AT1G12850

Phosphoglycerate mutase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012251
Physical Location & Seq
Reverse (-)
4379694 .. 4381764
2071 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G12850.1

Sequence Viewer

Length: 1218 bp
ATGGGGTCATCACAGTCTTCGCAATTACTCGATGAAGAAGAAGAAGAAGAAGATGAAGCAGAGAGCGAAGGTGAAGAAGTAGAAGAAGAAGAAGATGAAGCAGAGAGCGAATTGAATAACAGAAGAATCGAGCTGGATAATCTCTTGGTCAAGAAAGTTCTCGAGCAAGAGCCTGAGATGCTTCCTTGTCACGCCTCTGCTTCTCCACTCTCTCCTCAGCTCTCTTCTCTTGGAACTCCTCGAATCGGACCTTCCATTAAAGTCTGGGATCCTTACAACGTCCTCGCGCCACCTCCTCCTTCTTCTCCGCCTCTTTTCTCTCGTATCTCCTCCGCCGCGGAACACGATCGCTCCGCCGTGACGGAAGTCTATTTCATCAGCCACGGCGAGTGTGATCTCAATCTCAGGCCTGATCTGATTGGAGGTAGATGCCACGTGGCTACTCTCACACCCAACGGGAAACGTCAGGCGAGAGCTCTAGCCGTATTTTTAAACTCTGAAGGTGTTCGATTCACCTCCGTCTTCTCTTCGCCTCTGGATCGAGCTAGATCCATGGCTGTTTCTGTTTGCCAGGAAATGAATTTTCCTGAGGAGCATTTGCAAGTCTCGGATGCTGTGGTTGAGATGAGTTTAGGGGACTGGGAAAGCTGTCATCGGTCAGAGATTTACACACCTGAAACTCTAAGTTTAATAGAAAGATGCCAACCTGATTTCTCAGCTCCATCTGGTGAATCACTCAGACAAGTAGAGTTTCGGATGGTTCAGTTTCTGAATGGGACAGTCTCAGGACTTTCGGAGAAGCTCAGGTCAGAACTTCTTCCATCTACACAGCACACAAATTCCAGAGGGTTCTCGTTAGCTACTTCTATTCATCGCCCAATTCTTACAAGGAAGAAATCTGGGAAGAGCCGGTTTCAGGTGATGAATGCAACTGGTGATCACGAGGGTAGTGAAGAGATATTTAGTAATCACAATGATGAACAACACCTAGGTGATATAAACATCAAGAGTTCTTCTTCTCAACTCTCAACCTGCATTGGAGTTTTCACTCACTCTTTACCTATAAAGTGTCTTCTTACCGGTATCCTTGGATGCAGCCCGGTAATGACACATAAGATCTGTGTGGAGGATTCCTCTGTGACCGTATTACAGCATTCGTGGAAAACCGGGTGGCAGGTAAAGCGGTTAAATGACACCGCTCATCTTAGACTGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

405

Amino Acids

44.86

Weight (kDa)

5.03

Isoelectric Point (pI)

68.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 123 - 262 5.4e-20 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12850 AT3G26780
fragaria_vesca FvH4_6g54210
malus_domestica MD09G1004000.v1.1 MD17G1007700.v1.1
prunus_persica Prupe.3G312600_v2.0.a1 Prupe.3G312600_v2.0.a1
pyrus_communis pycom111g00300 pycom17g00320
rosa_chinensis RchiOBHm_Chr2g0176401
rosa_laevigata RLG00000022410
rosa_multiflora Rmu_co8476031.1_g000001
rosa_roxburghii Rroxscaffold_2G00076490
rosa_rugosa Rorug02G0593900
rosa_samantha Rh2AG672400 Rh2BG683900 Rh2CG646600 Rh2DG697400
rosa_wichuraiana Rw2G055150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1040, 1165
AccBSI CCGCTC 1 cut(s) 1199
AccII CGCG 2 cut(s) 287, 338
AciI CCGC 7 cut(s) 308, 333, 336, 338, 354, 1183, 1197
AclWI GGATC 4 cut(s) 263, 276, 543, 546
AcsI RAATTY 2 cut(s) 580, 838
AcuI CTGAAG 1 cut(s) 519
AcvI CACGTG 1 cut(s) 436
AfiI CCNNNNNNNGG 2 cut(s) 245, 916
AgeI ACCGGT 1 cut(s) 1079
AgsI TTSAA 1 cut(s) 115
AjnI CCWGG 1 cut(s) 570
AleI CACNNNNGTG 1 cut(s) 990
AluBI AGCT 8 cut(s) 133, 220, 476, 545, 648, 719, 802, 860
AluI AGCT 8 cut(s) 133, 220, 476, 545, 648, 719, 802, 860
Alw21I GWGCWC 1 cut(s) 478
Alw26I GTCTC 2 cut(s) 610, 787
AlwI GGATC 4 cut(s) 263, 276, 543, 546
AlwNI CAGNNNCTG 1 cut(s) 769
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 1 cut(s) 407
ApeKI GCWGC 1 cut(s) 1095
ApoI RAATTY 2 cut(s) 580, 838
AsiGI ACCGGT 1 cut(s) 1079
Asp700I GAANNNNTTC 2 cut(s) 504, 816
AspA2I CCTAGG 1 cut(s) 988
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 1 cut(s) 248
AsuC2I CCSGG 2 cut(s) 1100, 1168
AsuHPI GGTGA 6 cut(s) 83, 505, 740, 931, 947, 1004
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 1 cut(s) 248
AvrII CCTAGG 1 cut(s) 988
AxyI CCTNAGG 1 cut(s) 588
BamHI GGATCC 1 cut(s) 268
BanII GRGCYC 1 cut(s) 478
BauI CACGAG 1 cut(s) 941
BbrPI CACGTG 1 cut(s) 436
BbsI GAAGAC 3 cut(s) 9, 514, 1064
Bbv12I GWGCWC 1 cut(s) 478
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 1 cut(s) 1107
BccI CCATC 3 cut(s) 730, 751, 829
BceAI ACGGC 3 cut(s) 341, 400, 467
BciT130I CCWGG 1 cut(s) 572
BciVI GTATCC 1 cut(s) 1094
BclI TGATCA 1 cut(s) 937
BcnI CCSGG 2 cut(s) 1100, 1168
BcoDI GTCTC 2 cut(s) 610, 787
BfaI CTAG 3 cut(s) 479, 546, 989
BfuAI ACCTGC 2 cut(s) 1040, 1165
BfuI GTATCC 1 cut(s) 1094
BglII AGATCT 1 cut(s) 1116
BisI GCNGC 2 cut(s) 336, 1096
BlnI CCTAGG 1 cut(s) 988
BlsI GCNGC 2 cut(s) 337, 1097
Bme1390I CCNGG 3 cut(s) 572, 1100, 1168
Bme18I GGWCC 1 cut(s) 248
BmeT110I CYCGRG 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 1 cut(s) 270
BmrFI CCNGG 3 cut(s) 572, 1100, 1168
BmrI ACTGGG 1 cut(s) 649
BmsI GCATC 5 cut(s) 168, 419, 601, 689, 1082
BmuI ACTGGG 1 cut(s) 649
BoxI GACNNNNGTC 1 cut(s) 365
BpiI GAAGAC 3 cut(s) 9, 514, 1064
BplI GAGNNNNNCTC 2 cut(s) 1118, 1150
Bpu10I CCTNAGC 2 cut(s) 216, 803
BpuMI CCSGG 2 cut(s) 1100, 1168
BsaAI YACGTR 1 cut(s) 436
BsaBI GATNNNNATC 1 cut(s) 399
BsaJI CCNNGG 5 cut(s) 336, 382, 552, 988, 1087
BsaWI WCCGGW 1 cut(s) 1079
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 2 cut(s) 245, 916
Bse118I RCCGGY 2 cut(s) 909, 1079
Bse1I ACTGG 2 cut(s) 644, 937
Bse21I CCTNAGG 1 cut(s) 588
Bse8I GATNNNNATC 1 cut(s) 399
BseBI CCWGG 1 cut(s) 572
BseDI CCNNGG 5 cut(s) 336, 382, 552, 988, 1087
BseGI GGATG 3 cut(s) 616, 762, 1097
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 2 cut(s) 245, 916
BseMII CTCAG 8 cut(s) 165, 230, 418, 579, 729, 751, 798, 817
BseNI ACTGG 2 cut(s) 644, 937
BseRI GAGGAG 5 cut(s) 204, 228, 285, 319, 605
BseXI GCAGC 1 cut(s) 1107
Bsh1236I CGCG 2 cut(s) 287, 338
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 1 cut(s) 409
BshTI ACCGGT 1 cut(s) 1079
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 478
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 4 cut(s) 910, 1080, 1100, 1167
BslFI GGGAC 2 cut(s) 650, 790
BslI CCNNNNNNNGG 2 cut(s) 245, 916
BsmAI GTCTC 2 cut(s) 610, 787
BsmFI GGGAC 2 cut(s) 650, 790
BsmI GAATGC 2 cut(s) 931, 1153
BsnI GGCC 1 cut(s) 409
BsoBI CYCGRG 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 478
Bsp143I GATC 8 cut(s) 268, 346, 394, 412, 538, 548, 937, 1116
Bsp19I CCATGG 1 cut(s) 552
BspACI CCGC 7 cut(s) 308, 333, 336, 338, 354, 1183, 1197
BspANI GGCC 1 cut(s) 409
BspCNI CTCAG 8 cut(s) 166, 229, 417, 580, 728, 750, 797, 816
BspFNI CGCG 2 cut(s) 287, 338
BspLI GGNNCC 1 cut(s) 270
BspMI ACCTGC 2 cut(s) 1040, 1165
BspPI GGATC 4 cut(s) 263, 276, 543, 546
BspQI GCTCTTC 1 cut(s) 899
BsrBI CCGCTC 1 cut(s) 1199
BsrFI RCCGGY 2 cut(s) 909, 1079
BsrI ACTGG 2 cut(s) 644, 937
BssAI RCCGGY 2 cut(s) 909, 1079
BssECI CCNNGG 5 cut(s) 336, 382, 552, 988, 1087
BssMI GATC 8 cut(s) 268, 346, 394, 412, 538, 548, 937, 1116
BssSI CACGAG 1 cut(s) 941
BssT1I CCWWGG 3 cut(s) 552, 988, 1087
Bst2BI CACGAG 1 cut(s) 941
Bst2UI CCWGG 1 cut(s) 572
Bst4CI ACNGT 4 cut(s) 15, 781, 1144, 1212
Bst6I CTCTTC 4 cut(s) 229, 532, 899, 948
BstBAI YACGTR 1 cut(s) 436
BstDSI CCRYGG 3 cut(s) 336, 382, 552
BstF5I GGATG 3 cut(s) 616, 762, 1097
BstFNI CGCG 2 cut(s) 287, 338
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 8 cut(s) 271, 349, 397, 415, 541, 551, 940, 1119
BstMAI GTCTC 2 cut(s) 610, 787
BstMBI GATC 8 cut(s) 268, 346, 394, 412, 538, 548, 937, 1116
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 2 cut(s) 178, 1180
BstNI CCWGG 1 cut(s) 572
BstPAI GACNNNNGTC 1 cut(s) 365
BstSCI CCNGG 3 cut(s) 570, 1098, 1166
BstUI CGCG 2 cut(s) 287, 338
BstV1I GCAGC 1 cut(s) 1107
BstV2I GAAGAC 3 cut(s) 9, 514, 1064
BstX2I RGATCY 3 cut(s) 268, 548, 1116
BstYI RGATCY 3 cut(s) 268, 548, 1116
Bsu36I CCTNAGG 1 cut(s) 588
BsuI GTATCC 1 cut(s) 1094
BsuRI GGCC 1 cut(s) 409
BtgI CCRYGG 3 cut(s) 336, 382, 552
BtgZI GCGATG 1 cut(s) 857
BtsCI GGATG 3 cut(s) 616, 762, 1097
BveI ACCTGC 2 cut(s) 1040, 1165
CaiI CAGNNNCTG 1 cut(s) 769
CfoI GCGC 1 cut(s) 289
Cfr10I RCCGGY 2 cut(s) 909, 1079
Cfr13I GGNCC 1 cut(s) 248
Cfr42I CCGCGG 1 cut(s) 339
CspAI ACCGGT 1 cut(s) 1079
CviAII CATG 1 cut(s) 553
DpnI GATC 8 cut(s) 270, 348, 396, 414, 540, 550, 939, 1118
DpnII GATC 8 cut(s) 268, 346, 394, 412, 538, 548, 937, 1116
DraI TTTAAA 1 cut(s) 492
Eam1104I CTCTTC 4 cut(s) 229, 532, 899, 948
EarI CTCTTC 4 cut(s) 229, 532, 899, 948
EciI GGCGGA 3 cut(s) 297, 322, 343
Ecl136II GAGCTC 1 cut(s) 476
Eco130I CCWWGG 3 cut(s) 552, 988, 1087
Eco147I AGGCCT 1 cut(s) 409
Eco24I GRGCYC 1 cut(s) 478
Eco47I GGWCC 1 cut(s) 248
Eco53kI GAGCTC 1 cut(s) 476
Eco57I CTGAAG 1 cut(s) 519
Eco72I CACGTG 1 cut(s) 436
Eco81I CCTNAGG 1 cut(s) 588
Eco88I CYCGRG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 476
EcoRII CCWGG 1 cut(s) 570
EcoT14I CCWWGG 3 cut(s) 552, 988, 1087
EcoT38I GRGCYC 1 cut(s) 478
ErhI CCWWGG 3 cut(s) 552, 988, 1087
FaeI CATG 1 cut(s) 556
FaiI YATR 4 cut(s) 554, 998, 1064, 1113
FaqI GGGAC 2 cut(s) 650, 790
FatI CATG 1 cut(s) 552
FbaI TGATCA 1 cut(s) 937
Fnu4HI GCNGC 2 cut(s) 336, 1096
FokI GGATG 3 cut(s) 623, 769, 1104
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 2 cut(s) 336, 1096
FspBI CTAG 3 cut(s) 479, 546, 989
GlaI GCGC 1 cut(s) 288
GluI GCNGC 2 cut(s) 336, 1096
HaeIII GGCC 1 cut(s) 409
HapII CCGG 4 cut(s) 910, 1080, 1100, 1167
HhaI GCGC 1 cut(s) 289
Hin1II CATG 1 cut(s) 556
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HinfI GANTC 5 cut(s) 126, 243, 510, 731, 1130
HpaII CCGG 4 cut(s) 910, 1080, 1100, 1167
HphI GGTGA 6 cut(s) 83, 505, 740, 931, 947, 1004
Hpy188III TCNNGA 8 cut(s) 151, 161, 536, 587, 786, 843, 941, 1006
HpyAV CCTTC 4 cut(s) 62, 261, 309, 494
HpyCH4III ACNGT 4 cut(s) 15, 781, 1144, 1212
HpyCH4IV ACGT 3 cut(s) 279, 435, 463
HpyCH4V TGCA 4 cut(s) 601, 929, 1035, 1095
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 1180
HpySE526I ACGT 3 cut(s) 279, 435, 463
Hsp92II CATG 1 cut(s) 556
HspAI GCGC 1 cut(s) 287
Ksp22I TGATCA 1 cut(s) 937
KspI CCGCGG 1 cut(s) 339
Kzo9I GATC 8 cut(s) 268, 346, 394, 412, 538, 548, 937, 1116
LguI GCTCTTC 1 cut(s) 899
LmnI GCTCC 3 cut(s) 356, 592, 724
Lsp1109I GCAGC 1 cut(s) 1107
LweI GCATC 5 cut(s) 168, 419, 601, 689, 1082
MaeI CTAG 3 cut(s) 479, 546, 989
MaeII ACGT 3 cut(s) 279, 435, 463
MaeIII GTNAC 3 cut(s) 188, 358, 1138
MalI GATC 8 cut(s) 270, 348, 396, 414, 540, 550, 939, 1118
MbiI CCGCTC 1 cut(s) 1199
MboI GATC 8 cut(s) 268, 346, 394, 412, 538, 548, 937, 1116
MflI RGATCY 3 cut(s) 268, 548, 1116
MhlI GDGCHC 1 cut(s) 478
MluCI AATT 5 cut(s) 23, 110, 580, 838, 879
MroXI GAANNNNTTC 2 cut(s) 504, 816
MseI TTAA 4 cut(s) 258, 491, 689, 1187
MslI CAYNNNNRTG 2 cut(s) 975, 990
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 4 cut(s) 910, 1080, 1100, 1167
MspR9I CCNGG 3 cut(s) 572, 1100, 1168
Mva1269I GAATGC 2 cut(s) 931, 1153
MvaI CCWGG 1 cut(s) 572
MvnI CGCG 2 cut(s) 287, 338
MwoI GCNNNNNNNGC 2 cut(s) 178, 1180
NciI CCSGG 2 cut(s) 1100, 1168
NcoI CCATGG 1 cut(s) 552
NdeII GATC 8 cut(s) 268, 346, 394, 412, 538, 548, 937, 1116
NlaIII CATG 1 cut(s) 556
NlaIV GGNNCC 1 cut(s) 270
NmuCI GTSAC 3 cut(s) 188, 358, 1138
OliI CACNNNNGTG 1 cut(s) 990
PaeR7I CTCGAG 1 cut(s) 161
PceI AGGCCT 1 cut(s) 409
PciSI GCTCTTC 1 cut(s) 899
PctI GAATGC 2 cut(s) 931, 1153
PdmI GAANNNNTTC 2 cut(s) 504, 816
PfeI GAWTC 5 cut(s) 126, 243, 510, 731, 1130
PinAI ACCGGT 1 cut(s) 1079
PkrI GCNGC 2 cut(s) 337, 1097
Ple19I CGATCG 1 cut(s) 349
PmaCI CACGTG 1 cut(s) 436
PmlI CACGTG 1 cut(s) 436
Ppu21I YACGTR 1 cut(s) 436
PshAI GACNNNNGTC 1 cut(s) 365
Psp124BI GAGCTC 1 cut(s) 478
Psp6I CCWGG 1 cut(s) 570
PspCI CACGTG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 570
PspN4I GGNNCC 1 cut(s) 270
PspPI GGNCC 1 cut(s) 248
PstNI CAGNNNCTG 1 cut(s) 769
PsuI RGATCY 3 cut(s) 268, 548, 1116
PvuI CGATCG 1 cut(s) 349
RseI CAYNNNNRTG 2 cut(s) 975, 990
SacI GAGCTC 1 cut(s) 478
SacII CCGCGG 1 cut(s) 339
SapI GCTCTTC 1 cut(s) 899
SaqAI TTAA 4 cut(s) 258, 491, 689, 1187
SatI GCNGC 2 cut(s) 336, 1096
Sau3AI GATC 8 cut(s) 268, 346, 394, 412, 538, 548, 937, 1116
Sau96I GGNCC 1 cut(s) 248
ScrFI CCNGG 3 cut(s) 572, 1100, 1168
SduI GDGCHC 1 cut(s) 478
SfaNI GCATC 5 cut(s) 168, 419, 601, 689, 1082
Sfr274I CTCGAG 1 cut(s) 161
Sfr303I CCGCGG 1 cut(s) 339
SgrBI CCGCGG 1 cut(s) 339
SinI GGWCC 1 cut(s) 248
SlaI CTCGAG 1 cut(s) 161
SmiMI CAYNNNNRTG 2 cut(s) 975, 990
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
Sse9I AATT 5 cut(s) 23, 110, 580, 838, 879
SseBI AGGCCT 1 cut(s) 409
SsiI CCGC 7 cut(s) 308, 333, 336, 338, 354, 1183, 1197
SspMI CTAG 3 cut(s) 479, 546, 989
SstI GAGCTC 1 cut(s) 478
StuI AGGCCT 1 cut(s) 409
StyD4I CCNGG 3 cut(s) 570, 1098, 1166
StyI CCWWGG 3 cut(s) 552, 988, 1087
TaaI ACNGT 4 cut(s) 15, 781, 1144, 1212
TaiI ACGT 3 cut(s) 282, 438, 466
TaqI TCGA 6 cut(s) 30, 129, 162, 241, 508, 541
TaqII GACCGA 1 cut(s) 645
TasI AATT 5 cut(s) 23, 110, 580, 838, 879
TauI GCSGC 1 cut(s) 338
TfiI GAWTC 5 cut(s) 126, 243, 510, 731, 1130
Tru1I TTAA 4 cut(s) 258, 491, 689, 1187
Tru9I TTAA 4 cut(s) 258, 491, 689, 1187
TseFI GTSAC 3 cut(s) 188, 358, 1138
TseI GCWGC 1 cut(s) 1095
Tsp45I GTSAC 3 cut(s) 188, 358, 1138
TspDTI ATGAA 8 cut(s) 48, 69, 111, 364, 593, 860, 938, 993
TspGWI ACGGA 2 cut(s) 377, 508
VpaK11BI GGWCC 1 cut(s) 248
XapI RAATTY 2 cut(s) 580, 838
XhoI CTCGAG 1 cut(s) 161
XmaJI CCTAGG 1 cut(s) 988
XmnI GAANNNNTTC 2 cut(s) 504, 816
XspI CTAG 3 cut(s) 479, 546, 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.