Rw6G014790
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0018766
Physical Location & Seq
Reverse (-)
27601088 .. 27602665
1578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G014790.1

Sequence Viewer

Length: 1374 bp
ATGGAATTAGAAGATAGGGAAAGTGACACTGAACTGACTCTAGTGAGCTTTGGTGGATTTGGAAGTCATGTTAAAGCAGAAGGTCTTGTAAACTTCTTGGAAGCTGAAATTGGGCTGGTAGATAGGTGTCGGCTGAAAACTTATTTGACCCCTCTAGAGTCTGTTCCGAATTTTGAGCTCATTGATACTGCAAATATTGAAACAATAGATGAATATAAAAAGGTTGAGCCACATGCATTTGTGCATTTTCTTTTGCCTGTGTCAGTGACCGATGTTATGGATGCTGCTGGGCGCTGTCAACTCTTTTTCAACAACCAAGCACTGAAAAATAAGCATGCTGTGATAAAATGTGATTTTAAGGTGGAGTTCCAGGTAAGAGAAATTAGTGAATCCAAAACCTACAAAGAGCTTACATATCTTGTACTTCTGTTGAGGTTGGCTTCATCACCTTGGGTTTCATATCGAACTGCTGATGATGATAGTTATCAATCATTTCCACCATGTTACTTGGATGACGATGTTCCTTGGATTAGAACCACAGATTTCACTCCTAGTGGGGCAATCGGGCGTTGTAATTCTTATAGAGTTTCAATCCCACCGCGGCATGGTGCTGAGTTGAAGAAGGCCATGATTTATCTTGAAGAACAAAGGGTGCAAGTGGATCATATCAAAAAGCCACCTAGGATTCAGGATGAACCTGATTTTGAAAGGCCCATGTCAGATTATTTCTTTTCTATTCATTACAAGGAAAATATACCGTTTGAGATATTGTTCTTGATCACTGCAGTGATGCACAAGGGCATTTTAAATCAGCACCAGTTGTCAGACGGCTTTTTTGATTTACTGGGAAACCAACCAAAGGAGATCATTGTTGCTGCATTGAAACACTTATATTCATACAAATACCCAGTTTTTGATGCCTCTGAAGAGCTGAAACTTGTTCAAGAAAGGTTGCTGACAAACCCCATGCTTATGCACAATGGGATTGCTGAAGTTAGGAGGTTGGTGATTACCCCAACTAAAGCCTATTGTCTTCCACCAGAGAATGGACCATCCAATAGGGTTCTTAGAAAATATAAAGAAGTTGCTAATAGGTTTTTAAGGGTTACCTTTATGGATGAAGGCATGCAAACAATGAATTCCAATGTCATGAATTCTTATGTTGCTCCTATTGTAAAAGAAATCACAGGAAACACCTTCCCTCAGAAAACAAAAGTCTATGCAAGGGTTCAGGACATTCTGTTGAATGGATTTAATTTATGTGATCGTAAGTACTCTTTCTTGGCCTTTTCATCCAACCAACCGAAGTACTGTTATGCCTGGTTTTTCGCTGAAGACGAGAAGATTAACATACAAGGAATGAAAAATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001172 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0003968 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009856 GO:0009875 GO:0009888 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0032774 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0044706 GO:0045087 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048507 GO:0048519 GO:0048544 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090567 GO:0097659 GO:0097747 GO:0098542 GO:0098586 GO:0098727 GO:0099402 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

457

Amino Acids

52.84

Weight (kDa)

5.95

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBD_RDR6 PF24572 11 - 111 1e-25 RNA-dependent RNA polymerase 6, RNA-binding domain
RDR6_2nd PF24577 101 - 218 2.7e-48 RNA-dependent RNA polymerase 6 second domain
PH_RDR2 PF24823 117 - 216 1.5e-07 RDR2 PH-like domain
RdRP_helical PF26252 243 - 324 1.4e-17 RNA dependent RNA polymerase, helical domain
RdRP PF05183 337 - 457 1.6e-22 RNA dependent RNA polymerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018766)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05370 AT2G05370
malus_domestica MD09G1153900.v1.1
rosa_chinensis RchiOBHm_Chr7g0220451
rosa_multiflora Rmu_sc0008004.1_g000005 Rmu_sc0008004.1_g000006
rosa_samantha Rh7AG333200 Rh7CG350700
rosa_wichuraiana Rw6G014790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1046
AccII CGCG 1 cut(s) 601
AciI CCGC 2 cut(s) 599, 601
AclWI GGATC 1 cut(s) 669
AcsI RAATTY 3 cut(s) 169, 1138, 1153
AcuI CTGAAG 3 cut(s) 945, 1011, 1353
AfaI GTAC 3 cut(s) 423, 1274, 1310
AfiI CCNNNNNNNGG 3 cut(s) 605, 859, 1046
AgsI TTSAA 9 cut(s) 200, 310, 591, 619, 641, 707, 883, 944, 1246
AjnI CCWGG 2 cut(s) 369, 1319
AjuI GAANNNNNNNTTGG 3 cut(s) 93, 125, 1351
AleI CACNNNNGTG 1 cut(s) 785
AluBI AGCT 5 cut(s) 48, 104, 178, 409, 931
AluI AGCT 5 cut(s) 48, 104, 178, 409, 931
Alw21I GWGCWC 1 cut(s) 180
AlwI GGATC 1 cut(s) 669
AoxI GGCC 3 cut(s) 624, 710, 1284
ApeKI GCWGC 2 cut(s) 284, 875
ApoI RAATTY 3 cut(s) 169, 1138, 1153
ArsI GACNNNNNNTTYG 2 cut(s) 818, 850
AspA2I CCTAGG 1 cut(s) 680
AspLEI GCGC 1 cut(s) 294
AspS9I GGNCC 2 cut(s) 711, 1049
AsuHPI GGTGA 2 cut(s) 438, 1018
AvaII GGWCC 1 cut(s) 1049
AvrII CCTAGG 1 cut(s) 680
BanII GRGCYC 1 cut(s) 180
BbsI GAAGAC 2 cut(s) 1025, 1341
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 2 cut(s) 271, 862
BccI CCATC 1 cut(s) 1060
BceAI ACGGC 1 cut(s) 844
BciT130I CCWGG 2 cut(s) 371, 1321
BclI TGATCA 1 cut(s) 777
BfaI CTAG 4 cut(s) 41, 155, 552, 681
BfmI CTRYAG 1 cut(s) 783
BfoI RGCGCY 1 cut(s) 295
BisI GCNGC 3 cut(s) 285, 602, 876
BlnI CCTAGG 1 cut(s) 680
BlsI GCNGC 3 cut(s) 286, 603, 877
BmcAI AGTACT 2 cut(s) 1274, 1310
Bme1390I CCNGG 2 cut(s) 371, 1321
Bme18I GGWCC 1 cut(s) 1049
BmgT120I GGNCC 2 cut(s) 711, 1049
BmrFI CCNGG 2 cut(s) 371, 1321
BmrI ACTGGG 2 cut(s) 854, 902
BmsI GCATC 3 cut(s) 271, 780, 907
BmuI ACTGGG 2 cut(s) 854, 902
BpiI GAAGAC 2 cut(s) 1025, 1341
BsaBI GATNNNNATC 1 cut(s) 483
BsaJI CCNNGG 4 cut(s) 449, 524, 599, 680
Bsc4I CCNNNNNNNGG 3 cut(s) 605, 859, 1046
Bse1I ACTGG 3 cut(s) 817, 849, 908
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 2 cut(s) 371, 1321
BseDI CCNNGG 4 cut(s) 449, 524, 599, 680
BseGI GGATG 6 cut(s) 286, 517, 697, 1052, 1123, 1292
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 3 cut(s) 605, 859, 1046
BseMII CTCAG 2 cut(s) 603, 1217
BseNI ACTGG 3 cut(s) 817, 849, 908
BseXI GCAGC 2 cut(s) 271, 862
BseYI CCCAGC 1 cut(s) 287
Bsh1236I CGCG 1 cut(s) 601
BshFI GGCC 3 cut(s) 626, 712, 1286
BsiHKAI GWGCWC 1 cut(s) 180
BslI CCNNNNNNNGG 3 cut(s) 605, 859, 1046
BsnI GGCC 3 cut(s) 626, 712, 1286
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 4 cut(s) 661, 777, 864, 1264
BspACI CCGC 2 cut(s) 599, 601
BspANI GGCC 3 cut(s) 626, 712, 1286
BspCNI CTCAG 2 cut(s) 604, 1216
BspFNI CGCG 1 cut(s) 601
BspHI TCATGA 1 cut(s) 1149
BspMAI CTGCAG 1 cut(s) 787
BspPI GGATC 1 cut(s) 669
BspQI GCTCTTC 1 cut(s) 921
BsrI ACTGG 3 cut(s) 817, 849, 908
BssECI CCNNGG 4 cut(s) 449, 524, 599, 680
BssMI GATC 4 cut(s) 661, 777, 864, 1264
BssT1I CCWWGG 3 cut(s) 449, 524, 680
Bst2UI CCWGG 2 cut(s) 371, 1321
Bst4CI ACNGT 2 cut(s) 759, 1313
Bst6I CTCTTC 1 cut(s) 921
BstC8I GCNNGC 2 cut(s) 336, 1127
BstDEI CTNAG 3 cut(s) 612, 1067, 1203
BstDSI CCRYGG 1 cut(s) 599
BstEII GGTNACC 1 cut(s) 1105
BstF5I GGATG 6 cut(s) 286, 517, 697, 1052, 1123, 1292
BstFNI CGCG 1 cut(s) 601
BstH2I RGCGCY 1 cut(s) 295
BstHHI GCGC 1 cut(s) 294
BstKTI GATC 4 cut(s) 664, 780, 867, 1267
BstMBI GATC 4 cut(s) 661, 777, 864, 1264
BstNI CCWGG 2 cut(s) 371, 1321
BstNSI RCATGY 3 cut(s) 236, 338, 1129
BstPI GGTNACC 1 cut(s) 1105
BstSCI CCNGG 2 cut(s) 369, 1319
BstSFI CTRYAG 1 cut(s) 783
BstUI CGCG 1 cut(s) 601
BstV1I GCAGC 2 cut(s) 271, 862
BstV2I GAAGAC 2 cut(s) 1025, 1341
BsuRI GGCC 3 cut(s) 626, 712, 1286
BtgI CCRYGG 1 cut(s) 599
BtsCI GGATG 6 cut(s) 286, 517, 697, 1052, 1123, 1292
BtsI GCAGTG 2 cut(s) 780, 792
BtsIMutI CAGTG 5 cut(s) 27, 270, 320, 780, 792
Cac8I GCNNGC 2 cut(s) 336, 1127
CciI TCATGA 1 cut(s) 1149
CfoI GCGC 1 cut(s) 294
Cfr13I GGNCC 2 cut(s) 711, 1049
Cfr42I CCGCGG 1 cut(s) 602
Csp6I GTAC 3 cut(s) 422, 1273, 1309
CviQI GTAC 3 cut(s) 422, 1273, 1309
DdeI CTNAG 3 cut(s) 612, 1067, 1203
DpnI GATC 4 cut(s) 663, 779, 866, 1266
DpnII GATC 4 cut(s) 661, 777, 864, 1264
DraI TTTAAA 1 cut(s) 807
Eam1104I CTCTTC 1 cut(s) 921
EarI CTCTTC 1 cut(s) 921
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 3 cut(s) 449, 524, 680
Eco24I GRGCYC 1 cut(s) 180
Eco47I GGWCC 1 cut(s) 1049
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 3 cut(s) 945, 1011, 1353
Eco91I GGTNACC 1 cut(s) 1105
EcoICRI GAGCTC 1 cut(s) 178
EcoO65I GGTNACC 1 cut(s) 1105
EcoRI GAATTC 2 cut(s) 1138, 1153
EcoRII CCWGG 2 cut(s) 369, 1319
EcoT14I CCWWGG 3 cut(s) 449, 524, 680
EcoT22I ATGCAT 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 3 cut(s) 449, 524, 680
FbaI TGATCA 1 cut(s) 777
Fnu4HI GCNGC 3 cut(s) 285, 602, 876
FokI GGATG 6 cut(s) 293, 524, 704, 1039, 1130, 1279
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 3 cut(s) 285, 602, 876
FspBI CTAG 4 cut(s) 41, 155, 552, 681
GlaI GCGC 1 cut(s) 293
GluI GCNGC 3 cut(s) 285, 602, 876
GsaI CCCAGC 1 cut(s) 291
HaeII RGCGCY 1 cut(s) 295
HaeIII GGCC 3 cut(s) 626, 712, 1286
HhaI GCGC 1 cut(s) 294
Hin6I GCGC 1 cut(s) 292
HinP1I GCGC 1 cut(s) 292
HincII GTYRAC 1 cut(s) 299
HindII GTYRAC 1 cut(s) 299
HinfI GANTC 4 cut(s) 37, 158, 389, 685
HphI GGTGA 2 cut(s) 438, 1018
Hpy166II GTNNAC 2 cut(s) 91, 299
Hpy188I TCNGA 5 cut(s) 168, 721, 826, 925, 1206
Hpy188III TCNNGA 7 cut(s) 155, 638, 689, 775, 944, 1150, 1232
Hpy8I GTNNAC 2 cut(s) 91, 299
HpyAV CCTTC 4 cut(s) 74, 616, 1115, 1207
HpyCH4III ACNGT 2 cut(s) 759, 1313
HpyF3I CTNAG 3 cut(s) 612, 1067, 1203
HspAI GCGC 1 cut(s) 292
Ksp22I TGATCA 1 cut(s) 777
KspI CCGCGG 1 cut(s) 602
Kzo9I GATC 4 cut(s) 661, 777, 864, 1264
LguI GCTCTTC 1 cut(s) 921
LmnI GCTCC 1 cut(s) 1171
Lsp1109I GCAGC 2 cut(s) 271, 862
LweI GCATC 3 cut(s) 271, 780, 907
MaeI CTAG 4 cut(s) 41, 155, 552, 681
MaeIII GTNAC 4 cut(s) 23, 265, 503, 1105
MalI GATC 4 cut(s) 663, 779, 866, 1266
MboI GATC 4 cut(s) 661, 777, 864, 1264
MboII GAAGA 7 cut(s) 23, 631, 653, 938, 1025, 1346, 1354
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 9 cut(s) 5, 108, 169, 381, 574, 1138, 1153, 1255, 1366
MlyI GAGTC 2 cut(s) 31, 167
MmeI TCCRAC 1 cut(s) 1320
MnlI CCTC 5 cut(s) 162, 426, 931, 993, 1212
Mph1103I ATGCAT 1 cut(s) 238
MseI TTAA 6 cut(s) 72, 357, 806, 1100, 1254, 1347
MslI CAYNNNNRTG 2 cut(s) 785, 971
MspA1I CMGCKG 1 cut(s) 601
MspR9I CCNGG 2 cut(s) 371, 1321
MvaI CCWGG 2 cut(s) 371, 1321
MvnI CGCG 1 cut(s) 601
NdeII GATC 4 cut(s) 661, 777, 864, 1264
NmuCI GTSAC 2 cut(s) 23, 265
NsiI ATGCAT 1 cut(s) 238
NspI RCATGY 3 cut(s) 236, 338, 1129
OliI CACNNNNGTG 1 cut(s) 785
PaeI GCATGC 2 cut(s) 338, 1129
PagI TCATGA 1 cut(s) 1149
PciSI GCTCTTC 1 cut(s) 921
PcsI WCGNNNNNNNCGW 1 cut(s) 1335
PfeI GAWTC 2 cut(s) 389, 685
PflMI CCANNNNNTGG 1 cut(s) 1046
PkrI GCNGC 3 cut(s) 286, 603, 877
PleI GAGTC 2 cut(s) 31, 166
PpsI GAGTC 2 cut(s) 31, 166
Psp124BI GAGCTC 1 cut(s) 180
Psp6I CCWGG 2 cut(s) 369, 1319
PspEI GGTNACC 1 cut(s) 1105
PspFI CCCAGC 1 cut(s) 287
PspGI CCWGG 2 cut(s) 369, 1319
PspPI GGNCC 2 cut(s) 711, 1049
PstI CTGCAG 1 cut(s) 787
RsaI GTAC 3 cut(s) 423, 1274, 1310
RsaNI GTAC 3 cut(s) 422, 1273, 1309
RseI CAYNNNNRTG 2 cut(s) 785, 971
SacI GAGCTC 1 cut(s) 180
SacII CCGCGG 1 cut(s) 602
SapI GCTCTTC 1 cut(s) 921
SaqAI TTAA 6 cut(s) 72, 357, 806, 1100, 1254, 1347
SatI GCNGC 3 cut(s) 285, 602, 876
Sau3AI GATC 4 cut(s) 661, 777, 864, 1264
Sau96I GGNCC 2 cut(s) 711, 1049
ScaI AGTACT 2 cut(s) 1274, 1310
SchI GAGTC 2 cut(s) 31, 167
ScrFI CCNGG 2 cut(s) 371, 1321
SduI GDGCHC 1 cut(s) 180
SfaNI GCATC 3 cut(s) 271, 780, 907
SfcI CTRYAG 1 cut(s) 783
Sfr303I CCGCGG 1 cut(s) 602
SgrBI CCGCGG 1 cut(s) 602
SinI GGWCC 1 cut(s) 1049
SmiMI CAYNNNNRTG 2 cut(s) 785, 971
SphI GCATGC 2 cut(s) 338, 1129
Sse9I AATT 9 cut(s) 5, 108, 169, 381, 574, 1138, 1153, 1255, 1366
SsiI CCGC 2 cut(s) 599, 601
SspI AATATT 1 cut(s) 196
SspMI CTAG 4 cut(s) 41, 155, 552, 681
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 2 cut(s) 369, 1319
StyI CCWWGG 3 cut(s) 449, 524, 680
TaaI ACNGT 2 cut(s) 759, 1313
TaqI TCGA 1 cut(s) 463
TaqII GACCGA 1 cut(s) 284
TasI AATT 9 cut(s) 5, 108, 169, 381, 574, 1138, 1153, 1255, 1366
TatI WGTACW 3 cut(s) 421, 1272, 1308
TauI GCSGC 1 cut(s) 604
TfiI GAWTC 2 cut(s) 389, 685
Tru1I TTAA 6 cut(s) 72, 357, 806, 1100, 1254, 1347
Tru9I TTAA 6 cut(s) 72, 357, 806, 1100, 1254, 1347
TscAI CASTG 5 cut(s) 34, 270, 327, 787, 792
TseFI GTSAC 2 cut(s) 23, 265
TseI GCWGC 2 cut(s) 284, 875
Tsp45I GTSAC 2 cut(s) 23, 265
TspRI CASTG 5 cut(s) 34, 270, 327, 787, 792
Van91I CCANNNNNTGG 1 cut(s) 1046
VpaK11BI GGWCC 1 cut(s) 1049
XapI RAATTY 3 cut(s) 169, 1138, 1153
XbaI TCTAGA 1 cut(s) 154
XceI RCATGY 3 cut(s) 236, 338, 1129
XmaJI CCTAGG 1 cut(s) 680
XspI CTAG 4 cut(s) 41, 155, 552, 681
ZrmI AGTACT 2 cut(s) 1274, 1310
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.