Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010222

Physical Location & Seq

Genomic Coordinates Forward (+)

4153641 .. 4157653

Gene Structure 4013 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G12240.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1995 bp

ATGGCGAGCTCCGATGCTCTCTTGCCAATCTCCGCCAGAGAAGAAGAACCATTATGTCCTTACACGAGATTACCAATGGCCGACCCGAATCAAGAAACCCATGGCCCCCGGAGAAGAAGACCCTTTAAAGGTCTCCTCGCCGTCTCATTTGGTCTCTTGTTCATCGCCTTTTACGTCGCTCTCATCGCCACACACGACGGATCTAGATCCAACGACGAAGGGATCGATGAAACAGAGACGATAACGTCACGTGCACGTCTTGCTGGTGTGTCGGAGAAACGTAACGATGGGTTATGGAAACTTTCCGGTGATCGGAACACGCCGGCGTTTGAATGGAACAATAGTATGTTGTCGTGGCAACGAACGGCGTTTCATTTTCAGCCTGAGCAAAATTGGATGAACGATCCTAACGGTCCATTGTTCTACAAGGGATGGTACCATTTCTTCTACCAATATAACCCAAACGCAGCCGTATGGGGTGACATTGTTTGGGGTCACGCCGTGTCTAGGGACCTAATCCATTGGGTCCATTTGCCCATAGCCATGGTCGCTGATCAATGGTACGACTCCAACGGTGTGTGGACCGGCTCAGCCACATTTCTCCCTGATGGCTCTATAGTCATGCTCTATACCGGTTCCACCGACAAAGCGGTGCAGGTCCAAAACCTTGCCTACCCTGAAGACCCCAACGACCCACTTCTGTTGAAATGGGTCAAGTTCCCGGGGAACCCGGTTCTAGTACCTCCGCCCGGTATCCTCCCTAAGGACTTCCGTGACCCAACGACTGCATGGAAGACATCAGAAGGAAAATGGCGGATCACGATTGGTTCCAAGCTCAACAAAACTGGAATCTCACTCGTGTACGACACAATCGACTTTAAAACATACGAGAAACTTGACACATTGTTGCACCGAGTTCCCAACACTGGAATGTGGGAGTGTGTTGACTTTTACCCGGTGTCTAAGACTGCGGGCAATGGGCTTGACACATCGGTCAATGGACCGGATGTGAAGCATATCGTGAAGGCTAGCATGGACGACACGAGGTTCGATCATTATGCTGTAGGCACGTATTTCGATTCAAACGGAACATGGATCCCCGATGATCCTACTATCGATGTTGGGATGACTGCCAGTTTAAGATATGATTACGGAAAGTTCTATGCTTCAAAGTCGTTTTACGACCAGAACAAGGGTCGAAGAGTCTTGTGGAGTTGGATTGGTGAGTCTGATAGTGAGGCTTCTGATGTACAAAAGGGTTGGTCTTCTCTCCAGGGTATCCCAAGAACCGTTGTCCTCGACACAAAGACAGGAAAGAACTTGGTTCAATGGCCAGTAGAAGAAATCAAATCTCTTAGACTAAGCAGCAAGCAATTTGATCTCGAGGTCGGTCCCGGGTCAGTGGTACCGGTCGATGTAGGTTCCGCAGCTCAGCTAGACATCGAAGCAGAATTCGAGATTAACAAAGAATCTCTAGACAAAATCATCGGAAACGCTTCGGTAGTGGCTGAAGCCGAGGAATTTAGCTGCGAAAAAAGCGGAGGCTCCACCGTCCGTGGTGCTTTAGGGCCATTCGGATTCTCGGTACTTGCCACAGAGAGCTTGTCTGAGCAAACACCGGTTTACTTCTATGTAGCTAAGGGAAAAGATTCAGAGCTCAAAACTTTCTTCTGCACAGACACCTCAAGGTCATCTGTTGCAAACGATGTCGTTAAACCGATATACGGTAGCGTCGTACCGGTTCTAAAAGGGGAGAAACTGACCATGAGAATTTTGGTGGATCATTCGATAGTAGAAGCATTCGGACAAGGTGGAAGAACATGTATAACATCAAGAGTCTATCCAACAACTGCAATCTATGGAGCAGCCAAGCTCTTCTTGTTCAATAATGCTCTTGATGCGACGGTTACGGCGTCGTTTACAGTTTGGCAAATGAACAGTGCTTTTATTCATCCTTACTCTGACGAAGCTGTCCGTGCTCTCTCCCGTACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0004553 GO:0004564 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0007275 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009739 GO:0009987 GO:0010033 GO:0016787 GO:0016798 GO:0022622 GO:0030312 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048731 GO:0048856 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071944 GO:0080022 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00052 ko00500 ko01100 map00052 map00500 map01100

Pfam Domains

Protein Families

DUF3357 Glyco_hydro_32C Glyco_hydro_32N

Protein Analysis

664

Amino Acids

73.84

Weight (kDa)

5.39

Isoelectric Point (pI)

34.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
INV_N	PF11837	21 - 117	1.6e-10	Beta-fructofuranosidase, N-terminal domain
Glyco_hydro_32N	PF00251	125 - 445	2.2e-102	Glycosyl hydrolases family 32 N-terminal domain
Glyco_hydro_32C	PF08244	449 - 644	3.6e-24	Glycosyl hydrolases family 32 C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12240 AT1G62660 AT1G62660 AT1G62660 AT1G62660
fragaria_vesca	FvH4_5g00290 FvH4_5g00290 FvH4_5g00290
malus_domestica	MD06G1066600.v1.1
prunus_persica	Prupe.5G075600_v2.0.a1 Prupe.5G075600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0202311
rosa_laevigata	RLG00000003612
rosa_multiflora	Rmu_sc0010249.1_g000001
rosa_roxburghii	Rroxscaffold_3G00254670
rosa_rugosa	Rorug07G0069800
rosa_samantha	Rh7AG196900 Rh7BG198200 Rh7CG208100 Rh7DG203600
rosa_wichuraiana	Rw7G017260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	244, 992, 1970
Acc36I	ACCTGC	1 cut(s)	646
Acc65I	GGTACC	2 cut(s)	435, 1405
AccB1I	GGYRCC	2 cut(s)	435, 1405
AciI	CCGC	7 cut(s)	33, 650, 746, 814, 971, 1425, 1539
AclWI	GGATC	9 cut(s)	201, 208, 230, 398, 824, 1090, 1100, 1103, 1788
AcoI	YGGCCR	2 cut(s)	78, 1331
AcsI	RAATTY	3 cut(s)	1451, 1520, 1770
AcuI	CTGAAG	2 cut(s)	699, 1530
AcvI	CACGTG	1 cut(s)	251
AcyI	GRCGYC	1 cut(s)	1913
AdeI	CACNNNGTG	1 cut(s)	502
AfaI	GTAC	9 cut(s)	437, 563, 741, 863, 1251, 1407, 1587, 1737, 1990
AfiI	CCNNNNNNNGG	8 cut(s)	128, 312, 507, 749, 763, 926, 1192, 1724
AflIII	ACRYGT	1 cut(s)	1820
AgeI	ACCGGT	4 cut(s)	632, 1408, 1618, 1738
AgsI	TTSAA	6 cut(s)	332, 706, 1083, 1170, 1328, 1885
AjiI	CACGTC	1 cut(s)	257
AjnI	CCWGG	1 cut(s)	1272
Alw21I	GWGCWC	4 cut(s)	11, 256, 1659, 1981
Alw26I	GTCTC	4 cut(s)	137, 148, 158, 230
Alw44I	GTGCAC	1 cut(s)	252
AlwI	GGATC	9 cut(s)	201, 208, 230, 398, 824, 1090, 1100, 1103, 1788
Ama87I	CYCGRG	3 cut(s)	721, 1382, 1394
AoxI	GGCC	4 cut(s)	78, 103, 1331, 1568
ApaLI	GTGCAC	1 cut(s)	252
ApeKI	GCWGC	5 cut(s)	467, 1365, 1427, 1527, 1865
ApoI	RAATTY	3 cut(s)	1451, 1520, 1770
ArsI	GACNNNNNNTTYG	2 cut(s)	1031, 1063
AsiGI	ACCGGT	4 cut(s)	632, 1408, 1618, 1738
Asp700I	GAANNNNTTC	2 cut(s)	1495, 1648
Asp718I	GGTACC	2 cut(s)	435, 1405
AspS9I	GGNCC	9 cut(s)	104, 413, 511, 526, 582, 658, 1001, 1391, 1568
AsuC2I	CCSGG	8 cut(s)	109, 722, 723, 731, 750, 956, 1395, 1396
AsuHPI	GGTGA	3 cut(s)	320, 491, 1235
AsuNHI	GCTAGC	1 cut(s)	1028
AvaI	CYCGRG	3 cut(s)	721, 1382, 1394
AvaII	GGWCC	7 cut(s)	413, 511, 526, 582, 658, 1001, 1391
AxyI	CCTNAGG	1 cut(s)	762
BaeGI	GKGCMC	1 cut(s)	256
BalI	TGGCCA	1 cut(s)	1333
BamHI	GGATCC	1 cut(s)	1095
BanI	GGYRCC	2 cut(s)	435, 1405
BanII	GRGCYC	2 cut(s)	11, 1659
BarI	GAAGNNNNNNTAC	2 cut(s)	428, 460
BauI	CACGAG	3 cut(s)	64, 857, 1042
BbrPI	CACGTG	1 cut(s)	251
BbsI	GAAGAC	4 cut(s)	124, 687, 800, 1257
Bbv12I	GWGCWC	4 cut(s)	11, 256, 1659, 1981
BbvI	GCAGC	5 cut(s)	479, 1377, 1439, 1514, 1877
BccI	CCATC	3 cut(s)	281, 426, 602
BceAI	ACGGC	5 cut(s)	125, 381, 455, 485, 1926
BcgI	CGANNNNNNTGC	4 cut(s)	1040, 1057, 1074, 1091
BciT130I	CCWGG	1 cut(s)	1274
BciVI	GTATCC	2 cut(s)	764, 1289
BclI	TGATCA	1 cut(s)	553
BcnI	CCSGG	8 cut(s)	109, 722, 723, 731, 750, 956, 1395, 1396
BcoDI	GTCTC	4 cut(s)	137, 148, 158, 230
BfaI	CTAG	6 cut(s)	204, 507, 737, 1029, 1436, 1475
BfmI	CTRYAG	2 cut(s)	615, 1062
BfuAI	ACCTGC	1 cut(s)	646
BfuI	GTATCC	2 cut(s)	764, 1289
BisI	GCNGC	5 cut(s)	468, 1366, 1428, 1528, 1866
BlpI	GCTNAGC	2 cut(s)	589, 1431
BlsI	GCNGC	5 cut(s)	469, 1367, 1429, 1529, 1867
Bme1390I	CCNGG	9 cut(s)	109, 722, 723, 731, 750, 956, 1274, 1395, 1396
Bme18I	GGWCC	7 cut(s)	413, 511, 526, 582, 658, 1001, 1391
BmeT110I	CYCGRG	3 cut(s)	721, 1382, 1394
BmgBI	CACGTC	1 cut(s)	257
BmgT120I	GGNCC	9 cut(s)	104, 413, 511, 526, 582, 658, 1001, 1391, 1568
BmrFI	CCNGG	9 cut(s)	109, 722, 723, 731, 750, 956, 1274, 1395, 1396
BmsI	GCATC	2 cut(s)	4, 1888
BmtI	GCTAGC	1 cut(s)	1032
BpiI	GAAGAC	4 cut(s)	124, 687, 800, 1257
BpmI	CTGGAG	1 cut(s)	1256
Bpu10I	CCTNAGC	2 cut(s)	384, 1638
Bpu1102I	GCTNAGC	2 cut(s)	589, 1431
BpuEI	CTTGAG	1 cut(s)	1669
BpuMI	CCSGG	8 cut(s)	109, 722, 723, 731, 750, 956, 1395, 1396
Bsa29I	ATCGAT	2 cut(s)	225, 1116
BsaAI	YACGTR	2 cut(s)	251, 1071
BsaBI	GATNNNNATC	1 cut(s)	205
BsaHI	GRCGYC	1 cut(s)	1913
BsaI	GGTCTC	2 cut(s)	137, 158
BsaJI	CCNNGG	9 cut(s)	100, 107, 543, 721, 722, 1273, 1394, 1515, 1555
BsaWI	WCCGGW	6 cut(s)	305, 632, 1003, 1408, 1618, 1738
Bsc4I	CCNNNNNNNGG	8 cut(s)	128, 312, 507, 749, 763, 926, 1192, 1724
Bse118I	RCCGGY	6 cut(s)	322, 584, 632, 1408, 1618, 1738
Bse1I	ACTGG	4 cut(s)	850, 931, 1134, 1334
Bse21I	CCTNAGG	1 cut(s)	762
Bse3DI	GCAATG	1 cut(s)	982
Bse8I	GATNNNNATC	1 cut(s)	205
BseBI	CCWGG	1 cut(s)	1274
BseCI	ATCGAT	2 cut(s)	225, 1116
BseDI	CCNNGG	9 cut(s)	100, 107, 543, 721, 722, 1273, 1394, 1515, 1555
BseGI	GGATG	5 cut(s)	402, 437, 1012, 1131, 1951
BseJI	GATNNNNATC	1 cut(s)	205
BseLI	CCNNNNNNNGG	8 cut(s)	128, 312, 507, 749, 763, 926, 1192, 1724
BseMI	GCAATG	1 cut(s)	982
BseMII	CTCAG	4 cut(s)	375, 603, 1445, 1599
BseNI	ACTGG	4 cut(s)	850, 931, 1134, 1334
BseRI	GAGGAG	1 cut(s)	125
BseSI	GKGCMC	1 cut(s)	256
BseXI	GCAGC	5 cut(s)	479, 1377, 1439, 1514, 1877
BsgI	GTGCAG	2 cut(s)	674, 1657
Bsh1285I	CGRYCG	1 cut(s)	1413
BshFI	GGCC	4 cut(s)	80, 105, 1333, 1570
BshNI	GGYRCC	2 cut(s)	435, 1405
BshTI	ACCGGT	4 cut(s)	632, 1408, 1618, 1738
BshVI	ATCGAT	2 cut(s)	225, 1116
BsiEI	CGRYCG	1 cut(s)	1413
BsiHKAI	GWGCWC	4 cut(s)	11, 256, 1659, 1981
BsiHKCI	CYCGRG	3 cut(s)	721, 1382, 1394
BslFI	GGGAC	2 cut(s)	524, 1377
BslI	CCNNNNNNNGG	8 cut(s)	128, 312, 507, 749, 763, 926, 1192, 1724
BsmAI	GTCTC	4 cut(s)	137, 148, 158, 230
BsmBI	CGTCTC	2 cut(s)	148, 230
BsmFI	GGGAC	2 cut(s)	524, 1377
BsmI	GAATGC	1 cut(s)	1799
BsnI	GGCC	4 cut(s)	80, 105, 1333, 1570
Bso31I	GGTCTC	2 cut(s)	137, 158
BsoBI	CYCGRG	3 cut(s)	721, 1382, 1394
Bsp1286I	GDGCHC	4 cut(s)	11, 256, 1659, 1981
Bsp1407I	TGTACA	1 cut(s)	1249
Bsp1720I	GCTNAGC	2 cut(s)	589, 1431
Bsp19I	CCATGG	2 cut(s)	100, 543
BspACI	CCGC	7 cut(s)	33, 650, 746, 814, 971, 1425, 1539
BspANI	GGCC	4 cut(s)	80, 105, 1333, 1570
BspCNI	CTCAG	4 cut(s)	376, 602, 1444, 1600
BspDI	ATCGAT	2 cut(s)	225, 1116
BspMI	ACCTGC	1 cut(s)	646
BspOI	GCTAGC	1 cut(s)	1032
BspPI	GGATC	9 cut(s)	201, 208, 230, 398, 824, 1090, 1100, 1103, 1788
BspQI	GCTCTTC	1 cut(s)	1880
BspT107I	GGYRCC	2 cut(s)	435, 1405
BspTNI	GGTCTC	2 cut(s)	137, 158
BsrDI	GCAATG	1 cut(s)	982
BsrFI	RCCGGY	6 cut(s)	322, 584, 632, 1408, 1618, 1738
BsrGI	TGTACA	1 cut(s)	1249
BsrI	ACTGG	4 cut(s)	850, 931, 1134, 1334
BssAI	RCCGGY	6 cut(s)	322, 584, 632, 1408, 1618, 1738
BssECI	CCNNGG	9 cut(s)	100, 107, 543, 721, 722, 1273, 1394, 1515, 1555
BssNI	GRCGYC	1 cut(s)	1913
BssSI	CACGAG	3 cut(s)	64, 857, 1042
BssT1I	CCWWGG	2 cut(s)	100, 543
Bst2BI	CACGAG	3 cut(s)	64, 857, 1042
Bst2UI	CCWGG	1 cut(s)	1274
Bst4CI	ACNGT	8 cut(s)	413, 575, 1291, 1552, 1727, 1906, 1924, 1940
Bst6I	CTCTTC	2 cut(s)	1195, 1880
BstACI	GRCGYC	1 cut(s)	1913
BstAPI	GCANNNNNTGC	1 cut(s)	260
BstAUI	TGTACA	1 cut(s)	1249
BstBAI	YACGTR	2 cut(s)	251, 1071
BstC8I	GCNNGC	5 cut(s)	7, 324, 973, 1030, 1370
BstDEI	CTNAG	9 cut(s)	384, 589, 762, 963, 1355, 1361, 1431, 1608, 1638
BstDSI	CCRYGG	3 cut(s)	100, 543, 1555
BstENI	CCTNNNNNAGG	1 cut(s)	761
BstF5I	GGATG	5 cut(s)	402, 437, 1012, 1131, 1951
BstMAI	GTCTC	4 cut(s)	137, 148, 158, 230
BstMCI	CGRYCG	1 cut(s)	1413
BstMWI	GCNNNNNNNGC	6 cut(s)	185, 260, 548, 1536, 1898, 1976
BstNI	CCWGG	1 cut(s)	1274
BstNSI	RCATGY	1 cut(s)	1824
BstSCI	CCNGG	9 cut(s)	107, 720, 721, 729, 748, 954, 1272, 1393, 1394
BstSFI	CTRYAG	2 cut(s)	615, 1062
BstSLI	GKGCMC	1 cut(s)	256
BstV1I	GCAGC	5 cut(s)	479, 1377, 1439, 1514, 1877
BstV2I	GAAGAC	4 cut(s)	124, 687, 800, 1257
BstX2I	RGATCY	3 cut(s)	200, 206, 1095
BstXI	CCANNNNNNTGG	1 cut(s)	544
BstYI	RGATCY	3 cut(s)	200, 206, 1095
Bsu15I	ATCGAT	2 cut(s)	225, 1116
Bsu36I	CCTNAGG	1 cut(s)	762
BsuI	GTATCC	2 cut(s)	764, 1289
BsuRI	GGCC	4 cut(s)	80, 105, 1333, 1570
BsuTUI	ATCGAT	2 cut(s)	225, 1116
BtgI	CCRYGG	3 cut(s)	100, 543, 1555
BtgZI	GCGATG	2 cut(s)	148, 169
BtrI	CACGTC	1 cut(s)	257
BtsCI	GGATG	5 cut(s)	402, 437, 1012, 1131, 1951
BtsIMutI	CAGTG	3 cut(s)	924, 1407, 1945
BveI	ACCTGC	1 cut(s)	646
Cac8I	GCNNGC	5 cut(s)	7, 324, 973, 1030, 1370
Cfr10I	RCCGGY	6 cut(s)	322, 584, 632, 1408, 1618, 1738
Cfr13I	GGNCC	9 cut(s)	104, 413, 511, 526, 582, 658, 1001, 1391, 1568
Cfr9I	CCCGGG	2 cut(s)	721, 1394
ClaI	ATCGAT	2 cut(s)	225, 1116
CseI	GACGC	2 cut(s)	1720, 1902
Csp6I	GTAC	9 cut(s)	436, 562, 740, 862, 1250, 1406, 1586, 1736, 1989
CspAI	ACCGGT	4 cut(s)	632, 1408, 1618, 1738
CspCI	CAANNNNNGTGG	2 cut(s)	684, 719
CviAII	CATG	8 cut(s)	101, 544, 622, 789, 1033, 1092, 1765, 1821
CviQI	GTAC	9 cut(s)	436, 562, 740, 862, 1250, 1406, 1586, 1736, 1989
DdeI	CTNAG	9 cut(s)	384, 589, 762, 963, 1355, 1361, 1431, 1608, 1638
DraI	TTTAAA	2 cut(s)	127, 880
DraIII	CACNNNGTG	1 cut(s)	502
DrdI	GACNNNNNNGTC	3 cut(s)	244, 992, 1970
DseDI	GACNNNNNNGTC	3 cut(s)	244, 992, 1970
EaeI	YGGCCR	2 cut(s)	78, 1331
Eam1104I	CTCTTC	2 cut(s)	1195, 1880
EarI	CTCTTC	2 cut(s)	1195, 1880
EciI	GGCGGA	3 cut(s)	22, 735, 829
Ecl136II	GAGCTC	2 cut(s)	9, 1657
Eco130I	CCWWGG	2 cut(s)	100, 543
Eco24I	GRGCYC	2 cut(s)	11, 1659
Eco31I	GGTCTC	2 cut(s)	137, 158
Eco47I	GGWCC	7 cut(s)	413, 511, 526, 582, 658, 1001, 1391
Eco53kI	GAGCTC	2 cut(s)	9, 1657
Eco57I	CTGAAG	2 cut(s)	699, 1530
Eco72I	CACGTG	1 cut(s)	251
Eco81I	CCTNAGG	1 cut(s)	762
Eco88I	CYCGRG	3 cut(s)	721, 1382, 1394
EcoICRI	GAGCTC	2 cut(s)	9, 1657
EcoNI	CCTNNNNNAGG	1 cut(s)	761
EcoO109I	RGGNCCY	1 cut(s)	511
EcoRI	GAATTC	1 cut(s)	1451
EcoRII	CCWGG	1 cut(s)	1272
EcoT14I	CCWWGG	2 cut(s)	100, 543
EcoT38I	GRGCYC	2 cut(s)	11, 1659
ErhI	CCWWGG	2 cut(s)	100, 543
Esp3I	CGTCTC	2 cut(s)	148, 230
FaeI	CATG	8 cut(s)	104, 547, 625, 792, 1036, 1095, 1768, 1824
FalI	AAGNNNNNCTT	2 cut(s)	1862, 1894
FaqI	GGGAC	2 cut(s)	524, 1377
FatI	CATG	8 cut(s)	100, 543, 621, 788, 1032, 1091, 1764, 1820
FauI	CCCGC	1 cut(s)	964
FbaI	TGATCA	1 cut(s)	553
Fnu4HI	GCNGC	5 cut(s)	468, 1366, 1428, 1528, 1866
FokI	GGATG	5 cut(s)	409, 444, 1019, 1138, 1938
FriOI	GRGCYC	2 cut(s)	11, 1659
Fsp4HI	GCNGC	5 cut(s)	468, 1366, 1428, 1528, 1866
FspBI	CTAG	6 cut(s)	204, 507, 737, 1029, 1436, 1475
GluI	GCNGC	5 cut(s)	468, 1366, 1428, 1528, 1866
GsuI	CTGGAG	1 cut(s)	1256
HaeIII	GGCC	4 cut(s)	80, 105, 1333, 1570
HgaI	GACGC	2 cut(s)	1720, 1902
Hin1I	GRCGYC	1 cut(s)	1913
Hin1II	CATG	8 cut(s)	104, 547, 625, 792, 1036, 1095, 1768, 1824
HincII	GTYRAC	1 cut(s)	946
HindII	GTYRAC	1 cut(s)	946
HphI	GGTGA	3 cut(s)	320, 491, 1235
Hpy166II	GTNNAC	6 cut(s)	254, 582, 862, 946, 1624, 1920
Hpy188III	TCNNGA	9 cut(s)	92, 204, 820, 1021, 1382, 1456, 1475, 1833, 1895
Hpy8I	GTNNAC	6 cut(s)	254, 582, 862, 946, 1624, 1920
Hpy99I	CGWCG	6 cut(s)	179, 200, 218, 1736, 1906, 1918
HpyAV	CCTTC	3 cut(s)	212, 797, 1018
HpyCH4III	ACNGT	8 cut(s)	413, 575, 1291, 1552, 1727, 1906, 1924, 1940
HpyCH4IV	ACGT	6 cut(s)	174, 245, 250, 256, 280, 1070
HpyCH4V	TGCA	7 cut(s)	254, 655, 788, 910, 1674, 1700, 1853
HpyF10VI	GCNNNNNNNGC	6 cut(s)	185, 260, 548, 1536, 1898, 1976
HpyF3I	CTNAG	9 cut(s)	384, 589, 762, 963, 1355, 1361, 1431, 1608, 1638
HpySE526I	ACGT	6 cut(s)	174, 245, 250, 256, 280, 1070
Hsp92I	GRCGYC	1 cut(s)	1913
Hsp92II	CATG	8 cut(s)	104, 547, 625, 792, 1036, 1095, 1768, 1824
KpnI	GGTACC	2 cut(s)	439, 1409
KroI	GCCGGC	1 cut(s)	322
KroNI	GCCGGC	1 cut(s)	324
Ksp22I	TGATCA	1 cut(s)	553
LguI	GCTCTTC	1 cut(s)	1880
LmnI	GCTCC	3 cut(s)	14, 1550, 1862
Lsp1109I	GCAGC	5 cut(s)	479, 1377, 1439, 1514, 1877
LweI	GCATC	2 cut(s)	4, 1888
MaeI	CTAG	6 cut(s)	204, 507, 737, 1029, 1436, 1475
MaeII	ACGT	6 cut(s)	174, 245, 250, 256, 280, 1070
MaeIII	GTNAC	6 cut(s)	246, 281, 479, 494, 773, 1906
MflI	RGATCY	3 cut(s)	200, 206, 1095
MhlI	GDGCHC	4 cut(s)	11, 256, 1659, 1981
MlsI	TGGCCA	1 cut(s)	1333
MluCI	AATT	5 cut(s)	391, 1373, 1451, 1520, 1770
MluNI	TGGCCA	1 cut(s)	1333
MlyI	GAGTC	4 cut(s)	560, 1212, 1235, 1845
MmeI	TCCRAC	5 cut(s)	234, 252, 594, 1196, 1868
Mox20I	TGGCCA	1 cut(s)	1333
MreI	CGCCGGCG	1 cut(s)	322
MroNI	GCCGGC	1 cut(s)	322
MroXI	GAANNNNTTC	2 cut(s)	1495, 1648
MscI	TGGCCA	1 cut(s)	1333
MseI	TTAA	5 cut(s)	126, 879, 1139, 1461, 1713
MslI	CAYNNNNRTG	2 cut(s)	542, 929
Msp20I	TGGCCA	1 cut(s)	1333
MspR9I	CCNGG	9 cut(s)	109, 722, 723, 731, 750, 956, 1274, 1395, 1396
Mva1269I	GAATGC	1 cut(s)	1799
MvaI	CCWGG	1 cut(s)	1274
MwoI	GCNNNNNNNGC	6 cut(s)	185, 260, 548, 1536, 1898, 1976
NaeI	GCCGGC	1 cut(s)	324
NciI	CCSGG	8 cut(s)	109, 722, 723, 731, 750, 956, 1395, 1396
NcoI	CCATGG	2 cut(s)	100, 543
NgoMIV	GCCGGC	1 cut(s)	322
NheI	GCTAGC	1 cut(s)	1028
NlaIII	CATG	8 cut(s)	104, 547, 625, 792, 1036, 1095, 1768, 1824
NmeAIII	GCCGAG	1 cut(s)	1540
NmuCI	GTSAC	4 cut(s)	246, 479, 494, 773
NspI	RCATGY	1 cut(s)	1824
PaeR7I	CTCGAG	1 cut(s)	1382
PciI	ACATGT	1 cut(s)	1820
PciSI	GCTCTTC	1 cut(s)	1880
PcsI	WCGNNNNNNNCGW	6 cut(s)	171, 192, 222, 870, 1731, 1910
PctI	GAATGC	1 cut(s)	1799
PdiI	GCCGGC	1 cut(s)	324
PdmI	GAANNNNTTC	2 cut(s)	1495, 1648
PfeI	GAWTC	6 cut(s)	88, 849, 1079, 1469, 1578, 1649
PinAI	ACCGGT	4 cut(s)	632, 1408, 1618, 1738
PkrI	GCNGC	5 cut(s)	469, 1367, 1429, 1529, 1867
PleI	GAGTC	4 cut(s)	560, 1211, 1234, 1844
PmaCI	CACGTG	1 cut(s)	251
PmlI	CACGTG	1 cut(s)	251
PpsI	GAGTC	4 cut(s)	560, 1211, 1234, 1844
Ppu21I	YACGTR	2 cut(s)	251, 1071
PpuMI	RGGWCCY	1 cut(s)	511
PscI	ACATGT	1 cut(s)	1820
Psp124BI	GAGCTC	2 cut(s)	11, 1659
Psp5II	RGGWCCY	1 cut(s)	511
Psp6I	CCWGG	1 cut(s)	1272
PspCI	CACGTG	1 cut(s)	251
PspGI	CCWGG	1 cut(s)	1272
PspPI	GGNCC	9 cut(s)	104, 413, 511, 526, 582, 658, 1001, 1391, 1568
PspPPI	RGGWCCY	1 cut(s)	511
PsuI	RGATCY	3 cut(s)	200, 206, 1095
RsaI	GTAC	9 cut(s)	437, 563, 741, 863, 1251, 1407, 1587, 1737, 1990
RsaNI	GTAC	9 cut(s)	436, 562, 740, 862, 1250, 1406, 1586, 1736, 1989
RseI	CAYNNNNRTG	2 cut(s)	542, 929
SacI	GAGCTC	2 cut(s)	11, 1659
SapI	GCTCTTC	1 cut(s)	1880
SaqAI	TTAA	5 cut(s)	126, 879, 1139, 1461, 1713
SatI	GCNGC	5 cut(s)	468, 1366, 1428, 1528, 1866
Sau96I	GGNCC	9 cut(s)	104, 413, 511, 526, 582, 658, 1001, 1391, 1568
SchI	GAGTC	4 cut(s)	560, 1212, 1235, 1845
ScrFI	CCNGG	9 cut(s)	109, 722, 723, 731, 750, 956, 1274, 1395, 1396
SduI	GDGCHC	4 cut(s)	11, 256, 1659, 1981
SfaNI	GCATC	2 cut(s)	4, 1888
SfcI	CTRYAG	2 cut(s)	615, 1062
Sfr274I	CTCGAG	1 cut(s)	1382
SgrAI	CRCCGGYG	1 cut(s)	322
SinI	GGWCC	7 cut(s)	413, 511, 526, 582, 658, 1001, 1391
SlaI	CTCGAG	1 cut(s)	1382
SmaI	CCCGGG	2 cut(s)	723, 1396
SmiMI	CAYNNNNRTG	2 cut(s)	542, 929
SmlI	CTYRAG	2 cut(s)	1382, 1684
SmoI	CTYRAG	2 cut(s)	1382, 1684
Sse9I	AATT	5 cut(s)	391, 1373, 1451, 1520, 1770
SsiI	CCGC	7 cut(s)	33, 650, 746, 814, 971, 1425, 1539
SspMI	CTAG	6 cut(s)	204, 507, 737, 1029, 1436, 1475
SstI	GAGCTC	2 cut(s)	11, 1659
StyD4I	CCNGG	9 cut(s)	107, 720, 721, 729, 748, 954, 1272, 1393, 1394
StyI	CCWWGG	2 cut(s)	100, 543
TaaI	ACNGT	8 cut(s)	413, 575, 1291, 1552, 1727, 1906, 1924, 1940
TaiI	ACGT	6 cut(s)	177, 248, 253, 259, 283, 1073
TaqII	GACCGA	2 cut(s)	982, 1379
TasI	AATT	5 cut(s)	391, 1373, 1451, 1520, 1770
TatI	WGTACW	1 cut(s)	1249
TfiI	GAWTC	6 cut(s)	88, 849, 1079, 1469, 1578, 1649
Tru1I	TTAA	5 cut(s)	126, 879, 1139, 1461, 1713
Tru9I	TTAA	5 cut(s)	126, 879, 1139, 1461, 1713
TscAI	CASTG	3 cut(s)	931, 1407, 1945
TseFI	GTSAC	4 cut(s)	246, 479, 494, 773
TseI	GCWGC	5 cut(s)	467, 1365, 1427, 1527, 1865
Tsp45I	GTSAC	4 cut(s)	246, 479, 494, 773
TspDTI	ATGAA	6 cut(s)	151, 243, 362, 413, 1940, 1949
TspGWI	ACGGA	6 cut(s)	213, 761, 1101, 1167, 1544, 1964
TspMI	CCCGGG	2 cut(s)	721, 1394
TspRI	CASTG	3 cut(s)	931, 1407, 1945
VneI	GTGCAC	1 cut(s)	252
VpaK11BI	GGWCC	7 cut(s)	413, 511, 526, 582, 658, 1001, 1391
XagI	CCTNNNNNAGG	1 cut(s)	761
XapI	RAATTY	3 cut(s)	1451, 1520, 1770
XbaI	TCTAGA	2 cut(s)	203, 1474
XceI	RCATGY	1 cut(s)	1824
XcmI	CCANNNNNNNNNTGG	2 cut(s)	786, 1771
XhoI	CTCGAG	1 cut(s)	1382
XmaI	CCCGGG	2 cut(s)	721, 1394
XmnI	GAANNNNTTC	2 cut(s)	1495, 1648
XspI	CTAG	6 cut(s)	204, 507, 737, 1029, 1436, 1475

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.