Rh4BG308500

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000301
Physical Location & Seq
Reverse (-)
47766147 .. 47766581
435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG308500.1

Sequence Viewer

Length: 435 bp
ATGGCTTTAGCTGCCATTACTCTTTCCGCATCAACCCCGGAAGAAGATCAACAGGAATGGTTCTCCGATGAGGAAAATGATACTCCAAATGAACCATCCGATGATCTCCCTGCGACCTTAAGCCAACCAAAAACTTCCCTTAGGGGAGCAAGCCGCTTCCTTGCCACTCGGGCTGTTGCGACTACCTGTGACAAAAACCCTAAGGTTTGTAAGGCTGCAGGCAGTGCGGGGCGAGATTGCTGCAAGAAGAAGTGTGTGGATTTGAAGACGGACAGAGTCAATTGCGGCAAGTGTGGGAAGAAATGCAAGTACTCGGAGATATGCTGCAAAGGCAAGTGTCTGAATCCAATGGCTGACAAGAAAAACTGTGGGAGCTGCAACAACAAGTGCAAGAAAGGCAGTTCGTGTGTGTACGGAATGTGCAGCTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

144

Amino Acids

15.46

Weight (kDa)

8.66

Isoelectric Point (pI)

32.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 61 - 144 1.6e-29 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 27, 154, 227, 285
AfaI GTAC 2 cut(s) 311, 413
AflII CTTAAG 1 cut(s) 118
AgsI TTSAA 1 cut(s) 265
AluBI AGCT 3 cut(s) 11, 375, 426
AluI AGCT 3 cut(s) 11, 375, 426
Ama87I CYCGRG 1 cut(s) 168
ApeKI GCWGC 6 cut(s) 11, 215, 240, 324, 375, 423
AsuC2I CCSGG 1 cut(s) 38
AvaI CYCGRG 1 cut(s) 168
AxyI CCTNAGG 2 cut(s) 140, 201
BbsI GAAGAC 1 cut(s) 272
BbvI GCAGC 4 cut(s) 202, 227, 311, 362
BccI CCATC 1 cut(s) 103
BcgI CGANNNNNNTGC 2 cut(s) 222, 256
BcnI CCSGG 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 216
BfrI CTTAAG 1 cut(s) 118
BglI GCCNNNNNGGC 1 cut(s) 170
BisI GCNGC 8 cut(s) 12, 154, 216, 241, 286, 325, 376, 424
BlsI GCNGC 8 cut(s) 13, 155, 217, 242, 287, 326, 377, 425
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 1 cut(s) 38
BmeT110I CYCGRG 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 1 cut(s) 38
BpiI GAAGAC 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 36
Bse21I CCTNAGG 2 cut(s) 140, 201
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 1 cut(s) 95
BseXI GCAGC 4 cut(s) 202, 227, 311, 362
BsiHKCI CYCGRG 1 cut(s) 168
BsiSI CCGG 1 cut(s) 38
BsoBI CYCGRG 1 cut(s) 168
Bsp143I GATC 2 cut(s) 46, 103
BspACI CCGC 4 cut(s) 27, 154, 227, 285
BspMAI CTGCAG 1 cut(s) 220
BspTI CTTAAG 1 cut(s) 118
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 2 cut(s) 46, 103
Bst4CI ACNGT 1 cut(s) 368
BstAFI CTTAAG 1 cut(s) 118
BstAPI GCANNNNNTGC 1 cut(s) 224
BstC8I GCNNGC 2 cut(s) 151, 220
BstDEI CTNAG 2 cut(s) 140, 201
BstF5I GGATG 1 cut(s) 95
BstKTI GATC 2 cut(s) 49, 106
BstMBI GATC 2 cut(s) 46, 103
BstMWI GCNNNNNNNGC 5 cut(s) 11, 170, 224, 330, 396
BstSCI CCNGG 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 4 cut(s) 202, 227, 311, 362
BstV2I GAAGAC 1 cut(s) 272
Bsu36I CCTNAGG 2 cut(s) 140, 201
BtsCI GGATG 1 cut(s) 95
BtsI GCAGTG 1 cut(s) 229
BtsIMutI CAGTG 1 cut(s) 229
Cac8I GCNNGC 2 cut(s) 151, 220
Csp6I GTAC 2 cut(s) 310, 412
CviJI RGCY 9 cut(s) 5, 11, 123, 153, 173, 215, 353, 375, 426
CviKI_1 RGCY 9 cut(s) 5, 11, 123, 153, 173, 215, 353, 375, 426
CviQI GTAC 2 cut(s) 310, 412
DdeI CTNAG 2 cut(s) 140, 201
DpnI GATC 2 cut(s) 48, 105
DpnII GATC 2 cut(s) 46, 103
Eco81I CCTNAGG 2 cut(s) 140, 201
Eco88I CYCGRG 1 cut(s) 168
EcoT22I ATGCAT 1 cut(s) 433
FaiI YATR 3 cut(s) 322, 429, 433
FauI CCCGC 1 cut(s) 220
Fnu4HI GCNGC 8 cut(s) 12, 154, 216, 241, 286, 325, 376, 424
FokI GGATG 1 cut(s) 82
Fsp4HI GCNGC 8 cut(s) 12, 154, 216, 241, 286, 325, 376, 424
GluI GCNGC 8 cut(s) 12, 154, 216, 241, 286, 325, 376, 424
HapII CCGG 1 cut(s) 38
HinfI GANTC 2 cut(s) 276, 343
HpaII CCGG 1 cut(s) 38
Hpy166II GTNNAC 1 cut(s) 412
Hpy188I TCNGA 4 cut(s) 67, 100, 316, 342
Hpy8I GTNNAC 1 cut(s) 412
HpyCH4III ACNGT 1 cut(s) 368
HpyCH4V TGCA 8 cut(s) 218, 243, 306, 327, 378, 390, 423, 431
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 170, 224, 330, 396
HpyF3I CTNAG 2 cut(s) 140, 201
Kzo9I GATC 2 cut(s) 46, 103
LmnI GCTCC 2 cut(s) 146, 372
LpnPI CCDG 5 cut(s) 38, 51, 123, 199, 204
Lsp1109I GCAGC 4 cut(s) 202, 227, 311, 362
LweI GCATC 1 cut(s) 38
MaeIII GTNAC 1 cut(s) 188
MalI GATC 2 cut(s) 48, 105
MboI GATC 2 cut(s) 46, 103
MboII GAAGA 5 cut(s) 53, 56, 259, 277, 310
MfeI CAATTG 1 cut(s) 280
MluCI AATT 1 cut(s) 280
MlyI GAGTC 1 cut(s) 285
MnlI CCTC 1 cut(s) 64
Mph1103I ATGCAT 1 cut(s) 433
MseI TTAA 1 cut(s) 119
MspCI CTTAAG 1 cut(s) 118
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 38
MunI CAATTG 1 cut(s) 280
MwoI GCNNNNNNNGC 5 cut(s) 11, 170, 224, 330, 396
NciI CCSGG 1 cut(s) 38
NdeII GATC 2 cut(s) 46, 103
NmuCI GTSAC 1 cut(s) 188
NsiI ATGCAT 1 cut(s) 433
PfeI GAWTC 1 cut(s) 343
PflFI GACNNNGTC 1 cut(s) 275
PkrI GCNGC 8 cut(s) 13, 155, 217, 242, 287, 326, 377, 425
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
PstI CTGCAG 1 cut(s) 220
PsyI GACNNNGTC 1 cut(s) 275
RsaI GTAC 2 cut(s) 311, 413
RsaNI GTAC 2 cut(s) 310, 412
SaqAI TTAA 1 cut(s) 119
SatI GCNGC 8 cut(s) 12, 154, 216, 241, 286, 325, 376, 424
Sau3AI GATC 2 cut(s) 46, 103
ScaI AGTACT 1 cut(s) 311
SchI GAGTC 1 cut(s) 285
ScrFI CCNGG 1 cut(s) 38
SetI ASST 6 cut(s) 13, 119, 188, 207, 377, 428
SfaNI GCATC 1 cut(s) 38
SfcI CTRYAG 1 cut(s) 216
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
Sse9I AATT 1 cut(s) 280
SsiI CCGC 4 cut(s) 27, 154, 227, 285
StyD4I CCNGG 1 cut(s) 36
TaaI ACNGT 1 cut(s) 368
TasI AATT 1 cut(s) 280
TatI WGTACW 1 cut(s) 309
TauI GCSGC 2 cut(s) 156, 288
TfiI GAWTC 1 cut(s) 343
Tru1I TTAA 1 cut(s) 119
Tru9I TTAA 1 cut(s) 119
TscAI CASTG 1 cut(s) 229
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 6 cut(s) 11, 215, 240, 324, 375, 423
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 1 cut(s) 105
TspGWI ACGGA 2 cut(s) 284, 429
TspRI CASTG 1 cut(s) 229
Tth111I GACNNNGTC 1 cut(s) 275
Vha464I CTTAAG 1 cut(s) 118
ZrmI AGTACT 1 cut(s) 311
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.