MD08G1188100.v1.1

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014932
Physical Location & Seq
Reverse (-)
23649111 .. 23659709
10599 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1188100.v1.1.491

Sequence Viewer

Length: 1587 bp
ATGCAGAAGAAGAGGTCGGTAGCGTTGGGCTCACTGAGGAAAGTGCTAACGTGCGCCATATGCTCAATAGCGCTAGTGGCTCTCTTCTCCGTCCACGTACCTATCTTCCCTTCTTCAAGGGTCCATGACTTCTCTGACCCCGTCAAGCTCCCCACGCAACATGATATTAAATACCAGAAGTTGAGCGCCGAGCAGAGCTGGACACTGGAGATCGCTCCGCCCCATCTGTTGAAAACGCCTCTGCCTTCTAGTTCTGGCAAGTTAGAAGGTTCAAGTGGGATTTTGGAAACTGACAAGCTATGGAAGCCTCCATCAAATCGTGATTTTGTGCCATGCGTAGAGCCAAGTCCCAATTATACATCTCCTACAGAGTCACGGGGCTACCTGCTAGTTAATACAAATGGTGGGCTCAACCAGATGCGTGCTGGGATATGTGACATGGTAGCAGTAGCCCGTATCATAAATGCTACTCTTGTAATTCCGGAACTTGATAAACAGTCATTTTGGAATGACTCTAGCAACTTCTCAGATGTTTTTGATGAAGATTTTTTTATCAATGCTTTAGCCAATGATGTCAAAATTATAAAAATCCTTCCTTCGGAACTAGCTACTGGTATAAGAGCGGTTAAGCATTTTAGAAGCTGGTCTGGTATGGATTATTACCAAGATGAGATAGCTGGCCTCTGGGAAGAATACGAGGTTATTCGAGCTGCCAAATCTGATTCTCGCTTGGCAAATAACAACCTACCTTTAGATATACAGAAGCTTCGCTGCCGTGCTTGTTATGAAGCTCTACGTTTTGCACCTCAAATTGAAGCAATGGGAAAACTGTTAGTTGATCGAATGAGGTCGTTTGGTCCTTATATTGCTCTGCATTTACGATTTGAGGAGGACATGCTTGCCTTTAGTGGATGCACTAAAGATTTATCTCCAGATGAATCTGAGGAACTAAGGATAATTCGAGAGAATACCCCATACTGGAAAGAGAAGGAAATTGATGCCGCAGAACGGAGATCCAAAGGGTATTGCCCCCTAACACCAAAGGAGGTTGGAATTTTTCTCAACGCTCTTGGGTACCCCTCAAACACACCTATATATCTTGCTGCTGGGAAGATATATGGGGGCGAATCTCATATGGCTGCTCTGCGGTCACGTTATCCAATACTGCTGAGCAAGGAAACTTTGGCATCCGCCGAGGAGCTTGAACCCTTTACCAATCATGCATCTCAAATGGCTGCGCTTGACTACATTGTTTCAGTTGAAAGTGATGTATTTATACCTTCATACTCTGGAAACATGGCAAGAGCAGTTGAAGGTCATCGACGATTTCTTGGACACAGGAAAACAATATCTCCTGACAGAAAAGCCCTTGTTCGCCTGATTGACAAACTTCAGCAGGGAACTCTGAAGGAGGGCAAAAACCTATCAAATAGAGTTATTGAAATGCACATGAAACGGCAAGGCTCCGCGCGGAAGAGAAAGGGTCCCATTTCAGGAACTAAGGGCACAGAAAGGTTTCGTTCAGAAGAACCCTTTTACGTGAACCCTTTACCAGATTGTTTGTGTAAGAAGGAATTCCCAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

529

Amino Acids

59.38

Weight (kDa)

8.62

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 128 - 443 1.4e-103 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014932)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22460
fragaria_vesca FvH4_5g36600
malus_domestica MD08G1188100.v1.1 MD15G1374100.v1.1
pyrus_communis pycom08g16150 pycom15g33520
rosa_chinensis RchiOBHm_Chr7g0242381
rosa_laevigata RLG00000000897
rosa_multiflora Rmu_sc0000429.1_g000056
rosa_roxburghii Rroxscaffold_3G00222720
rosa_rugosa Rorug07G0315100
rosa_samantha Rh7AG470600 Rh7BG440500 Rh7CG487200 Rh7DG455700
rosa_wichuraiana Rw0G009510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
Acc36I ACCTGC 1 cut(s) 393
Acc65I GGTACC 1 cut(s) 1074
AccB1I GGYRCC 1 cut(s) 1074
AccBSI CCGCTC 1 cut(s) 623
AccII CGCG 2 cut(s) 1469, 1471
AccIII TCCGGA 1 cut(s) 481
AciI CCGC 7 cut(s) 218, 623, 1002, 1147, 1191, 1467, 1471
AclWI GGATC 1 cut(s) 1008
AcsI RAATTY 2 cut(s) 1053, 1574
AcuI CTGAAG 2 cut(s) 1376, 1427
AfaI GTAC 2 cut(s) 99, 1076
AfeI AGCGCT 1 cut(s) 72
AfiI CCNNNNNNNGG 4 cut(s) 598, 978, 1008, 1493
AgsI TTSAA 8 cut(s) 117, 232, 273, 815, 1205, 1262, 1313, 1442
AlwI GGATC 1 cut(s) 1008
Aor13HI TCCGGA 1 cut(s) 481
Aor51HI AGCGCT 1 cut(s) 72
AoxI GGCC 1 cut(s) 679
ApeKI GCWGC 5 cut(s) 710, 771, 1103, 1139, 1235
ApoI RAATTY 2 cut(s) 1053, 1574
Asp700I GAANNNNTTC 2 cut(s) 1515, 1574
Asp718I GGTACC 1 cut(s) 1074
AspLEI GCGC 5 cut(s) 56, 73, 188, 1240, 1471
AspS9I GGNCC 3 cut(s) 121, 857, 1484
AvaII GGWCC 3 cut(s) 121, 857, 1484
BaeGI GKGCMC 1 cut(s) 1508
BanI GGYRCC 1 cut(s) 1074
BanII GRGCYC 2 cut(s) 32, 411
BbvI GCAGC 5 cut(s) 697, 758, 1090, 1126, 1222
BccI CCATC 2 cut(s) 231, 319
BceAI ACGGC 2 cut(s) 759, 1472
BfaI CTAG 5 cut(s) 74, 249, 389, 516, 605
BfmI CTRYAG 1 cut(s) 366
BfoI RGCGCY 2 cut(s) 74, 189
BfuAI ACCTGC 1 cut(s) 393
BisI GCNGC 6 cut(s) 711, 772, 1002, 1104, 1140, 1236
BlpI GCTNAGC 1 cut(s) 1169
BlsI GCNGC 6 cut(s) 712, 773, 1003, 1105, 1141, 1237
Bme18I GGWCC 3 cut(s) 121, 857, 1484
BmgT120I GGNCC 3 cut(s) 121, 857, 1484
BmiI GGNNCC 5 cut(s) 122, 1076, 1465, 1485, 1486
BmsI GCATC 5 cut(s) 408, 902, 988, 1196, 1232
BpmI CTGGAG 2 cut(s) 227, 915
Bpu1102I GCTNAGC 1 cut(s) 1169
BsaAI YACGTR 2 cut(s) 97, 1540
BsaJI CCNNGG 1 cut(s) 1194
BsaWI WCCGGW 1 cut(s) 481
BsaXI ACNNNNNCTCC 2 cut(s) 1336, 1366
Bsc4I CCNNNNNNNGG 4 cut(s) 598, 978, 1008, 1493
Bse1I ACTGG 3 cut(s) 210, 616, 983
Bse3DI GCAATG 1 cut(s) 825
BseAI TCCGGA 1 cut(s) 481
BseDI CCNNGG 1 cut(s) 1194
BseGI GGATG 2 cut(s) 917, 1187
BseLI CCNNNNNNNGG 4 cut(s) 598, 978, 1008, 1493
BseMI GCAATG 1 cut(s) 825
BseMII CTCAG 4 cut(s) 26, 540, 933, 1160
BseNI ACTGG 3 cut(s) 210, 616, 983
BseRI GAGGAG 2 cut(s) 902, 1211
BseSI GKGCMC 1 cut(s) 1508
BseXI GCAGC 5 cut(s) 697, 758, 1090, 1126, 1222
BseYI CCCAGC 2 cut(s) 425, 1106
Bsh1236I CGCG 2 cut(s) 1469, 1471
BshFI GGCC 1 cut(s) 681
BshNI GGYRCC 1 cut(s) 1074
BsiSI CCGG 1 cut(s) 482
BslFI GGGAC 2 cut(s) 333, 1470
BslI CCNNNNNNNGG 4 cut(s) 598, 978, 1008, 1493
BsmFI GGGAC 2 cut(s) 333, 1470
BsnI GGCC 1 cut(s) 681
Bsp1286I GDGCHC 3 cut(s) 32, 411, 1508
Bsp13I TCCGGA 1 cut(s) 481
Bsp143I GATC 3 cut(s) 210, 838, 1013
Bsp1720I GCTNAGC 1 cut(s) 1169
BspACI CCGC 7 cut(s) 218, 623, 1002, 1147, 1191, 1467, 1471
BspANI GGCC 1 cut(s) 681
BspCNI CTCAG 4 cut(s) 27, 539, 934, 1161
BspEI TCCGGA 1 cut(s) 481
BspFNI CGCG 2 cut(s) 1469, 1471
BspLI GGNNCC 5 cut(s) 122, 1076, 1465, 1485, 1486
BspMI ACCTGC 1 cut(s) 393
BspPI GGATC 1 cut(s) 1008
BspT107I GGYRCC 1 cut(s) 1074
BsrBI CCGCTC 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 825
BsrI ACTGG 3 cut(s) 210, 616, 983
BssECI CCNNGG 1 cut(s) 1194
BssMI GATC 3 cut(s) 210, 838, 1013
Bst4CI ACNGT 2 cut(s) 498, 831
Bst6I CTCTTC 3 cut(s) 5, 89, 1469
BstBAI YACGTR 2 cut(s) 97, 1540
BstC8I GCNNGC 3 cut(s) 423, 679, 900
BstDEI CTNAG 6 cut(s) 35, 526, 942, 950, 1169, 1500
BstF5I GGATG 2 cut(s) 917, 1187
BstFNI CGCG 2 cut(s) 1469, 1471
BstH2I RGCGCY 2 cut(s) 74, 189
BstHHI GCGC 5 cut(s) 56, 73, 188, 1240, 1471
BstKTI GATC 3 cut(s) 213, 841, 1016
BstMBI GATC 3 cut(s) 210, 838, 1013
BstMWI GCNNNNNNNGC 4 cut(s) 60, 77, 154, 304
BstNSI RCATGY 1 cut(s) 898
BstSFI CTRYAG 1 cut(s) 366
BstSLI GKGCMC 1 cut(s) 1508
BstUI CGCG 2 cut(s) 1469, 1471
BstV1I GCAGC 5 cut(s) 697, 758, 1090, 1126, 1222
BstX2I RGATCY 1 cut(s) 1013
BstYI RGATCY 1 cut(s) 1013
BsuRI GGCC 1 cut(s) 681
BtsCI GGATG 2 cut(s) 917, 1187
BtsIMutI CAGTG 2 cut(s) 32, 203
BveI ACCTGC 1 cut(s) 393
Cac8I GCNNGC 3 cut(s) 423, 679, 900
CfoI GCGC 5 cut(s) 56, 73, 188, 1240, 1471
Cfr13I GGNCC 3 cut(s) 121, 857, 1484
Csp6I GTAC 2 cut(s) 98, 1075
CviAII CATG 8 cut(s) 125, 161, 333, 439, 895, 1220, 1297, 1450
CviQI GTAC 2 cut(s) 98, 1075
DdeI CTNAG 6 cut(s) 35, 526, 942, 950, 1169, 1500
DpnI GATC 3 cut(s) 212, 840, 1015
DpnII GATC 3 cut(s) 210, 838, 1013
Eam1104I CTCTTC 3 cut(s) 5, 89, 1469
EarI CTCTTC 3 cut(s) 5, 89, 1469
EciI GGCGGA 2 cut(s) 207, 1180
Eco24I GRGCYC 2 cut(s) 32, 411
Eco47I GGWCC 3 cut(s) 121, 857, 1484
Eco47III AGCGCT 1 cut(s) 72
Eco57I CTGAAG 2 cut(s) 1376, 1427
EcoO109I RGGNCCY 1 cut(s) 1484
EcoRI GAATTC 1 cut(s) 1574
EcoT22I ATGCAT 1 cut(s) 1225
EcoT38I GRGCYC 2 cut(s) 32, 411
FaeI CATG 8 cut(s) 128, 164, 336, 442, 898, 1223, 1300, 1453
FaqI GGGAC 2 cut(s) 333, 1470
FatI CATG 8 cut(s) 124, 160, 332, 438, 894, 1219, 1296, 1449
FauNDI CATATG 2 cut(s) 59, 1134
Fnu4HI GCNGC 6 cut(s) 711, 772, 1002, 1104, 1140, 1236
FokI GGATG 2 cut(s) 924, 1174
FriOI GRGCYC 2 cut(s) 32, 411
Fsp4HI GCNGC 6 cut(s) 711, 772, 1002, 1104, 1140, 1236
FspBI CTAG 5 cut(s) 74, 249, 389, 516, 605
GlaI GCGC 5 cut(s) 55, 72, 187, 1239, 1470
GluI GCNGC 6 cut(s) 711, 772, 1002, 1104, 1140, 1236
GsaI CCCAGC 2 cut(s) 429, 1110
GsuI CTGGAG 2 cut(s) 227, 915
HaeII RGCGCY 2 cut(s) 74, 189
HaeIII GGCC 1 cut(s) 681
HapII CCGG 1 cut(s) 482
HhaI GCGC 5 cut(s) 56, 73, 188, 1240, 1471
Hin1II CATG 8 cut(s) 128, 164, 336, 442, 898, 1223, 1300, 1453
Hin6I GCGC 5 cut(s) 54, 71, 186, 1238, 1469
HinP1I GCGC 5 cut(s) 54, 71, 186, 1238, 1469
HindIII AAGCTT 1 cut(s) 764
HinfI GANTC 5 cut(s) 371, 512, 722, 938, 1127
HpaII CCGG 1 cut(s) 482
Hpy166II GTNNAC 2 cut(s) 94, 1543
Hpy188I TCNGA 7 cut(s) 136, 529, 601, 721, 943, 1407, 1525
Hpy188III TCNNGA 7 cut(s) 320, 482, 932, 962, 1290, 1355, 1494
Hpy8I GTNNAC 2 cut(s) 94, 1543
Hpy99I CGWCG 1 cut(s) 1326
HpyCH4III ACNGT 2 cut(s) 498, 831
HpyCH4IV ACGT 5 cut(s) 50, 96, 796, 1153, 1539
HpyCH4V TGCA 6 cut(s) 4, 803, 874, 915, 1223, 1447
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 77, 154, 304
HpyF3I CTNAG 6 cut(s) 35, 526, 942, 950, 1169, 1500
HpySE526I ACGT 5 cut(s) 50, 96, 796, 1153, 1539
Hsp92II CATG 8 cut(s) 128, 164, 336, 442, 898, 1223, 1300, 1453
HspAI GCGC 5 cut(s) 54, 71, 186, 1238, 1469
KflI GGGWCCC 1 cut(s) 1484
Kpn2I TCCGGA 1 cut(s) 481
KpnI GGTACC 1 cut(s) 1078
Kzo9I GATC 3 cut(s) 210, 838, 1013
LmnI GCTCC 4 cut(s) 153, 220, 1198, 1469
Lsp1109I GCAGC 5 cut(s) 697, 758, 1090, 1126, 1222
LweI GCATC 5 cut(s) 408, 902, 988, 1196, 1232
MaeI CTAG 5 cut(s) 74, 249, 389, 516, 605
MaeII ACGT 5 cut(s) 50, 96, 796, 1153, 1539
MaeIII GTNAC 3 cut(s) 372, 434, 1149
MalI GATC 3 cut(s) 212, 840, 1015
MbiI CCGCTC 1 cut(s) 623
MboI GATC 3 cut(s) 210, 838, 1013
MflI RGATCY 1 cut(s) 1013
MhlI GDGCHC 3 cut(s) 32, 411, 1508
MluCI AATT 8 cut(s) 352, 477, 579, 810, 957, 993, 1053, 1574
MlyI GAGTC 2 cut(s) 380, 506
MmeI TCCRAC 1 cut(s) 1030
Mph1103I ATGCAT 1 cut(s) 1225
MroI TCCGGA 1 cut(s) 481
MroXI GAANNNNTTC 2 cut(s) 1515, 1574
MseI TTAA 3 cut(s) 168, 393, 627
MspI CCGG 1 cut(s) 482
MvnI CGCG 2 cut(s) 1469, 1471
MwoI GCNNNNNNNGC 4 cut(s) 60, 77, 154, 304
NdeI CATATG 2 cut(s) 59, 1134
NdeII GATC 3 cut(s) 210, 838, 1013
NlaIII CATG 8 cut(s) 128, 164, 336, 442, 898, 1223, 1300, 1453
NlaIV GGNNCC 5 cut(s) 122, 1076, 1465, 1485, 1486
NmeAIII GCCGAG 2 cut(s) 214, 1219
NmuCI GTSAC 3 cut(s) 372, 434, 1149
NsiI ATGCAT 1 cut(s) 1225
NspI RCATGY 1 cut(s) 898
PdmI GAANNNNTTC 2 cut(s) 1515, 1574
PfeI GAWTC 3 cut(s) 722, 938, 1127
PflFI GACNNNGTC 1 cut(s) 140
PkrI GCNGC 6 cut(s) 712, 773, 1003, 1105, 1141, 1237
PleI GAGTC 2 cut(s) 379, 506
PpsI GAGTC 2 cut(s) 379, 506
Ppu21I YACGTR 2 cut(s) 97, 1540
PpuMI RGGWCCY 1 cut(s) 1484
PsiI TTATAA 1 cut(s) 584
Psp5II RGGWCCY 1 cut(s) 1484
PspFI CCCAGC 2 cut(s) 425, 1106
PspN4I GGNNCC 5 cut(s) 122, 1076, 1465, 1485, 1486
PspPI GGNCC 3 cut(s) 121, 857, 1484
PspPPI RGGWCCY 1 cut(s) 1484
PsuI RGATCY 1 cut(s) 1013
PsyI GACNNNGTC 1 cut(s) 140
RsaI GTAC 2 cut(s) 99, 1076
RsaNI GTAC 2 cut(s) 98, 1075
SaqAI TTAA 3 cut(s) 168, 393, 627
SatI GCNGC 6 cut(s) 711, 772, 1002, 1104, 1140, 1236
Sau3AI GATC 3 cut(s) 210, 838, 1013
Sau96I GGNCC 3 cut(s) 121, 857, 1484
SchI GAGTC 2 cut(s) 380, 506
SduI GDGCHC 3 cut(s) 32, 411, 1508
SfaNI GCATC 5 cut(s) 408, 902, 988, 1196, 1232
SfcI CTRYAG 1 cut(s) 366
SinI GGWCC 3 cut(s) 121, 857, 1484
Sse9I AATT 8 cut(s) 352, 477, 579, 810, 957, 993, 1053, 1574
SsiI CCGC 7 cut(s) 218, 623, 1002, 1147, 1191, 1467, 1471
SspMI CTAG 5 cut(s) 74, 249, 389, 516, 605
TaaI ACNGT 2 cut(s) 498, 831
TaiI ACGT 5 cut(s) 53, 99, 799, 1156, 1542
TaqI TCGA 4 cut(s) 706, 841, 961, 1321
TasI AATT 8 cut(s) 352, 477, 579, 810, 957, 993, 1053, 1574
TauI GCSGC 1 cut(s) 1004
TfiI GAWTC 3 cut(s) 722, 938, 1127
Tru1I TTAA 3 cut(s) 168, 393, 627
Tru9I TTAA 3 cut(s) 168, 393, 627
TscAI CASTG 2 cut(s) 39, 210
TseFI GTSAC 3 cut(s) 372, 434, 1149
TseI GCWGC 5 cut(s) 710, 771, 1103, 1139, 1235
Tsp45I GTSAC 3 cut(s) 372, 434, 1149
TspDTI ATGAA 5 cut(s) 555, 801, 951, 1272, 1466
TspGWI ACGGA 2 cut(s) 79, 1024
TspRI CASTG 2 cut(s) 39, 210
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 3 cut(s) 121, 857, 1484
XapI RAATTY 2 cut(s) 1053, 1574
XceI RCATGY 1 cut(s) 898
XcmI CCANNNNNNNNNTGG 1 cut(s) 422
XmnI GAANNNNTTC 2 cut(s) 1515, 1574
XspI CTAG 5 cut(s) 74, 249, 389, 516, 605
Zsp2I ATGCAT 1 cut(s) 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.