AT1G29195

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016607
Physical Location & Seq
Reverse (-)
10202414 .. 10203491
1078 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G29195.1

Sequence Viewer

Length: 582 bp
ATGAAGGAGACAATCAGGTGTTGCATCGCTTGCATTCTACCGTGTGGAGCGCTTGACGTGATCAGAATCGTTCACTCAAACGGACACGTGGAGGAGATCAGTGGCACGATCACGGCCAGCGAAATCATGAAGGCTCACCCGAAGCACGTACTCAAGAAACCATCTTCTCCAACGTCAGATCACGACGAGCGTGACGTCATCTCAGCCACAAAGATCGTCATCGTCCCTCCCGAAGCTGAGCTCCAACGTGGTAAGATTTATTTCCTCATGCCTGCTACTAAATCTGATAAATGCGCCGGAGGAGGAAAGATCCGCCGGGAAAAAAGTAACGCAAACGCCGTCGTCAAGAAACGGTCACAGTATCGCCGGCAGCATCGTGACGGTGATGAGAAATGTAACGGCGGCGAAAGTAATGACGTTAAAGATAAAAACTACGAGAAGGATTTATTGATCTCGTCTGATCGATACTTGACGGAGATATTATCGGAGAAAGTGGCGACGCAAAAAGATCGGAGGAAAGGACGCGTCGGCGTTTGGAGGCCGCACTTAGAAAGCATATCGGAGATCATAACTGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

193

Amino Acids

21.65

Weight (kDa)

8.98

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 7 - 188 1.2e-38 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016607)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29195
fragaria_vesca FvH4_3g07740
malus_domestica MD05G1300400.v1.1 MD10G1279500.v1.1
prunus_persica Prupe.4G061900_v2.0.a1 Prupe.4G061900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0010861
rosa_laevigata RLG00000031792
rosa_multiflora Rmu_sc0000109.1_g000007
rosa_rugosa Rorug04G0453300
rosa_samantha Rh5CG092700 Rh5DG080400
rosa_wichuraiana Rw0G017930 Rw0G022220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 198
AccII CGCG 1 cut(s) 525
AciI CCGC 3 cut(s) 313, 402, 542
AclWI GGATC 1 cut(s) 304
AcoI YGGCCR 1 cut(s) 114
AcvI CACGTG 1 cut(s) 88
AcyI GRCGYC 1 cut(s) 195
AfaI GTAC 1 cut(s) 150
AfeI AGCGCT 1 cut(s) 51
AflIII ACRYGT 2 cut(s) 85, 523
AjiI CACGTC 1 cut(s) 58
AluBI AGCT 2 cut(s) 236, 241
AluI AGCT 2 cut(s) 236, 241
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 1 cut(s) 304
Aor51HI AGCGCT 1 cut(s) 51
AoxI GGCC 2 cut(s) 114, 539
ApeKI GCWGC 1 cut(s) 370
AspLEI GCGC 2 cut(s) 52, 296
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 2 cut(s) 128, 395
BanII GRGCYC 1 cut(s) 243
BbrPI CACGTG 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 382
BccI CCATC 1 cut(s) 169
BceAI ACGGC 3 cut(s) 129, 323, 415
BcgI CGANNNNNNTGC 2 cut(s) 491, 525
BclI TGATCA 1 cut(s) 60
BcnI CCSGG 1 cut(s) 317
BcoDI GTCTC 1 cut(s) 2
BfoI RGCGCY 1 cut(s) 53
BisI GCNGC 3 cut(s) 371, 403, 542
BlpI GCTNAGC 1 cut(s) 237
BlsI GCNGC 3 cut(s) 372, 404, 543
Bme1390I CCNGG 1 cut(s) 317
BmgBI CACGTC 1 cut(s) 58
BmrFI CCNGG 1 cut(s) 317
BmsI GCATC 2 cut(s) 33, 382
Bpu1102I GCTNAGC 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 317
Bsa29I ATCGAT 1 cut(s) 463
BsaAI YACGTR 2 cut(s) 88, 148
BsaBI GATNNNNATC 2 cut(s) 65, 218
BsaHI GRCGYC 1 cut(s) 195
Bse118I RCCGGY 1 cut(s) 366
Bse8I GATNNNNATC 2 cut(s) 65, 218
BseCI ATCGAT 1 cut(s) 463
BseJI GATNNNNATC 2 cut(s) 65, 218
BseMII CTCAG 2 cut(s) 216, 228
BseRI GAGGAG 2 cut(s) 107, 315
BseXI GCAGC 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 525
BshFI GGCC 2 cut(s) 116, 541
BshVI ATCGAT 1 cut(s) 463
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 3 cut(s) 297, 316, 367
BslFI GGGAC 1 cut(s) 209
BsmAI GTCTC 1 cut(s) 2
BsmFI GGGAC 1 cut(s) 209
BsmI GAATGC 1 cut(s) 33
BsnI GGCC 2 cut(s) 116, 541
Bsp1286I GDGCHC 1 cut(s) 243
Bsp1720I GCTNAGC 1 cut(s) 237
BspACI CCGC 3 cut(s) 313, 402, 542
BspANI GGCC 2 cut(s) 116, 541
BspCNI CTCAG 2 cut(s) 215, 229
BspDI ATCGAT 1 cut(s) 463
BspFNI CGCG 1 cut(s) 525
BspHI TCATGA 1 cut(s) 126
BspPI GGATC 1 cut(s) 304
BsrFI RCCGGY 1 cut(s) 366
BssAI RCCGGY 1 cut(s) 366
BssNI GRCGYC 1 cut(s) 195
Bst4CI ACNGT 4 cut(s) 42, 354, 360, 383
BstACI GRCGYC 1 cut(s) 195
BstAPI GCANNNNNTGC 1 cut(s) 30
BstBAI YACGTR 2 cut(s) 88, 148
BstC8I GCNNGC 4 cut(s) 31, 118, 273, 368
BstDEI CTNAG 3 cut(s) 202, 237, 547
BstFNI CGCG 1 cut(s) 525
BstH2I RGCGCY 1 cut(s) 53
BstHHI GCGC 2 cut(s) 52, 296
BstMAI GTCTC 1 cut(s) 2
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 315
BstUI CGCG 1 cut(s) 525
BstV1I GCAGC 1 cut(s) 382
BstX2I RGATCY 1 cut(s) 309
BstYI RGATCY 1 cut(s) 309
Bsu15I ATCGAT 1 cut(s) 463
BsuRI GGCC 2 cut(s) 116, 541
BsuTUI ATCGAT 1 cut(s) 463
BtgZI GCGATG 1 cut(s) 10
BtrI CACGTC 1 cut(s) 58
BtsIMutI CAGTG 1 cut(s) 106
Cac8I GCNNGC 4 cut(s) 31, 118, 273, 368
CciI TCATGA 1 cut(s) 126
CfoI GCGC 2 cut(s) 52, 296
Cfr10I RCCGGY 1 cut(s) 366
ClaI ATCGAT 1 cut(s) 463
CseI GACGC 3 cut(s) 508, 514, 531
Csp6I GTAC 1 cut(s) 149
CviAII CATG 2 cut(s) 127, 268
CviJI RGCY 6 cut(s) 116, 134, 206, 236, 241, 541
CviKI_1 RGCY 6 cut(s) 116, 134, 206, 236, 241, 541
CviQI GTAC 1 cut(s) 149
DdeI CTNAG 3 cut(s) 202, 237, 547
EaeI YGGCCR 1 cut(s) 114
EciI GGCGGA 1 cut(s) 302
Ecl136II GAGCTC 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 243
Eco47III AGCGCT 1 cut(s) 51
Eco53kI GAGCTC 1 cut(s) 241
Eco72I CACGTG 1 cut(s) 88
EcoICRI GAGCTC 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 243
FaeI CATG 2 cut(s) 130, 271
FaiI YATR 4 cut(s) 128, 269, 557, 569
FaqI GGGAC 1 cut(s) 209
FatI CATG 2 cut(s) 126, 267
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 3 cut(s) 371, 403, 542
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 3 cut(s) 371, 403, 542
GlaI GCGC 2 cut(s) 51, 295
GluI GCNGC 3 cut(s) 371, 403, 542
HaeII RGCGCY 1 cut(s) 53
HaeIII GGCC 2 cut(s) 116, 541
HapII CCGG 3 cut(s) 297, 316, 367
HgaI GACGC 3 cut(s) 508, 514, 531
HhaI GCGC 2 cut(s) 52, 296
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 2 cut(s) 130, 271
Hin6I GCGC 2 cut(s) 50, 294
HinP1I GCGC 2 cut(s) 50, 294
HinfI GANTC 1 cut(s) 66
HpaII CCGG 3 cut(s) 297, 316, 367
HphI GGTGA 2 cut(s) 128, 395
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 7 cut(s) 65, 178, 286, 460, 487, 513, 562
Hpy188III TCNNGA 6 cut(s) 127, 154, 182, 230, 346, 377
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 4 cut(s) 188, 344, 502, 530
HpyAV CCTTC 2 cut(s) 124, 433
HpyCH4III ACNGT 4 cut(s) 42, 354, 360, 383
HpyCH4IV ACGT 7 cut(s) 57, 87, 147, 173, 195, 247, 417
HpyCH4V TGCA 2 cut(s) 24, 33
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpyF3I CTNAG 3 cut(s) 202, 237, 547
HpySE526I ACGT 7 cut(s) 57, 87, 147, 173, 195, 247, 417
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 2 cut(s) 130, 271
HspAI GCGC 2 cut(s) 50, 294
KroI GCCGGC 1 cut(s) 366
KroNI GCCGGC 1 cut(s) 368
Ksp22I TGATCA 1 cut(s) 60
LmnI GCTCC 2 cut(s) 47, 246
LpnPI CCDG 5 cut(s) 130, 285, 310, 329, 380
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 2 cut(s) 33, 382
MaeII ACGT 7 cut(s) 57, 87, 147, 173, 195, 247, 417
MaeIII GTNAC 5 cut(s) 191, 326, 354, 377, 395
MboII GAAGA 1 cut(s) 156
MflI RGATCY 1 cut(s) 309
MhlI GDGCHC 1 cut(s) 243
MluI ACGCGT 1 cut(s) 523
MmeI TCCRAC 2 cut(s) 194, 268
MnlI CCTC 7 cut(s) 85, 237, 275, 293, 296, 507, 531
MroNI GCCGGC 1 cut(s) 366
MseI TTAA 1 cut(s) 420
MspI CCGG 3 cut(s) 297, 316, 367
MspR9I CCNGG 1 cut(s) 317
Mva1269I GAATGC 1 cut(s) 33
MvnI CGCG 1 cut(s) 525
MwoI GCNNNNNNNGC 1 cut(s) 30
NaeI GCCGGC 1 cut(s) 368
NciI CCSGG 1 cut(s) 317
NgoMIV GCCGGC 1 cut(s) 366
NlaIII CATG 2 cut(s) 130, 271
NmuCI GTSAC 3 cut(s) 191, 354, 377
PagI TCATGA 1 cut(s) 126
PcsI WCGNNNNNNNCGW 3 cut(s) 192, 228, 336
PctI GAATGC 1 cut(s) 33
PdiI GCCGGC 1 cut(s) 368
PfeI GAWTC 1 cut(s) 66
PkrI GCNGC 3 cut(s) 372, 404, 543
PmaCI CACGTG 1 cut(s) 88
PmlI CACGTG 1 cut(s) 88
Ppu21I YACGTR 2 cut(s) 88, 148
Psp124BI GAGCTC 1 cut(s) 243
PspCI CACGTG 1 cut(s) 88
PsuI RGATCY 1 cut(s) 309
RsaI GTAC 1 cut(s) 150
RsaNI GTAC 1 cut(s) 149
SacI GAGCTC 1 cut(s) 243
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 3 cut(s) 371, 403, 542
ScrFI CCNGG 1 cut(s) 317
SduI GDGCHC 1 cut(s) 243
SfaNI GCATC 2 cut(s) 33, 382
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
SsiI CCGC 3 cut(s) 313, 402, 542
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 315
TaaI ACNGT 4 cut(s) 42, 354, 360, 383
TaiI ACGT 7 cut(s) 60, 90, 150, 176, 198, 250, 420
TaqI TCGA 1 cut(s) 463
TauI GCSGC 2 cut(s) 405, 544
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 3 cut(s) 191, 354, 377
TseI GCWGC 1 cut(s) 370
Tsp45I GTSAC 3 cut(s) 191, 354, 377
TspDTI ATGAA 2 cut(s) 17, 143
TspGWI ACGGA 2 cut(s) 96, 488
TspRI CASTG 1 cut(s) 106
ZraI GACGTC 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.