AT1G30350

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009756
Physical Location & Seq
Reverse (-)
10709999 .. 10711667
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G30350.1

Sequence Viewer

Length: 1107 bp
ATGGCTTCTCTCGTGGTAATAGTTTCACTTCTCTTGGCAGCTTTCGCTTCTCCTCTTTTAGAGACTGCTCATAGTTATAATGTAACCGCCCCCAGGGTATCACTAAATCCAATTGATGCTTGCTGGCGAAGAAACCCTAAATGGGCGACTAATCGTCAAGCCCTAGCACATTGCGCGGTAGGGTACGGGAAAGCAGCAATAGGGGGAAAAAACGGTCCGATCTACGTGGTTACAAACCCATCAGACAACCCTACACGCCCTAGCCCCGGAACCTTAAGATATGCGGTGAGCCAACCTAAACCGCTGTGGATCACATTTGCACGTGACATGGTGATAGTGTTGAAAAGCCAGCTCATGATTAACAGCTACAAGACAATCGACGGAAGAGGGGCAAAGGTTGAAATTGCCAACGGACCATGCTTAAGGATCAGACAAGTGAAGCATGTCATTATACATGGGATAAGTATCCATGATTGTAAAGCAGATCCCAATGGAATGGATGGTGATGGAATACGCGTTTTTCAATCTACACATGTTTGGATCGATCATTGTTTCCTTTCTCGGTGCCATGATGGTTTGATCGATGTGATTGTTTCTTCTACGGCTGTCACGATTTCAAATAACTACTTCACGCAGCATGATAAAGTGATGTTACTCGGACATGATGACAGTTATATGGGAGATAAGGACATGAGAGTTACAATTGCATTCAATACTTTTGGACCGGGTCTCATTGAGAGAATGCCTAGGGTGAGGAGAGGGTATGCACATGTAGCAAACAATAGGTACGAGAAATGGCAAATGTACGCGATCGGAGGAAGTGCTAATCCAATTATCTTTAGTGAAGGCAACTACTTCGTTGCTCCCGAAAAACGTAGCAGCAAACAGGTGACGAAGAGAATGATGGCTGGACCTGATTCTAAAAGATGGAAATGGGGTACGTCTAGAGATGTTTTCATGAACGGAGCTTTCTTCGGACCGCCAGGAGTAATAGTCAGGCCGTTGTACAAAGGAGGAGAGGGGTTTCGGGTGGCACATGGCTCTCTCGTCCCTTCTCTTACTTCCTCTGCCGGTCCTCTCCGTTGCTACGTCGGCAGGATTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

368

Amino Acids

40.58

Weight (kDa)

9.75

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 115 - 300 3.3e-28 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009756)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11920 AT1G30350 AT4G22080 AT4G22090
fragaria_vesca FvH4_3g01680
malus_domestica MD00G1202500.v1.1 MD05G1350000.v1.1 MD10G1324800.v1.1
prunus_persica Prupe.4G017400_v2.0.a1 Prupe.4G020600_v2.0.a1
pyrus_communis pycom10g27510
rosa_chinensis RchiOBHm_Chr5g0002531
rosa_laevigata RLG00000031037
rosa_multiflora Rmu_sc0010731.1_g000004
rosa_roxburghii Rroxscaffold_1G00073370
rosa_rugosa Rorug04G0396700
rosa_samantha Rh5AG020100 Rh5BG022800 Rh5CG022100 Rh5DG021200
rosa_wichuraiana Rw5G001850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 78
AccB1I GGYRCC 1 cut(s) 564
AccII CGCG 3 cut(s) 176, 516, 809
AciI CCGC 5 cut(s) 87, 176, 284, 302, 980
AclWI GGATC 4 cut(s) 317, 434, 479, 548
AcvI CACGTG 1 cut(s) 323
AfaI GTAC 5 cut(s) 185, 788, 806, 940, 1007
AfiI CCNNNNNNNGG 3 cut(s) 93, 142, 266
AflII CTTAAG 2 cut(s) 274, 421
AflIII ACRYGT 3 cut(s) 514, 532, 769
AgsI TTSAA 5 cut(s) 343, 401, 524, 618, 712
AhdI GACNNNNNGTC 1 cut(s) 153
AjnI CCWGG 2 cut(s) 92, 982
AluBI AGCT 4 cut(s) 41, 352, 366, 968
AluI AGCT 4 cut(s) 41, 352, 366, 968
Alw26I GTCTC 2 cut(s) 56, 734
AlwI GGATC 4 cut(s) 317, 434, 479, 548
AoxI GGCC 1 cut(s) 998
ApeKI GCWGC 4 cut(s) 38, 194, 634, 879
AspA2I CCTAGG 1 cut(s) 746
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 6 cut(s) 215, 413, 722, 911, 977, 1073
AsuC2I CCSGG 2 cut(s) 267, 726
AsuHPI GGTGA 5 cut(s) 298, 343, 515, 763, 901
AvaII GGWCC 6 cut(s) 215, 413, 722, 911, 977, 1073
AvrII CCTAGG 1 cut(s) 746
BanI GGYRCC 1 cut(s) 564
BauI CACGAG 1 cut(s) 11
BbrPI CACGTG 1 cut(s) 323
BbvI GCAGC 4 cut(s) 50, 206, 646, 891
BccI CCATC 6 cut(s) 247, 494, 500, 566, 898, 921
BceAI ACGGC 2 cut(s) 618, 985
BcgI CGANNNNNNTGC 2 cut(s) 838, 872
BciT130I CCWGG 2 cut(s) 94, 984
BciVI GTATCC 1 cut(s) 476
BcnI CCSGG 2 cut(s) 267, 726
BcoDI GTCTC 2 cut(s) 56, 734
BfaI CTAG 4 cut(s) 164, 261, 747, 945
BfrI CTTAAG 2 cut(s) 274, 421
BfuI GTATCC 1 cut(s) 476
BisI GCNGC 4 cut(s) 39, 195, 635, 880
BlnI CCTAGG 1 cut(s) 746
BlsI GCNGC 4 cut(s) 40, 196, 636, 881
Bme1390I CCNGG 4 cut(s) 94, 267, 726, 984
Bme18I GGWCC 6 cut(s) 215, 413, 722, 911, 977, 1073
BmeRI GACNNNNNGTC 1 cut(s) 153
BmgT120I GGNCC 6 cut(s) 215, 413, 722, 911, 977, 1073
BmiI GGNNCC 2 cut(s) 271, 566
BmrFI CCNGG 4 cut(s) 94, 267, 726, 984
BmsI GCATC 1 cut(s) 106
BpuMI CCSGG 2 cut(s) 267, 726
Bsa29I ATCGAT 2 cut(s) 543, 582
BsaAI YACGTR 2 cut(s) 226, 323
BsaBI GATNNNNATC 1 cut(s) 464
BsaI GGTCTC 1 cut(s) 734
BsaJI CCNNGG 4 cut(s) 92, 93, 265, 746
BsaXI ACNNNNNCTCC 2 cut(s) 978, 1008
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 142, 266
Bse118I RCCGGY 1 cut(s) 1070
Bse3DI GCAATG 1 cut(s) 169
Bse8I GATNNNNATC 1 cut(s) 464
BseBI CCWGG 2 cut(s) 94, 984
BseCI ATCGAT 2 cut(s) 543, 582
BseDI CCNNGG 4 cut(s) 92, 93, 265, 746
BseGI GGATG 1 cut(s) 505
BseJI GATNNNNATC 1 cut(s) 464
BseLI CCNNNNNNNGG 3 cut(s) 93, 142, 266
BseMI GCAATG 1 cut(s) 169
BseRI GAGGAG 3 cut(s) 42, 769, 1029
BseXI GCAGC 4 cut(s) 50, 206, 646, 891
Bsh1236I CGCG 3 cut(s) 176, 516, 809
Bsh1285I CGRYCG 1 cut(s) 813
BshFI GGCC 1 cut(s) 1000
BshNI GGYRCC 1 cut(s) 564
BshVI ATCGAT 2 cut(s) 543, 582
BsiEI CGRYCG 1 cut(s) 813
BsiSI CCGG 3 cut(s) 267, 725, 1071
BslFI GGGAC 1 cut(s) 1034
BslI CCNNNNNNNGG 3 cut(s) 93, 142, 266
BsmAI GTCTC 2 cut(s) 56, 734
BsmFI GGGAC 1 cut(s) 1034
BsmI GAATGC 2 cut(s) 707, 747
BsnI GGCC 1 cut(s) 1000
Bso31I GGTCTC 1 cut(s) 734
Bsp1407I TGTACA 1 cut(s) 1005
Bsp143I GATC 8 cut(s) 219, 309, 426, 484, 540, 544, 579, 810
BspACI CCGC 5 cut(s) 87, 176, 284, 302, 980
BspANI GGCC 1 cut(s) 1000
BspDI ATCGAT 2 cut(s) 543, 582
BspFNI CGCG 3 cut(s) 176, 516, 809
BspHI TCATGA 2 cut(s) 354, 957
BspLI GGNNCC 2 cut(s) 271, 566
BspPI GGATC 4 cut(s) 317, 434, 479, 548
BspT107I GGYRCC 1 cut(s) 564
BspTI CTTAAG 2 cut(s) 274, 421
BspTNI GGTCTC 1 cut(s) 734
BsrDI GCAATG 1 cut(s) 169
BsrFI RCCGGY 1 cut(s) 1070
BsrGI TGTACA 1 cut(s) 1005
BssAI RCCGGY 1 cut(s) 1070
BssECI CCNNGG 4 cut(s) 92, 93, 265, 746
BssMI GATC 8 cut(s) 219, 309, 426, 484, 540, 544, 579, 810
BssSI CACGAG 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 746
Bst2BI CACGAG 1 cut(s) 11
Bst2UI CCWGG 2 cut(s) 94, 984
Bst4CI ACNGT 2 cut(s) 215, 671
Bst6I CTCTTC 2 cut(s) 379, 890
BstAFI CTTAAG 2 cut(s) 274, 421
BstAPI GCANNNNNTGC 1 cut(s) 1101
BstAUI TGTACA 1 cut(s) 1005
BstBAI YACGTR 2 cut(s) 226, 323
BstC8I GCNNGC 3 cut(s) 121, 125, 350
BstF5I GGATG 1 cut(s) 505
BstFNI CGCG 3 cut(s) 176, 516, 809
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 8 cut(s) 222, 312, 429, 487, 543, 547, 582, 813
BstMAI GTCTC 2 cut(s) 56, 734
BstMBI GATC 8 cut(s) 219, 309, 426, 484, 540, 544, 579, 810
BstMCI CGRYCG 1 cut(s) 813
BstMWI GCNNNNNNNGC 5 cut(s) 44, 173, 773, 1092, 1101
BstNI CCWGG 2 cut(s) 94, 984
BstNSI RCATGY 3 cut(s) 446, 536, 773
BstSCI CCNGG 4 cut(s) 92, 265, 724, 982
BstUI CGCG 3 cut(s) 176, 516, 809
BstV1I GCAGC 4 cut(s) 50, 206, 646, 891
BstX2I RGATCY 1 cut(s) 484
BstXI CCANNNNNNTGG 1 cut(s) 496
BstYI RGATCY 1 cut(s) 484
Bsu15I ATCGAT 2 cut(s) 543, 582
BsuI GTATCC 1 cut(s) 476
BsuRI GGCC 1 cut(s) 1000
BsuTUI ATCGAT 2 cut(s) 543, 582
BtsCI GGATG 1 cut(s) 505
Cac8I GCNNGC 3 cut(s) 121, 125, 350
CciI TCATGA 2 cut(s) 354, 957
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 1 cut(s) 1070
Cfr13I GGNCC 6 cut(s) 215, 413, 722, 911, 977, 1073
ClaI ATCGAT 2 cut(s) 543, 582
CpoI CGGWCCG 2 cut(s) 215, 977
Csp6I GTAC 5 cut(s) 184, 787, 805, 939, 1006
CspI CGGWCCG 2 cut(s) 215, 977
CviQI GTAC 5 cut(s) 184, 787, 805, 939, 1006
DpnI GATC 8 cut(s) 221, 311, 428, 486, 542, 546, 581, 812
DpnII GATC 8 cut(s) 219, 309, 426, 484, 540, 544, 579, 810
DriI GACNNNNNGTC 1 cut(s) 153
Eam1104I CTCTTC 2 cut(s) 379, 890
Eam1105I GACNNNNNGTC 1 cut(s) 153
EarI CTCTTC 2 cut(s) 379, 890
Eco130I CCWWGG 1 cut(s) 746
Eco31I GGTCTC 1 cut(s) 734
Eco47I GGWCC 6 cut(s) 215, 413, 722, 911, 977, 1073
Eco72I CACGTG 1 cut(s) 323
EcoRII CCWGG 2 cut(s) 92, 982
EcoT14I CCWWGG 1 cut(s) 746
ErhI CCWWGG 1 cut(s) 746
FaqI GGGAC 1 cut(s) 1034
Fnu4HI GCNGC 4 cut(s) 39, 195, 635, 880
FokI GGATG 1 cut(s) 512
Fsp4HI GCNGC 4 cut(s) 39, 195, 635, 880
FspBI CTAG 4 cut(s) 164, 261, 747, 945
GlaI GCGC 1 cut(s) 175
GluI GCNGC 4 cut(s) 39, 195, 635, 880
HaeIII GGCC 1 cut(s) 1000
HapII CCGG 3 cut(s) 267, 725, 1071
HhaI GCGC 1 cut(s) 176
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HinfI GANTC 1 cut(s) 917
HpaII CCGG 3 cut(s) 267, 725, 1071
HphI GGTGA 5 cut(s) 298, 343, 515, 763, 901
Hpy188I TCNGA 6 cut(s) 219, 244, 431, 659, 815, 977
Hpy188III TCNNGA 5 cut(s) 355, 610, 866, 945, 958
Hpy99I CGWCG 2 cut(s) 383, 1094
HpyAV CCTTC 2 cut(s) 839, 1062
HpyCH4III ACNGT 2 cut(s) 215, 671
HpyCH4IV ACGT 5 cut(s) 225, 322, 874, 941, 1089
HpyCH4V TGCA 3 cut(s) 320, 707, 767
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 173, 773, 1092, 1101
HpySE526I ACGT 5 cut(s) 225, 322, 874, 941, 1089
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 8 cut(s) 219, 309, 426, 484, 540, 544, 579, 810
LmnI GCTCC 2 cut(s) 868, 965
Lsp1109I GCAGC 4 cut(s) 50, 206, 646, 891
LweI GCATC 1 cut(s) 106
MaeI CTAG 4 cut(s) 164, 261, 747, 945
MaeII ACGT 5 cut(s) 225, 322, 874, 941, 1089
MaeIII GTNAC 7 cut(s) 82, 229, 323, 607, 651, 697, 889
MalI GATC 8 cut(s) 221, 311, 428, 486, 542, 546, 581, 812
MboI GATC 8 cut(s) 219, 309, 426, 484, 540, 544, 579, 810
MboII GAAGA 5 cut(s) 141, 396, 588, 907, 964
MfeI CAATTG 2 cut(s) 111, 702
MflI RGATCY 1 cut(s) 484
MluCI AATT 4 cut(s) 111, 402, 702, 831
MluI ACGCGT 1 cut(s) 514
MnlI CCTC 9 cut(s) 63, 380, 747, 752, 809, 1007, 1012, 1075, 1086
MseI TTAA 3 cut(s) 275, 360, 422
MspA1I CMGCKG 1 cut(s) 304
MspCI CTTAAG 2 cut(s) 274, 421
MspI CCGG 3 cut(s) 267, 725, 1071
MspR9I CCNGG 4 cut(s) 94, 267, 726, 984
MunI CAATTG 2 cut(s) 111, 702
Mva1269I GAATGC 2 cut(s) 707, 747
MvaI CCWGG 2 cut(s) 94, 984
MvnI CGCG 3 cut(s) 176, 516, 809
MwoI GCNNNNNNNGC 5 cut(s) 44, 173, 773, 1092, 1101
NciI CCSGG 2 cut(s) 267, 726
NdeII GATC 8 cut(s) 219, 309, 426, 484, 540, 544, 579, 810
NlaIV GGNNCC 2 cut(s) 271, 566
NmuCI GTSAC 3 cut(s) 323, 607, 889
NspI RCATGY 3 cut(s) 446, 536, 773
PagI TCATGA 2 cut(s) 354, 957
PasI CCCWGGG 1 cut(s) 93
PciI ACATGT 2 cut(s) 532, 769
PcsI WCGNNNNNNNCGW 2 cut(s) 608, 864
PctI GAATGC 2 cut(s) 707, 747
PfeI GAWTC 1 cut(s) 917
PflFI GACNNNGTC 1 cut(s) 726
PkrI GCNGC 4 cut(s) 40, 196, 636, 881
Ple19I CGATCG 1 cut(s) 813
PmaCI CACGTG 1 cut(s) 323
PmlI CACGTG 1 cut(s) 323
Ppu21I YACGTR 2 cut(s) 226, 323
PscI ACATGT 2 cut(s) 532, 769
PsiI TTATAA 1 cut(s) 78
Psp6I CCWGG 2 cut(s) 92, 982
PspCI CACGTG 1 cut(s) 323
PspGI CCWGG 2 cut(s) 92, 982
PspN4I GGNNCC 2 cut(s) 271, 566
PspPI GGNCC 6 cut(s) 215, 413, 722, 911, 977, 1073
PsuI RGATCY 1 cut(s) 484
PsyI GACNNNGTC 1 cut(s) 726
PvuI CGATCG 1 cut(s) 813
RsaI GTAC 5 cut(s) 185, 788, 806, 940, 1007
RsaNI GTAC 5 cut(s) 184, 787, 805, 939, 1006
Rsr2I CGGWCCG 2 cut(s) 215, 977
RsrII CGGWCCG 2 cut(s) 215, 977
SaqAI TTAA 3 cut(s) 275, 360, 422
SatI GCNGC 4 cut(s) 39, 195, 635, 880
Sau3AI GATC 8 cut(s) 219, 309, 426, 484, 540, 544, 579, 810
Sau96I GGNCC 6 cut(s) 215, 413, 722, 911, 977, 1073
ScrFI CCNGG 4 cut(s) 94, 267, 726, 984
SfaNI GCATC 1 cut(s) 106
SinI GGWCC 6 cut(s) 215, 413, 722, 911, 977, 1073
SmlI CTYRAG 2 cut(s) 274, 421
SmoI CTYRAG 2 cut(s) 274, 421
Sse9I AATT 4 cut(s) 111, 402, 702, 831
SsiI CCGC 5 cut(s) 87, 176, 284, 302, 980
SspMI CTAG 4 cut(s) 164, 261, 747, 945
StyD4I CCNGG 4 cut(s) 92, 265, 724, 982
StyI CCWWGG 1 cut(s) 746
TaaI ACNGT 2 cut(s) 215, 671
TaiI ACGT 5 cut(s) 228, 325, 877, 944, 1092
TaqI TCGA 3 cut(s) 378, 543, 582
TasI AATT 4 cut(s) 111, 402, 702, 831
TatI WGTACW 1 cut(s) 1005
TfiI GAWTC 1 cut(s) 917
Tru1I TTAA 3 cut(s) 275, 360, 422
Tru9I TTAA 3 cut(s) 275, 360, 422
TseFI GTSAC 3 cut(s) 323, 607, 889
TseI GCWGC 4 cut(s) 38, 194, 634, 879
Tsp45I GTSAC 3 cut(s) 323, 607, 889
TspDTI ATGAA 2 cut(s) 946, 974
TspGWI ACGGA 4 cut(s) 396, 426, 978, 1070
Tth111I GACNNNGTC 1 cut(s) 726
Vha464I CTTAAG 2 cut(s) 274, 421
VpaK11BI GGWCC 6 cut(s) 215, 413, 722, 911, 977, 1073
XbaI TCTAGA 1 cut(s) 944
XceI RCATGY 3 cut(s) 446, 536, 773
XmaJI CCTAGG 1 cut(s) 746
XspI CTAG 4 cut(s) 164, 261, 747, 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.