AT1G14350
MYB Family

ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity. Represses the expression of the mitosis-inducing factors CDKB1-1 and CDKA-1, specifically required for the last guard mother cells (GMC) symmetric divisions in the stomatal pathway. Represses CYCA2-3 in newly formed guard cells. Together with MYB88, regulates stomata spacing by restricting divisions late in the stomatal cell lineage thus limiting the number of GMC divisions. In collaboration with CDKB1-1 and CDKB1-2, restrict the G1 S transition and chloroplast and nuclear number during stomatal formation, and normally maintain fate and developmental progression throughout the stomatal cell lineage

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002009
Physical Location & Seq
Forward (+)
4907887 .. 4911168
3282 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G14350.3

Sequence Viewer

Length: 1311 bp
ATGGAAGATACGAAGAAGAAAAAGAAGAAGAATATTAACAACAACCAAGATTCCAAGAAAAAAGAGCGTCATATTGTTACTTGGTCACAAGAGGAAGATGTTATACTAAGAGAACAAATAACTCTACATGGAACTGAAAATTGGGCGATCATTGCATCAAAGTTCAAAGACAAAAGCACAAGACAATGCAGAAGAAGATGGTATACATACTTAAACTCTGATTTCAAAAGAGGAGGTTGGTCCCCTGAAGAAGATATGCTTTTGTGTGAGGCACAAAGAGTATTTGGTAATAGATGGACTGAGATAGCAAAGGTGGTTTCAGGCAGAACTGATAATGCTGTGAAGAACAGGTTTACAACACTTTGTAAGAAGAGAGCCAAGCATGAAGCTATGACTAAAGACAGCAACTCAAACACCAAAAGAATGTTGTTCTTAGACGGTATCAGTACACCGCGAAAATCCGAGAATGAAACTCCTATTGCTAAGAAATTGAAGAGAAGTCACATTCTAGATCTCACAGAGATCAGTAACTATGGAAGGGCCGAGGCATGTGTGAATCAGCAGATAAGGTCTCCATTTTCGGTTTTAGCTCGAAATGCCACTGGTATTGATAGCTTGGAAGAACAGAATCAAACAAGCAATGTGAATGAGAGTGATGGTGAAGGGATGTTTCTTAAGAAGGATGATCCAAAAGTGACAGCTTTGATGCAACAAGCTGAACTTTTAAGCTCCTTGGCGCAGAAAGTTAATGCAGACAACACAGAACAGAGTATGGAGAATGCTTGGAAGGTTCTTCAGGACTTCTTGAATAAAGGCAAGGAGAATGATTTATTCAGATATGGAATACCTGATATAGATTTTAAGATCGAGGAATTTAAGGACCTTATAGAGGATTTGAGGAGTGGTTATGAAGACAATCAATTATCTTGGAGGCAACCTGATCTTCATGACTCACCAGCTAGCTCTGAGTATAGTTCCGGATCAACCATCATGGTGGATCAGTCTGGTGATAAAACACAACCATTCTCAGCAGATACTCAGACAGAACATAAGCAAGTTGGAGAGGAGTTGCTCGTCCCGAAAAATCCTGATGAGAATATGCCCATCTCAGGCGAAGAAAAGTTCAGCTCGCCTATTCAGGTCACGCCATTGTTCAGATCTTTGGCAGACGGTATACCAAGTCCACAGTTCTCCGAAAGTGAGAGGAGCTTCCTGCTAAAAACACTAGGGATCGAGTCCTCATCTCCATGTCCAAGTGCTAATCCTTCAAAACCACCCCCTTGCAAAAGAGTCCTTCTCCATAGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006275 GO:0006355 GO:0006357 GO:0006810 GO:0007049 GO:0007275 GO:0008150 GO:0009553 GO:0009554 GO:0009628 GO:0009629 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009737 GO:0009791 GO:0009888 GO:0009889 GO:0009893 GO:0009914 GO:0009926 GO:0009987 GO:0010016 GO:0010033 GO:0010052 GO:0010103 GO:0010235 GO:0010374 GO:0010376 GO:0010440 GO:0010444 GO:0010468 GO:0010556 GO:0010564 GO:0010604 GO:0010638 GO:0010817 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022622 GO:0030104 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032506 GO:0032875 GO:0033043 GO:0033044 GO:0033993 GO:0034293 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043934 GO:0044212 GO:0044424 GO:0044464 GO:0044703 GO:0047484 GO:0048229 GO:0048236 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0048871 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050878 GO:0050891 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0060255 GO:0060918 GO:0061085 GO:0061087 GO:0061640 GO:0065007 GO:0065008 GO:0080022 GO:0080090 GO:0080134 GO:0090329 GO:0090436 GO:0090558 GO:0090626 GO:0090627 GO:0090696 GO:0090698 GO:0097159 GO:0097305 GO:0099402 GO:0140110 GO:1901000 GO:1901002 GO:1901333 GO:1901363 GO:1901700 GO:1901987 GO:1902275 GO:1902410 GO:1902584 GO:1902806 GO:1903046 GO:1903047 GO:1903506 GO:1905269 GO:2000023 GO:2000026 GO:2000037 GO:2000069 GO:2000070 GO:2000112 GO:2000280 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

436

Amino Acids

49.6

Weight (kDa)

6.45

Isoelectric Point (pI)

57.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 34 - 77 2.3e-13 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 37 - 86 1.3e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 203, 1174
AccII CGCG 1 cut(s) 454
AccIII TCCGGA 1 cut(s) 977
AciI CCGC 1 cut(s) 452
AclWI GGATC 4 cut(s) 680, 988, 1005, 1238
AcsI RAATTY 1 cut(s) 872
AcuI CTGAAG 2 cut(s) 267, 779
AfaI GTAC 1 cut(s) 448
AfiI CCNNNNNNNGG 2 cut(s) 889, 1109
AflII CTTAAG 1 cut(s) 674
AgsI TTSAA 5 cut(s) 166, 226, 493, 808, 1269
AjuI GAANNNNNNNTTGG 2 cut(s) 124, 156
Alw26I GTCTC 1 cut(s) 576
AlwI GGATC 4 cut(s) 680, 988, 1005, 1238
Aor13HI TCCGGA 1 cut(s) 977
AoxI GGCC 1 cut(s) 540
ApoI RAATTY 1 cut(s) 872
AspLEI GCGC 1 cut(s) 739
AspS9I GGNCC 3 cut(s) 240, 540, 880
AsuHPI GGTGA 3 cut(s) 671, 945, 1019
AsuNHI GCTAGC 1 cut(s) 959
AvaII GGWCC 2 cut(s) 240, 880
BarI GAAGNNNNNNTAC 4 cut(s) 87, 119, 187, 219
BbsI GAAGAC 1 cut(s) 918
BccI CCATC 5 cut(s) 192, 288, 650, 995, 1112
BcoDI GTCTC 1 cut(s) 576
BfaI CTAG 3 cut(s) 509, 960, 1226
BfrI CTTAAG 1 cut(s) 674
BglII AGATCT 2 cut(s) 511, 1157
Bme18I GGWCC 2 cut(s) 240, 880
BmgT120I GGNCC 3 cut(s) 240, 540, 880
BmiI GGNNCC 1 cut(s) 242
BmsI GCATC 2 cut(s) 164, 696
BmtI GCTAGC 1 cut(s) 963
BpiI GAAGAC 1 cut(s) 918
BplI GAGNNNNNCTC 1 cut(s) 1281
BsaI GGTCTC 1 cut(s) 576
BsaJI CCNNGG 2 cut(s) 543, 732
BsaWI WCCGGW 1 cut(s) 977
Bsc4I CCNNNNNNNGG 2 cut(s) 889, 1109
Bse1I ACTGG 1 cut(s) 607
Bse3DI GCAATG 2 cut(s) 150, 646
BseAI TCCGGA 1 cut(s) 977
BseDI CCNNGG 2 cut(s) 543, 732
BseGI GGATG 2 cut(s) 672, 688
BseLI CCNNNNNNNGG 2 cut(s) 889, 1109
BseMI GCAATG 2 cut(s) 150, 646
BseMII CTCAG 5 cut(s) 291, 957, 1041, 1052, 1122
BseNI ACTGG 1 cut(s) 607
BseRI GAGGAG 4 cut(s) 246, 913, 1079, 1219
Bsh1236I CGCG 1 cut(s) 454
BshFI GGCC 1 cut(s) 542
BsiSI CCGG 1 cut(s) 978
BslFI GGGAC 2 cut(s) 226, 1061
BslI CCNNNNNNNGG 2 cut(s) 889, 1109
BsmAI GTCTC 1 cut(s) 576
BsmFI GGGAC 2 cut(s) 226, 1061
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 1 cut(s) 542
Bso31I GGTCTC 1 cut(s) 576
Bsp13I TCCGGA 1 cut(s) 977
BspACI CCGC 1 cut(s) 452
BspANI GGCC 1 cut(s) 542
BspCNI CTCAG 5 cut(s) 292, 958, 1040, 1051, 1121
BspEI TCCGGA 1 cut(s) 977
BspFNI CGCG 1 cut(s) 454
BspHI TCATGA 1 cut(s) 946
BspLI GGNNCC 1 cut(s) 242
BspOI GCTAGC 1 cut(s) 963
BspPI GGATC 4 cut(s) 680, 988, 1005, 1238
BspTI CTTAAG 1 cut(s) 674
BspTNI GGTCTC 1 cut(s) 576
BsrDI GCAATG 2 cut(s) 150, 646
BsrI ACTGG 1 cut(s) 607
BssECI CCNNGG 2 cut(s) 543, 732
BssNAI GTATAC 2 cut(s) 204, 1175
BssT1I CCWWGG 1 cut(s) 732
Bst1107I GTATAC 2 cut(s) 204, 1175
Bst4CI ACNGT 3 cut(s) 440, 1172, 1188
Bst6I CTCTTC 2 cut(s) 365, 488
BstAFI CTTAAG 1 cut(s) 674
BstC8I GCNNGC 2 cut(s) 961, 1130
BstDEI CTNAG 8 cut(s) 107, 300, 433, 483, 966, 1027, 1038, 1108
BstENI CCTNNNNNAGG 1 cut(s) 887
BstF5I GGATG 2 cut(s) 672, 688
BstFNI CGCG 1 cut(s) 454
BstHHI GCGC 1 cut(s) 739
BstMAI GTCTC 1 cut(s) 576
BstMWI GCNNNNNNNGC 2 cut(s) 152, 596
BstNSI RCATGY 1 cut(s) 552
BstUI CGCG 1 cut(s) 454
BstV2I GAAGAC 1 cut(s) 918
BstX2I RGATCY 2 cut(s) 511, 1157
BstXI CCANNNNNNTGG 1 cut(s) 994
BstYI RGATCY 2 cut(s) 511, 1157
BstZ17I GTATAC 2 cut(s) 204, 1175
BsuRI GGCC 1 cut(s) 542
BtsCI GGATG 2 cut(s) 672, 688
BtsIMutI CAGTG 1 cut(s) 600
Cac8I GCNNGC 2 cut(s) 961, 1130
CciI TCATGA 1 cut(s) 946
CfoI GCGC 1 cut(s) 739
Cfr13I GGNCC 3 cut(s) 240, 540, 880
CseI GACGC 1 cut(s) 56
Csp6I GTAC 1 cut(s) 447
CspCI CAANNNNNGTGG 2 cut(s) 589, 624
CviAII CATG 6 cut(s) 128, 383, 549, 947, 991, 1248
CviQI GTAC 1 cut(s) 447
DdeI CTNAG 8 cut(s) 107, 300, 433, 483, 966, 1027, 1038, 1108
Eam1104I CTCTTC 2 cut(s) 365, 488
EarI CTCTTC 2 cut(s) 365, 488
Eco130I CCWWGG 1 cut(s) 732
Eco31I GGTCTC 1 cut(s) 576
Eco47I GGWCC 2 cut(s) 240, 880
Eco57I CTGAAG 2 cut(s) 267, 779
EcoNI CCTNNNNNAGG 1 cut(s) 887
EcoO109I RGGNCCY 1 cut(s) 880
EcoT14I CCWWGG 1 cut(s) 732
ErhI CCWWGG 1 cut(s) 732
FaeI CATG 6 cut(s) 131, 386, 552, 950, 994, 1251
FalI AAGNNNNNCTT 4 cut(s) 243, 275, 705, 737
FaqI GGGAC 2 cut(s) 226, 1061
FatI CATG 6 cut(s) 127, 382, 548, 946, 990, 1247
FblI GTMKAC 2 cut(s) 203, 1174
FokI GGATG 2 cut(s) 679, 695
FspBI CTAG 3 cut(s) 509, 960, 1226
GlaI GCGC 1 cut(s) 738
HaeIII GGCC 1 cut(s) 542
HapII CCGG 1 cut(s) 978
HgaI GACGC 1 cut(s) 56
HhaI GCGC 1 cut(s) 739
Hin1II CATG 6 cut(s) 131, 386, 552, 950, 994, 1251
Hin6I GCGC 1 cut(s) 737
HinP1I GCGC 1 cut(s) 737
HinfI GANTC 6 cut(s) 50, 556, 628, 950, 1235, 1290
HpaII CCGG 1 cut(s) 978
HphI GGTGA 3 cut(s) 671, 945, 1019
Hpy166II GTNNAC 5 cut(s) 204, 354, 449, 1175, 1184
Hpy188I TCNGA 7 cut(s) 220, 463, 836, 967, 1041, 1157, 1195
Hpy188III TCNNGA 7 cut(s) 509, 797, 805, 947, 978, 1078, 1088
Hpy8I GTNNAC 5 cut(s) 204, 354, 449, 1175, 1184
HpyAV CCTTC 6 cut(s) 531, 656, 673, 781, 1275, 1304
HpyCH4III ACNGT 3 cut(s) 440, 1172, 1188
HpyCH4V TGCA 5 cut(s) 155, 189, 709, 752, 1284
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 596
HpyF3I CTNAG 8 cut(s) 107, 300, 433, 483, 966, 1027, 1038, 1108
Hsp92II CATG 6 cut(s) 131, 386, 552, 950, 994, 1251
HspAI GCGC 1 cut(s) 737
Kpn2I TCCGGA 1 cut(s) 977
LmnI GCTCC 2 cut(s) 734, 1206
LweI GCATC 2 cut(s) 164, 696
MaeI CTAG 3 cut(s) 509, 960, 1226
MaeIII GTNAC 6 cut(s) 76, 84, 500, 527, 694, 1141
MflI RGATCY 2 cut(s) 511, 1157
MluCI AATT 4 cut(s) 139, 488, 872, 920
MlyI GAGTC 3 cut(s) 944, 1244, 1299
MmeI TCCRAC 1 cut(s) 1039
MroI TCCGGA 1 cut(s) 977
MseI TTAA 7 cut(s) 36, 212, 675, 725, 747, 861, 876
MslI CAYNNNNRTG 2 cut(s) 992, 1246
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 1 cut(s) 978
Mva1269I GAATGC 1 cut(s) 784
MvnI CGCG 1 cut(s) 454
MwoI GCNNNNNNNGC 2 cut(s) 152, 596
NheI GCTAGC 1 cut(s) 959
NlaIII CATG 6 cut(s) 131, 386, 552, 950, 994, 1251
NlaIV GGNNCC 1 cut(s) 242
NmeAIII GCCGAG 1 cut(s) 568
NmuCI GTSAC 4 cut(s) 84, 500, 694, 1141
NspI RCATGY 1 cut(s) 552
PagI TCATGA 1 cut(s) 946
PctI GAATGC 1 cut(s) 784
PfeI GAWTC 3 cut(s) 50, 556, 628
PleI GAGTC 3 cut(s) 944, 1243, 1298
PpsI GAGTC 3 cut(s) 944, 1243, 1298
PpuMI RGGWCCY 1 cut(s) 880
Psp5II RGGWCCY 1 cut(s) 880
PspN4I GGNNCC 1 cut(s) 242
PspPI GGNCC 3 cut(s) 240, 540, 880
PspPPI RGGWCCY 1 cut(s) 880
PsuI RGATCY 2 cut(s) 511, 1157
RsaI GTAC 1 cut(s) 448
RsaNI GTAC 1 cut(s) 447
RseI CAYNNNNRTG 2 cut(s) 992, 1246
SaqAI TTAA 7 cut(s) 36, 212, 675, 725, 747, 861, 876
Sau96I GGNCC 3 cut(s) 240, 540, 880
SchI GAGTC 3 cut(s) 944, 1244, 1299
SfaNI GCATC 2 cut(s) 164, 696
SinI GGWCC 2 cut(s) 240, 880
SmiMI CAYNNNNRTG 2 cut(s) 992, 1246
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
Sse9I AATT 4 cut(s) 139, 488, 872, 920
SsiI CCGC 1 cut(s) 452
SspI AATATT 1 cut(s) 34
SspMI CTAG 3 cut(s) 509, 960, 1226
StyI CCWWGG 1 cut(s) 732
TaaI ACNGT 3 cut(s) 440, 1172, 1188
TaqI TCGA 3 cut(s) 592, 867, 1233
TasI AATT 4 cut(s) 139, 488, 872, 920
TatI WGTACW 1 cut(s) 446
TfiI GAWTC 3 cut(s) 50, 556, 628
Tru1I TTAA 7 cut(s) 36, 212, 675, 725, 747, 861, 876
Tru9I TTAA 7 cut(s) 36, 212, 675, 725, 747, 861, 876
TscAI CASTG 1 cut(s) 607
TseFI GTSAC 4 cut(s) 84, 500, 694, 1141
Tsp45I GTSAC 4 cut(s) 84, 500, 694, 1141
TspDTI ATGAA 4 cut(s) 399, 483, 924, 935
TspRI CASTG 1 cut(s) 607
Vha464I CTTAAG 1 cut(s) 674
VpaK11BI GGWCC 2 cut(s) 240, 880
XagI CCTNNNNNAGG 1 cut(s) 887
XapI RAATTY 1 cut(s) 872
XbaI TCTAGA 1 cut(s) 508
XceI RCATGY 1 cut(s) 552
XmiI GTMKAC 2 cut(s) 203, 1174
XspI CTAG 3 cut(s) 509, 960, 1226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.