Rorug04G0396700

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000301
Physical Location & Seq
Forward (+)
56286076 .. 56288604
2529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0396700.1

Sequence Viewer

Length: 498 bp
ATGTCGGCTACAAACACGGAGGATGTGAAGGACCAAGAGCTTGCTGATAGTGCTCTCAATAACCAAGGTCACGGTGACAGTGCCGAGCAAGACATAAAAGCTTCAGATGAAAGCCGCGATGAACATCCCATGCCTTCAGTTCAGAAGGAGGAGGAAACAATTAAGAAAAAGTATGGGGGTATTATTCCTAGGAAGCCTCCACTAATATCCAAGGACCATGAGCGGGCTTATTTTGATTCTGCTGATTGGGCATTGGGAAAGCAGGGAGCCCATAAGCCGAAAGGACCACTTGAAGCACTCCGCCCAAAGCTGCAGCCTACACCACACCAGCAAGTGCGTTCGAGGCGCGCCTCCTATACGCGCGCAGATGATGTTGAAGTAGATGGCGTCAATAACAACACTTCGGAGGAGGAAGTCCAATCCAGCGAAACCGATGGTAGCAGCAGTAACAACACCAGCACAAACACCGCTACTGAGGATGAGAGCCGCCATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

165

Amino Acids

18.24

Weight (kDa)

4.94

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endosulfine PF04667 45 - 117 1e-25 cAMP-regulated phosphoprotein/endosulfine conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 223
AccII CGCG 4 cut(s) 117, 348, 361, 363
AciI CCGC 5 cut(s) 115, 223, 301, 468, 487
AcuI CTGAAG 2 cut(s) 87, 120
AcyI GRCGYC 1 cut(s) 387
AfiI CCNNNNNNNGG 1 cut(s) 223
AgsI TTSAA 2 cut(s) 293, 377
AluBI AGCT 3 cut(s) 40, 101, 310
AluI AGCT 3 cut(s) 40, 101, 310
Alw21I GWGCWC 1 cut(s) 55
ApeKI GCWGC 3 cut(s) 310, 313, 441
AscI GGCGCGCC 1 cut(s) 346
AspA2I CCTAGG 1 cut(s) 188
AspLEI GCGC 4 cut(s) 348, 350, 363, 365
AspS9I GGNCC 3 cut(s) 31, 214, 284
AsuHPI GGTGA 1 cut(s) 86
AvaII GGWCC 3 cut(s) 31, 214, 284
AvrII CCTAGG 1 cut(s) 188
BanII GRGCYC 1 cut(s) 271
Bbv12I GWGCWC 1 cut(s) 55
BbvI GCAGC 3 cut(s) 297, 325, 453
BccI CCATC 2 cut(s) 377, 428
BfaI CTAG 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 311
BisI GCNGC 5 cut(s) 115, 311, 314, 442, 487
BlnI CCTAGG 1 cut(s) 188
BlsI GCNGC 5 cut(s) 116, 312, 315, 443, 488
Bme18I GGWCC 3 cut(s) 31, 214, 284
BmgT120I GGNCC 3 cut(s) 31, 214, 284
BmiI GGNNCC 1 cut(s) 268
BsaBI GATNNNNATC 1 cut(s) 123
BsaHI GRCGYC 1 cut(s) 387
BsaJI CCNNGG 3 cut(s) 64, 188, 210
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse8I GATNNNNATC 1 cut(s) 123
BseDI CCNNGG 3 cut(s) 64, 188, 210
BseGI GGATG 3 cut(s) 28, 124, 484
BseJI GATNNNNATC 1 cut(s) 123
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMII CTCAG 1 cut(s) 465
BsePI GCGCGC 2 cut(s) 346, 361
BseRI GAGGAG 2 cut(s) 164, 422
BseXI GCAGC 3 cut(s) 297, 325, 453
Bsh1236I CGCG 4 cut(s) 117, 348, 361, 363
BsiHKAI GWGCWC 1 cut(s) 55
BslI CCNNNNNNNGG 1 cut(s) 223
Bsp1286I GDGCHC 2 cut(s) 55, 271
BspACI CCGC 5 cut(s) 115, 223, 301, 468, 487
BspCNI CTCAG 1 cut(s) 466
BspFNI CGCG 4 cut(s) 117, 348, 361, 363
BspLI GGNNCC 1 cut(s) 268
BspMAI CTGCAG 1 cut(s) 315
BsrBI CCGCTC 1 cut(s) 223
BssECI CCNNGG 3 cut(s) 64, 188, 210
BssHII GCGCGC 2 cut(s) 346, 361
BssNI GRCGYC 1 cut(s) 387
BssT1I CCWWGG 3 cut(s) 64, 188, 210
Bst4CI ACNGT 2 cut(s) 74, 80
BstACI GRCGYC 1 cut(s) 387
BstC8I GCNNGC 4 cut(s) 42, 225, 348, 363
BstDEI CTNAG 1 cut(s) 474
BstF5I GGATG 3 cut(s) 28, 124, 484
BstFNI CGCG 4 cut(s) 117, 348, 361, 363
BstHHI GCGC 4 cut(s) 348, 350, 363, 365
BstMWI GCNNNNNNNGC 3 cut(s) 50, 248, 343
BstSFI CTRYAG 1 cut(s) 311
BstUI CGCG 4 cut(s) 117, 348, 361, 363
BstV1I GCAGC 3 cut(s) 297, 325, 453
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 3 cut(s) 28, 124, 484
BtsIMutI CAGTG 1 cut(s) 85
Cac8I GCNNGC 4 cut(s) 42, 225, 348, 363
CfoI GCGC 4 cut(s) 348, 350, 363, 365
Cfr13I GGNCC 3 cut(s) 31, 214, 284
CseI GACGC 1 cut(s) 376
CviAII CATG 3 cut(s) 130, 218, 491
DdeI CTNAG 1 cut(s) 474
EciI GGCGGA 1 cut(s) 290
Eco130I CCWWGG 3 cut(s) 64, 188, 210
Eco24I GRGCYC 1 cut(s) 271
Eco47I GGWCC 3 cut(s) 31, 214, 284
Eco57I CTGAAG 2 cut(s) 87, 120
EcoT14I CCWWGG 3 cut(s) 64, 188, 210
EcoT38I GRGCYC 1 cut(s) 271
ErhI CCWWGG 3 cut(s) 64, 188, 210
FaeI CATG 3 cut(s) 133, 221, 494
FaiI YATR 7 cut(s) 95, 131, 174, 219, 273, 357, 492
FalI AAGNNNNNCTT 2 cut(s) 273, 305
FatI CATG 3 cut(s) 129, 217, 490
FauI CCCGC 1 cut(s) 216
Fnu4HI GCNGC 5 cut(s) 115, 311, 314, 442, 487
FokI GGATG 3 cut(s) 35, 111, 491
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 5 cut(s) 115, 311, 314, 442, 487
FspBI CTAG 1 cut(s) 189
GlaI GCGC 4 cut(s) 347, 349, 362, 364
GluI GCNGC 5 cut(s) 115, 311, 314, 442, 487
HgaI GACGC 1 cut(s) 376
HhaI GCGC 4 cut(s) 348, 350, 363, 365
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 3 cut(s) 133, 221, 494
Hin6I GCGC 4 cut(s) 346, 348, 361, 363
HinP1I GCGC 4 cut(s) 346, 348, 361, 363
HindIII AAGCTT 1 cut(s) 99
HinfI GANTC 1 cut(s) 236
HphI GGTGA 1 cut(s) 86
Hpy188I TCNGA 3 cut(s) 106, 144, 406
HpyAV CCTTC 3 cut(s) 22, 139, 144
HpyCH4III ACNGT 2 cut(s) 74, 80
HpyCH4V TGCA 1 cut(s) 313
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 248, 343
HpyF3I CTNAG 1 cut(s) 474
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 3 cut(s) 133, 221, 494
HspAI GCGC 4 cut(s) 346, 348, 361, 363
LmnI GCTCC 1 cut(s) 266
LpnPI CCDG 4 cut(s) 248, 341, 436, 469
Lsp1109I GCAGC 3 cut(s) 297, 325, 453
MaeI CTAG 1 cut(s) 189
MaeIII GTNAC 3 cut(s) 68, 74, 446
MbiI CCGCTC 1 cut(s) 223
MhlI GDGCHC 2 cut(s) 55, 271
MluCI AATT 1 cut(s) 159
MnlI CCTC 9 cut(s) 13, 142, 145, 207, 336, 361, 400, 403, 469
MseI TTAA 1 cut(s) 162
MvnI CGCG 4 cut(s) 117, 348, 361, 363
MwoI GCNNNNNNNGC 3 cut(s) 50, 248, 343
NlaIII CATG 3 cut(s) 133, 221, 494
NlaIV GGNNCC 1 cut(s) 268
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 2 cut(s) 68, 74
PalAI GGCGCGCC 1 cut(s) 346
PauI GCGCGC 2 cut(s) 346, 361
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 5 cut(s) 116, 312, 315, 443, 488
PspN4I GGNNCC 1 cut(s) 268
PspPI GGNCC 3 cut(s) 31, 214, 284
PstI CTGCAG 1 cut(s) 315
PteI GCGCGC 2 cut(s) 346, 361
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 5 cut(s) 115, 311, 314, 442, 487
Sau96I GGNCC 3 cut(s) 31, 214, 284
SduI GDGCHC 2 cut(s) 55, 271
SetI ASST 4 cut(s) 42, 70, 103, 312
SfcI CTRYAG 1 cut(s) 311
SgsI GGCGCGCC 1 cut(s) 346
SinI GGWCC 3 cut(s) 31, 214, 284
Sse9I AATT 1 cut(s) 159
SsiI CCGC 5 cut(s) 115, 223, 301, 468, 487
SspMI CTAG 1 cut(s) 189
StyI CCWWGG 3 cut(s) 64, 188, 210
TaaI ACNGT 2 cut(s) 74, 80
TaqI TCGA 1 cut(s) 341
TasI AATT 1 cut(s) 159
TauI GCSGC 2 cut(s) 117, 489
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 2 cut(s) 68, 74
TseI GCWGC 3 cut(s) 310, 313, 441
Tsp45I GTSAC 2 cut(s) 68, 74
TspDTI ATGAA 2 cut(s) 123, 135
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 1 cut(s) 85
VpaK11BI GGWCC 3 cut(s) 31, 214, 284
XmaJI CCTAGG 1 cut(s) 188
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.