Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0010222

Physical Location & Seq

Genomic Coordinates Forward (+)

203947 .. 208493

Gene Structure 4547 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g00290.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2085 bp

ATGGCAGACAGAAGGCCTTTGCTTCCGTCCGACCCCGATGCCCTTCCCACAGCCTACGCCGGATCCTCCTCCTCTCCGGAGATCCGGCGGATACACGTGAAGGAGGTCCTGGCGATGTTCGCCGGGGTTGTGATGGTTTCTTTGTTGGTGGTCATTATAGGGAATACTGGTCATGATCAGGAGGCCGGGAATGCCAGCCACCACCGGAACTACATGCCTGATAAGCCAATGCCATTGACTTCGTCTTCGCCGACCAATGAGACTGCGACCATACCAGACAAGTTGCAGGCGGTGTCACGTGGCATGGCGGCCGGAGTGTCGGAGAAGTCGAGCCGGTTGTACAGCAGCGGCTTCGGGGCAGGCACGCCGGTGTATCCGTGGAACAATAGCATGTTGTCGTGGCAGAGGACTGCCTTCCATTTTCAGCCGGAGAAGAATTGGATGAATGATCCCAACGGTCCATTATTCTACAAGGGTTGGTACCATTTTTTCTACCAGTACAATCCCAAAGGTGCAGTTTGGGGAAACATAGTATGGGGTCATGCAGTATCCAGAGACTTGATCCATTGGCTTCACCTTCCTCTAGCAATGGTAGCTGATCAATGGTATGACATCAATGGAGTCTGGACTGGCTCTGCTACAATTCTCCCCAATGGCCAAATTGTCATGCTCTACACTGGTTCAACCAACGAGTCCGTCCAGGTCCAATGTCTTGCATACCCTGCTGACCACAATGACCCTCTCCTCACCAAGTGGATCAAGTACTCCGGCAACCCGGTTCTATTCCCCCCTCCCGGGATCGGAATCAAGGACTTCCGGGACCCGACCACCGCTTGGTACACCAAAGAAGGCAAATGGAGGATCACAATCGGGTCCAAGGTTAACAAAACTGGTATATCTTTAGTTTATGACACCAAGGACTTTATTAAGTATGAGCAGCTTGATGGTGTGCTTCATGCTGTGCCTGGTACTGGAATGTGGGAATGCATTGATTTTTATCCGGTGTCGAAAACTAGTGACAAGGGGCTGGACACTTCTGAGAATGGTGCTGATGTGAAGCATGTGATAAAGACTAGCCTTGATGATGATAGGAATGATTACTATGCATTGGGGAGTTATAACGAGCAGACTGGAAAATGGGTTCCTGATAATCCAAACATTGATGTTGGGATTGGGATTAGGTATGATTATGGGAAATTCTATGCTTCCAAGACCTTCTATGATCAGAACAAGCAGAGAAGGGTGTTGTGGGGATGGATTGGAGAGTCTGATAGTGAAAATGCTGATGTCCAGAAGGGTTGGGCATCTCTTCAGGGAATTCCAAGAACAGTGTTGTTTGATCAAAAGACTGGTAGCAATCTACTTCAATGGCCAGTGGAGGAGATTGAGAAATTGAGATTGAATATGAACAACTTCGACAGAGTACAAGTCAAGGCAGGGTCAGTGGTGCCACTTGACGTTGGCACAGCCACACAGTTGGACATCGTCGCAGAGTTTGAGTTGGATCAGACGGTTTTAGAGAGCACAGCTGAATCTAACGAGGAATTCAGTTGCCAAACTAGTGGTGGAGCTGGCAAACGCGGCGCATTGGGACCCTTTGGTCTTCTTGTTCTTGCGGATGACGGCCTCACTGAGCGCACTCCTGTCTACTTTTATGTTGTCAAGGGATCTGGTGGCACTGTCAATACCTACTTCTGTGCTGATCAAACAAGGTCTACTGTGGCAAATGATGTTGTTAAACAAGTTAGTGGTAGCTATGTTCCAGTGCTGAAAGGTGAAAAGCTGTCTGTGAGAATACTGGTTGATCATTCGATCATTGACAGCTTTGCTCAAGGTGGACGAACAACGATAACAACGCGCGTTTACCCAACTCAAGCAATTTATGGAGCTGCAAGGCTTTTCTTGTTCAACAATGCTACTGAGACCAGTGTTACTGCCTCACTCCAGGTATGGCAGATGAACTCTGCCTTCATACGCCCATTTTCACCTGATGCAGCAAGCCATGCAAGCTTCACCCCTGTCACAATATTCATCAATTTCATCGTCCCATTCGGACTATTTTTAACTTTATATTTTGTAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0004553 GO:0004564 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0007275 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009739 GO:0009987 GO:0010033 GO:0016787 GO:0016798 GO:0022622 GO:0030312 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048731 GO:0048856 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071944 GO:0080022 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00052 ko00500 ko01100 map00052 map00500 map01100

Pfam Domains

Protein Families

DUF3357 Glyco_hydro_32C Glyco_hydro_32N

Protein Analysis

695

Amino Acids

76.95

Weight (kDa)

5.9

Isoelectric Point (pI)

34.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
INV_N	PF11837	23 - 132	7.6e-16	Beta-fructofuranosidase, N-terminal domain
Glyco_hydro_32N	PF00251	140 - 458	1.4e-105	Glycosyl hydrolases family 32 N-terminal domain
Glyco_hydro_32C	PF08244	461 - 652	8.7e-22	Glycosyl hydrolases family 32 C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12240 AT1G62660 AT1G62660 AT1G62660 AT1G62660
fragaria_vesca	FvH4_5g00290 FvH4_5g00290 FvH4_5g00290
malus_domestica	MD06G1066600.v1.1
prunus_persica	Prupe.5G075600_v2.0.a1 Prupe.5G075600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0202311
rosa_laevigata	RLG00000003612
rosa_multiflora	Rmu_sc0010249.1_g000001
rosa_roxburghii	Rroxscaffold_3G00254670
rosa_rugosa	Rorug07G0069800
rosa_samantha	Rh7AG196900 Rh7BG198200 Rh7CG208100 Rh7DG203600
rosa_wichuraiana	Rw7G017260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1119
AasI	GACNNNNNNGTC	1 cut(s)	1599
Acc65I	GGTACC	1 cut(s)	480
AccB1I	GGYRCC	2 cut(s)	480, 1447
AccB7I	CCANNNNNTGG	1 cut(s)	834
AccI	GTMKAC	2 cut(s)	1647, 1715
AccII	CGCG	3 cut(s)	1581, 1858, 1860
AccIII	TCCGGA	1 cut(s)	76
AciI	CCGC	7 cut(s)	88, 290, 308, 348, 831, 1581, 1616
AcoI	YGGCCR	3 cut(s)	309, 655, 1370
AcsI	RAATTY	3 cut(s)	1196, 1317, 1544
AcuI	CTGAAG	1 cut(s)	1295
AcvI	CACGTG	2 cut(s)	97, 299
AdeI	CACNNNGTG	1 cut(s)	753
AfaI	GTAC	7 cut(s)	341, 482, 500, 764, 839, 970, 1425
AfiI	CCNNNNNNNGG	5 cut(s)	794, 795, 800, 834, 971
AflIII	ACRYGT	1 cut(s)	94
AgsI	TTSAA	4 cut(s)	684, 1367, 1402, 1909
AhlI	ACTAGT	2 cut(s)	1013, 1559
AjnI	CCWGG	4 cut(s)	108, 699, 964, 1944
AleI	CACNNNNGTG	1 cut(s)	368
AloI	GAACNNNNNNTCC	2 cut(s)	1125, 1157
AluBI	AGCT	9 cut(s)	596, 940, 1529, 1571, 1755, 1783, 1824, 1889, 2010
AluI	AGCT	9 cut(s)	596, 940, 1529, 1571, 1755, 1783, 1824, 1889, 2010
Alw21I	GWGCWC	1 cut(s)	1526
Alw26I	GTCTC	3 cut(s)	254, 549, 1916
Ama87I	CYCGRG	1 cut(s)	794
Aor13HI	TCCGGA	1 cut(s)	76
AoxI	GGCC	6 cut(s)	14, 183, 309, 655, 1370, 1624
ApeKI	GCWGC	4 cut(s)	345, 937, 1889, 1994
ApoI	RAATTY	3 cut(s)	1196, 1317, 1544
ArsI	GACNNNNNNTTYG	2 cut(s)	229, 261
Asp700I	GAANNNNTTC	1 cut(s)	1412
Asp718I	GGTACC	1 cut(s)	480
AspLEI	GCGC	3 cut(s)	1586, 1638, 1860
AspS9I	GGNCC	6 cut(s)	106, 458, 703, 820, 873, 1592
AsuC2I	CCSGG	6 cut(s)	124, 187, 776, 795, 796, 818
AsuHPI	GGTGA	5 cut(s)	566, 739, 1787, 1977, 2005
AvaI	CYCGRG	1 cut(s)	794
AvaII	GGWCC	6 cut(s)	106, 458, 703, 820, 873, 1592
BalI	TGGCCA	2 cut(s)	657, 1372
BamHI	GGATCC	1 cut(s)	62
BanI	GGYRCC	2 cut(s)	480, 1447
BbrPI	CACGTG	2 cut(s)	97, 299
BbsI	GAAGAC	2 cut(s)	237, 1595
Bbv12I	GWGCWC	1 cut(s)	1526
BbvI	GCAGC	4 cut(s)	357, 949, 1876, 2006
BccI	CCATC	3 cut(s)	127, 938, 1248
BceAI	ACGGC	1 cut(s)	1639
BcgI	CGANNNNNNTGC	4 cut(s)	20, 54, 334, 368
BciT130I	CCWGG	4 cut(s)	110, 701, 966, 1946
BciVI	GTATCC	3 cut(s)	84, 384, 559
BclI	TGATCA	6 cut(s)	175, 598, 1222, 1339, 1702, 1804
BcnI	CCSGG	6 cut(s)	124, 187, 776, 795, 796, 818
BcoDI	GTCTC	3 cut(s)	254, 549, 1916
BcuI	ACTAGT	2 cut(s)	1013, 1559
BfaI	CTAG	4 cut(s)	584, 1014, 1074, 1560
BfuI	GTATCC	3 cut(s)	84, 384, 559
BisI	GCNGC	7 cut(s)	309, 346, 349, 938, 1582, 1890, 1995
BlsI	GCNGC	7 cut(s)	310, 347, 350, 939, 1583, 1891, 1996
BmcAI	AGTACT	1 cut(s)	764
Bme18I	GGWCC	6 cut(s)	106, 458, 703, 820, 873, 1592
BmeT110I	CYCGRG	1 cut(s)	794
BmgT120I	GGNCC	6 cut(s)	106, 458, 703, 820, 873, 1592
BmiI	GGNNCC	9 cut(s)	64, 482, 821, 822, 874, 1143, 1449, 1593, 1594
BmsI	GCATC	3 cut(s)	28, 1313, 1981
BpiI	GAAGAC	2 cut(s)	237, 1595
BpmI	CTGGAG	1 cut(s)	1928
BpuEI	CTTGAG	2 cut(s)	1815, 1857
BpuMI	CCSGG	6 cut(s)	124, 187, 776, 795, 796, 818
BsaAI	YACGTR	2 cut(s)	97, 299
BsaBI	GATNNNNATC	3 cut(s)	803, 866, 996
BsaI	GGTCTC	1 cut(s)	1916
BsaJI	CCNNGG	5 cut(s)	123, 377, 794, 876, 915
BsaWI	WCCGGW	3 cut(s)	76, 204, 1000
Bsc4I	CCNNNNNNNGG	5 cut(s)	794, 795, 800, 834, 971
Bse118I	RCCGGY	2 cut(s)	333, 367
Bse3DI	GCAATG	1 cut(s)	594
Bse8I	GATNNNNATC	3 cut(s)	803, 866, 996
BseAI	TCCGGA	1 cut(s)	76
BseBI	CCWGG	4 cut(s)	110, 701, 966, 1946
BseDI	CCNNGG	5 cut(s)	123, 377, 794, 876, 915
BseGI	GGATG	3 cut(s)	447, 1259, 1624
BseJI	GATNNNNATC	3 cut(s)	803, 866, 996
BseLI	CCNNNNNNNGG	5 cut(s)	794, 795, 800, 834, 971
BseMI	GCAATG	1 cut(s)	594
BseMII	CTCAG	3 cut(s)	1029, 1623, 1911
BseRI	GAGGAG	4 cut(s)	58, 61, 734, 1394
BseX3I	CGGCCG	1 cut(s)	309
BseXI	GCAGC	4 cut(s)	357, 949, 1876, 2006
BsgI	GTGCAG	1 cut(s)	534
Bsh1236I	CGCG	3 cut(s)	1581, 1858, 1860
Bsh1285I	CGRYCG	1 cut(s)	312
BshFI	GGCC	6 cut(s)	16, 185, 311, 657, 1372, 1626
BshNI	GGYRCC	2 cut(s)	480, 1447
BsiEI	CGRYCG	1 cut(s)	312
BsiHKAI	GWGCWC	1 cut(s)	1526
BsiHKCI	CYCGRG	1 cut(s)	794
BslFI	GGGAC	3 cut(s)	833, 1605, 2030
BslI	CCNNNNNNNGG	5 cut(s)	794, 795, 800, 834, 971
BsmAI	GTCTC	3 cut(s)	254, 549, 1916
BsmFI	GGGAC	3 cut(s)	833, 1605, 2030
BsmI	GAATGC	2 cut(s)	196, 989
BsnI	GGCC	6 cut(s)	16, 185, 311, 657, 1372, 1626
Bso31I	GGTCTC	1 cut(s)	1916
BsoBI	CYCGRG	1 cut(s)	794
Bsp1286I	GDGCHC	1 cut(s)	1526
Bsp13I	TCCGGA	1 cut(s)	76
Bsp1407I	TGTACA	1 cut(s)	339
BspACI	CCGC	7 cut(s)	88, 290, 308, 348, 831, 1581, 1616
BspANI	GGCC	6 cut(s)	16, 185, 311, 657, 1372, 1626
BspCNI	CTCAG	3 cut(s)	1030, 1624, 1912
BspEI	TCCGGA	1 cut(s)	76
BspFNI	CGCG	3 cut(s)	1581, 1858, 1860
BspHI	TCATGA	1 cut(s)	172
BspLI	GGNNCC	9 cut(s)	64, 482, 821, 822, 874, 1143, 1449, 1593, 1594
BspT107I	GGYRCC	2 cut(s)	480, 1447
BspTNI	GGTCTC	1 cut(s)	1916
BsrDI	GCAATG	1 cut(s)	594
BsrFI	RCCGGY	2 cut(s)	333, 367
BsrGI	TGTACA	1 cut(s)	339
BssAI	RCCGGY	2 cut(s)	333, 367
BssECI	CCNNGG	5 cut(s)	123, 377, 794, 876, 915
BssT1I	CCWWGG	2 cut(s)	876, 915
Bst2UI	CCWGG	4 cut(s)	110, 701, 966, 1946
Bst4CI	ACNGT	6 cut(s)	458, 1330, 1476, 1513, 1681, 1720
Bst6I	CTCTTC	1 cut(s)	1314
BstAPI	GCANNNNNTGC	2 cut(s)	722, 2003
BstAUI	TGTACA	1 cut(s)	339
BstBAI	YACGTR	2 cut(s)	97, 299
BstC8I	GCNNGC	7 cut(s)	196, 288, 361, 365, 1573, 1999, 2008
BstDEI	CTNAG	3 cut(s)	1038, 1632, 1920
BstDSI	CCRYGG	1 cut(s)	377
BstF5I	GGATG	3 cut(s)	447, 1259, 1624
BstFNI	CGCG	3 cut(s)	1581, 1858, 1860
BstHHI	GCGC	3 cut(s)	1586, 1638, 1860
BstMAI	GTCTC	3 cut(s)	254, 549, 1916
BstMCI	CGRYCG	1 cut(s)	312
BstMWI	GCNNNNNNNGC	8 cut(s)	119, 191, 223, 593, 722, 1581, 2003, 2007
BstNI	CCWGG	4 cut(s)	110, 701, 966, 1946
BstNSI	RCATGY	3 cut(s)	217, 394, 1064
BstUI	CGCG	3 cut(s)	1581, 1858, 1860
BstV1I	GCAGC	4 cut(s)	357, 949, 1876, 2006
BstV2I	GAAGAC	2 cut(s)	237, 1595
BstX2I	RGATCY	3 cut(s)	62, 81, 1667
BstXI	CCANNNNNNTGG	2 cut(s)	1477, 1562
BstYI	RGATCY	3 cut(s)	62, 81, 1667
BstZI	CGGCCG	1 cut(s)	309
BsuI	GTATCC	3 cut(s)	84, 384, 559
BsuRI	GGCC	6 cut(s)	16, 185, 311, 657, 1372, 1626
BtgI	CCRYGG	1 cut(s)	377
BtgZI	GCGATG	1 cut(s)	128
BtsCI	GGATG	3 cut(s)	447, 1259, 1624
BtsIMutI	CAGTG	8 cut(s)	675, 1335, 1380, 1449, 1629, 1677, 1770, 1933
Cac8I	GCNNGC	7 cut(s)	196, 288, 361, 365, 1573, 1999, 2008
CciI	TCATGA	1 cut(s)	172
CfoI	GCGC	3 cut(s)	1586, 1638, 1860
Cfr10I	RCCGGY	2 cut(s)	333, 367
Cfr13I	GGNCC	6 cut(s)	106, 458, 703, 820, 873, 1592
Cfr9I	CCCGGG	1 cut(s)	794
Csp6I	GTAC	7 cut(s)	340, 481, 499, 763, 838, 969, 1424
CviAII	CATG	9 cut(s)	173, 214, 304, 391, 542, 667, 956, 1061, 2003
CviQI	GTAC	7 cut(s)	340, 481, 499, 763, 838, 969, 1424
DdeI	CTNAG	3 cut(s)	1038, 1632, 1920
DraIII	CACNNNGTG	1 cut(s)	753
DrdI	GACNNNNNNGTC	1 cut(s)	1599
DseDI	GACNNNNNNGTC	1 cut(s)	1599
EaeI	YGGCCR	3 cut(s)	309, 655, 1370
EagI	CGGCCG	1 cut(s)	309
Eam1104I	CTCTTC	1 cut(s)	1314
EarI	CTCTTC	1 cut(s)	1314
EciI	GGCGGA	1 cut(s)	103
EclXI	CGGCCG	1 cut(s)	309
Eco130I	CCWWGG	2 cut(s)	876, 915
Eco147I	AGGCCT	1 cut(s)	16
Eco31I	GGTCTC	1 cut(s)	1916
Eco47I	GGWCC	6 cut(s)	106, 458, 703, 820, 873, 1592
Eco52I	CGGCCG	1 cut(s)	309
Eco57I	CTGAAG	1 cut(s)	1295
Eco72I	CACGTG	2 cut(s)	97, 299
Eco88I	CYCGRG	1 cut(s)	794
EcoO109I	RGGNCCY	3 cut(s)	106, 820, 1592
EcoRI	GAATTC	2 cut(s)	1317, 1544
EcoRII	CCWGG	4 cut(s)	108, 699, 964, 1944
EcoT14I	CCWWGG	2 cut(s)	876, 915
EcoT22I	ATGCAT	2 cut(s)	989, 1108
ErhI	CCWWGG	2 cut(s)	876, 915
FaeI	CATG	9 cut(s)	176, 217, 307, 394, 545, 670, 959, 1064, 2006
FaqI	GGGAC	3 cut(s)	833, 1605, 2030
FatI	CATG	9 cut(s)	172, 213, 303, 390, 541, 666, 955, 1060, 2002
FbaI	TGATCA	6 cut(s)	175, 598, 1222, 1339, 1702, 1804
FblI	GTMKAC	2 cut(s)	1647, 1715
Fnu4HI	GCNGC	7 cut(s)	309, 346, 349, 938, 1582, 1890, 1995
FokI	GGATG	3 cut(s)	454, 1266, 1631
Fsp4HI	GCNGC	7 cut(s)	309, 346, 349, 938, 1582, 1890, 1995
FspBI	CTAG	4 cut(s)	584, 1014, 1074, 1560
GlaI	GCGC	3 cut(s)	1585, 1637, 1859
GluI	GCNGC	7 cut(s)	309, 346, 349, 938, 1582, 1890, 1995
GsuI	CTGGAG	1 cut(s)	1928
HaeIII	GGCC	6 cut(s)	16, 185, 311, 657, 1372, 1626
HhaI	GCGC	3 cut(s)	1586, 1638, 1860
Hin1II	CATG	9 cut(s)	176, 217, 307, 394, 545, 670, 959, 1064, 2006
Hin6I	GCGC	3 cut(s)	1584, 1636, 1858
HinP1I	GCGC	3 cut(s)	1584, 1636, 1858
HincII	GTYRAC	1 cut(s)	883
HindII	GTYRAC	1 cut(s)	883
HindIII	AAGCTT	1 cut(s)	2008
HinfI	GANTC	5 cut(s)	621, 692, 804, 1265, 1532
HpaI	GTTAAC	1 cut(s)	883
HphI	GGTGA	5 cut(s)	566, 739, 1787, 1977, 2005
Hpy166II	GTNNAC	6 cut(s)	840, 883, 1648, 1716, 1838, 1864
Hpy188I	TCNGA	8 cut(s)	31, 322, 803, 1039, 1227, 1270, 1509, 2054
Hpy188III	TCNNGA	7 cut(s)	77, 173, 179, 552, 625, 1145, 1291
Hpy8I	GTNNAC	6 cut(s)	840, 883, 1648, 1716, 1838, 1864
Hpy99I	CGWCG	1 cut(s)	1490
HpyCH4III	ACNGT	6 cut(s)	458, 1330, 1476, 1513, 1681, 1720
HpyCH4IV	ACGT	3 cut(s)	96, 298, 1458
HpyCH4V	TGCA	9 cut(s)	286, 515, 545, 716, 987, 1106, 1892, 1994, 2006
HpyF10VI	GCNNNNNNNGC	8 cut(s)	119, 191, 223, 593, 722, 1581, 2003, 2007
HpyF3I	CTNAG	3 cut(s)	1038, 1632, 1920
HpySE526I	ACGT	3 cut(s)	96, 298, 1458
Hsp92II	CATG	9 cut(s)	176, 217, 307, 394, 545, 670, 959, 1064, 2006
HspAI	GCGC	3 cut(s)	1584, 1636, 1858
KflI	GGGWCCC	2 cut(s)	820, 1592
Kpn2I	TCCGGA	1 cut(s)	76
KpnI	GGTACC	1 cut(s)	484
Ksp22I	TGATCA	6 cut(s)	175, 598, 1222, 1339, 1702, 1804
KspAI	GTTAAC	1 cut(s)	883
LmnI	GCTCC	2 cut(s)	1568, 1886
Lsp1109I	GCAGC	4 cut(s)	357, 949, 1876, 2006
LweI	GCATC	3 cut(s)	28, 1313, 1981
MaeI	CTAG	4 cut(s)	584, 1014, 1074, 1560
MaeII	ACGT	3 cut(s)	96, 298, 1458
MaeIII	GTNAC	4 cut(s)	294, 1016, 1930, 2020
MboII	GAAGA	4 cut(s)	237, 445, 1301, 1595
MflI	RGATCY	3 cut(s)	62, 81, 1667
MhlI	GDGCHC	1 cut(s)	1526
MlsI	TGGCCA	2 cut(s)	657, 1372
MluCI	AATT	9 cut(s)	436, 642, 660, 1196, 1317, 1391, 1544, 1878, 2035
MluNI	TGGCCA	2 cut(s)	657, 1372
MlyI	GAGTC	3 cut(s)	630, 701, 1274
MmeI	TCCRAC	4 cut(s)	54, 300, 1458, 1482
Mox20I	TGGCCA	2 cut(s)	657, 1372
Mph1103I	ATGCAT	2 cut(s)	989, 1108
MroI	TCCGGA	1 cut(s)	76
MroXI	GAANNNNTTC	1 cut(s)	1412
MscI	TGGCCA	2 cut(s)	657, 1372
MseI	TTAA	4 cut(s)	882, 927, 1737, 2063
MslI	CAYNNNNRTG	1 cut(s)	368
Msp20I	TGGCCA	2 cut(s)	657, 1372
MspA1I	CMGCKG	2 cut(s)	348, 1529
Mva1269I	GAATGC	2 cut(s)	196, 989
MvaI	CCWGG	4 cut(s)	110, 701, 966, 1946
MvnI	CGCG	3 cut(s)	1581, 1858, 1860
MwoI	GCNNNNNNNGC	8 cut(s)	119, 191, 223, 593, 722, 1581, 2003, 2007
NciI	CCSGG	6 cut(s)	124, 187, 776, 795, 796, 818
NlaIII	CATG	9 cut(s)	176, 217, 307, 394, 545, 670, 959, 1064, 2006
NlaIV	GGNNCC	9 cut(s)	64, 482, 821, 822, 874, 1143, 1449, 1593, 1594
NmuCI	GTSAC	3 cut(s)	294, 1016, 2020
NsiI	ATGCAT	2 cut(s)	989, 1108
NspI	RCATGY	3 cut(s)	217, 394, 1064
OliI	CACNNNNGTG	1 cut(s)	368
PagI	TCATGA	1 cut(s)	172
PceI	AGGCCT	1 cut(s)	16
PcsI	WCGNNNNNNNCGW	2 cut(s)	248, 326
PctI	GAATGC	2 cut(s)	196, 989
PdmI	GAANNNNTTC	1 cut(s)	1412
PfeI	GAWTC	2 cut(s)	804, 1532
PflFI	GACNNNGTC	2 cut(s)	241, 1484
PflMI	CCANNNNNTGG	1 cut(s)	834
PfoI	TCCNGGA	1 cut(s)	816
PkrI	GCNGC	7 cut(s)	310, 347, 350, 939, 1583, 1891, 1996
PleI	GAGTC	3 cut(s)	629, 700, 1273
PmaCI	CACGTG	2 cut(s)	97, 299
PmlI	CACGTG	2 cut(s)	97, 299
PpsI	GAGTC	3 cut(s)	629, 700, 1273
Ppu21I	YACGTR	2 cut(s)	97, 299
PpuMI	RGGWCCY	3 cut(s)	106, 820, 1592
PsiI	TTATAA	1 cut(s)	1119
Psp5II	RGGWCCY	3 cut(s)	106, 820, 1592
Psp6I	CCWGG	4 cut(s)	108, 699, 964, 1944
PspCI	CACGTG	2 cut(s)	97, 299
PspGI	CCWGG	4 cut(s)	108, 699, 964, 1944
PspN4I	GGNNCC	9 cut(s)	64, 482, 821, 822, 874, 1143, 1449, 1593, 1594
PspPI	GGNCC	6 cut(s)	106, 458, 703, 820, 873, 1592
PspPPI	RGGWCCY	3 cut(s)	106, 820, 1592
PsuI	RGATCY	3 cut(s)	62, 81, 1667
PsyI	GACNNNGTC	2 cut(s)	241, 1484
PvuII	CAGCTG	1 cut(s)	1529
RsaI	GTAC	7 cut(s)	341, 482, 500, 764, 839, 970, 1425
RsaNI	GTAC	7 cut(s)	340, 481, 499, 763, 838, 969, 1424
RseI	CAYNNNNRTG	1 cut(s)	368
SaqAI	TTAA	4 cut(s)	882, 927, 1737, 2063
SatI	GCNGC	7 cut(s)	309, 346, 349, 938, 1582, 1890, 1995
Sau96I	GGNCC	6 cut(s)	106, 458, 703, 820, 873, 1592
ScaI	AGTACT	1 cut(s)	764
SchI	GAGTC	3 cut(s)	630, 701, 1274
SduI	GDGCHC	1 cut(s)	1526
SfaNI	GCATC	3 cut(s)	28, 1313, 1981
SgrAI	CRCCGGYG	1 cut(s)	367
SinI	GGWCC	6 cut(s)	106, 458, 703, 820, 873, 1592
SmaI	CCCGGG	1 cut(s)	796
SmiMI	CAYNNNNRTG	1 cut(s)	368
SmlI	CTYRAG	2 cut(s)	1830, 1872
SmoI	CTYRAG	2 cut(s)	1830, 1872
SpeI	ACTAGT	2 cut(s)	1013, 1559
Sse9I	AATT	9 cut(s)	436, 642, 660, 1196, 1317, 1391, 1544, 1878, 2035
SseBI	AGGCCT	1 cut(s)	16
SsiI	CCGC	7 cut(s)	88, 290, 308, 348, 831, 1581, 1616
SspI	AATATT	1 cut(s)	2028
SspMI	CTAG	4 cut(s)	584, 1014, 1074, 1560
StuI	AGGCCT	1 cut(s)	16
StyI	CCWWGG	2 cut(s)	876, 915
TaaI	ACNGT	6 cut(s)	458, 1330, 1476, 1513, 1681, 1720
TaiI	ACGT	3 cut(s)	99, 301, 1461
TaqI	TCGA	4 cut(s)	329, 1007, 1416, 1811
TasI	AATT	9 cut(s)	436, 642, 660, 1196, 1317, 1391, 1544, 1878, 2035
TatI	WGTACW	4 cut(s)	339, 498, 762, 1423
TauI	GCSGC	3 cut(s)	311, 351, 1584
TfiI	GAWTC	2 cut(s)	804, 1532
Tru1I	TTAA	4 cut(s)	882, 927, 1737, 2063
Tru9I	TTAA	4 cut(s)	882, 927, 1737, 2063
TscAI	CASTG	8 cut(s)	682, 1335, 1380, 1449, 1636, 1684, 1770, 1933
TseFI	GTSAC	3 cut(s)	294, 1016, 2020
TseI	GCWGC	4 cut(s)	345, 937, 1889, 1994
Tsp45I	GTSAC	3 cut(s)	294, 1016, 2020
TspDTI	ATGAA	7 cut(s)	458, 944, 1421, 1960, 1973, 2020, 2029
TspGWI	ACGGA	3 cut(s)	15, 366, 685
TspMI	CCCGGG	1 cut(s)	794
TspRI	CASTG	8 cut(s)	682, 1335, 1380, 1449, 1636, 1684, 1770, 1933
Tth111I	GACNNNGTC	2 cut(s)	241, 1484
Van91I	CCANNNNNTGG	1 cut(s)	834
VpaK11BI	GGWCC	6 cut(s)	106, 458, 703, 820, 873, 1592
XapI	RAATTY	3 cut(s)	1196, 1317, 1544
XceI	RCATGY	3 cut(s)	217, 394, 1064
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1562
XmaI	CCCGGG	1 cut(s)	794
XmiI	GTMKAC	2 cut(s)	1647, 1715
XmnI	GAANNNNTTC	1 cut(s)	1412
XspI	CTAG	4 cut(s)	584, 1014, 1074, 1560
ZrmI	AGTACT	1 cut(s)	764
Zsp2I	ATGCAT	2 cut(s)	989, 1108

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.