AT1G22460

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014932
Physical Location & Seq
Reverse (-)
7927287 .. 7930657
3371 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G22460.1

Sequence Viewer

Length: 1698 bp
ATGCGGCGGCGGAGGAAGACGGTTGTGGTGGCGGTAGTGATACGGAGGGTGCTAATATGGGCCATATGCGTAATGACATTACTCTGTTTTCTCACCGTACACATCTATGTTGCTCCTTTCAATAGACTCCCTAAGCTTCACCTTAACCACCACAACACTCGCCGAGGATATATCATAGATTACAATAAATCCATCACAGAACAGAGTTTAACGAGGAATCTGTCTCGTCCGGAATCTAATGAAATTCCTCTCATCTCCAATCCCAAGACAAATGAGATTCAGTATCAAAGTTCGATCTCGGAACACATCAACAACACCGAGCTAGTTCCTCCTCATGTGTCTACAAGCCCTTCTTCTTCTTCCAAGTTGAACATTACAAGCGGGATACCGGATTTCGACAAGCTTTGGAAACATCCACCGAACCGAAATTTCGTGCCTTGTGTCAGTCCCAATCCTAGTTACACATCTCCATTGGAATCTAGAGGCTATCTTCTTGTTCACACGAATGGCGGGCTTAACCAAATGCGCGCTGGGATATGTGACATGGTAGCTATAGCTCGGATTATAAATGCAACACTTGTAGTCCCCGAGCTCGACAAACGATCTTTCTGGCAGGATACTAGCAAATTCTCGGATGTTTTTGATGAAGATCACTTCATCAATGCTTTATCAAAAGATATAAGAGTCATCAAGAAGCTCCCAAAAGGCATTGATGGATTAACAAAGGTGGTTAAGCATTTCAAAAGCTACTCAGGATTGAGTTACTACCAAAATGAGATAGCTAGTATGTGGGATGAATATAAAGTGATCCGAGCCGCGAAATCCGATTCTCGTCTAGCAAACAACAACCTACCTCCTGATATTCAGAAGCTGCGATGTCGTGCTTGTTATGAGGCTCTACGCTTCTCTACTAAAATCAGATCAATGGGAGAACTTTTGGTCGATAGGATGAGGTCTTATGGTCTATACATCGCATTGCATTTGAGGTTCGAGAAAGAGATGCTAGCATTCAGCGGCTGCAACCATGGTCTATCCGCTTCTGAAGCTGCTGAGCTAAGGAGGATAAGGAAGAACACAGCGTATTGGAAAGTGAAAGACATTGACGGAAGAGTACAAAGACTCAAAGGCTATTGTCCATTGACTCCAAAAGAAGTTGGGATATTGCTAACAGCTCTTGGATATTCATCAGACACACCGGTATATATAGCTGCTGGTGAGATTTACGGTGGTGAGTCTCGGTTAGCCGATTTACGCTCTCGGTTCTCCATGTTAATGAGTAAGGAAAAATTGGCAACAAGAGAAGAGCTTAAGACTTTTATGAACCACTCAACTCAAATGGCTGCACTTGACTACATTGTTTCCATCGAAAGCGATGTTTTTATTCCGTCGTATAGTGGAAACATGGCGAGAGCTGTTGAAGGGCATCGTCGGTTTCTTGGTCACCGGAAAACTATCTCTCCGGACAGGAAAGCCATTGTAAGGTTGGTAGACAGAATTGGAAGAGGAGCTGAGAAAGATAACCGAAAAGTGTATGAAAGAATCAATGAGATTCACAAAACCCGACAAGGATCACCGAGGAGGAGAAAAGGGCCAGCTTCTGGAACAAAAGGTTTGGAGAGACATCGTTCTGAAGAATCCTTCTACGAGAACCCATTACCAGACTGTCTCTGCCAGAGAGACCCAAGTAAAGCGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

565

Amino Acids

64.51

Weight (kDa)

9.78

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 163 - 478 1.3e-101 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014932)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22460
fragaria_vesca FvH4_5g36600
malus_domestica MD08G1188100.v1.1 MD15G1374100.v1.1
pyrus_communis pycom08g16150 pycom15g33520
rosa_chinensis RchiOBHm_Chr7g0242381
rosa_laevigata RLG00000000897
rosa_multiflora Rmu_sc0000429.1_g000056
rosa_roxburghii Rroxscaffold_3G00222720
rosa_rugosa Rorug07G0315100
rosa_samantha Rh7AG470600 Rh7BG440500 Rh7CG487200 Rh7DG455700
rosa_wichuraiana Rw0G009510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 566
AccB7I CCANNNNNTGG 1 cut(s) 1598
AccI GTMKAC 2 cut(s) 341, 1488
AccII CGCG 2 cut(s) 528, 818
AccIII TCCGGA 2 cut(s) 229, 1459
AciI CCGC 9 cut(s) 4, 7, 10, 32, 381, 510, 816, 1014, 1035
AclWI GGATC 2 cut(s) 802, 1576
AcsI RAATTY 3 cut(s) 243, 427, 626
AcuI CTGAAG 2 cut(s) 1062, 1650
AfaI GTAC 2 cut(s) 99, 1113
AfiI CCNNNNNNNGG 2 cut(s) 1479, 1598
AflII CTTAAG 1 cut(s) 1307
AgeI ACCGGT 1 cut(s) 1195
AgsI TTSAA 4 cut(s) 121, 370, 742, 1418
Alw21I GWGCWC 1 cut(s) 594
Alw26I GTCTC 5 cut(s) 228, 1239, 1612, 1670, 1671
AlwI GGATC 2 cut(s) 802, 1576
AlwNI CAGNNNCTG 3 cut(s) 871, 1017, 1598
Ama87I CYCGRG 1 cut(s) 587
Aor13HI TCCGGA 2 cut(s) 229, 1459
AoxI GGCC 2 cut(s) 60, 1589
ApeKI GCWGC 5 cut(s) 871, 1017, 1046, 1208, 1340
ApoI RAATTY 3 cut(s) 243, 427, 626
AsiGI ACCGGT 1 cut(s) 1195
AspLEI GCGC 2 cut(s) 528, 530
AspS9I GGNCC 2 cut(s) 60, 1589
AsuHPI GGTGA 6 cut(s) 85, 131, 1226, 1241, 1433, 1563
AsuNHI GCTAGC 1 cut(s) 1003
AvaI CYCGRG 1 cut(s) 587
BanII GRGCYC 1 cut(s) 594
BbsI GAAGAC 1 cut(s) 23
Bbv12I GWGCWC 1 cut(s) 594
BbvI GCAGC 5 cut(s) 858, 1004, 1033, 1195, 1327
BccI CCATC 3 cut(s) 200, 707, 1370
BciVI GTATCC 2 cut(s) 378, 610
BcoDI GTCTC 5 cut(s) 228, 1239, 1612, 1670, 1671
BfaI CTAG 7 cut(s) 323, 456, 480, 621, 783, 836, 1004
BfmI CTRYAG 1 cut(s) 552
BfrI CTTAAG 1 cut(s) 1307
BfuI GTATCC 2 cut(s) 378, 610
BisI GCNGC 9 cut(s) 5, 8, 816, 872, 1015, 1018, 1047, 1209, 1341
BlpI GCTNAGC 1 cut(s) 1050
BlsI GCNGC 9 cut(s) 6, 9, 817, 873, 1016, 1019, 1048, 1210, 1342
BmeT110I CYCGRG 1 cut(s) 587
BmgT120I GGNCC 2 cut(s) 60, 1589
BmsI GCATC 2 cut(s) 990, 1432
BmtI GCTAGC 1 cut(s) 1007
BpiI GAAGAC 1 cut(s) 23
Bpu10I CCTNAGC 2 cut(s) 132, 1055
Bpu1102I GCTNAGC 1 cut(s) 1050
BsaBI GATNNNNATC 2 cut(s) 648, 1183
BsaI GGTCTC 1 cut(s) 1671
BsaJI CCNNGG 3 cut(s) 163, 1024, 1574
BsaWI WCCGGW 5 cut(s) 229, 388, 1195, 1443, 1459
BsaXI ACNNNNNCTCC 2 cut(s) 1441, 1471
Bsc4I CCNNNNNNNGG 2 cut(s) 1479, 1598
Bse118I RCCGGY 1 cut(s) 1195
Bse3DI GCAATG 1 cut(s) 974
Bse8I GATNNNNATC 2 cut(s) 648, 1183
BseAI TCCGGA 2 cut(s) 229, 1459
BseDI CCNNGG 3 cut(s) 163, 1024, 1574
BseGI GGATG 4 cut(s) 412, 640, 799, 954
BseJI GATNNNNATC 2 cut(s) 648, 1183
BseLI CCNNNNNNNGG 2 cut(s) 1479, 1598
BseMI GCAATG 1 cut(s) 974
BseMII CTCAG 3 cut(s) 765, 1041, 1500
BsePI GCGCGC 1 cut(s) 526
BseRI GAGGAG 4 cut(s) 321, 1518, 1591, 1594
BseXI GCAGC 5 cut(s) 858, 1004, 1033, 1195, 1327
BseYI CCCAGC 1 cut(s) 530
BsgI GTGCAG 1 cut(s) 1326
Bsh1236I CGCG 2 cut(s) 528, 818
BshFI GGCC 2 cut(s) 62, 1591
BshTI ACCGGT 1 cut(s) 1195
BsiHKAI GWGCWC 1 cut(s) 594
BsiHKCI CYCGRG 1 cut(s) 587
BsiSI CCGG 5 cut(s) 230, 389, 1196, 1444, 1460
BslFI GGGAC 2 cut(s) 432, 569
BslI CCNNNNNNNGG 2 cut(s) 1479, 1598
BsmAI GTCTC 5 cut(s) 228, 1239, 1612, 1670, 1671
BsmFI GGGAC 2 cut(s) 432, 569
BsmI GAATGC 1 cut(s) 1007
BsnI GGCC 2 cut(s) 62, 1591
Bso31I GGTCTC 1 cut(s) 1671
BsoBI CYCGRG 1 cut(s) 587
Bsp1286I GDGCHC 1 cut(s) 594
Bsp13I TCCGGA 2 cut(s) 229, 1459
Bsp143I GATC 6 cut(s) 294, 602, 649, 807, 920, 1568
Bsp1720I GCTNAGC 1 cut(s) 1050
Bsp19I CCATGG 1 cut(s) 1024
BspACI CCGC 9 cut(s) 4, 7, 10, 32, 381, 510, 816, 1014, 1035
BspANI GGCC 2 cut(s) 62, 1591
BspCNI CTCAG 3 cut(s) 764, 1042, 1501
BspEI TCCGGA 2 cut(s) 229, 1459
BspFNI CGCG 2 cut(s) 528, 818
BspOI GCTAGC 1 cut(s) 1007
BspPI GGATC 2 cut(s) 802, 1576
BspQI GCTCTTC 1 cut(s) 1296
BspTI CTTAAG 1 cut(s) 1307
BspTNI GGTCTC 1 cut(s) 1671
BsrDI GCAATG 1 cut(s) 974
BsrFI RCCGGY 1 cut(s) 1195
BssAI RCCGGY 1 cut(s) 1195
BssECI CCNNGG 3 cut(s) 163, 1024, 1574
BssHII GCGCGC 1 cut(s) 526
BssMI GATC 6 cut(s) 294, 602, 649, 807, 920, 1568
BssT1I CCWWGG 1 cut(s) 1024
Bst4CI ACNGT 4 cut(s) 22, 97, 1226, 1664
Bst6I CTCTTC 3 cut(s) 1102, 1296, 1495
BstAFI CTTAAG 1 cut(s) 1307
BstC8I GCNNGC 4 cut(s) 512, 528, 1005, 1593
BstDEI CTNAG 5 cut(s) 132, 751, 1050, 1055, 1509
BstDSI CCRYGG 1 cut(s) 1024
BstEII GGTNACC 1 cut(s) 1439
BstF5I GGATG 4 cut(s) 412, 640, 799, 954
BstFNI CGCG 2 cut(s) 528, 818
BstHHI GCGC 2 cut(s) 528, 530
BstKTI GATC 6 cut(s) 297, 605, 652, 810, 923, 1571
BstMAI GTCTC 5 cut(s) 228, 1239, 1612, 1670, 1671
BstMBI GATC 6 cut(s) 294, 602, 649, 807, 920, 1568
BstMWI GCNNNNNNNGC 1 cut(s) 1043
BstPI GGTNACC 1 cut(s) 1439
BstSFI CTRYAG 1 cut(s) 552
BstUI CGCG 2 cut(s) 528, 818
BstV1I GCAGC 5 cut(s) 858, 1004, 1033, 1195, 1327
BstV2I GAAGAC 1 cut(s) 23
BsuI GTATCC 2 cut(s) 378, 610
BsuRI GGCC 2 cut(s) 62, 1591
BtgI CCRYGG 1 cut(s) 1024
BtgZI GCGATG 3 cut(s) 889, 955, 1386
BtsCI GGATG 4 cut(s) 412, 640, 799, 954
Cac8I GCNNGC 4 cut(s) 512, 528, 1005, 1593
CaiI CAGNNNCTG 3 cut(s) 871, 1017, 1598
CfoI GCGC 2 cut(s) 528, 530
Cfr10I RCCGGY 1 cut(s) 1195
Cfr13I GGNCC 2 cut(s) 60, 1589
Csp6I GTAC 2 cut(s) 98, 1112
CspAI ACCGGT 1 cut(s) 1195
CviAII CATG 5 cut(s) 335, 544, 1025, 1267, 1402
CviQI GTAC 2 cut(s) 98, 1112
DdeI CTNAG 5 cut(s) 132, 751, 1050, 1055, 1509
DpnI GATC 6 cut(s) 296, 604, 651, 809, 922, 1570
DpnII GATC 6 cut(s) 294, 602, 649, 807, 920, 1568
Eam1104I CTCTTC 3 cut(s) 1102, 1296, 1495
EarI CTCTTC 3 cut(s) 1102, 1296, 1495
EciI GGCGGA 1 cut(s) 25
Ecl136II GAGCTC 1 cut(s) 592
Eco130I CCWWGG 1 cut(s) 1024
Eco24I GRGCYC 1 cut(s) 594
Eco31I GGTCTC 1 cut(s) 1671
Eco53kI GAGCTC 1 cut(s) 592
Eco57I CTGAAG 2 cut(s) 1062, 1650
Eco88I CYCGRG 1 cut(s) 587
Eco91I GGTNACC 1 cut(s) 1439
EcoICRI GAGCTC 1 cut(s) 592
EcoO65I GGTNACC 1 cut(s) 1439
EcoT14I CCWWGG 1 cut(s) 1024
EcoT38I GRGCYC 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 1024
FaeI CATG 5 cut(s) 338, 547, 1028, 1270, 1405
FalI AAGNNNNNCTT 2 cut(s) 337, 369
FaqI GGGAC 2 cut(s) 432, 569
FatI CATG 5 cut(s) 334, 543, 1024, 1266, 1401
FauI CCCGC 2 cut(s) 374, 503
FauNDI CATATG 1 cut(s) 65
FblI GTMKAC 2 cut(s) 341, 1488
Fnu4HI GCNGC 9 cut(s) 5, 8, 816, 872, 1015, 1018, 1047, 1209, 1341
FokI GGATG 4 cut(s) 399, 647, 806, 961
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 9 cut(s) 5, 8, 816, 872, 1015, 1018, 1047, 1209, 1341
FspBI CTAG 7 cut(s) 323, 456, 480, 621, 783, 836, 1004
GlaI GCGC 2 cut(s) 527, 529
GluI GCNGC 9 cut(s) 5, 8, 816, 872, 1015, 1018, 1047, 1209, 1341
GsaI CCCAGC 1 cut(s) 534
HaeIII GGCC 2 cut(s) 62, 1591
HapII CCGG 5 cut(s) 230, 389, 1196, 1444, 1460
HhaI GCGC 2 cut(s) 528, 530
Hin1II CATG 5 cut(s) 338, 547, 1028, 1270, 1405
Hin6I GCGC 2 cut(s) 526, 528
HinP1I GCGC 2 cut(s) 526, 528
HindIII AAGCTT 2 cut(s) 134, 401
HpaII CCGG 5 cut(s) 230, 389, 1196, 1444, 1460
HphI GGTGA 6 cut(s) 85, 131, 1226, 1241, 1433, 1563
Hpy166II GTNNAC 4 cut(s) 100, 342, 499, 1489
Hpy188III TCNNGA 8 cut(s) 230, 480, 691, 753, 857, 991, 1460, 1599
Hpy8I GTNNAC 4 cut(s) 100, 342, 499, 1489
Hpy99I CGWCG 2 cut(s) 1390, 1431
HpyAV CCTTC 3 cut(s) 360, 1412, 1648
HpyCH4III ACNGT 4 cut(s) 22, 97, 1226, 1664
HpyCH4V TGCA 4 cut(s) 572, 979, 1020, 1343
HpyF10VI GCNNNNNNNGC 1 cut(s) 1043
HpyF3I CTNAG 5 cut(s) 132, 751, 1050, 1055, 1509
Hsp92II CATG 5 cut(s) 338, 547, 1028, 1270, 1405
HspAI GCGC 2 cut(s) 526, 528
Kpn2I TCCGGA 2 cut(s) 229, 1459
Kzo9I GATC 6 cut(s) 294, 602, 649, 807, 920, 1568
LguI GCTCTTC 1 cut(s) 1296
LmnI GCTCC 3 cut(s) 118, 702, 1505
Lsp1109I GCAGC 5 cut(s) 858, 1004, 1033, 1195, 1327
LweI GCATC 2 cut(s) 990, 1432
MaeI CTAG 7 cut(s) 323, 456, 480, 621, 783, 836, 1004
MaeIII GTNAC 4 cut(s) 458, 539, 761, 1439
MalI GATC 6 cut(s) 296, 604, 651, 809, 922, 1570
MboI GATC 6 cut(s) 294, 602, 649, 807, 920, 1568
MhlI GDGCHC 1 cut(s) 594
MluCI AATT 5 cut(s) 243, 427, 626, 1286, 1494
MlyI GAGTC 5 cut(s) 120, 693, 1113, 1135, 1241
MroI TCCGGA 2 cut(s) 229, 1459
MseI TTAA 7 cut(s) 144, 209, 516, 719, 732, 1271, 1308
MslI CAYNNNNRTG 3 cut(s) 105, 504, 1271
MspA1I CMGCKG 1 cut(s) 1014
MspCI CTTAAG 1 cut(s) 1307
MspI CCGG 5 cut(s) 230, 389, 1196, 1444, 1460
Mva1269I GAATGC 1 cut(s) 1007
MvnI CGCG 2 cut(s) 528, 818
MwoI GCNNNNNNNGC 1 cut(s) 1043
NcoI CCATGG 1 cut(s) 1024
NdeI CATATG 1 cut(s) 65
NdeII GATC 6 cut(s) 294, 602, 649, 807, 920, 1568
NheI GCTAGC 1 cut(s) 1003
NlaIII CATG 5 cut(s) 338, 547, 1028, 1270, 1405
NmeAIII GCCGAG 1 cut(s) 188
NmuCI GTSAC 2 cut(s) 539, 1439
PauI GCGCGC 1 cut(s) 526
PciSI GCTCTTC 1 cut(s) 1296
PctI GAATGC 1 cut(s) 1007
PfeI GAWTC 8 cut(s) 217, 233, 277, 476, 827, 1539, 1549, 1634
PflMI CCANNNNNTGG 1 cut(s) 1598
PinAI ACCGGT 1 cut(s) 1195
PkrI GCNGC 9 cut(s) 6, 9, 817, 873, 1016, 1019, 1048, 1210, 1342
PleI GAGTC 5 cut(s) 120, 692, 1113, 1135, 1240
PpsI GAGTC 5 cut(s) 120, 692, 1113, 1135, 1240
PsiI TTATAA 1 cut(s) 566
Psp124BI GAGCTC 1 cut(s) 594
PspEI GGTNACC 1 cut(s) 1439
PspFI CCCAGC 1 cut(s) 530
PspPI GGNCC 2 cut(s) 60, 1589
PstNI CAGNNNCTG 3 cut(s) 871, 1017, 1598
PteI GCGCGC 1 cut(s) 526
RsaI GTAC 2 cut(s) 99, 1113
RsaNI GTAC 2 cut(s) 98, 1112
RseI CAYNNNNRTG 3 cut(s) 105, 504, 1271
SacI GAGCTC 1 cut(s) 594
SapI GCTCTTC 1 cut(s) 1296
SaqAI TTAA 7 cut(s) 144, 209, 516, 719, 732, 1271, 1308
SatI GCNGC 9 cut(s) 5, 8, 816, 872, 1015, 1018, 1047, 1209, 1341
Sau3AI GATC 6 cut(s) 294, 602, 649, 807, 920, 1568
Sau96I GGNCC 2 cut(s) 60, 1589
SchI GAGTC 5 cut(s) 120, 693, 1113, 1135, 1241
SduI GDGCHC 1 cut(s) 594
SfaNI GCATC 2 cut(s) 990, 1432
SfcI CTRYAG 1 cut(s) 552
SmiMI CAYNNNNRTG 3 cut(s) 105, 504, 1271
SmlI CTYRAG 1 cut(s) 1307
SmoI CTYRAG 1 cut(s) 1307
Sse9I AATT 5 cut(s) 243, 427, 626, 1286, 1494
SsiI CCGC 9 cut(s) 4, 7, 10, 32, 381, 510, 816, 1014, 1035
SspMI CTAG 7 cut(s) 323, 456, 480, 621, 783, 836, 1004
SstI GAGCTC 1 cut(s) 594
StyI CCWWGG 1 cut(s) 1024
TaaI ACNGT 4 cut(s) 22, 97, 1226, 1664
TaqI TCGA 6 cut(s) 293, 396, 594, 942, 990, 1365
TasI AATT 5 cut(s) 243, 427, 626, 1286, 1494
TatI WGTACW 1 cut(s) 1111
TauI GCSGC 4 cut(s) 7, 10, 818, 1017
TfiI GAWTC 8 cut(s) 217, 233, 277, 476, 827, 1539, 1549, 1634
Tru1I TTAA 7 cut(s) 144, 209, 516, 719, 732, 1271, 1308
Tru9I TTAA 7 cut(s) 144, 209, 516, 719, 732, 1271, 1308
TseFI GTSAC 2 cut(s) 539, 1439
TseI GCWGC 5 cut(s) 871, 1017, 1046, 1208, 1340
Tsp45I GTSAC 2 cut(s) 539, 1439
TspDTI ATGAA 7 cut(s) 255, 646, 660, 810, 1173, 1334, 1548
TspGWI ACGGA 3 cut(s) 58, 1119, 1374
Van91I CCANNNNNTGG 1 cut(s) 1598
Vha464I CTTAAG 1 cut(s) 1307
XapI RAATTY 3 cut(s) 243, 427, 626
XbaI TCTAGA 1 cut(s) 479
XcmI CCANNNNNNNNNTGG 2 cut(s) 527, 1480
XmiI GTMKAC 2 cut(s) 341, 1488
XspI CTAG 7 cut(s) 323, 456, 480, 621, 783, 836, 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.