RLG00000031792

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016607
Physical Location & Seq
Reverse (-)
8272050 .. 8272553
504 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031792

Sequence Viewer

Length: 504 bp
ATGAAGAACACAATCCGATGCTGCATCTCTTGCATTCTACCATGCGGAGCTCTCGACGTGATCCGAGTCGTTCACTGCAACGGTCGAGTGGAAGAGATCAGCGGCTCCATCCGCGCCAGCGAGATCATGAAGGCTCACCCCAAGCACGTGCTCAAGAAGCCCTCTTCCTCGCCGTCCGATCACGGCGGCGTCGTCCCCAAGATCGTGATTGTACCTCCCGACGCCGAGCTCCAGCGCGGCAAGATTTACTTCCTCATGCCAGTTTCCGAGAAGACGACGAAGTCGACCAAAGTGAAGAAGAGGAAAGTCGAGGCGGGAAATAACGACAAAGCCAACAGCGTCGTTATGAACAGTATTGCCATGACGAACCTTCTGATATCGGATCAGTACTTGAGTGAGATACTGTCGGAAAAGGTTTCGACGCAGAGGGATCGGCGGCGAGGACGTGTCGGCGTGTGGAGGCCTCATTTAGAAAGTATTTGCGAGTCACCAAGTGAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

168

Amino Acids

18.47

Weight (kDa)

9.53

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 7 - 162 3.8e-37 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016607)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29195
fragaria_vesca FvH4_3g07740
malus_domestica MD05G1300400.v1.1 MD10G1279500.v1.1
prunus_persica Prupe.4G061900_v2.0.a1 Prupe.4G061900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0010861
rosa_laevigata RLG00000031792
rosa_multiflora Rmu_sc0000109.1_g000007
rosa_rugosa Rorug04G0453300
rosa_samantha Rh5CG092700 Rh5DG080400
rosa_wichuraiana Rw0G017930 Rw0G022220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 280
AccI GTMKAC 1 cut(s) 284
AccII CGCG 2 cut(s) 114, 237
AciI CCGC 7 cut(s) 45, 102, 112, 186, 237, 314, 436
AclWI GGATC 3 cut(s) 55, 390, 438
AcvI CACGTG 1 cut(s) 148
AcyI GRCGYC 2 cut(s) 189, 222
AdeI CACNNNGTG 1 cut(s) 494
AfaI GTAC 2 cut(s) 213, 389
AflIII ACRYGT 1 cut(s) 445
AjiI CACGTC 2 cut(s) 58, 446
AluBI AGCT 2 cut(s) 50, 229
AluI AGCT 2 cut(s) 50, 229
Alw21I GWGCWC 3 cut(s) 52, 153, 231
AlwI GGATC 3 cut(s) 55, 390, 438
AoxI GGCC 1 cut(s) 461
ApeKI GCWGC 1 cut(s) 21
AspLEI GCGC 2 cut(s) 116, 237
AsuHPI GGTGA 2 cut(s) 128, 480
BanII GRGCYC 2 cut(s) 52, 231
BbrPI CACGTG 1 cut(s) 148
BbsI GAAGAC 1 cut(s) 278
Bbv12I GWGCWC 3 cut(s) 52, 153, 231
BbvI GCAGC 1 cut(s) 8
BccI CCATC 1 cut(s) 116
BceAI ACGGC 2 cut(s) 157, 199
BcgI CGANNNNNNTGC 2 cut(s) 413, 447
BisI GCNGC 5 cut(s) 22, 103, 187, 238, 437
BlsI GCNGC 5 cut(s) 23, 104, 188, 239, 438
BmcAI AGTACT 1 cut(s) 389
BmgBI CACGTC 2 cut(s) 58, 446
BmiI GGNNCC 1 cut(s) 106
BmsI GCATC 2 cut(s) 8, 33
BpiI GAAGAC 1 cut(s) 278
BpmI CTGGAG 1 cut(s) 215
BpuEI CTTGAG 2 cut(s) 137, 412
BsaAI YACGTR 1 cut(s) 148
BsaHI GRCGYC 2 cut(s) 189, 222
Bse1I ACTGG 1 cut(s) 260
BseGI GGATG 1 cut(s) 108
BseNI ACTGG 1 cut(s) 260
BseXI GCAGC 1 cut(s) 8
Bsh1236I CGCG 2 cut(s) 114, 237
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 1 cut(s) 463
BsiEI CGRYCG 1 cut(s) 85
BsiHKAI GWGCWC 3 cut(s) 52, 153, 231
BslFI GGGAC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 179
BsmI GAATGC 1 cut(s) 33
BsnI GGCC 1 cut(s) 463
Bsp1286I GDGCHC 3 cut(s) 52, 153, 231
Bsp143I GATC 7 cut(s) 60, 96, 123, 178, 201, 382, 430
BspACI CCGC 7 cut(s) 45, 102, 112, 186, 237, 314, 436
BspANI GGCC 1 cut(s) 463
BspFNI CGCG 2 cut(s) 114, 237
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 1 cut(s) 106
BspPI GGATC 3 cut(s) 55, 390, 438
BsrI ACTGG 1 cut(s) 260
BssMI GATC 7 cut(s) 60, 96, 123, 178, 201, 382, 430
BssNI GRCGYC 2 cut(s) 189, 222
Bst4CI ACNGT 3 cut(s) 83, 353, 405
Bst6I CTCTTC 3 cut(s) 87, 169, 293
BstACI GRCGYC 2 cut(s) 189, 222
BstAPI GCANNNNNTGC 1 cut(s) 30
BstBAI YACGTR 1 cut(s) 148
BstC8I GCNNGC 1 cut(s) 118
BstF5I GGATG 1 cut(s) 108
BstFNI CGCG 2 cut(s) 114, 237
BstHHI GCGC 2 cut(s) 116, 237
BstKTI GATC 7 cut(s) 63, 99, 126, 181, 204, 385, 433
BstMBI GATC 7 cut(s) 60, 96, 123, 178, 201, 382, 430
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 3 cut(s) 30, 111, 157
BstUI CGCG 2 cut(s) 114, 237
BstV1I GCAGC 1 cut(s) 8
BstV2I GAAGAC 1 cut(s) 278
BsuRI GGCC 1 cut(s) 463
BtrI CACGTC 2 cut(s) 58, 446
BtsCI GGATG 1 cut(s) 108
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 118
CciI TCATGA 1 cut(s) 126
CfoI GCGC 2 cut(s) 116, 237
CseI GACGC 4 cut(s) 178, 230, 328, 430
Csp6I GTAC 2 cut(s) 212, 388
CviAII CATG 4 cut(s) 42, 127, 256, 361
CviJI RGCY 7 cut(s) 50, 105, 134, 160, 229, 332, 463
CviKI_1 RGCY 7 cut(s) 50, 105, 134, 160, 229, 332, 463
CviQI GTAC 2 cut(s) 212, 388
DpnI GATC 7 cut(s) 62, 98, 125, 180, 203, 384, 432
DpnII GATC 7 cut(s) 60, 96, 123, 178, 201, 382, 430
DraIII CACNNNGTG 1 cut(s) 494
DrdI GACNNNNNNGTC 1 cut(s) 280
DseDI GACNNNNNNGTC 1 cut(s) 280
Eam1104I CTCTTC 3 cut(s) 87, 169, 293
EarI CTCTTC 3 cut(s) 87, 169, 293
Ecl136II GAGCTC 2 cut(s) 50, 229
Eco147I AGGCCT 1 cut(s) 463
Eco24I GRGCYC 2 cut(s) 52, 231
Eco32I GATATC 1 cut(s) 378
Eco53kI GAGCTC 2 cut(s) 50, 229
Eco72I CACGTG 1 cut(s) 148
EcoICRI GAGCTC 2 cut(s) 50, 229
EcoRV GATATC 1 cut(s) 378
EcoT38I GRGCYC 2 cut(s) 52, 231
FaeI CATG 4 cut(s) 45, 130, 259, 364
FaiI YATR 5 cut(s) 43, 128, 257, 347, 362
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 1 cut(s) 179
FatI CATG 4 cut(s) 41, 126, 255, 360
FauI CCCGC 1 cut(s) 307
FblI GTMKAC 1 cut(s) 284
Fnu4HI GCNGC 5 cut(s) 22, 103, 187, 238, 437
FokI GGATG 1 cut(s) 95
FriOI GRGCYC 2 cut(s) 52, 231
Fsp4HI GCNGC 5 cut(s) 22, 103, 187, 238, 437
GlaI GCGC 2 cut(s) 115, 236
GluI GCNGC 5 cut(s) 22, 103, 187, 238, 437
GsuI CTGGAG 1 cut(s) 215
HaeIII GGCC 1 cut(s) 463
HgaI GACGC 4 cut(s) 178, 230, 328, 430
HhaI GCGC 2 cut(s) 116, 237
Hin1I GRCGYC 2 cut(s) 189, 222
Hin1II CATG 4 cut(s) 45, 130, 259, 364
Hin6I GCGC 2 cut(s) 114, 235
HinP1I GCGC 2 cut(s) 114, 235
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HinfI GANTC 2 cut(s) 66, 485
HphI GGTGA 2 cut(s) 128, 480
Hpy166II GTNNAC 2 cut(s) 73, 285
Hpy188I TCNGA 7 cut(s) 17, 65, 178, 268, 375, 382, 409
Hpy188III TCNNGA 5 cut(s) 53, 127, 154, 205, 218
Hpy8I GTNNAC 2 cut(s) 73, 285
Hpy99I CGWCG 6 cut(s) 59, 194, 224, 280, 344, 424
HpyAV CCTTC 2 cut(s) 124, 380
HpyCH4III ACNGT 3 cut(s) 83, 353, 405
HpyCH4IV ACGT 3 cut(s) 57, 147, 445
HpyCH4V TGCA 3 cut(s) 24, 33, 78
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 111, 157
HpySE526I ACGT 3 cut(s) 57, 147, 445
Hsp92I GRCGYC 2 cut(s) 189, 222
Hsp92II CATG 4 cut(s) 45, 130, 259, 364
HspAI GCGC 2 cut(s) 114, 235
Kzo9I GATC 7 cut(s) 60, 96, 123, 178, 201, 382, 430
LmnI GCTCC 3 cut(s) 47, 110, 234
LpnPI CCDG 3 cut(s) 130, 245, 273
Lsp1109I GCAGC 1 cut(s) 8
LweI GCATC 2 cut(s) 8, 33
MaeII ACGT 3 cut(s) 57, 147, 445
MaeIII GTNAC 1 cut(s) 486
MalI GATC 7 cut(s) 62, 98, 125, 180, 203, 384, 432
MboI GATC 7 cut(s) 60, 96, 123, 178, 201, 382, 430
MboII GAAGA 6 cut(s) 16, 104, 156, 283, 307, 310
MhlI GDGCHC 3 cut(s) 52, 153, 231
MlyI GAGTC 2 cut(s) 75, 494
MmeI TCCRAC 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 102
Mva1269I GAATGC 1 cut(s) 33
MvnI CGCG 2 cut(s) 114, 237
MwoI GCNNNNNNNGC 3 cut(s) 30, 111, 157
NdeII GATC 7 cut(s) 60, 96, 123, 178, 201, 382, 430
NlaIII CATG 4 cut(s) 45, 130, 259, 364
NlaIV GGNNCC 1 cut(s) 106
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 1 cut(s) 486
PagI TCATGA 1 cut(s) 126
PceI AGGCCT 1 cut(s) 463
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 281
PctI GAATGC 1 cut(s) 33
PflFI GACNNNGTC 1 cut(s) 280
PkrI GCNGC 5 cut(s) 23, 104, 188, 239, 438
PleI GAGTC 2 cut(s) 74, 493
PmaCI CACGTG 1 cut(s) 148
PmlI CACGTG 1 cut(s) 148
PpsI GAGTC 2 cut(s) 74, 493
Ppu21I YACGTR 1 cut(s) 148
Psp124BI GAGCTC 2 cut(s) 52, 231
PspCI CACGTG 1 cut(s) 148
PspN4I GGNNCC 1 cut(s) 106
PsyI GACNNNGTC 1 cut(s) 280
RsaI GTAC 2 cut(s) 213, 389
RsaNI GTAC 2 cut(s) 212, 388
SacI GAGCTC 2 cut(s) 52, 231
SalI GTCGAC 1 cut(s) 283
SatI GCNGC 5 cut(s) 22, 103, 187, 238, 437
Sau3AI GATC 7 cut(s) 60, 96, 123, 178, 201, 382, 430
ScaI AGTACT 1 cut(s) 389
SchI GAGTC 2 cut(s) 75, 494
SduI GDGCHC 3 cut(s) 52, 153, 231
SetI ASST 8 cut(s) 52, 60, 150, 217, 231, 372, 417, 448
SfaNI GCATC 2 cut(s) 8, 33
SmlI CTYRAG 2 cut(s) 152, 391
SmoI CTYRAG 2 cut(s) 152, 391
SseBI AGGCCT 1 cut(s) 463
SsiI CCGC 7 cut(s) 45, 102, 112, 186, 237, 314, 436
SstI GAGCTC 2 cut(s) 52, 231
StuI AGGCCT 1 cut(s) 463
TaaI ACNGT 3 cut(s) 83, 353, 405
TaiI ACGT 3 cut(s) 60, 150, 448
TaqI TCGA 5 cut(s) 54, 85, 284, 309, 419
TatI WGTACW 1 cut(s) 387
TauI GCSGC 4 cut(s) 105, 189, 240, 439
TscAI CASTG 1 cut(s) 80
TseFI GTSAC 1 cut(s) 486
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 1 cut(s) 486
TspDTI ATGAA 3 cut(s) 17, 143, 362
TspRI CASTG 1 cut(s) 80
Tth111I GACNNNGTC 1 cut(s) 280
XmiI GTMKAC 1 cut(s) 284
ZrmI AGTACT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.