MD10G1279500.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016607
Physical Location & Seq
Forward (+)
37056944 .. 37057480
537 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1279500.v1.1.491

Sequence Viewer

Length: 537 bp
ATGAAAAACACCATCAGGTGCTGCATCTCTTGCATTCTTCCATGTGGAGCTTTGGACGTGATTCGAGTCGTACACTCTAACGGCAGAGTCGAGGAAATCAGCGGCACAATCCGGGCCAGCGAGATCATGAAGGCGTACCCTAAGCACGTCCTCAAGAAGCCCTCCTCGTCGGCGTCCGATCACGACGGGGTCGTTCCCAAGATCGTGATCGTTCCGCCCGACGCGGAACTCCAACGAGGAAAGATTTACTTCCTCATGCCGATGCCTGCCGCTTCGAAGACGTCCGAAAAGAAGGGGAGGACAAGATCGTCGTCGGCGAAGAAGAAGCGAATCAAAGACGCCGAAAACAACAACGTCACCAACAACAACAACGTTGTTTTGAACAGCATCACCATGACCAACCTGTTGATAAATGATCGGTACTTGAGCGAAATACTGTCGGAAAAGCATTCGTCGCAGCGGGATCGGCGGCGAGGACGTGTTGGGGTTTGGAGGCCGCACTTAGAGAGCATTTGTGAGTCACCAAGTGATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

179

Amino Acids

19.83

Weight (kDa)

9.77

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 7 - 173 6e-39 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016607)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29195
fragaria_vesca FvH4_3g07740
malus_domestica MD05G1300400.v1.1 MD10G1279500.v1.1
prunus_persica Prupe.4G061900_v2.0.a1 Prupe.4G061900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0010861
rosa_laevigata RLG00000031792
rosa_multiflora Rmu_sc0000109.1_g000007
rosa_rugosa Rorug04G0453300
rosa_samantha Rh5CG092700 Rh5DG080400
rosa_wichuraiana Rw0G017930 Rw0G022220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 307
AatII GACGTC 1 cut(s) 284
AccII CGCG 1 cut(s) 224
AciI CCGC 7 cut(s) 102, 215, 224, 270, 460, 469, 497
AclI AACGTT 1 cut(s) 372
AclWI GGATC 1 cut(s) 471
AcyI GRCGYC 3 cut(s) 173, 281, 339
AdeI CACNNNGTG 1 cut(s) 527
AfaI GTAC 3 cut(s) 72, 137, 422
AflIII ACRYGT 1 cut(s) 478
AgsI TTSAA 1 cut(s) 382
AjiI CACGTC 3 cut(s) 58, 148, 479
AluBI AGCT 1 cut(s) 50
AluI AGCT 1 cut(s) 50
AlwI GGATC 1 cut(s) 471
AlwNI CAGNNNCTG 1 cut(s) 21
AoxI GGCC 2 cut(s) 114, 494
ApeKI GCWGC 2 cut(s) 21, 457
AspS9I GGNCC 1 cut(s) 114
AsuC2I CCSGG 1 cut(s) 113
AsuHPI GGTGA 3 cut(s) 349, 382, 513
AsuII TTCGAA 1 cut(s) 275
BbsI GAAGAC 1 cut(s) 284
BbvI GCAGC 2 cut(s) 8, 469
BccI CCATC 1 cut(s) 20
BceAI ACGGC 1 cut(s) 97
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BcnI CCSGG 1 cut(s) 113
BisI GCNGC 6 cut(s) 22, 103, 270, 458, 470, 497
BlsI GCNGC 6 cut(s) 23, 104, 271, 459, 471, 498
Bme1390I CCNGG 1 cut(s) 113
BmgBI CACGTC 3 cut(s) 58, 148, 479
BmgT120I GGNCC 1 cut(s) 114
BmrFI CCNGG 1 cut(s) 113
BmsI GCATC 3 cut(s) 33, 252, 396
BpiI GAAGAC 1 cut(s) 284
Bpu10I CCTNAGC 1 cut(s) 141
Bpu14I TTCGAA 1 cut(s) 275
BpuEI CTTGAG 2 cut(s) 137, 445
BpuMI CCSGG 1 cut(s) 113
BsaBI GATNNNNATC 1 cut(s) 206
BsaHI GRCGYC 3 cut(s) 173, 281, 339
Bse8I GATNNNNATC 1 cut(s) 206
BseJI GATNNNNATC 1 cut(s) 206
BseRI GAGGAG 1 cut(s) 154
BseXI GCAGC 2 cut(s) 8, 469
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 2 cut(s) 116, 496
BsiSI CCGG 1 cut(s) 112
BsmI GAATGC 2 cut(s) 33, 448
BsnI GGCC 2 cut(s) 116, 496
Bsp119I TTCGAA 1 cut(s) 275
Bsp143I GATC 7 cut(s) 123, 178, 201, 207, 305, 415, 463
BspACI CCGC 7 cut(s) 102, 215, 224, 270, 460, 469, 497
BspANI GGCC 2 cut(s) 116, 496
BspFNI CGCG 1 cut(s) 224
BspHI TCATGA 1 cut(s) 126
BspPI GGATC 1 cut(s) 471
BspT104I TTCGAA 1 cut(s) 275
BssMI GATC 7 cut(s) 123, 178, 201, 207, 305, 415, 463
BssNI GRCGYC 3 cut(s) 173, 281, 339
Bst4CI ACNGT 1 cut(s) 438
BstACI GRCGYC 3 cut(s) 173, 281, 339
BstAPI GCANNNNNTGC 1 cut(s) 30
BstBI TTCGAA 1 cut(s) 275
BstC8I GCNNGC 2 cut(s) 118, 267
BstDEI CTNAG 2 cut(s) 141, 502
BstFNI CGCG 1 cut(s) 224
BstKTI GATC 7 cut(s) 126, 181, 204, 210, 308, 418, 466
BstMBI GATC 7 cut(s) 123, 178, 201, 207, 305, 415, 463
BstMWI GCNNNNNNNGC 3 cut(s) 30, 454, 466
BstSCI CCNGG 1 cut(s) 111
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 2 cut(s) 8, 469
BstV2I GAAGAC 1 cut(s) 284
BsuRI GGCC 2 cut(s) 116, 496
BtrI CACGTC 3 cut(s) 58, 148, 479
Cac8I GCNNGC 2 cut(s) 118, 267
CaiI CAGNNNCTG 1 cut(s) 21
CciI TCATGA 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 114
CseI GACGC 3 cut(s) 162, 230, 347
Csp6I GTAC 3 cut(s) 71, 136, 421
CviAII CATG 4 cut(s) 42, 127, 256, 394
CviJI RGCY 4 cut(s) 50, 116, 160, 496
CviKI_1 RGCY 4 cut(s) 50, 116, 160, 496
CviQI GTAC 3 cut(s) 71, 136, 421
DdeI CTNAG 2 cut(s) 141, 502
DpnI GATC 7 cut(s) 125, 180, 203, 209, 307, 417, 465
DpnII GATC 7 cut(s) 123, 178, 201, 207, 305, 415, 463
DraIII CACNNNGTG 1 cut(s) 527
DrdI GACNNNNNNGTC 1 cut(s) 307
DseDI GACNNNNNNGTC 1 cut(s) 307
EciI GGCGGA 1 cut(s) 204
FaeI CATG 4 cut(s) 45, 130, 259, 397
FaiI YATR 4 cut(s) 43, 128, 257, 395
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FatI CATG 4 cut(s) 41, 126, 255, 393
FauI CCCGC 1 cut(s) 453
Fnu4HI GCNGC 6 cut(s) 22, 103, 270, 458, 470, 497
Fsp4HI GCNGC 6 cut(s) 22, 103, 270, 458, 470, 497
GluI GCNGC 6 cut(s) 22, 103, 270, 458, 470, 497
HaeIII GGCC 2 cut(s) 116, 496
HapII CCGG 1 cut(s) 112
HgaI GACGC 3 cut(s) 162, 230, 347
Hin1I GRCGYC 3 cut(s) 173, 281, 339
Hin1II CATG 4 cut(s) 45, 130, 259, 397
HinfI GANTC 5 cut(s) 61, 66, 87, 330, 518
HpaII CCGG 1 cut(s) 112
HphI GGTGA 3 cut(s) 349, 382, 513
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 3 cut(s) 178, 286, 442
Hpy188III TCNNGA 4 cut(s) 127, 154, 182, 205
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 6 cut(s) 172, 188, 224, 313, 316, 457
HpyAV CCTTC 2 cut(s) 124, 286
HpyCH4III ACNGT 1 cut(s) 438
HpyCH4IV ACGT 6 cut(s) 57, 147, 281, 354, 372, 478
HpyCH4V TGCA 2 cut(s) 24, 33
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 454, 466
HpyF3I CTNAG 2 cut(s) 141, 502
HpySE526I ACGT 6 cut(s) 57, 147, 281, 354, 372, 478
Hsp92I GRCGYC 3 cut(s) 173, 281, 339
Hsp92II CATG 4 cut(s) 45, 130, 259, 397
Kzo9I GATC 7 cut(s) 123, 178, 201, 207, 305, 415, 463
LmnI GCTCC 1 cut(s) 47
LpnPI CCDG 4 cut(s) 125, 130, 279, 416
Lsp1109I GCAGC 2 cut(s) 8, 469
LweI GCATC 3 cut(s) 33, 252, 396
MaeII ACGT 6 cut(s) 57, 147, 281, 354, 372, 478
MaeIII GTNAC 2 cut(s) 355, 519
MalI GATC 7 cut(s) 125, 180, 203, 209, 307, 417, 465
MboI GATC 7 cut(s) 123, 178, 201, 207, 305, 415, 463
MboII GAAGA 4 cut(s) 29, 289, 331, 334
MlyI GAGTC 3 cut(s) 75, 96, 527
MmeI TCCRAC 2 cut(s) 256, 420
MnlI CCTC 9 cut(s) 85, 161, 172, 175, 230, 263, 291, 467, 486
MslI CAYNNNNRTG 2 cut(s) 260, 392
MspA1I CMGCKG 2 cut(s) 102, 460
MspI CCGG 1 cut(s) 112
MspR9I CCNGG 1 cut(s) 113
Mva1269I GAATGC 2 cut(s) 33, 448
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 3 cut(s) 30, 454, 466
NciI CCSGG 1 cut(s) 113
NdeII GATC 7 cut(s) 123, 178, 201, 207, 305, 415, 463
NlaIII CATG 4 cut(s) 45, 130, 259, 397
NmuCI GTSAC 2 cut(s) 355, 519
NspV TTCGAA 1 cut(s) 275
PagI TCATGA 1 cut(s) 126
PcsI WCGNNNNNNNCGW 4 cut(s) 87, 189, 216, 314
PctI GAATGC 2 cut(s) 33, 448
PfeI GAWTC 2 cut(s) 61, 330
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 6 cut(s) 23, 104, 271, 459, 471, 498
PleI GAGTC 3 cut(s) 74, 95, 526
PpsI GAGTC 3 cut(s) 74, 95, 526
Psp1406I AACGTT 1 cut(s) 372
PspPI GGNCC 1 cut(s) 114
PstNI CAGNNNCTG 1 cut(s) 21
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 3 cut(s) 72, 137, 422
RsaNI GTAC 3 cut(s) 71, 136, 421
RseI CAYNNNNRTG 2 cut(s) 260, 392
SatI GCNGC 6 cut(s) 22, 103, 270, 458, 470, 497
Sau3AI GATC 7 cut(s) 123, 178, 201, 207, 305, 415, 463
Sau96I GGNCC 1 cut(s) 114
SchI GAGTC 3 cut(s) 75, 96, 527
ScrFI CCNGG 1 cut(s) 113
SetI ASST 9 cut(s) 20, 52, 60, 150, 284, 357, 375, 405, 481
SfaNI GCATC 3 cut(s) 33, 252, 396
SfuI TTCGAA 1 cut(s) 275
SmiMI CAYNNNNRTG 2 cut(s) 260, 392
SmlI CTYRAG 2 cut(s) 152, 424
SmoI CTYRAG 2 cut(s) 152, 424
SsiI CCGC 7 cut(s) 102, 215, 224, 270, 460, 469, 497
StyD4I CCNGG 1 cut(s) 111
TaaI ACNGT 1 cut(s) 438
TaiI ACGT 6 cut(s) 60, 150, 284, 357, 375, 481
TaqI TCGA 3 cut(s) 64, 90, 275
TauI GCSGC 4 cut(s) 105, 272, 472, 499
TfiI GAWTC 2 cut(s) 61, 330
TseFI GTSAC 2 cut(s) 355, 519
TseI GCWGC 2 cut(s) 21, 457
Tsp45I GTSAC 2 cut(s) 355, 519
TspDTI ATGAA 2 cut(s) 17, 143
Tth111I GACNNNGTC 1 cut(s) 188
ZraI GACGTC 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.