MD05G1300400.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016607
Physical Location & Seq
Forward (+)
43357440 .. 43358225
786 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1300400.v1.1.491

Sequence Viewer

Length: 597 bp
ATGAAAAACACCATCAGGTGCTGCATCTCTTGCATTCTACCATGTGGAGCTTTGGACGTGATTCGAGTCGTACACTCTAACGGCAGAGTCGAGGAAATCAGCGGCACAATCCGAGCCAGCGAGATCATGAAGGCGTACCCTAAGCACGTCCTCAAGAAGCCCTCCTCGTCCGCGTCCGATCACGACGGGGTCGTCCCCAAGATCGTGATCGTTCCTCCAGACGCGGAACTCCAACGCGGCAAGATTTACTTCCTCATGCCTATGCCTGCTTCTTCGAACACGTCTGAAAAGGCGGGGAGGACAAGATCGTCGTCGGCAAAGAAGAAGCGGATTAAAGACGCCGAAAACAACAACATCACAAACAACAGCGTCGCCATCACCAACCTGTTGATAAATGATCGGTACTTGAGCGAAATACTGTCGGAAAAGCATTCGTCGCAGCGGGATCGGCGGCGAGGACGTGTTGGGGTTTGGAGGCCGCACTTAGAGAGCATTTGTGAGTCACCAAGTGATGTGGAAAGAAGAAATAATATACAAACAACTTCTATGAGAGGGTCGATCTCTGTTATTGTGGTGGGTTCTCACCCGACTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

199

Amino Acids

21.95

Weight (kDa)

9.93

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 7 - 167 2.5e-38 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016607)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29195
fragaria_vesca FvH4_3g07740
malus_domestica MD05G1300400.v1.1 MD10G1279500.v1.1
prunus_persica Prupe.4G061900_v2.0.a1 Prupe.4G061900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0010861
rosa_laevigata RLG00000031792
rosa_multiflora Rmu_sc0000109.1_g000007
rosa_rugosa Rorug04G0453300
rosa_samantha Rh5CG092700 Rh5DG080400
rosa_wichuraiana Rw0G017930 Rw0G022220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 191, 307
AccII CGCG 3 cut(s) 173, 224, 237
AciI CCGC 9 cut(s) 102, 171, 224, 237, 293, 328, 442, 451, 479
AclWI GGATC 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 339
AdeI CACNNNGTG 1 cut(s) 509
AfaI GTAC 3 cut(s) 72, 137, 404
AflIII ACRYGT 2 cut(s) 279, 460
AjiI CACGTC 4 cut(s) 58, 148, 282, 461
AluBI AGCT 1 cut(s) 50
AluI AGCT 1 cut(s) 50
AlwI GGATC 1 cut(s) 453
AlwNI CAGNNNCTG 1 cut(s) 21
AoxI GGCC 1 cut(s) 476
ApeKI GCWGC 2 cut(s) 21, 439
AsuHPI GGTGA 3 cut(s) 370, 495, 575
AsuII TTCGAA 1 cut(s) 275
BbvI GCAGC 2 cut(s) 8, 451
BccI CCATC 2 cut(s) 20, 383
BceAI ACGGC 1 cut(s) 97
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BfaI CTAG 1 cut(s) 591
BisI GCNGC 6 cut(s) 22, 103, 238, 440, 452, 479
BlsI GCNGC 6 cut(s) 23, 104, 239, 441, 453, 480
BmgBI CACGTC 4 cut(s) 58, 148, 282, 461
BmsI GCATC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 201
Bpu10I CCTNAGC 1 cut(s) 141
Bpu14I TTCGAA 1 cut(s) 275
BpuEI CTTGAG 2 cut(s) 137, 427
BsaBI GATNNNNATC 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 339
Bse8I GATNNNNATC 1 cut(s) 206
BseJI GATNNNNATC 1 cut(s) 206
BseRI GAGGAG 1 cut(s) 154
BseXI GCAGC 2 cut(s) 8, 451
Bsh1236I CGCG 3 cut(s) 173, 224, 237
BshFI GGCC 1 cut(s) 478
BslFI GGGAC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 179
BsmI GAATGC 2 cut(s) 33, 430
BsnI GGCC 1 cut(s) 478
Bsp119I TTCGAA 1 cut(s) 275
Bsp143I GATC 8 cut(s) 123, 178, 201, 207, 305, 397, 445, 558
BspACI CCGC 9 cut(s) 102, 171, 224, 237, 293, 328, 442, 451, 479
BspANI GGCC 1 cut(s) 478
BspFNI CGCG 3 cut(s) 173, 224, 237
BspHI TCATGA 1 cut(s) 126
BspPI GGATC 1 cut(s) 453
BspT104I TTCGAA 1 cut(s) 275
BssMI GATC 8 cut(s) 123, 178, 201, 207, 305, 397, 445, 558
BssNI GRCGYC 1 cut(s) 339
Bst4CI ACNGT 1 cut(s) 420
BstACI GRCGYC 1 cut(s) 339
BstAPI GCANNNNNTGC 1 cut(s) 30
BstBI TTCGAA 1 cut(s) 275
BstC8I GCNNGC 2 cut(s) 118, 267
BstDEI CTNAG 2 cut(s) 141, 484
BstFNI CGCG 3 cut(s) 173, 224, 237
BstKTI GATC 8 cut(s) 126, 181, 204, 210, 308, 400, 448, 561
BstMBI GATC 8 cut(s) 123, 178, 201, 207, 305, 397, 445, 558
BstMWI GCNNNNNNNGC 3 cut(s) 30, 436, 448
BstUI CGCG 3 cut(s) 173, 224, 237
BstV1I GCAGC 2 cut(s) 8, 451
BsuRI GGCC 1 cut(s) 478
BtrI CACGTC 4 cut(s) 58, 148, 282, 461
Cac8I GCNNGC 2 cut(s) 118, 267
CaiI CAGNNNCTG 1 cut(s) 21
CciI TCATGA 1 cut(s) 126
CseI GACGC 4 cut(s) 162, 230, 347, 358
Csp6I GTAC 3 cut(s) 71, 136, 403
CspCI CAANNNNNGTGG 2 cut(s) 495, 530
CviAII CATG 3 cut(s) 42, 127, 256
CviJI RGCY 4 cut(s) 50, 116, 160, 478
CviKI_1 RGCY 4 cut(s) 50, 116, 160, 478
CviQI GTAC 3 cut(s) 71, 136, 403
DdeI CTNAG 2 cut(s) 141, 484
DpnI GATC 8 cut(s) 125, 180, 203, 209, 307, 399, 447, 560
DpnII GATC 8 cut(s) 123, 178, 201, 207, 305, 397, 445, 558
DraIII CACNNNGTG 1 cut(s) 509
DrdI GACNNNNNNGTC 2 cut(s) 191, 307
DseDI GACNNNNNNGTC 2 cut(s) 191, 307
FaeI CATG 3 cut(s) 45, 130, 259
FaiI YATR 6 cut(s) 43, 128, 257, 263, 533, 548
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 1 cut(s) 179
FatI CATG 3 cut(s) 41, 126, 255
FauI CCCGC 2 cut(s) 286, 435
Fnu4HI GCNGC 6 cut(s) 22, 103, 238, 440, 452, 479
Fsp4HI GCNGC 6 cut(s) 22, 103, 238, 440, 452, 479
FspBI CTAG 1 cut(s) 591
GluI GCNGC 6 cut(s) 22, 103, 238, 440, 452, 479
GsuI CTGGAG 1 cut(s) 201
HaeIII GGCC 1 cut(s) 478
HgaI GACGC 4 cut(s) 162, 230, 347, 358
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 3 cut(s) 45, 130, 259
HinfI GANTC 4 cut(s) 61, 66, 87, 500
HphI GGTGA 3 cut(s) 370, 495, 575
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 4 cut(s) 113, 178, 286, 424
Hpy188III TCNNGA 5 cut(s) 127, 154, 182, 205, 218
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 5 cut(s) 188, 313, 316, 374, 439
HpyAV CCTTC 1 cut(s) 124
HpyCH4III ACNGT 1 cut(s) 420
HpyCH4IV ACGT 4 cut(s) 57, 147, 281, 460
HpyCH4V TGCA 2 cut(s) 24, 33
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 436, 448
HpyF3I CTNAG 2 cut(s) 141, 484
HpySE526I ACGT 4 cut(s) 57, 147, 281, 460
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 3 cut(s) 45, 130, 259
Kzo9I GATC 8 cut(s) 123, 178, 201, 207, 305, 397, 445, 558
LmnI GCTCC 1 cut(s) 47
LpnPI CCDG 4 cut(s) 130, 231, 279, 398
Lsp1109I GCAGC 2 cut(s) 8, 451
LweI GCATC 1 cut(s) 33
MaeI CTAG 1 cut(s) 591
MaeII ACGT 4 cut(s) 57, 147, 281, 460
MaeIII GTNAC 1 cut(s) 501
MalI GATC 8 cut(s) 125, 180, 203, 209, 307, 399, 447, 560
MboI GATC 8 cut(s) 123, 178, 201, 207, 305, 397, 445, 558
MboII GAAGA 3 cut(s) 264, 334, 534
MlyI GAGTC 3 cut(s) 75, 96, 509
MmeI TCCRAC 2 cut(s) 256, 402
MseI TTAA 1 cut(s) 333
MslI CAYNNNNRTG 1 cut(s) 260
MspA1I CMGCKG 2 cut(s) 102, 442
Mva1269I GAATGC 2 cut(s) 33, 430
MvnI CGCG 3 cut(s) 173, 224, 237
MwoI GCNNNNNNNGC 3 cut(s) 30, 436, 448
NdeII GATC 8 cut(s) 123, 178, 201, 207, 305, 397, 445, 558
NlaIII CATG 3 cut(s) 45, 130, 259
NmuCI GTSAC 1 cut(s) 501
NspV TTCGAA 1 cut(s) 275
PagI TCATGA 1 cut(s) 126
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 189
PctI GAATGC 2 cut(s) 33, 430
PfeI GAWTC 1 cut(s) 61
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 6 cut(s) 23, 104, 239, 441, 453, 480
PleI GAGTC 3 cut(s) 74, 95, 508
PpsI GAGTC 3 cut(s) 74, 95, 508
PstNI CAGNNNCTG 1 cut(s) 21
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 3 cut(s) 72, 137, 404
RsaNI GTAC 3 cut(s) 71, 136, 403
RseI CAYNNNNRTG 1 cut(s) 260
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 6 cut(s) 22, 103, 238, 440, 452, 479
Sau3AI GATC 8 cut(s) 123, 178, 201, 207, 305, 397, 445, 558
SchI GAGTC 3 cut(s) 75, 96, 509
SetI ASST 7 cut(s) 20, 52, 60, 150, 284, 387, 463
SfaNI GCATC 1 cut(s) 33
SfuI TTCGAA 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 2 cut(s) 152, 406
SmoI CTYRAG 2 cut(s) 152, 406
SsiI CCGC 9 cut(s) 102, 171, 224, 237, 293, 328, 442, 451, 479
SspMI CTAG 1 cut(s) 591
TaaI ACNGT 1 cut(s) 420
TaiI ACGT 4 cut(s) 60, 150, 284, 463
TaqI TCGA 4 cut(s) 64, 90, 275, 557
TauI GCSGC 4 cut(s) 105, 240, 454, 481
TfiI GAWTC 1 cut(s) 61
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TseFI GTSAC 1 cut(s) 501
TseI GCWGC 2 cut(s) 21, 439
Tsp45I GTSAC 1 cut(s) 501
TspDTI ATGAA 2 cut(s) 17, 143
Tth111I GACNNNGTC 1 cut(s) 188
XspI CTAG 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.