Rw5G048260

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0020124
Physical Location & Seq
Forward (+)
83838177 .. 83840874
2698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048260.1

Sequence Viewer

Length: 540 bp
ATGCTCAAGAACAACAATTTCCTTTGGACACCCATGGCTGAGAAAGCATTCTCTGACCTCAAGACCGCTATCACCTCCGCCCCTGTGCTTGCCTTGCCCAACTTTTCTGCAGAGTTCTTCATCGAGACCGATGCAAGTGGTGAAGGAATTGGGGCCCACTACCTCCGCTGTTTCATCCTGGACAAACCGCACAATTGGTCACATCTCCTTCACTGGGCTGAATGGTGGTACAATTCAACCTTCCACTCAGCCATAAGGATGACCCCATTCCAGGCTGTTTACGGTTACCCACCACCGTCGATCACACAGTACCTACCCGGCACCACCTCTAACAACGCGGTGGACGTGGCCTTGCAAGATCGTGATTCCTTGCTTCGTACACTCAAAGATCACCTGCACATGGCACAAAATCGGATGAAGCAACACTCTGATCCACACCGAACCGAGCGTGAATTTGAAGAGGGAGATTGGTGTTTGATGGACTGGGCCATTGGAGTGGTCGGATTGGGTGTGCAGCCCAAGCCATGTGGGTGGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

179

Amino Acids

20.43

Weight (kDa)

5.95

Isoelectric Point (pI)

26.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH_2 PF17919 9 - 52 1.2e-12 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020124)

Species Orthologous Gene IDs
malus_domestica MD14G1073300.v1.1
pyrus_communis pycom08g08560 pycom16g15850
rosa_wichuraiana Rw1G019750 Rw5G013010 Rw5G048260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 402
Acc36I ACCTGC 1 cut(s) 402
AccB1I GGYRCC 1 cut(s) 320
AccII CGCG 1 cut(s) 338
AciI CCGC 5 cut(s) 66, 78, 166, 188, 338
AclWI GGATC 1 cut(s) 425
AcsI RAATTY 1 cut(s) 452
AfaI GTAC 3 cut(s) 230, 311, 379
AfiI CCNNNNNNNGG 3 cut(s) 214, 271, 400
AgsI TTSAA 2 cut(s) 237, 458
AjiI CACGTC 1 cut(s) 346
AjnI CCWGG 2 cut(s) 177, 270
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 1 cut(s) 425
AoxI GGCC 4 cut(s) 153, 348, 486, 534
ApaI GGGCCC 1 cut(s) 157
ApeKI GCWGC 1 cut(s) 514
ApoI RAATTY 1 cut(s) 452
Asp700I GAANNNNTTC 1 cut(s) 47
AspS9I GGNCC 4 cut(s) 153, 154, 486, 534
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 3 cut(s) 64, 152, 383
BaeGI GKGCMC 1 cut(s) 157
BanI GGYRCC 1 cut(s) 320
BanII GRGCYC 1 cut(s) 157
BbvI GCAGC 1 cut(s) 526
BccI CCATC 1 cut(s) 472
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 2 cut(s) 179, 272
BcnI CCSGG 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 119
BfaI CTAG 1 cut(s) 538
BfmI CTRYAG 1 cut(s) 108
BfuAI ACCTGC 1 cut(s) 402
BisI GCNGC 1 cut(s) 515
BlsI GCNGC 1 cut(s) 516
Bme1390I CCNGG 3 cut(s) 179, 272, 318
BmgBI CACGTC 1 cut(s) 346
BmgT120I GGNCC 4 cut(s) 153, 154, 486, 534
BmiI GGNNCC 3 cut(s) 154, 155, 322
BmrFI CCNGG 3 cut(s) 179, 272, 318
BmrI ACTGGG 2 cut(s) 223, 493
BmsI GCATC 1 cut(s) 121
BmuI ACTGGG 2 cut(s) 223, 493
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 1 cut(s) 33
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 271, 400
Bse1I ACTGG 2 cut(s) 218, 488
BseBI CCWGG 2 cut(s) 179, 272
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 3 cut(s) 174, 264, 420
BseLI CCNNNNNNNGG 3 cut(s) 214, 271, 400
BseMII CTCAG 2 cut(s) 30, 261
BseNI ACTGG 2 cut(s) 218, 488
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 1 cut(s) 526
BsgI GTGCAG 2 cut(s) 380, 533
Bsh1236I CGCG 1 cut(s) 338
BshFI GGCC 4 cut(s) 155, 350, 488, 536
BshNI GGYRCC 1 cut(s) 320
BsiSI CCGG 1 cut(s) 318
BslI CCNNNNNNNGG 3 cut(s) 214, 271, 400
BsmAI GTCTC 1 cut(s) 119
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 4 cut(s) 155, 350, 488, 536
Bso31I GGTCTC 1 cut(s) 119
Bsp120I GGGCCC 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 157
Bsp143I GATC 4 cut(s) 300, 358, 388, 430
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 5 cut(s) 66, 78, 166, 188, 338
BspANI GGCC 4 cut(s) 155, 350, 488, 536
BspCNI CTCAG 2 cut(s) 31, 260
BspFNI CGCG 1 cut(s) 338
BspLI GGNNCC 3 cut(s) 154, 155, 322
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 1 cut(s) 402
BspPI GGATC 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 320
BspTNI GGTCTC 1 cut(s) 119
BsrI ACTGG 2 cut(s) 218, 488
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 4 cut(s) 300, 358, 388, 430
BssT1I CCWWGG 1 cut(s) 33
Bst2UI CCWGG 2 cut(s) 179, 272
Bst4CI ACNGT 3 cut(s) 284, 297, 309
Bst6I CTCTTC 1 cut(s) 453
BstC8I GCNNGC 1 cut(s) 90
BstDEI CTNAG 2 cut(s) 39, 247
BstDSI CCRYGG 1 cut(s) 33
BstEII GGTNACC 1 cut(s) 284
BstF5I GGATG 3 cut(s) 174, 264, 420
BstFNI CGCG 1 cut(s) 338
BstKTI GATC 4 cut(s) 303, 361, 391, 433
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 4 cut(s) 300, 358, 388, 430
BstMWI GCNNNNNNNGC 3 cut(s) 44, 94, 520
BstNI CCWGG 2 cut(s) 179, 272
BstPI GGTNACC 1 cut(s) 284
BstSCI CCNGG 3 cut(s) 177, 270, 316
BstSFI CTRYAG 1 cut(s) 108
BstSLI GKGCMC 1 cut(s) 157
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 1 cut(s) 526
BstXI CCANNNNNNTGG 2 cut(s) 496, 531
BsuRI GGCC 4 cut(s) 155, 350, 488, 536
BtgI CCRYGG 1 cut(s) 33
BtrI CACGTC 1 cut(s) 346
BtsCI GGATG 3 cut(s) 174, 264, 420
BtsIMutI CAGTG 1 cut(s) 211
BveI ACCTGC 1 cut(s) 402
Cac8I GCNNGC 1 cut(s) 90
Cfr13I GGNCC 4 cut(s) 153, 154, 486, 534
Csp6I GTAC 3 cut(s) 229, 310, 378
CspCI CAANNNNNGTGG 1 cut(s) 508
CviAII CATG 3 cut(s) 34, 400, 525
CviQI GTAC 3 cut(s) 229, 310, 378
DdeI CTNAG 2 cut(s) 39, 247
DpnI GATC 4 cut(s) 302, 360, 390, 432
DpnII GATC 4 cut(s) 300, 358, 388, 430
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
EciI GGCGGA 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 157
Eco31I GGTCTC 1 cut(s) 119
Eco91I GGTNACC 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 153
EcoO65I GGTNACC 1 cut(s) 284
EcoRII CCWGG 2 cut(s) 177, 270
EcoT14I CCWWGG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 3 cut(s) 37, 403, 528
FaiI YATR 4 cut(s) 35, 254, 401, 526
FatI CATG 3 cut(s) 33, 399, 524
Fnu4HI GCNGC 1 cut(s) 515
FokI GGATG 3 cut(s) 161, 271, 427
FriOI GRGCYC 1 cut(s) 157
Fsp4HI GCNGC 1 cut(s) 515
FspBI CTAG 1 cut(s) 538
GluI GCNGC 1 cut(s) 515
HaeIII GGCC 4 cut(s) 155, 350, 488, 536
HapII CCGG 1 cut(s) 318
Hin1II CATG 3 cut(s) 37, 403, 528
HinfI GANTC 1 cut(s) 365
HpaII CCGG 1 cut(s) 318
HphI GGTGA 3 cut(s) 64, 152, 383
Hpy166II GTNNAC 3 cut(s) 280, 343, 380
Hpy188I TCNGA 4 cut(s) 55, 414, 430, 503
Hpy188III TCNNGA 4 cut(s) 7, 61, 124, 362
Hpy8I GTNNAC 3 cut(s) 280, 343, 380
Hpy99I CGWCG 1 cut(s) 301
HpyAV CCTTC 3 cut(s) 137, 218, 250
HpyCH4III ACNGT 3 cut(s) 284, 297, 309
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 5 cut(s) 110, 134, 355, 397, 514
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 94, 520
HpyF3I CTNAG 2 cut(s) 39, 247
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 3 cut(s) 37, 403, 528
Kzo9I GATC 4 cut(s) 300, 358, 388, 430
LpnPI CCDG 9 cut(s) 96, 164, 191, 199, 257, 284, 331, 407, 469
Lsp1109I GCAGC 1 cut(s) 526
LweI GCATC 1 cut(s) 121
MaeI CTAG 1 cut(s) 538
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 2 cut(s) 198, 284
MalI GATC 4 cut(s) 302, 360, 390, 432
MboI GATC 4 cut(s) 300, 358, 388, 430
MboII GAAGA 2 cut(s) 109, 470
MfeI CAATTG 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 157
MluCI AATT 5 cut(s) 16, 147, 193, 232, 452
MmeI TCCRAC 1 cut(s) 481
MnlI CCTC 5 cut(s) 68, 85, 173, 337, 454
MroXI GAANNNNTTC 1 cut(s) 47
MslI CAYNNNNRTG 3 cut(s) 257, 494, 529
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 1 cut(s) 318
MspR9I CCNGG 3 cut(s) 179, 272, 318
MunI CAATTG 1 cut(s) 193
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 2 cut(s) 179, 272
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 3 cut(s) 44, 94, 520
NciI CCSGG 1 cut(s) 318
NcoI CCATGG 1 cut(s) 33
NdeII GATC 4 cut(s) 300, 358, 388, 430
NlaIII CATG 3 cut(s) 37, 403, 528
NlaIV GGNNCC 3 cut(s) 154, 155, 322
NmuCI GTSAC 1 cut(s) 198
PaqCI CACCTGC 1 cut(s) 402
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PctI GAATGC 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 1 cut(s) 365
PfoI TCCNGGA 1 cut(s) 177
PkrI GCNGC 1 cut(s) 516
Psp6I CCWGG 2 cut(s) 177, 270
PspEI GGTNACC 1 cut(s) 284
PspGI CCWGG 2 cut(s) 177, 270
PspN4I GGNNCC 3 cut(s) 154, 155, 322
PspOMI GGGCCC 1 cut(s) 153
PspPI GGNCC 4 cut(s) 153, 154, 486, 534
PstI CTGCAG 1 cut(s) 112
RsaI GTAC 3 cut(s) 230, 311, 379
RsaNI GTAC 3 cut(s) 229, 310, 378
RseI CAYNNNNRTG 3 cut(s) 257, 494, 529
SatI GCNGC 1 cut(s) 515
Sau3AI GATC 4 cut(s) 300, 358, 388, 430
Sau96I GGNCC 4 cut(s) 153, 154, 486, 534
ScrFI CCNGG 3 cut(s) 179, 272, 318
SduI GDGCHC 1 cut(s) 157
SetI ASST 8 cut(s) 60, 77, 165, 242, 315, 329, 348, 396
SfaNI GCATC 1 cut(s) 121
SfcI CTRYAG 1 cut(s) 108
SmiMI CAYNNNNRTG 3 cut(s) 257, 494, 529
SmlI CTYRAG 2 cut(s) 5, 59
SmoI CTYRAG 2 cut(s) 5, 59
Sse9I AATT 5 cut(s) 16, 147, 193, 232, 452
SsiI CCGC 5 cut(s) 66, 78, 166, 188, 338
SspMI CTAG 1 cut(s) 538
StyD4I CCNGG 3 cut(s) 177, 270, 316
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 3 cut(s) 284, 297, 309
TaiI ACGT 1 cut(s) 348
TaqI TCGA 2 cut(s) 123, 299
TaqII GACCGA 1 cut(s) 143
TasI AATT 5 cut(s) 16, 147, 193, 232, 452
TfiI GAWTC 1 cut(s) 365
TscAI CASTG 1 cut(s) 218
TseFI GTSAC 1 cut(s) 198
TseI GCWGC 1 cut(s) 514
Tsp45I GTSAC 1 cut(s) 198
TspDTI ATGAA 3 cut(s) 109, 163, 431
TspRI CASTG 1 cut(s) 218
XapI RAATTY 1 cut(s) 452
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.