AT4G22090

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009756
Physical Location & Seq
Reverse (-)
11704003 .. 11706042
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22090.1

Sequence Viewer

Length: 1185 bp
ATGACTCATTTCACCGTTTCGTGCCTCCTCGTGGCACTATTCTTATGTCAGTCCTTGGTCCATGCCGCATATAACGGTTATTACGGGTATAGCCCCGCGGCTGCACCCTACCCCGCCGAAGAACCTCAGAACATCATGAATCCGGTGGACTCCTGTTGGCGGCTCAAGTCTGACTGGGATGTCAATCGTGAAGACCTAGCAGATTGCGCGGTTGGGTTTGGTTCATCTACCTTGGGTGGCAAGAAAGGTAATATATATGTTGTCACAAACCCTTACGACAATGCACAAAACCCCCACCCAGGTTCTTTGCGGTACGGCGTGATCCAAGCCAAGCCATTGTGGATTACTTTCGCTAAGGATATGGTCATAACCCTAGCGAATGAGCTCATGGTCAATAGCTATAAGACCATAGATGGGCGAGGGGCAAAAGTAGAAATTGCATACGGACCATGCATCACGATACAAGACGTGACGAACGTCATTGTGCATGGGATCAGTATCCACGACTGCAAACCAGGGAAATCTGGAAAGGTGAGGAGCAGCCCGACGCATGTCGGTCACCGTAAGGGATCGGACGGTGATGCCATCACTATTTTTGGTTCGTCGAATGTTTGGATCGATCATTGTTACCTAGCGAGTTGTACGGACGGACTCATCGATGTAATCCATGCTTCCACGGCAATCACTATTTCCAACAACTATTTCACTCAGCATGACAAAGTGATGTTGCTCGGACACAACGATAATTTTGTGAAAGATGTGAAAATGAAAGTGACCGTGGCATTCAATCATTTTGGACCAGGACTTGTGGAGAGAATGCCAAGGGTAAGACGAGGCTACGCTCATGTGGCAAACAATAGATACGATAAATGGATAATGTATGCAATTGGTGGTAGCGCCGATCCCACCATTTTCAGTGAAGGCAACTATTTCATTGCTTCTGATAAATCTTACAGCAAAGAGGTGACGAAGAGAGAAGTAAAAGGAGGATGGAACAATTGGAGATGGAGAACCTCAAACGATGTTTTCAAGAACGGAGCTTTCTTCGTACCTTCTGGTTATGGCAGCATTCCGCTACCGTACAGTTCTGCTCAGCGATTCACAGTCGCTCCCGGAAACTTGGTTCCTTCTTTAACCGCAGACGCTGGCCCTCTAAACTGCAACCGCAACGGTCCTTGTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

394

Amino Acids

43.3

Weight (kDa)

8.55

Isoelectric Point (pI)

30.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 128 - 325 2.7e-23 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009756)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11920 AT1G30350 AT4G22080 AT4G22090
fragaria_vesca FvH4_3g01680
malus_domestica MD00G1202500.v1.1 MD05G1350000.v1.1 MD10G1324800.v1.1
prunus_persica Prupe.4G017400_v2.0.a1 Prupe.4G020600_v2.0.a1
pyrus_communis pycom10g27510
rosa_chinensis RchiOBHm_Chr5g0002531
rosa_laevigata RLG00000031037
rosa_multiflora Rmu_sc0010731.1_g000004
rosa_roxburghii Rroxscaffold_1G00073370
rosa_rugosa Rorug04G0396700
rosa_samantha Rh5AG020100 Rh5BG022800 Rh5CG022100 Rh5DG021200
rosa_wichuraiana Rw5G001850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 179
AccII CGCG 2 cut(s) 98, 209
AclWI GGATC 5 cut(s) 316, 500, 577, 623, 896
AfaI GTAC 4 cut(s) 314, 643, 1050, 1082
AfiI CCNNNNNNNGG 4 cut(s) 31, 159, 299, 414
AgsI TTSAA 2 cut(s) 787, 1030
AjiI CACGTC 1 cut(s) 469
AjnI CCWGG 3 cut(s) 298, 514, 799
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
AloI GAACNNNNNNTCC 2 cut(s) 1107, 1139
AluBI AGCT 3 cut(s) 385, 399, 1040
AluI AGCT 3 cut(s) 385, 399, 1040
Alw21I GWGCWC 1 cut(s) 387
AlwI GGATC 5 cut(s) 316, 500, 577, 623, 896
AlwNI CAGNNNCTG 1 cut(s) 1145
AoxI GGCC 1 cut(s) 1147
ApeKI GCWGC 3 cut(s) 101, 540, 1065
AspLEI GCGC 2 cut(s) 209, 899
AspS9I GGNCC 5 cut(s) 58, 446, 797, 1148, 1172
AsuC2I CCSGG 1 cut(s) 1113
AsuHPI GGTGA 5 cut(s) 4, 544, 551, 590, 976
AvaII GGWCC 4 cut(s) 58, 446, 797, 1172
BanII GRGCYC 1 cut(s) 387
BauI CACGAG 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 3 cut(s) 88, 552, 1077
BccI CCATC 4 cut(s) 407, 593, 984, 999
BceAI ACGGC 2 cut(s) 331, 693
BciT130I CCWGG 3 cut(s) 300, 516, 801
BciVI GTATCC 1 cut(s) 509
BcnI CCSGG 1 cut(s) 1113
BfaI CTAG 3 cut(s) 197, 374, 632
BfoI RGCGCY 1 cut(s) 900
BfuI GTATCC 1 cut(s) 509
BisI GCNGC 6 cut(s) 66, 99, 102, 161, 541, 1066
BlpI GCTNAGC 1 cut(s) 1092
BlsI GCNGC 6 cut(s) 67, 100, 103, 162, 542, 1067
Bme1390I CCNGG 4 cut(s) 300, 516, 801, 1113
Bme18I GGWCC 4 cut(s) 58, 446, 797, 1172
BmgBI CACGTC 1 cut(s) 469
BmgT120I GGNCC 5 cut(s) 58, 446, 797, 1148, 1172
BmiI GGNNCC 1 cut(s) 1125
BmrFI CCNGG 4 cut(s) 300, 516, 801, 1113
BmrI ACTGGG 1 cut(s) 184
BmsI GCATC 2 cut(s) 462, 571
BmuI ACTGGG 1 cut(s) 184
BoxI GACNNNNGTC 2 cut(s) 476, 551
BpiI GAAGAC 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 354
Bpu1102I GCTNAGC 1 cut(s) 1092
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 1113
Bsa29I ATCGAT 2 cut(s) 618, 657
BsaBI GATNNNNATC 2 cut(s) 183, 497
BsaJI CCNNGG 8 cut(s) 54, 96, 231, 298, 515, 675, 777, 821
BsaWI WCCGGW 1 cut(s) 142
BsaXI ACNNNNNCTCC 2 cut(s) 1093, 1123
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 159, 299, 414
Bse1I ACTGG 1 cut(s) 179
Bse3DI GCAATG 1 cut(s) 933
Bse8I GATNNNNATC 2 cut(s) 183, 497
BseBI CCWGG 3 cut(s) 300, 516, 801
BseCI ATCGAT 2 cut(s) 618, 657
BseDI CCNNGG 8 cut(s) 54, 96, 231, 298, 515, 675, 777, 821
BseGI GGATG 2 cut(s) 184, 995
BseJI GATNNNNATC 2 cut(s) 183, 497
BseLI CCNNNNNNNGG 4 cut(s) 31, 159, 299, 414
BseMI GCAATG 1 cut(s) 933
BseMII CTCAG 3 cut(s) 140, 722, 1106
BseNI ACTGG 1 cut(s) 179
BseRI GAGGAG 2 cut(s) 17, 550
BseXI GCAGC 3 cut(s) 88, 552, 1077
BsgI GTGCAG 1 cut(s) 87
Bsh1236I CGCG 2 cut(s) 98, 209
BshFI GGCC 1 cut(s) 1149
BshVI ATCGAT 2 cut(s) 618, 657
BsiHKAI GWGCWC 1 cut(s) 387
BsiSI CCGG 2 cut(s) 143, 1113
BslI CCNNNNNNNGG 4 cut(s) 31, 159, 299, 414
BsmI GAATGC 3 cut(s) 782, 822, 1068
BsnI GGCC 1 cut(s) 1149
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 6 cut(s) 321, 492, 569, 615, 619, 901
Bsp1720I GCTNAGC 1 cut(s) 1092
BspANI GGCC 1 cut(s) 1149
BspCNI CTCAG 3 cut(s) 139, 721, 1105
BspDI ATCGAT 2 cut(s) 618, 657
BspFNI CGCG 2 cut(s) 98, 209
BspHI TCATGA 1 cut(s) 135
BspLI GGNNCC 1 cut(s) 1125
BspPI GGATC 5 cut(s) 316, 500, 577, 623, 896
BsrDI GCAATG 1 cut(s) 933
BsrI ACTGG 1 cut(s) 179
BssECI CCNNGG 8 cut(s) 54, 96, 231, 298, 515, 675, 777, 821
BssMI GATC 6 cut(s) 321, 492, 569, 615, 619, 901
BssSI CACGAG 1 cut(s) 29
BssT1I CCWWGG 3 cut(s) 54, 231, 821
Bst2BI CACGAG 1 cut(s) 29
Bst2UI CCWGG 3 cut(s) 300, 516, 801
Bst4CI ACNGT 9 cut(s) 16, 77, 563, 578, 778, 1080, 1085, 1105, 1172
Bst6I CTCTTC 1 cut(s) 965
BstC8I GCNNGC 1 cut(s) 1147
BstDEI CTNAG 4 cut(s) 126, 354, 708, 1092
BstDSI CCRYGG 3 cut(s) 96, 675, 777
BstEII GGTNACC 1 cut(s) 557
BstF5I GGATG 2 cut(s) 184, 995
BstFNI CGCG 2 cut(s) 98, 209
BstH2I RGCGCY 1 cut(s) 900
BstHHI GCGC 2 cut(s) 209, 899
BstKTI GATC 6 cut(s) 324, 495, 572, 618, 622, 904
BstMBI GATC 6 cut(s) 321, 492, 569, 615, 619, 901
BstMWI GCNNNNNNNGC 3 cut(s) 206, 677, 848
BstNI CCWGG 3 cut(s) 300, 516, 801
BstNSI RCATGY 1 cut(s) 554
BstPAI GACNNNNGTC 2 cut(s) 476, 551
BstPI GGTNACC 1 cut(s) 557
BstSCI CCNGG 4 cut(s) 298, 514, 799, 1111
BstUI CGCG 2 cut(s) 98, 209
BstV1I GCAGC 3 cut(s) 88, 552, 1077
BstV2I GAAGAC 1 cut(s) 198
Bsu15I ATCGAT 2 cut(s) 618, 657
BsuI GTATCC 1 cut(s) 509
BsuRI GGCC 1 cut(s) 1149
BsuTUI ATCGAT 2 cut(s) 618, 657
BtgI CCRYGG 3 cut(s) 96, 675, 777
BtrI CACGTC 1 cut(s) 469
BtsCI GGATG 2 cut(s) 184, 995
BtsIMutI CAGTG 1 cut(s) 922
Cac8I GCNNGC 1 cut(s) 1147
CaiI CAGNNNCTG 1 cut(s) 1145
CciI TCATGA 1 cut(s) 135
CfoI GCGC 2 cut(s) 209, 899
Cfr13I GGNCC 5 cut(s) 58, 446, 797, 1148, 1172
Cfr42I CCGCGG 1 cut(s) 99
ClaI ATCGAT 2 cut(s) 618, 657
CseI GACGC 2 cut(s) 556, 1151
Csp6I GTAC 4 cut(s) 313, 642, 1049, 1081
CviAII CATG 9 cut(s) 62, 136, 388, 450, 488, 551, 668, 713, 845
CviQI GTAC 4 cut(s) 313, 642, 1049, 1081
DdeI CTNAG 4 cut(s) 126, 354, 708, 1092
DpnI GATC 6 cut(s) 323, 494, 571, 617, 621, 903
DpnII GATC 6 cut(s) 321, 492, 569, 615, 619, 901
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
Eam1104I CTCTTC 1 cut(s) 965
EarI CTCTTC 1 cut(s) 965
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 3 cut(s) 54, 231, 821
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 4 cut(s) 58, 446, 797, 1172
Eco53kI GAGCTC 1 cut(s) 385
Eco91I GGTNACC 1 cut(s) 557
EcoICRI GAGCTC 1 cut(s) 385
EcoO65I GGTNACC 1 cut(s) 557
EcoRII CCWGG 3 cut(s) 298, 514, 799
EcoT14I CCWWGG 3 cut(s) 54, 231, 821
EcoT22I ATGCAT 1 cut(s) 455
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 3 cut(s) 54, 231, 821
FaeI CATG 9 cut(s) 65, 139, 391, 453, 491, 554, 671, 716, 848
FatI CATG 9 cut(s) 61, 135, 387, 449, 487, 550, 667, 712, 844
FauI CCCGC 2 cut(s) 103, 121
Fnu4HI GCNGC 6 cut(s) 66, 99, 102, 161, 541, 1066
FokI GGATG 2 cut(s) 191, 1002
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 6 cut(s) 66, 99, 102, 161, 541, 1066
FspBI CTAG 3 cut(s) 197, 374, 632
GlaI GCGC 2 cut(s) 208, 898
GluI GCNGC 6 cut(s) 66, 99, 102, 161, 541, 1066
HaeII RGCGCY 1 cut(s) 900
HaeIII GGCC 1 cut(s) 1149
HapII CCGG 2 cut(s) 143, 1113
HgaI GACGC 2 cut(s) 556, 1151
HhaI GCGC 2 cut(s) 209, 899
Hin1II CATG 9 cut(s) 65, 139, 391, 453, 491, 554, 671, 716, 848
Hin6I GCGC 2 cut(s) 207, 897
HinP1I GCGC 2 cut(s) 207, 897
HinfI GANTC 5 cut(s) 4, 139, 149, 651, 1098
HpaII CCGG 2 cut(s) 143, 1113
HphI GGTGA 5 cut(s) 4, 544, 551, 590, 976
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 5 cut(s) 129, 172, 574, 734, 943
Hpy188III TCNNGA 5 cut(s) 136, 188, 457, 525, 1030
Hpy8I GTNNAC 1 cut(s) 148
Hpy99I CGWCG 2 cut(s) 550, 607
HpyAV CCTTC 3 cut(s) 914, 1062, 1137
HpyCH4III ACNGT 9 cut(s) 16, 77, 563, 578, 778, 1080, 1085, 1105, 1172
HpyCH4IV ACGT 2 cut(s) 468, 477
HpyCH4V TGCA 8 cut(s) 104, 284, 440, 453, 487, 510, 884, 1161
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 677, 848
HpyF3I CTNAG 4 cut(s) 126, 354, 708, 1092
HpySE526I ACGT 2 cut(s) 468, 477
Hsp92II CATG 9 cut(s) 65, 139, 391, 453, 491, 554, 671, 716, 848
HspAI GCGC 2 cut(s) 207, 897
KspI CCGCGG 1 cut(s) 99
Kzo9I GATC 6 cut(s) 321, 492, 569, 615, 619, 901
LmnI GCTCC 3 cut(s) 537, 1037, 1114
Lsp1109I GCAGC 3 cut(s) 88, 552, 1077
LweI GCATC 2 cut(s) 462, 571
MaeI CTAG 3 cut(s) 197, 374, 632
MaeII ACGT 2 cut(s) 468, 477
MaeIII GTNAC 6 cut(s) 262, 469, 557, 626, 772, 964
MalI GATC 6 cut(s) 323, 494, 571, 617, 621, 903
MboI GATC 6 cut(s) 321, 492, 569, 615, 619, 901
MboII GAAGA 4 cut(s) 131, 203, 982, 1036
MfeI CAATTG 2 cut(s) 885, 997
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 4 cut(s) 435, 745, 885, 997
MlyI GAGTC 2 cut(s) 143, 645
MmeI TCCRAC 1 cut(s) 717
Mph1103I ATGCAT 1 cut(s) 455
MseI TTAA 1 cut(s) 1133
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 2 cut(s) 143, 1113
MspR9I CCNGG 4 cut(s) 300, 516, 801, 1113
MunI CAATTG 2 cut(s) 885, 997
Mva1269I GAATGC 3 cut(s) 782, 822, 1068
MvaI CCWGG 3 cut(s) 300, 516, 801
MvnI CGCG 2 cut(s) 98, 209
MwoI GCNNNNNNNGC 3 cut(s) 206, 677, 848
NciI CCSGG 1 cut(s) 1113
NdeII GATC 6 cut(s) 321, 492, 569, 615, 619, 901
NlaIII CATG 9 cut(s) 65, 139, 391, 453, 491, 554, 671, 716, 848
NlaIV GGNNCC 1 cut(s) 1125
NmuCI GTSAC 5 cut(s) 262, 469, 557, 772, 964
NsiI ATGCAT 1 cut(s) 455
NspI RCATGY 1 cut(s) 554
PagI TCATGA 1 cut(s) 135
PcsI WCGNNNNNNNCGW 3 cut(s) 474, 654, 738
PctI GAATGC 3 cut(s) 782, 822, 1068
PfeI GAWTC 2 cut(s) 139, 1098
PfoI TCCNGGA 1 cut(s) 1111
PkrI GCNGC 6 cut(s) 67, 100, 103, 162, 542, 1067
PleI GAGTC 2 cut(s) 143, 645
PpsI GAGTC 2 cut(s) 143, 645
PshAI GACNNNNGTC 2 cut(s) 476, 551
Psp124BI GAGCTC 1 cut(s) 387
Psp6I CCWGG 3 cut(s) 298, 514, 799
PspEI GGTNACC 1 cut(s) 557
PspGI CCWGG 3 cut(s) 298, 514, 799
PspN4I GGNNCC 1 cut(s) 1125
PspPI GGNCC 5 cut(s) 58, 446, 797, 1148, 1172
PstNI CAGNNNCTG 1 cut(s) 1145
RsaI GTAC 4 cut(s) 314, 643, 1050, 1082
RsaNI GTAC 4 cut(s) 313, 642, 1049, 1081
SacI GAGCTC 1 cut(s) 387
SacII CCGCGG 1 cut(s) 99
SaqAI TTAA 1 cut(s) 1133
SatI GCNGC 6 cut(s) 66, 99, 102, 161, 541, 1066
Sau3AI GATC 6 cut(s) 321, 492, 569, 615, 619, 901
Sau96I GGNCC 5 cut(s) 58, 446, 797, 1148, 1172
SchI GAGTC 2 cut(s) 143, 645
ScrFI CCNGG 4 cut(s) 300, 516, 801, 1113
SduI GDGCHC 1 cut(s) 387
SfaNI GCATC 2 cut(s) 462, 571
Sfr303I CCGCGG 1 cut(s) 99
SgrBI CCGCGG 1 cut(s) 99
SinI GGWCC 4 cut(s) 58, 446, 797, 1172
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 4 cut(s) 435, 745, 885, 997
SspMI CTAG 3 cut(s) 197, 374, 632
SstI GAGCTC 1 cut(s) 387
StyD4I CCNGG 4 cut(s) 298, 514, 799, 1111
StyI CCWWGG 3 cut(s) 54, 231, 821
TaaI ACNGT 9 cut(s) 16, 77, 563, 578, 778, 1080, 1085, 1105, 1172
TaiI ACGT 2 cut(s) 471, 480
TaqI TCGA 3 cut(s) 605, 618, 657
TaqII GACCGA 1 cut(s) 545
TasI AATT 4 cut(s) 435, 745, 885, 997
TauI GCSGC 3 cut(s) 68, 101, 163
TfiI GAWTC 2 cut(s) 139, 1098
Tru1I TTAA 1 cut(s) 1133
Tru9I TTAA 1 cut(s) 1133
TscAI CASTG 1 cut(s) 922
TseFI GTSAC 5 cut(s) 262, 469, 557, 772, 964
TseI GCWGC 3 cut(s) 101, 540, 1065
Tsp45I GTSAC 5 cut(s) 262, 469, 557, 772, 964
TspDTI ATGAA 4 cut(s) 152, 213, 782, 922
TspGWI ACGGA 4 cut(s) 459, 659, 663, 1050
TspRI CASTG 1 cut(s) 922
VpaK11BI GGWCC 4 cut(s) 58, 446, 797, 1172
XceI RCATGY 1 cut(s) 554
XspI CTAG 3 cut(s) 197, 374, 632
Zsp2I ATGCAT 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.