AT1G03130

Photosystem I reaction center subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014442
Physical Location & Seq
Reverse (-)
753307 .. 754262
956 bp
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UTR
Exon/CDS
Intron
AT1G03130.1

Sequence Viewer

Length: 615 bp
ATGGCAACTCAAGCCGCCGGAATCTTCAGCCCCGCCATAACAACCACTACTTCCGCCGTCAAGAAACTCCACCTCTTCTCATCAAGCCACCGTCCCAAGTCTCTCTCCTTCACCAAAACCGCCATCCGCGCCGAGAAAACAGAGTCCTCCTCTGCTGCCCCAGCCGTGAAAGAAGCTCCAGTTGGATTCACTCCCCCGCAGCTAGACCCAAACACACCATCACCAATCTTCGCCGGAAGCACAGGAGGTCTCCTCCGTAAAGCACAAGTAGAGGAATTTTACGTGATCACATGGAACTCACCGAAAGAACAAATCTTTGAGATGCCAACAGGAGGAGCTGCGATAATGAGAGAAGGACCGAATCTATTGAAACTGGCGAGGAAAGAACAGTGTTTGGCTTTAGGTACGAGGTTGAGGTCAAAGTACAAGATCACTTACCAGTTTTACAGAGTGTTCCCTAACGGAGAGGTTCAATATCTTCACCCGAAAGATGGAGTTTATCCAGAGAAAGCGAATCCAGGAAGAGAAGGTGTTGGACTAAACATGAGATCCATCGGTAAAAATGTTAGTCCCATTGAGGTTAAATTCACTGGGAAACAATCTTATGATTTGTAA

Protein Analysis

204

Amino Acids

22.31

Weight (kDa)

9.78

Isoelectric Point (pI)

47.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaD PF02531 71 - 202 1.1e-67 PsaD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014442)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03130 AT4G02770
fragaria_vesca FvH4_4g15260
malus_domestica MD13G1191300.v1.1 MD16G1191400.v1.1
prunus_persica Prupe.1G011500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0416571
rosa_laevigata RLG00000007973
rosa_multiflora Rmu_sc0003722.1_g000040
rosa_roxburghii Rroxscaffold_5G00360450
rosa_rugosa Rorug04G0143000
rosa_samantha Rh4AG205400 Rh4BG203000 Rh4CG216300 Rh4DG202500
rosa_wichuraiana Rw0G013510 Rw4G017390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 129
AciI CCGC 6 cut(s) 15, 33, 54, 120, 127, 197
AclWI GGATC 1 cut(s) 543
AcsI RAATTY 2 cut(s) 275, 584
AcuI CTGAAG 1 cut(s) 10
AfaI GTAC 2 cut(s) 406, 425
AfiI CCNNNNNNNGG 2 cut(s) 332, 491
AgsI TTSAA 2 cut(s) 370, 473
AjnI CCWGG 1 cut(s) 517
AjuI GAANNNNNNNTTGG 2 cut(s) 165, 197
AluBI AGCT 3 cut(s) 176, 202, 338
AluI AGCT 3 cut(s) 176, 202, 338
Alw26I GTCTC 2 cut(s) 105, 254
AlwI GGATC 1 cut(s) 543
ApeKI GCWGC 3 cut(s) 155, 199, 338
ApoI RAATTY 2 cut(s) 275, 584
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 1 cut(s) 356
AsuHPI GGTGA 4 cut(s) 103, 213, 291, 473
AvaII GGWCC 1 cut(s) 356
BbvI GCAGC 3 cut(s) 142, 211, 325
BccI CCATC 4 cut(s) 131, 226, 485, 560
BceAI ACGGC 2 cut(s) 41, 149
BciT130I CCWGG 1 cut(s) 519
BclI TGATCA 1 cut(s) 285
BcoDI GTCTC 2 cut(s) 105, 254
BfaI CTAG 1 cut(s) 203
BisI GCNGC 4 cut(s) 15, 156, 200, 339
BlsI GCNGC 4 cut(s) 16, 157, 201, 340
Bme1390I CCNGG 1 cut(s) 519
Bme18I GGWCC 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 356
BmrFI CCNGG 1 cut(s) 519
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 600
BplI GAGNNNNNCTC 4 cut(s) 134, 166, 237, 269
BpmI CTGGAG 1 cut(s) 162
BsaAI YACGTR 1 cut(s) 283
BsaI GGTCTC 1 cut(s) 254
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 491
Bse1I ACTGG 4 cut(s) 179, 378, 439, 595
BseBI CCWGG 1 cut(s) 519
BseGI GGATG 1 cut(s) 123
BseLI CCNNNNNNNGG 2 cut(s) 332, 491
BseNI ACTGG 4 cut(s) 179, 378, 439, 595
BseRI GAGGAG 3 cut(s) 139, 242, 348
BseXI GCAGC 3 cut(s) 142, 211, 325
BseYI CCCAGC 1 cut(s) 160
Bsh1236I CGCG 1 cut(s) 129
BsiSI CCGG 2 cut(s) 18, 234
BslFI GGGAC 2 cut(s) 78, 555
BslI CCNNNNNNNGG 2 cut(s) 332, 491
BsmAI GTCTC 2 cut(s) 105, 254
BsmFI GGGAC 2 cut(s) 78, 555
Bso31I GGTCTC 1 cut(s) 254
Bsp143I GATC 3 cut(s) 285, 429, 548
BspACI CCGC 6 cut(s) 15, 33, 54, 120, 127, 197
BspFNI CGCG 1 cut(s) 129
BspPI GGATC 1 cut(s) 543
BspTNI GGTCTC 1 cut(s) 254
BsrI ACTGG 4 cut(s) 179, 378, 439, 595
BssMI GATC 3 cut(s) 285, 429, 548
Bst2UI CCWGG 1 cut(s) 519
Bst4CI ACNGT 2 cut(s) 92, 390
Bst6I CTCTTC 2 cut(s) 80, 517
BstBAI YACGTR 1 cut(s) 283
BstF5I GGATG 1 cut(s) 123
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 3 cut(s) 288, 432, 551
BstMAI GTCTC 2 cut(s) 105, 254
BstMBI GATC 3 cut(s) 285, 429, 548
BstMWI GCNNNNNNNGC 3 cut(s) 11, 128, 161
BstNI CCWGG 1 cut(s) 519
BstSCI CCNGG 1 cut(s) 517
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 3 cut(s) 142, 211, 325
BstX2I RGATCY 1 cut(s) 548
BstYI RGATCY 1 cut(s) 548
BtsCI GGATG 1 cut(s) 123
BtsIMutI CAGTG 2 cut(s) 395, 588
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 356
Csp6I GTAC 2 cut(s) 405, 424
CviAII CATG 2 cut(s) 291, 544
CviJI RGCY 8 cut(s) 14, 30, 87, 164, 176, 202, 338, 398
CviKI_1 RGCY 8 cut(s) 14, 30, 87, 164, 176, 202, 338, 398
CviQI GTAC 2 cut(s) 405, 424
DpnI GATC 3 cut(s) 287, 431, 550
DpnII GATC 3 cut(s) 285, 429, 548
Eam1104I CTCTTC 2 cut(s) 80, 517
EarI CTCTTC 2 cut(s) 80, 517
EciI GGCGGA 1 cut(s) 43
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 356
Eco57I CTGAAG 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 517
FaeI CATG 2 cut(s) 294, 547
FaiI YATR 4 cut(s) 38, 292, 545, 606
FaqI GGGAC 2 cut(s) 78, 555
FatI CATG 2 cut(s) 290, 543
FauI CCCGC 2 cut(s) 40, 204
FbaI TGATCA 1 cut(s) 285
Fnu4HI GCNGC 4 cut(s) 15, 156, 200, 339
FokI GGATG 1 cut(s) 110
Fsp4HI GCNGC 4 cut(s) 15, 156, 200, 339
FspBI CTAG 1 cut(s) 203
GlaI GCGC 1 cut(s) 130
GluI GCNGC 4 cut(s) 15, 156, 200, 339
GsaI CCCAGC 1 cut(s) 164
GsuI CTGGAG 1 cut(s) 162
HapII CCGG 2 cut(s) 18, 234
HhaI GCGC 1 cut(s) 131
Hin1II CATG 2 cut(s) 294, 547
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HinfI GANTC 5 cut(s) 21, 143, 186, 361, 514
HpaII CCGG 2 cut(s) 18, 234
HphI GGTGA 4 cut(s) 103, 213, 291, 473
Hpy188III TCNNGA 2 cut(s) 61, 503
HpyAV CCTTC 3 cut(s) 118, 347, 521
HpyCH4III ACNGT 2 cut(s) 92, 390
HpyCH4IV ACGT 1 cut(s) 282
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 128, 161
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 2 cut(s) 294, 547
HspAI GCGC 1 cut(s) 129
Ksp22I TGATCA 1 cut(s) 285
Kzo9I GATC 3 cut(s) 285, 429, 548
LmnI GCTCC 2 cut(s) 181, 335
Lsp1109I GCAGC 3 cut(s) 142, 211, 325
LweI GCATC 1 cut(s) 312
MaeI CTAG 1 cut(s) 203
MaeII ACGT 1 cut(s) 282
MalI GATC 3 cut(s) 287, 431, 550
MboI GATC 3 cut(s) 285, 429, 548
MboII GAAGA 5 cut(s) 16, 67, 220, 470, 534
MflI RGATCY 1 cut(s) 548
MluCI AATT 2 cut(s) 275, 584
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 2 cut(s) 163, 514
MseI TTAA 1 cut(s) 582
MspI CCGG 2 cut(s) 18, 234
MspR9I CCNGG 1 cut(s) 519
MvaI CCWGG 1 cut(s) 519
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 3 cut(s) 11, 128, 161
NdeII GATC 3 cut(s) 285, 429, 548
NlaIII CATG 2 cut(s) 294, 547
NmeAIII GCCGAG 1 cut(s) 157
PfeI GAWTC 4 cut(s) 21, 186, 361, 514
PfoI TCCNGGA 1 cut(s) 517
PkrI GCNGC 4 cut(s) 16, 157, 201, 340
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
Ppu21I YACGTR 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 517
PspFI CCCAGC 1 cut(s) 160
PspGI CCWGG 1 cut(s) 517
PspPI GGNCC 1 cut(s) 356
PsuI RGATCY 1 cut(s) 548
RsaI GTAC 2 cut(s) 406, 425
RsaNI GTAC 2 cut(s) 405, 424
SaqAI TTAA 1 cut(s) 582
SatI GCNGC 4 cut(s) 15, 156, 200, 339
Sau3AI GATC 3 cut(s) 285, 429, 548
Sau96I GGNCC 1 cut(s) 356
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 1 cut(s) 519
SfaNI GCATC 1 cut(s) 312
SinI GGWCC 1 cut(s) 356
SmlI CTYRAG 1 cut(s) 9
SmoI CTYRAG 1 cut(s) 9
Sse9I AATT 2 cut(s) 275, 584
SsiI CCGC 6 cut(s) 15, 33, 54, 120, 127, 197
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 517
TaaI ACNGT 2 cut(s) 92, 390
TaiI ACGT 1 cut(s) 285
TaqII GACCGA 1 cut(s) 373
TasI AATT 2 cut(s) 275, 584
TatI WGTACW 1 cut(s) 423
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 4 cut(s) 21, 186, 361, 514
Tru1I TTAA 1 cut(s) 582
Tru9I TTAA 1 cut(s) 582
TscAI CASTG 2 cut(s) 395, 595
TseI GCWGC 3 cut(s) 155, 199, 338
TspGWI ACGGA 2 cut(s) 245, 477
TspRI CASTG 2 cut(s) 395, 595
VpaK11BI GGWCC 1 cut(s) 356
XapI RAATTY 2 cut(s) 275, 584
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.