Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0010222

Physical Location & Seq

Genomic Coordinates Forward (+)

48946594 .. 48951281

Gene Structure 4688 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00254670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2079 bp

ATGGCAGACACAAGACCTTTGCTTCCGTCCGACCCCGATGCCCTTCCCACCGCCTACACTCCTCTCCCAGGGGGATCCTCCTATCCGGAGATCCGGCGGATACACGTGAAGGAGGTTCTGGCGATGTTCGCCGGAGTGGTGATGGTTTCTTTGTTGGTGGTCATTATAGGGAATACAGGTCACGATGGAAATGTGTACCATAGGAACAATAGGAGGGATAAGCCAATGTTATTGGCTGCTTCACCGACCAAGGAAACTACCACCAAGGCAGAGAAGTTGCAGGCGGTGACACGTGGCATGGCGGCCGGAGTGTCGGAGAAATCGAGCCGGTTGTACAGCAGCGGCGTTGGTGCGGGCACGCCGGTGTATCCGTGGAACAATAGCATGTTGTCGTGGCAGAGGACTGCTTTCCATTTTCAGCCGGAGAAGAATTGGATGAATGATCCCAACGGTCCATTATTCTACAAGGGTTGGTACCATTTTTTCTACCAGTACAATCCCAAAGGTGCAGTATGGGGAAACATAGTATGGGGTCATGCAGTATCCAGAGACTTGATTCATTGGCTTCACCTTCCTCTAGCAATGGTAGCTGATCAATGGTATGACATCAATGGAGTCTGGACTGGCTCGGCTACAATTCTCCCAAATGACCAAATTGTCATGCTCTACACTGGTTCAACCAATGAGTCAGTCCAGGTCCAATGTCTTGCATACCCTGCTGACCACAAAGACCCTCTCCTCACCAAGTGGGTCAAGTATTCCGGAAACCCGGTTCTAGTCCCCCCTCCCGGGATCGGAGTCAAGGACTTCCGGGACCCGACCACTGCTTGGTACACTACTGAAGGGAAATGGAGGATTACAATTGGGTCCAAGGTTAACAAAACTGGTATATCTTTGGTTTATGACACCAAGGACTTTATCAAGTATGAGCAACTAGATGGTGTGCTTCATGCTGTGCCTGGGACTGGAATGTGGGAATGTATCGATTTTTATCCGGTGTCGAAAATTAGTGACAAGGGGTTGGACACTTCTCAGAATGGGGCTGATGTGAAGCATGTGATGAAGACTAGTCTTGATGATGATAGGAATGATTACTATGCATTGGGGTCTTATAATGAGAAGACTGGAAAATGGGTTCCTGATAATCAAAAGATTGATGTTGGGATTGGGATTAGGTATGATTATGGGAAATTCTATGCTTCCAAGACCTTCTATGATCAGAACAAGCAGAGAAGGGTGTTGTGGGGTTGGATTGGAGAGTCTGATAGTGAGAATGCTGATGTCAAGAAGGGTTGGGCATCTCTTCAGGGAATTCCAAGAACAGTGTTGTTTGATCAAAAGACTGGTAGCAATCTACTTCAATGGCCAGTGGAGGAGATTGAGAAATTGAGATTGAACAAGAAGAACTTCGACAAGGTACAAGTCAAGGCAGGGTCAGTGGTGCCACTTGACGTTGGCACAGCTACACAGTTGGACATTGTCGCCGAGTTTGAGTTGGATCAGAAGGTTGTAGAGAGCGCAGCTGAATCCAACGAGGAGTTCAGTTGCCAAACTAGTGGTGGAGCTGCCAAACGCGGCGCATTGGGACCCTTTGGTCTTCTTGTTCTTGCAGATGACAGCCTCTCTGAGCGCACTCCTGTCTACTTTTATGTTGTCAAAGGATCTGGTGGCACTGTCAATACCTACTTCTGTGCTGATCAAACAAGGTCTTCTGTGGCAAATGATGTTGTTAAACAAGTTAGTGGTAGCTATGTTCCAGTACTGAAAGGTGAAAAGCTATCTCTGAGAATACTGGTTGATCATTCAATAATTGAAAGCTTTGCTCAAGGTGGACGAACAACGATAACAACGCGCGTTTACCCAACTCAGGCAATTTATGGAGCTGCAAGGCTCTTTTTGTTCAACAATGCTACTGACACCAGTGTTACTGCCTCACTCCAGATATGGCAGATGAACTCTGCATTCATACGCCCATTCTCACCTGATGCAGCGAGTCATGCAAGCTTCACCCCTGTTACAGTATTTATCAAATTCATCGTCCCATTTGGGATTTTTTTAACTTTATATTTTGTAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0004553 GO:0004564 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0007275 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009739 GO:0009987 GO:0010033 GO:0016787 GO:0016798 GO:0022622 GO:0030312 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048731 GO:0048856 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071944 GO:0080022 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00052 ko00500 ko01100 map00052 map00500 map01100

Pfam Domains

Protein Families

DUF3357 Glyco_hydro_32C Glyco_hydro_32N

Protein Analysis

692

Amino Acids

76.91

Weight (kDa)

8.04

Isoelectric Point (pI)

31.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
INV_N	PF11837	19 - 130	1.6e-16	Beta-fructofuranosidase, N-terminal domain
Glyco_hydro_32N	PF00251	138 - 456	8.5e-105	Glycosyl hydrolases family 32 N-terminal domain
Glyco_hydro_32C	PF08244	459 - 650	1.4e-25	Glycosyl hydrolases family 32 C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12240 AT1G62660 AT1G62660 AT1G62660 AT1G62660
fragaria_vesca	FvH4_5g00290 FvH4_5g00290 FvH4_5g00290
malus_domestica	MD06G1066600.v1.1
prunus_persica	Prupe.5G075600_v2.0.a1 Prupe.5G075600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0202311
rosa_laevigata	RLG00000003612
rosa_multiflora	Rmu_sc0010249.1_g000001
rosa_roxburghii	Rroxscaffold_3G00254670
rosa_rugosa	Rorug07G0069800
rosa_samantha	Rh7AG196900 Rh7BG198200 Rh7CG208100 Rh7DG203600
rosa_wichuraiana	Rw7G017260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1113
AasI	GACNNNNNNGTC	2 cut(s)	656, 1593
Acc65I	GGTACC	1 cut(s)	474
AccB1I	GGYRCC	2 cut(s)	474, 1441
AccB7I	CCANNNNNTGG	1 cut(s)	828
AccI	GTMKAC	1 cut(s)	1641
AccII	CGCG	3 cut(s)	1575, 1852, 1854
AccIII	TCCGGA	2 cut(s)	85, 761
AciI	CCGC	7 cut(s)	51, 97, 284, 302, 342, 353, 1575
AclWI	GGATC	7 cut(s)	69, 82, 85, 437, 800, 1506, 1669
AcoI	YGGCCR	2 cut(s)	303, 1364
AcsI	RAATTY	3 cut(s)	1190, 1311, 2030
AcuI	CTGAAG	2 cut(s)	861, 1289
AcvI	CACGTG	2 cut(s)	106, 293
AdeI	CACNNNGTG	1 cut(s)	747
AfaI	GTAC	7 cut(s)	197, 335, 476, 494, 833, 1419, 1761
AfiI	CCNNNNNNNGG	7 cut(s)	68, 788, 789, 794, 828, 965, 1867
AflIII	ACRYGT	2 cut(s)	103, 290
AgsI	TTSAA	6 cut(s)	678, 1361, 1396, 1806, 1814, 1903
AhlI	ACTAGT	2 cut(s)	1067, 1553
AjnI	CCWGG	3 cut(s)	67, 693, 958
AjuI	GAANNNNNNNTTGG	2 cut(s)	1523, 1555
AleI	CACNNNNGTG	1 cut(s)	362
AloI	GAACNNNNNNTCC	4 cut(s)	756, 788, 1119, 1151
AluBI	AGCT	9 cut(s)	590, 1463, 1523, 1565, 1749, 1777, 1818, 1883, 2004
AluI	AGCT	9 cut(s)	590, 1463, 1523, 1565, 1749, 1777, 1818, 1883, 2004
Alw26I	GTCTC	1 cut(s)	543
AlwI	GGATC	7 cut(s)	69, 82, 85, 437, 800, 1506, 1669
Ama87I	CYCGRG	1 cut(s)	788
Aor13HI	TCCGGA	2 cut(s)	85, 761
AoxI	GGCC	2 cut(s)	303, 1364
ApeKI	GCWGC	6 cut(s)	236, 339, 1520, 1565, 1883, 1988
ApoI	RAATTY	3 cut(s)	1190, 1311, 2030
ArsI	GACNNNNNNTTYG	2 cut(s)	2022, 2054
Asp700I	GAANNNNTTC	1 cut(s)	1406
Asp718I	GGTACC	1 cut(s)	474
AspLEI	GCGC	4 cut(s)	1520, 1580, 1632, 1854
AspS9I	GGNCC	5 cut(s)	452, 697, 814, 867, 1586
AsuC2I	CCSGG	4 cut(s)	770, 789, 790, 812
AsuHPI	GGTGA	8 cut(s)	151, 234, 298, 560, 733, 1781, 1971, 1999
AvaI	CYCGRG	1 cut(s)	788
AvaII	GGWCC	5 cut(s)	452, 697, 814, 867, 1586
BaeGI	GKGCMC	1 cut(s)	359
BalI	TGGCCA	1 cut(s)	1366
BamHI	GGATCC	1 cut(s)	74
BanI	GGYRCC	2 cut(s)	474, 1441
BbrPI	CACGTG	2 cut(s)	106, 293
BbsI	GAAGAC	4 cut(s)	1070, 1127, 1589, 1701
BbvI	GCAGC	6 cut(s)	223, 351, 1532, 1552, 1870, 2000
BccI	CCATC	3 cut(s)	136, 179, 932
BcgI	CGANNNNNNTGC	2 cut(s)	20, 54
BciT130I	CCWGG	3 cut(s)	69, 695, 960
BciVI	GTATCC	3 cut(s)	93, 378, 553
BclI	TGATCA	5 cut(s)	592, 1216, 1333, 1696, 1798
BcnI	CCSGG	4 cut(s)	770, 789, 790, 812
BcoDI	GTCTC	1 cut(s)	543
BcuI	ACTAGT	2 cut(s)	1067, 1553
BfaI	CTAG	5 cut(s)	578, 776, 935, 1068, 1554
BfuI	GTATCC	3 cut(s)	93, 378, 553
BisI	GCNGC	9 cut(s)	237, 303, 340, 343, 1521, 1566, 1576, 1884, 1989
BlsI	GCNGC	9 cut(s)	238, 304, 341, 344, 1522, 1567, 1577, 1885, 1990
BmcAI	AGTACT	1 cut(s)	1761
Bme1390I	CCNGG	7 cut(s)	69, 695, 770, 789, 790, 812, 960
Bme18I	GGWCC	5 cut(s)	452, 697, 814, 867, 1586
BmeT110I	CYCGRG	1 cut(s)	788
BmgT120I	GGNCC	5 cut(s)	452, 697, 814, 867, 1586
BmiI	GGNNCC	9 cut(s)	76, 476, 815, 816, 868, 1137, 1443, 1587, 1588
BmrFI	CCNGG	7 cut(s)	69, 695, 770, 789, 790, 812, 960
BmsI	GCATC	3 cut(s)	28, 1307, 1975
BpiI	GAAGAC	4 cut(s)	1070, 1127, 1589, 1701
BpmI	CTGGAG	1 cut(s)	1922
BpuEI	CTTGAG	1 cut(s)	1809
BpuMI	CCSGG	4 cut(s)	770, 789, 790, 812
Bsa29I	ATCGAT	1 cut(s)	984
BsaAI	YACGTR	2 cut(s)	106, 293
BsaBI	GATNNNNATC	1 cut(s)	990
BsaJI	CCNNGG	9 cut(s)	67, 68, 249, 264, 371, 788, 870, 909, 959
BsaWI	WCCGGW	3 cut(s)	85, 761, 994
Bsc4I	CCNNNNNNNGG	7 cut(s)	68, 788, 789, 794, 828, 965, 1867
Bse118I	RCCGGY	2 cut(s)	327, 361
Bse3DI	GCAATG	1 cut(s)	588
Bse8I	GATNNNNATC	1 cut(s)	990
BseAI	TCCGGA	2 cut(s)	85, 761
BseBI	CCWGG	3 cut(s)	69, 695, 960
BseCI	ATCGAT	1 cut(s)	984
BseDI	CCNNGG	9 cut(s)	67, 68, 249, 264, 371, 788, 870, 909, 959
BseGI	GGATG	1 cut(s)	441
BseJI	GATNNNNATC	1 cut(s)	990
BseLI	CCNNNNNNNGG	7 cut(s)	68, 788, 789, 794, 828, 965, 1867
BseMI	GCAATG	1 cut(s)	588
BseMII	CTCAG	4 cut(s)	1046, 1617, 1775, 1880
BseRI	GAGGAG	4 cut(s)	51, 728, 1388, 1550
BseSI	GKGCMC	1 cut(s)	359
BseX3I	CGGCCG	1 cut(s)	303
BseXI	GCAGC	6 cut(s)	223, 351, 1532, 1552, 1870, 2000
BsgI	GTGCAG	1 cut(s)	528
Bsh1236I	CGCG	3 cut(s)	1575, 1852, 1854
Bsh1285I	CGRYCG	1 cut(s)	306
BshFI	GGCC	2 cut(s)	305, 1366
BshNI	GGYRCC	2 cut(s)	474, 1441
BshVI	ATCGAT	1 cut(s)	984
BsiEI	CGRYCG	1 cut(s)	306
BsiHKCI	CYCGRG	1 cut(s)	788
BslFI	GGGAC	5 cut(s)	764, 827, 976, 1599, 2024
BslI	CCNNNNNNNGG	7 cut(s)	68, 788, 789, 794, 828, 965, 1867
BsmAI	GTCTC	1 cut(s)	543
BsmFI	GGGAC	5 cut(s)	764, 827, 976, 1599, 2024
BsmI	GAATGC	2 cut(s)	1279, 1961
BsnI	GGCC	2 cut(s)	305, 1366
BsoBI	CYCGRG	1 cut(s)	788
Bsp1286I	GDGCHC	1 cut(s)	359
Bsp13I	TCCGGA	2 cut(s)	85, 761
Bsp1407I	TGTACA	1 cut(s)	333
BspACI	CCGC	7 cut(s)	51, 97, 284, 302, 342, 353, 1575
BspANI	GGCC	2 cut(s)	305, 1366
BspCNI	CTCAG	4 cut(s)	1045, 1618, 1776, 1879
BspDI	ATCGAT	1 cut(s)	984
BspEI	TCCGGA	2 cut(s)	85, 761
BspFNI	CGCG	3 cut(s)	1575, 1852, 1854
BspLI	GGNNCC	9 cut(s)	76, 476, 815, 816, 868, 1137, 1443, 1587, 1588
BspPI	GGATC	7 cut(s)	69, 82, 85, 437, 800, 1506, 1669
BspT107I	GGYRCC	2 cut(s)	474, 1441
BsrDI	GCAATG	1 cut(s)	588
BsrFI	RCCGGY	2 cut(s)	327, 361
BsrGI	TGTACA	1 cut(s)	333
BssAI	RCCGGY	2 cut(s)	327, 361
BssECI	CCNNGG	9 cut(s)	67, 68, 249, 264, 371, 788, 870, 909, 959
BssT1I	CCWWGG	4 cut(s)	249, 264, 870, 909
Bst2UI	CCWGG	3 cut(s)	69, 695, 960
Bst4CI	ACNGT	5 cut(s)	452, 1324, 1470, 1675, 2020
Bst6I	CTCTTC	1 cut(s)	1308
BstAPI	GCANNNNNTGC	1 cut(s)	716
BstAUI	TGTACA	1 cut(s)	333
BstBAI	YACGTR	2 cut(s)	106, 293
BstC8I	GCNNGC	4 cut(s)	282, 355, 359, 2002
BstDEI	CTNAG	4 cut(s)	1032, 1626, 1784, 1866
BstDSI	CCRYGG	1 cut(s)	371
BstENI	CCTNNNNNAGG	1 cut(s)	66
BstF5I	GGATG	1 cut(s)	441
BstFNI	CGCG	3 cut(s)	1575, 1852, 1854
BstHHI	GCGC	4 cut(s)	1520, 1580, 1632, 1854
BstMAI	GTCTC	1 cut(s)	543
BstMCI	CGRYCG	1 cut(s)	306
BstMWI	GCNNNNNNNGC	4 cut(s)	128, 587, 716, 1997
BstNI	CCWGG	3 cut(s)	69, 695, 960
BstNSI	RCATGY	2 cut(s)	388, 1058
BstSCI	CCNGG	7 cut(s)	67, 693, 768, 787, 788, 810, 958
BstSLI	GKGCMC	1 cut(s)	359
BstUI	CGCG	3 cut(s)	1575, 1852, 1854
BstV1I	GCAGC	6 cut(s)	223, 351, 1532, 1552, 1870, 2000
BstV2I	GAAGAC	4 cut(s)	1070, 1127, 1589, 1701
BstX2I	RGATCY	3 cut(s)	74, 90, 1661
BstXI	CCANNNNNNTGG	1 cut(s)	1556
BstYI	RGATCY	3 cut(s)	74, 90, 1661
BstZI	CGGCCG	1 cut(s)	303
Bsu15I	ATCGAT	1 cut(s)	984
BsuI	GTATCC	3 cut(s)	93, 378, 553
BsuRI	GGCC	2 cut(s)	305, 1366
BsuTUI	ATCGAT	1 cut(s)	984
BtgI	CCRYGG	1 cut(s)	371
BtgZI	GCGATG	1 cut(s)	137
BtsCI	GGATG	1 cut(s)	441
BtsI	GCAGTG	1 cut(s)	822
BtsIMutI	CAGTG	7 cut(s)	669, 822, 1329, 1374, 1443, 1671, 1927
Cac8I	GCNNGC	4 cut(s)	282, 355, 359, 2002
CfoI	GCGC	4 cut(s)	1520, 1580, 1632, 1854
Cfr10I	RCCGGY	2 cut(s)	327, 361
Cfr13I	GGNCC	5 cut(s)	452, 697, 814, 867, 1586
Cfr9I	CCCGGG	1 cut(s)	788
ClaI	ATCGAT	1 cut(s)	984
Csp6I	GTAC	7 cut(s)	196, 334, 475, 493, 832, 1418, 1760
CviAII	CATG	7 cut(s)	298, 385, 536, 661, 950, 1055, 1997
CviQI	GTAC	7 cut(s)	196, 334, 475, 493, 832, 1418, 1760
DdeI	CTNAG	4 cut(s)	1032, 1626, 1784, 1866
DraIII	CACNNNGTG	1 cut(s)	747
DrdI	GACNNNNNNGTC	2 cut(s)	656, 1593
DseDI	GACNNNNNNGTC	2 cut(s)	656, 1593
EaeI	YGGCCR	2 cut(s)	303, 1364
EagI	CGGCCG	1 cut(s)	303
Eam1104I	CTCTTC	1 cut(s)	1308
EarI	CTCTTC	1 cut(s)	1308
EciI	GGCGGA	1 cut(s)	112
EclXI	CGGCCG	1 cut(s)	303
Eco130I	CCWWGG	4 cut(s)	249, 264, 870, 909
Eco47I	GGWCC	5 cut(s)	452, 697, 814, 867, 1586
Eco52I	CGGCCG	1 cut(s)	303
Eco57I	CTGAAG	2 cut(s)	861, 1289
Eco72I	CACGTG	2 cut(s)	106, 293
Eco88I	CYCGRG	1 cut(s)	788
EcoNI	CCTNNNNNAGG	1 cut(s)	66
EcoO109I	RGGNCCY	2 cut(s)	814, 1586
EcoRI	GAATTC	1 cut(s)	1311
EcoRII	CCWGG	3 cut(s)	67, 693, 958
EcoT14I	CCWWGG	4 cut(s)	249, 264, 870, 909
EcoT22I	ATGCAT	1 cut(s)	1102
ErhI	CCWWGG	4 cut(s)	249, 264, 870, 909
FaeI	CATG	7 cut(s)	301, 388, 539, 664, 953, 1058, 2000
FalI	AAGNNNNNCTT	2 cut(s)	1391, 1423
FaqI	GGGAC	5 cut(s)	764, 827, 976, 1599, 2024
FatI	CATG	7 cut(s)	297, 384, 535, 660, 949, 1054, 1996
FauI	CCCGC	1 cut(s)	346
FbaI	TGATCA	5 cut(s)	592, 1216, 1333, 1696, 1798
FblI	GTMKAC	1 cut(s)	1641
Fnu4HI	GCNGC	9 cut(s)	237, 303, 340, 343, 1521, 1566, 1576, 1884, 1989
FokI	GGATG	1 cut(s)	448
Fsp4HI	GCNGC	9 cut(s)	237, 303, 340, 343, 1521, 1566, 1576, 1884, 1989
FspBI	CTAG	5 cut(s)	578, 776, 935, 1068, 1554
GlaI	GCGC	4 cut(s)	1519, 1579, 1631, 1853
GluI	GCNGC	9 cut(s)	237, 303, 340, 343, 1521, 1566, 1576, 1884, 1989
GsuI	CTGGAG	1 cut(s)	1922
HaeIII	GGCC	2 cut(s)	305, 1366
HhaI	GCGC	4 cut(s)	1520, 1580, 1632, 1854
Hin1II	CATG	7 cut(s)	301, 388, 539, 664, 953, 1058, 2000
Hin6I	GCGC	4 cut(s)	1518, 1578, 1630, 1852
HinP1I	GCGC	4 cut(s)	1518, 1578, 1630, 1852
HincII	GTYRAC	1 cut(s)	877
HindII	GTYRAC	1 cut(s)	877
HindIII	AAGCTT	2 cut(s)	1816, 2002
HinfI	GANTC	7 cut(s)	556, 615, 686, 798, 1259, 1526, 1993
HpaI	GTTAAC	1 cut(s)	877
HphI	GGTGA	8 cut(s)	151, 234, 298, 560, 733, 1781, 1971, 1999
Hpy166II	GTNNAC	6 cut(s)	196, 834, 877, 1642, 1832, 1858
Hpy188I	TCNGA	9 cut(s)	31, 316, 797, 1035, 1221, 1264, 1503, 1627, 1785
Hpy188III	TCNNGA	9 cut(s)	86, 182, 546, 619, 762, 1073, 1139, 1285, 1939
Hpy8I	GTNNAC	6 cut(s)	196, 834, 877, 1642, 1832, 1858
HpyAV	CCTTC	8 cut(s)	53, 103, 581, 836, 1219, 1227, 1282, 1498
HpyCH4III	ACNGT	5 cut(s)	452, 1324, 1470, 1675, 2020
HpyCH4IV	ACGT	3 cut(s)	105, 292, 1452
HpyF10VI	GCNNNNNNNGC	4 cut(s)	128, 587, 716, 1997
HpyF3I	CTNAG	4 cut(s)	1032, 1626, 1784, 1866
HpySE526I	ACGT	3 cut(s)	105, 292, 1452
Hsp92II	CATG	7 cut(s)	301, 388, 539, 664, 953, 1058, 2000
HspAI	GCGC	4 cut(s)	1518, 1578, 1630, 1852
KflI	GGGWCCC	2 cut(s)	814, 1586
Kpn2I	TCCGGA	2 cut(s)	85, 761
KpnI	GGTACC	1 cut(s)	478
Ksp22I	TGATCA	5 cut(s)	592, 1216, 1333, 1696, 1798
KspAI	GTTAAC	1 cut(s)	877
LmnI	GCTCC	2 cut(s)	1562, 1880
Lsp1109I	GCAGC	6 cut(s)	223, 351, 1532, 1552, 1870, 2000
LweI	GCATC	3 cut(s)	28, 1307, 1975
MaeI	CTAG	5 cut(s)	578, 776, 935, 1068, 1554
MaeII	ACGT	3 cut(s)	105, 292, 1452
MaeIII	GTNAC	5 cut(s)	179, 286, 1010, 1924, 2014
MboII	GAAGA	7 cut(s)	439, 1075, 1132, 1295, 1414, 1589, 1701
MfeI	CAATTG	1 cut(s)	861
MflI	RGATCY	3 cut(s)	74, 90, 1661
MhlI	GDGCHC	1 cut(s)	359
MlsI	TGGCCA	1 cut(s)	1366
MluNI	TGGCCA	1 cut(s)	1366
MlyI	GAGTC	5 cut(s)	624, 695, 807, 1268, 2002
MmeI	TCCRAC	7 cut(s)	54, 294, 1002, 1229, 1452, 1476, 1554
Mox20I	TGGCCA	1 cut(s)	1366
Mph1103I	ATGCAT	1 cut(s)	1102
MroI	TCCGGA	2 cut(s)	85, 761
MroXI	GAANNNNTTC	1 cut(s)	1406
MscI	TGGCCA	1 cut(s)	1366
MseI	TTAA	3 cut(s)	876, 1731, 2057
MslI	CAYNNNNRTG	1 cut(s)	362
Msp20I	TGGCCA	1 cut(s)	1366
MspA1I	CMGCKG	2 cut(s)	342, 1523
MspR9I	CCNGG	7 cut(s)	69, 695, 770, 789, 790, 812, 960
MunI	CAATTG	1 cut(s)	861
Mva1269I	GAATGC	2 cut(s)	1279, 1961
MvaI	CCWGG	3 cut(s)	69, 695, 960
MvnI	CGCG	3 cut(s)	1575, 1852, 1854
MwoI	GCNNNNNNNGC	4 cut(s)	128, 587, 716, 1997
NciI	CCSGG	4 cut(s)	770, 789, 790, 812
NlaIII	CATG	7 cut(s)	301, 388, 539, 664, 953, 1058, 2000
NlaIV	GGNNCC	9 cut(s)	76, 476, 815, 816, 868, 1137, 1443, 1587, 1588
NmeAIII	GCCGAG	2 cut(s)	608, 1510
NmuCI	GTSAC	3 cut(s)	179, 286, 1010
NsiI	ATGCAT	1 cut(s)	1102
NspI	RCATGY	2 cut(s)	388, 1058
OliI	CACNNNNGTG	1 cut(s)	362
PasI	CCCWGGG	1 cut(s)	68
PcsI	WCGNNNNNNNCGW	1 cut(s)	320
PctI	GAATGC	2 cut(s)	1279, 1961
PdmI	GAANNNNTTC	1 cut(s)	1406
PfeI	GAWTC	2 cut(s)	556, 1526
PflFI	GACNNNGTC	1 cut(s)	1478
PflMI	CCANNNNNTGG	1 cut(s)	828
PfoI	TCCNGGA	1 cut(s)	810
PkrI	GCNGC	9 cut(s)	238, 304, 341, 344, 1522, 1567, 1577, 1885, 1990
PleI	GAGTC	5 cut(s)	623, 694, 806, 1267, 2001
PmaCI	CACGTG	2 cut(s)	106, 293
PmlI	CACGTG	2 cut(s)	106, 293
PpsI	GAGTC	5 cut(s)	623, 694, 806, 1267, 2001
Ppu21I	YACGTR	2 cut(s)	106, 293
PpuMI	RGGWCCY	2 cut(s)	814, 1586
PsiI	TTATAA	1 cut(s)	1113
Psp5II	RGGWCCY	2 cut(s)	814, 1586
Psp6I	CCWGG	3 cut(s)	67, 693, 958
PspCI	CACGTG	2 cut(s)	106, 293
PspGI	CCWGG	3 cut(s)	67, 693, 958
PspN4I	GGNNCC	9 cut(s)	76, 476, 815, 816, 868, 1137, 1443, 1587, 1588
PspPI	GGNCC	5 cut(s)	452, 697, 814, 867, 1586
PspPPI	RGGWCCY	2 cut(s)	814, 1586
PsuI	RGATCY	3 cut(s)	74, 90, 1661
PsyI	GACNNNGTC	1 cut(s)	1478
PvuII	CAGCTG	1 cut(s)	1523
RsaI	GTAC	7 cut(s)	197, 335, 476, 494, 833, 1419, 1761
RsaNI	GTAC	7 cut(s)	196, 334, 475, 493, 832, 1418, 1760
RseI	CAYNNNNRTG	1 cut(s)	362
SaqAI	TTAA	3 cut(s)	876, 1731, 2057
SatI	GCNGC	9 cut(s)	237, 303, 340, 343, 1521, 1566, 1576, 1884, 1989
Sau96I	GGNCC	5 cut(s)	452, 697, 814, 867, 1586
ScaI	AGTACT	1 cut(s)	1761
SchI	GAGTC	5 cut(s)	624, 695, 807, 1268, 2002
ScrFI	CCNGG	7 cut(s)	69, 695, 770, 789, 790, 812, 960
SduI	GDGCHC	1 cut(s)	359
SfaNI	GCATC	3 cut(s)	28, 1307, 1975
SgrAI	CRCCGGYG	1 cut(s)	361
SinI	GGWCC	5 cut(s)	452, 697, 814, 867, 1586
SmaI	CCCGGG	1 cut(s)	790
SmiMI	CAYNNNNRTG	1 cut(s)	362
SmlI	CTYRAG	1 cut(s)	1824
SmoI	CTYRAG	1 cut(s)	1824
SpeI	ACTAGT	2 cut(s)	1067, 1553
SsiI	CCGC	7 cut(s)	51, 97, 284, 302, 342, 353, 1575
SspMI	CTAG	5 cut(s)	578, 776, 935, 1068, 1554
StyD4I	CCNGG	7 cut(s)	67, 693, 768, 787, 788, 810, 958
StyI	CCWWGG	4 cut(s)	249, 264, 870, 909
TaaI	ACNGT	5 cut(s)	452, 1324, 1470, 1675, 2020
TaiI	ACGT	3 cut(s)	108, 295, 1455
TaqI	TCGA	4 cut(s)	323, 984, 1001, 1410
TatI	WGTACW	3 cut(s)	333, 492, 1759
TauI	GCSGC	3 cut(s)	305, 345, 1578
TfiI	GAWTC	2 cut(s)	556, 1526
Tru1I	TTAA	3 cut(s)	876, 1731, 2057
Tru9I	TTAA	3 cut(s)	876, 1731, 2057
TscAI	CASTG	7 cut(s)	676, 829, 1329, 1374, 1443, 1678, 1927
TseFI	GTSAC	3 cut(s)	179, 286, 1010
TseI	GCWGC	6 cut(s)	236, 339, 1520, 1565, 1883, 1988
Tsp45I	GTSAC	3 cut(s)	179, 286, 1010
TspDTI	ATGAA	7 cut(s)	452, 548, 938, 1076, 1954, 1967, 2023
TspGWI	ACGGA	2 cut(s)	15, 360
TspMI	CCCGGG	1 cut(s)	788
TspRI	CASTG	7 cut(s)	676, 829, 1329, 1374, 1443, 1678, 1927
Tth111I	GACNNNGTC	1 cut(s)	1478
Van91I	CCANNNNNTGG	1 cut(s)	828
VpaK11BI	GGWCC	5 cut(s)	452, 697, 814, 867, 1586
XagI	CCTNNNNNAGG	1 cut(s)	66
XapI	RAATTY	3 cut(s)	1190, 1311, 2030
XceI	RCATGY	2 cut(s)	388, 1058
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1556
XmaI	CCCGGG	1 cut(s)	788
XmiI	GTMKAC	1 cut(s)	1641
XmnI	GAANNNNTTC	1 cut(s)	1406
XspI	CTAG	5 cut(s)	578, 776, 935, 1068, 1554
ZrmI	AGTACT	1 cut(s)	1761
Zsp2I	ATGCAT	1 cut(s)	1102

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.