AT1G04280

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007045
Physical Location & Seq
Reverse (-)
1143436 .. 1147091
3656 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G04280.2

Sequence Viewer

Length: 1605 bp
ATGGTGAAACCCTTAGGAGAAGGAACAAGAGATGTCTCTAATGTTGGTTGTAAATCAGTTGATCTCCTCCTAGCTTCTCTTGGTGGTCTTGCCGCCGCTGTAGCAGCTGCTTACGCCGGAGAATTACTCCTTCGACGCCGGAAACTTGATCAAGGTGCTTCCATGGGGTATAAAGATGTCAAAATCGCCCCTTTGATTGAGAGGAAAGACTCAGGTCGACGATCAAACCTAGAGAGATTCTCCCACTATGTTGCAAGGCAGCTCGGATTTGAGGATCCAAATGAATACCCACAACTATGCAAATTAGCAAATGGATATTTGTTGAAAACAAAAGGGTATGATGAGAATGTGGATGAGTATTTGGAGAATGAAGCTGAGCGTGATTCTCTCTATGTTCATCTCCTTGAAGAATTTGATAGATGCATTCTCACTTATTTTTCTTTCAATTGGACCCAATCTTCCAACCTCATCTCTCAGGCTTTAAGCGATGAGTCGGATCAGAAAGTTCCAAAACTCAAGGATTTCGTGATGGCAGCAACAAGGAAACAGAGGTTTGAGAGAGTAACAAAAGATCTCAAGGTGAAAAGGGTAATTTCGACATTAGTAGAAGAGATGAGAGTCATTGGGAGCGGAAGCAGTGAGCCACACTGTACGGAAGTAATGTCTCCGGTGGCTCATAACAAACGAAGTCCCGTCCTTCTCCTTATGGGCGGAGGAATGGGCGCAGGAAAGAGCACCGTCCTCAAAGATATCTTCCTAGAGTCGTTTTGGTCGGAGGCACAGGCCGACGCGGTGGTTATAGAGGCAGACGCGTTTAAGGAGACGGATGTAATTTATAGAGCTCTTAGCTCTAGAGGTCACCACGATGATATGCTTCAAACTGCTGAATTGGTTCATCAATCATCAACGGATGCTGCATCGTCCTTACTAGTAACAGCATTGAACGACGGACGTGATGTGATAATGGATGGAACATTGTCATGGGAGCCATTCGTCGAACAAATGATCGAAATGGCAAGAAACGTCCACAAACAAAAATATCGAATGGGCGAAGGCTACAAGGTGTCCGAAGAGGGGACGATAACCGAAAAATATTGGGAAGAAGAAGAAGAAGAAACGAAAGAAAATGGCAAACAACAAAATCTGAAACCTTACAGAATCGAGCTGGTCGGTGTGGTTTGTGACGCTTATCTTGCCGTAGCAAGAGGCATACGGAGAGCTCTAATGGTGAAGAGAGCAGTGAGAGTGAAACCACAATTGAATTCACACAAGAGATTTGCTAATGCATTCCCAAAGTATTGTGAGCTTGTTGATAACGCTAGGCTTTATTGCACCAATGCTGTTGGTGGTCCTCCAAGGCTTATAGCGTGGAAAGACGGAAATAGTAAGCTTCTGGTGGATCCAGAGGATATTGATTGTCTAAAACGGGTCAGCAGTCTCAACCCGGATGCAGAATCCATTTACGAGCTTTATCCGGATCCAAGCCAATTAAGCAAACCCGGTTCTGTTTGGAATGATGTTGTGTTAGTTCCATCGAGGCCCAAGGTCCAGAAGGAACTTAGCGATGCAATCAGGAGAATCGAAAAGGCCCAACCAAAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

534

Amino Acids

59.98

Weight (kDa)

6.11

Isoelectric Point (pI)

46.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 172 - 287 1.6e-13 Zeta toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 630
AccI GTMKAC 1 cut(s) 217
AccII CGCG 2 cut(s) 791, 812
AccIII TCCGGA 1 cut(s) 1474
AciI CCGC 5 cut(s) 93, 96, 630, 711, 791
AclWI GGATC 7 cut(s) 269, 282, 504, 1394, 1407, 1472, 1485
AcsI RAATTY 2 cut(s) 410, 1261
AcyI GRCGYC 1 cut(s) 136
AfaI GTAC 1 cut(s) 652
AfiI CCNNNNNNNGG 1 cut(s) 1074
AflIII ACRYGT 1 cut(s) 810
AgsI TTSAA 6 cut(s) 325, 407, 445, 878, 943, 1261
AhlI ACTAGT 1 cut(s) 928
AjiI CACGTC 1 cut(s) 953
Alw21I GWGCWC 3 cut(s) 737, 844, 1222
Alw26I GTCTC 4 cut(s) 40, 669, 815, 1442
AlwI GGATC 7 cut(s) 269, 282, 504, 1394, 1407, 1472, 1485
Aor13HI TCCGGA 1 cut(s) 1474
AoxI GGCC 3 cut(s) 783, 1538, 1587
ApeKI GCWGC 5 cut(s) 104, 107, 259, 533, 914
ApoI RAATTY 2 cut(s) 410, 1261
Asp700I GAANNNNTTC 1 cut(s) 891
AspLEI GCGC 1 cut(s) 725
AspS9I GGNCC 5 cut(s) 450, 1349, 1539, 1546, 1588
AsuC2I CCSGG 2 cut(s) 1445, 1500
AsuHPI GGTGA 4 cut(s) 16, 592, 851, 1240
AvaII GGWCC 3 cut(s) 450, 1349, 1546
AxyI CCTNAGG 1 cut(s) 13
BamHI GGATCC 3 cut(s) 274, 1399, 1477
BanII GRGCYC 2 cut(s) 844, 1222
Bbv12I GWGCWC 3 cut(s) 737, 844, 1222
BbvI GCAGC 5 cut(s) 94, 116, 271, 545, 901
BccI CCATC 3 cut(s) 523, 962, 1540
BceAI ACGGC 1 cut(s) 1181
BclI TGATCA 1 cut(s) 148
BcnI CCSGG 2 cut(s) 1445, 1500
BcoDI GTCTC 4 cut(s) 40, 669, 815, 1442
BcuI ACTAGT 1 cut(s) 928
BfaI CTAG 6 cut(s) 71, 230, 758, 852, 929, 1320
BfmI CTRYAG 1 cut(s) 99
BglII AGATCT 1 cut(s) 571
BisI GCNGC 7 cut(s) 93, 96, 105, 108, 260, 534, 915
BlpI GCTNAGC 1 cut(s) 375
BlsI GCNGC 7 cut(s) 94, 97, 106, 109, 261, 535, 916
Bme1390I CCNGG 2 cut(s) 1445, 1500
Bme18I GGWCC 3 cut(s) 450, 1349, 1546
BmgBI CACGTC 1 cut(s) 953
BmgT120I GGNCC 5 cut(s) 450, 1349, 1539, 1546, 1588
BmiI GGNNCC 5 cut(s) 276, 452, 987, 1401, 1479
BmrFI CCNGG 2 cut(s) 1445, 1500
BmsI GCATC 5 cut(s) 410, 901, 926, 1438, 1555
BoxI GACNNNNGTC 1 cut(s) 213
BplI GAGNNNNNCTC 4 cut(s) 111, 143, 224, 256
Bpu1102I GCTNAGC 1 cut(s) 375
BpuEI CTTGAG 2 cut(s) 500, 560
BpuMI CCSGG 2 cut(s) 1445, 1500
BsaHI GRCGYC 1 cut(s) 136
BsaJI CCNNGG 3 cut(s) 162, 1355, 1542
BsaWI WCCGGW 2 cut(s) 667, 1474
Bsc4I CCNNNNNNNGG 1 cut(s) 1074
Bse21I CCTNAGG 1 cut(s) 13
BseAI TCCGGA 1 cut(s) 1474
BseDI CCNNGG 3 cut(s) 162, 1355, 1542
BseGI GGATG 5 cut(s) 358, 832, 916, 973, 1453
BseLI CCNNNNNNNGG 1 cut(s) 1074
BseMII CTCAG 3 cut(s) 225, 366, 488
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 5 cut(s) 94, 116, 271, 545, 901
Bsh1236I CGCG 2 cut(s) 791, 812
BshFI GGCC 3 cut(s) 785, 1540, 1589
BsiHKAI GWGCWC 3 cut(s) 737, 844, 1222
BsiSI CCGG 6 cut(s) 117, 139, 668, 1445, 1475, 1500
BslFI GGGAC 2 cut(s) 675, 1090
BslI CCNNNNNNNGG 1 cut(s) 1074
BsmAI GTCTC 4 cut(s) 40, 669, 815, 1442
BsmBI CGTCTC 1 cut(s) 815
BsmFI GGGAC 2 cut(s) 675, 1090
BsmI GAATGC 2 cut(s) 423, 1286
BsnI GGCC 3 cut(s) 785, 1540, 1589
Bsp1286I GDGCHC 3 cut(s) 737, 844, 1222
Bsp13I TCCGGA 1 cut(s) 1474
Bsp143I GATC 9 cut(s) 61, 148, 221, 274, 496, 571, 1005, 1399, 1477
Bsp1720I GCTNAGC 1 cut(s) 375
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 5 cut(s) 93, 96, 630, 711, 791
BspANI GGCC 3 cut(s) 785, 1540, 1589
BspCNI CTCAG 3 cut(s) 224, 367, 487
BspEI TCCGGA 1 cut(s) 1474
BspFNI CGCG 2 cut(s) 791, 812
BspLI GGNNCC 5 cut(s) 276, 452, 987, 1401, 1479
BspPI GGATC 7 cut(s) 269, 282, 504, 1394, 1407, 1472, 1485
BsrBI CCGCTC 1 cut(s) 630
BssECI CCNNGG 3 cut(s) 162, 1355, 1542
BssMI GATC 9 cut(s) 61, 148, 221, 274, 496, 571, 1005, 1399, 1477
BssNI GRCGYC 1 cut(s) 136
BssT1I CCWWGG 3 cut(s) 162, 1355, 1542
Bst4CI ACNGT 2 cut(s) 650, 739
Bst6I CTCTTC 3 cut(s) 603, 1065, 1226
BstACI GRCGYC 1 cut(s) 136
BstDEI CTNAG 6 cut(s) 13, 211, 375, 474, 845, 1559
BstDSI CCRYGG 1 cut(s) 162
BstEII GGTNACC 1 cut(s) 857
BstF5I GGATG 5 cut(s) 358, 832, 916, 973, 1453
BstFNI CGCG 2 cut(s) 791, 812
BstHHI GCGC 1 cut(s) 725
BstKTI GATC 9 cut(s) 64, 151, 224, 277, 499, 574, 1008, 1402, 1480
BstMAI GTCTC 4 cut(s) 40, 669, 815, 1442
BstMBI GATC 9 cut(s) 61, 148, 221, 274, 496, 571, 1005, 1399, 1477
BstMWI GCNNNNNNNGC 5 cut(s) 101, 104, 113, 1193, 1491
BstPAI GACNNNNGTC 1 cut(s) 213
BstPI GGTNACC 1 cut(s) 857
BstSCI CCNGG 2 cut(s) 1443, 1498
BstSFI CTRYAG 1 cut(s) 99
BstUI CGCG 2 cut(s) 791, 812
BstV1I GCAGC 5 cut(s) 94, 116, 271, 545, 901
BstX2I RGATCY 4 cut(s) 274, 571, 1399, 1477
BstYI RGATCY 4 cut(s) 274, 571, 1399, 1477
Bsu36I CCTNAGG 1 cut(s) 13
BsuRI GGCC 3 cut(s) 785, 1540, 1589
BtgI CCRYGG 1 cut(s) 162
BtgZI GCGATG 2 cut(s) 501, 1578
BtrI CACGTC 1 cut(s) 953
BtsCI GGATG 5 cut(s) 358, 832, 916, 973, 1453
BtsI GCAGTG 2 cut(s) 643, 1245
BtsIMutI CAGTG 3 cut(s) 643, 646, 1245
CfoI GCGC 1 cut(s) 725
Cfr13I GGNCC 5 cut(s) 450, 1349, 1539, 1546, 1588
CseI GACGC 4 cut(s) 144, 797, 818, 1193
Csp6I GTAC 1 cut(s) 651
CviAII CATG 2 cut(s) 163, 981
CviQI GTAC 1 cut(s) 651
DdeI CTNAG 6 cut(s) 13, 211, 375, 474, 845, 1559
DpnI GATC 9 cut(s) 63, 150, 223, 276, 498, 573, 1007, 1401, 1479
DpnII GATC 9 cut(s) 61, 148, 221, 274, 496, 571, 1005, 1399, 1477
Eam1104I CTCTTC 3 cut(s) 603, 1065, 1226
EarI CTCTTC 3 cut(s) 603, 1065, 1226
EciI GGCGGA 1 cut(s) 726
Ecl136II GAGCTC 2 cut(s) 842, 1220
Eco130I CCWWGG 3 cut(s) 162, 1355, 1542
Eco24I GRGCYC 2 cut(s) 844, 1222
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 3 cut(s) 450, 1349, 1546
Eco53kI GAGCTC 2 cut(s) 842, 1220
Eco81I CCTNAGG 1 cut(s) 13
Eco91I GGTNACC 1 cut(s) 857
EcoICRI GAGCTC 2 cut(s) 842, 1220
EcoO65I GGTNACC 1 cut(s) 857
EcoRI GAATTC 1 cut(s) 1261
EcoRV GATATC 1 cut(s) 751
EcoT14I CCWWGG 3 cut(s) 162, 1355, 1542
EcoT22I ATGCAT 2 cut(s) 425, 1288
EcoT38I GRGCYC 2 cut(s) 844, 1222
ErhI CCWWGG 3 cut(s) 162, 1355, 1542
Esp3I CGTCTC 1 cut(s) 815
FaeI CATG 2 cut(s) 166, 984
FaqI GGGAC 2 cut(s) 675, 1090
FatI CATG 2 cut(s) 162, 980
FbaI TGATCA 1 cut(s) 148
FblI GTMKAC 1 cut(s) 217
Fnu4HI GCNGC 7 cut(s) 93, 96, 105, 108, 260, 534, 915
FokI GGATG 5 cut(s) 365, 839, 923, 980, 1460
FriOI GRGCYC 2 cut(s) 844, 1222
Fsp4HI GCNGC 7 cut(s) 93, 96, 105, 108, 260, 534, 915
FspBI CTAG 6 cut(s) 71, 230, 758, 852, 929, 1320
GlaI GCGC 1 cut(s) 724
GluI GCNGC 7 cut(s) 93, 96, 105, 108, 260, 534, 915
HaeIII GGCC 3 cut(s) 785, 1540, 1589
HapII CCGG 6 cut(s) 117, 139, 668, 1445, 1475, 1500
HgaI GACGC 4 cut(s) 144, 797, 818, 1193
HhaI GCGC 1 cut(s) 725
Hin1I GRCGYC 1 cut(s) 136
Hin1II CATG 2 cut(s) 166, 984
Hin6I GCGC 1 cut(s) 723
HinP1I GCGC 1 cut(s) 723
HincII GTYRAC 1 cut(s) 218
HindII GTYRAC 1 cut(s) 218
HindIII AAGCTT 1 cut(s) 1388
HinfI GANTC 9 cut(s) 209, 237, 383, 491, 618, 761, 1158, 1454, 1578
HpaII CCGG 6 cut(s) 117, 139, 668, 1445, 1475, 1500
HphI GGTGA 4 cut(s) 16, 592, 851, 1240
Hpy166II GTNNAC 2 cut(s) 218, 1027
Hpy188I TCNGA 6 cut(s) 266, 496, 501, 775, 1069, 1146
Hpy188III TCNNGA 6 cut(s) 526, 852, 1403, 1475, 1549, 1573
Hpy8I GTNNAC 2 cut(s) 218, 1027
Hpy99I CGWCG 5 cut(s) 138, 222, 791, 950, 998
HpyAV CCTTC 5 cut(s) 14, 140, 707, 1046, 1546
HpyCH4III ACNGT 2 cut(s) 650, 739
HpyCH4IV ACGT 2 cut(s) 952, 1023
HpyCH4V TGCA 8 cut(s) 254, 300, 423, 917, 1286, 1332, 1451, 1568
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 104, 113, 1193, 1491
HpyF3I CTNAG 6 cut(s) 13, 211, 375, 474, 845, 1559
HpySE526I ACGT 2 cut(s) 952, 1023
Hsp92I GRCGYC 1 cut(s) 136
Hsp92II CATG 2 cut(s) 166, 984
HspAI GCGC 1 cut(s) 723
Kpn2I TCCGGA 1 cut(s) 1474
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 9 cut(s) 61, 148, 221, 274, 496, 571, 1005, 1399, 1477
LmnI GCTCC 2 cut(s) 627, 985
Lsp1109I GCAGC 5 cut(s) 94, 116, 271, 545, 901
LweI GCATC 5 cut(s) 410, 901, 926, 1438, 1555
MaeI CTAG 6 cut(s) 71, 230, 758, 852, 929, 1320
MaeII ACGT 2 cut(s) 952, 1023
MaeIII GTNAC 4 cut(s) 562, 857, 931, 1181
MalI GATC 9 cut(s) 63, 150, 223, 276, 498, 573, 1007, 1401, 1479
MbiI CCGCTC 1 cut(s) 630
MboI GATC 9 cut(s) 61, 148, 221, 274, 496, 571, 1005, 1399, 1477
MfeI CAATTG 2 cut(s) 445, 1256
MflI RGATCY 4 cut(s) 274, 571, 1399, 1477
MhlI GDGCHC 3 cut(s) 737, 844, 1222
MluI ACGCGT 1 cut(s) 810
MlyI GAGTC 4 cut(s) 203, 500, 627, 770
MmeI TCCRAC 3 cut(s) 474, 486, 753
Mph1103I ATGCAT 2 cut(s) 425, 1288
MroI TCCGGA 1 cut(s) 1474
MroXI GAANNNNTTC 1 cut(s) 891
MseI TTAA 3 cut(s) 482, 816, 1490
MslI CAYNNNNRTG 3 cut(s) 295, 864, 979
MspA1I CMGCKG 2 cut(s) 98, 107
MspI CCGG 6 cut(s) 117, 139, 668, 1445, 1475, 1500
MspR9I CCNGG 2 cut(s) 1445, 1500
MunI CAATTG 2 cut(s) 445, 1256
Mva1269I GAATGC 2 cut(s) 423, 1286
MvnI CGCG 2 cut(s) 791, 812
MwoI GCNNNNNNNGC 5 cut(s) 101, 104, 113, 1193, 1491
NciI CCSGG 2 cut(s) 1445, 1500
NcoI CCATGG 1 cut(s) 162
NdeII GATC 9 cut(s) 61, 148, 221, 274, 496, 571, 1005, 1399, 1477
NlaIII CATG 2 cut(s) 166, 984
NlaIV GGNNCC 5 cut(s) 276, 452, 987, 1401, 1479
NmuCI GTSAC 2 cut(s) 857, 1181
NsiI ATGCAT 2 cut(s) 425, 1288
PctI GAATGC 2 cut(s) 423, 1286
PdmI GAANNNNTTC 1 cut(s) 891
PfeI GAWTC 5 cut(s) 237, 383, 1158, 1454, 1578
PkrI GCNGC 7 cut(s) 94, 97, 106, 109, 261, 535, 916
PleI GAGTC 4 cut(s) 203, 499, 626, 769
PpsI GAGTC 4 cut(s) 203, 499, 626, 769
PshAI GACNNNNGTC 1 cut(s) 213
Psp124BI GAGCTC 2 cut(s) 844, 1222
PspEI GGTNACC 1 cut(s) 857
PspN4I GGNNCC 5 cut(s) 276, 452, 987, 1401, 1479
PspPI GGNCC 5 cut(s) 450, 1349, 1539, 1546, 1588
PsuI RGATCY 4 cut(s) 274, 571, 1399, 1477
PvuII CAGCTG 1 cut(s) 107
RsaI GTAC 1 cut(s) 652
RsaNI GTAC 1 cut(s) 651
RseI CAYNNNNRTG 3 cut(s) 295, 864, 979
SacI GAGCTC 2 cut(s) 844, 1222
SalI GTCGAC 1 cut(s) 216
SaqAI TTAA 3 cut(s) 482, 816, 1490
SatI GCNGC 7 cut(s) 93, 96, 105, 108, 260, 534, 915
Sau3AI GATC 9 cut(s) 61, 148, 221, 274, 496, 571, 1005, 1399, 1477
Sau96I GGNCC 5 cut(s) 450, 1349, 1539, 1546, 1588
SchI GAGTC 4 cut(s) 203, 500, 627, 770
ScrFI CCNGG 2 cut(s) 1445, 1500
SduI GDGCHC 3 cut(s) 737, 844, 1222
SfaNI GCATC 5 cut(s) 410, 901, 926, 1438, 1555
SfcI CTRYAG 1 cut(s) 99
SinI GGWCC 3 cut(s) 450, 1349, 1546
SmiMI CAYNNNNRTG 3 cut(s) 295, 864, 979
SmlI CTYRAG 2 cut(s) 515, 575
SmoI CTYRAG 2 cut(s) 515, 575
SpeI ACTAGT 1 cut(s) 928
SsiI CCGC 5 cut(s) 93, 96, 630, 711, 791
SspI AATATT 1 cut(s) 1094
SspMI CTAG 6 cut(s) 71, 230, 758, 852, 929, 1320
SstI GAGCTC 2 cut(s) 844, 1222
StyD4I CCNGG 2 cut(s) 1443, 1498
StyI CCWWGG 3 cut(s) 162, 1355, 1542
TaaI ACNGT 2 cut(s) 650, 739
TaiI ACGT 2 cut(s) 955, 1026
TaqI TCGA 9 cut(s) 133, 217, 596, 996, 1008, 1042, 1161, 1535, 1581
TauI GCSGC 2 cut(s) 95, 98
TfiI GAWTC 5 cut(s) 237, 383, 1158, 1454, 1578
Tru1I TTAA 3 cut(s) 482, 816, 1490
Tru9I TTAA 3 cut(s) 482, 816, 1490
TscAI CASTG 3 cut(s) 643, 653, 1245
TseFI GTSAC 2 cut(s) 857, 1181
TseI GCWGC 5 cut(s) 104, 107, 259, 533, 914
Tsp45I GTSAC 2 cut(s) 857, 1181
TspDTI ATGAA 4 cut(s) 297, 384, 386, 884
TspGWI ACGGA 6 cut(s) 668, 839, 923, 963, 1228, 1392
TspRI CASTG 3 cut(s) 643, 653, 1245
VpaK11BI GGWCC 3 cut(s) 450, 1349, 1546
XapI RAATTY 2 cut(s) 410, 1261
XbaI TCTAGA 1 cut(s) 851
XmiI GTMKAC 1 cut(s) 217
XmnI GAANNNNTTC 1 cut(s) 891
XspI CTAG 6 cut(s) 71, 230, 758, 852, 929, 1320
Zsp2I ATGCAT 2 cut(s) 425, 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.