Rh4AG032300

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007045
Physical Location & Seq
Reverse (-)
6946617 .. 6954084
7468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG032300.1

Sequence Viewer

Length: 1626 bp
ATGAGGTGGGGCAACCGCACCATCCAAGGAAAGGGGTTGCCTAAAGATTTAATCTTGGAGAATGGTGGGCATGAGACAATCAAGTACATCAAGCACCTACTGGCCGCCTCGTTTGTTAGTGGTGCAGCGGCGGTCACCGCCCCTTATCACCAGAAAAAGGCCGCCTTGACAGCTCCACATGAAATCTTTCCAAGTCTCAACAGGACAGACTCCGGCCGTGTTGGAAACATCGAGAGATTCTCCGACTATGTTGCTAGGCAGCTAGGATTTTCGGACGCAAATGAGTGCCCCAAGATGTGCAAGTTGGCATATGACTACTTGAAAAACTCCGAAGGGTGTAAAGACAGCATCTACGAGTATTTCGCTTCCGAAGAAGATGCAGAGTCTGTGTACATCGAGTTTACAGAAGAATTGGATCGATGCATCCTTGGTTATTTTGCGTTTCATTGGAGTCAAACTTCTCATATGATTAGCCAGGTTTTTGATGTGGAATCTGAGAGGAGAACGAAGCTCAAAGACTATGTACTTGCAGCTACCAGGAAACAGAGATTTGAGAGGATAAGCAAGGACCTAAAGGTGACAAGGGTGTTCTCTACTTTGGTAGAGGAAATGAAAGTAATTGGCAACACATCAAAATGCACAGAAGTAATGAAGCCTTTGGCACATAGTGAGAGGAGCCCTGTGCTCCTTCTCATGGGTGGTGGAATGGGAGCTGGCAAAAGCACTGTCCTCAAAGACATTCTCAAAGAGGCATTCTGGACAGAAGCCAAGAAAAATGCGGTAGTTGTAGAGGCAGACGCTTTCAAGGAGACGGATGTTCTTTACAGAGCGTTAAGCCTGAAAGGTCATCATCATGACATGCTCCAATCTGCTGAATTGGTGCACCAATCATCCACCGATGCTGCATCGTCCCTCCTAGTGACCGCACTCAACGAAGGACGGGATGTGATCATGGATGGCACTCTATCATGGGCACCCTTTGTCGATCAGACAATAGCAATGGCCCGAAATGTTCACAAGTGCCGTTACCGGATGGGAGTTGGATACAAGGTGGCTGATGATGGCACCATCACTGAAAATTATTGGGAACAAGTAAGTGAAGAAGAAGAGGATGAGCATCAAAAGTTAGAAAAAGGAGGGAAACCCCAAAGAAAACCGTACAGAATAGAGTTGGTTGGTGTGGTTTGTGATGCTTATTTAGCTGTTATTAGAGGCATTAGGAGAGCTATAATGATTCATAGGGCAGTGAGGGTAAATTCACAGATTCAATCCCACCAGAGATTTGCTAAAGCATTTACTGGATATTGCCAGCTTGTCGATAATGCCAGGCTCTTCTGCACTAATGCTATGGCTGGCCCGCCTAAGCTGATAGGATGGAAGGATGGAGATAACAACCTGCTAGAGGATCCGAAAGAATTCAAATGCTTGACAGATTTGAGCAATTTGAACTCTAAAGCCGATTCGATATACGAGCTTTACACGCACAACCCGGGCCTTATACTGGAACCTGGTTCTCTTTGGAAAGAAGCTGTTTTGTCCCCTGCAAGGCCAAGCATTCAGCGCAAGCTAAAGGCTTGCATTCAAAGATTTGAAATTTCAAGTATTACTGCTGACGAATTAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

541

Amino Acids

60.87

Weight (kDa)

6.97

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 219 - 336 1.1e-14 Zeta toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1404
AccB1I GGYRCC 2 cut(s) 973, 1064
AciI CCGC 9 cut(s) 16, 105, 128, 131, 138, 162, 779, 924, 1358
AclWI GGATC 3 cut(s) 423, 1400, 1413
AcoI YGGCCR 2 cut(s) 102, 214
AcsI RAATTY 3 cut(s) 1255, 1415, 1593
AdeI CACNNNGTG 1 cut(s) 668
AfaI GTAC 4 cut(s) 86, 392, 525, 1160
AfiI CCNNNNNNNGG 6 cut(s) 31, 157, 694, 1402, 1501, 1545
AgsI TTSAA 8 cut(s) 322, 805, 1268, 1420, 1447, 1583, 1592, 1599
AjnI CCWGG 4 cut(s) 474, 536, 1325, 1507
Alw21I GWGCWC 2 cut(s) 687, 885
Alw26I GTCTC 3 cut(s) 68, 200, 803
Alw44I GTGCAC 1 cut(s) 881
AlwI GGATC 3 cut(s) 423, 1400, 1413
AlwNI CAGNNNCTG 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 1489
AoxI GGCC 7 cut(s) 102, 159, 214, 1002, 1354, 1492, 1547
ApaLI GTGCAC 1 cut(s) 881
ApeKI GCWGC 4 cut(s) 125, 259, 530, 902
ApoI RAATTY 3 cut(s) 1255, 1415, 1593
ArsI GACNNNNNNTTYG 2 cut(s) 711, 743
Asp700I GAANNNNTTC 2 cut(s) 186, 1415
AspLEI GCGC 1 cut(s) 1563
AspS9I GGNCC 4 cut(s) 568, 1003, 1355, 1492
AsuC2I CCSGG 2 cut(s) 1490, 1491
AsuHPI GGTGA 3 cut(s) 127, 140, 589
AvaI CYCGRG 1 cut(s) 1489
AvaII GGWCC 1 cut(s) 568
BaeGI GKGCMC 3 cut(s) 290, 885, 976
BamHI GGATCC 1 cut(s) 1405
BanI GGYRCC 2 cut(s) 973, 1064
BanII GRGCYC 1 cut(s) 680
Bbv12I GWGCWC 2 cut(s) 687, 885
BbvI GCAGC 4 cut(s) 137, 271, 542, 889
BccI CCATC 7 cut(s) 29, 950, 1027, 1055, 1076, 1368, 1376
BceAI ACGGC 2 cut(s) 201, 1008
BcgI CGANNNNNNTGC 4 cut(s) 233, 267, 359, 393
BciT130I CCWGG 4 cut(s) 476, 538, 1327, 1509
BciVI GTATCC 1 cut(s) 1037
BclI TGATCA 1 cut(s) 948
BcnI CCSGG 2 cut(s) 1490, 1491
BcoDI GTCTC 3 cut(s) 68, 200, 803
BfaI CTAG 4 cut(s) 255, 263, 917, 1400
BfuAI ACCTGC 1 cut(s) 1404
BfuI GTATCC 1 cut(s) 1037
BisI GCNGC 7 cut(s) 105, 126, 129, 162, 260, 531, 903
BlsI GCNGC 7 cut(s) 106, 127, 130, 163, 261, 532, 904
Bme1390I CCNGG 6 cut(s) 476, 538, 1327, 1490, 1491, 1509
Bme18I GGWCC 1 cut(s) 568
BmeT110I CYCGRG 1 cut(s) 1489
BmgT120I GGNCC 4 cut(s) 568, 1003, 1355, 1492
BmiI GGNNCC 5 cut(s) 677, 975, 1066, 1407, 1506
BmrFI CCNGG 6 cut(s) 476, 538, 1327, 1490, 1491, 1509
BmsI GCATC 8 cut(s) 357, 367, 410, 432, 889, 914, 1126, 1180
BplI GAGNNNNNCTC 2 cut(s) 224, 256
Bpu10I CCTNAGC 1 cut(s) 1362
BpuMI CCSGG 2 cut(s) 1490, 1491
Bsa29I ATCGAT 1 cut(s) 418
BsaBI GATNNNNATC 1 cut(s) 1116
BsaJI CCNNGG 3 cut(s) 25, 427, 1489
BsaWI WCCGGW 1 cut(s) 1029
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 157, 694, 1402, 1501, 1545
Bse1I ACTGG 3 cut(s) 105, 1303, 1506
Bse3DI GCAATG 1 cut(s) 1005
Bse8I GATNNNNATC 1 cut(s) 1116
BseBI CCWGG 4 cut(s) 476, 538, 1327, 1509
BseCI ATCGAT 1 cut(s) 418
BseDI CCNNGG 3 cut(s) 25, 427, 1489
BseJI GATNNNNATC 1 cut(s) 1116
BseLI CCNNNNNNNGG 6 cut(s) 31, 157, 694, 1402, 1501, 1545
BseMI GCAATG 1 cut(s) 1005
BseMII CTCAG 1 cut(s) 486
BseNI ACTGG 3 cut(s) 105, 1303, 1506
BseRI GAGGAG 2 cut(s) 514, 688
BseSI GKGCMC 3 cut(s) 290, 885, 976
BseX3I CGGCCG 1 cut(s) 214
BseXI GCAGC 4 cut(s) 137, 271, 542, 889
BsgI GTGCAG 2 cut(s) 144, 1321
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 7 cut(s) 104, 161, 216, 1004, 1356, 1494, 1549
BshNI GGYRCC 2 cut(s) 973, 1064
BshVI ATCGAT 1 cut(s) 418
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 2 cut(s) 687, 885
BsiHKCI CYCGRG 1 cut(s) 1489
BsiSI CCGG 3 cut(s) 213, 1030, 1490
BslFI GGGAC 2 cut(s) 895, 1522
BslI CCNNNNNNNGG 6 cut(s) 31, 157, 694, 1402, 1501, 1545
BsmAI GTCTC 3 cut(s) 68, 200, 803
BsmBI CGTCTC 1 cut(s) 803
BsmFI GGGAC 2 cut(s) 895, 1522
BsmI GAATGC 3 cut(s) 752, 1554, 1578
BsnI GGCC 7 cut(s) 104, 161, 216, 1004, 1356, 1494, 1549
BsoBI CYCGRG 1 cut(s) 1489
Bsp1286I GDGCHC 5 cut(s) 290, 680, 687, 885, 976
Bsp1407I TGTACA 1 cut(s) 390
Bsp143I GATC 4 cut(s) 415, 948, 985, 1405
BspACI CCGC 9 cut(s) 16, 105, 128, 131, 138, 162, 779, 924, 1358
BspANI GGCC 7 cut(s) 104, 161, 216, 1004, 1356, 1494, 1549
BspCNI CTCAG 1 cut(s) 487
BspDI ATCGAT 1 cut(s) 418
BspHI TCATGA 1 cut(s) 853
BspLI GGNNCC 5 cut(s) 677, 975, 1066, 1407, 1506
BspMI ACCTGC 1 cut(s) 1404
BspPI GGATC 3 cut(s) 423, 1400, 1413
BspQI GCTCTTC 1 cut(s) 1337
BspT107I GGYRCC 2 cut(s) 973, 1064
BsrDI GCAATG 1 cut(s) 1005
BsrGI TGTACA 1 cut(s) 390
BsrI ACTGG 3 cut(s) 105, 1303, 1506
BssECI CCNNGG 3 cut(s) 25, 427, 1489
BssMI GATC 4 cut(s) 415, 948, 985, 1405
BssT1I CCWWGG 2 cut(s) 25, 427
Bst2UI CCWGG 4 cut(s) 476, 538, 1327, 1509
Bst4CI ACNGT 2 cut(s) 727, 1158
Bst6I CTCTTC 2 cut(s) 1101, 1337
BstAUI TGTACA 1 cut(s) 390
BstC8I GCNNGC 6 cut(s) 715, 1310, 1354, 1358, 1565, 1576
BstDEI CTNAG 2 cut(s) 495, 1362
BstEII GGTNACC 1 cut(s) 133
BstENI CCTNNNNNAGG 1 cut(s) 1400
BstHHI GCGC 1 cut(s) 1563
BstKTI GATC 4 cut(s) 418, 951, 988, 1408
BstMAI GTCTC 3 cut(s) 68, 200, 803
BstMBI GATC 4 cut(s) 415, 948, 985, 1405
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 5 cut(s) 137, 170, 1199, 1480, 1560
BstNI CCWGG 4 cut(s) 476, 538, 1327, 1509
BstNSI RCATGY 1 cut(s) 862
BstPI GGTNACC 1 cut(s) 133
BstSCI CCNGG 6 cut(s) 474, 536, 1325, 1488, 1489, 1507
BstSLI GKGCMC 3 cut(s) 290, 885, 976
BstV1I GCAGC 4 cut(s) 137, 271, 542, 889
BstX2I RGATCY 1 cut(s) 1405
BstYI RGATCY 1 cut(s) 1405
BstZI CGGCCG 1 cut(s) 214
Bsu15I ATCGAT 1 cut(s) 418
BsuI GTATCC 1 cut(s) 1037
BsuRI GGCC 7 cut(s) 104, 161, 216, 1004, 1356, 1494, 1549
BsuTUI ATCGAT 1 cut(s) 418
BtsI GCAGTG 1 cut(s) 1251
BtsIMutI CAGTG 3 cut(s) 723, 1071, 1251
BveI ACCTGC 1 cut(s) 1404
Cac8I GCNNGC 6 cut(s) 715, 1310, 1354, 1358, 1565, 1576
CaiI CAGNNNCTG 1 cut(s) 386
CciI TCATGA 1 cut(s) 853
CfoI GCGC 1 cut(s) 1563
Cfr13I GGNCC 4 cut(s) 568, 1003, 1355, 1492
Cfr9I CCCGGG 1 cut(s) 1489
ClaI ATCGAT 1 cut(s) 418
CseI GACGC 2 cut(s) 284, 806
CsiI ACCWGGT 1 cut(s) 1507
Csp6I GTAC 4 cut(s) 85, 391, 524, 1159
CviAII CATG 7 cut(s) 71, 179, 694, 854, 859, 952, 969
CviQI GTAC 4 cut(s) 85, 391, 524, 1159
DdeI CTNAG 2 cut(s) 495, 1362
DpnI GATC 4 cut(s) 417, 950, 987, 1407
DpnII GATC 4 cut(s) 415, 948, 985, 1405
DraIII CACNNNGTG 1 cut(s) 668
EaeI YGGCCR 2 cut(s) 102, 214
EagI CGGCCG 1 cut(s) 214
Eam1104I CTCTTC 2 cut(s) 1101, 1337
EarI CTCTTC 2 cut(s) 1101, 1337
EclXI CGGCCG 1 cut(s) 214
Eco130I CCWWGG 2 cut(s) 25, 427
Eco24I GRGCYC 1 cut(s) 680
Eco47I GGWCC 1 cut(s) 568
Eco52I CGGCCG 1 cut(s) 214
Eco88I CYCGRG 1 cut(s) 1489
Eco91I GGTNACC 1 cut(s) 133
EcoNI CCTNNNNNAGG 1 cut(s) 1400
EcoO109I RGGNCCY 1 cut(s) 568
EcoO65I GGTNACC 1 cut(s) 133
EcoRI GAATTC 1 cut(s) 1415
EcoRII CCWGG 4 cut(s) 474, 536, 1325, 1507
EcoT14I CCWWGG 2 cut(s) 25, 427
EcoT22I ATGCAT 1 cut(s) 425
EcoT38I GRGCYC 1 cut(s) 680
ErhI CCWWGG 2 cut(s) 25, 427
Esp3I CGTCTC 1 cut(s) 803
FaeI CATG 7 cut(s) 74, 182, 697, 857, 862, 955, 972
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FaqI GGGAC 2 cut(s) 895, 1522
FatI CATG 7 cut(s) 70, 178, 693, 853, 858, 951, 968
FauI CCCGC 1 cut(s) 1365
FauNDI CATATG 2 cut(s) 310, 465
FbaI TGATCA 1 cut(s) 948
Fnu4HI GCNGC 7 cut(s) 105, 126, 129, 162, 260, 531, 903
FriOI GRGCYC 1 cut(s) 680
Fsp4HI GCNGC 7 cut(s) 105, 126, 129, 162, 260, 531, 903
FspBI CTAG 4 cut(s) 255, 263, 917, 1400
GlaI GCGC 1 cut(s) 1562
GluI GCNGC 7 cut(s) 105, 126, 129, 162, 260, 531, 903
HaeIII GGCC 7 cut(s) 104, 161, 216, 1004, 1356, 1494, 1549
HapII CCGG 3 cut(s) 213, 1030, 1490
HgaI GACGC 2 cut(s) 284, 806
HhaI GCGC 1 cut(s) 1563
Hin1II CATG 7 cut(s) 74, 182, 697, 857, 862, 955, 972
Hin6I GCGC 1 cut(s) 1561
HinP1I GCGC 1 cut(s) 1561
HinfI GANTC 8 cut(s) 209, 237, 383, 451, 491, 1234, 1264, 1460
HpaII CCGG 3 cut(s) 213, 1030, 1490
HphI GGTGA 3 cut(s) 127, 140, 589
Hpy166II GTNNAC 4 cut(s) 391, 402, 883, 1015
Hpy188I TCNGA 7 cut(s) 244, 274, 331, 370, 496, 990, 1410
Hpy188III TCNNGA 3 cut(s) 232, 757, 854
Hpy8I GTNNAC 4 cut(s) 391, 402, 883, 1015
HpyAV CCTTC 4 cut(s) 326, 698, 929, 1372
HpyCH4III ACNGT 2 cut(s) 727, 1158
HpyF10VI GCNNNNNNNGC 5 cut(s) 137, 170, 1199, 1480, 1560
HpyF3I CTNAG 2 cut(s) 495, 1362
Hsp92II CATG 7 cut(s) 74, 182, 697, 857, 862, 955, 972
HspAI GCGC 1 cut(s) 1561
Ksp22I TGATCA 1 cut(s) 948
Kzo9I GATC 4 cut(s) 415, 948, 985, 1405
LguI GCTCTTC 1 cut(s) 1337
LmnI GCTCC 5 cut(s) 178, 675, 690, 710, 867
Lsp1109I GCAGC 4 cut(s) 137, 271, 542, 889
LweI GCATC 8 cut(s) 357, 367, 410, 432, 889, 914, 1126, 1180
MabI ACCWGGT 1 cut(s) 1507
MaeI CTAG 4 cut(s) 255, 263, 917, 1400
MaeIII GTNAC 4 cut(s) 133, 577, 919, 1025
MalI GATC 4 cut(s) 417, 950, 987, 1407
MboI GATC 4 cut(s) 415, 948, 985, 1405
MboII GAAGA 7 cut(s) 383, 386, 419, 1112, 1115, 1118, 1324
MflI RGATCY 1 cut(s) 1405
MhlI GDGCHC 5 cut(s) 290, 680, 687, 885, 976
MluCI AATT 9 cut(s) 410, 618, 875, 1078, 1255, 1415, 1441, 1593, 1616
MlyI GAGTC 3 cut(s) 203, 392, 460
MmeI TCCRAC 3 cut(s) 202, 267, 1021
Mph1103I ATGCAT 1 cut(s) 425
MroXI GAANNNNTTC 2 cut(s) 186, 1415
MseI TTAA 4 cut(s) 50, 833, 1619, 1624
MslI CAYNNNNRTG 2 cut(s) 634, 852
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 3 cut(s) 213, 1030, 1490
MspR9I CCNGG 6 cut(s) 476, 538, 1327, 1490, 1491, 1509
Mva1269I GAATGC 3 cut(s) 752, 1554, 1578
MvaI CCWGG 4 cut(s) 476, 538, 1327, 1509
MwoI GCNNNNNNNGC 5 cut(s) 137, 170, 1199, 1480, 1560
NciI CCSGG 2 cut(s) 1490, 1491
NdeI CATATG 2 cut(s) 310, 465
NdeII GATC 4 cut(s) 415, 948, 985, 1405
NlaIII CATG 7 cut(s) 74, 182, 697, 857, 862, 955, 972
NlaIV GGNNCC 5 cut(s) 677, 975, 1066, 1407, 1506
NmuCI GTSAC 3 cut(s) 133, 577, 919
NsiI ATGCAT 1 cut(s) 425
NspI RCATGY 1 cut(s) 862
PagI TCATGA 1 cut(s) 853
PciSI GCTCTTC 1 cut(s) 1337
PctI GAATGC 3 cut(s) 752, 1554, 1578
PdmI GAANNNNTTC 2 cut(s) 186, 1415
PfeI GAWTC 5 cut(s) 237, 491, 1234, 1264, 1460
PkrI GCNGC 7 cut(s) 106, 127, 130, 163, 261, 532, 904
PleI GAGTC 3 cut(s) 203, 391, 459
PpsI GAGTC 3 cut(s) 203, 391, 459
PpuMI RGGWCCY 1 cut(s) 568
Psp5II RGGWCCY 1 cut(s) 568
Psp6I CCWGG 4 cut(s) 474, 536, 1325, 1507
PspEI GGTNACC 1 cut(s) 133
PspGI CCWGG 4 cut(s) 474, 536, 1325, 1507
PspN4I GGNNCC 5 cut(s) 677, 975, 1066, 1407, 1506
PspPI GGNCC 4 cut(s) 568, 1003, 1355, 1492
PspPPI RGGWCCY 1 cut(s) 568
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 1 cut(s) 1405
RsaI GTAC 4 cut(s) 86, 392, 525, 1160
RsaNI GTAC 4 cut(s) 85, 391, 524, 1159
RseI CAYNNNNRTG 2 cut(s) 634, 852
SapI GCTCTTC 1 cut(s) 1337
SaqAI TTAA 4 cut(s) 50, 833, 1619, 1624
SatI GCNGC 7 cut(s) 105, 126, 129, 162, 260, 531, 903
Sau3AI GATC 4 cut(s) 415, 948, 985, 1405
Sau96I GGNCC 4 cut(s) 568, 1003, 1355, 1492
SchI GAGTC 3 cut(s) 203, 392, 460
ScrFI CCNGG 6 cut(s) 476, 538, 1327, 1490, 1491, 1509
SduI GDGCHC 5 cut(s) 290, 680, 687, 885, 976
SexAI ACCWGGT 1 cut(s) 1507
SfaNI GCATC 8 cut(s) 357, 367, 410, 432, 889, 914, 1126, 1180
SinI GGWCC 1 cut(s) 568
SmaI CCCGGG 1 cut(s) 1491
SmiMI CAYNNNNRTG 2 cut(s) 634, 852
Sse9I AATT 9 cut(s) 410, 618, 875, 1078, 1255, 1415, 1441, 1593, 1616
SsiI CCGC 9 cut(s) 16, 105, 128, 131, 138, 162, 779, 924, 1358
SspMI CTAG 4 cut(s) 255, 263, 917, 1400
StyD4I CCNGG 6 cut(s) 474, 536, 1325, 1488, 1489, 1507
StyI CCWWGG 2 cut(s) 25, 427
TaaI ACNGT 2 cut(s) 727, 1158
TaqI TCGA 6 cut(s) 231, 396, 418, 984, 1317, 1463
TasI AATT 9 cut(s) 410, 618, 875, 1078, 1255, 1415, 1441, 1593, 1616
TatI WGTACW 3 cut(s) 84, 390, 523
TauI GCSGC 3 cut(s) 107, 131, 164
TfiI GAWTC 5 cut(s) 237, 491, 1234, 1264, 1460
Tru1I TTAA 4 cut(s) 50, 833, 1619, 1624
Tru9I TTAA 4 cut(s) 50, 833, 1619, 1624
TscAI CASTG 3 cut(s) 730, 1078, 1251
TseFI GTSAC 3 cut(s) 133, 577, 919
TseI GCWGC 4 cut(s) 125, 259, 530, 902
Tsp45I GTSAC 3 cut(s) 133, 577, 919
TspDTI ATGAA 5 cut(s) 195, 434, 626, 665, 1226
TspGWI ACGGA 1 cut(s) 827
TspMI CCCGGG 1 cut(s) 1489
TspRI CASTG 3 cut(s) 730, 1078, 1251
VneI GTGCAC 1 cut(s) 881
VpaK11BI GGWCC 1 cut(s) 568
XagI CCTNNNNNAGG 1 cut(s) 1400
XapI RAATTY 3 cut(s) 1255, 1415, 1593
XceI RCATGY 1 cut(s) 862
XmaI CCCGGG 1 cut(s) 1489
XmnI GAANNNNTTC 2 cut(s) 186, 1415
XspI CTAG 4 cut(s) 255, 263, 917, 1400
Zsp2I ATGCAT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.