pycom05g19030

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007045
Physical Location & Seq
Forward (+)
21951449 .. 21951991
543 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g19030.1

Sequence Viewer

Length: 543 bp
ATGCATATCCCAAATTCCCCTTCTCATCTTTTCACGATCCAAATCAAACAAAAACAAAGGAAGCAAAAAAAAAACAAAAGAAAAATTCATACATTCGTTCTTCAAATCTTAGCAGTTTTCCTGGAATTTGTAAGAGACATGCAGAAAGGTGAGAAATATCATGGAACAAAACTAGTCACCCTCTTGATATTGGCCTCTTCCGTCGCTCTTTGCTTCCACCCTTCATGCAAAAACCTCTCGCAATTTTCATTCCCCGTCACCTCTCCCTTTTCTTCAGATCACACCTTCTCTCACATCCTTGTAGCCTCCTCCATCGGCATCATCGTCGCCGCCGCCCTTCATTACCGCCTCCGAAACCACAAGGATCACAAGATCGTCCCCCGCCTCAAGGTTTCCGACTCAGTCCGCGTTCATCAGAAGCTCGAGAGGTTCCCCCACTACGTAGGTAATGATTACGTGATCGTCCGCTTGTTTTTCATGGCCTTTGCGATCTGCAAGGTGTGTTTTGTGGTTGAATGTTTTCGGCGTTGTGGGTGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

181

Amino Acids

20.77

Weight (kDa)

10.04

Isoelectric Point (pI)

55.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 408
AciI CCGC 6 cut(s) 330, 333, 346, 382, 406, 466
AclWI GGATC 2 cut(s) 31, 372
AcsI RAATTY 3 cut(s) 13, 84, 125
AcuI CTGAAG 1 cut(s) 258
AfiI CCNNNNNNNGG 1 cut(s) 388
AgsI TTSAA 2 cut(s) 104, 515
AhlI ACTAGT 1 cut(s) 172
AjnI CCWGG 1 cut(s) 120
AjuI GAANNNNNNNTTGG 1 cut(s) 36
AluBI AGCT 2 cut(s) 421, 540
AluI AGCT 2 cut(s) 421, 540
Alw26I GTCTC 1 cut(s) 129
AlwI GGATC 2 cut(s) 31, 372
Ama87I CYCGRG 1 cut(s) 422
AoxI GGCC 2 cut(s) 192, 480
ApeKI GCWGC 1 cut(s) 537
ApoI RAATTY 3 cut(s) 13, 84, 125
Asp700I GAANNNNTTC 1 cut(s) 519
AsuHPI GGTGA 3 cut(s) 161, 169, 250
AvaI CYCGRG 1 cut(s) 422
BccI CCATC 1 cut(s) 320
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BciT130I CCWGG 1 cut(s) 122
BcoDI GTCTC 1 cut(s) 129
BcuI ACTAGT 1 cut(s) 172
BfaI CTAG 2 cut(s) 173, 541
BisI GCNGC 3 cut(s) 330, 333, 538
BlsI GCNGC 3 cut(s) 331, 334, 539
Bme1390I CCNGG 1 cut(s) 122
BmeT110I CYCGRG 1 cut(s) 422
BmiI GGNNCC 1 cut(s) 431
BmrFI CCNGG 1 cut(s) 122
BmsI GCATC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 371
BsaAI YACGTR 2 cut(s) 442, 457
BsaBI GATNNNNATC 1 cut(s) 41
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse8I GATNNNNATC 1 cut(s) 41
BseBI CCWGG 1 cut(s) 122
BseGI GGATG 1 cut(s) 294
BseJI GATNNNNATC 1 cut(s) 41
BseLI CCNNNNNNNGG 1 cut(s) 388
BseMII CTCAG 1 cut(s) 414
BseRI GAGGAG 1 cut(s) 298
Bsh1236I CGCG 1 cut(s) 408
BshFI GGCC 2 cut(s) 194, 482
BsiHKCI CYCGRG 1 cut(s) 422
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 1 cut(s) 388
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 1 cut(s) 362
BsnI GGCC 2 cut(s) 194, 482
BsoBI CYCGRG 1 cut(s) 422
Bsp143I GATC 6 cut(s) 36, 277, 364, 372, 459, 489
BspACI CCGC 6 cut(s) 330, 333, 346, 382, 406, 466
BspANI GGCC 2 cut(s) 194, 482
BspCNI CTCAG 1 cut(s) 413
BspFNI CGCG 1 cut(s) 408
BspLI GGNNCC 1 cut(s) 431
BspPI GGATC 2 cut(s) 31, 372
BssMI GATC 6 cut(s) 36, 277, 364, 372, 459, 489
Bst2UI CCWGG 1 cut(s) 122
Bst6I CTCTTC 1 cut(s) 202
BstBAI YACGTR 2 cut(s) 442, 457
BstDEI CTNAG 2 cut(s) 109, 400
BstF5I GGATG 1 cut(s) 294
BstFNI CGCG 1 cut(s) 408
BstKTI GATC 6 cut(s) 39, 280, 367, 375, 462, 492
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 6 cut(s) 36, 277, 364, 372, 459, 489
BstNI CCWGG 1 cut(s) 122
BstNSI RCATGY 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 120
BstSNI TACGTA 1 cut(s) 442
BstUI CGCG 1 cut(s) 408
BsuRI GGCC 2 cut(s) 194, 482
BtsCI GGATG 1 cut(s) 294
CviAII CATG 4 cut(s) 139, 161, 225, 478
CviJI RGCY 5 cut(s) 194, 305, 421, 482, 540
CviKI_1 RGCY 5 cut(s) 194, 305, 421, 482, 540
DdeI CTNAG 2 cut(s) 109, 400
DpnI GATC 6 cut(s) 38, 279, 366, 374, 461, 491
DpnII GATC 6 cut(s) 36, 277, 364, 372, 459, 489
Eam1104I CTCTTC 1 cut(s) 202
EarI CTCTTC 1 cut(s) 202
Eco105I TACGTA 1 cut(s) 442
Eco57I CTGAAG 1 cut(s) 258
Eco88I CYCGRG 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 120
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 4 cut(s) 142, 164, 228, 481
FaiI YATR 6 cut(s) 6, 90, 140, 162, 226, 479
FaqI GGGAC 1 cut(s) 362
FatI CATG 4 cut(s) 138, 160, 224, 477
FauI CCCGC 1 cut(s) 389
Fnu4HI GCNGC 3 cut(s) 330, 333, 538
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 3 cut(s) 330, 333, 538
FspBI CTAG 2 cut(s) 173, 541
GluI GCNGC 3 cut(s) 330, 333, 538
HaeIII GGCC 2 cut(s) 194, 482
Hin1II CATG 4 cut(s) 142, 164, 228, 481
HinfI GANTC 1 cut(s) 398
HphI GGTGA 3 cut(s) 161, 169, 250
Hpy188I TCNGA 4 cut(s) 277, 353, 397, 417
Hpy188III TCNNGA 3 cut(s) 34, 184, 424
Hpy99I CGWCG 2 cut(s) 206, 329
HpyAV CCTTC 4 cut(s) 30, 231, 295, 347
HpyCH4IV ACGT 2 cut(s) 441, 456
HpyCH4V TGCA 5 cut(s) 4, 142, 228, 495, 537
HpyF3I CTNAG 2 cut(s) 109, 400
HpySE526I ACGT 2 cut(s) 441, 456
Hsp92II CATG 4 cut(s) 142, 164, 228, 481
Kzo9I GATC 6 cut(s) 36, 277, 364, 372, 459, 489
LpnPI CCDG 2 cut(s) 107, 134
LweI GCATC 1 cut(s) 327
MaeI CTAG 2 cut(s) 173, 541
MaeII ACGT 2 cut(s) 441, 456
MaeIII GTNAC 2 cut(s) 175, 256
MalI GATC 6 cut(s) 38, 279, 366, 374, 461, 491
MboI GATC 6 cut(s) 36, 277, 364, 372, 459, 489
MboII GAAGA 3 cut(s) 92, 189, 264
MluCI AATT 4 cut(s) 13, 84, 125, 242
MlyI GAGTC 1 cut(s) 392
MmeI TCCRAC 1 cut(s) 420
MnlI CCTC 9 cut(s) 191, 205, 245, 271, 316, 319, 359, 395, 420
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 519
MspR9I CCNGG 1 cut(s) 122
MvaI CCWGG 1 cut(s) 122
MvnI CGCG 1 cut(s) 408
NdeII GATC 6 cut(s) 36, 277, 364, 372, 459, 489
NlaIII CATG 4 cut(s) 142, 164, 228, 481
NlaIV GGNNCC 1 cut(s) 431
NmuCI GTSAC 2 cut(s) 175, 256
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 142
PaeR7I CTCGAG 1 cut(s) 422
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 519
PflFI GACNNNGTC 1 cut(s) 401
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 3 cut(s) 331, 334, 539
PleI GAGTC 1 cut(s) 392
PpsI GAGTC 1 cut(s) 392
Ppu21I YACGTR 2 cut(s) 442, 457
Psp6I CCWGG 1 cut(s) 120
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 1 cut(s) 431
PsyI GACNNNGTC 1 cut(s) 401
SatI GCNGC 3 cut(s) 330, 333, 538
Sau3AI GATC 6 cut(s) 36, 277, 364, 372, 459, 489
SchI GAGTC 1 cut(s) 392
ScrFI CCNGG 1 cut(s) 122
SfaNI GCATC 1 cut(s) 327
Sfr274I CTCGAG 1 cut(s) 422
SlaI CTCGAG 1 cut(s) 422
SmlI CTYRAG 2 cut(s) 386, 422
SmoI CTYRAG 2 cut(s) 386, 422
SnaBI TACGTA 1 cut(s) 442
SpeI ACTAGT 1 cut(s) 172
Sse9I AATT 4 cut(s) 13, 84, 125, 242
SsiI CCGC 6 cut(s) 330, 333, 346, 382, 406, 466
SspMI CTAG 2 cut(s) 173, 541
StyD4I CCNGG 1 cut(s) 120
TaiI ACGT 2 cut(s) 444, 459
TaqI TCGA 1 cut(s) 423
TasI AATT 4 cut(s) 13, 84, 125, 242
TauI GCSGC 2 cut(s) 332, 335
TseFI GTSAC 2 cut(s) 175, 256
TseI GCWGC 1 cut(s) 537
Tsp45I GTSAC 2 cut(s) 175, 256
TspDTI ATGAA 6 cut(s) 77, 213, 237, 329, 401, 466
TspGWI ACGGA 1 cut(s) 190
Tth111I GACNNNGTC 1 cut(s) 401
XapI RAATTY 3 cut(s) 13, 84, 125
XceI RCATGY 1 cut(s) 142
XhoI CTCGAG 1 cut(s) 422
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 2 cut(s) 173, 541
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.