pycom08g06660

maturation of SSU-rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0016019
Physical Location & Seq
Forward (+)
5211098 .. 5212201
1104 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g06660.1

Sequence Viewer

Length: 1104 bp
ATGATCAAGGCATTGAACCCCTACACCAACACGGAGAAAACCGCTGAGATTATGTCCAGGTACAGGCCCATAGCGCCAAAACCTGTGACCCCATCAAACCCAGCAGGCGAAAGCCCGTCCATGTCTCAGAAAATCAGGGATTCTCCTTACCTTAGGAACCTCTGGCCGCAGTTGCAGGCCAGACCCACCAGGACTCGAAAGCGGGGTCGATCAGCTCTCTCTCCGACCACCTTTAAGAGGCCGAGGAGTCATCACATGCTAGGCTTTTCCACTCCTTGTCATGTGCAATCTCCGGTGAAAAATCCGAGATTGGACGGTTTTGCTCGTGTGCTTCCGAAGCTTCCCATTCCGACCAGCCTCGATGCCGCCAACAACTCCACCGGTTTGATGACTCTGCCTCTTCTGCCACATATCCCTCCTGCGTCGGTCCCCATTGTCACAAACCCAGCAGTGGTGCCGCCAGAGTTCGAATTGATCAAACCCTGCGGAGGAGAGGAGCTGATTGATTTGAACACCGTGGCAGCTGAAATCCCAGAGGAAAAAGATCTGCTCAAGCAACTACAGGAGAATTCCCCCGCACCTCCACCAACATCCACCAATGTCATAGCGCCGCAGCCAATTCGACCGGTGGGTTCCAGCATAAGCGTTGGATGCATCAGCGCAGACCCGAGCTTAACTCTGGCAGCGCAAGTTCCTAAGAAACCAGAGGACGTCGAGGAGGAGATGGAGTCTGAAGCCCTGCCAGCAATCATATCAGACTCGCACCACAAAGTGAGGATGGCGAACTCTGCCTACAAGGAAATGGTGGGCCAGCCAGAGTGTCCATGGCTCGACGCCATGGTGGCCGGAGGCGACGGGCGGTTTGGAGGCAAGAGGATCAGCGGGGAGGTCAGTCTGCAGCTTTCTGATTCCGGAGTGCCCGTTTCCTCAAACGGGTTCTCCTGCTGGGTGAGGATTGAGTGGGGGAAAGACATGAACAAGCACGCGATCAACACGTTCTGCGACGTGATGAAGCTGTCCTGTGAGTCCAAGGACTACCTCTTTGAATGGAGGTTCCACACCCGCAGCAGAGAGGGTTGTCAGTCCTCCAGCTCCAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

368

Amino Acids

40.1

Weight (kDa)

8.37

Isoelectric Point (pI)

66.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7950 PF25821 235 - 353 5.4e-11 Domain of unknown function (DUF7950)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016019)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27461
fragaria_vesca FvH4_2g32940
malus_domestica MD08G1080800.v1.1
prunus_persica Prupe.1G421700_v2.0.a1
pyrus_communis pycom08g06660
rosa_chinensis RchiOBHm_Chr6g0309781
rosa_laevigata RLG00000010536
rosa_multiflora Rmu_ssc0000373.1_g000008
rosa_roxburghii Rroxscaffold_7G00159190
rosa_rugosa Rorug06G0374500
rosa_samantha Rh6AG488000 Rh6BG497600 Rh6CG502400 Rh6DG488600
rosa_wichuraiana Rw6G042500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 714
AccB1I GGYRCC 1 cut(s) 454
AccII CGCG 1 cut(s) 986
AccIII TCCGGA 1 cut(s) 911
AclWI GGATC 1 cut(s) 884
AcoI YGGCCR 2 cut(s) 164, 843
AcsI RAATTY 1 cut(s) 568
AcuI CTGAAG 1 cut(s) 753
AcyI GRCGYC 2 cut(s) 711, 834
AdeI CACNNNGTG 1 cut(s) 772
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 5 cut(s) 63, 237, 451, 488, 933
AflIII ACRYGT 1 cut(s) 993
AgeI ACCGGT 2 cut(s) 380, 625
AgsI TTSAA 3 cut(s) 16, 511, 1046
AjiI CACGTC 1 cut(s) 1006
AjnI CCWGG 2 cut(s) 56, 188
AluBI AGCT 8 cut(s) 215, 340, 499, 524, 672, 901, 1015, 1092
AluI AGCT 8 cut(s) 215, 340, 499, 524, 672, 901, 1015, 1092
Alw26I GTCTC 1 cut(s) 129
AlwI GGATC 1 cut(s) 884
Ama87I CYCGRG 1 cut(s) 667
Aor13HI TCCGGA 1 cut(s) 911
AoxI GGCC 6 cut(s) 65, 164, 177, 239, 808, 843
ApeKI GCWGC 5 cut(s) 521, 613, 683, 898, 1065
ApoI RAATTY 1 cut(s) 568
ArsI GACNNNNNNTTYG 6 cut(s) 190, 222, 845, 877, 1025, 1057
AsiGI ACCGGT 2 cut(s) 380, 625
AspLEI GCGC 4 cut(s) 76, 610, 662, 688
AspS9I GGNCC 3 cut(s) 66, 427, 808
AsuHPI GGTGA 2 cut(s) 307, 961
AsuII TTCGAA 1 cut(s) 468
AvaI CYCGRG 1 cut(s) 667
AvaII GGWCC 1 cut(s) 427
AxyI CCTNAGG 1 cut(s) 152
BaeGI GKGCMC 1 cut(s) 921
BanI GGYRCC 1 cut(s) 454
BauI CACGAG 1 cut(s) 324
BbvI GCAGC 5 cut(s) 533, 625, 695, 910, 1077
BccI CCATC 3 cut(s) 100, 718, 772
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciT130I CCWGG 2 cut(s) 58, 190
BclI TGATCA 2 cut(s) 3, 474
BcoDI GTCTC 1 cut(s) 129
BfaI CTAG 1 cut(s) 260
BfmI CTRYAG 2 cut(s) 560, 896
BfoI RGCGCY 2 cut(s) 77, 611
BglI GCCNNNNNGGC 1 cut(s) 842
BglII AGATCT 1 cut(s) 544
BisI GCNGC 9 cut(s) 167, 366, 458, 522, 611, 614, 684, 899, 1066
BlsI GCNGC 9 cut(s) 168, 367, 459, 523, 612, 615, 685, 900, 1067
Bme1390I CCNGG 2 cut(s) 58, 190
Bme18I GGWCC 1 cut(s) 427
BmeT110I CYCGRG 1 cut(s) 667
BmgBI CACGTC 1 cut(s) 1006
BmgT120I GGNCC 3 cut(s) 66, 427, 808
BmiI GGNNCC 5 cut(s) 158, 429, 456, 634, 1055
BmrFI CCNGG 2 cut(s) 58, 190
BmsI GCATC 3 cut(s) 352, 641, 663
BplI GAGNNNNNCTC 2 cut(s) 661, 693
BpmI CTGGAG 2 cut(s) 1072, 1078
Bpu14I TTCGAA 1 cut(s) 468
BpuEI CTTGAG 1 cut(s) 536
BsaHI GRCGYC 2 cut(s) 711, 834
BsaJI CCNNGG 5 cut(s) 242, 516, 824, 837, 1029
BsaWI WCCGGW 4 cut(s) 292, 380, 625, 911
BsaXI ACNNNNNCTCC 6 cut(s) 713, 743, 906, 923, 936, 953
Bsc4I CCNNNNNNNGG 5 cut(s) 63, 237, 451, 488, 933
Bse118I RCCGGY 2 cut(s) 380, 625
Bse1I ACTGG 1 cut(s) 1095
Bse21I CCTNAGG 1 cut(s) 152
BseAI TCCGGA 1 cut(s) 911
BseBI CCWGG 2 cut(s) 58, 190
BseDI CCNNGG 5 cut(s) 242, 516, 824, 837, 1029
BseGI GGATG 3 cut(s) 590, 656, 783
BseLI CCNNNNNNNGG 5 cut(s) 63, 237, 451, 488, 933
BseMII CTCAG 2 cut(s) 36, 140
BseNI ACTGG 1 cut(s) 1095
BseRI GAGGAG 5 cut(s) 259, 504, 509, 731, 734
BseSI GKGCMC 1 cut(s) 921
BseXI GCAGC 5 cut(s) 533, 625, 695, 910, 1077
BseYI CCCAGC 3 cut(s) 100, 445, 945
Bsh1236I CGCG 1 cut(s) 986
Bsh1285I CGRYCG 1 cut(s) 626
BshFI GGCC 6 cut(s) 67, 166, 179, 241, 810, 845
BshNI GGYRCC 1 cut(s) 454
BshTI ACCGGT 2 cut(s) 380, 625
BsiEI CGRYCG 1 cut(s) 626
BsiHKCI CYCGRG 1 cut(s) 667
BsiSI CCGG 5 cut(s) 293, 381, 626, 846, 912
BslFI GGGAC 1 cut(s) 413
BslI CCNNNNNNNGG 5 cut(s) 63, 237, 451, 488, 933
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 1 cut(s) 413
BsnI GGCC 6 cut(s) 67, 166, 179, 241, 810, 845
BsoBI CYCGRG 1 cut(s) 667
Bsp119I TTCGAA 1 cut(s) 468
Bsp1286I GDGCHC 1 cut(s) 921
Bsp13I TCCGGA 1 cut(s) 911
Bsp143I GATC 6 cut(s) 3, 209, 474, 544, 876, 987
Bsp19I CCATGG 2 cut(s) 824, 837
BspANI GGCC 6 cut(s) 67, 166, 179, 241, 810, 845
BspCNI CTCAG 2 cut(s) 37, 139
BspEI TCCGGA 1 cut(s) 911
BspFNI CGCG 1 cut(s) 986
BspLI GGNNCC 5 cut(s) 158, 429, 456, 634, 1055
BspMAI CTGCAG 1 cut(s) 900
BspPI GGATC 1 cut(s) 884
BspT104I TTCGAA 1 cut(s) 468
BspT107I GGYRCC 1 cut(s) 454
BsrFI RCCGGY 2 cut(s) 380, 625
BsrI ACTGG 1 cut(s) 1095
BssAI RCCGGY 2 cut(s) 380, 625
BssECI CCNNGG 5 cut(s) 242, 516, 824, 837, 1029
BssMI GATC 6 cut(s) 3, 209, 474, 544, 876, 987
BssNI GRCGYC 2 cut(s) 711, 834
BssSI CACGAG 1 cut(s) 324
BssT1I CCWWGG 3 cut(s) 824, 837, 1029
Bst2BI CACGAG 1 cut(s) 324
Bst2UI CCWGG 2 cut(s) 58, 190
Bst4CI ACNGT 2 cut(s) 317, 517
Bst6I CTCTTC 1 cut(s) 405
BstACI GRCGYC 2 cut(s) 711, 834
BstBI TTCGAA 1 cut(s) 468
BstC8I GCNNGC 5 cut(s) 106, 177, 744, 812, 984
BstDEI CTNAG 4 cut(s) 45, 126, 152, 696
BstDSI CCRYGG 3 cut(s) 516, 824, 837
BstENI CCTNNNNNAGG 1 cut(s) 235
BstF5I GGATG 3 cut(s) 590, 656, 783
BstFNI CGCG 1 cut(s) 986
BstH2I RGCGCY 2 cut(s) 77, 611
BstHHI GCGC 4 cut(s) 76, 610, 662, 688
BstKTI GATC 6 cut(s) 6, 212, 477, 547, 879, 990
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 6 cut(s) 3, 209, 474, 544, 876, 987
BstMCI CGRYCG 1 cut(s) 626
BstMWI GCNNNNNNNGC 8 cut(s) 73, 172, 337, 403, 651, 743, 788, 842
BstNI CCWGG 2 cut(s) 58, 190
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 2 cut(s) 56, 188
BstSFI CTRYAG 2 cut(s) 560, 896
BstSLI GKGCMC 1 cut(s) 921
BstUI CGCG 1 cut(s) 986
BstV1I GCAGC 5 cut(s) 533, 625, 695, 910, 1077
BstX2I RGATCY 1 cut(s) 544
BstYI RGATCY 1 cut(s) 544
Bsu36I CCTNAGG 1 cut(s) 152
BsuRI GGCC 6 cut(s) 67, 166, 179, 241, 810, 845
BtgI CCRYGG 3 cut(s) 516, 824, 837
BtrI CACGTC 1 cut(s) 1006
BtsCI GGATG 3 cut(s) 590, 656, 783
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 2 cut(s) 456, 1102
Cac8I GCNNGC 5 cut(s) 106, 177, 744, 812, 984
CfoI GCGC 4 cut(s) 76, 610, 662, 688
Cfr10I RCCGGY 2 cut(s) 380, 625
Cfr13I GGNCC 3 cut(s) 66, 427, 808
CseI GACGC 2 cut(s) 411, 842
Csp6I GTAC 1 cut(s) 61
CspAI ACCGGT 2 cut(s) 380, 625
CviAII CATG 6 cut(s) 121, 256, 281, 825, 838, 973
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 4 cut(s) 45, 126, 152, 696
DpnI GATC 6 cut(s) 5, 211, 476, 546, 878, 989
DpnII GATC 6 cut(s) 3, 209, 474, 544, 876, 987
DraIII CACNNNGTG 1 cut(s) 772
EaeI YGGCCR 2 cut(s) 164, 843
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Eco130I CCWWGG 3 cut(s) 824, 837, 1029
Eco47I GGWCC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 753
Eco81I CCTNAGG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 667
EcoNI CCTNNNNNAGG 1 cut(s) 235
EcoRI GAATTC 1 cut(s) 568
EcoRII CCWGG 2 cut(s) 56, 188
EcoT14I CCWWGG 3 cut(s) 824, 837, 1029
EcoT22I ATGCAT 1 cut(s) 656
ErhI CCWWGG 3 cut(s) 824, 837, 1029
FaeI CATG 6 cut(s) 124, 259, 284, 828, 841, 976
FaqI GGGAC 1 cut(s) 413
FatI CATG 6 cut(s) 120, 255, 280, 824, 837, 972
FauI CCCGC 4 cut(s) 195, 583, 875, 1070
FbaI TGATCA 2 cut(s) 3, 474
Fnu4HI GCNGC 9 cut(s) 167, 366, 458, 522, 611, 614, 684, 899, 1066
FokI GGATG 3 cut(s) 577, 663, 790
Fsp4HI GCNGC 9 cut(s) 167, 366, 458, 522, 611, 614, 684, 899, 1066
FspBI CTAG 1 cut(s) 260
GlaI GCGC 4 cut(s) 75, 609, 661, 687
GluI GCNGC 9 cut(s) 167, 366, 458, 522, 611, 614, 684, 899, 1066
GsaI CCCAGC 3 cut(s) 104, 449, 949
GsuI CTGGAG 2 cut(s) 1072, 1078
HaeII RGCGCY 2 cut(s) 77, 611
HaeIII GGCC 6 cut(s) 67, 166, 179, 241, 810, 845
HapII CCGG 5 cut(s) 293, 381, 626, 846, 912
HgaI GACGC 2 cut(s) 411, 842
HhaI GCGC 4 cut(s) 76, 610, 662, 688
Hin1I GRCGYC 2 cut(s) 711, 834
Hin1II CATG 6 cut(s) 124, 259, 284, 828, 841, 976
Hin6I GCGC 4 cut(s) 74, 608, 660, 686
HinP1I GCGC 4 cut(s) 74, 608, 660, 686
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 8 cut(s) 140, 193, 247, 391, 728, 758, 908, 1025
HpaII CCGG 5 cut(s) 293, 381, 626, 846, 912
HphI GGTGA 2 cut(s) 307, 961
Hpy188I TCNGA 8 cut(s) 129, 225, 306, 336, 351, 733, 757, 907
Hpy188III TCNNGA 1 cut(s) 912
Hpy99I CGWCG 5 cut(s) 427, 716, 836, 857, 1007
HpyCH4III ACNGT 2 cut(s) 317, 517
HpyCH4IV ACGT 3 cut(s) 711, 995, 1005
HpyCH4V TGCA 4 cut(s) 175, 286, 654, 898
HpyF10VI GCNNNNNNNGC 8 cut(s) 73, 172, 337, 403, 651, 743, 788, 842
HpyF3I CTNAG 4 cut(s) 45, 126, 152, 696
HpySE526I ACGT 3 cut(s) 711, 995, 1005
Hsp92I GRCGYC 2 cut(s) 711, 834
Hsp92II CATG 6 cut(s) 124, 259, 284, 828, 841, 976
HspAI GCGC 4 cut(s) 74, 608, 660, 686
Kpn2I TCCGGA 1 cut(s) 911
Ksp22I TGATCA 2 cut(s) 3, 474
Kzo9I GATC 6 cut(s) 3, 209, 474, 544, 876, 987
LmnI GCTCC 2 cut(s) 496, 1097
Lsp1109I GCAGC 5 cut(s) 533, 625, 695, 910, 1077
LweI GCATC 3 cut(s) 352, 641, 663
MaeI CTAG 1 cut(s) 260
MaeII ACGT 3 cut(s) 711, 995, 1005
MaeIII GTNAC 2 cut(s) 85, 436
MalI GATC 6 cut(s) 5, 211, 476, 546, 878, 989
MboI GATC 6 cut(s) 3, 209, 474, 544, 876, 987
MboII GAAGA 1 cut(s) 392
MflI RGATCY 1 cut(s) 544
MhlI GDGCHC 1 cut(s) 921
MluCI AATT 3 cut(s) 470, 568, 618
MlyI GAGTC 6 cut(s) 187, 256, 385, 737, 752, 1034
MmeI TCCRAC 3 cut(s) 248, 374, 628
Mph1103I ATGCAT 1 cut(s) 656
MroI TCCGGA 1 cut(s) 911
MseI TTAA 2 cut(s) 234, 674
MspA1I CMGCKG 3 cut(s) 44, 524, 882
MspI CCGG 5 cut(s) 293, 381, 626, 846, 912
MspR9I CCNGG 2 cut(s) 58, 190
MvaI CCWGG 2 cut(s) 58, 190
MvnI CGCG 1 cut(s) 986
MwoI GCNNNNNNNGC 8 cut(s) 73, 172, 337, 403, 651, 743, 788, 842
NcoI CCATGG 2 cut(s) 824, 837
NdeII GATC 6 cut(s) 3, 209, 474, 544, 876, 987
NlaIII CATG 6 cut(s) 124, 259, 284, 828, 841, 976
NlaIV GGNNCC 5 cut(s) 158, 429, 456, 634, 1055
NmeAIII GCCGAG 1 cut(s) 267
NmuCI GTSAC 2 cut(s) 85, 436
NsiI ATGCAT 1 cut(s) 656
NspI RCATGY 1 cut(s) 259
NspV TTCGAA 1 cut(s) 468
PfeI GAWTC 2 cut(s) 140, 908
PinAI ACCGGT 2 cut(s) 380, 625
PkrI GCNGC 9 cut(s) 168, 367, 459, 523, 612, 615, 685, 900, 1067
PleI GAGTC 6 cut(s) 187, 255, 385, 736, 752, 1033
PpsI GAGTC 6 cut(s) 187, 255, 385, 736, 752, 1033
Psp6I CCWGG 2 cut(s) 56, 188
PspFI CCCAGC 3 cut(s) 100, 445, 945
PspGI CCWGG 2 cut(s) 56, 188
PspN4I GGNNCC 5 cut(s) 158, 429, 456, 634, 1055
PspPI GGNCC 3 cut(s) 66, 427, 808
PsrI GAACNNNNNNTAC 2 cut(s) 776, 808
PstI CTGCAG 1 cut(s) 900
PsuI RGATCY 1 cut(s) 544
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SaqAI TTAA 2 cut(s) 234, 674
SatI GCNGC 9 cut(s) 167, 366, 458, 522, 611, 614, 684, 899, 1066
Sau3AI GATC 6 cut(s) 3, 209, 474, 544, 876, 987
Sau96I GGNCC 3 cut(s) 66, 427, 808
SchI GAGTC 6 cut(s) 187, 256, 385, 737, 752, 1034
ScrFI CCNGG 2 cut(s) 58, 190
SduI GDGCHC 1 cut(s) 921
SfaNI GCATC 3 cut(s) 352, 641, 663
SfcI CTRYAG 2 cut(s) 560, 896
SfuI TTCGAA 1 cut(s) 468
SinI GGWCC 1 cut(s) 427
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 3 cut(s) 470, 568, 618
SspMI CTAG 1 cut(s) 260
StyD4I CCNGG 2 cut(s) 56, 188
StyI CCWWGG 3 cut(s) 824, 837, 1029
TaaI ACNGT 2 cut(s) 317, 517
TaiI ACGT 3 cut(s) 714, 998, 1008
TaqI TCGA 7 cut(s) 196, 208, 360, 468, 622, 714, 831
TaqII GACCGA 1 cut(s) 415
TasI AATT 3 cut(s) 470, 568, 618
TauI GCSGC 4 cut(s) 169, 368, 460, 613
TfiI GAWTC 2 cut(s) 140, 908
Tru1I TTAA 2 cut(s) 234, 674
Tru9I TTAA 2 cut(s) 234, 674
TscAI CASTG 2 cut(s) 456, 1102
TseFI GTSAC 2 cut(s) 85, 436
TseI GCWGC 5 cut(s) 521, 613, 683, 898, 1065
Tsp45I GTSAC 2 cut(s) 85, 436
TspDTI ATGAA 2 cut(s) 989, 1025
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 2 cut(s) 456, 1102
VpaK11BI GGWCC 1 cut(s) 427
XagI CCTNNNNNAGG 1 cut(s) 235
XapI RAATTY 1 cut(s) 568
XceI RCATGY 1 cut(s) 259
XcmI CCANNNNNNNNNTGG 1 cut(s) 822
XspI CTAG 1 cut(s) 260
ZraI GACGTC 1 cut(s) 712
Zsp2I ATGCAT 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.