Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0010222

Physical Location & Seq

Genomic Coordinates Reverse (-)

14217015 .. 14222078

Gene Structure 5064 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1066600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2037 bp

ATGGCAGACCCTAGACCTTTTCTTCCATCGGATCCCGATGCCCTGCCGTACACCTACACTCCTCTCCCGAATCCGCTCGAAGCTTCGGAAATCCGAAAGAGGCAGTTTAAGGAGCTCCTAGCAGTCTTTTCTGGGTTGCTGATGCTTTCTTTGTTGGTGGTCATTATTAGTAGTGGCAACAATTCTGATTCCCATGCAAATAAAAATGAGCCAGTGTTTTTGGCTAAAGAGCCTTATACAACAAGAACCGTCAAACTGCGCCCGGTTCCTCGGGGCATAGCAGCCGGAGTGTCCGAGAAGTCGAATCGCTTGGCCGATGCTGTGGACGGGCCGGCATTTCCATGGAACAATAGCATGCTGTCATGGCAGAGAACTGCTTTCCATTTCCAGCCTGAGAAGAACTGGATGAATGGTCCATTATTCTACAAAGGTTGGTACCACTTTTTCTACCAGTGGAATCCCAATGGTGCAATATGGGGAGACATTGTATGGGGCCATGCAGTGTCCAAGGACTTGATCCATTGGCTTCACCTCCCTCTAGCCATGGTGGCTGACCAGTGGTACGACATCAATGGAGTCTGGACTGGCTCAGCCACCATTCTCCCTGATGGCAAAATTGTCATGCTCTACACTGGCTCAACCAATGAGTCGGTCCAAGTCCAAAACCTTGCATATCCTGCTGACCACAATGACCCCCTCCTCACCATATGGGTCAAGTACTCCGGCAACCCGATTCTAGTCCCGCCCCCGGGCATCGGATACAAGGACTTCCGTGACCCGACAACCGCTTGGTACACGTCTCAAGGAAAATGGCGCATTACTATTGGGTCAAAGCTTAACAAAACAGGCATATCCTTGGTTTATGATACCAAGGACTTCAAAACCTATGAGCAATTGAATGGGGTGCTTCATGCTGTCCCTGGGACTGGAATGTGGGAGTGTGTGGATTTTTATCCGGTGTCGAAAACCAGTGACAAAGGACTGGATACTTCGGTGAATGGGCCTGATGTGAAGCATGTGGTGAAAGCTAGTCTTGATGATGATAGGAATGATTACTATTCATTGGGGACCTATGAGGAGAAGACAGGCAAATGGGTTCCTGATAACCAAAAGATTGATGTTGGTATTGGAATTAGGTATGATTATGGCATTTTCTATGCCTCCAAGACTTTCTATGACCAAAACAAGGAGAGGAGAGTTTTGTGGGGTTGGATTGGAGAGTCTGACAGTGAAAATGCTGATATGCAGAAGGGATGGGCCTCTGTTCAGGGAATTCCAAGGACAGTGGTATTTGATAAGAAGACTGGAAGCAATCTACTTCAATGGCCAGTGGAGGAGGTAGAGAAATTGAGATTGAGCATTACGGACTTCGACAAGGTTGAGGTCAAGGCAGGCTCAGTGCTGCCGCTTCAAGTTGGCACAGCTACACAGTTGGACATTGTTGCCGAGTTCGAGTTGGACAAGAAAGTTTTGGAGAGCGTAGCTGAATCTAATGAGGTGTTCAGTTGCAATACCAGCGCTGGAGCTTCCCATCGTGGTGCTTTAGGACCCTTTGGTCTTCTTGTTCTTGCAGATGAGACCCTCTCAGAGCAGACTCCTGTCTACTTTTACGTAGCCAAAGGACCTGGTGGCAATTTTGATACTTTCTTCTGCGCCGATCAAACAAGGTCTTCTGTGGCTAATGATGTTAAGAAAAAAGTTACTGGCAGCTATGTTCCAGTTCTAAAAGATGAAAAATTATCAGCGAGGATTCTGGTTGACCATTCGATAGTCGAAAGCTTTGCTCAAGGTGGAAGAACAACCATCACATCTAGGGTTTACCCAACCAAGGCAATTTATGGGGCTGCTAGGCTAATTTTGTTCAACAATGCTACCGAGACCAGTGTTACTGCCTCCCTCAAAGTATGGCAGATGAACTCTGCATTTATCCGCCCGTACCATCCGGACGAAACGAGCAACGCAAGCTTAAACCTTGTTACCTACATCAAATTCATTGTCACATTCTTTATTATTTTTACTTTATCTTTTCTTAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0004553 GO:0004564 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0007275 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009739 GO:0009987 GO:0010033 GO:0016787 GO:0016798 GO:0022622 GO:0030312 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048731 GO:0048856 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071944 GO:0080022 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00052 ko00500 ko01100 map00052 map00500 map01100

Pfam Domains

Protein Families

DUF3357 Glyco_hydro_32C Glyco_hydro_32N

Protein Analysis

679

Amino Acids

75.82

Weight (kDa)

6.11

Isoelectric Point (pI)

24.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
INV_N	PF11837	19 - 120	1.8e-17	Beta-fructofuranosidase, N-terminal domain
Glyco_hydro_32N	PF00251	128 - 443	9.7e-97	Glycosyl hydrolases family 32 N-terminal domain
Glyco_hydro_32C	PF08244	446 - 637	1.8e-23	Glycosyl hydrolases family 32 C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12240 AT1G62660 AT1G62660 AT1G62660 AT1G62660
fragaria_vesca	FvH4_5g00290 FvH4_5g00290 FvH4_5g00290
malus_domestica	MD06G1066600.v1.1
prunus_persica	Prupe.5G075600_v2.0.a1 Prupe.5G075600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0202311
rosa_laevigata	RLG00000003612
rosa_multiflora	Rmu_sc0010249.1_g000001
rosa_roxburghii	Rroxscaffold_3G00254670
rosa_rugosa	Rorug07G0069800
rosa_samantha	Rh7AG196900 Rh7BG198200 Rh7CG208100 Rh7DG203600
rosa_wichuraiana	Rw7G017260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1554
Acc65I	GGTACC	1 cut(s)	435
AccB1I	GGYRCC	1 cut(s)	435
AccBSI	CCGCTC	1 cut(s)	76
AccI	GTMKAC	1 cut(s)	1602
AccIII	TCCGGA	1 cut(s)	1942
AciI	CCGC	5 cut(s)	74, 743, 786, 1406, 1930
AclWI	GGATC	3 cut(s)	26, 39, 511
AcoI	YGGCCR	2 cut(s)	312, 1325
AcsI	RAATTY	2 cut(s)	1272, 1988
AfaI	GTAC	6 cut(s)	50, 437, 563, 719, 794, 1937
AfeI	AGCGCT	1 cut(s)	1519
AfiI	CCNNNNNNNGG	6 cut(s)	748, 749, 755, 926, 1186, 1828
AflIII	ACRYGT	1 cut(s)	795
AgsI	TTSAA	5 cut(s)	880, 898, 1322, 1412, 1864
AjiI	CACGTC	1 cut(s)	798
AjnI	CCWGG	2 cut(s)	919, 1624
Alw21I	GWGCWC	1 cut(s)	117
Alw26I	GTCTC	4 cut(s)	474, 804, 1571, 1871
AlwI	GGATC	3 cut(s)	26, 39, 511
Ama87I	CYCGRG	2 cut(s)	270, 748
Aor13HI	TCCGGA	1 cut(s)	1942
Aor51HI	AGCGCT	1 cut(s)	1519
AoxI	GGCC	6 cut(s)	312, 329, 493, 1001, 1257, 1325
ApeKI	GCWGC	4 cut(s)	281, 1402, 1707, 1844
ApoI	RAATTY	2 cut(s)	1272, 1988
ArsI	GACNNNNNNTTYG	2 cut(s)	1980, 2012
Asp718I	GGTACC	1 cut(s)	435
AspLEI	GCGC	4 cut(s)	261, 816, 1520, 1655
AspS9I	GGNCC	9 cut(s)	329, 413, 493, 652, 1001, 1068, 1257, 1547, 1622
AsuC2I	CCSGG	3 cut(s)	263, 749, 750
AsuHPI	GGTGA	4 cut(s)	521, 694, 1006, 1033
AvaI	CYCGRG	2 cut(s)	270, 748
AvaII	GGWCC	5 cut(s)	413, 652, 1068, 1547, 1622
BaeI	ACNNNNGTAYC	2 cut(s)	978, 1011
BalI	TGGCCA	1 cut(s)	1327
BamHI	GGATCC	1 cut(s)	31
BanI	GGYRCC	1 cut(s)	435
BanII	GRGCYC	1 cut(s)	117
BbsI	GAAGAC	4 cut(s)	1088, 1307, 1550, 1662
Bbv12I	GWGCWC	1 cut(s)	117
BbvI	GCAGC	4 cut(s)	293, 1389, 1719, 1831
BccI	CCATC	6 cut(s)	34, 602, 1248, 1539, 1811, 1947
BceAI	ACGGC	1 cut(s)	31
BcgI	CGANNNNNNTGC	2 cut(s)	1763, 1797
BciT130I	CCWGG	2 cut(s)	921, 1626
BciVI	GTATCC	2 cut(s)	752, 979
BcnI	CCSGG	3 cut(s)	263, 749, 750
BcoDI	GTCTC	4 cut(s)	474, 804, 1571, 1871
BfaI	CTAG	7 cut(s)	12, 119, 539, 737, 1029, 1812, 1848
BfoI	RGCGCY	1 cut(s)	1521
BfuI	GTATCC	2 cut(s)	752, 979
BglI	GCCNNNNNGGC	1 cut(s)	548
BisI	GCNGC	5 cut(s)	282, 1403, 1406, 1708, 1845
BlpI	GCTNAGC	1 cut(s)	589
BlsI	GCNGC	5 cut(s)	283, 1404, 1407, 1709, 1846
BmcAI	AGTACT	1 cut(s)	719
Bme1390I	CCNGG	5 cut(s)	263, 749, 750, 921, 1626
Bme18I	GGWCC	5 cut(s)	413, 652, 1068, 1547, 1622
BmeT110I	CYCGRG	2 cut(s)	270, 748
BmgBI	CACGTC	1 cut(s)	798
BmgT120I	GGNCC	9 cut(s)	329, 413, 493, 652, 1001, 1068, 1257, 1547, 1622
BmiI	GGNNCC	7 cut(s)	33, 267, 437, 494, 1069, 1098, 1549
BmrFI	CCNGG	5 cut(s)	263, 749, 750, 921, 1626
BmsI	GCATC	4 cut(s)	28, 132, 307, 762
BoxI	GACNNNNGTC	1 cut(s)	1598
BpiI	GAAGAC	4 cut(s)	1088, 1307, 1550, 1662
BplI	GAGNNNNNCTC	2 cut(s)	1568, 1600
BpmI	CTGGAG	1 cut(s)	1542
Bpu1102I	GCTNAGC	1 cut(s)	589
BpuEI	CTTGAG	2 cut(s)	786, 1770
BpuMI	CCSGG	3 cut(s)	263, 749, 750
BsaAI	YACGTR	1 cut(s)	1612
BsaBI	GATNNNNATC	1 cut(s)	951
BsaI	GGTCTC	2 cut(s)	1571, 1871
BsaWI	WCCGGW	2 cut(s)	955, 1942
BsaXI	ACNNNNNCTCC	4 cut(s)	43, 73, 471, 501
Bsc4I	CCNNNNNNNGG	6 cut(s)	748, 749, 755, 926, 1186, 1828
Bse118I	RCCGGY	1 cut(s)	331
Bse8I	GATNNNNATC	1 cut(s)	951
BseAI	TCCGGA	1 cut(s)	1942
BseBI	CCWGG	2 cut(s)	921, 1626
BseGI	GGATG	3 cut(s)	411, 1259, 1939
BseJI	GATNNNNATC	1 cut(s)	951
BseLI	CCNNNNNNNGG	6 cut(s)	748, 749, 755, 926, 1186, 1828
BseMII	CTCAG	4 cut(s)	384, 603, 1410, 1599
BseRI	GAGGAG	5 cut(s)	51, 689, 1091, 1207, 1349
BseXI	GCAGC	4 cut(s)	293, 1389, 1719, 1831
BshFI	GGCC	6 cut(s)	314, 331, 495, 1003, 1259, 1327
BshNI	GGYRCC	1 cut(s)	435
BsiHKAI	GWGCWC	1 cut(s)	117
BsiHKCI	CYCGRG	2 cut(s)	270, 748
BsiSI	CCGG	7 cut(s)	263, 285, 332, 723, 749, 956, 1943
BslFI	GGGAC	4 cut(s)	725, 902, 937, 1081
BslI	CCNNNNNNNGG	6 cut(s)	748, 749, 755, 926, 1186, 1828
BsmAI	GTCTC	4 cut(s)	474, 804, 1571, 1871
BsmBI	CGTCTC	1 cut(s)	804
BsmFI	GGGAC	4 cut(s)	725, 902, 937, 1081
BsnI	GGCC	6 cut(s)	314, 331, 495, 1003, 1259, 1327
Bso31I	GGTCTC	2 cut(s)	1571, 1871
BsoBI	CYCGRG	2 cut(s)	270, 748
Bsp1286I	GDGCHC	1 cut(s)	117
Bsp13I	TCCGGA	1 cut(s)	1942
Bsp143I	GATC	3 cut(s)	31, 516, 1657
Bsp1720I	GCTNAGC	1 cut(s)	589
Bsp19I	CCATGG	2 cut(s)	341, 543
BspACI	CCGC	5 cut(s)	74, 743, 786, 1406, 1930
BspANI	GGCC	6 cut(s)	314, 331, 495, 1003, 1259, 1327
BspCNI	CTCAG	4 cut(s)	385, 602, 1409, 1598
BspEI	TCCGGA	1 cut(s)	1942
BspLI	GGNNCC	7 cut(s)	33, 267, 437, 494, 1069, 1098, 1549
BspPI	GGATC	3 cut(s)	26, 39, 511
BspT107I	GGYRCC	1 cut(s)	435
BspTNI	GGTCTC	2 cut(s)	1571, 1871
BsrBI	CCGCTC	1 cut(s)	76
BsrFI	RCCGGY	1 cut(s)	331
BssAI	RCCGGY	1 cut(s)	331
BssMI	GATC	3 cut(s)	31, 516, 1657
BssT1I	CCWWGG	7 cut(s)	341, 507, 543, 855, 870, 1277, 1827
Bst2UI	CCWGG	2 cut(s)	921, 1626
Bst4CI	ACNGT	4 cut(s)	250, 1229, 1285, 1431
BstAPI	GCANNNNNTGC	1 cut(s)	677
BstBAI	YACGTR	1 cut(s)	1612
BstC8I	GCNNGC	4 cut(s)	333, 356, 1393, 1963
BstDEI	CTNAG	5 cut(s)	393, 589, 1396, 1585, 2030
BstDSI	CCRYGG	2 cut(s)	341, 543
BstF5I	GGATG	3 cut(s)	411, 1259, 1939
BstH2I	RGCGCY	1 cut(s)	1521
BstHHI	GCGC	4 cut(s)	261, 816, 1520, 1655
BstKTI	GATC	3 cut(s)	34, 519, 1660
BstMAI	GTCTC	4 cut(s)	474, 804, 1571, 1871
BstMBI	GATC	3 cut(s)	31, 516, 1657
BstMWI	GCNNNNNNNGC	5 cut(s)	364, 548, 677, 1515, 1962
BstNI	CCWGG	2 cut(s)	921, 1626
BstNSI	RCATGY	2 cut(s)	358, 1019
BstPAI	GACNNNNGTC	1 cut(s)	1598
BstSCI	CCNGG	5 cut(s)	261, 747, 748, 919, 1624
BstSNI	TACGTA	1 cut(s)	1612
BstV1I	GCAGC	4 cut(s)	293, 1389, 1719, 1831
BstV2I	GAAGAC	4 cut(s)	1088, 1307, 1550, 1662
BstX2I	RGATCY	1 cut(s)	31
BstYI	RGATCY	1 cut(s)	31
BsuI	GTATCC	2 cut(s)	752, 979
BsuRI	GGCC	6 cut(s)	314, 331, 495, 1003, 1259, 1327
BtgI	CCRYGG	2 cut(s)	341, 543
BtrI	CACGTC	1 cut(s)	798
BtsCI	GGATG	3 cut(s)	411, 1259, 1939
BtsI	GCAGTG	1 cut(s)	507
Cac8I	GCNNGC	4 cut(s)	333, 356, 1393, 1963
CfoI	GCGC	4 cut(s)	261, 816, 1520, 1655
Cfr10I	RCCGGY	1 cut(s)	331
Cfr13I	GGNCC	9 cut(s)	329, 413, 493, 652, 1001, 1068, 1257, 1547, 1622
Cfr9I	CCCGGG	1 cut(s)	748
CsiI	ACCWGGT	1 cut(s)	1624
Csp6I	GTAC	6 cut(s)	49, 436, 562, 718, 793, 1936
CspCI	CAANNNNNGTGG	2 cut(s)	1266, 1301
CviAII	CATG	9 cut(s)	194, 342, 355, 363, 497, 544, 622, 911, 1016
CviQI	GTAC	6 cut(s)	49, 436, 562, 718, 793, 1936
DdeI	CTNAG	5 cut(s)	393, 589, 1396, 1585, 2030
DpnI	GATC	3 cut(s)	33, 518, 1659
DpnII	GATC	3 cut(s)	31, 516, 1657
DrdI	GACNNNNNNGTC	1 cut(s)	1554
DseDI	GACNNNNNNGTC	1 cut(s)	1554
EaeI	YGGCCR	2 cut(s)	312, 1325
EciI	GGCGGA	1 cut(s)	1919
Ecl136II	GAGCTC	1 cut(s)	115
Eco105I	TACGTA	1 cut(s)	1612
Eco130I	CCWWGG	7 cut(s)	341, 507, 543, 855, 870, 1277, 1827
Eco24I	GRGCYC	1 cut(s)	117
Eco31I	GGTCTC	2 cut(s)	1571, 1871
Eco47I	GGWCC	5 cut(s)	413, 652, 1068, 1547, 1622
Eco47III	AGCGCT	1 cut(s)	1519
Eco53kI	GAGCTC	1 cut(s)	115
Eco88I	CYCGRG	2 cut(s)	270, 748
EcoICRI	GAGCTC	1 cut(s)	115
EcoO109I	RGGNCCY	3 cut(s)	1068, 1547, 1622
EcoRI	GAATTC	1 cut(s)	1272
EcoRII	CCWGG	2 cut(s)	919, 1624
EcoT14I	CCWWGG	7 cut(s)	341, 507, 543, 855, 870, 1277, 1827
EcoT38I	GRGCYC	1 cut(s)	117
ErhI	CCWWGG	7 cut(s)	341, 507, 543, 855, 870, 1277, 1827
Esp3I	CGTCTC	1 cut(s)	804
FaeI	CATG	9 cut(s)	197, 345, 358, 366, 500, 547, 625, 914, 1019
FalI	AAGNNNNNCTT	2 cut(s)	1017, 1049
FaqI	GGGAC	4 cut(s)	725, 902, 937, 1081
FatI	CATG	9 cut(s)	193, 341, 354, 362, 496, 543, 621, 910, 1015
FauI	CCCGC	1 cut(s)	750
FauNDI	CATATG	1 cut(s)	707
FblI	GTMKAC	1 cut(s)	1602
Fnu4HI	GCNGC	5 cut(s)	282, 1403, 1406, 1708, 1845
FokI	GGATG	3 cut(s)	418, 1266, 1926
FriOI	GRGCYC	1 cut(s)	117
Fsp4HI	GCNGC	5 cut(s)	282, 1403, 1406, 1708, 1845
FspBI	CTAG	7 cut(s)	12, 119, 539, 737, 1029, 1812, 1848
GlaI	GCGC	4 cut(s)	260, 815, 1519, 1654
GluI	GCNGC	5 cut(s)	282, 1403, 1406, 1708, 1845
GsuI	CTGGAG	1 cut(s)	1542
HaeII	RGCGCY	1 cut(s)	1521
HaeIII	GGCC	6 cut(s)	314, 331, 495, 1003, 1259, 1327
HapII	CCGG	7 cut(s)	263, 285, 332, 723, 749, 956, 1943
HhaI	GCGC	4 cut(s)	261, 816, 1520, 1655
Hin1II	CATG	9 cut(s)	197, 345, 358, 366, 500, 547, 625, 914, 1019
Hin6I	GCGC	4 cut(s)	259, 814, 1518, 1653
HinP1I	GCGC	4 cut(s)	259, 814, 1518, 1653
HincII	GTYRAC	1 cut(s)	1759
HindII	GTYRAC	1 cut(s)	1759
HindIII	AAGCTT	4 cut(s)	81, 833, 1777, 1963
HpaII	CCGG	7 cut(s)	263, 285, 332, 723, 749, 956, 1943
HphI	GGTGA	4 cut(s)	521, 694, 1006, 1033
Hpy166II	GTNNAC	6 cut(s)	51, 325, 795, 1603, 1759, 1819
Hpy188I	TCNGA	8 cut(s)	31, 88, 95, 187, 295, 758, 1225, 1588
Hpy188III	TCNNGA	6 cut(s)	35, 67, 580, 1034, 1100, 1943
Hpy8I	GTNNAC	6 cut(s)	51, 325, 795, 1603, 1759, 1819
HpyAV	CCTTC	1 cut(s)	1243
HpyCH4III	ACNGT	4 cut(s)	250, 1229, 1285, 1431
HpyCH4IV	ACGT	2 cut(s)	797, 1611
HpyCH4V	TGCA	8 cut(s)	197, 470, 500, 671, 1246, 1509, 1571, 1922
HpyF10VI	GCNNNNNNNGC	5 cut(s)	364, 548, 677, 1515, 1962
HpyF3I	CTNAG	5 cut(s)	393, 589, 1396, 1585, 2030
HpySE526I	ACGT	2 cut(s)	797, 1611
Hsp92II	CATG	9 cut(s)	197, 345, 358, 366, 500, 547, 625, 914, 1019
HspAI	GCGC	4 cut(s)	259, 814, 1518, 1653
Kpn2I	TCCGGA	1 cut(s)	1942
KpnI	GGTACC	1 cut(s)	439
KroI	GCCGGC	1 cut(s)	331
KroNI	GCCGGC	1 cut(s)	333
Kzo9I	GATC	3 cut(s)	31, 516, 1657
LmnI	GCTCC	3 cut(s)	112, 120, 1523
Lsp1109I	GCAGC	4 cut(s)	293, 1389, 1719, 1831
LweI	GCATC	4 cut(s)	28, 132, 307, 762
MabI	ACCWGGT	1 cut(s)	1624
MaeI	CTAG	7 cut(s)	12, 119, 539, 737, 1029, 1812, 1848
MaeII	ACGT	2 cut(s)	797, 1611
MaeIII	GTNAC	6 cut(s)	773, 971, 1699, 1885, 1975, 1996
MalI	GATC	3 cut(s)	33, 518, 1659
MbiI	CCGCTC	1 cut(s)	76
MboI	GATC	3 cut(s)	31, 516, 1657
MboII	GAAGA	8 cut(s)	14, 409, 1093, 1312, 1550, 1639, 1662, 1806
MfeI	CAATTG	1 cut(s)	893
MflI	RGATCY	1 cut(s)	31
MhlI	GDGCHC	1 cut(s)	117
MlsI	TGGCCA	1 cut(s)	1327
MluNI	TGGCCA	1 cut(s)	1327
MlyI	GAGTC	4 cut(s)	585, 656, 1229, 1588
MmeI	TCCRAC	3 cut(s)	1190, 1413, 1437
Mox20I	TGGCCA	1 cut(s)	1327
MroI	TCCGGA	1 cut(s)	1942
MroNI	GCCGGC	1 cut(s)	331
MscI	TGGCCA	1 cut(s)	1327
MseI	TTAA	4 cut(s)	108, 837, 1689, 1967
MslI	CAYNNNNRTG	2 cut(s)	340, 1536
Msp20I	TGGCCA	1 cut(s)	1327
MspI	CCGG	7 cut(s)	263, 285, 332, 723, 749, 956, 1943
MspR9I	CCNGG	5 cut(s)	263, 749, 750, 921, 1626
MunI	CAATTG	1 cut(s)	893
MvaI	CCWGG	2 cut(s)	921, 1626
MwoI	GCNNNNNNNGC	5 cut(s)	364, 548, 677, 1515, 1962
NaeI	GCCGGC	1 cut(s)	333
NciI	CCSGG	3 cut(s)	263, 749, 750
NcoI	CCATGG	2 cut(s)	341, 543
NdeI	CATATG	1 cut(s)	707
NdeII	GATC	3 cut(s)	31, 516, 1657
NgoMIV	GCCGGC	1 cut(s)	331
NlaIII	CATG	9 cut(s)	197, 345, 358, 366, 500, 547, 625, 914, 1019
NlaIV	GGNNCC	7 cut(s)	33, 267, 437, 494, 1069, 1098, 1549
NmeAIII	GCCGAG	1 cut(s)	1471
NmuCI	GTSAC	3 cut(s)	773, 971, 1996
NspI	RCATGY	2 cut(s)	358, 1019
PaeI	GCATGC	1 cut(s)	358
PasI	CCCWGGG	1 cut(s)	920
PdiI	GCCGGC	1 cut(s)	333
PfeI	GAWTC	7 cut(s)	70, 188, 304, 457, 733, 1487, 1750
PkrI	GCNGC	5 cut(s)	283, 1404, 1407, 1709, 1846
PleI	GAGTC	4 cut(s)	584, 655, 1228, 1588
PpsI	GAGTC	4 cut(s)	584, 655, 1228, 1588
Ppu21I	YACGTR	1 cut(s)	1612
PpuMI	RGGWCCY	3 cut(s)	1068, 1547, 1622
PshAI	GACNNNNGTC	1 cut(s)	1598
Psp124BI	GAGCTC	1 cut(s)	117
Psp5II	RGGWCCY	3 cut(s)	1068, 1547, 1622
Psp6I	CCWGG	2 cut(s)	919, 1624
PspGI	CCWGG	2 cut(s)	919, 1624
PspN4I	GGNNCC	7 cut(s)	33, 267, 437, 494, 1069, 1098, 1549
PspPI	GGNCC	9 cut(s)	329, 413, 493, 652, 1001, 1068, 1257, 1547, 1622
PspPPI	RGGWCCY	3 cut(s)	1068, 1547, 1622
PsuI	RGATCY	1 cut(s)	31
RsaI	GTAC	6 cut(s)	50, 437, 563, 719, 794, 1937
RsaNI	GTAC	6 cut(s)	49, 436, 562, 718, 793, 1936
RseI	CAYNNNNRTG	2 cut(s)	340, 1536
SacI	GAGCTC	1 cut(s)	117
SaqAI	TTAA	4 cut(s)	108, 837, 1689, 1967
SatI	GCNGC	5 cut(s)	282, 1403, 1406, 1708, 1845
Sau3AI	GATC	3 cut(s)	31, 516, 1657
Sau96I	GGNCC	9 cut(s)	329, 413, 493, 652, 1001, 1068, 1257, 1547, 1622
ScaI	AGTACT	1 cut(s)	719
SchI	GAGTC	4 cut(s)	585, 656, 1229, 1588
ScrFI	CCNGG	5 cut(s)	263, 749, 750, 921, 1626
SduI	GDGCHC	1 cut(s)	117
SexAI	ACCWGGT	1 cut(s)	1624
SfaNI	GCATC	4 cut(s)	28, 132, 307, 762
SinI	GGWCC	5 cut(s)	413, 652, 1068, 1547, 1622
SmaI	CCCGGG	1 cut(s)	750
SmiMI	CAYNNNNRTG	2 cut(s)	340, 1536
SmlI	CTYRAG	2 cut(s)	801, 1785
SmoI	CTYRAG	2 cut(s)	801, 1785
SnaBI	TACGTA	1 cut(s)	1612
SphI	GCATGC	1 cut(s)	358
SsiI	CCGC	5 cut(s)	74, 743, 786, 1406, 1930
SspMI	CTAG	7 cut(s)	12, 119, 539, 737, 1029, 1812, 1848
SstI	GAGCTC	1 cut(s)	117
StyD4I	CCNGG	5 cut(s)	261, 747, 748, 919, 1624
StyI	CCWWGG	7 cut(s)	341, 507, 543, 855, 870, 1277, 1827
TaaI	ACNGT	4 cut(s)	250, 1229, 1285, 1431
TaiI	ACGT	2 cut(s)	800, 1614
TaqI	TCGA	7 cut(s)	78, 302, 962, 1371, 1452, 1766, 1773
TaqII	GACCGA	1 cut(s)	640
TatI	WGTACW	1 cut(s)	717
TauI	GCSGC	1 cut(s)	1408
TfiI	GAWTC	7 cut(s)	70, 188, 304, 457, 733, 1487, 1750
Tru1I	TTAA	4 cut(s)	108, 837, 1689, 1967
Tru9I	TTAA	4 cut(s)	108, 837, 1689, 1967
TseFI	GTSAC	3 cut(s)	773, 971, 1996
TseI	GCWGC	4 cut(s)	281, 1402, 1707, 1844
Tsp45I	GTSAC	3 cut(s)	773, 971, 1996
TspDTI	ATGAA	6 cut(s)	422, 899, 1050, 1746, 1928, 1981
TspGWI	ACGGA	2 cut(s)	761, 1379
TspMI	CCCGGG	1 cut(s)	748
VpaK11BI	GGWCC	5 cut(s)	413, 652, 1068, 1547, 1622
XapI	RAATTY	2 cut(s)	1272, 1988
XceI	RCATGY	2 cut(s)	358, 1019
XmaI	CCCGGG	1 cut(s)	748
XmiI	GTMKAC	1 cut(s)	1602
XspI	CTAG	7 cut(s)	12, 119, 539, 737, 1029, 1812, 1848
ZrmI	AGTACT	1 cut(s)	719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.