MD08G1080800.v1.1

maturation of SSU-rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016019
Physical Location & Seq
Forward (+)
6728556 .. 6729705
1150 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1080800.v1.1.491

Sequence Viewer

Length: 1110 bp
ATGATCAAGACGTTGAATCCCTACACCAACACGGAGAAAACCGCTGAGATTATGTCCAGGTACAGGCCCATAGCGCCAAAACCTGTGACCCCATCAAACCCAGCAGGCGAAAGCCCGTCCATGTCTCAGAAAATCAGGGACTCTCCTTACCTTAGGAACCTCTGGCCGCAGTTGCAGGCCAGACCCACCAGGACTCGAAAGCGGGGTCGATCAGCTCTCTCTCCGACCACCTTTAAGAGGCAGAGGAGTCATCAAATGCTAGGCTTTGCCACTCCTTGTCATGTGCAATCTCCGGTGAAAAATCCGAGATTGGATGGTTTTGCTCGTGTGCTTCCGAAGCTTCCCATTCCGGCCAGCCTCGATGCCGCCAACAACTCCACCGGTTTGATGACTCTGCCTCTTCTGCCATATATCCCTCCTGCGTCGGCCCCCATTGTCACAAACCCAGCGGTGGTGCCGCCAGAGTTCGAATTGATCAAACCCTGCCGAGGAGGAGAGGAGGAGCTGATTGATTTGAACACCGTGGCAGCTGAAATCCCAGAGGAAAAAGATCTGCTCAAGCAACTACAGGGGAATTCCCCTGCACCTCCACCAACACCCACCAATGTCATAGCGCCGCAGCCAATTCGACCGGTGGGTTCCAGCATAAGCGTTGGATGCATCAGCGCAGACCCGAGCTTAACTCTGGCAGCGCAAGTTCCTAAGAAACCAGAGGACGTCGAGGAGGAGATGGAGTCTGAAGCCCTGCCAGCAATCATATCAGACTCACACAACAAAGTGAGGATGGCAAACTCTGCCTACAAGGAAATGGTGGGCCAGCCAGAGTGTCCATGGCTCGACGCCATGGTGGCCGGAGGCGACGGGCGGTTTGGAGGCAAGAGGATCAACGGGGAGGTCAGTCTGCAGCTTTCTGATTCCGGAGTGCCCGTTTCCTCAAACGGGTTCTCCTGCTGGGTGAGGATTGAGTGGGGGAAAGACATGAACAAGCACGCGATCAACACGTTCTGCGACGTGATGAAGCTGTCCTGTGAGTCCAAGGACTACCTCTTTGAATGGAGGTTCCACACCCACAGCAGAGAGGGCTGTCAGTCCTCCAGCTCCAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

370

Amino Acids

40.31

Weight (kDa)

8.36

Isoelectric Point (pI)

64.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7950 PF25821 237 - 355 9.9e-12 Domain of unknown function (DUF7950)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016019)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27461
fragaria_vesca FvH4_2g32940
malus_domestica MD08G1080800.v1.1
prunus_persica Prupe.1G421700_v2.0.a1
pyrus_communis pycom08g06660
rosa_chinensis RchiOBHm_Chr6g0309781
rosa_laevigata RLG00000010536
rosa_multiflora Rmu_ssc0000373.1_g000008
rosa_roxburghii Rroxscaffold_7G00159190
rosa_rugosa Rorug06G0374500
rosa_samantha Rh6AG488000 Rh6BG497600 Rh6CG502400 Rh6DG488600
rosa_wichuraiana Rw6G042500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 720
AccB1I GGYRCC 1 cut(s) 454
AccII CGCG 1 cut(s) 992
AccIII TCCGGA 1 cut(s) 917
AciI CCGC 8 cut(s) 42, 167, 202, 366, 449, 458, 617, 865
AclWI GGATC 1 cut(s) 890
AcoI YGGCCR 3 cut(s) 164, 351, 849
AcsI RAATTY 1 cut(s) 574
AcuI CTGAAG 1 cut(s) 759
AcyI GRCGYC 2 cut(s) 717, 840
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 5 cut(s) 63, 237, 451, 488, 939
AflIII ACRYGT 1 cut(s) 999
AgeI ACCGGT 2 cut(s) 380, 631
AgsI TTSAA 3 cut(s) 16, 517, 1052
AjiI CACGTC 1 cut(s) 1012
AjnI CCWGG 2 cut(s) 56, 188
AluBI AGCT 8 cut(s) 215, 340, 505, 530, 678, 907, 1021, 1098
AluI AGCT 8 cut(s) 215, 340, 505, 530, 678, 907, 1021, 1098
Alw26I GTCTC 1 cut(s) 129
AlwI GGATC 1 cut(s) 890
Ama87I CYCGRG 1 cut(s) 673
Aor13HI TCCGGA 1 cut(s) 917
AoxI GGCC 7 cut(s) 65, 164, 177, 351, 426, 814, 849
ApeKI GCWGC 4 cut(s) 527, 619, 689, 904
ApoI RAATTY 1 cut(s) 574
ArsI GACNNNNNNTTYG 6 cut(s) 190, 222, 851, 883, 1031, 1063
AsiGI ACCGGT 2 cut(s) 380, 631
AspLEI GCGC 4 cut(s) 76, 616, 668, 694
AspS9I GGNCC 3 cut(s) 66, 427, 814
AsuHPI GGTGA 2 cut(s) 307, 967
AsuII TTCGAA 1 cut(s) 468
AvaI CYCGRG 1 cut(s) 673
AxyI CCTNAGG 1 cut(s) 152
BaeGI GKGCMC 1 cut(s) 927
BanI GGYRCC 1 cut(s) 454
BauI CACGAG 1 cut(s) 324
BbvI GCAGC 4 cut(s) 539, 631, 701, 916
BccI CCATC 4 cut(s) 100, 308, 724, 778
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BciT130I CCWGG 2 cut(s) 58, 190
BclI TGATCA 2 cut(s) 3, 474
BcoDI GTCTC 1 cut(s) 129
BfaI CTAG 1 cut(s) 260
BfmI CTRYAG 2 cut(s) 566, 902
BfoI RGCGCY 2 cut(s) 77, 617
BglI GCCNNNNNGGC 1 cut(s) 848
BglII AGATCT 1 cut(s) 550
BisI GCNGC 8 cut(s) 167, 366, 458, 528, 617, 620, 690, 905
BlsI GCNGC 8 cut(s) 168, 367, 459, 529, 618, 621, 691, 906
Bme1390I CCNGG 2 cut(s) 58, 190
BmeT110I CYCGRG 1 cut(s) 673
BmgBI CACGTC 1 cut(s) 1012
BmgT120I GGNCC 3 cut(s) 66, 427, 814
BmiI GGNNCC 5 cut(s) 158, 429, 456, 640, 1061
BmrFI CCNGG 2 cut(s) 58, 190
BmsI GCATC 3 cut(s) 352, 647, 669
BplI GAGNNNNNCTC 2 cut(s) 667, 699
BpmI CTGGAG 2 cut(s) 1078, 1084
Bpu14I TTCGAA 1 cut(s) 468
BpuEI CTTGAG 1 cut(s) 542
BsaHI GRCGYC 2 cut(s) 717, 840
BsaJI CCNNGG 5 cut(s) 487, 522, 830, 843, 1035
BsaWI WCCGGW 4 cut(s) 292, 380, 631, 917
BsaXI ACNNNNNCTCC 6 cut(s) 719, 749, 912, 929, 942, 959
Bsc4I CCNNNNNNNGG 5 cut(s) 63, 237, 451, 488, 939
Bse118I RCCGGY 2 cut(s) 380, 631
Bse1I ACTGG 1 cut(s) 1101
Bse21I CCTNAGG 1 cut(s) 152
BseAI TCCGGA 1 cut(s) 917
BseBI CCWGG 2 cut(s) 58, 190
BseDI CCNNGG 5 cut(s) 487, 522, 830, 843, 1035
BseGI GGATG 3 cut(s) 319, 662, 789
BseLI CCNNNNNNNGG 5 cut(s) 63, 237, 451, 488, 939
BseMII CTCAG 2 cut(s) 36, 140
BseNI ACTGG 1 cut(s) 1101
BseRI GAGGAG 7 cut(s) 259, 504, 507, 512, 515, 737, 740
BseSI GKGCMC 1 cut(s) 927
BseXI GCAGC 4 cut(s) 539, 631, 701, 916
BseYI CCCAGC 3 cut(s) 100, 445, 951
BsgI GTGCAG 1 cut(s) 567
Bsh1236I CGCG 1 cut(s) 992
Bsh1285I CGRYCG 1 cut(s) 632
BshFI GGCC 7 cut(s) 67, 166, 179, 353, 428, 816, 851
BshNI GGYRCC 1 cut(s) 454
BshTI ACCGGT 2 cut(s) 380, 631
BsiEI CGRYCG 1 cut(s) 632
BsiHKCI CYCGRG 1 cut(s) 673
BsiSI CCGG 6 cut(s) 293, 350, 381, 632, 852, 918
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 5 cut(s) 63, 237, 451, 488, 939
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 7 cut(s) 67, 166, 179, 353, 428, 816, 851
BsoBI CYCGRG 1 cut(s) 673
Bsp119I TTCGAA 1 cut(s) 468
Bsp1286I GDGCHC 1 cut(s) 927
Bsp13I TCCGGA 1 cut(s) 917
Bsp143I GATC 6 cut(s) 3, 209, 474, 550, 882, 993
Bsp19I CCATGG 2 cut(s) 830, 843
BspACI CCGC 8 cut(s) 42, 167, 202, 366, 449, 458, 617, 865
BspANI GGCC 7 cut(s) 67, 166, 179, 353, 428, 816, 851
BspCNI CTCAG 2 cut(s) 37, 139
BspEI TCCGGA 1 cut(s) 917
BspFNI CGCG 1 cut(s) 992
BspLI GGNNCC 5 cut(s) 158, 429, 456, 640, 1061
BspMAI CTGCAG 1 cut(s) 906
BspPI GGATC 1 cut(s) 890
BspT104I TTCGAA 1 cut(s) 468
BspT107I GGYRCC 1 cut(s) 454
BsrFI RCCGGY 2 cut(s) 380, 631
BsrI ACTGG 1 cut(s) 1101
BssAI RCCGGY 2 cut(s) 380, 631
BssECI CCNNGG 5 cut(s) 487, 522, 830, 843, 1035
BssMI GATC 6 cut(s) 3, 209, 474, 550, 882, 993
BssNI GRCGYC 2 cut(s) 717, 840
BssSI CACGAG 1 cut(s) 324
BssT1I CCWWGG 3 cut(s) 830, 843, 1035
Bst2BI CACGAG 1 cut(s) 324
Bst2UI CCWGG 2 cut(s) 58, 190
Bst4CI ACNGT 1 cut(s) 523
Bst6I CTCTTC 1 cut(s) 405
BstACI GRCGYC 2 cut(s) 717, 840
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBI TTCGAA 1 cut(s) 468
BstC8I GCNNGC 6 cut(s) 106, 177, 355, 750, 818, 990
BstDEI CTNAG 4 cut(s) 45, 126, 152, 702
BstDSI CCRYGG 3 cut(s) 522, 830, 843
BstENI CCTNNNNNAGG 1 cut(s) 235
BstF5I GGATG 3 cut(s) 319, 662, 789
BstFNI CGCG 1 cut(s) 992
BstH2I RGCGCY 2 cut(s) 77, 617
BstHHI GCGC 4 cut(s) 76, 616, 668, 694
BstKTI GATC 6 cut(s) 6, 212, 477, 553, 885, 996
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 6 cut(s) 3, 209, 474, 550, 882, 993
BstMCI CGRYCG 1 cut(s) 632
BstMWI GCNNNNNNNGC 9 cut(s) 73, 172, 337, 403, 657, 749, 794, 848, 1080
BstNI CCWGG 2 cut(s) 58, 190
BstSCI CCNGG 2 cut(s) 56, 188
BstSFI CTRYAG 2 cut(s) 566, 902
BstSLI GKGCMC 1 cut(s) 927
BstUI CGCG 1 cut(s) 992
BstV1I GCAGC 4 cut(s) 539, 631, 701, 916
BstX2I RGATCY 1 cut(s) 550
BstYI RGATCY 1 cut(s) 550
Bsu36I CCTNAGG 1 cut(s) 152
BsuRI GGCC 7 cut(s) 67, 166, 179, 353, 428, 816, 851
BtgI CCRYGG 3 cut(s) 522, 830, 843
BtrI CACGTC 1 cut(s) 1012
BtsCI GGATG 3 cut(s) 319, 662, 789
BtsIMutI CAGTG 1 cut(s) 1108
Cac8I GCNNGC 6 cut(s) 106, 177, 355, 750, 818, 990
CfoI GCGC 4 cut(s) 76, 616, 668, 694
Cfr10I RCCGGY 2 cut(s) 380, 631
Cfr13I GGNCC 3 cut(s) 66, 427, 814
CseI GACGC 2 cut(s) 411, 848
Csp6I GTAC 1 cut(s) 61
CspAI ACCGGT 2 cut(s) 380, 631
CviAII CATG 5 cut(s) 121, 281, 831, 844, 979
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 4 cut(s) 45, 126, 152, 702
DpnI GATC 6 cut(s) 5, 211, 476, 552, 884, 995
DpnII GATC 6 cut(s) 3, 209, 474, 550, 882, 993
EaeI YGGCCR 3 cut(s) 164, 351, 849
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Eco130I CCWWGG 3 cut(s) 830, 843, 1035
Eco57I CTGAAG 1 cut(s) 759
Eco81I CCTNAGG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 673
EcoNI CCTNNNNNAGG 1 cut(s) 235
EcoRI GAATTC 1 cut(s) 574
EcoRII CCWGG 2 cut(s) 56, 188
EcoT14I CCWWGG 3 cut(s) 830, 843, 1035
EcoT22I ATGCAT 1 cut(s) 662
ErhI CCWWGG 3 cut(s) 830, 843, 1035
FaeI CATG 5 cut(s) 124, 284, 834, 847, 982
FaqI GGGAC 1 cut(s) 152
FatI CATG 5 cut(s) 120, 280, 830, 843, 978
FauI CCCGC 1 cut(s) 195
FbaI TGATCA 2 cut(s) 3, 474
Fnu4HI GCNGC 8 cut(s) 167, 366, 458, 528, 617, 620, 690, 905
FokI GGATG 3 cut(s) 326, 669, 796
Fsp4HI GCNGC 8 cut(s) 167, 366, 458, 528, 617, 620, 690, 905
FspBI CTAG 1 cut(s) 260
GlaI GCGC 4 cut(s) 75, 615, 667, 693
GluI GCNGC 8 cut(s) 167, 366, 458, 528, 617, 620, 690, 905
GsaI CCCAGC 3 cut(s) 104, 449, 955
GsuI CTGGAG 2 cut(s) 1078, 1084
HaeII RGCGCY 2 cut(s) 77, 617
HaeIII GGCC 7 cut(s) 67, 166, 179, 353, 428, 816, 851
HapII CCGG 6 cut(s) 293, 350, 381, 632, 852, 918
HgaI GACGC 2 cut(s) 411, 848
HhaI GCGC 4 cut(s) 76, 616, 668, 694
Hin1I GRCGYC 2 cut(s) 717, 840
Hin1II CATG 5 cut(s) 124, 284, 834, 847, 982
Hin6I GCGC 4 cut(s) 74, 614, 666, 692
HinP1I GCGC 4 cut(s) 74, 614, 666, 692
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 9 cut(s) 16, 140, 193, 247, 391, 734, 764, 914, 1031
HpaII CCGG 6 cut(s) 293, 350, 381, 632, 852, 918
HphI GGTGA 2 cut(s) 307, 967
Hpy188I TCNGA 7 cut(s) 129, 225, 306, 336, 739, 763, 913
Hpy188III TCNNGA 2 cut(s) 7, 918
Hpy99I CGWCG 5 cut(s) 427, 722, 842, 863, 1013
HpyCH4III ACNGT 1 cut(s) 523
HpyCH4IV ACGT 4 cut(s) 11, 717, 1001, 1011
HpyCH4V TGCA 5 cut(s) 175, 286, 584, 660, 904
HpyF10VI GCNNNNNNNGC 9 cut(s) 73, 172, 337, 403, 657, 749, 794, 848, 1080
HpyF3I CTNAG 4 cut(s) 45, 126, 152, 702
HpySE526I ACGT 4 cut(s) 11, 717, 1001, 1011
Hsp92I GRCGYC 2 cut(s) 717, 840
Hsp92II CATG 5 cut(s) 124, 284, 834, 847, 982
HspAI GCGC 4 cut(s) 74, 614, 666, 692
Kpn2I TCCGGA 1 cut(s) 917
Ksp22I TGATCA 2 cut(s) 3, 474
Kzo9I GATC 6 cut(s) 3, 209, 474, 550, 882, 993
LmnI GCTCC 2 cut(s) 502, 1103
Lsp1109I GCAGC 4 cut(s) 539, 631, 701, 916
LweI GCATC 3 cut(s) 352, 647, 669
MaeI CTAG 1 cut(s) 260
MaeII ACGT 4 cut(s) 11, 717, 1001, 1011
MaeIII GTNAC 2 cut(s) 85, 436
MalI GATC 6 cut(s) 5, 211, 476, 552, 884, 995
MboI GATC 6 cut(s) 3, 209, 474, 550, 882, 993
MboII GAAGA 1 cut(s) 392
MflI RGATCY 1 cut(s) 550
MhlI GDGCHC 1 cut(s) 927
MluCI AATT 3 cut(s) 470, 574, 624
MlyI GAGTC 7 cut(s) 134, 187, 256, 385, 743, 758, 1040
MmeI TCCRAC 2 cut(s) 248, 634
Mph1103I ATGCAT 1 cut(s) 662
MroI TCCGGA 1 cut(s) 917
MseI TTAA 2 cut(s) 234, 680
MspA1I CMGCKG 3 cut(s) 44, 449, 530
MspI CCGG 6 cut(s) 293, 350, 381, 632, 852, 918
MspR9I CCNGG 2 cut(s) 58, 190
MvaI CCWGG 2 cut(s) 58, 190
MvnI CGCG 1 cut(s) 992
MwoI GCNNNNNNNGC 9 cut(s) 73, 172, 337, 403, 657, 749, 794, 848, 1080
NcoI CCATGG 2 cut(s) 830, 843
NdeII GATC 6 cut(s) 3, 209, 474, 550, 882, 993
NlaIII CATG 5 cut(s) 124, 284, 834, 847, 982
NlaIV GGNNCC 5 cut(s) 158, 429, 456, 640, 1061
NmeAIII GCCGAG 1 cut(s) 512
NmuCI GTSAC 2 cut(s) 85, 436
NsiI ATGCAT 1 cut(s) 662
NspV TTCGAA 1 cut(s) 468
PfeI GAWTC 2 cut(s) 16, 914
PinAI ACCGGT 2 cut(s) 380, 631
PkrI GCNGC 8 cut(s) 168, 367, 459, 529, 618, 621, 691, 906
PleI GAGTC 7 cut(s) 134, 187, 255, 385, 742, 758, 1039
PpsI GAGTC 7 cut(s) 134, 187, 255, 385, 742, 758, 1039
Psp6I CCWGG 2 cut(s) 56, 188
PspFI CCCAGC 3 cut(s) 100, 445, 951
PspGI CCWGG 2 cut(s) 56, 188
PspN4I GGNNCC 5 cut(s) 158, 429, 456, 640, 1061
PspPI GGNCC 3 cut(s) 66, 427, 814
PstI CTGCAG 1 cut(s) 906
PsuI RGATCY 1 cut(s) 550
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SaqAI TTAA 2 cut(s) 234, 680
SatI GCNGC 8 cut(s) 167, 366, 458, 528, 617, 620, 690, 905
Sau3AI GATC 6 cut(s) 3, 209, 474, 550, 882, 993
Sau96I GGNCC 3 cut(s) 66, 427, 814
SchI GAGTC 7 cut(s) 134, 187, 256, 385, 743, 758, 1040
ScrFI CCNGG 2 cut(s) 58, 190
SduI GDGCHC 1 cut(s) 927
SfaNI GCATC 3 cut(s) 352, 647, 669
SfcI CTRYAG 2 cut(s) 566, 902
SfuI TTCGAA 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
Sse9I AATT 3 cut(s) 470, 574, 624
SsiI CCGC 8 cut(s) 42, 167, 202, 366, 449, 458, 617, 865
SspMI CTAG 1 cut(s) 260
StyD4I CCNGG 2 cut(s) 56, 188
StyI CCWWGG 3 cut(s) 830, 843, 1035
TaaI ACNGT 1 cut(s) 523
TaiI ACGT 4 cut(s) 14, 720, 1004, 1014
TaqI TCGA 7 cut(s) 196, 208, 360, 468, 628, 720, 837
TasI AATT 3 cut(s) 470, 574, 624
TauI GCSGC 4 cut(s) 169, 368, 460, 619
TfiI GAWTC 2 cut(s) 16, 914
Tru1I TTAA 2 cut(s) 234, 680
Tru9I TTAA 2 cut(s) 234, 680
TscAI CASTG 1 cut(s) 1108
TseFI GTSAC 2 cut(s) 85, 436
TseI GCWGC 4 cut(s) 527, 619, 689, 904
Tsp45I GTSAC 2 cut(s) 85, 436
TspDTI ATGAA 2 cut(s) 995, 1031
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 1 cut(s) 1108
XagI CCTNNNNNAGG 1 cut(s) 235
XapI RAATTY 1 cut(s) 574
XcmI CCANNNNNNNNNTGG 1 cut(s) 828
XspI CTAG 1 cut(s) 260
ZraI GACGTC 1 cut(s) 718
Zsp2I ATGCAT 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.