cut(s)	2737
AleI	CACNNNNGTG	1 cut(s)	311
AloI	GAACNNNNNNTCC	6 cut(s)	107, 135, 139, 167, 569, 601
Alw21I	GWGCWC	4 cut(s)	1885, 2041, 2552, 2755
Alw26I	GTCTC	6 cut(s)	127, 155, 858, 1112, 1465, 1814
Alw44I	GTGCAC	1 cut(s)	2751
AlwNI	CAGNNNCTG	1 cut(s)	2528
Ama87I	CYCGRG	2 cut(s)	296, 402
ApaLI	GTGCAC	1 cut(s)	2751
ApeKI	GCWGC	7 cut(s)	935, 1543, 1838, 2375, 2580, 2885, 2888
ApoI	RAATTY	4 cut(s)	356, 1520, 1970, 2588
Asp700I	GAANNNNTTC	1 cut(s)	383
AspS9I	GGNCC	9 cut(s)	345, 421, 436, 483, 497, 526, 544, 1293, 2723
AsuC2I	CCSGG	3 cut(s)	425, 537, 620
AsuHPI	GGTGA	9 cut(s)	19, 51, 69, 385, 481, 1550, 1987, 2029, 2843
AsuII	TTCGAA	1 cut(s)	360
AvaI	CYCGRG	2 cut(s)	296, 402
AvaII	GGWCC	5 cut(s)	421, 436, 483, 526, 2723
BaeGI	GKGCMC	1 cut(s)	2755
BaeI	ACNNNNGTAYC	2 cut(s)	841, 874
BalI	TGGCCA	1 cut(s)	668
BamHI	GGATCC	2 cut(s)	441, 2908
BanII	GRGCYC	1 cut(s)	554
BarI	GAAGNNNNNNTAC	4 cut(s)	779, 811, 2502, 2534
BauI	CACGAG	2 cut(s)	511, 2252
BbsI	GAAGAC	2 cut(s)	620, 1712
Bbv12I	GWGCWC	4 cut(s)	1885, 2041, 2552, 2755
BbvI	GCAGC	7 cut(s)	922, 1555, 1825, 2362, 2592, 2872, 2875
BccI	CCATC	9 cut(s)	79, 104, 473, 589, 1058, 1570, 2140, 2621, 2771
BceAI	ACGGC	2 cut(s)	67, 73
BcgI	CGANNNNNNTGC	4 cut(s)	268, 302, 1851, 1885
BciVI	GTATCC	2 cut(s)	869, 1862
BcnI	CCSGG	3 cut(s)	425, 537, 620
BcoDI	GTCTC	6 cut(s)	127, 155, 858, 1112, 1465, 1814
BfaI	CTAG	4 cut(s)	956, 1365, 2028, 3046
BfmI	CTRYAG	4 cut(s)	1541, 2424, 2619, 2634
BfoI	RGCGCY	2 cut(s)	570, 2005
BfuAI	ACCTGC	1 cut(s)	2734
BfuI	GTATCC	2 cut(s)	869, 1862
BglI	GCCNNNNNGGC	1 cut(s)	564
BlpI	GCTNAGC	1 cut(s)	2849
Bme18I	GGWCC	5 cut(s)	421, 436, 483, 526, 2723
BmeT110I	CYCGRG	2 cut(s)	296, 402
BmgBI	CACGTC	1 cut(s)	2737
BmgT120I	GGNCC	9 cut(s)	345, 421, 436, 483, 497, 526, 544, 1293, 2723
BmiI	GGNNCC	9 cut(s)	238, 423, 443, 484, 498, 553, 1208, 1264, 2910
BmrI	ACTGGG	1 cut(s)	1721
BmsI	GCATC	8 cut(s)	294, 922, 1475, 1489, 2110, 2209, 2295, 2943
BmuI	ACTGGG	1 cut(s)	1721
BpiI	GAAGAC	2 cut(s)	620, 1712
BpmI	CTGGAG	3 cut(s)	109, 1233, 1359
Bpu10I	CCTNAGC	2 cut(s)	570, 2939
Bpu1102I	GCTNAGC	1 cut(s)	2849
Bpu14I	TTCGAA	1 cut(s)	360
BpuEI	CTTGAG	3 cut(s)	830, 2122, 2566
BpuMI	CCSGG	3 cut(s)	425, 537, 620
Bsa29I	ATCGAT	1 cut(s)	2296
BsaBI	GATNNNNATC	2 cut(s)	1803, 2124
BsaHI	GRCGYC	4 cut(s)	321, 462, 519, 1992
BsaI	GGTCTC	1 cut(s)	858
BsaWI	WCCGGW	1 cut(s)	438
BsaXI	ACNNNNNCTCC	8 cut(s)	107, 135, 137, 165, 569, 599, 1206, 1236
Bsc4I	CCNNNNNNNGG	8 cut(s)	535, 542, 669, 675, 1013, 1253, 1259, 1291
Bse118I	RCCGGY	1 cut(s)	2948
Bse1I	ACTGG	7 cut(s)	462, 1065, 1145, 1342, 1716, 2101, 2581
Bse3DI	GCAATG	3 cut(s)	2199, 2229, 2753
Bse8I	GATNNNNATC	2 cut(s)	1803, 2124
BseCI	ATCGAT	1 cut(s)	2296
BseGI	GGATG	8 cut(s)	173, 588, 1069, 1466, 1824, 2148, 2609, 2934
BseJI	GATNNNNATC	2 cut(s)	1803, 2124
BseLI	CCNNNNNNNGG	8 cut(s)	535, 542, 669, 675, 1013, 1253, 1259, 1291
BseMI	GCAATG	3 cut(s)	2199, 2229, 2753
BseMII	CTCAG	6 cut(s)	773, 1476, 1956, 2169, 2863, 2985
BseNI	ACTGG	7 cut(s)	462, 1065, 1145, 1342, 1716, 2101, 2581
BseRI	GAGGAG	6 cut(s)	169, 172, 175, 178, 284, 323
BseSI	GKGCMC	1 cut(s)	2755
BseXI	GCAGC	7 cut(s)	922, 1555, 1825, 2362, 2592, 2872, 2875
BseYI	CCCAGC	2 cut(s)	1998, 2538
BsgI	GTGCAG	1 cut(s)	2255
BshVI	ATCGAT	1 cut(s)	2296
BsiHKAI	GWGCWC	4 cut(s)	1885, 2041, 2552, 2755
BsiHKCI	CYCGRG	2 cut(s)	296, 402
BsiSI	CCGG	7 cut(s)	425, 439, 536, 555, 620, 1296, 2949
BslFI	GGGAC	1 cut(s)	1174
BslI	CCNNNNNNNGG	8 cut(s)	535, 542, 669, 675, 1013, 1253, 1259, 1291
BsmAI	GTCTC	6 cut(s)	127, 155, 858, 1112, 1465, 1814
BsmBI	CGTCTC	1 cut(s)	1814
BsmFI	GGGAC	1 cut(s)	1174
BsmI	GAATGC	2 cut(s)	53, 1068
Bso31I	GGTCTC	1 cut(s)	858
BsoBI	CYCGRG	2 cut(s)	296, 402
Bsp119I	TTCGAA	1 cut(s)	360
Bsp1286I	GDGCHC	5 cut(s)	554, 1885, 2041, 2552, 2755
Bsp1720I	GCTNAGC	1 cut(s)	2849
BspCNI	CTCAG	6 cut(s)	772, 1475, 1957, 2170, 2862, 2986
BspDI	ATCGAT	1 cut(s)	2296
BspHI	TCATGA	4 cut(s)	1584, 2595, 2695, 2731
BspLI	GGNNCC	9 cut(s)	238, 423, 443, 484, 498, 553, 1208, 1264, 2910
BspMAI	CTGCAG	1 cut(s)	1545
BspMI	ACCTGC	1 cut(s)	2734
BspQI	GCTCTTC	2 cut(s)	1599, 2046
BspT104I	TTCGAA	1 cut(s)	360
BspTNI	GGTCTC	1 cut(s)	858
BsrBI	CCGCTC	2 cut(s)	160, 1126
BsrDI	GCAATG	3 cut(s)	2199, 2229, 2753
BsrFI	RCCGGY	1 cut(s)	2948
BsrI	ACTGG	7 cut(s)	462, 1065, 1145, 1342, 1716, 2101, 2581
BssAI	RCCGGY	1 cut(s)	2948
BssNI	GRCGYC	4 cut(s)	321, 462, 519, 1992
BssSI	CACGAG	2 cut(s)	511, 2252
BssT1I	CCWWGG	2 cut(s)	853, 940
Bst2BI	CACGAG	2 cut(s)	511, 2252
Bst6I	CTCTTC	6 cut(s)	1164, 1599, 1767, 1778, 2046, 2993
BstACI	GRCGYC	4 cut(s)	321, 462, 519, 1992
BstAPI	GCANNNNNTGC	3 cut(s)	1676, 2120, 2885
BstBI	TTCGAA	1 cut(s)	360
BstC8I	GCNNGC	7 cut(s)	277, 533, 560, 973, 2175, 2505, 2755
BstDEI	CTNAG	8 cut(s)	570, 759, 1462, 1965, 2178, 2849, 2939, 2994
BstDSI	CCRYGG	3 cut(s)	351, 492, 663
BstF5I	GGATG	8 cut(s)	173, 588, 1069, 1466, 1824, 2148, 2609, 2934
BstH2I	RGCGCY	2 cut(s)	570, 2005
BstMAI	GTCTC	6 cut(s)	127, 155, 858, 1112, 1465, 1814
BstMWI	GCNNNNNNNGC	8 cut(s)	248, 564, 707, 1676, 2120, 2885, 2897, 2940
BstSFI	CTRYAG	4 cut(s)	1541, 2424, 2619, 2634
BstSLI	GKGCMC	1 cut(s)	2755
BstV1I	GCAGC	7 cut(s)	922, 1555, 1825, 2362, 2592, 2872, 2875
BstV2I	GAAGAC	2 cut(s)	620, 1712
BstX2I	RGATCY	4 cut(s)	441, 781, 2461, 2908
BstXI	CCANNNNNNTGG	1 cut(s)	2919
BstYI	RGATCY	4 cut(s)	441, 781, 2461, 2908
Bsu15I	ATCGAT	1 cut(s)	2296
BsuI	GTATCC	2 cut(s)	869, 1862
BsuTUI	ATCGAT	1 cut(s)	2296
BtgI	CCRYGG	3 cut(s)	351, 492, 663
BtgZI	GCGATG	1 cut(s)	2466
BtrI	CACGTC	1 cut(s)	2737
BtsCI	GGATG	8 cut(s)	173, 588, 1069, 1466, 1824, 2148, 2609, 2934
BtsI	GCAGTG	2 cut(s)	2741, 2974
BtsIMutI	CAGTG	4 cut(s)	727, 1152, 2741, 2974
BveI	ACCTGC	1 cut(s)	2734
Cac8I	GCNNGC	7 cut(s)	277, 533, 560, 973, 2175, 2505, 2755
CaiI	CAGNNNCTG	1 cut(s)	2528
CciI	TCATGA	4 cut(s)	1584, 2595, 2695, 2731
Cfr10I	RCCGGY	1 cut(s)	2948
Cfr13I	GGNCC	9 cut(s)	345, 421, 436, 483, 497, 526, 544, 1293, 2723
Cfr42I	CCGCGG	1 cut(s)	495
ClaI	ATCGAT	1 cut(s)	2296
CseI	GACGC	5 cut(s)	159, 234, 470, 508, 1981
CsiI	ACCWGGT	1 cut(s)	1252
Csp6I	GTAC	7 cut(s)	658, 796, 1399, 1910, 2182, 2257, 2345
CviQI	GTAC	7 cut(s)	658, 796, 1399, 1910, 2182, 2257, 2345
DdeI	CTNAG	8 cut(s)	570, 759, 1462, 1965, 2178, 2849, 2939, 2994
EaeI	YGGCCR	3 cut(s)	556, 666, 915
Eam1104I	CTCTTC	6 cut(s)	1164, 1599, 1767, 1778, 2046, 2993
EarI	CTCTTC	6 cut(s)	1164, 1599, 1767, 1778, 2046, 2993
EciI	GGCGGA	4 cut(s)	154, 238, 590, 1037
Eco130I	CCWWGG	2 cut(s)	853, 940
Eco24I	GRGCYC	1 cut(s)	554
Eco31I	GGTCTC	1 cut(s)	858
Eco47I	GGWCC	5 cut(s)	421, 436, 483, 526, 2723
Eco57I	CTGAAG	3 cut(s)	614, 1701, 2529
Eco88I	CYCGRG	2 cut(s)	296, 402
EcoO109I	RGGNCCY	3 cut(s)	421, 497, 2723
EcoRI	GAATTC	1 cut(s)	356
EcoT14I	CCWWGG	2 cut(s)	853, 940
EcoT22I	ATGCAT	1 cut(s)	1982
EcoT38I	GRGCYC	1 cut(s)	554
ErhI	CCWWGG	2 cut(s)	853, 940
Esp3I	CGTCTC	1 cut(s)	1814
FalI	AAGNNNNNCTT	2 cut(s)	371, 403
FaqI	GGGAC	1 cut(s)	1174
FauI	CCCGC	4 cut(s)	284, 473, 487, 1119
FblI	GTMKAC	2 cut(s)	339, 2633
FokI	GGATG	8 cut(s)	160, 595, 1076, 1453, 1831, 2155, 2616, 2921
FriOI	GRGCYC	1 cut(s)	554
FspBI	CTAG	4 cut(s)	956, 1365, 2028, 3046
GsaI	CCCAGC	2 cut(s)	2002, 2542
GsuI	CTGGAG	3 cut(s)	109, 1233, 1359
HaeII	RGCGCY	2 cut(s)	570, 2005
HapII	CCGG	7 cut(s)	425, 439, 536, 555, 620, 1296, 2949
HgaI	GACGC	5 cut(s)	159, 234, 470, 508, 1981
Hin1I	GRCGYC	4 cut(s)	321, 462, 519, 1992
HincII	GTYRAC	4 cut(s)	340, 2086, 2161, 2330
HindII	GTYRAC	4 cut(s)	340, 2086, 2161, 2330
HindIII	AAGCTT	3 cut(s)	699, 1326, 1626
HinfI	GANTC	9 cut(s)	7, 227, 398, 983, 1108, 2074, 2326, 2692, 3024
HpaI	GTTAAC	1 cut(s)	2161
HpaII	CCGG	7 cut(s)	425, 439, 536, 555, 620, 1296, 2949
HphI	GGTGA	9 cut(s)	19, 51, 69, 385, 481, 1550, 1987, 2029, 2843
Hpy99I	CGWCG	9 cut(s)	88, 94, 115, 236, 323, 341, 473, 524, 3044
HpyCH4IV	ACGT	4 cut(s)	321, 651, 1315, 2736
HpyF10VI	GCNNNNNNNGC	8 cut(s)	248, 564, 707, 1676, 2120, 2885, 2897, 2940
HpyF3I	CTNAG	8 cut(s)	570, 759, 1462, 1965, 2178, 2849, 2939, 2994
HpySE526I	ACGT	4 cut(s)	321, 651, 1315, 2736
Hsp92I	GRCGYC	4 cut(s)	321, 462, 519, 1992
KspAI	GTTAAC	1 cut(s)	2161
KspI	CCGCGG	1 cut(s)	495
LguI	GCTCTTC	2 cut(s)	1599, 2046
LmnI	GCTCC	4 cut(s)	236, 557, 2383, 2865
Lsp1109I	GCAGC	7 cut(s)	922, 1555, 1825, 2362, 2592, 2872, 2875
LweI	GCATC	8 cut(s)	294, 922, 1475, 1489, 2110, 2209, 2295, 2943
MabI	ACCWGGT	1 cut(s)	1252
MaeI	CTAG	4 cut(s)	956, 1365, 2028, 3046
MaeII	ACGT	4 cut(s)	321, 651, 1315, 2736
MaeIII	GTNAC	9 cut(s)	193, 391, 453, 487, 1135, 1256, 1993, 2831, 2969
MbiI	CCGCTC	2 cut(s)	160, 1126
MfeI	CAATTG	1 cut(s)	2267
MflI	RGATCY	4 cut(s)	441, 781, 2461, 2908
MhlI	GDGCHC	5 cut(s)	554, 1885, 2041, 2552, 2755
MlsI	TGGCCA	1 cut(s)	668
MluI	ACGCGT	1 cut(s)	2755
MluNI	TGGCCA	1 cut(s)	668
MlyI	GAGTC	2 cut(s)	392, 2335
MmeI	TCCRAC	3 cut(s)	178, 255, 1668
Mox20I	TGGCCA	1 cut(s)	668
Mph1103I	ATGCAT	1 cut(s)	1982
MroXI	GAANNNNTTC	1 cut(s)	383
MscI	TGGCCA	1 cut(s)	668
MseI	TTAA	8 cut(s)	884, 1332, 1439, 1755, 1925, 2151, 2160, 2762
MslI	CAYNNNNRTG	4 cut(s)	311, 1589, 2298, 2746
Msp20I	TGGCCA	1 cut(s)	668
MspA1I	CMGCKG	2 cut(s)	494, 2007
MspI	CCGG	7 cut(s)	425, 439, 536, 555, 620, 1296, 2949
MteI	GCGCNGCGC	1 cut(s)	565
MunI	CAATTG	1 cut(s)	2267
Mva1269I	GAATGC	2 cut(s)	53, 1068
MwoI	GCNNNNNNNGC	8 cut(s)	248, 564, 707, 1676, 2120, 2885, 2897, 2940
NciI	CCSGG	3 cut(s)	425, 537, 620
NlaIV	GGNNCC	9 cut(s)	238, 423, 443, 484, 498, 553, 1208, 1264, 2910
NmeAIII	GCCGAG	2 cut(s)	306, 943
NmuCI	GTSAC	6 cut(s)	193, 391, 487, 1993, 2831, 2969
NsiI	ATGCAT	1 cut(s)	1982
NspV	TTCGAA	1 cut(s)	360
OliI	CACNNNNGTG	1 cut(s)	311
PagI	TCATGA	4 cut(s)	1584, 2595, 2695, 2731
PasI	CCCWGGG	1 cut(s)	1113
PciSI	GCTCTTC	2 cut(s)	1599, 2046
PcsI	WCGNNNNNNNCGW	2 cut(s)	333, 468
PctI	GAATGC	2 cut(s)	53, 1068
PdmI	GAANNNNTTC	1 cut(s)	383
PfeI	GAWTC	7 cut(s)	7, 227, 983, 1108, 2074, 2692, 3024
PleI	GAGTC	2 cut(s)	392, 2334
PpsI	GAGTC	2 cut(s)	392, 2334
PpuMI	RGGWCCY	2 cut(s)	421, 2723
PsiI	TTATAA	1 cut(s)	2493
Psp5II	RGGWCCY	2 cut(s)	421, 2723
PspFI	CCCAGC	2 cut(s)	1998, 2538
PspN4I	GGNNCC	9 cut(s)	238, 423, 443, 484, 498, 553, 1208, 1264, 2910
PspPI	GGNCC	9 cut(s)	345, 421, 436, 483, 497, 526, 544, 1293, 2723
PspPPI	RGGWCCY	2 cut(s)	421, 2723
PstI	CTGCAG	1 cut(s)	1545
PstNI	CAGNNNCTG	1 cut(s)	2528
PsuI	RGATCY	4 cut(s)	441, 781, 2461, 2908
RsaI	GTAC	7 cut(s)	659, 797, 1400, 1911, 2183, 2258, 2346
RsaNI	GTAC	7 cut(s)	658, 796, 1399, 1910, 2182, 2257, 2345
RseI	CAYNNNNRTG	4 cut(s)	311, 1589, 2298, 2746
SacII	CCGCGG	1 cut(s)	495
SalI	GTCGAC	1 cut(s)	338
SapI	GCTCTTC	2 cut(s)	1599, 2046
SaqAI	TTAA	8 cut(s)	884, 1332, 1439, 1755, 1925, 2151, 2160, 2762
Sau96I	GGNCC	9 cut(s)	345, 421, 436, 483, 497, 526, 544, 1293, 2723
SchI	GAGTC	2 cut(s)	392, 2335
SduI	GDGCHC	5 cut(s)	554, 1885, 2041, 2552, 2755
SexAI	ACCWGGT	1 cut(s)	1252
SfaNI	GCATC	8 cut(s)	294, 922, 1475, 1489, 2110, 2209, 2295, 2943
SfcI	CTRYAG	4 cut(s)	1541, 2424, 2619, 2634
Sfr303I	CCGCGG	1 cut(s)	495
SfuI	TTCGAA	1 cut(s)	360
SgrBI	CCGCGG	1 cut(s)	495
SinI	GGWCC	5 cut(s)	421, 436, 483, 526, 2723
SmiMI	CAYNNNNRTG	4 cut(s)	311, 1589, 2298, 2746
SmlI	CTYRAG	3 cut(s)	809, 2101, 2545
SmoI	CTYRAG	3 cut(s)	809, 2101, 2545
SspI	AATATT	1 cut(s)	1451
SspMI	CTAG	4 cut(s)	956, 1365, 2028, 3046
StyI	CCWWGG	2 cut(s)	853, 940
TaiI	ACGT	4 cut(s)	324, 654, 1318, 2739
TaqII	GACCGA	1 cut(s)	181
TatI	WGTACW	5 cut(s)	657, 795, 1909, 2181, 2256
TauI	GCSGC	6 cut(s)	170, 242, 245, 480, 567, 2007
TfiI	GAWTC	7 cut(s)	7, 227, 983, 1108, 2074, 2692, 3024
Tru1I	TTAA	8 cut(s)	884, 1332, 1439, 1755, 1925, 2151, 2160, 2762
Tru9I	TTAA	8 cut(s)	884, 1332, 1439, 1755, 1925, 2151, 2160, 2762
TscAI	CASTG	4 cut(s)	727, 1152, 2748, 2974
TseFI	GTSAC	6 cut(s)	193, 391, 487, 1993, 2831, 2969
TseI	GCWGC	7 cut(s)	935, 1543, 1838, 2375, 2580, 2885, 2888
Tsp45I	GTSAC	6 cut(s)	193, 391, 487, 1993, 2831, 2969
TspGWI	ACGGA	6 cut(s)	249, 475, 1200, 1275, 1501, 2616
TspRI	CASTG	4 cut(s)	727, 1152, 2748, 2974
VneI	GTGCAC	1 cut(s)	2751
VpaK11BI	GGWCC	5 cut(s)	421, 436, 483, 526, 2723
XapI	RAATTY	4 cut(s)	356, 1520, 1970, 2588
XbaI	TCTAGA	1 cut(s)	1364
XmiI	GTMKAC	2 cut(s)	339, 2633
XmnI	GAANNNNTTC	1 cut(s)	383
XspI	CTAG	4 cut(s)	956, 1365, 2028, 3046
ZraI	GACGTC	1 cut(s)	322
Zsp2I	ATGCAT	1 cut(s)	1982

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.