Rw5G013010

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0020124
Physical Location & Seq
Forward (+)
15359966 .. 15363470
3505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G013010.1

Sequence Viewer

Length: 546 bp
ATGCTCAAGACCGACAACTTTTGTTGGACACCAGCAGCAGAGGACGCCTTTGTTCGCCTCAAGCTTGCAGTTACTACCGCCCCAGTCCTTGCCATGCCCGATTTCACCAAACCATTCATGGTAGAAACAAATGCCAGCGGCATCGGAATTGTAAATCAATCTCTTGAACATTATCTCCGGTGTTTCGTCGCGGATAAACCATCCTCCTGGCACAACTTGATCCACTGGGCAGAATGGTGGTACAATACCTCGTACCACTCCACCATCCAAATGACACCCTTCCAAGCCTTATACGGCATCCCTCCACCATCGGTTAATTGCTACCTACCCGGAACCACAGCGGTACATGCAGTGGACACGGCTCTGCGCAATCGTGATGAGCTTCTCCAATCCCTTCGCCATCACATGACCTTAGCTCAAAACCGCATGAAACAACAGGCAGATAAACGCCATACCGATCGAGAGTTCACGATCGGTGATTGGACATCTCCCGATCTCCGCCTCTCCGCGGCTCCGCCCTCTCTCTGCTTCTCGTTTCGATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

181

Amino Acids

20.7

Weight (kDa)

7.08

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH_2 PF17919 9 - 50 9.7e-10 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020124)

Species Orthologous Gene IDs
malus_domestica MD14G1073300.v1.1
pyrus_communis pycom08g08560 pycom16g15850
rosa_wichuraiana Rw1G019750 Rw5G013010 Rw5G048260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 368
AccII CGCG 2 cut(s) 191, 509
AciI CCGC 9 cut(s) 78, 138, 191, 341, 424, 499, 507, 509, 515
AclWI GGATC 1 cut(s) 214
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 3 cut(s) 242, 254, 345
AfiI CCNNNNNNNGG 1 cut(s) 508
AgsI TTSAA 1 cut(s) 167
AjnI CCWGG 1 cut(s) 206
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
AluBI AGCT 3 cut(s) 64, 382, 416
AluI AGCT 3 cut(s) 64, 382, 416
AlwI GGATC 1 cut(s) 214
ApeKI GCWGC 1 cut(s) 35
AspLEI GCGC 1 cut(s) 369
AsuC2I CCSGG 1 cut(s) 330
AsuHPI GGTGA 2 cut(s) 97, 488
BbvI GCAGC 1 cut(s) 47
BccI CCATC 4 cut(s) 208, 272, 316, 408
BceAI ACGGC 2 cut(s) 310, 375
BciT130I CCWGG 1 cut(s) 208
BcnI CCSGG 1 cut(s) 330
BisI GCNGC 3 cut(s) 36, 139, 510
BlsI GCNGC 3 cut(s) 37, 140, 511
Bme1390I CCNGG 2 cut(s) 208, 330
BmiI GGNNCC 2 cut(s) 334, 513
BmrFI CCNGG 2 cut(s) 208, 330
BmrI ACTGGG 2 cut(s) 77, 235
BmsI GCATC 2 cut(s) 150, 306
BmuI ACTGGG 2 cut(s) 77, 235
Bpu10I CCTNAGC 1 cut(s) 412
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 1 cut(s) 507
BsaWI WCCGGW 1 cut(s) 177
BsaXI ACNNNNNCTCC 2 cut(s) 159, 189
Bsc4I CCNNNNNNNGG 1 cut(s) 508
Bse1I ACTGG 2 cut(s) 83, 230
BseBI CCWGG 1 cut(s) 208
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 3 cut(s) 200, 264, 297
BseLI CCNNNNNNNGG 1 cut(s) 508
BseNI ACTGG 2 cut(s) 83, 230
BseXI GCAGC 1 cut(s) 47
Bsh1236I CGCG 2 cut(s) 191, 509
Bsh1285I CGRYCG 2 cut(s) 460, 474
BsiEI CGRYCG 2 cut(s) 460, 474
BsiSI CCGG 2 cut(s) 178, 330
BslI CCNNNNNNNGG 1 cut(s) 508
Bsp143I GATC 4 cut(s) 219, 457, 471, 493
BspACI CCGC 9 cut(s) 78, 138, 191, 341, 424, 499, 507, 509, 515
BspFNI CGCG 2 cut(s) 191, 509
BspLI GGNNCC 2 cut(s) 334, 513
BspPI GGATC 1 cut(s) 214
BsrI ACTGG 2 cut(s) 83, 230
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 4 cut(s) 219, 457, 471, 493
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 208
BstACI GRCGYC 1 cut(s) 45
BstC8I GCNNGC 2 cut(s) 66, 136
BstDEI CTNAG 1 cut(s) 412
BstDSI CCRYGG 1 cut(s) 507
BstF5I GGATG 3 cut(s) 200, 264, 297
BstFNI CGCG 2 cut(s) 191, 509
BstHHI GCGC 1 cut(s) 369
BstKTI GATC 4 cut(s) 222, 460, 474, 496
BstMBI GATC 4 cut(s) 219, 457, 471, 493
BstMCI CGRYCG 2 cut(s) 460, 474
BstMWI GCNNNNNNNGC 2 cut(s) 44, 347
BstNI CCWGG 1 cut(s) 208
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 2 cut(s) 206, 328
BstUI CGCG 2 cut(s) 191, 509
BstV1I GCAGC 1 cut(s) 47
BstXI CCANNNNNNTGG 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 3 cut(s) 200, 264, 297
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 2 cut(s) 223, 357
Cac8I GCNNGC 2 cut(s) 66, 136
CfoI GCGC 1 cut(s) 369
Cfr42I CCGCGG 1 cut(s) 510
CseI GACGC 1 cut(s) 53
Csp6I GTAC 3 cut(s) 241, 253, 344
CviAII CATG 5 cut(s) 94, 118, 347, 406, 427
CviJI RGCY 6 cut(s) 64, 287, 362, 382, 416, 512
CviKI_1 RGCY 6 cut(s) 64, 287, 362, 382, 416, 512
CviQI GTAC 3 cut(s) 241, 253, 344
DdeI CTNAG 1 cut(s) 412
DpnI GATC 4 cut(s) 221, 459, 473, 495
DpnII GATC 4 cut(s) 219, 457, 471, 493
EciI GGCGGA 2 cut(s) 488, 504
EcoRII CCWGG 1 cut(s) 206
FaeI CATG 5 cut(s) 97, 121, 350, 409, 430
FaiI YATR 7 cut(s) 95, 119, 292, 348, 407, 428, 453
FatI CATG 5 cut(s) 93, 117, 346, 405, 426
Fnu4HI GCNGC 3 cut(s) 36, 139, 510
FokI GGATG 3 cut(s) 187, 251, 284
Fsp4HI GCNGC 3 cut(s) 36, 139, 510
FspI TGCGCA 1 cut(s) 368
GlaI GCGC 1 cut(s) 368
GluI GCNGC 3 cut(s) 36, 139, 510
HapII CCGG 2 cut(s) 178, 330
HgaI GACGC 1 cut(s) 53
HhaI GCGC 1 cut(s) 369
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 5 cut(s) 97, 121, 350, 409, 430
Hin6I GCGC 1 cut(s) 367
HinP1I GCGC 1 cut(s) 367
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 1 cut(s) 540
HpaII CCGG 2 cut(s) 178, 330
HphI GGTGA 2 cut(s) 97, 488
Hpy166II GTNNAC 2 cut(s) 355, 468
Hpy188I TCNGA 1 cut(s) 146
Hpy188III TCNNGA 6 cut(s) 7, 164, 374, 461, 469, 491
Hpy8I GTNNAC 2 cut(s) 355, 468
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 2 cut(s) 289, 404
HpyCH4V TGCA 2 cut(s) 68, 350
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 347
HpyF3I CTNAG 1 cut(s) 412
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 5 cut(s) 97, 121, 350, 409, 430
HspAI GCGC 1 cut(s) 367
KspI CCGCGG 1 cut(s) 510
Kzo9I GATC 4 cut(s) 219, 457, 471, 493
LmnI GCTCC 1 cut(s) 517
LpnPI CCDG 9 cut(s) 45, 96, 148, 191, 193, 211, 220, 343, 422
Lsp1109I GCAGC 1 cut(s) 47
LweI GCATC 2 cut(s) 150, 306
MaeIII GTNAC 1 cut(s) 70
MalI GATC 4 cut(s) 221, 459, 473, 495
MboI GATC 4 cut(s) 219, 457, 471, 493
MluCI AATT 2 cut(s) 147, 316
MmeI TCCRAC 1 cut(s) 5
MnlI CCTC 7 cut(s) 34, 68, 214, 259, 312, 512, 529
MseI TTAA 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 3 cut(s) 138, 341, 509
MspI CCGG 2 cut(s) 178, 330
MspR9I CCNGG 2 cut(s) 208, 330
MvaI CCWGG 1 cut(s) 208
MvnI CGCG 2 cut(s) 191, 509
MwoI GCNNNNNNNGC 2 cut(s) 44, 347
NciI CCSGG 1 cut(s) 330
NdeII GATC 4 cut(s) 219, 457, 471, 493
NlaIII CATG 5 cut(s) 97, 121, 350, 409, 430
NlaIV GGNNCC 2 cut(s) 334, 513
NsbI TGCGCA 1 cut(s) 368
NspI RCATGY 1 cut(s) 350
PfeI GAWTC 1 cut(s) 540
PkrI GCNGC 3 cut(s) 37, 140, 511
Ple19I CGATCG 2 cut(s) 460, 474
Psp6I CCWGG 1 cut(s) 206
PspGI CCWGG 1 cut(s) 206
PspN4I GGNNCC 2 cut(s) 334, 513
PvuI CGATCG 2 cut(s) 460, 474
RsaI GTAC 3 cut(s) 242, 254, 345
RsaNI GTAC 3 cut(s) 241, 253, 344
RseI CAYNNNNRTG 1 cut(s) 269
SacII CCGCGG 1 cut(s) 510
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 3 cut(s) 36, 139, 510
Sau3AI GATC 4 cut(s) 219, 457, 471, 493
ScrFI CCNGG 2 cut(s) 208, 330
SetI ASST 6 cut(s) 66, 251, 327, 384, 413, 418
SfaNI GCATC 2 cut(s) 150, 306
Sfr303I CCGCGG 1 cut(s) 510
SgrBI CCGCGG 1 cut(s) 510
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 2 cut(s) 5, 59
SmoI CTYRAG 2 cut(s) 5, 59
Sse9I AATT 2 cut(s) 147, 316
SsiI CCGC 9 cut(s) 78, 138, 191, 341, 424, 499, 507, 509, 515
StyD4I CCNGG 2 cut(s) 206, 328
TaqI TCGA 2 cut(s) 460, 538
TaqII GACCGA 1 cut(s) 26
TasI AATT 2 cut(s) 147, 316
TauI GCSGC 2 cut(s) 141, 512
TfiI GAWTC 1 cut(s) 540
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 2 cut(s) 230, 357
TseI GCWGC 1 cut(s) 35
TspDTI ATGAA 2 cut(s) 106, 443
TspRI CASTG 2 cut(s) 230, 357
XceI RCATGY 1 cut(s) 350
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.