FvH4_3g07740

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016607
Physical Location & Seq
Forward (+)
4547396 .. 4547905
510 bp
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UTR
Exon/CDS
Intron
FvH4_3g07740.t1

Sequence Viewer

Length: 510 bp
ATGAAGAACGCAATCCGATGCTGCATCTCTTGCATTCTACCATGCGGAGCTCTCGACGTGATCCGAGTCGTTCACTGCAACGGCCGAGTGGAAGAGATCAGCGGCTCCATCCGCGCCAGCGATATCATGAAAGCTCACCCCAAGCACGTCCTCAAGAAGCCCTCTTCCTCGCCGTCAGATCACGGCGGTGTCGTCCCCAAGATCGTGATCGTTCCTCCCGACGCCGAGCTCCAGCGCGGCAAGATTTACTTCCTCATGCCAGTTCCTTCCGAGAAGACGACGACGAAGTCGACCAGAGTGAAGAAGAGGAAAGTCGAGGCGGGAAATAAGGACAGCTCCAACAGCGTCGTTATGAACAGTATTGCCATGACCAACCTTCTGATATCGGATCAGTACTTGAGTGAGATACTGTCGGAAAAGGTTTCTACGCAGAGGGATCGGCGGCGAGGACGTGTCGGTGTGTGGAGGCCTCACTTAGAGAGTATATGCGAGTCACCAAGTGATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

170

Amino Acids

18.63

Weight (kDa)

9.56

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 7 - 164 7.4e-37 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016607)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29195
fragaria_vesca FvH4_3g07740
malus_domestica MD05G1300400.v1.1 MD10G1279500.v1.1
prunus_persica Prupe.4G061900_v2.0.a1 Prupe.4G061900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0010861
rosa_laevigata RLG00000031792
rosa_multiflora Rmu_sc0000109.1_g000007
rosa_rugosa Rorug04G0453300
rosa_samantha Rh5CG092700 Rh5DG080400
rosa_wichuraiana Rw0G017930 Rw0G022220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 286
AccI GTMKAC 1 cut(s) 290
AccII CGCG 2 cut(s) 114, 237
AciI CCGC 7 cut(s) 45, 102, 112, 186, 237, 320, 442
AclWI GGATC 3 cut(s) 55, 396, 444
AcoI YGGCCR 1 cut(s) 82
AcyI GRCGYC 1 cut(s) 222
AdeI CACNNNGTG 1 cut(s) 500
AfaI GTAC 1 cut(s) 395
AflIII ACRYGT 1 cut(s) 451
AjiI CACGTC 3 cut(s) 58, 148, 452
AleI CACNNNNGTG 1 cut(s) 186
AluBI AGCT 4 cut(s) 50, 134, 229, 336
AluI AGCT 4 cut(s) 50, 134, 229, 336
Alw21I GWGCWC 2 cut(s) 52, 231
AlwI GGATC 3 cut(s) 55, 396, 444
AoxI GGCC 2 cut(s) 82, 467
ApeKI GCWGC 1 cut(s) 21
AspLEI GCGC 2 cut(s) 116, 237
AsuHPI GGTGA 2 cut(s) 128, 486
BanII GRGCYC 2 cut(s) 52, 231
BbsI GAAGAC 1 cut(s) 281
Bbv12I GWGCWC 2 cut(s) 52, 231
BbvI GCAGC 1 cut(s) 8
BccI CCATC 1 cut(s) 116
BceAI ACGGC 3 cut(s) 97, 157, 199
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BisI GCNGC 4 cut(s) 22, 103, 238, 443
BlsI GCNGC 4 cut(s) 23, 104, 239, 444
BmcAI AGTACT 1 cut(s) 395
BmgBI CACGTC 3 cut(s) 58, 148, 452
BmiI GGNNCC 1 cut(s) 106
BmsI GCATC 2 cut(s) 8, 33
BpiI GAAGAC 1 cut(s) 281
BpmI CTGGAG 1 cut(s) 215
BpuEI CTTGAG 2 cut(s) 137, 418
BsaBI GATNNNNATC 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 222
Bse1I ACTGG 1 cut(s) 260
Bse8I GATNNNNATC 1 cut(s) 206
BseGI GGATG 1 cut(s) 108
BseJI GATNNNNATC 1 cut(s) 206
BseNI ACTGG 1 cut(s) 260
BseX3I CGGCCG 1 cut(s) 82
BseXI GCAGC 1 cut(s) 8
Bsh1236I CGCG 2 cut(s) 114, 237
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 2 cut(s) 84, 469
BsiEI CGRYCG 1 cut(s) 85
BsiHKAI GWGCWC 2 cut(s) 52, 231
BslFI GGGAC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 179
BsmI GAATGC 1 cut(s) 33
BsnI GGCC 2 cut(s) 84, 469
Bsp1286I GDGCHC 2 cut(s) 52, 231
Bsp143I GATC 7 cut(s) 60, 96, 178, 201, 207, 388, 436
BspACI CCGC 7 cut(s) 45, 102, 112, 186, 237, 320, 442
BspANI GGCC 2 cut(s) 84, 469
BspFNI CGCG 2 cut(s) 114, 237
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 1 cut(s) 106
BspPI GGATC 3 cut(s) 55, 396, 444
BsrI ACTGG 1 cut(s) 260
BssMI GATC 7 cut(s) 60, 96, 178, 201, 207, 388, 436
BssNI GRCGYC 1 cut(s) 222
Bst4CI ACNGT 2 cut(s) 359, 411
Bst6I CTCTTC 3 cut(s) 87, 169, 299
BstACI GRCGYC 1 cut(s) 222
BstAPI GCANNNNNTGC 1 cut(s) 30
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 1 cut(s) 475
BstF5I GGATG 1 cut(s) 108
BstFNI CGCG 2 cut(s) 114, 237
BstHHI GCGC 2 cut(s) 116, 237
BstKTI GATC 7 cut(s) 63, 99, 181, 204, 210, 391, 439
BstMBI GATC 7 cut(s) 60, 96, 178, 201, 207, 388, 436
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 3 cut(s) 30, 111, 342
BstUI CGCG 2 cut(s) 114, 237
BstV1I GCAGC 1 cut(s) 8
BstV2I GAAGAC 1 cut(s) 281
BstZI CGGCCG 1 cut(s) 82
BsuRI GGCC 2 cut(s) 84, 469
BtrI CACGTC 3 cut(s) 58, 148, 452
BtsCI GGATG 1 cut(s) 108
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 118
CciI TCATGA 1 cut(s) 126
CfoI GCGC 2 cut(s) 116, 237
CseI GACGC 2 cut(s) 230, 334
Csp6I GTAC 1 cut(s) 394
CviAII CATG 4 cut(s) 42, 127, 256, 367
CviJI RGCY 8 cut(s) 50, 84, 105, 134, 160, 229, 336, 469
CviKI_1 RGCY 8 cut(s) 50, 84, 105, 134, 160, 229, 336, 469
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 1 cut(s) 475
DpnI GATC 7 cut(s) 62, 98, 180, 203, 209, 390, 438
DpnII GATC 7 cut(s) 60, 96, 178, 201, 207, 388, 436
DraIII CACNNNGTG 1 cut(s) 500
DrdI GACNNNNNNGTC 1 cut(s) 286
DseDI GACNNNNNNGTC 1 cut(s) 286
EaeI YGGCCR 1 cut(s) 82
EagI CGGCCG 1 cut(s) 82
Eam1104I CTCTTC 3 cut(s) 87, 169, 299
EarI CTCTTC 3 cut(s) 87, 169, 299
Ecl136II GAGCTC 2 cut(s) 50, 229
EclXI CGGCCG 1 cut(s) 82
Eco147I AGGCCT 1 cut(s) 469
Eco24I GRGCYC 2 cut(s) 52, 231
Eco32I GATATC 2 cut(s) 124, 384
Eco52I CGGCCG 1 cut(s) 82
Eco53kI GAGCTC 2 cut(s) 50, 229
EcoICRI GAGCTC 2 cut(s) 50, 229
EcoRV GATATC 2 cut(s) 124, 384
EcoT38I GRGCYC 2 cut(s) 52, 231
FaeI CATG 4 cut(s) 45, 130, 259, 370
FaiI YATR 7 cut(s) 43, 128, 257, 353, 368, 485, 487
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 1 cut(s) 179
FatI CATG 4 cut(s) 41, 126, 255, 366
FauI CCCGC 1 cut(s) 313
FblI GTMKAC 1 cut(s) 290
Fnu4HI GCNGC 4 cut(s) 22, 103, 238, 443
FokI GGATG 1 cut(s) 95
FriOI GRGCYC 2 cut(s) 52, 231
Fsp4HI GCNGC 4 cut(s) 22, 103, 238, 443
GlaI GCGC 2 cut(s) 115, 236
GluI GCNGC 4 cut(s) 22, 103, 238, 443
GsuI CTGGAG 1 cut(s) 215
HaeIII GGCC 2 cut(s) 84, 469
HgaI GACGC 2 cut(s) 230, 334
HhaI GCGC 2 cut(s) 116, 237
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 4 cut(s) 45, 130, 259, 370
Hin6I GCGC 2 cut(s) 114, 235
HinP1I GCGC 2 cut(s) 114, 235
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HinfI GANTC 2 cut(s) 66, 491
HphI GGTGA 2 cut(s) 128, 486
Hpy166II GTNNAC 2 cut(s) 73, 291
Hpy188I TCNGA 7 cut(s) 17, 65, 178, 271, 381, 388, 415
Hpy188III TCNNGA 5 cut(s) 53, 127, 154, 205, 218
Hpy8I GTNNAC 2 cut(s) 73, 291
Hpy99I CGWCG 5 cut(s) 59, 224, 283, 286, 350
HpyAV CCTTC 2 cut(s) 276, 386
HpyCH4III ACNGT 2 cut(s) 359, 411
HpyCH4IV ACGT 3 cut(s) 57, 147, 451
HpyCH4V TGCA 3 cut(s) 24, 33, 78
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 111, 342
HpyF3I CTNAG 1 cut(s) 475
HpySE526I ACGT 3 cut(s) 57, 147, 451
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 4 cut(s) 45, 130, 259, 370
HspAI GCGC 2 cut(s) 114, 235
Kzo9I GATC 7 cut(s) 60, 96, 178, 201, 207, 388, 436
LmnI GCTCC 4 cut(s) 47, 110, 234, 341
LpnPI CCDG 4 cut(s) 130, 245, 273, 307
Lsp1109I GCAGC 1 cut(s) 8
LweI GCATC 2 cut(s) 8, 33
MaeII ACGT 3 cut(s) 57, 147, 451
MaeIII GTNAC 1 cut(s) 492
MalI GATC 7 cut(s) 62, 98, 180, 203, 209, 390, 438
MboI GATC 7 cut(s) 60, 96, 178, 201, 207, 388, 436
MboII GAAGA 6 cut(s) 16, 104, 156, 286, 313, 316
MhlI GDGCHC 2 cut(s) 52, 231
MlyI GAGTC 2 cut(s) 75, 500
MmeI TCCRAC 2 cut(s) 363, 393
MslI CAYNNNNRTG 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 102
Mva1269I GAATGC 1 cut(s) 33
MvnI CGCG 2 cut(s) 114, 237
MwoI GCNNNNNNNGC 3 cut(s) 30, 111, 342
NdeII GATC 7 cut(s) 60, 96, 178, 201, 207, 388, 436
NlaIII CATG 4 cut(s) 45, 130, 259, 370
NlaIV GGNNCC 1 cut(s) 106
NmeAIII GCCGAG 2 cut(s) 110, 250
NmuCI GTSAC 1 cut(s) 492
OliI CACNNNNGTG 1 cut(s) 186
PagI TCATGA 1 cut(s) 126
PceI AGGCCT 1 cut(s) 469
PcsI WCGNNNNNNNCGW 3 cut(s) 189, 216, 287
PctI GAATGC 1 cut(s) 33
PflFI GACNNNGTC 1 cut(s) 286
PkrI GCNGC 4 cut(s) 23, 104, 239, 444
PleI GAGTC 2 cut(s) 74, 499
PpsI GAGTC 2 cut(s) 74, 499
Psp124BI GAGCTC 2 cut(s) 52, 231
PspN4I GGNNCC 1 cut(s) 106
PsyI GACNNNGTC 1 cut(s) 286
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 1 cut(s) 186
SacI GAGCTC 2 cut(s) 52, 231
SalI GTCGAC 1 cut(s) 289
SatI GCNGC 4 cut(s) 22, 103, 238, 443
Sau3AI GATC 7 cut(s) 60, 96, 178, 201, 207, 388, 436
ScaI AGTACT 1 cut(s) 395
SchI GAGTC 2 cut(s) 75, 500
SduI GDGCHC 2 cut(s) 52, 231
SetI ASST 9 cut(s) 52, 60, 136, 150, 231, 338, 378, 423, 454
SfaNI GCATC 2 cut(s) 8, 33
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 2 cut(s) 152, 397
SmoI CTYRAG 2 cut(s) 152, 397
SseBI AGGCCT 1 cut(s) 469
SsiI CCGC 7 cut(s) 45, 102, 112, 186, 237, 320, 442
SstI GAGCTC 2 cut(s) 52, 231
StuI AGGCCT 1 cut(s) 469
TaaI ACNGT 2 cut(s) 359, 411
TaiI ACGT 3 cut(s) 60, 150, 454
TaqI TCGA 3 cut(s) 54, 290, 315
TatI WGTACW 1 cut(s) 393
TauI GCSGC 3 cut(s) 105, 240, 445
TscAI CASTG 1 cut(s) 80
TseFI GTSAC 1 cut(s) 492
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 1 cut(s) 492
TspDTI ATGAA 3 cut(s) 17, 143, 368
TspRI CASTG 1 cut(s) 80
Tth111I GACNNNGTC 1 cut(s) 286
XmiI GTMKAC 1 cut(s) 290
ZrmI AGTACT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.