AT1G27461

maturation of SSU-rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016019
Physical Location & Seq
Reverse (-)
9537736 .. 9539027
1292 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27461.1

Sequence Viewer

Length: 1065 bp
ATGATAAAGATACTCAACCCCCACTCACACCATTCTCAAACCACAACTCTTAAAACAGCTGAGATCTTGTCTAAATACAGACCCATTGCACCAAAGCCCGGGACGCCTCGAGTGAACGACGATGATCCTTCTTCCTCTATGTCTCACAAAATCAGCCAATCTCCTTACCTTCGTAACCTCTGGCCTCAGCTTCAAGCGCGACCTACAAGAACCCGCAAGCGAGGCCGAGGCGGGATGGGTCCCTCTTCTCTTGCTATGAAGAGACCAAAGTCGTCATGCGGTTCTTCTAGTACTTCTACTATTTCTACACAACGTGTTCTTGGACCTATTAAAACACTGTCGTTTCAGGCTTTCACTCATCATAGGTTACCTAACCTACCTCAAGTTGGCTACGGTTTTGAGAATGGTGTCTCTTCTACTTTGGTGACTTTGCCTCTTCTCCAATGCTCTCCTCCTTCCTCCAAATGCATGGAGCCAGAGATCAAAGGAAAAGGTGTCATTGATCTGAACAAGACCGCGGAAGTGATACAGGAGAGAGACTTCTTGACGCAACTACAAGGACCCATCACCACAACCACAACCGCAACCACAAGCAGAGTCATATCACCGCAGCCCATAAGGCCGGTTTGCTCAAAGATCAACGTAGCCTACATAAACCCACTAACCAACCCATCTCCAACGTCTCAAACCAGCAAGAAATCACCAAGAGAGGTCGAAGAGGATGTTGAATCCGATGACTTGCCCTCAGTGATCACCGATTCAAACAGCAGGGTCAGACTCGTGAACTCCGCTTACAAGGAAATGATGGGGCAGCCAGAGTGCTCATGGCTGGATTCAATGGTGAGAGGGAAGAGGATCTGTGGAGAAGTGATGATCAATTTTTGTGAATCCAAAATTCCAGTGATGACTGAGAACAATGGGTTTTCTTGCTGGGTAAGGATAGATTGGGGAAGAGACGGTAAGGAGGAGTACATGCATGCCTTTTGCGATGTTACGAAACTTGCTTGCGATTCCAAGGATTATGTCTTTACGTGGAGGTTTCACACAACCACTGACCGCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

354

Amino Acids

39.52

Weight (kDa)

9.33

Isoelectric Point (pI)

56.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7950 PF25821 228 - 350 2e-10 Domain of unknown function (DUF7950)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016019)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27461
fragaria_vesca FvH4_2g32940
malus_domestica MD08G1080800.v1.1
prunus_persica Prupe.1G421700_v2.0.a1
pyrus_communis pycom08g06660
rosa_chinensis RchiOBHm_Chr6g0309781
rosa_laevigata RLG00000010536
rosa_multiflora Rmu_ssc0000373.1_g000008
rosa_roxburghii Rroxscaffold_7G00159190
rosa_rugosa Rorug06G0374500
rosa_samantha Rh6AG488000 Rh6BG497600 Rh6CG502400 Rh6DG488600
rosa_wichuraiana Rw6G042500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 199, 518
AciI CCGC 9 cut(s) 214, 231, 279, 516, 518, 582, 608, 789, 1057
AclWI GGATC 2 cut(s) 119, 863
AcsI RAATTY 1 cut(s) 894
AcyI GRCGYC 1 cut(s) 104
AdeI CACNNNGTG 1 cut(s) 314
AfaI GTAC 2 cut(s) 292, 971
AfiI CCNNNNNNNGG 2 cut(s) 98, 386
AflIII ACRYGT 1 cut(s) 313
AgsI TTSAA 4 cut(s) 194, 728, 762, 837
AluBI AGCT 2 cut(s) 59, 190
AluI AGCT 2 cut(s) 59, 190
Alw21I GWGCWC 1 cut(s) 824
Alw26I GTCTC 6 cut(s) 147, 256, 415, 531, 687, 948
AlwI GGATC 2 cut(s) 119, 863
Ama87I CYCGRG 2 cut(s) 98, 108
AoxI GGCC 3 cut(s) 182, 223, 620
ApeKI GCWGC 2 cut(s) 610, 811
ApoI RAATTY 1 cut(s) 894
AspLEI GCGC 1 cut(s) 199
AspS9I GGNCC 3 cut(s) 239, 323, 560
AsuC2I CCSGG 2 cut(s) 99, 100
AsuHPI GGTGA 6 cut(s) 436, 559, 597, 693, 745, 853
AvaI CYCGRG 2 cut(s) 98, 108
AvaII GGWCC 3 cut(s) 239, 323, 560
BauI CACGAG 1 cut(s) 779
Bbv12I GWGCWC 1 cut(s) 824
BbvCI CCTCAGC 1 cut(s) 186
BbvI GCAGC 2 cut(s) 622, 823
BccI CCATC 4 cut(s) 229, 572, 679, 799
BclI TGATCA 2 cut(s) 750, 873
BcnI CCSGG 2 cut(s) 99, 100
BcoDI GTCTC 6 cut(s) 147, 256, 415, 531, 687, 948
BfaI CTAG 1 cut(s) 288
BglI GCCNNNNNGGC 1 cut(s) 619
BglII AGATCT 1 cut(s) 63
BisI GCNGC 2 cut(s) 611, 812
BlsI GCNGC 2 cut(s) 612, 813
BmcAI AGTACT 1 cut(s) 292
Bme1390I CCNGG 2 cut(s) 99, 100
Bme18I GGWCC 3 cut(s) 239, 323, 560
BmeT110I CYCGRG 2 cut(s) 98, 108
BmgT120I GGNCC 3 cut(s) 239, 323, 560
BmiI GGNNCC 4 cut(s) 240, 241, 474, 562
BmrFI CCNGG 2 cut(s) 99, 100
BoxI GACNNNNGTC 1 cut(s) 268
Bpu10I CCTNAGC 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 366
BpuMI CCSGG 2 cut(s) 99, 100
BsaAI YACGTR 1 cut(s) 1032
BsaHI GRCGYC 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 256
BsaJI CCNNGG 4 cut(s) 98, 226, 516, 1014
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 386
Bse118I RCCGGY 1 cut(s) 622
Bse1I ACTGG 1 cut(s) 899
Bse3DI GCAATG 1 cut(s) 84
BseDI CCNNGG 4 cut(s) 98, 226, 516, 1014
BseGI GGATG 2 cut(s) 240, 727
BseLI CCNNNNNNNGG 2 cut(s) 98, 386
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 4 cut(s) 51, 200, 759, 900
BseNI ACTGG 1 cut(s) 899
BseRI GAGGAG 2 cut(s) 441, 980
BseXI GCAGC 2 cut(s) 622, 823
BseYI CCCAGC 1 cut(s) 930
Bsh1236I CGCG 2 cut(s) 199, 518
BshFI GGCC 3 cut(s) 184, 225, 622
BsiHKAI GWGCWC 1 cut(s) 824
BsiHKCI CYCGRG 2 cut(s) 98, 108
BsiSI CCGG 2 cut(s) 99, 623
BslFI GGGAC 2 cut(s) 115, 225
BslI CCNNNNNNNGG 2 cut(s) 98, 386
BsmAI GTCTC 6 cut(s) 147, 256, 415, 531, 687, 948
BsmBI CGTCTC 2 cut(s) 687, 948
BsmFI GGGAC 2 cut(s) 115, 225
BsnI GGCC 3 cut(s) 184, 225, 622
Bso31I GGTCTC 1 cut(s) 256
BsoBI CYCGRG 2 cut(s) 98, 108
Bsp1286I GDGCHC 1 cut(s) 824
Bsp143I GATC 8 cut(s) 63, 124, 480, 502, 636, 750, 855, 873
BspACI CCGC 9 cut(s) 214, 231, 279, 516, 518, 582, 608, 789, 1057
BspANI GGCC 3 cut(s) 184, 225, 622
BspCNI CTCAG 4 cut(s) 52, 199, 758, 901
BspFNI CGCG 2 cut(s) 199, 518
BspLI GGNNCC 4 cut(s) 240, 241, 474, 562
BspPI GGATC 2 cut(s) 119, 863
BspTNI GGTCTC 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 84
BsrFI RCCGGY 1 cut(s) 622
BsrI ACTGG 1 cut(s) 899
BssAI RCCGGY 1 cut(s) 622
BssECI CCNNGG 4 cut(s) 98, 226, 516, 1014
BssMI GATC 8 cut(s) 63, 124, 480, 502, 636, 750, 855, 873
BssNI GRCGYC 1 cut(s) 104
BssSI CACGAG 1 cut(s) 779
BssT1I CCWWGG 1 cut(s) 1014
Bst2BI CACGAG 1 cut(s) 779
Bst4CI ACNGT 3 cut(s) 339, 395, 959
Bst6I CTCTTC 7 cut(s) 250, 254, 418, 441, 711, 845, 946
BstACI GRCGYC 1 cut(s) 104
BstBAI YACGTR 1 cut(s) 1032
BstC8I GCNNGC 3 cut(s) 218, 978, 1006
BstDEI CTNAG 4 cut(s) 60, 186, 745, 909
BstDSI CCRYGG 1 cut(s) 516
BstEII GGTNACC 1 cut(s) 366
BstF5I GGATG 2 cut(s) 240, 727
BstFNI CGCG 2 cut(s) 199, 518
BstHHI GCGC 1 cut(s) 199
BstKTI GATC 8 cut(s) 66, 127, 483, 505, 639, 753, 858, 876
BstMAI GTCTC 6 cut(s) 147, 256, 415, 531, 687, 948
BstMBI GATC 8 cut(s) 63, 124, 480, 502, 636, 750, 855, 873
BstMWI GCNNNNNNNGC 4 cut(s) 103, 196, 222, 619
BstNSI RCATGY 2 cut(s) 976, 980
BstPAI GACNNNNGTC 1 cut(s) 268
BstPI GGTNACC 1 cut(s) 366
BstSCI CCNGG 2 cut(s) 97, 98
BstUI CGCG 2 cut(s) 199, 518
BstV1I GCAGC 2 cut(s) 622, 823
BstX2I RGATCY 2 cut(s) 63, 855
BstXI CCANNNNNNTGG 1 cut(s) 469
BstYI RGATCY 2 cut(s) 63, 855
BsuRI GGCC 3 cut(s) 184, 225, 622
BtgI CCRYGG 1 cut(s) 516
BtgZI GCGATG 1 cut(s) 1002
BtsCI GGATG 2 cut(s) 240, 727
BtsIMutI CAGTG 4 cut(s) 335, 753, 906, 1050
Cac8I GCNNGC 3 cut(s) 218, 978, 1006
CfoI GCGC 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 622
Cfr13I GGNCC 3 cut(s) 239, 323, 560
Cfr42I CCGCGG 1 cut(s) 519
Cfr9I CCCGGG 1 cut(s) 98
CseI GACGC 2 cut(s) 112, 556
Csp6I GTAC 2 cut(s) 291, 970
CviAII CATG 5 cut(s) 276, 469, 825, 973, 977
CviQI GTAC 2 cut(s) 291, 970
DdeI CTNAG 4 cut(s) 60, 186, 745, 909
DpnI GATC 8 cut(s) 65, 126, 482, 504, 638, 752, 857, 875
DpnII GATC 8 cut(s) 63, 124, 480, 502, 636, 750, 855, 873
DraIII CACNNNGTG 1 cut(s) 314
Eam1104I CTCTTC 7 cut(s) 250, 254, 418, 441, 711, 845, 946
EarI CTCTTC 7 cut(s) 250, 254, 418, 441, 711, 845, 946
Eco130I CCWWGG 1 cut(s) 1014
Eco31I GGTCTC 1 cut(s) 256
Eco47I GGWCC 3 cut(s) 239, 323, 560
Eco88I CYCGRG 2 cut(s) 98, 108
Eco91I GGTNACC 1 cut(s) 366
EcoO109I RGGNCCY 2 cut(s) 239, 560
EcoO65I GGTNACC 1 cut(s) 366
EcoT14I CCWWGG 1 cut(s) 1014
EcoT22I ATGCAT 2 cut(s) 470, 978
ErhI CCWWGG 1 cut(s) 1014
Esp3I CGTCTC 2 cut(s) 687, 948
FaeI CATG 5 cut(s) 279, 472, 828, 976, 980
FaqI GGGAC 2 cut(s) 115, 225
FatI CATG 5 cut(s) 275, 468, 824, 972, 976
FauI CCCGC 2 cut(s) 221, 224
FbaI TGATCA 2 cut(s) 750, 873
Fnu4HI GCNGC 2 cut(s) 611, 812
FokI GGATG 2 cut(s) 247, 734
Fsp4HI GCNGC 2 cut(s) 611, 812
FspBI CTAG 1 cut(s) 288
GlaI GCGC 1 cut(s) 198
GluI GCNGC 2 cut(s) 611, 812
GsaI CCCAGC 1 cut(s) 934
HaeIII GGCC 3 cut(s) 184, 225, 622
HapII CCGG 2 cut(s) 99, 623
HgaI GACGC 2 cut(s) 112, 556
HhaI GCGC 1 cut(s) 199
Hin1I GRCGYC 1 cut(s) 104
Hin1II CATG 5 cut(s) 279, 472, 828, 976, 980
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HinfI GANTC 7 cut(s) 597, 728, 758, 777, 833, 887, 1010
HpaII CCGG 2 cut(s) 99, 623
HphI GGTGA 6 cut(s) 436, 559, 597, 693, 745, 853
Hpy166II GTNNAC 2 cut(s) 115, 784
Hpy188I TCNGA 3 cut(s) 507, 733, 776
Hpy188III TCNNGA 2 cut(s) 544, 781
Hpy8I GTNNAC 2 cut(s) 115, 784
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 3 cut(s) 138, 179, 465
HpyCH4III ACNGT 3 cut(s) 339, 395, 959
HpyCH4IV ACGT 4 cut(s) 313, 642, 680, 1031
HpyCH4V TGCA 3 cut(s) 89, 468, 976
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 196, 222, 619
HpyF3I CTNAG 4 cut(s) 60, 186, 745, 909
HpySE526I ACGT 4 cut(s) 313, 642, 680, 1031
Hsp92I GRCGYC 1 cut(s) 104
Hsp92II CATG 5 cut(s) 279, 472, 828, 976, 980
HspAI GCGC 1 cut(s) 197
KflI GGGWCCC 1 cut(s) 239
Ksp22I TGATCA 2 cut(s) 750, 873
KspI CCGCGG 1 cut(s) 519
Kzo9I GATC 8 cut(s) 63, 124, 480, 502, 636, 750, 855, 873
LmnI GCTCC 1 cut(s) 472
Lsp1109I GCAGC 2 cut(s) 622, 823
MaeI CTAG 1 cut(s) 288
MaeII ACGT 4 cut(s) 313, 642, 680, 1031
MaeIII GTNAC 4 cut(s) 173, 366, 424, 991
MalI GATC 8 cut(s) 65, 126, 482, 504, 638, 752, 857, 875
MboI GATC 8 cut(s) 63, 124, 480, 502, 636, 750, 855, 873
MboII GAAGA 9 cut(s) 123, 237, 271, 276, 405, 428, 728, 862, 963
MflI RGATCY 2 cut(s) 63, 855
MhlI GDGCHC 1 cut(s) 824
MluCI AATT 2 cut(s) 877, 894
MlyI GAGTC 2 cut(s) 606, 771
MmeI TCCRAC 1 cut(s) 701
Mph1103I ATGCAT 2 cut(s) 470, 978
MseI TTAA 2 cut(s) 51, 330
MspA1I CMGCKG 2 cut(s) 59, 518
MspI CCGG 2 cut(s) 99, 623
MspR9I CCNGG 2 cut(s) 99, 100
MvnI CGCG 2 cut(s) 199, 518
MwoI GCNNNNNNNGC 4 cut(s) 103, 196, 222, 619
NciI CCSGG 2 cut(s) 99, 100
NdeII GATC 8 cut(s) 63, 124, 480, 502, 636, 750, 855, 873
NlaIII CATG 5 cut(s) 279, 472, 828, 976, 980
NlaIV GGNNCC 4 cut(s) 240, 241, 474, 562
NmeAIII GCCGAG 1 cut(s) 251
NmuCI GTSAC 1 cut(s) 424
NsiI ATGCAT 2 cut(s) 470, 978
NspI RCATGY 2 cut(s) 976, 980
PaeI GCATGC 1 cut(s) 980
PaeR7I CTCGAG 1 cut(s) 108
PfeI GAWTC 5 cut(s) 728, 758, 833, 887, 1010
PkrI GCNGC 2 cut(s) 612, 813
PleI GAGTC 2 cut(s) 605, 771
PpsI GAGTC 2 cut(s) 605, 771
Ppu21I YACGTR 1 cut(s) 1032
PpuMI RGGWCCY 2 cut(s) 239, 560
PshAI GACNNNNGTC 1 cut(s) 268
Psp5II RGGWCCY 2 cut(s) 239, 560
PspEI GGTNACC 1 cut(s) 366
PspFI CCCAGC 1 cut(s) 930
PspN4I GGNNCC 4 cut(s) 240, 241, 474, 562
PspPI GGNCC 3 cut(s) 239, 323, 560
PspPPI RGGWCCY 2 cut(s) 239, 560
PspXI VCTCGAGB 1 cut(s) 108
PsrI GAACNNNNNNTAC 2 cut(s) 776, 808
PsuI RGATCY 2 cut(s) 63, 855
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 2 cut(s) 292, 971
RsaNI GTAC 2 cut(s) 291, 970
SacII CCGCGG 1 cut(s) 519
SaqAI TTAA 2 cut(s) 51, 330
SatI GCNGC 2 cut(s) 611, 812
Sau3AI GATC 8 cut(s) 63, 124, 480, 502, 636, 750, 855, 873
Sau96I GGNCC 3 cut(s) 239, 323, 560
ScaI AGTACT 1 cut(s) 292
SchI GAGTC 2 cut(s) 606, 771
ScrFI CCNGG 2 cut(s) 99, 100
SduI GDGCHC 1 cut(s) 824
Sfr274I CTCGAG 1 cut(s) 108
Sfr303I CCGCGG 1 cut(s) 519
SgrBI CCGCGG 1 cut(s) 519
SinI GGWCC 3 cut(s) 239, 323, 560
SlaI CTCGAG 1 cut(s) 108
SmaI CCCGGG 1 cut(s) 100
SmlI CTYRAG 2 cut(s) 108, 381
SmoI CTYRAG 2 cut(s) 108, 381
SphI GCATGC 1 cut(s) 980
Sse9I AATT 2 cut(s) 877, 894
SsiI CCGC 9 cut(s) 214, 231, 279, 516, 518, 582, 608, 789, 1057
SspMI CTAG 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 97, 98
StyI CCWWGG 1 cut(s) 1014
TaaI ACNGT 3 cut(s) 339, 395, 959
TaiI ACGT 4 cut(s) 316, 645, 683, 1034
TaqI TCGA 2 cut(s) 109, 714
TasI AATT 2 cut(s) 877, 894
TatI WGTACW 2 cut(s) 290, 969
TfiI GAWTC 5 cut(s) 728, 758, 833, 887, 1010
Tru1I TTAA 2 cut(s) 51, 330
Tru9I TTAA 2 cut(s) 51, 330
TscAI CASTG 4 cut(s) 342, 753, 906, 1057
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 2 cut(s) 610, 811
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 1 cut(s) 272
TspMI CCCGGG 1 cut(s) 98
TspRI CASTG 4 cut(s) 342, 753, 906, 1057
VpaK11BI GGWCC 3 cut(s) 239, 323, 560
XapI RAATTY 1 cut(s) 894
XceI RCATGY 2 cut(s) 976, 980
XcmI CCANNNNNNNNNTGG 1 cut(s) 822
XhoI CTCGAG 1 cut(s) 108
XmaI CCCGGG 1 cut(s) 98
XspI CTAG 1 cut(s) 288
ZrmI AGTACT 1 cut(s) 292
Zsp2I ATGCAT 2 cut(s) 470, 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.