Prupe.4G020600_v2.0.a1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0009756
Physical Location & Seq
Reverse (-)
964619 .. 965965
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G020600.1

Sequence Viewer

Length: 954 bp
ATGGCCTCCTTATCCCGTCTCATATTGCTCTCATGTTTTCTAACTTACTATATTGCTCCAACATCTCAAGCCTATTCACCGGGCTACTACACCACCACCACCCCAAAAACCACCATAAACGTAGTCGATTTATGTTTGAGCTCCAATTCCAATTGGGCCACTAACCGCCGCACTTTGGCCAACTGGGCAGTAGGCTTTGGCCAAGACGTGGATGACCACGTAAACCCTCAACCTGGCTCACTCCGCTACGGGGTGATCGAAACGCAGCGGCTGTGGATCGTCTTTGCCAAGGATATGGTGATCACACTAAAAAATAAGCTCATTATGAACAGCTTTAAAACCATAGATGGTCGAGGAGCTAAAGTGGAAATTGCCTACGGACCATGCATAACGGTAGGGCACCGGCGAGGATGCGATGGAGATGCCACTTCGCATGTTTGGGTTGACCATTGCTTCCTAGCACGTAGCAGCGATGGCCTCATTGATGTCACCCATGCTTCCACTGCCGTCACCATCACTGACAACTATTTCTCTCAGCATGATAAGGTGATGCTGCTTGGACACAATGATAATTTTAGTGCAGATGAAGGTATGAAAGTCACAATAGCCTTTAACCGTTTTGGGGCTGGCCTGATTGAAAGGATGCCACGATATGACGAGTGGAAGATGTACGCGGTTGGAGGCAGCGCTAACCCGACCATTTTCAGTGAAGGGAACTACTTCATAGCGCCTGAAACTGCTTACGCAAAACAGGCTACAAAGAGAGAGTCTAGAGGACGTTGGAATAATTGGATATGTTCAAAAATGGTGCTTTTTTTTGTTCAATCTGGATATGGAAGCTGTTACCCACTCTACTCTAAAACTCAGTCGTTTAAGGTCCTAGATGGATCCATGGTTCCTGCTTTAACTTCAAGTGCAGGTCCTCTGCGCTGCTTTGTTGGTAAAGCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

318

Amino Acids

35.15

Weight (kDa)

8.77

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009756)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11920 AT1G30350 AT4G22080 AT4G22090
fragaria_vesca FvH4_3g01680
malus_domestica MD00G1202500.v1.1 MD05G1350000.v1.1 MD10G1324800.v1.1
prunus_persica Prupe.4G017400_v2.0.a1 Prupe.4G020600_v2.0.a1
pyrus_communis pycom10g27510
rosa_chinensis RchiOBHm_Chr5g0002531
rosa_laevigata RLG00000031037
rosa_multiflora Rmu_sc0010731.1_g000004
rosa_roxburghii Rroxscaffold_1G00073370
rosa_rugosa Rorug04G0396700
rosa_samantha Rh5AG020100 Rh5BG022800 Rh5CG022100 Rh5DG021200
rosa_wichuraiana Rw5G001850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 908
AccB1I GGYRCC 1 cut(s) 399
AccB7I CCANNNNNTGG 1 cut(s) 208
AccII CGCG 1 cut(s) 674
AciI CCGC 5 cut(s) 166, 169, 244, 268, 674
AclWI GGATC 3 cut(s) 284, 882, 895
AcoI YGGCCR 2 cut(s) 177, 199
AfaI GTAC 1 cut(s) 671
AfeI AGCGCT 1 cut(s) 688
AfiI CCNNNNNNNGG 5 cut(s) 175, 208, 233, 250, 622
AgsI TTSAA 4 cut(s) 638, 801, 824, 912
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 1 cut(s) 232
AloI GAACNNNNNNTCC 2 cut(s) 879, 911
AluBI AGCT 5 cut(s) 141, 319, 333, 359, 840
AluI AGCT 5 cut(s) 141, 319, 333, 359, 840
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 1 cut(s) 23
AlwI GGATC 3 cut(s) 284, 882, 895
AlwNI CAGNNNCTG 1 cut(s) 271
Aor51HI AGCGCT 1 cut(s) 688
AoxI GGCC 6 cut(s) 3, 156, 177, 199, 475, 628
ApeKI GCWGC 5 cut(s) 265, 468, 553, 684, 930
ArsI GACNNNNNNTTYG 2 cut(s) 752, 784
Asp700I GAANNNNTTC 1 cut(s) 719
AspLEI GCGC 3 cut(s) 689, 730, 930
AspS9I GGNCC 4 cut(s) 156, 380, 877, 920
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 6 cut(s) 69, 265, 310, 481, 502, 559
AvaII GGWCC 3 cut(s) 380, 877, 920
BaeGI GKGCMC 1 cut(s) 402
BalI TGGCCA 2 cut(s) 179, 201
BamHI GGATCC 1 cut(s) 887
BanI GGYRCC 1 cut(s) 399
BanII GRGCYC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 143
BbvI GCAGC 5 cut(s) 277, 480, 540, 696, 917
BccI CCATC 5 cut(s) 341, 410, 467, 521, 878
BceAI ACGGC 1 cut(s) 491
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BciT130I CCWGG 1 cut(s) 234
BclI TGATCA 1 cut(s) 300
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 3 cut(s) 458, 771, 881
BfoI RGCGCY 2 cut(s) 690, 731
BfuAI ACCTGC 1 cut(s) 908
BglI GCCNNNNNGGC 1 cut(s) 185
BisI GCNGC 7 cut(s) 169, 266, 269, 469, 554, 685, 931
BlsI GCNGC 7 cut(s) 170, 267, 270, 470, 555, 686, 932
Bme1390I CCNGG 2 cut(s) 81, 234
Bme18I GGWCC 3 cut(s) 380, 877, 920
BmgBI CACGTC 1 cut(s) 208
BmgT120I GGNCC 4 cut(s) 156, 380, 877, 920
BmiI GGNNCC 3 cut(s) 401, 889, 897
BmrFI CCNGG 2 cut(s) 81, 234
BmrI ACTGGG 1 cut(s) 193
BmsI GCATC 4 cut(s) 401, 412, 540, 633
BmuI ACTGGG 1 cut(s) 193
BpuEI CTTGAG 1 cut(s) 51
BpuMI CCSGG 1 cut(s) 81
BsaAI YACGTR 2 cut(s) 220, 464
BsaJI CCNNGG 2 cut(s) 288, 891
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 5 cut(s) 175, 208, 233, 250, 622
Bse118I RCCGGY 1 cut(s) 402
Bse1I ACTGG 1 cut(s) 188
Bse3DI GCAATG 1 cut(s) 448
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 2 cut(s) 288, 891
BseGI GGATG 3 cut(s) 217, 416, 648
BseLI CCNNNNNNNGG 5 cut(s) 175, 208, 233, 250, 622
BseMI GCAATG 1 cut(s) 448
BseMII CTCAG 2 cut(s) 548, 878
BseNI ACTGG 1 cut(s) 188
BseRI GAGGAG 1 cut(s) 369
BseSI GKGCMC 1 cut(s) 402
BseXI GCAGC 5 cut(s) 277, 480, 540, 696, 917
BsgI GTGCAG 2 cut(s) 600, 936
Bsh1236I CGCG 1 cut(s) 674
BshFI GGCC 6 cut(s) 5, 158, 179, 201, 477, 630
BshNI GGYRCC 1 cut(s) 399
BsiHKAI GWGCWC 1 cut(s) 143
BsiSI CCGG 2 cut(s) 80, 403
BslI CCNNNNNNNGG 5 cut(s) 175, 208, 233, 250, 622
BsmAI GTCTC 1 cut(s) 23
BsmBI CGTCTC 1 cut(s) 23
BsnI GGCC 6 cut(s) 5, 158, 179, 201, 477, 630
Bsp1286I GDGCHC 2 cut(s) 143, 402
Bsp143I GATC 4 cut(s) 255, 276, 300, 887
Bsp19I CCATGG 1 cut(s) 891
BspACI CCGC 5 cut(s) 166, 169, 244, 268, 674
BspANI GGCC 6 cut(s) 5, 158, 179, 201, 477, 630
BspCNI CTCAG 2 cut(s) 547, 877
BspFNI CGCG 1 cut(s) 674
BspLI GGNNCC 3 cut(s) 401, 889, 897
BspMI ACCTGC 1 cut(s) 908
BspPI GGATC 3 cut(s) 284, 882, 895
BspT107I GGYRCC 1 cut(s) 399
BsrDI GCAATG 1 cut(s) 448
BsrFI RCCGGY 1 cut(s) 402
BsrI ACTGG 1 cut(s) 188
BssAI RCCGGY 1 cut(s) 402
BssECI CCNNGG 2 cut(s) 288, 891
BssMI GATC 4 cut(s) 255, 276, 300, 887
BssT1I CCWWGG 2 cut(s) 288, 891
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 2 cut(s) 394, 617
BstBAI YACGTR 2 cut(s) 220, 464
BstC8I GCNNGC 1 cut(s) 628
BstDEI CTNAG 2 cut(s) 534, 864
BstDSI CCRYGG 1 cut(s) 891
BstF5I GGATG 3 cut(s) 217, 416, 648
BstFNI CGCG 1 cut(s) 674
BstH2I RGCGCY 2 cut(s) 690, 731
BstHHI GCGC 3 cut(s) 689, 730, 930
BstKTI GATC 4 cut(s) 258, 279, 303, 890
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 4 cut(s) 255, 276, 300, 887
BstMWI GCNNNNNNNGC 5 cut(s) 185, 243, 474, 503, 752
BstNI CCWGG 1 cut(s) 234
BstNSI RCATGY 2 cut(s) 437, 951
BstSCI CCNGG 2 cut(s) 79, 232
BstSLI GKGCMC 1 cut(s) 402
BstUI CGCG 1 cut(s) 674
BstV1I GCAGC 5 cut(s) 277, 480, 540, 696, 917
BstX2I RGATCY 1 cut(s) 887
BstXI CCANNNNNNTGG 1 cut(s) 295
BstYI RGATCY 1 cut(s) 887
BsuRI GGCC 6 cut(s) 5, 158, 179, 201, 477, 630
BtgI CCRYGG 1 cut(s) 891
BtgZI GCGATG 2 cut(s) 429, 486
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 3 cut(s) 217, 416, 648
BtsI GCAGTG 1 cut(s) 501
BtsIMutI CAGTG 3 cut(s) 501, 516, 712
BveI ACCTGC 1 cut(s) 908
Cac8I GCNNGC 1 cut(s) 628
CaiI CAGNNNCTG 1 cut(s) 271
CfoI GCGC 3 cut(s) 689, 730, 930
Cfr10I RCCGGY 1 cut(s) 402
Cfr13I GGNCC 4 cut(s) 156, 380, 877, 920
Csp6I GTAC 1 cut(s) 670
CviAII CATG 7 cut(s) 33, 384, 434, 494, 539, 892, 948
CviQI GTAC 1 cut(s) 670
DdeI CTNAG 2 cut(s) 534, 864
DpnI GATC 4 cut(s) 257, 278, 302, 889
DpnII GATC 4 cut(s) 255, 276, 300, 887
DraI TTTAAA 1 cut(s) 337
EaeI YGGCCR 2 cut(s) 177, 199
Ecl136II GAGCTC 1 cut(s) 141
Eco130I CCWWGG 2 cut(s) 288, 891
Eco24I GRGCYC 1 cut(s) 143
Eco47I GGWCC 3 cut(s) 380, 877, 920
Eco47III AGCGCT 1 cut(s) 688
Eco53kI GAGCTC 1 cut(s) 141
EcoICRI GAGCTC 1 cut(s) 141
EcoO109I RGGNCCY 2 cut(s) 877, 920
EcoRII CCWGG 1 cut(s) 232
EcoT14I CCWWGG 2 cut(s) 288, 891
EcoT22I ATGCAT 1 cut(s) 389
EcoT38I GRGCYC 1 cut(s) 143
ErhI CCWWGG 2 cut(s) 288, 891
Esp3I CGTCTC 1 cut(s) 23
FaeI CATG 7 cut(s) 36, 387, 437, 497, 542, 895, 951
FatI CATG 7 cut(s) 32, 383, 433, 493, 538, 891, 947
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 7 cut(s) 169, 266, 269, 469, 554, 685, 931
FokI GGATG 3 cut(s) 224, 423, 655
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 7 cut(s) 169, 266, 269, 469, 554, 685, 931
FspBI CTAG 3 cut(s) 458, 771, 881
GlaI GCGC 3 cut(s) 688, 729, 929
GluI GCNGC 7 cut(s) 169, 266, 269, 469, 554, 685, 931
HaeII RGCGCY 2 cut(s) 690, 731
HaeIII GGCC 6 cut(s) 5, 158, 179, 201, 477, 630
HapII CCGG 2 cut(s) 80, 403
HhaI GCGC 3 cut(s) 689, 730, 930
Hin1II CATG 7 cut(s) 36, 387, 437, 497, 542, 895, 951
Hin6I GCGC 3 cut(s) 687, 728, 928
HinP1I GCGC 3 cut(s) 687, 728, 928
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HinfI GANTC 1 cut(s) 767
HpaII CCGG 2 cut(s) 80, 403
HphI GGTGA 6 cut(s) 69, 265, 310, 481, 502, 559
Hpy166II GTNNAC 2 cut(s) 223, 445
Hpy188III TCNNGA 2 cut(s) 771, 828
Hpy8I GTNNAC 2 cut(s) 223, 445
HpyAV CCTTC 2 cut(s) 581, 704
HpyCH4III ACNGT 2 cut(s) 394, 617
HpyCH4IV ACGT 5 cut(s) 120, 207, 219, 463, 778
HpyCH4V TGCA 3 cut(s) 387, 581, 917
HpyF10VI GCNNNNNNNGC 5 cut(s) 185, 243, 474, 503, 752
HpyF3I CTNAG 2 cut(s) 534, 864
HpySE526I ACGT 5 cut(s) 120, 207, 219, 463, 778
Hsp92II CATG 7 cut(s) 36, 387, 437, 497, 542, 895, 951
HspAI GCGC 3 cut(s) 687, 728, 928
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 4 cut(s) 255, 276, 300, 887
LmnI GCTCC 3 cut(s) 61, 146, 356
Lsp1109I GCAGC 5 cut(s) 277, 480, 540, 696, 917
LweI GCATC 4 cut(s) 401, 412, 540, 633
MaeI CTAG 3 cut(s) 458, 771, 881
MaeII ACGT 5 cut(s) 120, 207, 219, 463, 778
MaeIII GTNAC 4 cut(s) 487, 508, 598, 842
MalI GATC 4 cut(s) 257, 278, 302, 889
MboI GATC 4 cut(s) 255, 276, 300, 887
MboII GAAGA 1 cut(s) 676
MfeI CAATTG 1 cut(s) 151
MflI RGATCY 1 cut(s) 887
MhlI GDGCHC 2 cut(s) 143, 402
MlsI TGGCCA 2 cut(s) 179, 201
MluCI AATT 5 cut(s) 145, 151, 369, 571, 787
MluNI TGGCCA 2 cut(s) 179, 201
MlyI GAGTC 1 cut(s) 776
MmeI TCCRAC 3 cut(s) 83, 658, 761
MnlI CCTC 8 cut(s) 16, 237, 347, 401, 488, 674, 767, 933
Mox20I TGGCCA 2 cut(s) 179, 201
Mph1103I ATGCAT 1 cut(s) 389
MroXI GAANNNNTTC 1 cut(s) 719
MscI TGGCCA 2 cut(s) 179, 201
MseI TTAA 5 cut(s) 336, 612, 873, 905, 952
Msp20I TGGCCA 2 cut(s) 179, 201
MspA1I CMGCKG 1 cut(s) 268
MspI CCGG 2 cut(s) 80, 403
MspR9I CCNGG 2 cut(s) 81, 234
MunI CAATTG 1 cut(s) 151
MvaI CCWGG 1 cut(s) 234
MvnI CGCG 1 cut(s) 674
MwoI GCNNNNNNNGC 5 cut(s) 185, 243, 474, 503, 752
NciI CCSGG 1 cut(s) 81
NcoI CCATGG 1 cut(s) 891
NdeII GATC 4 cut(s) 255, 276, 300, 887
NlaIII CATG 7 cut(s) 36, 387, 437, 497, 542, 895, 951
NlaIV GGNNCC 3 cut(s) 401, 889, 897
NmuCI GTSAC 3 cut(s) 487, 508, 598
NsiI ATGCAT 1 cut(s) 389
NspI RCATGY 2 cut(s) 437, 951
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 719
PflMI CCANNNNNTGG 1 cut(s) 208
PkrI GCNGC 7 cut(s) 170, 267, 270, 470, 555, 686, 932
PleI GAGTC 1 cut(s) 775
PpsI GAGTC 1 cut(s) 775
Ppu21I YACGTR 2 cut(s) 220, 464
PpuMI RGGWCCY 2 cut(s) 877, 920
Psp124BI GAGCTC 1 cut(s) 143
Psp5II RGGWCCY 2 cut(s) 877, 920
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 3 cut(s) 401, 889, 897
PspPI GGNCC 4 cut(s) 156, 380, 877, 920
PspPPI RGGWCCY 2 cut(s) 877, 920
PstNI CAGNNNCTG 1 cut(s) 271
PsuI RGATCY 1 cut(s) 887
RsaI GTAC 1 cut(s) 671
RsaNI GTAC 1 cut(s) 670
SacI GAGCTC 1 cut(s) 143
SaqAI TTAA 5 cut(s) 336, 612, 873, 905, 952
SatI GCNGC 7 cut(s) 169, 266, 269, 469, 554, 685, 931
Sau3AI GATC 4 cut(s) 255, 276, 300, 887
Sau96I GGNCC 4 cut(s) 156, 380, 877, 920
SchI GAGTC 1 cut(s) 776
ScrFI CCNGG 2 cut(s) 81, 234
SduI GDGCHC 2 cut(s) 143, 402
SfaNI GCATC 4 cut(s) 401, 412, 540, 633
SgrAI CRCCGGYG 1 cut(s) 402
SinI GGWCC 3 cut(s) 380, 877, 920
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
Sse9I AATT 5 cut(s) 145, 151, 369, 571, 787
SsiI CCGC 5 cut(s) 166, 169, 244, 268, 674
SspMI CTAG 3 cut(s) 458, 771, 881
SstI GAGCTC 1 cut(s) 143
StyD4I CCNGG 2 cut(s) 79, 232
StyI CCWWGG 2 cut(s) 288, 891
TaaI ACNGT 2 cut(s) 394, 617
TaiI ACGT 5 cut(s) 123, 210, 222, 466, 781
TaqI TCGA 3 cut(s) 126, 258, 352
TasI AATT 5 cut(s) 145, 151, 369, 571, 787
TauI GCSGC 2 cut(s) 171, 271
Tru1I TTAA 5 cut(s) 336, 612, 873, 905, 952
Tru9I TTAA 5 cut(s) 336, 612, 873, 905, 952
TscAI CASTG 3 cut(s) 508, 523, 712
TseFI GTSAC 3 cut(s) 487, 508, 598
TseI GCWGC 5 cut(s) 265, 468, 553, 684, 930
Tsp45I GTSAC 3 cut(s) 487, 508, 598
TspDTI ATGAA 4 cut(s) 341, 600, 608, 712
TspGWI ACGGA 1 cut(s) 393
TspRI CASTG 3 cut(s) 508, 523, 712
Van91I CCANNNNNTGG 1 cut(s) 208
VpaK11BI GGWCC 3 cut(s) 380, 877, 920
XbaI TCTAGA 1 cut(s) 770
XceI RCATGY 2 cut(s) 437, 951
XmnI GAANNNNTTC 1 cut(s) 719
XspI CTAG 3 cut(s) 458, 771, 881
Zsp2I ATGCAT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.