Rorug02G0593900

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0012251
Physical Location & Seq
Forward (+)
71564036 .. 71571840
7805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0593900.1

Sequence Viewer

Length: 3156 bp
ATGGCTGCTTTAAGATCCTTCAACAATCTTCTAGCCCTTTCTCTATTCACCACCTTGTGTCTACTTATTACTCTTCCAGGGGCCAATGGTGATGATTGGTTCCAGTCGCATATTGCTACGCAGAACTGGTTAAACCACGGTGGTAATTTGTACAACACAAGATATGCCAGCAAGGAGACCAAGATCAGCCCTACAACGGTTTCGAACCTCCGCCTGAAGTGGAAGTTCTTTGCAGGTGACCATATAACTGCAACGCCGGCAACCTACGATGACAAGCTTTATTTTCCTAGCTGGAACGGTTACATCTACGCCGTCAAAGCGTCCGATGGATCCCTTATTTGGAAGAAGAATTTGCTAACCTTGACCGGCCTTAATACAACCGGTTTCGTTTTTGCCATCAACACTACGGTGTCCAGATCAACCCCGACGATTGCTGGTGATCATGATGAGCTGCTGATCATTGGACTGTATGCACCTGCGGTTGTTATTGCCGTGGAAAGGTCAACAGGGAAGCTTGTTTGGTCAACGTCTCTTGATAGCCACCCCGCAAGTATTATAACCATGTCAGGCACTTACTACAAAGGGAGTATTTATGTTGGAACGTCATCGCAAGAAGAATCTGTCACCGTTGAGAAATGTTGCACATTTCGTGGCAGTCTTGCTAAGTTGGATGTCAAATCCGGCGTTATTATGTGGAAAACTTTCATGTTGCCGGATAATCATGGAGACTTGGGACAATACTCCGGAGCTGCAATCTGGGGAAGTAGTCCATCCATCGATGTCTTCAGAAAACATGTCTATATTGCTACTGGAAACACGTATTCAGTCCCCGAAAATGTGAGTCTATGCCAAGAAAATCAGAACAATTCAACCTTGCCTACTCATTCAGATTCGTGCATTGAGCCCGACAACCATGGCAATTCAATCCTAGCACTTGATATGGACTCTGGCGAAATTAAATGGTACCACCAGTTGGGCGGCTACGATGTATGGTTTTTGCAATGTCTGTTTAATACTTCGACCTGCCCTCCTGGACCTAATGTGGATGCTGATTTTGGGGAAGCACCTATGATGCTTAGTGCACAAGTCAATGGGAGCAAGCTAGATTTAGTTGTTGCCGTTCAGAAAAGCGGGTTTGCATGGGCTCTAGATCGGAATAATGGCAGCCTTGTGTGGTCTACGCAAGCTGGGCCTAGTGGCATTGGTGGAGGAGGAATTTGGGGAGCAGCTACTGATGCGAAAAGGGTATACACCAACATTGCTAATTCTGGTGCCAAAAATTTCACTCTCAAGCCATCGAACAAGATAACGACTTCGGGTGGATGGGTGGCAATGGTCTCTGGCAGTGGCGAGATTCTATGGTCAACAGCGAATCCCAGCAATGCAACTTCTTATGGACCAGTTAGCGTAGCTAATGATGTCCTTTTTGGTGGATCCACAAGCCCAAAAGGGGAGATATACGCCATGGACGCCAAGACTGGCGAAGTTTTGTGGTCCTATGTCACCGGAGCAACCGTGTATGGTGGCATGTCCATAAGCAATGGTTGCATTTACATTGGCAATGGATATAGACCTCCATTTACTGCTGGAACCTCGTTGTTCGCCTTCTGTTGGCCTTTTGCAGAGAAATACAAGCCTAGCACTCAAGATTGGCCGAACCATGGAGGAGACTTGTATAACAGAAGATATGCTAACACGGAGAAGAAGATAAGCCCTGCAACAGTTTCCAATCTCAGTGTGAAGTGGAAATTCTCCGTGGGAGGTGACATAACTGTAACACCAACCATTTCTGATGGCATTCTCTACTTCCCTAGCTGGAATGGATATCTCTATGCCGTTAAAGAATCTGATGGATCCCTTGTCTGGAAGAAAAATGTGCAGAAGTTGACAGGCTTCAATAACACTGGCTTCATTCTCAATGTCAACTCCACAGTGACAAGATCAACCCCAACAATCGCCGGTGACCTTCTCATTGTTGGAATCTATGGTCCTGCTTTTGTCATTGCTGTCAAAAGGTCCAATGGGAAGCTCGTTTGGTCTACCAGGGTTGATAACCACACCAGAGCCTTTGTCACCATGTCTGGAACATATTACAATGGGGGATACTATGTTGGAACATCCTCTCTAGAAGAAGGCCTCACCATCGATCTGTGCTGCACATTTCGGGGGAGCTTCCTTAAATTAGATGTCCAAACAGGAGCAATCTTGTGGCAGACCTTCATGCTACCAGACAACTTTGGCAAGTTTGGAGAGTACGCTGGTGCAGCAATCTGGGGAAGTAGTCCTTCTATCGATGTGAAGCGTAACCATGTCTACATTGCCACTTCGAACCTGTATTCAGCTCCTCCGCGTATAATTGAATGCCAGGAACGGGAAAATAATCAAACCATACCTTCACAACCAGATGCCTGCATTGAGCCTGACAACCACTCCGAGTCTATTGTAGCCCTTGATTTAGACACTGGCAAAATCAAATGGTACAAGCAGCTGGGAGGCTATGATATATGGTTCGGTGCATGCCATCGGCATTTGGACCCAAGGTGCCCTCCCGGTCCAAGTCCAGACGCTGATTTTGGGGAAGCACCAATGATGCTTACCACAAAAATTAATGGGACTAAGAGAGATATAGTAGTTGCTGTTCAGAAAAGTGGGTTTGCATGGGCTCTGGACCGAGATACTGGTAGCTTAGTGTGGTCTACGGAAGCTGGACCAGGAGGCTTAGGAGGAGGAGCCATGTGGGGTGCAGCCACTGACGAAAAGAGGGTCTATACTAACATTGCCAACAGCCAACACAAGAACTTCACCCTAAAACCATCCCAGAACACTACAATTGCTGGTGGATGGGTAGCAATGGAGGCTCGCAGTGGCAATATCTTATGGTCCACAGCCAATGCTAATGATGCTACCGCCCCGGCTGCAGTTACTGTGGCTAATGGTGTTGTTTTTGGTGGCTCCACGTACCGGCAAGGACCAATATATGCAATGAATGCTAAGACAGGAAAGATTTTGTGGTCGTATGATACAGGAGCAACTGTGTATGGGGGGATTTCGGTAAGCAATGGATGCATTTACCTCGGAAATGGTTACAAGGTCTTTAATGGATTTGTGAACCCAAATTACACTGCAGGAACCTCGTTCTATGCCTTCTGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

1051

Amino Acids

113.39

Weight (kDa)

6.97

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQQ PF01011 43 - 395 1.9e-16 Glucose/ethanol/alcohol dehydrogenase, beta-propeller domain
PQQ_2 PF13360 74 - 321 9.3e-09 Outer membrane protein assembly factor BamB
PQQ_2 PF13360 440 - 518 9.7e-07 Outer membrane protein assembly factor BamB
PQQ_2 PF13360 582 - 826 1.4e-09 Outer membrane protein assembly factor BamB
PQQ PF01011 729 - 901 7.2e-12 Glucose/ethanol/alcohol dehydrogenase, beta-propeller domain
PQQ_2 PF13360 947 - 1025 1.3e-06 Outer membrane protein assembly factor BamB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12850 AT3G26780
fragaria_vesca FvH4_6g54210
malus_domestica MD09G1004000.v1.1 MD17G1007700.v1.1
prunus_persica Prupe.3G312600_v2.0.a1 Prupe.3G312600_v2.0.a1
pyrus_communis pycom111g00300 pycom17g00320
rosa_chinensis RchiOBHm_Chr2g0176401
rosa_laevigata RLG00000022410
rosa_multiflora Rmu_co8476031.1_g000001
rosa_roxburghii Rroxscaffold_2G00076490
rosa_rugosa Rorug02G0593900
rosa_samantha Rh2AG672400 Rh2BG683900 Rh2CG646600 Rh2DG697400
rosa_wichuraiana Rw2G055150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 557
AarI CACCTGC 2 cut(s) 224, 484
Acc36I ACCTGC 3 cut(s) 224, 484, 1031
Acc65I GGTACC 1 cut(s) 963
AccB1I GGYRCC 3 cut(s) 963, 1271, 2541
AccB7I CCANNNNNTGG 1 cut(s) 973
AccI GTMKAC 6 cut(s) 61, 1178, 1248, 2039, 2313, 2696
AccII CGCG 1 cut(s) 2350
AccIII TCCGGA 1 cut(s) 743
AciI CCGC 7 cut(s) 211, 479, 546, 978, 1131, 2348, 2907
AclWI GGATC 7 cut(s) 9, 324, 337, 1428, 1441, 1848, 1861
AcoI YGGCCR 1 cut(s) 1652
AcsI RAATTY 4 cut(s) 349, 1215, 1279, 1748
AcuI CTGAAG 2 cut(s) 236, 769
AcyI GRCGYC 1 cut(s) 1470
AdeI CACNNNGTG 1 cut(s) 57
AfaI GTAC 5 cut(s) 152, 965, 2255, 2480, 2960
AflIII ACRYGT 2 cut(s) 793, 816
AgeI ACCGGT 1 cut(s) 380
AgsI TTSAA 5 cut(s) 22, 870, 924, 1897, 2360
AjnI CCWGG 5 cut(s) 76, 1030, 2042, 2364, 2710
AleI CACNNNNGTG 1 cut(s) 407
Alw21I GWGCWC 1 cut(s) 1084
Alw26I GTCTC 5 cut(s) 170, 534, 720, 1342, 1662
Alw44I GTGCAC 1 cut(s) 1080
AlwI GGATC 7 cut(s) 9, 324, 337, 1428, 1441, 1848, 1861
AlwNI CAGNNNCTG 4 cut(s) 1232, 2567, 2750, 2924
Aor13HI TCCGGA 1 cut(s) 743
AoxI GGCC 6 cut(s) 81, 367, 1190, 1613, 1652, 2134
ApaLI GTGCAC 1 cut(s) 1080
ApoI RAATTY 4 cut(s) 349, 1215, 1279, 1748
ArsI GACNNNNNNTTYG 4 cut(s) 2555, 2587, 2748, 2780
AseI ATTAAT 1 cut(s) 2607
AsiGI ACCGGT 1 cut(s) 380
Asp700I GAANNNNTTC 1 cut(s) 701
Asp718I GGTACC 1 cut(s) 963
AsuC2I CCSGG 2 cut(s) 2550, 2912
AsuII TTCGAA 2 cut(s) 203, 2327
BaeGI GKGCMC 2 cut(s) 1084, 2546
BamHI GGATCC 3 cut(s) 329, 1433, 1853
BanI GGYRCC 3 cut(s) 963, 1271, 2541
BanII GRGCYC 3 cut(s) 906, 1147, 2665
BbsI GAAGAC 1 cut(s) 775
Bbv12I GWGCWC 1 cut(s) 1084
BbvI GCAGC 9 cut(s) 438, 736, 1176, 1238, 2141, 2276, 2497, 2756, 2902
BceAI ACGGC 4 cut(s) 296, 476, 1103, 1820
BcgI CGANNNNNNTGC 4 cut(s) 3055, 3089, 3114, 3148
BciT130I CCWGG 5 cut(s) 78, 1032, 2044, 2366, 2712
BciVI GTATCC 1 cut(s) 2096
BclI TGATCA 2 cut(s) 439, 456
BcnI CCSGG 2 cut(s) 2550, 2912
BcoDI GTCTC 5 cut(s) 170, 534, 720, 1342, 1662
BfaI CTAG 9 cut(s) 32, 288, 929, 1103, 1148, 1194, 1638, 1812, 2126
BfmI CTRYAG 2 cut(s) 2916, 3123
BfuAI ACCTGC 3 cut(s) 224, 484, 1031
BfuI GTATCC 1 cut(s) 2096
Bme1390I CCNGG 7 cut(s) 78, 1032, 2044, 2366, 2550, 2712, 2912
BmrFI CCNGG 7 cut(s) 78, 1032, 2044, 2366, 2550, 2712, 2912
BmsI GCATC 7 cut(s) 1036, 1062, 1225, 2395, 2580, 2890, 3053
BpiI GAAGAC 1 cut(s) 775
Bpu10I CCTNAGC 1 cut(s) 2719
Bpu14I TTCGAA 2 cut(s) 203, 2327
BpuEI CTTGAG 2 cut(s) 1274, 1629
BpuMI CCSGG 2 cut(s) 2550, 2912
Bsa29I ATCGAT 3 cut(s) 777, 2145, 2292
BsaAI YACGTR 2 cut(s) 819, 2958
BsaHI GRCGYC 1 cut(s) 1470
BsaI GGTCTC 2 cut(s) 170, 1342
BsaWI WCCGGW 3 cut(s) 380, 743, 1505
BsaXI ACNNNNNCTCC 8 cut(s) 1012, 1042, 1500, 1530, 2414, 2444, 3018, 3048
Bse118I RCCGGY 5 cut(s) 256, 365, 380, 1958, 2961
Bse1I ACTGG 9 cut(s) 103, 131, 814, 970, 1400, 1483, 1909, 2467, 2683
BseAI TCCGGA 1 cut(s) 743
BseBI CCWGG 5 cut(s) 78, 1032, 2044, 2366, 2712
BseCI ATCGAT 3 cut(s) 777, 2145, 2292
BseGI GGATG 8 cut(s) 676, 770, 1051, 1328, 2117, 2813, 2846, 3068
BseMII CTCAG 1 cut(s) 1747
BseNI ACTGG 9 cut(s) 103, 131, 814, 970, 1400, 1483, 1909, 2467, 2683
BseRI GAGGAG 5 cut(s) 1224, 1680, 2334, 2739, 2742
BseSI GKGCMC 2 cut(s) 1084, 2546
BseXI GCAGC 9 cut(s) 438, 736, 1176, 1238, 2141, 2276, 2497, 2756, 2902
BseYI CCCAGC 3 cut(s) 1187, 1376, 2488
BsgI GTGCAG 4 cut(s) 1898, 2140, 2283, 2763
Bsh1236I CGCG 1 cut(s) 2350
BshFI GGCC 6 cut(s) 83, 369, 1192, 1615, 1654, 2136
BshNI GGYRCC 3 cut(s) 963, 1271, 2541
BshTI ACCGGT 1 cut(s) 380
BshVI ATCGAT 3 cut(s) 777, 2145, 2292
BsiHKAI GWGCWC 1 cut(s) 1084
BslFI GGGAC 3 cut(s) 747, 812, 2626
BsmAI GTCTC 5 cut(s) 170, 534, 720, 1342, 1662
BsmBI CGTCTC 1 cut(s) 534
BsmFI GGGAC 3 cut(s) 747, 812, 2626
BsmI GAATGC 3 cut(s) 1797, 2366, 2992
BsnI GGCC 6 cut(s) 83, 369, 1192, 1615, 1654, 2136
Bso31I GGTCTC 2 cut(s) 170, 1342
Bsp119I TTCGAA 2 cut(s) 203, 2327
Bsp1286I GDGCHC 5 cut(s) 906, 1084, 1147, 2546, 2665
Bsp13I TCCGGA 1 cut(s) 743
Bsp1407I TGTACA 1 cut(s) 150
Bsp19I CCATGG 3 cut(s) 913, 1464, 1660
BspACI CCGC 7 cut(s) 211, 479, 546, 978, 1131, 2348, 2907
BspANI GGCC 6 cut(s) 83, 369, 1192, 1615, 1654, 2136
BspCNI CTCAG 1 cut(s) 1746
BspDI ATCGAT 3 cut(s) 777, 2145, 2292
BspEI TCCGGA 1 cut(s) 743
BspFNI CGCG 1 cut(s) 2350
BspHI TCATGA 1 cut(s) 442
BspMAI CTGCAG 2 cut(s) 2920, 3127
BspMI ACCTGC 3 cut(s) 224, 484, 1031
BspPI GGATC 7 cut(s) 9, 324, 337, 1428, 1441, 1848, 1861
BspT104I TTCGAA 2 cut(s) 203, 2327
BspT107I GGYRCC 3 cut(s) 963, 1271, 2541
BspTNI GGTCTC 2 cut(s) 170, 1342
BsrFI RCCGGY 5 cut(s) 256, 365, 380, 1958, 2961
BsrGI TGTACA 1 cut(s) 150
BsrI ACTGG 9 cut(s) 103, 131, 814, 970, 1400, 1483, 1909, 2467, 2683
BssAI RCCGGY 5 cut(s) 256, 365, 380, 1958, 2961
BssNAI GTATAC 1 cut(s) 1249
BssNI GRCGYC 1 cut(s) 1470
BssT1I CCWWGG 4 cut(s) 913, 1464, 1660, 2537
Bst1107I GTATAC 1 cut(s) 1249
Bst2UI CCWGG 5 cut(s) 78, 1032, 2044, 2366, 2712
Bst6I CTCTTC 1 cut(s) 78
BstACI GRCGYC 1 cut(s) 1470
BstAPI GCANNNNNTGC 3 cut(s) 1545, 2987, 3063
BstAUI TGTACA 1 cut(s) 150
BstBAI YACGTR 2 cut(s) 819, 2958
BstBI TTCGAA 2 cut(s) 203, 2327
BstC8I GCNNGC 7 cut(s) 169, 258, 1100, 1185, 2410, 2518, 2860
BstDEI CTNAG 7 cut(s) 663, 1076, 1733, 2616, 2686, 2719, 2991
BstDSI CCRYGG 6 cut(s) 136, 492, 913, 1464, 1660, 1755
BstEII GGTNACC 2 cut(s) 236, 1961
BstF5I GGATG 8 cut(s) 676, 770, 1051, 1328, 2117, 2813, 2846, 3068
BstFNI CGCG 1 cut(s) 2350
BstMAI GTCTC 5 cut(s) 170, 534, 720, 1342, 1662
BstNI CCWGG 5 cut(s) 78, 1032, 2044, 2366, 2712
BstNSI RCATGY 3 cut(s) 797, 1531, 2520
BstPI GGTNACC 2 cut(s) 236, 1961
BstSCI CCNGG 7 cut(s) 76, 1030, 2042, 2364, 2548, 2710, 2910
BstSFI CTRYAG 2 cut(s) 2916, 3123
BstSLI GKGCMC 2 cut(s) 1084, 2546
BstUI CGCG 1 cut(s) 2350
BstV1I GCAGC 9 cut(s) 438, 736, 1176, 1238, 2141, 2276, 2497, 2756, 2902
BstV2I GAAGAC 1 cut(s) 775
BstX2I RGATCY 4 cut(s) 14, 329, 1433, 1853
BstYI RGATCY 4 cut(s) 14, 329, 1433, 1853
BstZ17I GTATAC 1 cut(s) 1249
Bsu15I ATCGAT 3 cut(s) 777, 2145, 2292
BsuI GTATCC 1 cut(s) 2096
BsuRI GGCC 6 cut(s) 83, 369, 1192, 1615, 1654, 2136
BsuTUI ATCGAT 3 cut(s) 777, 2145, 2292
BtgI CCRYGG 6 cut(s) 136, 492, 913, 1464, 1660, 1755
BtgZI GCGATG 1 cut(s) 591
BtsCI GGATG 8 cut(s) 676, 770, 1051, 1328, 2117, 2813, 2846, 3068
BtsI GCAGTG 3 cut(s) 1351, 2869, 3120
BtsIMutI CAGTG 8 cut(s) 1351, 1741, 1902, 1938, 2460, 2748, 2869, 3120
BveI ACCTGC 3 cut(s) 224, 484, 1031
Cac8I GCNNGC 7 cut(s) 169, 258, 1100, 1185, 2410, 2518, 2860
CaiI CAGNNNCTG 4 cut(s) 1232, 2567, 2750, 2924
CciI TCATGA 1 cut(s) 442
Cfr10I RCCGGY 5 cut(s) 256, 365, 380, 1958, 2961
ClaI ATCGAT 3 cut(s) 777, 2145, 2292
CseI GACGC 3 cut(s) 309, 1478, 2573
Csp6I GTAC 5 cut(s) 151, 964, 2254, 2479, 2959
CspAI ACCGGT 1 cut(s) 380
CviQI GTAC 5 cut(s) 151, 964, 2254, 2479, 2959
DdeI CTNAG 7 cut(s) 663, 1076, 1733, 2616, 2686, 2719, 2991
DraIII CACNNNGTG 1 cut(s) 57
EaeI YGGCCR 1 cut(s) 1652
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
EciI GGCGGA 1 cut(s) 200
Eco130I CCWWGG 4 cut(s) 913, 1464, 1660, 2537
Eco147I AGGCCT 1 cut(s) 2136
Eco24I GRGCYC 3 cut(s) 906, 1147, 2665
Eco31I GGTCTC 2 cut(s) 170, 1342
Eco32I GATATC 1 cut(s) 1826
Eco57I CTGAAG 2 cut(s) 236, 769
Eco91I GGTNACC 2 cut(s) 236, 1961
EcoO65I GGTNACC 2 cut(s) 236, 1961
EcoRII CCWGG 5 cut(s) 76, 1030, 2042, 2364, 2710
EcoRV GATATC 1 cut(s) 1826
EcoT14I CCWWGG 4 cut(s) 913, 1464, 1660, 2537
EcoT22I ATGCAT 1 cut(s) 3068
EcoT38I GRGCYC 3 cut(s) 906, 1147, 2665
ErhI CCWWGG 4 cut(s) 913, 1464, 1660, 2537
Esp3I CGTCTC 1 cut(s) 534
FalI AAGNNNNNCTT 2 cut(s) 2269, 2301
FaqI GGGAC 3 cut(s) 747, 812, 2626
FauI CCCGC 2 cut(s) 553, 1124
FbaI TGATCA 2 cut(s) 439, 456
FblI GTMKAC 6 cut(s) 61, 1178, 1248, 2039, 2313, 2696
FokI GGATG 8 cut(s) 683, 757, 1058, 1335, 2104, 2800, 2853, 3075
FriOI GRGCYC 3 cut(s) 906, 1147, 2665
FspBI CTAG 9 cut(s) 32, 288, 929, 1103, 1148, 1194, 1638, 1812, 2126
GsaI CCCAGC 3 cut(s) 1191, 1380, 2492
HaeIII GGCC 6 cut(s) 83, 369, 1192, 1615, 1654, 2136
HgaI GACGC 3 cut(s) 309, 1478, 2573
Hin1I GRCGYC 1 cut(s) 1470
HincII GTYRAC 5 cut(s) 504, 525, 1365, 1887, 1924
HindII GTYRAC 5 cut(s) 504, 525, 1365, 1887, 1924
HindIII AAGCTT 2 cut(s) 275, 512
HinfI GANTC 9 cut(s) 617, 841, 890, 944, 1354, 1372, 1844, 1980, 2435
Hpy99I CGWCG 1 cut(s) 430
HpyAV CCTTC 8 cut(s) 28, 1615, 1976, 2126, 2227, 2295, 2403, 3154
HpyCH4IV ACGT 4 cut(s) 527, 602, 818, 2957
HpyF3I CTNAG 7 cut(s) 663, 1076, 1733, 2616, 2686, 2719, 2991
HpySE526I ACGT 4 cut(s) 527, 602, 818, 2957
Hsp92I GRCGYC 1 cut(s) 1470
Kpn2I TCCGGA 1 cut(s) 743
KpnI GGTACC 1 cut(s) 967
KroI GCCGGC 1 cut(s) 256
KroNI GCCGGC 1 cut(s) 258
Ksp22I TGATCA 2 cut(s) 439, 456
Lsp1109I GCAGC 9 cut(s) 438, 736, 1176, 1238, 2141, 2276, 2497, 2756, 2902
LweI GCATC 7 cut(s) 1036, 1062, 1225, 2395, 2580, 2890, 3053
MaeI CTAG 9 cut(s) 32, 288, 929, 1103, 1148, 1194, 1638, 1812, 2126
MaeII ACGT 4 cut(s) 527, 602, 818, 2957
MfeI CAATTG 1 cut(s) 2829
MflI RGATCY 4 cut(s) 14, 329, 1433, 1853
MhlI GDGCHC 5 cut(s) 906, 1084, 1147, 2546, 2665
MlyI GAGTC 3 cut(s) 850, 938, 2444
MmeI TCCRAC 4 cut(s) 577, 648, 1957, 2092
Mph1103I ATGCAT 1 cut(s) 3068
MroI TCCGGA 1 cut(s) 743
MroNI GCCGGC 1 cut(s) 256
MroXI GAANNNNTTC 1 cut(s) 701
MslI CAYNNNNRTG 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 2488
MspR9I CCNGG 7 cut(s) 78, 1032, 2044, 2366, 2550, 2712, 2912
MunI CAATTG 1 cut(s) 2829
Mva1269I GAATGC 3 cut(s) 1797, 2366, 2992
MvaI CCWGG 5 cut(s) 78, 1032, 2044, 2366, 2712
MvnI CGCG 1 cut(s) 2350
NaeI GCCGGC 1 cut(s) 258
NciI CCSGG 2 cut(s) 2550, 2912
NcoI CCATGG 3 cut(s) 913, 1464, 1660
NgoMIV GCCGGC 1 cut(s) 256
NmuCI GTSAC 7 cut(s) 236, 622, 1501, 1763, 1933, 1961, 2071
NsiI ATGCAT 1 cut(s) 3068
NspI RCATGY 3 cut(s) 797, 1531, 2520
NspV TTCGAA 2 cut(s) 203, 2327
OliI CACNNNNGTG 1 cut(s) 407
PaeI GCATGC 1 cut(s) 2520
PagI TCATGA 1 cut(s) 442
PaqCI CACCTGC 2 cut(s) 224, 484
PceI AGGCCT 1 cut(s) 2136
PciI ACATGT 1 cut(s) 793
PctI GAATGC 3 cut(s) 1797, 2366, 2992
PdiI GCCGGC 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 701
PfeI GAWTC 6 cut(s) 617, 890, 1354, 1372, 1844, 1980
PflMI CCANNNNNTGG 1 cut(s) 973
PfoI TCCNGGA 1 cut(s) 1030
PinAI ACCGGT 1 cut(s) 380
PleI GAGTC 3 cut(s) 849, 938, 2443
PpsI GAGTC 3 cut(s) 849, 938, 2443
Ppu21I YACGTR 2 cut(s) 819, 2958
PscI ACATGT 1 cut(s) 793
PshBI ATTAAT 1 cut(s) 2607
PsiI TTATAA 1 cut(s) 557
Psp6I CCWGG 5 cut(s) 76, 1030, 2042, 2364, 2710
PspEI GGTNACC 2 cut(s) 236, 1961
PspFI CCCAGC 3 cut(s) 1187, 1376, 2488
PspGI CCWGG 5 cut(s) 76, 1030, 2042, 2364, 2710
PsrI GAACNNNNNNTAC 4 cut(s) 2622, 2654, 3101, 3133
PstI CTGCAG 2 cut(s) 2920, 3127
PstNI CAGNNNCTG 4 cut(s) 1232, 2567, 2750, 2924
PsuI RGATCY 4 cut(s) 14, 329, 1433, 1853
PvuII CAGCTG 1 cut(s) 2488
RsaI GTAC 5 cut(s) 152, 965, 2255, 2480, 2960
RsaNI GTAC 5 cut(s) 151, 964, 2254, 2479, 2959
RseI CAYNNNNRTG 1 cut(s) 407
SchI GAGTC 3 cut(s) 850, 938, 2444
ScrFI CCNGG 7 cut(s) 78, 1032, 2044, 2366, 2550, 2712, 2912
SduI GDGCHC 5 cut(s) 906, 1084, 1147, 2546, 2665
SfaNI GCATC 7 cut(s) 1036, 1062, 1225, 2395, 2580, 2890, 3053
SfcI CTRYAG 2 cut(s) 2916, 3123
SfuI TTCGAA 2 cut(s) 203, 2327
SgrAI CRCCGGYG 1 cut(s) 1958
SmiMI CAYNNNNRTG 1 cut(s) 407
SmlI CTYRAG 2 cut(s) 1289, 1644
SmoI CTYRAG 2 cut(s) 1289, 1644
SphI GCATGC 1 cut(s) 2520
SseBI AGGCCT 1 cut(s) 2136
SsiI CCGC 7 cut(s) 211, 479, 546, 978, 1131, 2348, 2907
SspMI CTAG 9 cut(s) 32, 288, 929, 1103, 1148, 1194, 1638, 1812, 2126
StuI AGGCCT 1 cut(s) 2136
StyD4I CCNGG 7 cut(s) 76, 1030, 2042, 2364, 2548, 2710, 2910
StyI CCWWGG 4 cut(s) 913, 1464, 1660, 2537
TaiI ACGT 4 cut(s) 530, 605, 821, 2960
TaqI TCGA 7 cut(s) 203, 777, 1019, 1298, 2145, 2292, 2327
TaqII GACCGA 1 cut(s) 2685
TatI WGTACW 1 cut(s) 150
TauI GCSGC 1 cut(s) 981
TfiI GAWTC 6 cut(s) 617, 890, 1354, 1372, 1844, 1980
TscAI CASTG 8 cut(s) 1351, 1741, 1909, 1938, 2467, 2755, 2869, 3127
TseFI GTSAC 7 cut(s) 236, 622, 1501, 1763, 1933, 1961, 2071
Tsp45I GTSAC 7 cut(s) 236, 622, 1501, 1763, 1933, 1961, 2071
TspDTI ATGAA 4 cut(s) 694, 1900, 2209, 2999
TspGWI ACGGA 3 cut(s) 1712, 1744, 2714
TspRI CASTG 8 cut(s) 1351, 1741, 1909, 1938, 2467, 2755, 2869, 3127
Van91I CCANNNNNTGG 1 cut(s) 973
VneI GTGCAC 1 cut(s) 1080
VspI ATTAAT 1 cut(s) 2607
XapI RAATTY 4 cut(s) 349, 1215, 1279, 1748
XbaI TCTAGA 2 cut(s) 1147, 2125
XceI RCATGY 3 cut(s) 797, 1531, 2520
XmiI GTMKAC 6 cut(s) 61, 1178, 1248, 2039, 2313, 2696
XmnI GAANNNNTTC 1 cut(s) 701
XspI CTAG 9 cut(s) 32, 288, 929, 1103, 1148, 1194, 1638, 1812, 2126
Zsp2I ATGCAT 1 cut(s) 3068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.