MD13G1075000.v1.1
MYB Family

SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002009
Physical Location & Seq
Forward (+)
5304330 .. 5309000
4671 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1075000.v1.1.491

Sequence Viewer

Length: 1410 bp
ATGCCGCAGGAGGAGTCAAAGAAGAAGGAGCGCCACATCGTTAGTTGGTCTCAAGAGGAAGATGATATACTGAGGAATCAAATTAACACCCATGGAACAGAAAATTGGGCTATCATTGCATCTAAATTCAAGGATAAAACAACCAGACAGTGCAGGAGAAGATGGTACACATACTTGAATTCTGACTTCAAGAAAGGAGGGTGGTCACCAGAGGAAGACATGCTCTTGTGCGAGGCTCAAAAGATATTCGGAAACAGATGGACAGAAATAGCGAAGGTTGTTTCGGGAAGAACTGACAATGCTGTGAAAAACCGGTTTTCCACCTTGTGCAAGAAGAGAGCAAAATATGAAGCCTTAGCTAAAGAGAATGCCGCTTCATATATCAATCAGAATGACAAAAGGGTCAAAATCCGAAATGGATTCAATACAGATGGAACAGCAGAAACTACAGCACCTTCTAAGAAGATGAGGAGAAGCCACATCCCTAGTCTCTCCGAGGGGGACAGACTACTCGAGCAGTGTGGAAAGATGAGTCAGCAGCTAAGAGCTCCATTTGCAGTGTTGATTCAGAATGTCCACAATGTAGAGAATCTGCCAGACCAGAATAATGTGAATGGCACCAAGGAAGTCCCCGTGAATGCAGTACAAAAGAGCAAGTTTCACGGATCATTTCTCAAGAAGGATGATCCGAAGATAATCGCTTTGATGCAACAAGCAGAATTGCTCAGCTCGCTGGCATTAAAAGTTAATGCAGAGAACACAGACCAGAGTCTTGAAAATGCATGGAAGGTGCTCCAAGATTTCTTGAATCAAAGCAAAGATAGTGACATCCTCAGTTATGGAATTAACGATTTTGATTTTCAACTCGAAGATCTTAAATATCTGTTAGAGGACTTAAGGAGCACCACTGAAGGAAGCCGACCATCTTGGCAACATTGCAGGCAGCCTGATTTATACGAGGAGTCTCCAGGAAGTTCCGAATACAGTACAGGGTCAACTCTTCTGTCCCAAACAGAATACTATCAAGTGGAAAAAAATGAAGTTGAAATAGGTTCACTGAATCAGGAGATTCGACCAGGGTCACAGTCAATTCCTATTGAAGGGAAAAATGGTGTTGGTGATTGTGAGAAAGGGATTTTTTCCGAAAAGCAAGAGATATTTCCATCATGTGATGAAGCAACAAAAGACTACGCAGTTGTTTCTGCATTGTCAAGTATAGAGTTCAATTCTCCTATTAAAGTTACCCCATTGTTCAGATCCTTGGCAGCAGGAATTCCTAGCCCAAAATTTTCTGAAAGTGAAAGGAATTTCTTGCTCAAAACGCTTGGAGAGGACTCCCCCTGCCCCAACCCAAGCACCAATCCTTCACAACCGCCACCCTGCAAACGATCCCTTCTCCAAAGTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006275 GO:0006355 GO:0006357 GO:0006810 GO:0007049 GO:0007275 GO:0008150 GO:0009553 GO:0009554 GO:0009628 GO:0009629 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009737 GO:0009791 GO:0009888 GO:0009889 GO:0009893 GO:0009914 GO:0009926 GO:0009987 GO:0010016 GO:0010033 GO:0010052 GO:0010103 GO:0010235 GO:0010374 GO:0010376 GO:0010440 GO:0010444 GO:0010468 GO:0010556 GO:0010564 GO:0010604 GO:0010638 GO:0010817 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022622 GO:0030104 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032506 GO:0032875 GO:0033043 GO:0033044 GO:0033993 GO:0034293 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043934 GO:0044212 GO:0044424 GO:0044464 GO:0044703 GO:0047484 GO:0048229 GO:0048236 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0048871 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050878 GO:0050891 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0060255 GO:0060918 GO:0061085 GO:0061087 GO:0061640 GO:0065007 GO:0065008 GO:0080022 GO:0080090 GO:0080134 GO:0090329 GO:0090436 GO:0090558 GO:0090626 GO:0090627 GO:0090696 GO:0090698 GO:0097159 GO:0097305 GO:0099402 GO:0140110 GO:1901000 GO:1901002 GO:1901333 GO:1901363 GO:1901700 GO:1901987 GO:1902275 GO:1902410 GO:1902584 GO:1902806 GO:1903046 GO:1903047 GO:1903506 GO:1905269 GO:2000023 GO:2000026 GO:2000037 GO:2000069 GO:2000070 GO:2000112 GO:2000280 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

470

Amino Acids

52.99

Weight (kDa)

6.2

Isoelectric Point (pI)

61.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 15 - 59 5e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 16 - 76 6.5e-15 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 65 - 108 2e-13 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 68 - 115 2.1e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 401
AccB1I GGYRCC 1 cut(s) 617
AciI CCGC 3 cut(s) 5, 372, 1373
AclWI GGATC 4 cut(s) 673, 680, 1251, 1383
AcsI RAATTY 5 cut(s) 125, 178, 1272, 1286, 1306
AcuI CTGAAG 1 cut(s) 930
AdeI CACNNNGTG 1 cut(s) 327
AfaI GTAC 3 cut(s) 167, 645, 988
AfiI CCNNNNNNNGG 1 cut(s) 1100
AflII CTTAAG 1 cut(s) 895
AgeI ACCGGT 1 cut(s) 312
AjnI CCWGG 2 cut(s) 967, 1075
AjuI GAANNNNNNNTTGG 2 cut(s) 88, 120
AluBI AGCT 4 cut(s) 359, 541, 548, 729
AluI AGCT 4 cut(s) 359, 541, 548, 729
Alw21I GWGCWC 3 cut(s) 550, 795, 905
Alw26I GTCTC 3 cut(s) 54, 494, 969
AlwI GGATC 4 cut(s) 673, 680, 1251, 1383
Ama87I CYCGRG 1 cut(s) 512
ApeKI GCWGC 3 cut(s) 538, 943, 1265
ApoI RAATTY 5 cut(s) 125, 178, 1272, 1286, 1306
AsiGI ACCGGT 1 cut(s) 312
AspLEI GCGC 1 cut(s) 33
AsuHPI GGTGA 2 cut(s) 198, 1130
AvaI CYCGRG 1 cut(s) 512
BanI GGYRCC 1 cut(s) 617
BanII GRGCYC 1 cut(s) 550
BarI GAAGNNNNNNTAC 2 cut(s) 51, 83
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 3 cut(s) 550, 795, 905
BbvI GCAGC 3 cut(s) 550, 955, 1277
BccI CCATC 5 cut(s) 156, 252, 425, 931, 1171
BciT130I CCWGG 2 cut(s) 969, 1077
BcoDI GTCTC 3 cut(s) 54, 494, 969
BfaI CTAG 2 cut(s) 486, 1278
BfmI CTRYAG 1 cut(s) 447
BfoI RGCGCY 1 cut(s) 34
BfrI CTTAAG 1 cut(s) 895
BglII AGATCT 1 cut(s) 871
BisI GCNGC 5 cut(s) 5, 372, 539, 944, 1266
BlpI GCTNAGC 1 cut(s) 725
BlsI GCNGC 5 cut(s) 6, 373, 540, 945, 1267
Bme1390I CCNGG 2 cut(s) 969, 1077
BmeT110I CYCGRG 1 cut(s) 512
BmiI GGNNCC 1 cut(s) 619
BmrFI CCNGG 2 cut(s) 969, 1077
BmsI GCATC 2 cut(s) 128, 696
BoxI GACNNNNGTC 2 cut(s) 768, 1078
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 951
Bpu10I CCTNAGC 1 cut(s) 355
Bpu1102I GCTNAGC 1 cut(s) 725
BpuEI CTTGAG 2 cut(s) 36, 659
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 5 cut(s) 91, 495, 621, 1076, 1260
BsaWI WCCGGW 1 cut(s) 312
Bsc4I CCNNNNNNNGG 1 cut(s) 1100
Bse118I RCCGGY 1 cut(s) 312
Bse3DI GCAATG 2 cut(s) 114, 934
BseBI CCWGG 2 cut(s) 969, 1077
BseDI CCNNGG 5 cut(s) 91, 495, 621, 1076, 1260
BseGI GGATG 3 cut(s) 480, 688, 828
BseLI CCNNNNNNNGG 1 cut(s) 1100
BseMI GCAATG 2 cut(s) 114, 934
BseMII CTCAG 3 cut(s) 62, 739, 847
BseRI GAGGAG 3 cut(s) 26, 484, 974
BseXI GCAGC 3 cut(s) 550, 955, 1277
BsgI GTGCAG 1 cut(s) 172
BshNI GGYRCC 1 cut(s) 617
BshTI ACCGGT 1 cut(s) 312
BsiHKAI GWGCWC 3 cut(s) 550, 795, 905
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 1 cut(s) 313
BslFI GGGAC 3 cut(s) 515, 614, 991
BslI CCNNNNNNNGG 1 cut(s) 1100
BsmAI GTCTC 3 cut(s) 54, 494, 969
BsmFI GGGAC 3 cut(s) 515, 614, 991
BsmI GAATGC 2 cut(s) 373, 643
Bso31I GGTCTC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 512
Bsp1286I GDGCHC 3 cut(s) 550, 795, 905
Bsp143I GATC 5 cut(s) 665, 685, 871, 1256, 1388
Bsp1720I GCTNAGC 1 cut(s) 725
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 3 cut(s) 5, 372, 1373
BspCNI CTCAG 3 cut(s) 63, 738, 846
BspLI GGNNCC 1 cut(s) 619
BspPI GGATC 4 cut(s) 673, 680, 1251, 1383
BspT107I GGYRCC 1 cut(s) 617
BspTI CTTAAG 1 cut(s) 895
BspTNI GGTCTC 1 cut(s) 54
BsrDI GCAATG 2 cut(s) 114, 934
BsrFI RCCGGY 1 cut(s) 312
BssAI RCCGGY 1 cut(s) 312
BssECI CCNNGG 5 cut(s) 91, 495, 621, 1076, 1260
BssMI GATC 5 cut(s) 665, 685, 871, 1256, 1388
BssT1I CCWWGG 3 cut(s) 91, 621, 1260
Bst2UI CCWGG 2 cut(s) 969, 1077
Bst4CI ACNGT 3 cut(s) 150, 986, 1086
Bst6I CTCTTC 2 cut(s) 329, 1005
BstAFI CTTAAG 1 cut(s) 895
BstC8I GCNNGC 3 cut(s) 731, 735, 941
BstDEI CTNAG 6 cut(s) 71, 355, 459, 542, 725, 833
BstDSI CCRYGG 1 cut(s) 91
BstEII GGTNACC 1 cut(s) 204
BstENI CCTNNNNNAGG 1 cut(s) 1098
BstF5I GGATG 3 cut(s) 480, 688, 828
BstH2I RGCGCY 1 cut(s) 34
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 5 cut(s) 668, 688, 874, 1259, 1391
BstMAI GTCTC 3 cut(s) 54, 494, 969
BstMBI GATC 5 cut(s) 665, 685, 871, 1256, 1388
BstMWI GCNNNNNNNGC 4 cut(s) 116, 554, 730, 1321
BstNI CCWGG 2 cut(s) 969, 1077
BstNSI RCATGY 1 cut(s) 223
BstPAI GACNNNNGTC 2 cut(s) 768, 1078
BstPI GGTNACC 1 cut(s) 204
BstSCI CCNGG 2 cut(s) 967, 1075
BstSFI CTRYAG 1 cut(s) 447
BstV1I GCAGC 3 cut(s) 550, 955, 1277
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 2 cut(s) 871, 1256
BstYI RGATCY 2 cut(s) 871, 1256
BtgI CCRYGG 1 cut(s) 91
BtsCI GGATG 3 cut(s) 480, 688, 828
BtsI GCAGTG 2 cut(s) 524, 564
BtsIMutI CAGTG 5 cut(s) 155, 524, 564, 906, 1055
Cac8I GCNNGC 3 cut(s) 731, 735, 941
CfoI GCGC 1 cut(s) 33
Cfr10I RCCGGY 1 cut(s) 312
Csp6I GTAC 3 cut(s) 166, 644, 987
CspAI ACCGGT 1 cut(s) 312
CviAII CATG 4 cut(s) 92, 220, 783, 1167
CviQI GTAC 3 cut(s) 166, 644, 987
DdeI CTNAG 6 cut(s) 71, 355, 459, 542, 725, 833
DpnI GATC 5 cut(s) 667, 687, 873, 1258, 1390
DpnII GATC 5 cut(s) 665, 685, 871, 1256, 1388
DraIII CACNNNGTG 1 cut(s) 327
DrdI GACNNNNNNGTC 1 cut(s) 401
DseDI GACNNNNNNGTC 1 cut(s) 401
Eam1104I CTCTTC 2 cut(s) 329, 1005
EarI CTCTTC 2 cut(s) 329, 1005
Ecl136II GAGCTC 1 cut(s) 548
Eco130I CCWWGG 3 cut(s) 91, 621, 1260
Eco24I GRGCYC 1 cut(s) 550
Eco31I GGTCTC 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 548
Eco57I CTGAAG 1 cut(s) 930
Eco88I CYCGRG 1 cut(s) 512
Eco91I GGTNACC 1 cut(s) 204
EcoICRI GAGCTC 1 cut(s) 548
EcoNI CCTNNNNNAGG 1 cut(s) 1098
EcoO65I GGTNACC 1 cut(s) 204
EcoRI GAATTC 2 cut(s) 178, 1272
EcoRII CCWGG 2 cut(s) 967, 1075
EcoT14I CCWWGG 3 cut(s) 91, 621, 1260
EcoT22I ATGCAT 1 cut(s) 784
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 3 cut(s) 91, 621, 1260
FaeI CATG 4 cut(s) 95, 223, 786, 1170
FaqI GGGAC 3 cut(s) 515, 614, 991
FatI CATG 4 cut(s) 91, 219, 782, 1166
Fnu4HI GCNGC 5 cut(s) 5, 372, 539, 944, 1266
FokI GGATG 3 cut(s) 467, 695, 815
FriOI GRGCYC 1 cut(s) 550
Fsp4HI GCNGC 5 cut(s) 5, 372, 539, 944, 1266
FspBI CTAG 2 cut(s) 486, 1278
GlaI GCGC 1 cut(s) 32
GluI GCNGC 5 cut(s) 5, 372, 539, 944, 1266
GsuI CTGGAG 1 cut(s) 951
HaeII RGCGCY 1 cut(s) 34
HapII CCGG 1 cut(s) 313
HhaI GCGC 1 cut(s) 33
Hin1II CATG 4 cut(s) 95, 223, 786, 1170
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 1 cut(s) 996
HindII GTYRAC 1 cut(s) 996
HpaII CCGG 1 cut(s) 313
HphI GGTGA 2 cut(s) 198, 1130
Hpy166II GTNNAC 4 cut(s) 168, 577, 996, 1055
Hpy188III TCNNGA 7 cut(s) 53, 190, 285, 676, 773, 805, 1064
Hpy8I GTNNAC 4 cut(s) 168, 577, 996, 1055
HpyAV CCTTC 9 cut(s) 19, 268, 465, 673, 781, 905, 1094, 1374, 1403
HpyCH4III ACNGT 3 cut(s) 150, 986, 1086
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 554, 730, 1321
HpyF3I CTNAG 6 cut(s) 71, 355, 459, 542, 725, 833
Hsp92II CATG 4 cut(s) 95, 223, 786, 1170
HspAI GCGC 1 cut(s) 31
Kzo9I GATC 5 cut(s) 665, 685, 871, 1256, 1388
LmnI GCTCC 4 cut(s) 28, 553, 798, 900
Lsp1109I GCAGC 3 cut(s) 550, 955, 1277
LweI GCATC 2 cut(s) 128, 696
MaeI CTAG 2 cut(s) 486, 1278
MaeIII GTNAC 4 cut(s) 204, 824, 1080, 1240
MalI GATC 5 cut(s) 667, 687, 873, 1258, 1390
MboI GATC 5 cut(s) 665, 685, 871, 1256, 1388
MflI RGATCY 2 cut(s) 871, 1256
MhlI GDGCHC 3 cut(s) 550, 795, 905
MlyI GAGTC 5 cut(s) 23, 541, 778, 971, 1328
Mph1103I ATGCAT 1 cut(s) 784
MseI TTAA 7 cut(s) 84, 740, 747, 846, 876, 896, 1236
MspCI CTTAAG 1 cut(s) 895
MspI CCGG 1 cut(s) 313
MspR9I CCNGG 2 cut(s) 969, 1077
Mva1269I GAATGC 2 cut(s) 373, 643
MvaI CCWGG 2 cut(s) 969, 1077
MwoI GCNNNNNNNGC 4 cut(s) 116, 554, 730, 1321
NcoI CCATGG 1 cut(s) 91
NdeII GATC 5 cut(s) 665, 685, 871, 1256, 1388
NlaIII CATG 4 cut(s) 95, 223, 786, 1170
NlaIV GGNNCC 1 cut(s) 619
NmuCI GTSAC 3 cut(s) 204, 824, 1080
NsiI ATGCAT 1 cut(s) 784
NspI RCATGY 1 cut(s) 223
PaeR7I CTCGAG 1 cut(s) 512
PctI GAATGC 2 cut(s) 373, 643
PfeI GAWTC 7 cut(s) 76, 420, 565, 589, 808, 1060, 1069
PfoI TCCNGGA 1 cut(s) 967
PinAI ACCGGT 1 cut(s) 312
PkrI GCNGC 5 cut(s) 6, 373, 540, 945, 1267
PleI GAGTC 5 cut(s) 22, 540, 777, 970, 1328
PpsI GAGTC 5 cut(s) 22, 540, 777, 970, 1328
PshAI GACNNNNGTC 2 cut(s) 768, 1078
Psp124BI GAGCTC 1 cut(s) 550
Psp6I CCWGG 2 cut(s) 967, 1075
PspEI GGTNACC 1 cut(s) 204
PspGI CCWGG 2 cut(s) 967, 1075
PspN4I GGNNCC 1 cut(s) 619
PspXI VCTCGAGB 1 cut(s) 512
PsuI RGATCY 2 cut(s) 871, 1256
RsaI GTAC 3 cut(s) 167, 645, 988
RsaNI GTAC 3 cut(s) 166, 644, 987
SacI GAGCTC 1 cut(s) 550
SaqAI TTAA 7 cut(s) 84, 740, 747, 846, 876, 896, 1236
SatI GCNGC 5 cut(s) 5, 372, 539, 944, 1266
Sau3AI GATC 5 cut(s) 665, 685, 871, 1256, 1388
SchI GAGTC 5 cut(s) 23, 541, 778, 971, 1328
ScrFI CCNGG 2 cut(s) 969, 1077
SduI GDGCHC 3 cut(s) 550, 795, 905
SetI ASST 9 cut(s) 279, 326, 361, 457, 543, 550, 731, 792, 1054
SfaNI GCATC 2 cut(s) 128, 696
SfcI CTRYAG 1 cut(s) 447
Sfr274I CTCGAG 1 cut(s) 512
SlaI CTCGAG 1 cut(s) 512
SmlI CTYRAG 4 cut(s) 51, 512, 674, 895
SmoI CTYRAG 4 cut(s) 51, 512, 674, 895
SsiI CCGC 3 cut(s) 5, 372, 1373
SspMI CTAG 2 cut(s) 486, 1278
SstI GAGCTC 1 cut(s) 550
StyD4I CCNGG 2 cut(s) 967, 1075
StyI CCWWGG 3 cut(s) 91, 621, 1260
TaaI ACNGT 3 cut(s) 150, 986, 1086
TaqI TCGA 3 cut(s) 513, 867, 1072
TatI WGTACW 2 cut(s) 643, 986
TauI GCSGC 2 cut(s) 7, 374
TfiI GAWTC 7 cut(s) 76, 420, 565, 589, 808, 1060, 1069
Tru1I TTAA 7 cut(s) 84, 740, 747, 846, 876, 896, 1236
Tru9I TTAA 7 cut(s) 84, 740, 747, 846, 876, 896, 1236
TscAI CASTG 5 cut(s) 155, 524, 564, 913, 1062
TseFI GTSAC 3 cut(s) 204, 824, 1080
TseI GCWGC 3 cut(s) 538, 943, 1265
Tsp45I GTSAC 3 cut(s) 204, 824, 1080
TspDTI ATGAA 4 cut(s) 363, 366, 1053, 1188
TspGWI ACGGA 1 cut(s) 678
TspRI CASTG 5 cut(s) 155, 524, 564, 913, 1062
Vha464I CTTAAG 1 cut(s) 895
XagI CCTNNNNNAGG 1 cut(s) 1098
XapI RAATTY 5 cut(s) 125, 178, 1272, 1286, 1306
XceI RCATGY 1 cut(s) 223
XhoI CTCGAG 1 cut(s) 512
XspI CTAG 2 cut(s) 486, 1278
Zsp2I ATGCAT 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.