MD13G1191300.v1.1

Photosystem I reaction center subunit II

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014442
Physical Location & Seq
Forward (+)
16389984 .. 16390937
954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1191300.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGCCATGGCAACACAAGCCAGCCTCTTCACCCCAACTCCCTCAGCCCCAAAGACCACTGCTGACCGTACAACCACCCCATGGAAGCAATCAGTCTCCTCCTCCTTCATGGCCCCCAAGCCACTCAAGCTCTCCACCGCAAGAACGATGAGGATCAATGCCTCCGCTGAGGAAAAAACCGTGACCCCCACAAAGGAGGCCCCGGTAGGCTTCACCCCACCCGAGCTGGACCCAACAACACCCTCACCGATCTTCGGTGGCAGCACTGGCGGGCTGTTGAGGAAAGCGCAGGAGGAAGAGTTTTACGTGATCACATGGGAGTCACCAAAGGAGCAGATATTTGAGATGCCGACTGGCGGAGCTGCCATCATGAGGGAGGGTCCTAACTTGCTGAAACTGGCCAGGAAAGAGCAGTGCTTGGCTCTTGGAACTAGGCTTAGGTCCAAGTACAAGATTAAGTACCAGTTTTACAGGGTTTTCCCTAACGGGGAGGTCCAATACTTGCACCCCAAGGATGGTGTGTACCCTGAGAAGGTGAACCCTGGGCGCCAAGGGGTTGGCCAGAACTTCAGGTCGATTGGGAAGAATGTCAGCCCGATTGAGGTCAAGTTCACCGGCAAGCAAGTCTATGACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PsaD

Protein Analysis

213

Amino Acids

23.46

Weight (kDa)

9.68

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaD PF02531 80 - 210 1.8e-68 PsaD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014442)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03130 AT4G02770
fragaria_vesca FvH4_4g15260
malus_domestica MD13G1191300.v1.1 MD16G1191400.v1.1
prunus_persica Prupe.1G011500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0416571
rosa_laevigata RLG00000007973
rosa_multiflora Rmu_sc0003722.1_g000040
rosa_roxburghii Rroxscaffold_5G00360450
rosa_rugosa Rorug04G0143000
rosa_samantha Rh4AG205400 Rh4BG203000 Rh4CG216300 Rh4DG202500
rosa_wichuraiana Rw0G013510 Rw4G017390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 546
AccB7I CCANNNNNTGG 1 cut(s) 81
AciI CCGC 4 cut(s) 138, 165, 270, 357
AclWI GGATC 1 cut(s) 161
AcoI YGGCCR 3 cut(s) 3, 399, 559
AcuI CTGAAG 1 cut(s) 553
AcyI GRCGYC 1 cut(s) 547
AfaI GTAC 4 cut(s) 70, 449, 461, 524
AfiI CCNNNNNNNGG 9 cut(s) 81, 193, 254, 356, 372, 487, 515, 532, 601
AjnI CCWGG 2 cut(s) 401, 541
AluBI AGCT 3 cut(s) 130, 226, 362
AluI AGCT 3 cut(s) 130, 226, 362
Alw26I GTCTC 1 cut(s) 100
AlwI GGATC 1 cut(s) 161
Ama87I CYCGRG 1 cut(s) 221
AoxI GGCC 5 cut(s) 3, 111, 198, 399, 559
ApeKI GCWGC 2 cut(s) 261, 362
AspLEI GCGC 2 cut(s) 289, 549
AspS9I GGNCC 6 cut(s) 112, 199, 229, 380, 441, 493
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 6 cut(s) 22, 205, 237, 315, 547, 604
AvaI CYCGRG 1 cut(s) 221
AvaII GGWCC 4 cut(s) 229, 380, 441, 493
BalI TGGCCA 3 cut(s) 5, 401, 561
BanI GGYRCC 1 cut(s) 546
BbvCI CCTCAGC 2 cut(s) 43, 168
BbvI GCAGC 2 cut(s) 273, 349
BccI CCATC 2 cut(s) 374, 509
BciT130I CCWGG 2 cut(s) 403, 543
BclI TGATCA 1 cut(s) 309
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 100
BfaI CTAG 1 cut(s) 432
BfoI RGCGCY 1 cut(s) 550
BisI GCNGC 2 cut(s) 262, 363
BlsI GCNGC 2 cut(s) 263, 364
Bme1390I CCNGG 3 cut(s) 203, 403, 543
Bme18I GGWCC 4 cut(s) 229, 380, 441, 493
BmeT110I CYCGRG 1 cut(s) 221
BmgT120I GGNCC 6 cut(s) 112, 199, 229, 380, 441, 493
BmiI GGNNCC 5 cut(s) 114, 201, 231, 381, 548
BmrFI CCNGG 3 cut(s) 203, 403, 543
BmsI GCATC 1 cut(s) 336
Bpu10I CCTNAGC 3 cut(s) 43, 168, 437
BpuEI CTTGAG 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 203
BsaAI YACGTR 1 cut(s) 307
BsaBI GATNNNNATC 1 cut(s) 152
BsaHI GRCGYC 1 cut(s) 547
BsaJI CCNNGG 7 cut(s) 6, 80, 201, 510, 541, 542, 550
BsaXI ACNNNNNCTCC 4 cut(s) 22, 52, 284, 314
Bsc4I CCNNNNNNNGG 9 cut(s) 81, 193, 254, 356, 372, 487, 515, 532, 601
Bse118I RCCGGY 1 cut(s) 614
Bse1I ACTGG 4 cut(s) 271, 358, 402, 463
Bse8I GATNNNNATC 1 cut(s) 152
BseBI CCWGG 2 cut(s) 403, 543
BseDI CCNNGG 7 cut(s) 6, 80, 201, 510, 541, 542, 550
BseGI GGATG 1 cut(s) 520
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 9 cut(s) 81, 193, 254, 356, 372, 487, 515, 532, 601
BseMII CTCAG 3 cut(s) 57, 159, 519
BseNI ACTGG 4 cut(s) 271, 358, 402, 463
BseRI GAGGAG 2 cut(s) 88, 91
BseXI GCAGC 2 cut(s) 273, 349
BshFI GGCC 5 cut(s) 5, 113, 200, 401, 561
BshNI GGYRCC 1 cut(s) 546
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 2 cut(s) 203, 615
BslI CCNNNNNNNGG 9 cut(s) 81, 193, 254, 356, 372, 487, 515, 532, 601
BsmAI GTCTC 1 cut(s) 100
BsnI GGCC 5 cut(s) 5, 113, 200, 401, 561
BsoBI CYCGRG 1 cut(s) 221
Bsp143I GATC 3 cut(s) 153, 249, 309
Bsp19I CCATGG 2 cut(s) 6, 80
BspACI CCGC 4 cut(s) 138, 165, 270, 357
BspANI GGCC 5 cut(s) 5, 113, 200, 401, 561
BspCNI CTCAG 3 cut(s) 56, 160, 520
BspHI TCATGA 1 cut(s) 369
BspLI GGNNCC 5 cut(s) 114, 201, 231, 381, 548
BspPI GGATC 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 546
BsrFI RCCGGY 1 cut(s) 614
BsrI ACTGG 4 cut(s) 271, 358, 402, 463
BssAI RCCGGY 1 cut(s) 614
BssECI CCNNGG 7 cut(s) 6, 80, 201, 510, 541, 542, 550
BssMI GATC 3 cut(s) 153, 249, 309
BssNI GRCGYC 1 cut(s) 547
BssT1I CCWWGG 4 cut(s) 6, 80, 510, 550
Bst2UI CCWGG 2 cut(s) 403, 543
Bst4CI ACNGT 2 cut(s) 68, 181
Bst6I CTCTTC 2 cut(s) 32, 291
BstACI GRCGYC 1 cut(s) 547
BstBAI YACGTR 1 cut(s) 307
BstC8I GCNNGC 3 cut(s) 22, 272, 620
BstDEI CTNAG 4 cut(s) 43, 168, 437, 528
BstDSI CCRYGG 2 cut(s) 6, 80
BstF5I GGATG 1 cut(s) 520
BstH2I RGCGCY 1 cut(s) 550
BstHHI GCGC 2 cut(s) 289, 549
BstKTI GATC 3 cut(s) 156, 252, 312
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 3 cut(s) 153, 249, 309
BstMWI GCNNNNNNNGC 3 cut(s) 17, 127, 267
BstNI CCWGG 2 cut(s) 403, 543
BstSCI CCNGG 3 cut(s) 201, 401, 541
BstV1I GCAGC 2 cut(s) 273, 349
BstXI CCANNNNNNTGG 1 cut(s) 557
BsuRI GGCC 5 cut(s) 5, 113, 200, 401, 561
BtgI CCRYGG 2 cut(s) 6, 80
BtsCI GGATG 1 cut(s) 520
BtsI GCAGTG 2 cut(s) 57, 419
BtsIMutI CAGTG 3 cut(s) 57, 264, 419
Cac8I GCNNGC 3 cut(s) 22, 272, 620
CciI TCATGA 1 cut(s) 369
CfoI GCGC 2 cut(s) 289, 549
Cfr10I RCCGGY 1 cut(s) 614
Cfr13I GGNCC 6 cut(s) 112, 199, 229, 380, 441, 493
Csp6I GTAC 4 cut(s) 69, 448, 460, 523
CviAII CATG 5 cut(s) 7, 81, 109, 315, 370
CviQI GTAC 4 cut(s) 69, 448, 460, 523
DdeI CTNAG 4 cut(s) 43, 168, 437, 528
DinI GGCGCC 1 cut(s) 548
DpnI GATC 3 cut(s) 155, 251, 311
DpnII GATC 3 cut(s) 153, 249, 309
EaeI YGGCCR 3 cut(s) 3, 399, 559
Eam1104I CTCTTC 2 cut(s) 32, 291
EarI CTCTTC 2 cut(s) 32, 291
EciI GGCGGA 1 cut(s) 372
Eco130I CCWWGG 4 cut(s) 6, 80, 510, 550
Eco47I GGWCC 4 cut(s) 229, 380, 441, 493
Eco57I CTGAAG 1 cut(s) 553
Eco88I CYCGRG 1 cut(s) 221
EcoO109I RGGNCCY 2 cut(s) 199, 380
EcoRII CCWGG 2 cut(s) 401, 541
EcoT14I CCWWGG 4 cut(s) 6, 80, 510, 550
EgeI GGCGCC 1 cut(s) 548
EheI GGCGCC 1 cut(s) 548
ErhI CCWWGG 4 cut(s) 6, 80, 510, 550
FaeI CATG 5 cut(s) 10, 84, 112, 318, 373
FaiI YATR 8 cut(s) 8, 82, 110, 316, 371, 630, 635, 637
FatI CATG 5 cut(s) 6, 80, 108, 314, 369
FauI CCCGC 1 cut(s) 263
FauNDI CATATG 1 cut(s) 635
FbaI TGATCA 1 cut(s) 309
Fnu4HI GCNGC 2 cut(s) 262, 363
FokI GGATG 1 cut(s) 527
Fsp4HI GCNGC 2 cut(s) 262, 363
FspBI CTAG 1 cut(s) 432
GlaI GCGC 2 cut(s) 288, 548
GluI GCNGC 2 cut(s) 262, 363
HaeII RGCGCY 1 cut(s) 550
HaeIII GGCC 5 cut(s) 5, 113, 200, 401, 561
HapII CCGG 2 cut(s) 203, 615
HhaI GCGC 2 cut(s) 289, 549
Hin1I GRCGYC 1 cut(s) 547
Hin1II CATG 5 cut(s) 10, 84, 112, 318, 373
Hin6I GCGC 2 cut(s) 287, 547
HinP1I GCGC 2 cut(s) 287, 547
HinfI GANTC 1 cut(s) 320
HpaII CCGG 2 cut(s) 203, 615
HphI GGTGA 6 cut(s) 22, 205, 237, 315, 547, 604
Hpy166II GTNNAC 3 cut(s) 523, 538, 612
Hpy188III TCNNGA 1 cut(s) 370
Hpy8I GTNNAC 3 cut(s) 523, 538, 612
HpyAV CCTTC 2 cut(s) 115, 526
HpyCH4III ACNGT 2 cut(s) 68, 181
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 1 cut(s) 505
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 127, 267
HpyF3I CTNAG 4 cut(s) 43, 168, 437, 528
HpySE526I ACGT 1 cut(s) 306
Hsp92I GRCGYC 1 cut(s) 547
Hsp92II CATG 5 cut(s) 10, 84, 112, 318, 373
HspAI GCGC 2 cut(s) 287, 547
KasI GGCGCC 1 cut(s) 546
Ksp22I TGATCA 1 cut(s) 309
Kzo9I GATC 3 cut(s) 153, 249, 309
LmnI GCTCC 2 cut(s) 331, 359
Lsp1109I GCAGC 2 cut(s) 273, 349
LweI GCATC 1 cut(s) 336
MaeI CTAG 1 cut(s) 432
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 2 cut(s) 181, 321
MalI GATC 3 cut(s) 155, 251, 311
MboI GATC 3 cut(s) 153, 249, 309
MboII GAAGA 4 cut(s) 19, 244, 308, 595
MlsI TGGCCA 3 cut(s) 5, 401, 561
MluNI TGGCCA 3 cut(s) 5, 401, 561
Mly113I GGCGCC 1 cut(s) 547
MlyI GAGTC 1 cut(s) 329
Mox20I TGGCCA 3 cut(s) 5, 401, 561
MscI TGGCCA 3 cut(s) 5, 401, 561
MseI TTAA 1 cut(s) 456
Msp20I TGGCCA 3 cut(s) 5, 401, 561
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 2 cut(s) 203, 615
MspR9I CCNGG 3 cut(s) 203, 403, 543
MvaI CCWGG 2 cut(s) 403, 543
MwoI GCNNNNNNNGC 3 cut(s) 17, 127, 267
NarI GGCGCC 1 cut(s) 547
NciI CCSGG 1 cut(s) 203
NcoI CCATGG 2 cut(s) 6, 80
NdeI CATATG 1 cut(s) 635
NdeII GATC 3 cut(s) 153, 249, 309
NlaIII CATG 5 cut(s) 10, 84, 112, 318, 373
NlaIV GGNNCC 5 cut(s) 114, 201, 231, 381, 548
NmuCI GTSAC 2 cut(s) 181, 321
PagI TCATGA 1 cut(s) 369
PasI CCCWGGG 1 cut(s) 542
PflMI CCANNNNNTGG 1 cut(s) 81
PkrI GCNGC 2 cut(s) 263, 364
PleI GAGTC 1 cut(s) 328
PluTI GGCGCC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 328
Ppu21I YACGTR 1 cut(s) 307
PpuMI RGGWCCY 1 cut(s) 380
Psp5II RGGWCCY 1 cut(s) 380
Psp6I CCWGG 2 cut(s) 401, 541
PspGI CCWGG 2 cut(s) 401, 541
PspN4I GGNNCC 5 cut(s) 114, 201, 231, 381, 548
PspPI GGNCC 6 cut(s) 112, 199, 229, 380, 441, 493
PspPPI RGGWCCY 1 cut(s) 380
RsaI GTAC 4 cut(s) 70, 449, 461, 524
RsaNI GTAC 4 cut(s) 69, 448, 460, 523
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 2 cut(s) 262, 363
Sau3AI GATC 3 cut(s) 153, 249, 309
Sau96I GGNCC 6 cut(s) 112, 199, 229, 380, 441, 493
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 3 cut(s) 203, 403, 543
SetI ASST 9 cut(s) 132, 228, 309, 364, 443, 495, 537, 575, 606
SfaNI GCATC 1 cut(s) 336
SfoI GGCGCC 1 cut(s) 548
SinI GGWCC 4 cut(s) 229, 380, 441, 493
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SsiI CCGC 4 cut(s) 138, 165, 270, 357
SspDI GGCGCC 1 cut(s) 546
SspMI CTAG 1 cut(s) 432
StyD4I CCNGG 3 cut(s) 201, 401, 541
StyI CCWWGG 4 cut(s) 6, 80, 510, 550
TaaI ACNGT 2 cut(s) 68, 181
TaiI ACGT 1 cut(s) 309
TaqI TCGA 1 cut(s) 575
TatI WGTACW 1 cut(s) 447
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TscAI CASTG 3 cut(s) 64, 271, 419
TseFI GTSAC 2 cut(s) 181, 321
TseI GCWGC 2 cut(s) 261, 362
Tsp45I GTSAC 2 cut(s) 181, 321
TspDTI ATGAA 1 cut(s) 97
TspRI CASTG 3 cut(s) 64, 271, 419
Van91I CCANNNNNTGG 1 cut(s) 81
VpaK11BI GGWCC 4 cut(s) 229, 380, 441, 493
XspI CTAG 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.