Rw0G013510

Photosystem I reaction center subunit II

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00608
OG0014442
Physical Location & Seq
Reverse (-)
8306 .. 8848
543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G013510.1

Sequence Viewer

Length: 543 bp
ATGGCCATGGCAACTCAAGCGACCCTCTTCACCCCAACCGCCTCAGCACCATGGAAACAGTCAGTCACCTCCACCTTCATGGCCCCAAAGTCACTGACCCTCTCCACCGCAGCGCCAAGACCCATGAGGATCGCAGCTGCTGAAGAGAAAACCGTGGCACCCACAAAGGAGGCCGAAGCTCCGGTTGGCTTCACCCCGCCGGAACTGGACCCAACAACCCCATCCCCAATCTTCGGCGGCAGCACCGGTGGGCTTCTCCGCAAGGCCCAAGAGGAAGAGTTCTACGTGATCACCTGGGAATCACCCAAGGAGCAAATCTTTGAGATGCCAACTGGTGGTGCAGCCATCATGAGGGAAGGACCTAACTTGTTGAAGCTGGCCAGGAAAGAGCAGTGTTTGGCTCTTGGGACTAGACTGAGGTCCAAGTACAAGATCAAGTACCAGTTTTACAGGGTGTTCCCTAATGGTGAGGTTCAGTATTTGCACCCAAAAGATGGAGTGTACCCTGAGAAGGTGAACCCGGGCGCCAAGGAGTGGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PsaD

Protein Analysis

180

Amino Acids

19.79

Weight (kDa)

9.14

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaD PF02531 73 - 177 1.2e-56 PsaD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014442)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03130 AT4G02770
fragaria_vesca FvH4_4g15260
malus_domestica MD13G1191300.v1.1 MD16G1191400.v1.1
prunus_persica Prupe.1G011500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0416571
rosa_laevigata RLG00000007973
rosa_multiflora Rmu_sc0003722.1_g000040
rosa_roxburghii Rroxscaffold_5G00360450
rosa_rugosa Rorug04G0143000
rosa_samantha Rh4AG205400 Rh4BG203000 Rh4CG216300 Rh4DG202500
rosa_wichuraiana Rw0G013510 Rw4G017390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 157, 524
AccB7I CCANNNNNTGG 3 cut(s) 335, 494, 534
AciI CCGC 5 cut(s) 39, 108, 197, 237, 259
AclWI GGATC 1 cut(s) 137
AcoI YGGCCR 2 cut(s) 3, 378
AcuI CTGAAG 1 cut(s) 162
AcyI GRCGYC 1 cut(s) 525
AfaI GTAC 3 cut(s) 428, 440, 503
AfiI CCNNNNNNNGG 6 cut(s) 233, 335, 351, 494, 511, 534
AgeI ACCGGT 1 cut(s) 245
AgsI TTSAA 1 cut(s) 373
AjnI CCWGG 2 cut(s) 293, 380
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AluBI AGCT 3 cut(s) 137, 179, 376
AluI AGCT 3 cut(s) 137, 179, 376
AlwI GGATC 1 cut(s) 137
AlwNI CAGNNNCTG 1 cut(s) 140
Ama87I CYCGRG 1 cut(s) 520
AoxI GGCC 5 cut(s) 3, 81, 171, 264, 378
ApeKI GCWGC 5 cut(s) 110, 134, 137, 240, 341
AsiGI ACCGGT 1 cut(s) 245
AspLEI GCGC 2 cut(s) 115, 527
AspS9I GGNCC 5 cut(s) 82, 208, 265, 359, 420
AsuC2I CCSGG 2 cut(s) 521, 522
AsuHPI GGTGA 7 cut(s) 22, 58, 184, 283, 294, 479, 526
AvaI CYCGRG 1 cut(s) 520
AvaII GGWCC 3 cut(s) 208, 359, 420
BalI TGGCCA 2 cut(s) 5, 380
BanI GGYRCC 2 cut(s) 157, 524
BbvCI CCTCAGC 1 cut(s) 43
BbvI GCAGC 5 cut(s) 122, 124, 146, 252, 353
BccI CCATC 3 cut(s) 229, 353, 488
BciT130I CCWGG 2 cut(s) 295, 382
BclI TGATCA 1 cut(s) 288
BcnI CCSGG 2 cut(s) 521, 522
BfaI CTAG 1 cut(s) 411
BfoI RGCGCY 2 cut(s) 116, 528
BisI GCNGC 6 cut(s) 111, 135, 138, 238, 241, 342
BlsI GCNGC 6 cut(s) 112, 136, 139, 239, 242, 343
Bme1390I CCNGG 4 cut(s) 295, 382, 521, 522
Bme18I GGWCC 3 cut(s) 208, 359, 420
BmeT110I CYCGRG 1 cut(s) 520
BmgT120I GGNCC 5 cut(s) 82, 208, 265, 359, 420
BmiI GGNNCC 4 cut(s) 84, 159, 210, 526
BmrFI CCNGG 4 cut(s) 295, 382, 521, 522
BmsI GCATC 1 cut(s) 315
BoxI GACNNNNGTC 1 cut(s) 418
Bpu10I CCTNAGC 1 cut(s) 43
BpuMI CCSGG 2 cut(s) 521, 522
BsaAI YACGTR 1 cut(s) 286
BsaHI GRCGYC 1 cut(s) 525
BsaJI CCNNGG 7 cut(s) 6, 50, 153, 294, 306, 520, 528
BsaWI WCCGGW 2 cut(s) 181, 245
Bsc4I CCNNNNNNNGG 6 cut(s) 233, 335, 351, 494, 511, 534
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 3 cut(s) 210, 337, 442
BseBI CCWGG 2 cut(s) 295, 382
BseDI CCNNGG 7 cut(s) 6, 50, 153, 294, 306, 520, 528
BseGI GGATG 1 cut(s) 221
BseLI CCNNNNNNNGG 6 cut(s) 233, 335, 351, 494, 511, 534
BseMII CTCAG 3 cut(s) 57, 407, 498
BseNI ACTGG 3 cut(s) 210, 337, 442
BseXI GCAGC 5 cut(s) 122, 124, 146, 252, 353
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 5 cut(s) 5, 83, 173, 266, 380
BshNI GGYRCC 2 cut(s) 157, 524
BshTI ACCGGT 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 520
BsiSI CCGG 4 cut(s) 182, 200, 246, 521
BslFI GGGAC 1 cut(s) 421
BslI CCNNNNNNNGG 6 cut(s) 233, 335, 351, 494, 511, 534
BsmFI GGGAC 1 cut(s) 421
BsnI GGCC 5 cut(s) 5, 83, 173, 266, 380
BsoBI CYCGRG 1 cut(s) 520
Bsp143I GATC 3 cut(s) 129, 288, 432
Bsp19I CCATGG 2 cut(s) 6, 50
BspACI CCGC 5 cut(s) 39, 108, 197, 237, 259
BspANI GGCC 5 cut(s) 5, 83, 173, 266, 380
BspCNI CTCAG 3 cut(s) 56, 408, 499
BspHI TCATGA 1 cut(s) 348
BspLI GGNNCC 4 cut(s) 84, 159, 210, 526
BspPI GGATC 1 cut(s) 137
BspT107I GGYRCC 2 cut(s) 157, 524
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 3 cut(s) 210, 337, 442
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 7 cut(s) 6, 50, 153, 294, 306, 520, 528
BssMI GATC 3 cut(s) 129, 288, 432
BssNI GRCGYC 1 cut(s) 525
BssT1I CCWWGG 4 cut(s) 6, 50, 306, 528
Bst2UI CCWGG 2 cut(s) 295, 382
Bst4CI ACNGT 2 cut(s) 60, 154
Bst6I CTCTTC 3 cut(s) 32, 138, 270
BstACI GRCGYC 1 cut(s) 525
BstBAI YACGTR 1 cut(s) 286
BstC8I GCNNGC 1 cut(s) 378
BstDEI CTNAG 3 cut(s) 43, 416, 507
BstDSI CCRYGG 3 cut(s) 6, 50, 153
BstF5I GGATG 1 cut(s) 221
BstH2I RGCGCY 2 cut(s) 116, 528
BstHHI GCGC 2 cut(s) 115, 527
BstKTI GATC 3 cut(s) 132, 291, 435
BstMBI GATC 3 cut(s) 129, 288, 432
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 2 cut(s) 295, 382
BstPAI GACNNNNGTC 1 cut(s) 418
BstSCI CCNGG 4 cut(s) 293, 380, 519, 520
BstV1I GCAGC 5 cut(s) 122, 124, 146, 252, 353
BstXI CCANNNNNNTGG 1 cut(s) 79
BsuRI GGCC 5 cut(s) 5, 83, 173, 266, 380
BtgI CCRYGG 3 cut(s) 6, 50, 153
BtsCI GGATG 1 cut(s) 221
BtsI GCAGTG 1 cut(s) 398
BtsIMutI CAGTG 2 cut(s) 92, 398
Cac8I GCNNGC 1 cut(s) 378
CaiI CAGNNNCTG 1 cut(s) 140
CciI TCATGA 1 cut(s) 348
CfoI GCGC 2 cut(s) 115, 527
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 5 cut(s) 82, 208, 265, 359, 420
Cfr9I CCCGGG 1 cut(s) 520
Csp6I GTAC 3 cut(s) 427, 439, 502
CspAI ACCGGT 1 cut(s) 245
CviAII CATG 5 cut(s) 7, 51, 79, 124, 349
CviQI GTAC 3 cut(s) 427, 439, 502
DdeI CTNAG 3 cut(s) 43, 416, 507
DinI GGCGCC 1 cut(s) 526
DpnI GATC 3 cut(s) 131, 290, 434
DpnII GATC 3 cut(s) 129, 288, 432
EaeI YGGCCR 2 cut(s) 3, 378
Eam1104I CTCTTC 3 cut(s) 32, 138, 270
EarI CTCTTC 3 cut(s) 32, 138, 270
Eco130I CCWWGG 4 cut(s) 6, 50, 306, 528
Eco47I GGWCC 3 cut(s) 208, 359, 420
Eco57I CTGAAG 1 cut(s) 162
Eco88I CYCGRG 1 cut(s) 520
EcoO109I RGGNCCY 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 293, 380
EcoT14I CCWWGG 4 cut(s) 6, 50, 306, 528
EgeI GGCGCC 1 cut(s) 526
EheI GGCGCC 1 cut(s) 526
ErhI CCWWGG 4 cut(s) 6, 50, 306, 528
FaeI CATG 5 cut(s) 10, 54, 82, 127, 352
FaiI YATR 5 cut(s) 8, 52, 80, 125, 350
FaqI GGGAC 1 cut(s) 421
FatI CATG 5 cut(s) 6, 50, 78, 123, 348
FauI CCCGC 1 cut(s) 204
FbaI TGATCA 1 cut(s) 288
Fnu4HI GCNGC 6 cut(s) 111, 135, 138, 238, 241, 342
FokI GGATG 1 cut(s) 208
Fsp4HI GCNGC 6 cut(s) 111, 135, 138, 238, 241, 342
FspBI CTAG 1 cut(s) 411
GlaI GCGC 2 cut(s) 114, 526
GluI GCNGC 6 cut(s) 111, 135, 138, 238, 241, 342
HaeII RGCGCY 2 cut(s) 116, 528
HaeIII GGCC 5 cut(s) 5, 83, 173, 266, 380
HapII CCGG 4 cut(s) 182, 200, 246, 521
HhaI GCGC 2 cut(s) 115, 527
Hin1I GRCGYC 1 cut(s) 525
Hin1II CATG 5 cut(s) 10, 54, 82, 127, 352
Hin6I GCGC 2 cut(s) 113, 525
HinP1I GCGC 2 cut(s) 113, 525
HinfI GANTC 1 cut(s) 299
HpaII CCGG 4 cut(s) 182, 200, 246, 521
HphI GGTGA 7 cut(s) 22, 58, 184, 283, 294, 479, 526
Hpy166II GTNNAC 2 cut(s) 502, 517
Hpy188III TCNNGA 1 cut(s) 349
Hpy8I GTNNAC 2 cut(s) 502, 517
HpyAV CCTTC 3 cut(s) 85, 350, 505
HpyCH4III ACNGT 2 cut(s) 60, 154
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 2 cut(s) 341, 484
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpyF3I CTNAG 3 cut(s) 43, 416, 507
HpySE526I ACGT 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 525
Hsp92II CATG 5 cut(s) 10, 54, 82, 127, 352
HspAI GCGC 2 cut(s) 113, 525
KasI GGCGCC 1 cut(s) 524
Ksp22I TGATCA 1 cut(s) 288
Kzo9I GATC 3 cut(s) 129, 288, 432
LmnI GCTCC 2 cut(s) 184, 310
Lsp1109I GCAGC 5 cut(s) 122, 124, 146, 252, 353
LweI GCATC 1 cut(s) 315
MaeI CTAG 1 cut(s) 411
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 2 cut(s) 64, 90
MalI GATC 3 cut(s) 131, 290, 434
MboI GATC 3 cut(s) 129, 288, 432
MboII GAAGA 4 cut(s) 19, 155, 223, 287
MlsI TGGCCA 2 cut(s) 5, 380
MluNI TGGCCA 2 cut(s) 5, 380
Mly113I GGCGCC 1 cut(s) 525
Mox20I TGGCCA 2 cut(s) 5, 380
MscI TGGCCA 2 cut(s) 5, 380
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 2 cut(s) 5, 380
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 4 cut(s) 182, 200, 246, 521
MspR9I CCNGG 4 cut(s) 295, 382, 521, 522
MvaI CCWGG 2 cut(s) 295, 382
MwoI GCNNNNNNNGC 1 cut(s) 17
NarI GGCGCC 1 cut(s) 525
NciI CCSGG 2 cut(s) 521, 522
NcoI CCATGG 2 cut(s) 6, 50
NdeII GATC 3 cut(s) 129, 288, 432
NlaIII CATG 5 cut(s) 10, 54, 82, 127, 352
NlaIV GGNNCC 4 cut(s) 84, 159, 210, 526
NmuCI GTSAC 2 cut(s) 64, 90
PagI TCATGA 1 cut(s) 348
PfeI GAWTC 1 cut(s) 299
PflMI CCANNNNNTGG 3 cut(s) 335, 494, 534
PinAI ACCGGT 1 cut(s) 245
PkrI GCNGC 6 cut(s) 112, 136, 139, 239, 242, 343
PluTI GGCGCC 1 cut(s) 528
Ppu21I YACGTR 1 cut(s) 286
PpuMI RGGWCCY 1 cut(s) 359
PshAI GACNNNNGTC 1 cut(s) 418
Psp5II RGGWCCY 1 cut(s) 359
Psp6I CCWGG 2 cut(s) 293, 380
PspGI CCWGG 2 cut(s) 293, 380
PspN4I GGNNCC 4 cut(s) 84, 159, 210, 526
PspPI GGNCC 5 cut(s) 82, 208, 265, 359, 420
PspPPI RGGWCCY 1 cut(s) 359
PstNI CAGNNNCTG 1 cut(s) 140
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 3 cut(s) 428, 440, 503
RsaNI GTAC 3 cut(s) 427, 439, 502
RseI CAYNNNNRTG 1 cut(s) 77
SatI GCNGC 6 cut(s) 111, 135, 138, 238, 241, 342
Sau3AI GATC 3 cut(s) 129, 288, 432
Sau96I GGNCC 5 cut(s) 82, 208, 265, 359, 420
ScrFI CCNGG 4 cut(s) 295, 382, 521, 522
SfaNI GCATC 1 cut(s) 315
SfoI GGCGCC 1 cut(s) 526
SgrAI CRCCGGYG 1 cut(s) 245
SinI GGWCC 3 cut(s) 208, 359, 420
SmaI CCCGGG 1 cut(s) 522
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 1 cut(s) 15
SmoI CTYRAG 1 cut(s) 15
SsiI CCGC 5 cut(s) 39, 108, 197, 237, 259
SspDI GGCGCC 1 cut(s) 524
SspMI CTAG 1 cut(s) 411
StyD4I CCNGG 4 cut(s) 293, 380, 519, 520
StyI CCWWGG 4 cut(s) 6, 50, 306, 528
TaaI ACNGT 2 cut(s) 60, 154
TaiI ACGT 1 cut(s) 288
TatI WGTACW 1 cut(s) 426
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 1 cut(s) 299
TscAI CASTG 2 cut(s) 99, 398
TseFI GTSAC 2 cut(s) 64, 90
TseI GCWGC 5 cut(s) 110, 134, 137, 240, 341
Tsp45I GTSAC 2 cut(s) 64, 90
TspDTI ATGAA 1 cut(s) 67
TspMI CCCGGG 1 cut(s) 520
TspRI CASTG 2 cut(s) 99, 398
Van91I CCANNNNNTGG 3 cut(s) 335, 494, 534
VpaK11BI GGWCC 3 cut(s) 208, 359, 420
XmaI CCCGGG 1 cut(s) 520
XspI CTAG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.